NAE1 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	NAE1
Entrez Gene ID	102891210
Full Name	NEDD8 activating enzyme E1 subunit 1
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006909733.3	XP_006909795.1	NEDD8-activating enzyme E1 regulatory subunit
XM_006909733.2	XP_006909795.1	NEDD8-activating enzyme E1 regulatory subunit

Pathways from BioSystems
Gene Ontology

The following NAE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NAE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt12135	XM_006909733.3 Latest version!	Pteropus alecto NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OPt12135	XM_006909733.2	Pteropus alecto NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NAE1 ( XM_006909733.3 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006909795.1 Pteropus alecto NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Price & Availability↑

CloneID	OPt12135
Clone ID Related Accession (Same CDS sequence)	XM_006909733.3 , XM_006909733.2
Accession Version	XM_006909733.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	NEDD8-activating enzyme E1 regulatory subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434825.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006909733.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAC CCGGGAAGCT GCTCAAGGAG CAGAAATACG ACCGGCAGCT GAGGTTATGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA TTACCAGGTA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTCCA AAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTAGT TGCAACTCAG CTTCCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGACCTAC GTCTGGATAA GCCATTTCCT GAACTGAGAG AACATTTCCA GTCTTATGAT 
TTGGATCATA TGGATAAAAA GGACCATAGT CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TATTTAGCAC ACTGGTATAG TGAAACAAAT GGACGAATGC CTAAGACGTA TAAAGAAAAA 
GAAGAGTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAGA ATGAAAACGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGGA GTATCGAAGA TATTTTCAAT GATGATCGCT GCACAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTCCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATACATA 
AAACTGCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT 
GTTGCAAAAT TGCTGCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGCT TGGATACAAT TAACAAGGAT GAAATTACTT CCAGCATGGA CAGTCCAGAT 
AATGAGATAG TATTATACTT AATGTTACGG GCTGTGGATA GATTTCATAA ACAACATGGT 
AGATATCCGG GGGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGACTA TCTGTAATGG TGAAAGATGA TTATGTCCAT 
GAATTTTGCC GATACGGAGC TGCTGAGCCA CACACCATTG CTGCATTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006909795.1
CDS	49..1653
Translation	MAQPGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAQAAMEFLQELNNDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLPESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMDKKDHSHTPWIVIVAKYLAHWYSETNGRMPKTYKEKEEFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPRSIEDIFNDDRCTNITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEITSSMDSPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_006909733.3
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006909733.3

ORF Insert Sequence:

ATGGCGCAAC CCGGGAAGCT GCTCAAGGAG CAGAAATACG ACCGGCAGCT GAGGTTATGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA TTACCAGGTA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTCCA AAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTAGT TGCAACTCAG CTTCCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGACCTAC GTCTGGATAA GCCATTTCCT GAACTGAGAG AACATTTCCA GTCTTATGAT 
TTGGATCATA TGGATAAAAA GGACCATAGT CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TATTTAGCAC ACTGGTATAG TGAAACAAAT GGACGAATGC CTAAGACGTA TAAAGAAAAA 
GAAGAGTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAGA ATGAAAACGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGGA GTATCGAAGA TATTTTCAAT GATGATCGCT GCACAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTCCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATACATA 
AAACTGCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT 
GTTGCAAAAT TGCTGCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGCT TGGATACAAT TAACAAGGAT GAAATTACTT CCAGCATGGA CAGTCCAGAT 
AATGAGATAG TATTATACTT AATGTTACGG GCTGTGGATA GATTTCATAA ACAACATGGT 
AGATATCCGG GGGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGACTA TCTGTAATGG TGAAAGATGA TTATGTCCAT 
GAATTTTGCC GATACGGAGC TGCTGAGCCA CACACCATTG CTGCATTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NAE1 ( XM_006909733.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006909795.1 Pteropus alecto NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Price & Availability↑

CloneID	OPt12135
Clone ID Related Accession (Same CDS sequence)	XM_006909733.3 , XM_006909733.2
Accession Version	XM_006909733.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	NEDD8-activating enzyme E1 regulatory subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006434825.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006909733.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAAC CCGGGAAGCT GCTCAAGGAG CAGAAATACG ACCGGCAGCT GAGGTTATGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA TTACCAGGTA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTCCA AAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTAGT TGCAACTCAG CTTCCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGACCTAC GTCTGGATAA GCCATTTCCT GAACTGAGAG AACATTTCCA GTCTTATGAT 
TTGGATCATA TGGATAAAAA GGACCATAGT CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TATTTAGCAC ACTGGTATAG TGAAACAAAT GGACGAATGC CTAAGACGTA TAAAGAAAAA 
GAAGAGTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAGA ATGAAAACGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGGA GTATCGAAGA TATTTTCAAT GATGATCGCT GCACAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTCCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATACATA 
AAACTGCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT 
GTTGCAAAAT TGCTGCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGCT TGGATACAAT TAACAAGGAT GAAATTACTT CCAGCATGGA CAGTCCAGAT 
AATGAGATAG TATTATACTT AATGTTACGG GCTGTGGATA GATTTCATAA ACAACATGGT 
AGATATCCGG GGGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGACTA TCTGTAATGG TGAAAGATGA TTATGTCCAT 
GAATTTTGCC GATACGGAGC TGCTGAGCCA CACACCATTG CTGCATTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006909795.1
CDS	29..1633
Translation	MAQPGKLLKEQKYDRQLRLWGDHGQEALESAHVCLINATATGTEILKNLVLPGIGSFTIIDGNQVSGEDAGNNFFLQRSSIGKNRAQAAMEFLQELNNDVSGSFVEESPENLLDNDPSFFCRFTIVVATQLPESTLLRLADVLWNSQIPLLICRTYGLVGYMRIIIKEHPVIESHPDNALEDLRLDKPFPELREHFQSYDLDHMDKKDHSHTPWIVIVAKYLAHWYSETNGRMPKTYKEKEEFRDLIRQGILKNENGAPEDEENFEEAIKNVNTALNTTQIPRSIEDIFNDDRCTNITKQTPSFWILARALKEFVAKEGQGNLPVRGTIPDMIADSGKYIKLQNVYREKAKKDAAAVGNHVAKLLQSIGQAPESISEKELKLLCSNSAFLRVVRCRSLAEEYGLDTINKDEITSSMDSPDNEIVLYLMLRAVDRFHKQHGRYPGVSNYQVEEDIGKLKSCLTGFLQEYGLSVMVKDDYVHEFCRYGAAEPHTIAAFLGGAAAQEVIKIITKQFVIFNNTYIYSGMSQTSATFQL

Target ORF information:

RefSeq Version	XM_006909733.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto NEDD8 activating enzyme E1 subunit 1 (NAE1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006909733.2

ORF Insert Sequence:

ATGGCGCAAC CCGGGAAGCT GCTCAAGGAG CAGAAATACG ACCGGCAGCT GAGGTTATGG 
GGTGATCATG GGCAAGAGGC TTTAGAATCT GCTCATGTTT GCCTAATAAA TGCAACAGCC 
ACAGGAACTG AAATTCTTAA AAACTTGGTA TTACCAGGTA TTGGTTCATT TACAATTATT 
GATGGAAATC AGGTCAGCGG AGAAGATGCT GGAAATAATT TTTTCCTCCA AAGAAGCAGT 
ATTGGCAAGA ACCGAGCTCA AGCTGCCATG GAATTCTTAC AAGAATTAAA TAACGATGTC 
TCTGGGAGTT TTGTGGAAGA GAGTCCAGAA AACCTTCTAG ACAATGATCC TTCATTTTTC 
TGTAGGTTTA CCATTGTAGT TGCAACTCAG CTTCCTGAAA GTACATTACT ACGTTTAGCA 
GATGTCCTCT GGAATTCCCA AATCCCTCTT TTGATCTGTA GGACATATGG ACTAGTTGGT 
TATATGAGGA TCATTATAAA AGAACATCCA GTAATAGAAT CTCATCCAGA TAATGCATTA 
GAGGACCTAC GTCTGGATAA GCCATTTCCT GAACTGAGAG AACATTTCCA GTCTTATGAT 
TTGGATCATA TGGATAAAAA GGACCATAGT CACACTCCAT GGATTGTGAT CGTAGCTAAA 
TATTTAGCAC ACTGGTATAG TGAAACAAAT GGACGAATGC CTAAGACGTA TAAAGAAAAA 
GAAGAGTTCA GAGATTTGAT TAGACAAGGA ATTCTAAAGA ATGAAAACGG GGCTCCAGAA 
GATGAAGAGA ATTTTGAAGA AGCTATTAAA AATGTGAACA CAGCACTAAA TACAACTCAG 
ATCCCAAGGA GTATCGAAGA TATTTTCAAT GATGATCGCT GCACAAATAT CACCAAACAG 
ACTCCATCAT TTTGGATTTT AGCTCGTGCC TTAAAGGAAT TTGTGGCCAA AGAGGGTCAA 
GGAAATTTAC CTGTCCGAGG CACAATTCCT GATATGATTG CAGATTCAGG CAAATACATA 
AAACTGCAAA ATGTTTACCG TGAAAAAGCA AAGAAAGATG CTGCTGCTGT GGGTAATCAT 
GTTGCAAAAT TGCTGCAGTC TATAGGCCAG GCACCAGAGT CCATTTCAGA GAAAGAATTA 
AAATTACTCT GCAGCAATTC TGCATTTCTT CGAGTGGTAA GATGTCGATC CTTAGCTGAA 
GAATATGGCT TGGATACAAT TAACAAGGAT GAAATTACTT CCAGCATGGA CAGTCCAGAT 
AATGAGATAG TATTATACTT AATGTTACGG GCTGTGGATA GATTTCATAA ACAACATGGT 
AGATATCCGG GGGTATCTAA CTATCAAGTT GAAGAAGATA TAGGAAAGTT GAAGTCTTGT 
CTCACTGGCT TCCTTCAGGA ATATGGACTA TCTGTAATGG TGAAAGATGA TTATGTCCAT 
GAATTTTGCC GATACGGAGC TGCTGAGCCA CACACCATTG CTGCATTCTT AGGGGGAGCT 
GCTGCTCAAG AGGTTATCAA AATAATCACC AAACAATTTG TAATTTTTAA TAATACTTAC 
ATTTATAGTG GCATGTCACA AACTTCAGCA ACTTTTCAGT TGTAA