DGKD cDNA ORF clone, Pteropus vampyrus(large flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKD
Entrez Gene ID	105306002
Full Name	diacylglycerol kinase delta
Gene Type	protein-coding
Organism	Pteropus vampyrus(large flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011380876.1	XP_011379178.1	diacylglycerol kinase delta
XM_011380876.2	XP_011379178.1	diacylglycerol kinase delta

Pathways from BioSystems
Gene Ontology

The following DGKD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt61710	XM_011380876.2 Latest version!	Pteropus vampyrus diacylglycerol kinase delta (DGKD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OPt61710	XM_011380876.1	Pteropus vampyrus diacylglycerol kinase, delta 130kDa (DGKD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKD ( XM_011380876.1 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011379178.1 Pteropus vampyrus diacylglycerol kinase, delta 130kDa (DGKD), mRNA.

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CloneID	OPt61710
Clone ID Related Accession (Same CDS sequence)	XM_011380876.1 , XM_011380876.2
Accession Version	XM_011380876.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-22
Organism	Pteropus vampyrus(large flying fox)
Product	diacylglycerol kinase delta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889192.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus vampyrus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCCGT TCCTGTGGTT TCAGACCGTC ATCAAAGAGG GGGTGCTGAG CAAACAGAAC 
AGCTCATTCC AGCGATCCAG AAGGAGGTAC TTCAAGCTTC GCGGGCGAAC GCTGTACTAT 
GCGAAGACGG CAAAGTCGAT CATATTTGAC GAGGTGGACC TGACAGATGC CAGCGTGGCT 
GAATCCAGCA CGAAAAACGT CAACAACAGT TTTACGGTCA TCACTCCATG CAGGAAGCTC 
ATCTTATGCG CCGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCTCT GAAGACTGTC 
CAGAACAGGG AGCATTTTGA GCCGACGCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AACTGGTACG CCTGCTCGCA CGCGAGGCCC ACCTACTGCA ACGTGTGCCG CGAGGCGCTG 
TCTGGGGTCA CGTCGCACGG CCTGTCCTGT GAAGTGTGCA AGTTTAAGGC CCACAAGCGG 
TGTGCTGTGC GTGCAACCAG TAACTGCAAG TGGACCACAC TGGCCTCCAT TGGGAAGGAC 
ATCATCGAGG ACGAGGATGG GGTCGCCATG CCTCACCAGT GGCTGGAAGG AAACCTGCCT 
GTGAGCGCCA AGTGCACAGT GTGCGACAAG ACCTGCGGCA GCGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTG CACACGGCGT GTAAGGAATC CTTGCTGACC 
AGGTGCCCGC TGGGCCTGTG TAAAGTGTCA GTCATCCCTC CCACGGCTCT CAACAGCATC 
GACTCCGATG GCTTCTGGAA GGCCACATGT CCTCCATCCT GCACAAGCCC CTTATTGGTC 
TTTGTCAATT CAAAAAGTGG GGACAACCAG GGCGTGAAGT TCCTCAGAAG GTTCAAGCAG 
CTGCTGAATC CTGCCCAGGT CTTCGACCTC ATGAATGGAG GCCCACACCT CGGCTTACGC 
TTATTCCAGA AGTTTGATAC CTTTCGGATT CTGGTGTGCG GTGGGGACGG CAGTGTCGGC 
TGGGTCCTCT CTGAAATTGA CAGCCTCAAT CTGCACAAAC AGTGTCAGCT GGGAGTGTTG 
CCGCTCGGCA CAGGGAACGA CCTGGCTCGT GTGTTAGGCT GGGGCTCGGC CTGTGACGAT 
GACACCCAGC TCCCGCAGAT CCTGGAGAAG CTGGAAAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCCTA TGAGACCAAA CCCCCCCGGC CGGCCTCCTC TTCCACCGTC 
GTCACCGAGG ACTTCAGCGA GGGTTCCGAG GTACAGCAGA TTCTCTTCTA TGAAGACTCT 
GTCGCGGCCC ATCTTTCTAA AATCCTGACC TCTGACCAGC ACTCGGTGGT GATATCATCG 
GCGAAGGTGC TCTGCGAGAC GGTGAAGGAC TTCGTGGCGC GAGTGGGCAA GGCCTATGAG 
AAGACAGCTG AGGGCTCGGA GGAGTCAGAG GCCGTGGCCA AGAAGTGCTC TGTCCTGAAA 
GAGAAGCTGG ATTCCCTCCT CAAGACTTTG GACGACGAGT CGCAGGCCTT GTCCTCTCTG 
CCCACCCCAC CGCCCACCAT TGCTGAGGAG GCGGAAGACG GAGACGGGCT GGGCAGCGGC 
TGTGGCTCCG CAGTGGACCG CTCGGTGGGG TCGGCGTGCC CAGCCCGGCC ACAGATATTC 
CGGCCTCGGG AACAACTCAT GCTAAGAGCC AACAGCCTGA AGAAGGCGAT CCGTCAGATC 
ATAGAACACA CAGAAAAAGC TGTCGACGAG CAGAACGCCC AGACCCAAGA ACAGGAGAGG 
CTTGTCTTGG GGCTCTCCGA GTCGGAGGGG AAGAAAGACC TGAAATCGGA TGACAGACTG 
TGCCACAGCG GGAGCTGCGG GGCGGCCAAG TGCAGGAGCC TCCGCAAAGT GTCCAGATCA 
CCATGTGAGA AGCTGATAAG CAAAGGAAGT TTGTCACTGG GCAGTTCTGC GTCTCTTCCT 
CCCCAGTCAG GAAATCGGGA CGGCCTGCCA GCACTGAACA CAAAGATCCT GTTTCCAAAC 
GTTCGGGCTG GGATGTCCGG TTCCTTGCCT GGAGGATCCG TCATCAGTCG CTTATTGATT 
AATGCTGATC CCTTCAACTC CGAACCAGAA AACCTAGAGT ACTACACAGA GAAGTGTGTC 
ATGAACAATT ACTTTGGCAT CGGCCTGGAT GCGAAGATAT CCCTGGACTT CAACAACAAG 
CGTGACGAAC ACCCGGAGAA GTGCAGGAGT CGAACCAAGA ACATGATGTG GTACGGGGTT 
CTCGGAACCA AGGAGCTGCT GCACAGAACC TACAAGAACC TGGAGCAGAA GGTCCTGCTG 
GAGTGTGATG GGCGGCCCAT CCCACTCCCC AGTCTCCAGG GAATTGCTGT CCTCAACATT 
CCCAGCTATG CCGGAGGGAC CAACTTCTGG GGGGGCACCA AAGAAGACGA TACTTTTGCA 
GCTCCCTCGT TCGATGATAA GATCTTGGAG GTGGTGGCCG TGTTTGGCAG TATGCAGATG 
GCTGTCTCTC GGGTCATTAA GCTGCAGCAT CACCGGATTG CCCAGTGTCG CACAGTGAAG 
ATTTCCATCC TGGGGGACGA GGGCGTGCCT GTGCAGGTGG ACGGAGAGGC CTGGATCCAG 
CCCCCAGGAT ACATTCGGAT CATTCACAAG AACCGGGCGC AGATGCTCAC CAGAGACAGG 
GCATTCGAGA GCACCCTGAA GTCCTGGGAG GACAAGCAGA AGTGCGAGCT GCCCCGCCCG 
CCGTCCTTCT CGCTGCACGC GGAGACCCTG TCCGAGGAGG AGGCCACACA GATGGAGCAG 
TTCGGGCAGG CGGCGGGAGC CCTCATCCAC AGCATCCGGG AGATCGCGCA GTCCCACCGG 
GACATGGAGC AGGAGCTCGC CCACGCCGTC AACGCCAGCT CCAAGGCCAT GGACCGCGTG 
TACAACAAGC CCAGGACCGC TGAGGGGCTG ACCGGCAGCG GTGTTCTGGA CATGGTCAAC 
AACTTCAGGG CTCTGCGCAG CGAGACGGAG CTGCTGCTGG CCGGGAGGGT GGCCCTGCAG 
CTGGACCCCC CTCAGAAGGA GCGGCTGGGG GCCGCTCTCG CCGAGATGGA CCAGCAGCTC 
AGGAAGCTGG CAGATGCTCC CTGGCTGTGC CAGCCCACGG AGCCCAGTGA CGAAGAGAAC 
GTGACACTGG ATCTTTCCAA ACGCAGCCGC AGCGGGAAAT TCCGCCTGGT GACCAAGTTC 
AAGAAGGAGA AAAACAACAA GAACAGAGAA GCTCACGGCA GCCTGGGAGC CCCGGCTCAT 
CTTTGGGGCA CGGAGGAGGT TGCCGCCTGG CTGGAGCACC TCAGTCTCTG TGAGTATAAG 
GACATCTTCA CACGGCACGA CATCCGGGGC TCCGAGCTCC TGCACCTGGA GCGGAGGGAC 
CTCAAGGACC TCGGCGTGAC CAAGGTGGGT CACATGAAGA GGATCTTGTG TGGCATCAAG 
GAGCTGAGCC GGAGCCCCCC AGCCGCCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011379178.1
CDS	1..3516
Translation	MSPFLWFQTVIKEGVLSKQNSSFQRSRRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTACKESLLTRCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYETKPPRPASSSTVVTEDFSEGSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTAEGSEESEAVAKKCSVLKEKLDSLLKTLDDESQALSSLPTPPPTIAEEAEDGDGLGSGCGSAVDRSVGSACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQERLVLGLSESEGKKDLKSDDRLCHSGSCGAAKCRSLRKVSRSPCEKLISKGSLSLGSSASLPPQSGNRDGLPALNTKILFPNVRAGMSGSLPGGSVISRLLINADPFNSEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIIHKNRAQMLTRDRAFESTLKSWEDKQKCELPRPPSFSLHAETLSEEEATQMEQFGQAAGALIHSIREIAQSHRDMEQELAHAVNASSKAMDRVYNKPRTAEGLTGSGVLDMVNNFRALRSETELLLAGRVALQLDPPQKERLGAALAEMDQQLRKLADAPWLCQPTEPSDEENVTLDLSKRSRSGKFRLVTKFKKEKNNKNREAHGSLGAPAHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSPPAAEA

Target ORF information:

RefSeq Version	XM_011380876.1
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus diacylglycerol kinase, delta 130kDa (DGKD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011380876.1

ORF Insert Sequence:

ATGAGCCCGT TCCTGTGGTT TCAGACCGTC ATCAAAGAGG GGGTGCTGAG CAAACAGAAC 
AGCTCATTCC AGCGATCCAG AAGGAGGTAC TTCAAGCTTC GCGGGCGAAC GCTGTACTAT 
GCGAAGACGG CAAAGTCGAT CATATTTGAC GAGGTGGACC TGACAGATGC CAGCGTGGCT 
GAATCCAGCA CGAAAAACGT CAACAACAGT TTTACGGTCA TCACTCCATG CAGGAAGCTC 
ATCTTATGCG CCGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCTCT GAAGACTGTC 
CAGAACAGGG AGCATTTTGA GCCGACGCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AACTGGTACG CCTGCTCGCA CGCGAGGCCC ACCTACTGCA ACGTGTGCCG CGAGGCGCTG 
TCTGGGGTCA CGTCGCACGG CCTGTCCTGT GAAGTGTGCA AGTTTAAGGC CCACAAGCGG 
TGTGCTGTGC GTGCAACCAG TAACTGCAAG TGGACCACAC TGGCCTCCAT TGGGAAGGAC 
ATCATCGAGG ACGAGGATGG GGTCGCCATG CCTCACCAGT GGCTGGAAGG AAACCTGCCT 
GTGAGCGCCA AGTGCACAGT GTGCGACAAG ACCTGCGGCA GCGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTG CACACGGCGT GTAAGGAATC CTTGCTGACC 
AGGTGCCCGC TGGGCCTGTG TAAAGTGTCA GTCATCCCTC CCACGGCTCT CAACAGCATC 
GACTCCGATG GCTTCTGGAA GGCCACATGT CCTCCATCCT GCACAAGCCC CTTATTGGTC 
TTTGTCAATT CAAAAAGTGG GGACAACCAG GGCGTGAAGT TCCTCAGAAG GTTCAAGCAG 
CTGCTGAATC CTGCCCAGGT CTTCGACCTC ATGAATGGAG GCCCACACCT CGGCTTACGC 
TTATTCCAGA AGTTTGATAC CTTTCGGATT CTGGTGTGCG GTGGGGACGG CAGTGTCGGC 
TGGGTCCTCT CTGAAATTGA CAGCCTCAAT CTGCACAAAC AGTGTCAGCT GGGAGTGTTG 
CCGCTCGGCA CAGGGAACGA CCTGGCTCGT GTGTTAGGCT GGGGCTCGGC CTGTGACGAT 
GACACCCAGC TCCCGCAGAT CCTGGAGAAG CTGGAAAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCCTA TGAGACCAAA CCCCCCCGGC CGGCCTCCTC TTCCACCGTC 
GTCACCGAGG ACTTCAGCGA GGGTTCCGAG GTACAGCAGA TTCTCTTCTA TGAAGACTCT 
GTCGCGGCCC ATCTTTCTAA AATCCTGACC TCTGACCAGC ACTCGGTGGT GATATCATCG 
GCGAAGGTGC TCTGCGAGAC GGTGAAGGAC TTCGTGGCGC GAGTGGGCAA GGCCTATGAG 
AAGACAGCTG AGGGCTCGGA GGAGTCAGAG GCCGTGGCCA AGAAGTGCTC TGTCCTGAAA 
GAGAAGCTGG ATTCCCTCCT CAAGACTTTG GACGACGAGT CGCAGGCCTT GTCCTCTCTG 
CCCACCCCAC CGCCCACCAT TGCTGAGGAG GCGGAAGACG GAGACGGGCT GGGCAGCGGC 
TGTGGCTCCG CAGTGGACCG CTCGGTGGGG TCGGCGTGCC CAGCCCGGCC ACAGATATTC 
CGGCCTCGGG AACAACTCAT GCTAAGAGCC AACAGCCTGA AGAAGGCGAT CCGTCAGATC 
ATAGAACACA CAGAAAAAGC TGTCGACGAG CAGAACGCCC AGACCCAAGA ACAGGAGAGG 
CTTGTCTTGG GGCTCTCCGA GTCGGAGGGG AAGAAAGACC TGAAATCGGA TGACAGACTG 
TGCCACAGCG GGAGCTGCGG GGCGGCCAAG TGCAGGAGCC TCCGCAAAGT GTCCAGATCA 
CCATGTGAGA AGCTGATAAG CAAAGGAAGT TTGTCACTGG GCAGTTCTGC GTCTCTTCCT 
CCCCAGTCAG GAAATCGGGA CGGCCTGCCA GCACTGAACA CAAAGATCCT GTTTCCAAAC 
GTTCGGGCTG GGATGTCCGG TTCCTTGCCT GGAGGATCCG TCATCAGTCG CTTATTGATT 
AATGCTGATC CCTTCAACTC CGAACCAGAA AACCTAGAGT ACTACACAGA GAAGTGTGTC 
ATGAACAATT ACTTTGGCAT CGGCCTGGAT GCGAAGATAT CCCTGGACTT CAACAACAAG 
CGTGACGAAC ACCCGGAGAA GTGCAGGAGT CGAACCAAGA ACATGATGTG GTACGGGGTT 
CTCGGAACCA AGGAGCTGCT GCACAGAACC TACAAGAACC TGGAGCAGAA GGTCCTGCTG 
GAGTGTGATG GGCGGCCCAT CCCACTCCCC AGTCTCCAGG GAATTGCTGT CCTCAACATT 
CCCAGCTATG CCGGAGGGAC CAACTTCTGG GGGGGCACCA AAGAAGACGA TACTTTTGCA 
GCTCCCTCGT TCGATGATAA GATCTTGGAG GTGGTGGCCG TGTTTGGCAG TATGCAGATG 
GCTGTCTCTC GGGTCATTAA GCTGCAGCAT CACCGGATTG CCCAGTGTCG CACAGTGAAG 
ATTTCCATCC TGGGGGACGA GGGCGTGCCT GTGCAGGTGG ACGGAGAGGC CTGGATCCAG 
CCCCCAGGAT ACATTCGGAT CATTCACAAG AACCGGGCGC AGATGCTCAC CAGAGACAGG 
GCATTCGAGA GCACCCTGAA GTCCTGGGAG GACAAGCAGA AGTGCGAGCT GCCCCGCCCG 
CCGTCCTTCT CGCTGCACGC GGAGACCCTG TCCGAGGAGG AGGCCACACA GATGGAGCAG 
TTCGGGCAGG CGGCGGGAGC CCTCATCCAC AGCATCCGGG AGATCGCGCA GTCCCACCGG 
GACATGGAGC AGGAGCTCGC CCACGCCGTC AACGCCAGCT CCAAGGCCAT GGACCGCGTG 
TACAACAAGC CCAGGACCGC TGAGGGGCTG ACCGGCAGCG GTGTTCTGGA CATGGTCAAC 
AACTTCAGGG CTCTGCGCAG CGAGACGGAG CTGCTGCTGG CCGGGAGGGT GGCCCTGCAG 
CTGGACCCCC CTCAGAAGGA GCGGCTGGGG GCCGCTCTCG CCGAGATGGA CCAGCAGCTC 
AGGAAGCTGG CAGATGCTCC CTGGCTGTGC CAGCCCACGG AGCCCAGTGA CGAAGAGAAC 
GTGACACTGG ATCTTTCCAA ACGCAGCCGC AGCGGGAAAT TCCGCCTGGT GACCAAGTTC 
AAGAAGGAGA AAAACAACAA GAACAGAGAA GCTCACGGCA GCCTGGGAGC CCCGGCTCAT 
CTTTGGGGCA CGGAGGAGGT TGCCGCCTGG CTGGAGCACC TCAGTCTCTG TGAGTATAAG 
GACATCTTCA CACGGCACGA CATCCGGGGC TCCGAGCTCC TGCACCTGGA GCGGAGGGAC 
CTCAAGGACC TCGGCGTGAC CAAGGTGGGT CACATGAAGA GGATCTTGTG TGGCATCAAG 
GAGCTGAGCC GGAGCCCCCC AGCCGCCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKD ( XM_011380876.2 ) cDNA ORF clone, Pteropus vampyrus(large flying fox) -> XP_011379178.1 Pteropus vampyrus diacylglycerol kinase delta (DGKD), mRNA.

Price & Availability↑

CloneID	OPt61710
Clone ID Related Accession (Same CDS sequence)	XM_011380876.1 , XM_011380876.2
Accession Version	XM_011380876.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-18
Organism	Pteropus vampyrus(large flying fox)
Product	diacylglycerol kinase delta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011889192.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 19, 2018 this sequence version replaced XM_011380876.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus vampyrus Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCCGT TCCTGTGGTT TCAGACCGTC ATCAAAGAGG GGGTGCTGAG CAAACAGAAC 
AGCTCATTCC AGCGATCCAG AAGGAGGTAC TTCAAGCTTC GCGGGCGAAC GCTGTACTAT 
GCGAAGACGG CAAAGTCGAT CATATTTGAC GAGGTGGACC TGACAGATGC CAGCGTGGCT 
GAATCCAGCA CGAAAAACGT CAACAACAGT TTTACGGTCA TCACTCCATG CAGGAAGCTC 
ATCTTATGCG CCGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCTCT GAAGACTGTC 
CAGAACAGGG AGCATTTTGA GCCGACGCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AACTGGTACG CCTGCTCGCA CGCGAGGCCC ACCTACTGCA ACGTGTGCCG CGAGGCGCTG 
TCTGGGGTCA CGTCGCACGG CCTGTCCTGT GAAGTGTGCA AGTTTAAGGC CCACAAGCGG 
TGTGCTGTGC GTGCAACCAG TAACTGCAAG TGGACCACAC TGGCCTCCAT TGGGAAGGAC 
ATCATCGAGG ACGAGGATGG GGTCGCCATG CCTCACCAGT GGCTGGAAGG AAACCTGCCT 
GTGAGCGCCA AGTGCACAGT GTGCGACAAG ACCTGCGGCA GCGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTG CACACGGCGT GTAAGGAATC CTTGCTGACC 
AGGTGCCCGC TGGGCCTGTG TAAAGTGTCA GTCATCCCTC CCACGGCTCT CAACAGCATC 
GACTCCGATG GCTTCTGGAA GGCCACATGT CCTCCATCCT GCACAAGCCC CTTATTGGTC 
TTTGTCAATT CAAAAAGTGG GGACAACCAG GGCGTGAAGT TCCTCAGAAG GTTCAAGCAG 
CTGCTGAATC CTGCCCAGGT CTTCGACCTC ATGAATGGAG GCCCACACCT CGGCTTACGC 
TTATTCCAGA AGTTTGATAC CTTTCGGATT CTGGTGTGCG GTGGGGACGG CAGTGTCGGC 
TGGGTCCTCT CTGAAATTGA CAGCCTCAAT CTGCACAAAC AGTGTCAGCT GGGAGTGTTG 
CCGCTCGGCA CAGGGAACGA CCTGGCTCGT GTGTTAGGCT GGGGCTCGGC CTGTGACGAT 
GACACCCAGC TCCCGCAGAT CCTGGAGAAG CTGGAAAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCCTA TGAGACCAAA CCCCCCCGGC CGGCCTCCTC TTCCACCGTC 
GTCACCGAGG ACTTCAGCGA GGGTTCCGAG GTACAGCAGA TTCTCTTCTA TGAAGACTCT 
GTCGCGGCCC ATCTTTCTAA AATCCTGACC TCTGACCAGC ACTCGGTGGT GATATCATCG 
GCGAAGGTGC TCTGCGAGAC GGTGAAGGAC TTCGTGGCGC GAGTGGGCAA GGCCTATGAG 
AAGACAGCTG AGGGCTCGGA GGAGTCAGAG GCCGTGGCCA AGAAGTGCTC TGTCCTGAAA 
GAGAAGCTGG ATTCCCTCCT CAAGACTTTG GACGACGAGT CGCAGGCCTT GTCCTCTCTG 
CCCACCCCAC CGCCCACCAT TGCTGAGGAG GCGGAAGACG GAGACGGGCT GGGCAGCGGC 
TGTGGCTCCG CAGTGGACCG CTCGGTGGGG TCGGCGTGCC CAGCCCGGCC ACAGATATTC 
CGGCCTCGGG AACAACTCAT GCTAAGAGCC AACAGCCTGA AGAAGGCGAT CCGTCAGATC 
ATAGAACACA CAGAAAAAGC TGTCGACGAG CAGAACGCCC AGACCCAAGA ACAGGAGAGG 
CTTGTCTTGG GGCTCTCCGA GTCGGAGGGG AAGAAAGACC TGAAATCGGA TGACAGACTG 
TGCCACAGCG GGAGCTGCGG GGCGGCCAAG TGCAGGAGCC TCCGCAAAGT GTCCAGATCA 
CCATGTGAGA AGCTGATAAG CAAAGGAAGT TTGTCACTGG GCAGTTCTGC GTCTCTTCCT 
CCCCAGTCAG GAAATCGGGA CGGCCTGCCA GCACTGAACA CAAAGATCCT GTTTCCAAAC 
GTTCGGGCTG GGATGTCCGG TTCCTTGCCT GGAGGATCCG TCATCAGTCG CTTATTGATT 
AATGCTGATC CCTTCAACTC CGAACCAGAA AACCTAGAGT ACTACACAGA GAAGTGTGTC 
ATGAACAATT ACTTTGGCAT CGGCCTGGAT GCGAAGATAT CCCTGGACTT CAACAACAAG 
CGTGACGAAC ACCCGGAGAA GTGCAGGAGT CGAACCAAGA ACATGATGTG GTACGGGGTT 
CTCGGAACCA AGGAGCTGCT GCACAGAACC TACAAGAACC TGGAGCAGAA GGTCCTGCTG 
GAGTGTGATG GGCGGCCCAT CCCACTCCCC AGTCTCCAGG GAATTGCTGT CCTCAACATT 
CCCAGCTATG CCGGAGGGAC CAACTTCTGG GGGGGCACCA AAGAAGACGA TACTTTTGCA 
GCTCCCTCGT TCGATGATAA GATCTTGGAG GTGGTGGCCG TGTTTGGCAG TATGCAGATG 
GCTGTCTCTC GGGTCATTAA GCTGCAGCAT CACCGGATTG CCCAGTGTCG CACAGTGAAG 
ATTTCCATCC TGGGGGACGA GGGCGTGCCT GTGCAGGTGG ACGGAGAGGC CTGGATCCAG 
CCCCCAGGAT ACATTCGGAT CATTCACAAG AACCGGGCGC AGATGCTCAC CAGAGACAGG 
GCATTCGAGA GCACCCTGAA GTCCTGGGAG GACAAGCAGA AGTGCGAGCT GCCCCGCCCG 
CCGTCCTTCT CGCTGCACGC GGAGACCCTG TCCGAGGAGG AGGCCACACA GATGGAGCAG 
TTCGGGCAGG CGGCGGGAGC CCTCATCCAC AGCATCCGGG AGATCGCGCA GTCCCACCGG 
GACATGGAGC AGGAGCTCGC CCACGCCGTC AACGCCAGCT CCAAGGCCAT GGACCGCGTG 
TACAACAAGC CCAGGACCGC TGAGGGGCTG ACCGGCAGCG GTGTTCTGGA CATGGTCAAC 
AACTTCAGGG CTCTGCGCAG CGAGACGGAG CTGCTGCTGG CCGGGAGGGT GGCCCTGCAG 
CTGGACCCCC CTCAGAAGGA GCGGCTGGGG GCCGCTCTCG CCGAGATGGA CCAGCAGCTC 
AGGAAGCTGG CAGATGCTCC CTGGCTGTGC CAGCCCACGG AGCCCAGTGA CGAAGAGAAC 
GTGACACTGG ATCTTTCCAA ACGCAGCCGC AGCGGGAAAT TCCGCCTGGT GACCAAGTTC 
AAGAAGGAGA AAAACAACAA GAACAGAGAA GCTCACGGCA GCCTGGGAGC CCCGGCTCAT 
CTTTGGGGCA CGGAGGAGGT TGCCGCCTGG CTGGAGCACC TCAGTCTCTG TGAGTATAAG 
GACATCTTCA CACGGCACGA CATCCGGGGC TCCGAGCTCC TGCACCTGGA GCGGAGGGAC 
CTCAAGGACC TCGGCGTGAC CAAGGTGGGT CACATGAAGA GGATCTTGTG TGGCATCAAG 
GAGCTGAGCC GGAGCCCCCC AGCCGCCGAG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011379178.1
CDS	1..3516
Translation	MSPFLWFQTVIKEGVLSKQNSSFQRSRRRYFKLRGRTLYYAKTAKSIIFDEVDLTDASVAESSTKNVNNSFTVITPCRKLILCADNRKEMEDWIAALKTVQNREHFEPTQYSMDHFSGMHNWYACSHARPTYCNVCREALSGVTSHGLSCEVCKFKAHKRCAVRATSNCKWTTLASIGKDIIEDEDGVAMPHQWLEGNLPVSAKCTVCDKTCGSVLRLQDWRCLWCKAMVHTACKESLLTRCPLGLCKVSVIPPTALNSIDSDGFWKATCPPSCTSPLLVFVNSKSGDNQGVKFLRRFKQLLNPAQVFDLMNGGPHLGLRLFQKFDTFRILVCGGDGSVGWVLSEIDSLNLHKQCQLGVLPLGTGNDLARVLGWGSACDDDTQLPQILEKLERASTKMLDRWSVMAYETKPPRPASSSTVVTEDFSEGSEVQQILFYEDSVAAHLSKILTSDQHSVVISSAKVLCETVKDFVARVGKAYEKTAEGSEESEAVAKKCSVLKEKLDSLLKTLDDESQALSSLPTPPPTIAEEAEDGDGLGSGCGSAVDRSVGSACPARPQIFRPREQLMLRANSLKKAIRQIIEHTEKAVDEQNAQTQEQERLVLGLSESEGKKDLKSDDRLCHSGSCGAAKCRSLRKVSRSPCEKLISKGSLSLGSSASLPPQSGNRDGLPALNTKILFPNVRAGMSGSLPGGSVISRLLINADPFNSEPENLEYYTEKCVMNNYFGIGLDAKISLDFNNKRDEHPEKCRSRTKNMMWYGVLGTKELLHRTYKNLEQKVLLECDGRPIPLPSLQGIAVLNIPSYAGGTNFWGGTKEDDTFAAPSFDDKILEVVAVFGSMQMAVSRVIKLQHHRIAQCRTVKISILGDEGVPVQVDGEAWIQPPGYIRIIHKNRAQMLTRDRAFESTLKSWEDKQKCELPRPPSFSLHAETLSEEEATQMEQFGQAAGALIHSIREIAQSHRDMEQELAHAVNASSKAMDRVYNKPRTAEGLTGSGVLDMVNNFRALRSETELLLAGRVALQLDPPQKERLGAALAEMDQQLRKLADAPWLCQPTEPSDEENVTLDLSKRSRSGKFRLVTKFKKEKNNKNREAHGSLGAPAHLWGTEEVAAWLEHLSLCEYKDIFTRHDIRGSELLHLERRDLKDLGVTKVGHMKRILCGIKELSRSPPAAEA

Target ORF information:

RefSeq Version	XM_011380876.2
Organism	Pteropus vampyrus(large flying fox)
Definition	Pteropus vampyrus diacylglycerol kinase delta (DGKD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011380876.2

ORF Insert Sequence:

ATGAGCCCGT TCCTGTGGTT TCAGACCGTC ATCAAAGAGG GGGTGCTGAG CAAACAGAAC 
AGCTCATTCC AGCGATCCAG AAGGAGGTAC TTCAAGCTTC GCGGGCGAAC GCTGTACTAT 
GCGAAGACGG CAAAGTCGAT CATATTTGAC GAGGTGGACC TGACAGATGC CAGCGTGGCT 
GAATCCAGCA CGAAAAACGT CAACAACAGT TTTACGGTCA TCACTCCATG CAGGAAGCTC 
ATCTTATGCG CCGATAACAG AAAAGAAATG GAAGATTGGA TTGCAGCTCT GAAGACTGTC 
CAGAACAGGG AGCATTTTGA GCCGACGCAG TACAGCATGG ACCACTTCTC AGGGATGCAC 
AACTGGTACG CCTGCTCGCA CGCGAGGCCC ACCTACTGCA ACGTGTGCCG CGAGGCGCTG 
TCTGGGGTCA CGTCGCACGG CCTGTCCTGT GAAGTGTGCA AGTTTAAGGC CCACAAGCGG 
TGTGCTGTGC GTGCAACCAG TAACTGCAAG TGGACCACAC TGGCCTCCAT TGGGAAGGAC 
ATCATCGAGG ACGAGGATGG GGTCGCCATG CCTCACCAGT GGCTGGAAGG AAACCTGCCT 
GTGAGCGCCA AGTGCACAGT GTGCGACAAG ACCTGCGGCA GCGTGCTGCG CCTGCAGGAC 
TGGCGCTGCC TCTGGTGCAA GGCCATGGTG CACACGGCGT GTAAGGAATC CTTGCTGACC 
AGGTGCCCGC TGGGCCTGTG TAAAGTGTCA GTCATCCCTC CCACGGCTCT CAACAGCATC 
GACTCCGATG GCTTCTGGAA GGCCACATGT CCTCCATCCT GCACAAGCCC CTTATTGGTC 
TTTGTCAATT CAAAAAGTGG GGACAACCAG GGCGTGAAGT TCCTCAGAAG GTTCAAGCAG 
CTGCTGAATC CTGCCCAGGT CTTCGACCTC ATGAATGGAG GCCCACACCT CGGCTTACGC 
TTATTCCAGA AGTTTGATAC CTTTCGGATT CTGGTGTGCG GTGGGGACGG CAGTGTCGGC 
TGGGTCCTCT CTGAAATTGA CAGCCTCAAT CTGCACAAAC AGTGTCAGCT GGGAGTGTTG 
CCGCTCGGCA CAGGGAACGA CCTGGCTCGT GTGTTAGGCT GGGGCTCGGC CTGTGACGAT 
GACACCCAGC TCCCGCAGAT CCTGGAGAAG CTGGAAAGAG CCAGCACCAA GATGCTGGAC 
AGGTGGAGCG TCATGGCCTA TGAGACCAAA CCCCCCCGGC CGGCCTCCTC TTCCACCGTC 
GTCACCGAGG ACTTCAGCGA GGGTTCCGAG GTACAGCAGA TTCTCTTCTA TGAAGACTCT 
GTCGCGGCCC ATCTTTCTAA AATCCTGACC TCTGACCAGC ACTCGGTGGT GATATCATCG 
GCGAAGGTGC TCTGCGAGAC GGTGAAGGAC TTCGTGGCGC GAGTGGGCAA GGCCTATGAG 
AAGACAGCTG AGGGCTCGGA GGAGTCAGAG GCCGTGGCCA AGAAGTGCTC TGTCCTGAAA 
GAGAAGCTGG ATTCCCTCCT CAAGACTTTG GACGACGAGT CGCAGGCCTT GTCCTCTCTG 
CCCACCCCAC CGCCCACCAT TGCTGAGGAG GCGGAAGACG GAGACGGGCT GGGCAGCGGC 
TGTGGCTCCG CAGTGGACCG CTCGGTGGGG TCGGCGTGCC CAGCCCGGCC ACAGATATTC 
CGGCCTCGGG AACAACTCAT GCTAAGAGCC AACAGCCTGA AGAAGGCGAT CCGTCAGATC 
ATAGAACACA CAGAAAAAGC TGTCGACGAG CAGAACGCCC AGACCCAAGA ACAGGAGAGG 
CTTGTCTTGG GGCTCTCCGA GTCGGAGGGG AAGAAAGACC TGAAATCGGA TGACAGACTG 
TGCCACAGCG GGAGCTGCGG GGCGGCCAAG TGCAGGAGCC TCCGCAAAGT GTCCAGATCA 
CCATGTGAGA AGCTGATAAG CAAAGGAAGT TTGTCACTGG GCAGTTCTGC GTCTCTTCCT 
CCCCAGTCAG GAAATCGGGA CGGCCTGCCA GCACTGAACA CAAAGATCCT GTTTCCAAAC 
GTTCGGGCTG GGATGTCCGG TTCCTTGCCT GGAGGATCCG TCATCAGTCG CTTATTGATT 
AATGCTGATC CCTTCAACTC CGAACCAGAA AACCTAGAGT ACTACACAGA GAAGTGTGTC 
ATGAACAATT ACTTTGGCAT CGGCCTGGAT GCGAAGATAT CCCTGGACTT CAACAACAAG 
CGTGACGAAC ACCCGGAGAA GTGCAGGAGT CGAACCAAGA ACATGATGTG GTACGGGGTT 
CTCGGAACCA AGGAGCTGCT GCACAGAACC TACAAGAACC TGGAGCAGAA GGTCCTGCTG 
GAGTGTGATG GGCGGCCCAT CCCACTCCCC AGTCTCCAGG GAATTGCTGT CCTCAACATT 
CCCAGCTATG CCGGAGGGAC CAACTTCTGG GGGGGCACCA AAGAAGACGA TACTTTTGCA 
GCTCCCTCGT TCGATGATAA GATCTTGGAG GTGGTGGCCG TGTTTGGCAG TATGCAGATG 
GCTGTCTCTC GGGTCATTAA GCTGCAGCAT CACCGGATTG CCCAGTGTCG CACAGTGAAG 
ATTTCCATCC TGGGGGACGA GGGCGTGCCT GTGCAGGTGG ACGGAGAGGC CTGGATCCAG 
CCCCCAGGAT ACATTCGGAT CATTCACAAG AACCGGGCGC AGATGCTCAC CAGAGACAGG 
GCATTCGAGA GCACCCTGAA GTCCTGGGAG GACAAGCAGA AGTGCGAGCT GCCCCGCCCG 
CCGTCCTTCT CGCTGCACGC GGAGACCCTG TCCGAGGAGG AGGCCACACA GATGGAGCAG 
TTCGGGCAGG CGGCGGGAGC CCTCATCCAC AGCATCCGGG AGATCGCGCA GTCCCACCGG 
GACATGGAGC AGGAGCTCGC CCACGCCGTC AACGCCAGCT CCAAGGCCAT GGACCGCGTG 
TACAACAAGC CCAGGACCGC TGAGGGGCTG ACCGGCAGCG GTGTTCTGGA CATGGTCAAC 
AACTTCAGGG CTCTGCGCAG CGAGACGGAG CTGCTGCTGG CCGGGAGGGT GGCCCTGCAG 
CTGGACCCCC CTCAGAAGGA GCGGCTGGGG GCCGCTCTCG CCGAGATGGA CCAGCAGCTC 
AGGAAGCTGG CAGATGCTCC CTGGCTGTGC CAGCCCACGG AGCCCAGTGA CGAAGAGAAC 
GTGACACTGG ATCTTTCCAA ACGCAGCCGC AGCGGGAAAT TCCGCCTGGT GACCAAGTTC 
AAGAAGGAGA AAAACAACAA GAACAGAGAA GCTCACGGCA GCCTGGGAGC CCCGGCTCAT 
CTTTGGGGCA CGGAGGAGGT TGCCGCCTGG CTGGAGCACC TCAGTCTCTG TGAGTATAAG 
GACATCTTCA CACGGCACGA CATCCGGGGC TCCGAGCTCC TGCACCTGGA GCGGAGGGAC 
CTCAAGGACC TCGGCGTGAC CAAGGTGGGT CACATGAAGA GGATCTTGTG TGGCATCAAG 
GAGCTGAGCC GGAGCCCCCC AGCCGCCGAG GCCTAG