DACT1 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	DACT1
Entrez Gene ID	102887779
Full Name	dishevelled binding antagonist of beta catenin 1
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015595820.1	XP_015451306.1	dapper homolog 1
XM_015595820.2	XP_015451306.1	dapper homolog 1

Pathways from BioSystems
Gene Ontology

The following DACT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DACT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt24526	XM_015595820.2 Latest version!	Pteropus alecto dishevelled binding antagonist of beta catenin 1 (DACT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OPt24526	XM_015595820.1	Pteropus alecto dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DACT1 ( XM_015595820.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015451306.1 Pteropus alecto dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.

Price & Availability↑

CloneID	OPt24526
Clone ID Related Accession (Same CDS sequence)	XM_015595820.1 , XM_015595820.2
Accession Version	XM_015595820.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	dapper homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006442109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGTTAAAGA CAGTACCTCA GTCATTTCTC TTCGCTGGTG CAGAAATGAT CCAGAAACCA 
TACTGGAAAT TGCTCTGCTT GCCTTTACGA AGTGGAGTCA AAAAATCCAA CCTCACAGAA 
GTTGTCTTTG AATTCACTCG CGGTACCTTG CTTGGTAAGC GCCAGGAGCT GCTGGTCAGG 
GGCGCCCTGC GCGGCGTTGG GGGCGCTGGG GCCGCTGTGC CGCGCGCCGG GGAGCTGCCG 
GGGGAGGCGG CACAGCGCAG CCGCCTGGAG GAGAAGTTCT TGGAGGAGAA CATCTTGCTG 
CTGCGGAAGC AATTGAATTG TTTGAGGAGA AGAGATGTTG GTTTGTTAAA CCAGTTGCAA 
GAACTTGACA AGCAGATAAG TGACCTAAGA CTTGATGTAG AAAAGACATC TGAAGAGCAC 
TTGGAGACAG ACAGCCGGCC CAGCTCAGGG TTTTATGAGC TGAGTGATGG GGCTTCAGGA 
TCTCTTTCCA ATTCTTCTAA CTCGGTCTTC AGTGAATGTT TGTCCAGTTG TCATTCCAGC 
ACCTGCTTTT GCAGCCCCTT GGAGGCAACC TTGACTATCT CAGATGGTTG CCCCAAATCT 
GCAGATCTCA TAGGATGGTT GGACTATAAA GAAGGCCACT GTGAAGACCA GGCCTCAGAG 
ACAGTTTGCT GTTCCCCCTC CACATCACAA TTTAATCCCC TTGATGTCAT TGCAGATGTG 
AATCCCAAGT ATCAGTGCGA TCTGGTGTCT AAAAACGGGA ATGATGTCTA TCGCTATCCC 
AGTCCGCTTC ATGCCGTGGC TGTGCAGAGC CCAATGTTTC TCCTCTGCCT GACGGGCAAC 
TCCCTGAGGG AAGAGGAGAG GTTGAGTAAC CATGCCAGTG ACATTTGTGT CCGATCTGAG 
CTGGACGGCA TCAAGACAGA CACCTCCTTA CCATCTCCAA GCAGTTTGTG GTCTGCTCCC 
CATCCTTCAT CCAGTAAGAA AATGGATGGC TACATTCTGA GCCTGGTCCA GAAAAAAACA 
CACCCTGTAA GGACCAACAA ACCAAGAACC AGCGTGAACG CTGACCCCAC AAAGGGGCTT 
CTGAGGAATG GGAGTGTTTG TGTCAGAGCG ACTGGAGGCA TCACACAAGG CAATAGTGGG 
AACCTCAAGA ATTCTAAACA GGCGTCTTTG CCCTCTGGTG GAATCCCCTC TCTGGACAAT 
GGGACATTCT CCCCACTGAA GCAGTGGTCG AAGGAATCAA AGGCAGAACA ACTGGAAAGC 
AAGAGGTTGC CCCTGCCTGA GGGCTGCCCC CCTGGAGCTG CCGCTGAACT TCAAAGCAAG 
CATCCGCCCA AAAATGGCAA GCCAGCCTCC CAGGAACATG CTCGGTGTCC CACCACTGTG 
ACAGGGGAGT CCCCTAAAGA AAGCAGTCAG ATCCCAGCTT CCTCACCAAA AGAGAGCCCT 
GGTAGAGCCC TCGTCCCACT GCAGGAAAAC AAAGTTGTCC AGCCACCACT GAAAAAGATG 
GCGCAGAAAA ACAGCCTGCA GGCCGTCCCT CCAGCGGTGC CCCCTCCTGC CCCTTCTGTA 
CTGCTGTCCT CAGCATTTCC GGTGGAAGAA AGGCCTGCCC TGGATTTCAA AAGCGAGGGC 
TCTTCTTCAC AAAGCCTGGA GGATGGGCCT CTGGTGAAGG CTCACTTCGT CCCAGGCCAG 
CAGCCTGGCG TCAGGCCGCA CCGGGGTACC CGGACCATGG CTGTCCCCAG GGGCTCCGCC 
CTGAAGCACA GGGTCCAGGC CCTCCACGGC CCGGACGGCG CCTTGCCCAC CGTGAGGGAG 
AAAACCCGGG CGCCCAGCAA GAAGTGTCGG TTCCCCGACG ACGTGGATAC AAATAAGAAA 
CTCAGGAAGG CCTCAGCCAA GGGGAGGAAG GGCGGGGGCG GCCACGCCGA GGCGGGTTTC 
CCCAGCCGGC CGCTGGGCGC TGCCCACCGG GCGGGGAGCA GGGTGCACGG CCACGGCCGG 
GAGGCCGTGG TGGCCAAGCC CAAGCACAAG CGAACTGACT CGCGGCGCTG GAGGTCGGCC 
GCCGAGCTGT CCTACGAAGA AGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGCGCG 
GGCCTGTACC CGGCCGCGGT GGCGCTGCCC TACGCCAGCC CCTACGCCTA CGTGGCCAGC 
GACTCCGAGT ACTCGGCCGA GTGCGAGTCC CTGTTCCACT CCACCGTAGT GGACACCAGC 
GAGGACGAGC AGAGCAACTA CACTACCAAC TGTTTCGGGG ACAGCGAGTC CAGTGTGAGC 
GAGGGCGAGT TTGGCGGGGA GAGCACGAGC ACCAGCGACT CGGAGGAAAG CGGGGGCCTA 
ATCTGGTCCC AGTTTGTCCA GACTCTCCCG ATTCAGACGG TCACAGCCCC AGACCTTCAC 
AACAACCCCA CGAAAACCTT TGTCAAAATT AAGGCTTCTC ATAACCTCAA GAAGAAAATC 
CTACGCTTTC GGTCTGGCTC TTTGAAGCTG ATGACGACTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015451306.1
CDS	1..2505
Translation	MLKTVPQSFLFAGAEMIQKPYWKLLCLPLRSGVKKSNLTEVVFEFTRGTLLGKRQELLVRGALRGVGGAGAAVPRAGELPGEAAQRSRLEEKFLEENILLLRKQLNCLRRRDVGLLNQLQELDKQISDLRLDVEKTSEEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEATLTISDGCPKSADLIGWLDYKEGHCEDQASETVCCSPSTSQFNPLDVIADVNPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNSLREEERLSNHASDICVRSELDGIKTDTSLPSPSSLWSAPHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRATGGITQGNSGNLKNSKQASLPSGGIPSLDNGTFSPLKQWSKESKAEQLESKRLPLPEGCPPGAAAELQSKHPPKNGKPASQEHARCPTTVTGESPKESSQIPASSPKESPGRALVPLQENKVVQPPLKKMAQKNSLQAVPPAVPPPAPSVLLSSAFPVEERPALDFKSEGSSSQSLEDGPLVKAHFVPGQQPGVRPHRGTRTMAVPRGSALKHRVQALHGPDGALPTVREKTRAPSKKCRFPDDVDTNKKLRKASAKGRKGGGGHAEAGFPSRPLGAAHRAGSRVHGHGREAVVAKPKHKRTDSRRWRSAAELSYEEnullnullnullnullnullnullnullnullnullnullnullnullAGLYPAAVALPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFGGESTSTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNNPTKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	XM_015595820.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto dishevelled-binding antagonist of beta-catenin 1 (DACT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015595820.1

ORF Insert Sequence:

ATGTTAAAGA CAGTACCTCA GTCATTTCTC TTCGCTGGTG CAGAAATGAT CCAGAAACCA 
TACTGGAAAT TGCTCTGCTT GCCTTTACGA AGTGGAGTCA AAAAATCCAA CCTCACAGAA 
GTTGTCTTTG AATTCACTCG CGGTACCTTG CTTGGTAAGC GCCAGGAGCT GCTGGTCAGG 
GGCGCCCTGC GCGGCGTTGG GGGCGCTGGG GCCGCTGTGC CGCGCGCCGG GGAGCTGCCG 
GGGGAGGCGG CACAGCGCAG CCGCCTGGAG GAGAAGTTCT TGGAGGAGAA CATCTTGCTG 
CTGCGGAAGC AATTGAATTG TTTGAGGAGA AGAGATGTTG GTTTGTTAAA CCAGTTGCAA 
GAACTTGACA AGCAGATAAG TGACCTAAGA CTTGATGTAG AAAAGACATC TGAAGAGCAC 
TTGGAGACAG ACAGCCGGCC CAGCTCAGGG TTTTATGAGC TGAGTGATGG GGCTTCAGGA 
TCTCTTTCCA ATTCTTCTAA CTCGGTCTTC AGTGAATGTT TGTCCAGTTG TCATTCCAGC 
ACCTGCTTTT GCAGCCCCTT GGAGGCAACC TTGACTATCT CAGATGGTTG CCCCAAATCT 
GCAGATCTCA TAGGATGGTT GGACTATAAA GAAGGCCACT GTGAAGACCA GGCCTCAGAG 
ACAGTTTGCT GTTCCCCCTC CACATCACAA TTTAATCCCC TTGATGTCAT TGCAGATGTG 
AATCCCAAGT ATCAGTGCGA TCTGGTGTCT AAAAACGGGA ATGATGTCTA TCGCTATCCC 
AGTCCGCTTC ATGCCGTGGC TGTGCAGAGC CCAATGTTTC TCCTCTGCCT GACGGGCAAC 
TCCCTGAGGG AAGAGGAGAG GTTGAGTAAC CATGCCAGTG ACATTTGTGT CCGATCTGAG 
CTGGACGGCA TCAAGACAGA CACCTCCTTA CCATCTCCAA GCAGTTTGTG GTCTGCTCCC 
CATCCTTCAT CCAGTAAGAA AATGGATGGC TACATTCTGA GCCTGGTCCA GAAAAAAACA 
CACCCTGTAA GGACCAACAA ACCAAGAACC AGCGTGAACG CTGACCCCAC AAAGGGGCTT 
CTGAGGAATG GGAGTGTTTG TGTCAGAGCG ACTGGAGGCA TCACACAAGG CAATAGTGGG 
AACCTCAAGA ATTCTAAACA GGCGTCTTTG CCCTCTGGTG GAATCCCCTC TCTGGACAAT 
GGGACATTCT CCCCACTGAA GCAGTGGTCG AAGGAATCAA AGGCAGAACA ACTGGAAAGC 
AAGAGGTTGC CCCTGCCTGA GGGCTGCCCC CCTGGAGCTG CCGCTGAACT TCAAAGCAAG 
CATCCGCCCA AAAATGGCAA GCCAGCCTCC CAGGAACATG CTCGGTGTCC CACCACTGTG 
ACAGGGGAGT CCCCTAAAGA AAGCAGTCAG ATCCCAGCTT CCTCACCAAA AGAGAGCCCT 
GGTAGAGCCC TCGTCCCACT GCAGGAAAAC AAAGTTGTCC AGCCACCACT GAAAAAGATG 
GCGCAGAAAA ACAGCCTGCA GGCCGTCCCT CCAGCGGTGC CCCCTCCTGC CCCTTCTGTA 
CTGCTGTCCT CAGCATTTCC GGTGGAAGAA AGGCCTGCCC TGGATTTCAA AAGCGAGGGC 
TCTTCTTCAC AAAGCCTGGA GGATGGGCCT CTGGTGAAGG CTCACTTCGT CCCAGGCCAG 
CAGCCTGGCG TCAGGCCGCA CCGGGGTACC CGGACCATGG CTGTCCCCAG GGGCTCCGCC 
CTGAAGCACA GGGTCCAGGC CCTCCACGGC CCGGACGGCG CCTTGCCCAC CGTGAGGGAG 
AAAACCCGGG CGCCCAGCAA GAAGTGTCGG TTCCCCGACG ACGTGGATAC AAATAAGAAA 
CTCAGGAAGG CCTCAGCCAA GGGGAGGAAG GGCGGGGGCG GCCACGCCGA GGCGGGTTTC 
CCCAGCCGGC CGCTGGGCGC TGCCCACCGG GCGGGGAGCA GGGTGCACGG CCACGGCCGG 
GAGGCCGTGG TGGCCAAGCC CAAGCACAAG CGAACTGACT CGCGGCGCTG GAGGTCGGCC 
GCCGAGCTGT CCTACGAAGA AGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGCGCG 
GGCCTGTACC CGGCCGCGGT GGCGCTGCCC TACGCCAGCC CCTACGCCTA CGTGGCCAGC 
GACTCCGAGT ACTCGGCCGA GTGCGAGTCC CTGTTCCACT CCACCGTAGT GGACACCAGC 
GAGGACGAGC AGAGCAACTA CACTACCAAC TGTTTCGGGG ACAGCGAGTC CAGTGTGAGC 
GAGGGCGAGT TTGGCGGGGA GAGCACGAGC ACCAGCGACT CGGAGGAAAG CGGGGGCCTA 
ATCTGGTCCC AGTTTGTCCA GACTCTCCCG ATTCAGACGG TCACAGCCCC AGACCTTCAC 
AACAACCCCA CGAAAACCTT TGTCAAAATT AAGGCTTCTC ATAACCTCAA GAAGAAAATC 
CTACGCTTTC GGTCTGGCTC TTTGAAGCTG ATGACGACTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DACT1 ( XM_015595820.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_015451306.1 Pteropus alecto dishevelled binding antagonist of beta catenin 1 (DACT1), mRNA.

Price & Availability↑

CloneID	OPt24526
Clone ID Related Accession (Same CDS sequence)	XM_015595820.1 , XM_015595820.2
Accession Version	XM_015595820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	dapper homolog 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006442109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015595820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 6% of CDS bases ##RefSeq-Attributes-END##

ATGTTAAAGA CAGTACCTCA GTCATTTCTC TTCGCTGGTG CAGAAATGAT CCAGAAACCA 
TACTGGAAAT TGCTCTGCTT GCCTTTACGA AGTGGAGTCA AAAAATCCAA CCTCACAGAA 
GTTGTCTTTG AATTCACTCG CGGTACCTTG CTTGGTAAGC GCCAGGAGCT GCTGGTCAGG 
GGCGCCCTGC GCGGCGTTGG GGGCGCTGGG GCCGCTGTGC CGCGCGCCGG GGAGCTGCCG 
GGGGAGGCGG CACAGCGCAG CCGCCTGGAG GAGAAGTTCT TGGAGGAGAA CATCTTGCTG 
CTGCGGAAGC AATTGAATTG TTTGAGGAGA AGAGATGTTG GTTTGTTAAA CCAGTTGCAA 
GAACTTGACA AGCAGATAAG TGACCTAAGA CTTGATGTAG AAAAGACATC TGAAGAGCAC 
TTGGAGACAG ACAGCCGGCC CAGCTCAGGG TTTTATGAGC TGAGTGATGG GGCTTCAGGA 
TCTCTTTCCA ATTCTTCTAA CTCGGTCTTC AGTGAATGTT TGTCCAGTTG TCATTCCAGC 
ACCTGCTTTT GCAGCCCCTT GGAGGCAACC TTGACTATCT CAGATGGTTG CCCCAAATCT 
GCAGATCTCA TAGGATGGTT GGACTATAAA GAAGGCCACT GTGAAGACCA GGCCTCAGAG 
ACAGTTTGCT GTTCCCCCTC CACATCACAA TTTAATCCCC TTGATGTCAT TGCAGATGTG 
AATCCCAAGT ATCAGTGCGA TCTGGTGTCT AAAAACGGGA ATGATGTCTA TCGCTATCCC 
AGTCCGCTTC ATGCCGTGGC TGTGCAGAGC CCAATGTTTC TCCTCTGCCT GACGGGCAAC 
TCCCTGAGGG AAGAGGAGAG GTTGAGTAAC CATGCCAGTG ACATTTGTGT CCGATCTGAG 
CTGGACGGCA TCAAGACAGA CACCTCCTTA CCATCTCCAA GCAGTTTGTG GTCTGCTCCC 
CATCCTTCAT CCAGTAAGAA AATGGATGGC TACATTCTGA GCCTGGTCCA GAAAAAAACA 
CACCCTGTAA GGACCAACAA ACCAAGAACC AGCGTGAACG CTGACCCCAC AAAGGGGCTT 
CTGAGGAATG GGAGTGTTTG TGTCAGAGCG ACTGGAGGCA TCACACAAGG CAATAGTGGG 
AACCTCAAGA ATTCTAAACA GGCGTCTTTG CCCTCTGGTG GAATCCCCTC TCTGGACAAT 
GGGACATTCT CCCCACTGAA GCAGTGGTCG AAGGAATCAA AGGCAGAACA ACTGGAAAGC 
AAGAGGTTGC CCCTGCCTGA GGGCTGCCCC CCTGGAGCTG CCGCTGAACT TCAAAGCAAG 
CATCCGCCCA AAAATGGCAA GCCAGCCTCC CAGGAACATG CTCGGTGTCC CACCACTGTG 
ACAGGGGAGT CCCCTAAAGA AAGCAGTCAG ATCCCAGCTT CCTCACCAAA AGAGAGCCCT 
GGTAGAGCCC TCGTCCCACT GCAGGAAAAC AAAGTTGTCC AGCCACCACT GAAAAAGATG 
GCGCAGAAAA ACAGCCTGCA GGCCGTCCCT CCAGCGGTGC CCCCTCCTGC CCCTTCTGTA 
CTGCTGTCCT CAGCATTTCC GGTGGAAGAA AGGCCTGCCC TGGATTTCAA AAGCGAGGGC 
TCTTCTTCAC AAAGCCTGGA GGATGGGCCT CTGGTGAAGG CTCACTTCGT CCCAGGCCAG 
CAGCCTGGCG TCAGGCCGCA CCGGGGTACC CGGACCATGG CTGTCCCCAG GGGCTCCGCC 
CTGAAGCACA GGGTCCAGGC CCTCCACGGC CCGGACGGCG CCTTGCCCAC CGTGAGGGAG 
AAAACCCGGG CGCCCAGCAA GAAGTGTCGG TTCCCCGACG ACGTGGATAC AAATAAGAAA 
CTCAGGAAGG CCTCAGCCAA GGGGAGGAAG GGCGGGGGCG GCCACGCCGA GGCGGGTTTC 
CCCAGCCGGC CGCTGGGCGC TGCCCACCGG GCGGGGAGCA GGGTGCACGG CCACGGCCGG 
GAGGCCGTGG TGGCCAAGCC CAAGCACAAG CGAACTGACT CGCGGCGCTG GAGGTCGGCC 
GCCGAGCTGT CCTACGAAGA AGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGCGCG 
GGCCTGTACC CGGCCGCGGT GGCGCTGCCC TACGCCAGCC CCTACGCCTA CGTGGCCAGC 
GACTCCGAGT ACTCGGCCGA GTGCGAGTCC CTGTTCCACT CCACCGTAGT GGACACCAGC 
GAGGACGAGC AGAGCAACTA CACTACCAAC TGTTTCGGGG ACAGCGAGTC CAGTGTGAGC 
GAGGGCGAGT TTGGCGGGGA GAGCACGAGC ACCAGCGACT CGGAGGAAAG CGGGGGCCTA 
ATCTGGTCCC AGTTTGTCCA GACTCTCCCG ATTCAGACGG TCACAGCCCC AGACCTTCAC 
AACAACCCCA CGAAAACCTT TGTCAAAATT AAGGCTTCTC ATAACCTCAA GAAGAAAATC 
CTACGCTTTC GGTCTGGCTC TTTGAAGCTG ATGACGACTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015451306.1
CDS	1..2505
Translation	MLKTVPQSFLFAGAEMIQKPYWKLLCLPLRSGVKKSNLTEVVFEFTRGTLLGKRQELLVRGALRGVGGAGAAVPRAGELPGEAAQRSRLEEKFLEENILLLRKQLNCLRRRDVGLLNQLQELDKQISDLRLDVEKTSEEHLETDSRPSSGFYELSDGASGSLSNSSNSVFSECLSSCHSSTCFCSPLEATLTISDGCPKSADLIGWLDYKEGHCEDQASETVCCSPSTSQFNPLDVIADVNPKYQCDLVSKNGNDVYRYPSPLHAVAVQSPMFLLCLTGNSLREEERLSNHASDICVRSELDGIKTDTSLPSPSSLWSAPHPSSSKKMDGYILSLVQKKTHPVRTNKPRTSVNADPTKGLLRNGSVCVRATGGITQGNSGNLKNSKQASLPSGGIPSLDNGTFSPLKQWSKESKAEQLESKRLPLPEGCPPGAAAELQSKHPPKNGKPASQEHARCPTTVTGESPKESSQIPASSPKESPGRALVPLQENKVVQPPLKKMAQKNSLQAVPPAVPPPAPSVLLSSAFPVEERPALDFKSEGSSSQSLEDGPLVKAHFVPGQQPGVRPHRGTRTMAVPRGSALKHRVQALHGPDGALPTVREKTRAPSKKCRFPDDVDTNKKLRKASAKGRKGGGGHAEAGFPSRPLGAAHRAGSRVHGHGREAVVAKPKHKRTDSRRWRSAAELSYEEnullnullnullnullnullnullnullnullnullnullnullnullAGLYPAAVALPYASPYAYVASDSEYSAECESLFHSTVVDTSEDEQSNYTTNCFGDSESSVSEGEFGGESTSTSDSEESGGLIWSQFVQTLPIQTVTAPDLHNNPTKTFVKIKASHNLKKKILRFRSGSLKLMTTV

Target ORF information:

RefSeq Version	XM_015595820.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto dishevelled binding antagonist of beta catenin 1 (DACT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015595820.2

ORF Insert Sequence:

ATGTTAAAGA CAGTACCTCA GTCATTTCTC TTCGCTGGTG CAGAAATGAT CCAGAAACCA 
TACTGGAAAT TGCTCTGCTT GCCTTTACGA AGTGGAGTCA AAAAATCCAA CCTCACAGAA 
GTTGTCTTTG AATTCACTCG CGGTACCTTG CTTGGTAAGC GCCAGGAGCT GCTGGTCAGG 
GGCGCCCTGC GCGGCGTTGG GGGCGCTGGG GCCGCTGTGC CGCGCGCCGG GGAGCTGCCG 
GGGGAGGCGG CACAGCGCAG CCGCCTGGAG GAGAAGTTCT TGGAGGAGAA CATCTTGCTG 
CTGCGGAAGC AATTGAATTG TTTGAGGAGA AGAGATGTTG GTTTGTTAAA CCAGTTGCAA 
GAACTTGACA AGCAGATAAG TGACCTAAGA CTTGATGTAG AAAAGACATC TGAAGAGCAC 
TTGGAGACAG ACAGCCGGCC CAGCTCAGGG TTTTATGAGC TGAGTGATGG GGCTTCAGGA 
TCTCTTTCCA ATTCTTCTAA CTCGGTCTTC AGTGAATGTT TGTCCAGTTG TCATTCCAGC 
ACCTGCTTTT GCAGCCCCTT GGAGGCAACC TTGACTATCT CAGATGGTTG CCCCAAATCT 
GCAGATCTCA TAGGATGGTT GGACTATAAA GAAGGCCACT GTGAAGACCA GGCCTCAGAG 
ACAGTTTGCT GTTCCCCCTC CACATCACAA TTTAATCCCC TTGATGTCAT TGCAGATGTG 
AATCCCAAGT ATCAGTGCGA TCTGGTGTCT AAAAACGGGA ATGATGTCTA TCGCTATCCC 
AGTCCGCTTC ATGCCGTGGC TGTGCAGAGC CCAATGTTTC TCCTCTGCCT GACGGGCAAC 
TCCCTGAGGG AAGAGGAGAG GTTGAGTAAC CATGCCAGTG ACATTTGTGT CCGATCTGAG 
CTGGACGGCA TCAAGACAGA CACCTCCTTA CCATCTCCAA GCAGTTTGTG GTCTGCTCCC 
CATCCTTCAT CCAGTAAGAA AATGGATGGC TACATTCTGA GCCTGGTCCA GAAAAAAACA 
CACCCTGTAA GGACCAACAA ACCAAGAACC AGCGTGAACG CTGACCCCAC AAAGGGGCTT 
CTGAGGAATG GGAGTGTTTG TGTCAGAGCG ACTGGAGGCA TCACACAAGG CAATAGTGGG 
AACCTCAAGA ATTCTAAACA GGCGTCTTTG CCCTCTGGTG GAATCCCCTC TCTGGACAAT 
GGGACATTCT CCCCACTGAA GCAGTGGTCG AAGGAATCAA AGGCAGAACA ACTGGAAAGC 
AAGAGGTTGC CCCTGCCTGA GGGCTGCCCC CCTGGAGCTG CCGCTGAACT TCAAAGCAAG 
CATCCGCCCA AAAATGGCAA GCCAGCCTCC CAGGAACATG CTCGGTGTCC CACCACTGTG 
ACAGGGGAGT CCCCTAAAGA AAGCAGTCAG ATCCCAGCTT CCTCACCAAA AGAGAGCCCT 
GGTAGAGCCC TCGTCCCACT GCAGGAAAAC AAAGTTGTCC AGCCACCACT GAAAAAGATG 
GCGCAGAAAA ACAGCCTGCA GGCCGTCCCT CCAGCGGTGC CCCCTCCTGC CCCTTCTGTA 
CTGCTGTCCT CAGCATTTCC GGTGGAAGAA AGGCCTGCCC TGGATTTCAA AAGCGAGGGC 
TCTTCTTCAC AAAGCCTGGA GGATGGGCCT CTGGTGAAGG CTCACTTCGT CCCAGGCCAG 
CAGCCTGGCG TCAGGCCGCA CCGGGGTACC CGGACCATGG CTGTCCCCAG GGGCTCCGCC 
CTGAAGCACA GGGTCCAGGC CCTCCACGGC CCGGACGGCG CCTTGCCCAC CGTGAGGGAG 
AAAACCCGGG CGCCCAGCAA GAAGTGTCGG TTCCCCGACG ACGTGGATAC AAATAAGAAA 
CTCAGGAAGG CCTCAGCCAA GGGGAGGAAG GGCGGGGGCG GCCACGCCGA GGCGGGTTTC 
CCCAGCCGGC CGCTGGGCGC TGCCCACCGG GCGGGGAGCA GGGTGCACGG CCACGGCCGG 
GAGGCCGTGG TGGCCAAGCC CAAGCACAAG CGAACTGACT CGCGGCGCTG GAGGTCGGCC 
GCCGAGCTGT CCTACGAAGA AGCNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNGCGCG 
GGCCTGTACC CGGCCGCGGT GGCGCTGCCC TACGCCAGCC CCTACGCCTA CGTGGCCAGC 
GACTCCGAGT ACTCGGCCGA GTGCGAGTCC CTGTTCCACT CCACCGTAGT GGACACCAGC 
GAGGACGAGC AGAGCAACTA CACTACCAAC TGTTTCGGGG ACAGCGAGTC CAGTGTGAGC 
GAGGGCGAGT TTGGCGGGGA GAGCACGAGC ACCAGCGACT CGGAGGAAAG CGGGGGCCTA 
ATCTGGTCCC AGTTTGTCCA GACTCTCCCG ATTCAGACGG TCACAGCCCC AGACCTTCAC 
AACAACCCCA CGAAAACCTT TGTCAAAATT AAGGCTTCTC ATAACCTCAA GAAGAAAATC 
CTACGCTTTC GGTCTGGCTC TTTGAAGCTG ATGACGACTG TTTGA