smc1a cDNA ORF clone, Pygocentrus nattereri(red-bellied piranha)

Gene
Clones
gRNAs

Summary

Gene Symbol	smc1a
Entrez Gene ID	108433516
Full Name	structural maintenance of chromosomes 1A
Gene Type	protein-coding
Organism	Pygocentrus nattereri(red-bellied piranha)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017708140.1	XP_017563629.1	structural maintenance of chromosomes protein 1A isoform X1
XM_017708148.1	XP_017563637.1	structural maintenance of chromosomes protein 1A isoform X2

Pathways from BioSystems
Gene Ontology

The following smc1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smc1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPy22106	XM_017708140.1 Latest version!	Pygocentrus nattereri structural maintenance of chromosomes 1A (smc1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPy22107	XM_017708148.1 Latest version!	Pygocentrus nattereri structural maintenance of chromosomes 1A (smc1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smc1a ( XM_017708140.1 ) cDNA ORF clone, Pygocentrus nattereri(red-bellied piranha) -> XP_017563629.1 Pygocentrus nattereri structural maintenance of chromosomes 1A (smc1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPy22106
Clone ID Related Accession (Same CDS sequence)	XM_017708140.1
Accession Version	XM_017708140.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-01
Organism	Pygocentrus nattereri(red-bellied piranha)
Product	structural maintenance of chromosomes protein 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016243760.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pygocentrus nattereri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTACT TAAAACTAAT CGAGATCGAA AATTTCAAAT CGTACAAAGG TCGACAAATA 
ATAGGACCTT TCCACAAATT CACAGCGATA ATTGGACCGA ATGGATCAGG GAAGTCAAAC 
TTGATGGACG CCATCAGCTT TGTTTTAGCA GAGAAGACGA GTAATCTCCG TGTTAAAACC 
CTGAAGGACC TGATCCATGG CGCTCCAGTG GGGAAACCTG CAGCTAACCG TGCCTTTGTG 
AGCATGGTGT ACTGCGAGGA CAATGACAGT GAACGCACAT TCACACGGGT CATAATAGGT 
TCCTCTTCAG AATACAGGCT TGACAATAAG GTGGTTGGAC TCTCTGACTA CAGTGAGGAA 
CTGGAGAAAC TGGGCATCCT CATCAAGGCC AGGAACTTTC TGGTGTTTCA GGGTGCTGTT 
GAGTCCATAG CCATGAAGAA CCCAAAAGAG AGGACAGCGC TGTTTGAGGA GATTTCCCGC 
TCGGGAGAAC TAGCTCAGGA GTACGACCGC AGGAAGAAAG AGATGGTGAA GGCAGAGGAG 
GACACACAGT TCAACTATCA TCGGAAGAAG AACATCGCAG CTGAGCGCAA GGAGGCCAAG 
CAGGAGAAAG AGGAGGCTGA GCGGTACCAG CGGCTGAAGG ACGAGGTGAT TAGGGCTCAT 
GTACAGTTGC AGCTCTTCAA GCTCTACCAC AATGAAGCGG AGATTGAGAA GCTGAATCGA 
GAGCTGTCAC AGCGCAACCG TGAGATTGAG AAAGACAGAA AGAAAATGGA CCATATAGAA 
GACGAACTGA AGGAGAAGAA GAAAGAGCTG GGGAGGATGA TGAGGGACCA GCAGAACGTG 
GAGAAAGAGA TAAAAGAGAA AGATGCAGAG CTGAACCAGA AACGGCCGCT GTACATCAAA 
GCCAAGGAGA ACACCTCCCA CAAGATAAAG AAGCTGGAGG CAGCCCGCAA GTCTTTGCAG 
AATGCTCAGA AGATGTATAA GAAGCGTAAG GCAGATATGG AGGAGCTGGA CCAAGAGCAG 
GGGGCTGTGG AGATGTCCAG GCAGGAGTTT GAGGAGCGCA TGGAGGAGGA GGCTCAGAGT 
CAGGGCCAGG ACCTCACCCT GGAGGAGAAC CAGGTGAAGC AGTACCACAG GCTGAAAGAG 
GAGGCCAGTA AGCGGGCAGC CACGCTCGCA CAGGAGCTAG AGAAGTTCAA CCGTGATCAA 
AAGGCTGACC AGGACAGACT GGACCTCGAG GAGAGGAAGA AAGTGGAGAC AGAGGCTAAA 
ATAAAGCAAA AAATAAGAGA GATAGAGGAA AACCAGAAAC GCATTGAGAA GCTGGAAGAC 
TACATTGCGA CTAGCAGGCA GTCTCTGGAT GAACAGAGGA GAATGGAGGA GGAGTTGACT 
GAGGAAGTAG AGCTTGCCAA ACGGAGGATC GATGAGATAA ATATGGAATT AAACCAGGTG 
ATGGAGCAGC TTGGAGATGC CAGAATTGAC CGGCAGGAAA ACAGTCGCCA GCAGCGCAAA 
GCTGAGATCA TGGAGAGCAT TAAGAGACTC TACCCTGGTT CAGTGTATGG CAGACTGATT 
GACCTGTGCC AGCCCACACA GAAGAAGTAT CAGATTGCTG TGACAAAGGT GCTGGGAAAG 
AACATGGATG CCATCATTGT GGACTCAGAG AAGACGGGCA GAGACTGCAT CCAGTACATC 
AAAGAACAGA GAGGAGAACC AGAGACCTTC CTTCCCCTGG ACTACCTGGA AGTCAAGCCC 
ACAGATGAGA AGTTGAGGGA GCTGCGAGAA GCCAAGCTGG TGATTGACGT GATCCGCTAT 
GAGCCGCCCC ACATAAAGAA AGCACTACAG TATGCCTGTG GAAATGCTCT CGTGTGTGAG 
AATGTGGAGG ATGCTCGCAG GATTGCTTTT GGAGGACCTT ACCGACATAA GACTGTGGCC 
TTGGATGGCA CTCTTTTTCA AAAGTCTGGG GTGATCTCTG GAGGAGCGAG TGATCTGAAA 
GCGAAGGCCC GACGCTGGGA TGAGAAGGCT GTGGACAAAC TCAAAGATAA GAAAGAGAAA 
CTAACAGAGG AACTGAAGGA GCAGATGAAG GCGAAGAGGA AAGAGGCAGA GCTGAGGCAG 
GTCCAATCTC AGGCCCACGG GCTGCAGATG AGACTGAAGT ATTCTCAGAG TGACCTGGAA 
CAGACCAAGA CTCGCCACCT GTCCCTCAAC ATGCAGGAGA AATCAAAGCT GGAGAGTGAA 
TTGGCAAATT TTGGTCCGCG CATCAACGAC ATCAGGAGAA TCATCCAGTC ACGTGAGCGA 
GACATCACAG AGTTGAGAGA CCGCATGAAC CTGGTGGAGG ATGAAGTATT CCTTGAATTT 
TGTGAAGAGA TCGGGGTGAG AAACATTCGA GAGTTTGAAG AGGAGAAAGT CAAGAGACAG 
AATGAGATTG CCAAAAAACG ACTTGAATTT GAGACCCAGA AGACCCGTCT AGCCATCCAG 
CTAGACTATG AGCGGAACCA GCTAAAGGAG GACCAGGAGA AAGTGATGAT GTGGGAGCAG 
ACAGTGAAGA AAGATGAGAG TGAGATTGAA AGACTTAAGA AGGAGGAACA CAGACACATG 
AAAATCATTG ATGAGACCAT GGCCCAACTG CAGGACCTCA AAAATCAGCA CCTCACTAAA 
AAGGGAGAGG TCAATGACAA GAACCGAGAG ATGGAGGAGA TCCGCAAGAA GCTAGGAGCT 
GCTAACAAAG AGCTGACTCA GCTACAGAAA GAGGTGACAG CGATTGAGAC AAAGCTGGAG 
CAGAAACGCA GTGACAGACA CAACCTTCTA CAGGCCTGCA AGATGCAGGA CATCAAACTG 
CCCCTCAAAT CTGGAACCAT GGATGACATC AGCCAGGGAG AGGGAAGCTC CCAGGTTGAT 
GAGTCCAGCA ACCAGAAAAC ATCCAGCTCT GTGCATGCCA AGGAAGCTCT CATAGAAATA 
GATTACACCA ACCTAAATGA GGACCTTAAG GATGCTCTCT CTGAGGAGGA GATTAAGGCT 
GAGATGAACA CTTTGCAGCA GAGGCTGAAT GAGCAGCAGA GCATTCTCCA GCGCATCAGT 
GCTCCCAACA TGAAGGCCAT GGAGAAACTG GAGAGCGTCA GGGACAAGTT CCAGGAGACC 
AGTGACGAAT TTGAAGCAGC CCGGAAGAGA GCTAAGAAGG CTAAGCAGGC GTTTGAGCAG 
ATCAAGAAGG AGAGGTTTGA CCGTTTCAAC ACTTGCTTTG AGTCCGTTGC TACCAATATT 
GATGAGATCT ACAAAGCCCT CTCACGTAAC AGCAGCGCAC AGGCCTTTCT TGGCCCAGAG 
AACCCAGAGG AACCATACTT GGATGGAATC AATTACAATT GTGTGGCTCC AGGAAAGAGA 
TTTAGGCCAA TGGATAATCT GTCTGGAGGG GAGAAGACAG TAGCAGCTTT GGCTCTGCTT 
TTTGCCATTC ACAGCTACAA ACCGGCTCCG TTCTTTGTCC TTGATGAGAT TGATGCTGCG 
CTAGATAACA CAAACATTGG CAAGGTGGCC AATTACATAA AGGACCAGTC AGTCCAGAAC 
TTCCAGGCCA TTGTTATCTC CCTAAAAGAG GAGTTCTATA CAAAGGCTGA CTCACTCATA 
GGAGTTTACC CTGAGCAAGG AGACTGCGTC ATCAGCAAAG TGCTGACCTT TGACCTCTCC 
CAGTATCCTG ACGCCAATCC AAATCCCAAC GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017563629.1
CDS	167..3862
Translation	MGYLKLIEIENFKSYKGRQIIGPFHKFTAIIGPNGSGKSNLMDAISFVLAEKTSNLRVKTLKDLIHGAPVGKPAANRAFVSMVYCEDNDSERTFTRVIIGSSSEYRLDNKVVGLSDYSEELEKLGILIKARNFLVFQGAVESIAMKNPKERTALFEEISRSGELAQEYDRRKKEMVKAEEDTQFNYHRKKNIAAERKEAKQEKEEAERYQRLKDEVIRAHVQLQLFKLYHNEAEIEKLNRELSQRNREIEKDRKKMDHIEDELKEKKKELGRMMRDQQNVEKEIKEKDAELNQKRPLYIKAKENTSHKIKKLEAARKSLQNAQKMYKKRKADMEELDQEQGAVEMSRQEFEERMEEEAQSQGQDLTLEENQVKQYHRLKEEASKRAATLAQELEKFNRDQKADQDRLDLEERKKVETEAKIKQKIREIEENQKRIEKLEDYIATSRQSLDEQRRMEEELTEEVELAKRRIDEINMELNQVMEQLGDARIDRQENSRQQRKAEIMESIKRLYPGSVYGRLIDLCQPTQKKYQIAVTKVLGKNMDAIIVDSEKTGRDCIQYIKEQRGEPETFLPLDYLEVKPTDEKLRELREAKLVIDVIRYEPPHIKKALQYACGNALVCENVEDARRIAFGGPYRHKTVALDGTLFQKSGVISGGASDLKAKARRWDEKAVDKLKDKKEKLTEELKEQMKAKRKEAELRQVQSQAHGLQMRLKYSQSDLEQTKTRHLSLNMQEKSKLESELANFGPRINDIRRIIQSRERDITELRDRMNLVEDEVFLEFCEEIGVRNIREFEEEKVKRQNEIAKKRLEFETQKTRLAIQLDYERNQLKEDQEKVMMWEQTVKKDESEIERLKKEEHRHMKIIDETMAQLQDLKNQHLTKKGEVNDKNREMEEIRKKLGAANKELTQLQKEVTAIETKLEQKRSDRHNLLQACKMQDIKLPLKSGTMDDISQGEGSSQVDESSNQKTSSSVHAKEALIEIDYTNLNEDLKDALSEEEIKAEMNTLQQRLNEQQSILQRISAPNMKAMEKLESVRDKFQETSDEFEAARKRAKKAKQAFEQIKKERFDRFNTCFESVATNIDEIYKALSRNSSAQAFLGPENPEEPYLDGINYNCVAPGKRFRPMDNLSGGEKTVAALALLFAIHSYKPAPFFVLDEIDAALDNTNIGKVANYIKDQSVQNFQAIVISLKEEFYTKADSLIGVYPEQGDCVISKVLTFDLSQYPDANPNPND

Target ORF information:

RefSeq Version	XM_017708140.1
Organism	Pygocentrus nattereri(red-bellied piranha)
Definition	Pygocentrus nattereri structural maintenance of chromosomes 1A (smc1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017708140.1

ORF Insert Sequence:

ATGGGTTACT TAAAACTAAT CGAGATCGAA AATTTCAAAT CGTACAAAGG TCGACAAATA 
ATAGGACCTT TCCACAAATT CACAGCGATA ATTGGACCGA ATGGATCAGG GAAGTCAAAC 
TTGATGGACG CCATCAGCTT TGTTTTAGCA GAGAAGACGA GTAATCTCCG TGTTAAAACC 
CTGAAGGACC TGATCCATGG CGCTCCAGTG GGGAAACCTG CAGCTAACCG TGCCTTTGTG 
AGCATGGTGT ACTGCGAGGA CAATGACAGT GAACGCACAT TCACACGGGT CATAATAGGT 
TCCTCTTCAG AATACAGGCT TGACAATAAG GTGGTTGGAC TCTCTGACTA CAGTGAGGAA 
CTGGAGAAAC TGGGCATCCT CATCAAGGCC AGGAACTTTC TGGTGTTTCA GGGTGCTGTT 
GAGTCCATAG CCATGAAGAA CCCAAAAGAG AGGACAGCGC TGTTTGAGGA GATTTCCCGC 
TCGGGAGAAC TAGCTCAGGA GTACGACCGC AGGAAGAAAG AGATGGTGAA GGCAGAGGAG 
GACACACAGT TCAACTATCA TCGGAAGAAG AACATCGCAG CTGAGCGCAA GGAGGCCAAG 
CAGGAGAAAG AGGAGGCTGA GCGGTACCAG CGGCTGAAGG ACGAGGTGAT TAGGGCTCAT 
GTACAGTTGC AGCTCTTCAA GCTCTACCAC AATGAAGCGG AGATTGAGAA GCTGAATCGA 
GAGCTGTCAC AGCGCAACCG TGAGATTGAG AAAGACAGAA AGAAAATGGA CCATATAGAA 
GACGAACTGA AGGAGAAGAA GAAAGAGCTG GGGAGGATGA TGAGGGACCA GCAGAACGTG 
GAGAAAGAGA TAAAAGAGAA AGATGCAGAG CTGAACCAGA AACGGCCGCT GTACATCAAA 
GCCAAGGAGA ACACCTCCCA CAAGATAAAG AAGCTGGAGG CAGCCCGCAA GTCTTTGCAG 
AATGCTCAGA AGATGTATAA GAAGCGTAAG GCAGATATGG AGGAGCTGGA CCAAGAGCAG 
GGGGCTGTGG AGATGTCCAG GCAGGAGTTT GAGGAGCGCA TGGAGGAGGA GGCTCAGAGT 
CAGGGCCAGG ACCTCACCCT GGAGGAGAAC CAGGTGAAGC AGTACCACAG GCTGAAAGAG 
GAGGCCAGTA AGCGGGCAGC CACGCTCGCA CAGGAGCTAG AGAAGTTCAA CCGTGATCAA 
AAGGCTGACC AGGACAGACT GGACCTCGAG GAGAGGAAGA AAGTGGAGAC AGAGGCTAAA 
ATAAAGCAAA AAATAAGAGA GATAGAGGAA AACCAGAAAC GCATTGAGAA GCTGGAAGAC 
TACATTGCGA CTAGCAGGCA GTCTCTGGAT GAACAGAGGA GAATGGAGGA GGAGTTGACT 
GAGGAAGTAG AGCTTGCCAA ACGGAGGATC GATGAGATAA ATATGGAATT AAACCAGGTG 
ATGGAGCAGC TTGGAGATGC CAGAATTGAC CGGCAGGAAA ACAGTCGCCA GCAGCGCAAA 
GCTGAGATCA TGGAGAGCAT TAAGAGACTC TACCCTGGTT CAGTGTATGG CAGACTGATT 
GACCTGTGCC AGCCCACACA GAAGAAGTAT CAGATTGCTG TGACAAAGGT GCTGGGAAAG 
AACATGGATG CCATCATTGT GGACTCAGAG AAGACGGGCA GAGACTGCAT CCAGTACATC 
AAAGAACAGA GAGGAGAACC AGAGACCTTC CTTCCCCTGG ACTACCTGGA AGTCAAGCCC 
ACAGATGAGA AGTTGAGGGA GCTGCGAGAA GCCAAGCTGG TGATTGACGT GATCCGCTAT 
GAGCCGCCCC ACATAAAGAA AGCACTACAG TATGCCTGTG GAAATGCTCT CGTGTGTGAG 
AATGTGGAGG ATGCTCGCAG GATTGCTTTT GGAGGACCTT ACCGACATAA GACTGTGGCC 
TTGGATGGCA CTCTTTTTCA AAAGTCTGGG GTGATCTCTG GAGGAGCGAG TGATCTGAAA 
GCGAAGGCCC GACGCTGGGA TGAGAAGGCT GTGGACAAAC TCAAAGATAA GAAAGAGAAA 
CTAACAGAGG AACTGAAGGA GCAGATGAAG GCGAAGAGGA AAGAGGCAGA GCTGAGGCAG 
GTCCAATCTC AGGCCCACGG GCTGCAGATG AGACTGAAGT ATTCTCAGAG TGACCTGGAA 
CAGACCAAGA CTCGCCACCT GTCCCTCAAC ATGCAGGAGA AATCAAAGCT GGAGAGTGAA 
TTGGCAAATT TTGGTCCGCG CATCAACGAC ATCAGGAGAA TCATCCAGTC ACGTGAGCGA 
GACATCACAG AGTTGAGAGA CCGCATGAAC CTGGTGGAGG ATGAAGTATT CCTTGAATTT 
TGTGAAGAGA TCGGGGTGAG AAACATTCGA GAGTTTGAAG AGGAGAAAGT CAAGAGACAG 
AATGAGATTG CCAAAAAACG ACTTGAATTT GAGACCCAGA AGACCCGTCT AGCCATCCAG 
CTAGACTATG AGCGGAACCA GCTAAAGGAG GACCAGGAGA AAGTGATGAT GTGGGAGCAG 
ACAGTGAAGA AAGATGAGAG TGAGATTGAA AGACTTAAGA AGGAGGAACA CAGACACATG 
AAAATCATTG ATGAGACCAT GGCCCAACTG CAGGACCTCA AAAATCAGCA CCTCACTAAA 
AAGGGAGAGG TCAATGACAA GAACCGAGAG ATGGAGGAGA TCCGCAAGAA GCTAGGAGCT 
GCTAACAAAG AGCTGACTCA GCTACAGAAA GAGGTGACAG CGATTGAGAC AAAGCTGGAG 
CAGAAACGCA GTGACAGACA CAACCTTCTA CAGGCCTGCA AGATGCAGGA CATCAAACTG 
CCCCTCAAAT CTGGAACCAT GGATGACATC AGCCAGGGAG AGGGAAGCTC CCAGGTTGAT 
GAGTCCAGCA ACCAGAAAAC ATCCAGCTCT GTGCATGCCA AGGAAGCTCT CATAGAAATA 
GATTACACCA ACCTAAATGA GGACCTTAAG GATGCTCTCT CTGAGGAGGA GATTAAGGCT 
GAGATGAACA CTTTGCAGCA GAGGCTGAAT GAGCAGCAGA GCATTCTCCA GCGCATCAGT 
GCTCCCAACA TGAAGGCCAT GGAGAAACTG GAGAGCGTCA GGGACAAGTT CCAGGAGACC 
AGTGACGAAT TTGAAGCAGC CCGGAAGAGA GCTAAGAAGG CTAAGCAGGC GTTTGAGCAG 
ATCAAGAAGG AGAGGTTTGA CCGTTTCAAC ACTTGCTTTG AGTCCGTTGC TACCAATATT 
GATGAGATCT ACAAAGCCCT CTCACGTAAC AGCAGCGCAC AGGCCTTTCT TGGCCCAGAG 
AACCCAGAGG AACCATACTT GGATGGAATC AATTACAATT GTGTGGCTCC AGGAAAGAGA 
TTTAGGCCAA TGGATAATCT GTCTGGAGGG GAGAAGACAG TAGCAGCTTT GGCTCTGCTT 
TTTGCCATTC ACAGCTACAA ACCGGCTCCG TTCTTTGTCC TTGATGAGAT TGATGCTGCG 
CTAGATAACA CAAACATTGG CAAGGTGGCC AATTACATAA AGGACCAGTC AGTCCAGAAC 
TTCCAGGCCA TTGTTATCTC CCTAAAAGAG GAGTTCTATA CAAAGGCTGA CTCACTCATA 
GGAGTTTACC CTGAGCAAGG AGACTGCGTC ATCAGCAAAG TGCTGACCTT TGACCTCTCC 
CAGTATCCTG ACGCCAATCC AAATCCCAAC GATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smc1a ( XM_017708148.1 ) cDNA ORF clone, Pygocentrus nattereri(red-bellied piranha) -> XP_017563637.1 Pygocentrus nattereri structural maintenance of chromosomes 1A (smc1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPy22107
Clone ID Related Accession (Same CDS sequence)	XM_017708148.1
Accession Version	XM_017708148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-01
Organism	Pygocentrus nattereri(red-bellied piranha)
Product	structural maintenance of chromosomes protein 1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016243760.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pygocentrus nattereri Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTACT TAAAACTAAT CGAGATCGAA AATTTCAAAT CGTACAAAGG TCGACAAATA 
ATAGGACCTT TCCACAAATT CACAGCGATA ATTGGACCGA ATGGATCAGG GAAGTCAAAC 
TTGATGGACG CCATCAGCTT TGTTTTAGCA GAGAAGACGA GTAATCTCCG TGTTAAAACC 
CTGAAGGACC TGATCCATGG CGCTCCAGTG GGGAAACCTG CAGCTAACCG TGCCTTTGTG 
AGCATGGTGT ACTGCGAGGA CAATGACAGT GAACGCACAT TCACACGGGT CATAATAGGT 
TCCTCTTCAG AATACAGGCT TGACAATAAG GTGGTTGGAC TCTCTGACTA CAGTGAGGAA 
CTGGAGAAAC TGGGCATCCT CATCAAGGCC AGGAACTTTC TGGTGTTTCA GGGTGCTGTT 
GAGTCCATAG CCATGAAGAA CCCAAAAGAG AGGACAGCGC TGTTTGAGGA GATTTCCCGC 
TCGGGAGAAC TAGCTCAGGA GTACGACCGC AGGAAGAAAG AGATGGTGAA GGCAGAGGAG 
GACACACAGT TCAACTATCA TCGGAAGAAG AACATCGCAG CTGAGCGCAA GGAGGCCAAG 
CAGGAGAAAG AGGAGGCTGA GCGGTACCAG CGGCTGAAGG ACGAGGTGAT TAGGGCTCAT 
GTACAGTTGC AGCTCTTCAA GCTCTACCAC AATGAAGCGG AGATTGAGAA GCTGAATCGA 
GAGCTGTCAC AGCGCAACCG TGAGATTGAG AAAGACAGAA AGAAAATGGA CCATATAGAA 
GACGAACTGA AGGAGAAGAA GAAAGAGCTG GGGAGGATGA TGAGGGACCA GCAGAACGTG 
GAGAAAGAGA TAAAAGAGAA AGATGCAGAG CTGAACCAGA AACGGCCGCT GTACATCAAA 
GCCAAGGAGA ACACCTCCCA CAAGATAAAG AAGCTGGAGG CAGCCCGCAA GTCTTTGCAG 
AATGCTCAGA AGATGTATAA GAAGCGTAAG GCAGATATGG AGGAGCTGGA CCAAGAGCAG 
GGGGCTGTGG AGATGTCCAG GCAGGAGTTT GAGGAGCGCA TGGAGGAGGA GGCTCAGAGT 
CAGGGCCAGG ACCTCACCCT GGAGGAGAAC CAGGTGAAGC AGTACCACAG GCTGAAAGAG 
GAGGCCAGTA AGCGGGCAGC CACGCTCGCA CAGGAGCTAG AGAAGTTCAA CCGTGATCAA 
AAGGCTGACC AGGACAGACT GGACCTCGAG GAGAGGAAGA AAGTGGAGAC AGAGGCTAAA 
ATAAAGCAAA AAATAAGAGA GATAGAGGAA AACCAGAAAC GCATTGAGAA GCTGGAAGAC 
TACATTGCGA CTAGCAGGCA GTCTCTGGAT GAACAGAGGA GAATGGAGGA GGAGTTGACT 
GAGGAAGTAG AGCTTGCCAA ACGGAGGATC GATGAGATAA ATATGGAATT AAACCAGGTG 
ATGGAGCAGC TTGGAGATGC CAGAATTGAC CGGCAGGAAA ACAGTCGCCA GCAGCGCAAA 
GCTGAGATCA TGGAGAGCAT TAAGAGACTC TACCCTGGTT CAGTGTATGG CAGACTGATT 
GACCTGTGCC AGCCCACACA GAAGAAGTAT CAGATTGCTG TGACAAAGGT GCTGGGAAAG 
AACATGGATG CCATCATTGT GGACTCAGAG AAGACGGGCA GAGACTGCAT CCAGTACATC 
AAAGAACAGA GAGGAGAACC AGAGACCTTC CTTCCCCTGG ACTACCTGGA AGTCAAGCCC 
ACAGATGAGA AGTTGAGGGA GCTGCGAGAA GCCAAGCTGG TGATTGACGT GATCCGCTAT 
GAGCCGCCCC ACATAAAGAA AGCACTACAG TATGCCTGTG GAAATGCTCT CGTGTGTGAG 
AATGTGGAGG ATGCTCGCAG GATTGCTTTT GGAGGACCTT ACCGACATAA GACTGTGGCC 
TTGGATGGCA CTCTTTTTCA AAAGTCTGGG GTGATCTCTG GAGGAGCGAG TGATCTGAAA 
GCGAAGGCCC GACGCTGGGA TGAGAAGGCT GTGGACAAAC TCAAAGATAA GAAAGAGAAA 
CTAACAGAGG AACTGAAGGA GCAGATGAAG GCGAAGAGGA AAGAGGCAGA GCTGAGGCAG 
GTCCAATCTC AGGCCCACGG GCTGCAGATG AGACTGAAGT ATTCTCAGAG TGACCTGGAA 
CAGACCAAGA CTCGCCACCT GTCCCTCAAC ATGCAGGAGA AATCAAAGCT GGAGAGTGAA 
TTGGCAAATT TTGGTCCGCG CATCAACGAC ATCAGGAGAA TCATCCAGTC ACGTGAGCGA 
GACATCACAG AGTTGAGAGA CCGCATGAAC CTGGTGGAGG ATGAAGTATT CCTTGAATTT 
TGTGAAGAGA TCGGGGTGAG AAACATTCGA GAGTTTGAAG AGGAGAAAGT CAAGAGACAG 
AATGAGATTG CCAAAAAACG ACTTGAATTT GAGACCCAGA AGACCCGTCT AGCCATCCAG 
CTAGACTATG AGCGGAACCA GCTAAAGGAG GACCAGGAGA AAGTGATGAT GTGGGAGCAG 
ACAGTGAAGA AAGATGAGAG TGAGATTGAA AGACTTAAGA AGGAGGAACA CAGACACATG 
AAAATCATTG ATGAGACCAT GGCCCAACTG CAGGACCTCA AAAATCAGCA CCTCACTAAA 
AAGGGAGAGG TCAATGACAA GAACCGAGAG ATGGAGGAGA TCCGCAAGAA GCTAGGAGCT 
GCTAACAAAG AGCTGACTCA GCTACAGAAA GAGGTGACAG CGATTGAGAC AAAGCTGGAG 
CAGAAACGCA GTGACAGACA CAACCTTCTA CAGGCCTGCA AGATGCAGGA CATCAAACTG 
CCCCTCAAAT CTGGAACCAT GGATGACATC AGCCAGGGAG AGGGAAGCTC CCAGGTTGAT 
GAGTCCAGCA ACCAGAAAAC ATCCAGCTCT GTGCATGCCA AGGAAGCTCT CATAGAAATA 
GATTACACCA ACCTAAATGA GGACCTTAAG GATGCTCTCT CTGAGGAGGA GATTAAGGCT 
GAGATGAACA CTTTGCAGCA GAGGCTGAAT GAGCAGCAGA GCATTCTCCA GCGCATCAGT 
GCTCCCAACA TGAAGGCCAT GGAGAAACTG GAGAGCGTCA GGGACAAGTT CCAGGAGACC 
AGTGACGAAT TTGAAGCAGC CCGGAAGAGA GCTAAGAAGG CTAAGCAGGC GTTTGAGCAG 
ATCAAGAAGG AGAGGTTTGA CCGTTTCAAC ACTTGCTTTG AGTCCGTTGC TACCAATATT 
GATGAGATCT ACAAAGCCCT CTCACGTAAC AGCAGCGCAC AGGCCTTTCT TGGCCCAGAG 
AACCCAGAGG AACCATACTT GGATGGAATC AATTACAATT GTGTGGCTCC AGGAAAGAGA 
TTTAGGCCAA TGGATAATCT GTCTGGAGGG GAGAAGACAG TAGCAGCTTT GGCTCTGCTT 
TTTGCCATTC ACAGGTGGCC AATTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017563637.1
CDS	167..3616
Translation	MGYLKLIEIENFKSYKGRQIIGPFHKFTAIIGPNGSGKSNLMDAISFVLAEKTSNLRVKTLKDLIHGAPVGKPAANRAFVSMVYCEDNDSERTFTRVIIGSSSEYRLDNKVVGLSDYSEELEKLGILIKARNFLVFQGAVESIAMKNPKERTALFEEISRSGELAQEYDRRKKEMVKAEEDTQFNYHRKKNIAAERKEAKQEKEEAERYQRLKDEVIRAHVQLQLFKLYHNEAEIEKLNRELSQRNREIEKDRKKMDHIEDELKEKKKELGRMMRDQQNVEKEIKEKDAELNQKRPLYIKAKENTSHKIKKLEAARKSLQNAQKMYKKRKADMEELDQEQGAVEMSRQEFEERMEEEAQSQGQDLTLEENQVKQYHRLKEEASKRAATLAQELEKFNRDQKADQDRLDLEERKKVETEAKIKQKIREIEENQKRIEKLEDYIATSRQSLDEQRRMEEELTEEVELAKRRIDEINMELNQVMEQLGDARIDRQENSRQQRKAEIMESIKRLYPGSVYGRLIDLCQPTQKKYQIAVTKVLGKNMDAIIVDSEKTGRDCIQYIKEQRGEPETFLPLDYLEVKPTDEKLRELREAKLVIDVIRYEPPHIKKALQYACGNALVCENVEDARRIAFGGPYRHKTVALDGTLFQKSGVISGGASDLKAKARRWDEKAVDKLKDKKEKLTEELKEQMKAKRKEAELRQVQSQAHGLQMRLKYSQSDLEQTKTRHLSLNMQEKSKLESELANFGPRINDIRRIIQSRERDITELRDRMNLVEDEVFLEFCEEIGVRNIREFEEEKVKRQNEIAKKRLEFETQKTRLAIQLDYERNQLKEDQEKVMMWEQTVKKDESEIERLKKEEHRHMKIIDETMAQLQDLKNQHLTKKGEVNDKNREMEEIRKKLGAANKELTQLQKEVTAIETKLEQKRSDRHNLLQACKMQDIKLPLKSGTMDDISQGEGSSQVDESSNQKTSSSVHAKEALIEIDYTNLNEDLKDALSEEEIKAEMNTLQQRLNEQQSILQRISAPNMKAMEKLESVRDKFQETSDEFEAARKRAKKAKQAFEQIKKERFDRFNTCFESVATNIDEIYKALSRNSSAQAFLGPENPEEPYLDGINYNCVAPGKRFRPMDNLSGGEKTVAALALLFAIHRWPIT

Target ORF information:

RefSeq Version	XM_017708148.1
Organism	Pygocentrus nattereri(red-bellied piranha)
Definition	Pygocentrus nattereri structural maintenance of chromosomes 1A (smc1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017708148.1

ORF Insert Sequence:

ATGGGTTACT TAAAACTAAT CGAGATCGAA AATTTCAAAT CGTACAAAGG TCGACAAATA 
ATAGGACCTT TCCACAAATT CACAGCGATA ATTGGACCGA ATGGATCAGG GAAGTCAAAC 
TTGATGGACG CCATCAGCTT TGTTTTAGCA GAGAAGACGA GTAATCTCCG TGTTAAAACC 
CTGAAGGACC TGATCCATGG CGCTCCAGTG GGGAAACCTG CAGCTAACCG TGCCTTTGTG 
AGCATGGTGT ACTGCGAGGA CAATGACAGT GAACGCACAT TCACACGGGT CATAATAGGT 
TCCTCTTCAG AATACAGGCT TGACAATAAG GTGGTTGGAC TCTCTGACTA CAGTGAGGAA 
CTGGAGAAAC TGGGCATCCT CATCAAGGCC AGGAACTTTC TGGTGTTTCA GGGTGCTGTT 
GAGTCCATAG CCATGAAGAA CCCAAAAGAG AGGACAGCGC TGTTTGAGGA GATTTCCCGC 
TCGGGAGAAC TAGCTCAGGA GTACGACCGC AGGAAGAAAG AGATGGTGAA GGCAGAGGAG 
GACACACAGT TCAACTATCA TCGGAAGAAG AACATCGCAG CTGAGCGCAA GGAGGCCAAG 
CAGGAGAAAG AGGAGGCTGA GCGGTACCAG CGGCTGAAGG ACGAGGTGAT TAGGGCTCAT 
GTACAGTTGC AGCTCTTCAA GCTCTACCAC AATGAAGCGG AGATTGAGAA GCTGAATCGA 
GAGCTGTCAC AGCGCAACCG TGAGATTGAG AAAGACAGAA AGAAAATGGA CCATATAGAA 
GACGAACTGA AGGAGAAGAA GAAAGAGCTG GGGAGGATGA TGAGGGACCA GCAGAACGTG 
GAGAAAGAGA TAAAAGAGAA AGATGCAGAG CTGAACCAGA AACGGCCGCT GTACATCAAA 
GCCAAGGAGA ACACCTCCCA CAAGATAAAG AAGCTGGAGG CAGCCCGCAA GTCTTTGCAG 
AATGCTCAGA AGATGTATAA GAAGCGTAAG GCAGATATGG AGGAGCTGGA CCAAGAGCAG 
GGGGCTGTGG AGATGTCCAG GCAGGAGTTT GAGGAGCGCA TGGAGGAGGA GGCTCAGAGT 
CAGGGCCAGG ACCTCACCCT GGAGGAGAAC CAGGTGAAGC AGTACCACAG GCTGAAAGAG 
GAGGCCAGTA AGCGGGCAGC CACGCTCGCA CAGGAGCTAG AGAAGTTCAA CCGTGATCAA 
AAGGCTGACC AGGACAGACT GGACCTCGAG GAGAGGAAGA AAGTGGAGAC AGAGGCTAAA 
ATAAAGCAAA AAATAAGAGA GATAGAGGAA AACCAGAAAC GCATTGAGAA GCTGGAAGAC 
TACATTGCGA CTAGCAGGCA GTCTCTGGAT GAACAGAGGA GAATGGAGGA GGAGTTGACT 
GAGGAAGTAG AGCTTGCCAA ACGGAGGATC GATGAGATAA ATATGGAATT AAACCAGGTG 
ATGGAGCAGC TTGGAGATGC CAGAATTGAC CGGCAGGAAA ACAGTCGCCA GCAGCGCAAA 
GCTGAGATCA TGGAGAGCAT TAAGAGACTC TACCCTGGTT CAGTGTATGG CAGACTGATT 
GACCTGTGCC AGCCCACACA GAAGAAGTAT CAGATTGCTG TGACAAAGGT GCTGGGAAAG 
AACATGGATG CCATCATTGT GGACTCAGAG AAGACGGGCA GAGACTGCAT CCAGTACATC 
AAAGAACAGA GAGGAGAACC AGAGACCTTC CTTCCCCTGG ACTACCTGGA AGTCAAGCCC 
ACAGATGAGA AGTTGAGGGA GCTGCGAGAA GCCAAGCTGG TGATTGACGT GATCCGCTAT 
GAGCCGCCCC ACATAAAGAA AGCACTACAG TATGCCTGTG GAAATGCTCT CGTGTGTGAG 
AATGTGGAGG ATGCTCGCAG GATTGCTTTT GGAGGACCTT ACCGACATAA GACTGTGGCC 
TTGGATGGCA CTCTTTTTCA AAAGTCTGGG GTGATCTCTG GAGGAGCGAG TGATCTGAAA 
GCGAAGGCCC GACGCTGGGA TGAGAAGGCT GTGGACAAAC TCAAAGATAA GAAAGAGAAA 
CTAACAGAGG AACTGAAGGA GCAGATGAAG GCGAAGAGGA AAGAGGCAGA GCTGAGGCAG 
GTCCAATCTC AGGCCCACGG GCTGCAGATG AGACTGAAGT ATTCTCAGAG TGACCTGGAA 
CAGACCAAGA CTCGCCACCT GTCCCTCAAC ATGCAGGAGA AATCAAAGCT GGAGAGTGAA 
TTGGCAAATT TTGGTCCGCG CATCAACGAC ATCAGGAGAA TCATCCAGTC ACGTGAGCGA 
GACATCACAG AGTTGAGAGA CCGCATGAAC CTGGTGGAGG ATGAAGTATT CCTTGAATTT 
TGTGAAGAGA TCGGGGTGAG AAACATTCGA GAGTTTGAAG AGGAGAAAGT CAAGAGACAG 
AATGAGATTG CCAAAAAACG ACTTGAATTT GAGACCCAGA AGACCCGTCT AGCCATCCAG 
CTAGACTATG AGCGGAACCA GCTAAAGGAG GACCAGGAGA AAGTGATGAT GTGGGAGCAG 
ACAGTGAAGA AAGATGAGAG TGAGATTGAA AGACTTAAGA AGGAGGAACA CAGACACATG 
AAAATCATTG ATGAGACCAT GGCCCAACTG CAGGACCTCA AAAATCAGCA CCTCACTAAA 
AAGGGAGAGG TCAATGACAA GAACCGAGAG ATGGAGGAGA TCCGCAAGAA GCTAGGAGCT 
GCTAACAAAG AGCTGACTCA GCTACAGAAA GAGGTGACAG CGATTGAGAC AAAGCTGGAG 
CAGAAACGCA GTGACAGACA CAACCTTCTA CAGGCCTGCA AGATGCAGGA CATCAAACTG 
CCCCTCAAAT CTGGAACCAT GGATGACATC AGCCAGGGAG AGGGAAGCTC CCAGGTTGAT 
GAGTCCAGCA ACCAGAAAAC ATCCAGCTCT GTGCATGCCA AGGAAGCTCT CATAGAAATA 
GATTACACCA ACCTAAATGA GGACCTTAAG GATGCTCTCT CTGAGGAGGA GATTAAGGCT 
GAGATGAACA CTTTGCAGCA GAGGCTGAAT GAGCAGCAGA GCATTCTCCA GCGCATCAGT 
GCTCCCAACA TGAAGGCCAT GGAGAAACTG GAGAGCGTCA GGGACAAGTT CCAGGAGACC 
AGTGACGAAT TTGAAGCAGC CCGGAAGAGA GCTAAGAAGG CTAAGCAGGC GTTTGAGCAG 
ATCAAGAAGG AGAGGTTTGA CCGTTTCAAC ACTTGCTTTG AGTCCGTTGC TACCAATATT 
GATGAGATCT ACAAAGCCCT CTCACGTAAC AGCAGCGCAC AGGCCTTTCT TGGCCCAGAG 
AACCCAGAGG AACCATACTT GGATGGAATC AATTACAATT GTGTGGCTCC AGGAAAGAGA 
TTTAGGCCAA TGGATAATCT GTCTGGAGGG GAGAAGACAG TAGCAGCTTT GGCTCTGCTT 
TTTGCCATTC ACAGGTGGCC AATTACATAA