LOC18099600 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC18099600
Entrez Gene ID	18099600
Full Name	hypothetical protein
Gene Type	protein-coding
Organism	Populus trichocarpa(black cottonwood)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006383530.1	XP_006383592.1	hypothetical protein
XM_006383530.2	XP_006383592.2	histone-lysine N-methyltransferase ASHH2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Tuskan GA, Difazio S, Jansson S, Bohlmann J, Grigoriev I, Hellsten U, Putnam N, Ralph S, Rombauts S, Salamov A, Schein J, Sterck L, Aerts A, Bhalerao RR, Bhalerao RP, Blaudez D, Boerjan W, Brun A, Brunner A, Busov V, Campbell M, Carlson J, Chalot M, Chapman J, Chen GL, Cooper D, Coutinho PM, Couturier J, Covert S, Cronk Q, Cunningham R, Davis J, Degroeve S, D?jardin A, Depamphilis C, Detter J, Dirks B, Dubchak I, Duplessis S, Ehlting J, Ellis B, Gendler K, Goodstein D, Gribskov M, Grimwood J, Groover A, Gunter L, Hamberger B, Heinze B, Helariutta Y, Henrissat B, Holligan D, Holt R, Huang W, Islam-Faridi N, Jones S, Jones-Rhoades M, Jorgensen R, Joshi C, Kangasj?rvi J, Karlsson J, Kelleher C, Kirkpatrick R, Kirst M, Kohler A, Kalluri U, Larimer F, Leebens-Mack J, Lepl? JC, Locascio P, Lou Y, Lucas S, Martin F, Montanini B, Napoli C, Nelson DR, Nelson C, Nieminen K, Nilsson O, Pereda V, Peter G, Philippe R, Pilate G, Poliakov A, Razumovskaya J, Richardson P, Rinaldi C, Ritland K, Rouz? P, Ryaboy D, Schmutz J, Schrader J, Segerman B, Shin H, Siddiqui A, Sterky F, Terry A, Tsai CJ, Uberbacher E, Unneberg P, Vahala J, Wall K, Wessler S, Yang G, Yin T, Douglas C, Marra M, Sandberg G, Van de Peer Y, Rokhsar D
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC18099600 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC18099600 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPa14040	XM_006383530.1 Latest version!	Populus trichocarpa hypothetical protein (POPTR_0005s20460g) mRNA, complete cds.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OPa430214	XM_006383530.2 Latest version!	Populus trichocarpa histone-lysine N-methyltransferase ASHH2 (LOC18099600), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC18099600 ( XM_006383530.1 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006383592.1 Populus trichocarpa hypothetical protein (POPTR_0005s20460g) mRNA, complete cds.

Price & Availability↑

CloneID	OPa14040
Clone ID Related Accession (Same CDS sequence)	XM_006383530.1
Accession Version	XM_006383530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-12-30
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Product	hypothetical protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_008471).

ATGGGCGAAG AGGTGACATT TGACTATAAC TATGTAAGAG TTGTTGGGGC TGCTGCTAAA 
AGATGCTATT GTGGTTCGCC TCAATGTCGA GGCTATATTG GTGGTGATCC AACTAGCACT 
GAAGTAGTTG ATCAAGTTGA TTCGGATGAA GAATTTCCTG AACCTGTAAT GCTTGAAGAT 
GGAAGAGTGG GAGGTGGCTT AAAAAATAAA ATATCCAAAA CCAATTTCTT TGGTTTGTCA 
AAAGACAGGG AAATAGAATT CAAAACAGCT GTTGGGAACT TGGAGGTTGC AACTGAAATT 
AAGGACTTGA CAAGCCAATT AACCCCTGCC ATGTCTCTAT CACCCAGTGC ATCAGAAATG 
AATGGTTTAC CTGGAGATTT TTCTTCTTCC AGTCAACAAG TAGAAACCTC CCCAAAGGCT 
GAAGATGTAA TGAGCCAACC CACACCTGCT GTTCAGCAAG AGATTTCCAT GGAAGAGACT 
ATGAACAAAT CCTTGTATTC CAGTGAAAAG TTGAGGACCT CTCCAACTTC AACTCCCACC 
AAAATATTGC CTGATGATGT TATGATTAAC AGGAAGTCTA AGTCTGCCGC AGCTGAAAAC 
AAGAGGGTTT TTGTGAAATC TCGTTTTATT ATTAAAACTC CACACCAATC TAGTTTAATC 
AAGAAAGGAA AGTCTGCTGG TAATCTCATA AACATAAACA AGGTTCAGAC AATAGCCAGC 
AAACCTCAGT TTCCACTTAT CAAACCCAAA AAATTAATAG AGAGTACCTC GAATGGCCAT 
TTTGAAGCAG TTCAGGAGAA ACTTAATGAG TTGTTGGATT CCGAGGGTGG CATAAGCAAA 
CGGAAAGATG CTCCTAAAGG CTACCTGAAA CTTCTCCTCC TTACTGCAGC TTCTGGTGCT 
ATTAGAAGTG GTGAAGCAAT TCAGAGTAAT CGGGAGCTTT CAATGATACT CGATGCACTC 
TTGAAAACTA GATCACGAGT GGTACTGATG GATATAATTA ACAAAAATGG TTTAAGAATG 
CTTCACAACA TAATGAAGCA GTACAGAAGG GACTTTAAAA AGATTCCAAT TCTCAGGAAG 
CTTTTAAAGG TTTTAGAGCA TTTGGCAGTC AGGGAGATAC TTACATTGGA ACATATAAGT 
GGAGGTCCTC CTTGTCCTGG AATGGAGAGC TTTACAGAAT CCATGTTGTC CCTGACAGAG 
CATGATGACA AACAGGTTCA TCAAATTGCA CGAAGTTTTC GAGATAGATG GATCCCTAGA 
CATATCAGAA AGCATAGCTA TATGGATAGG GATGATGGGA GGATGGAGAT TCACAGAGGT 
TCAAACTGCA ATAGGGTTTC AGCTTCTCAT AATCATTGGC ATGATCAGGG TGTAAGACAC 
ACAGAAGCAC TTAATGGTGT TGTGGAATCA AACCTTGCAA TGACTTCAGG GGGCACTGCT 
GTCCATGAGG ACAACTCTGT GAATCGTGTA GGCAGCGGTG CAAGAACTCG CAAGCGTAAA 
ACTAGATGGG ATCAACCCGC TGTGGGAAAT ATAGCTTCCA GCTCTCTTCA GCATATTAAA 
CAGAATGTTA ATTCTGGGTT GGTACAACAA TATGAATCTA ACCCATTACT TGAGTTAAGT 
AAAGAAGTGC CAGTTCATGT GGACAAGGCA GGTAGAGAGT ACAGTTATTG CCCTCATTGT 
GTTCGCAATT ATCGCTGGCA AGATGAATCT AGTAGTGCTG ATGACAGAAA GCAGAATATT 
CATGAGGATG TTCCACCTGG ATTCTCATCT CCGATTAATG CTGCTCTTGC ATCCAATGCT 
TCTTCCACTG TTGCTGACCC CCCTCAACAA AATGTTTTCC ATTTGAAGTT TCCTGTTGGT 
ATGGTTGTCG GTCATCCACA GAAGAAATTC AATTCCCGCT TTCCTGTCTC GTATGGAATT 
CCGTTGTCTG TTATGCAGCA ATTAGGATCA CCCCTGGCTG AAACTGTAGA GAGTTGGGTT 
ATTGCTCCTG GCATGCCTTT CCATCCTTTT CCACCATTAC CACCACTTCC CTCCTGTAAG 
AAAGGGACTC AACCTTCTTG TGCTGTGAGC TCTATGGAGG TTGATGGTGA AGCAGATCGA 
GGGCAACAGG ACAGCCATGA TCCTACCACT TGTCCAAATG AAAGTAGTCC AAGCATGAGT 
GGTGCTAACC AACCAGATGT GAATAGCCCT TGTCCAAATG ACCATCAAAC ATTTAAACGT 
GCGAGGGGAT TTTCATATGA TTTGGGAAGG AGGTACTTTA AGCAACAGAA GTGGAATAAA 
GTGTCTCCTC CATGGGTTCG GAATAGGAAT GGGTGGGGAT GTGTAGGAGA CAACTCAAGA 
GGTGGAATGT GCAGCACTGA CATGGGAAGT CTAACAAATG AACAGAGGAA CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006383592.1
CDS	56..2452
Translation	MGEEVTFDYNYVRVVGAAAKRCYCGSPQCRGYIGGDPTSTEVVDQVDSDEEFPEPVMLEDGRVGGGLKNKISKTNFFGLSKDREIEFKTAVGNLEVATEIKDLTSQLTPAMSLSPSASEMNGLPGDFSSSSQQVETSPKAEDVMSQPTPAVQQEISMEETMNKSLYSSEKLRTSPTSTPTKILPDDVMINRKSKSAAAENKRVFVKSRFIIKTPHQSSLIKKGKSAGNLININKVQTIASKPQFPLIKPKKLIESTSNGHFEAVQEKLNELLDSEGGISKRKDAPKGYLKLLLLTAASGAIRSGEAIQSNRELSMILDALLKTRSRVVLMDIINKNGLRMLHNIMKQYRRDFKKIPILRKLLKVLEHLAVREILTLEHISGGPPCPGMESFTESMLSLTEHDDKQVHQIARSFRDRWIPRHIRKHSYMDRDDGRMEIHRGSNCNRVSASHNHWHDQGVRHTEALNGVVESNLAMTSGGTAVHEDNSVNRVGSGARTRKRKTRWDQPAVGNIASSSLQHIKQNVNSGLVQQYESNPLLELSKEVPVHVDKAGREYSYCPHCVRNYRWQDESSSADDRKQNIHEDVPPGFSSPINAALASNASSTVADPPQQNVFHLKFPVGMVVGHPQKKFNSRFPVSYGIPLSVMQQLGSPLAETVESWVIAPGMPFHPFPPLPPLPSCKKGTQPSCAVSSMEVDGEADRGQQDSHDPTTCPNESSPSMSGANQPDVNSPCPNDHQTFKRARGFSYDLGRRYFKQQKWNKVSPPWVRNRNGWGCVGDNSRGGMCSTDMGSLTNEQRNS

Target ORF information:

RefSeq Version	XM_006383530.1
Organism	Populus trichocarpa(Populus balsamifera subsp. trichocarpa)
Definition	Populus trichocarpa hypothetical protein (POPTR_0005s20460g) mRNA, complete cds.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006383530.1

ORF Insert Sequence:

ATGGGCGAAG AGGTGACATT TGACTATAAC TATGTAAGAG TTGTTGGGGC TGCTGCTAAA 
AGATGCTATT GTGGTTCGCC TCAATGTCGA GGCTATATTG GTGGTGATCC AACTAGCACT 
GAAGTAGTTG ATCAAGTTGA TTCGGATGAA GAATTTCCTG AACCTGTAAT GCTTGAAGAT 
GGAAGAGTGG GAGGTGGCTT AAAAAATAAA ATATCCAAAA CCAATTTCTT TGGTTTGTCA 
AAAGACAGGG AAATAGAATT CAAAACAGCT GTTGGGAACT TGGAGGTTGC AACTGAAATT 
AAGGACTTGA CAAGCCAATT AACCCCTGCC ATGTCTCTAT CACCCAGTGC ATCAGAAATG 
AATGGTTTAC CTGGAGATTT TTCTTCTTCC AGTCAACAAG TAGAAACCTC CCCAAAGGCT 
GAAGATGTAA TGAGCCAACC CACACCTGCT GTTCAGCAAG AGATTTCCAT GGAAGAGACT 
ATGAACAAAT CCTTGTATTC CAGTGAAAAG TTGAGGACCT CTCCAACTTC AACTCCCACC 
AAAATATTGC CTGATGATGT TATGATTAAC AGGAAGTCTA AGTCTGCCGC AGCTGAAAAC 
AAGAGGGTTT TTGTGAAATC TCGTTTTATT ATTAAAACTC CACACCAATC TAGTTTAATC 
AAGAAAGGAA AGTCTGCTGG TAATCTCATA AACATAAACA AGGTTCAGAC AATAGCCAGC 
AAACCTCAGT TTCCACTTAT CAAACCCAAA AAATTAATAG AGAGTACCTC GAATGGCCAT 
TTTGAAGCAG TTCAGGAGAA ACTTAATGAG TTGTTGGATT CCGAGGGTGG CATAAGCAAA 
CGGAAAGATG CTCCTAAAGG CTACCTGAAA CTTCTCCTCC TTACTGCAGC TTCTGGTGCT 
ATTAGAAGTG GTGAAGCAAT TCAGAGTAAT CGGGAGCTTT CAATGATACT CGATGCACTC 
TTGAAAACTA GATCACGAGT GGTACTGATG GATATAATTA ACAAAAATGG TTTAAGAATG 
CTTCACAACA TAATGAAGCA GTACAGAAGG GACTTTAAAA AGATTCCAAT TCTCAGGAAG 
CTTTTAAAGG TTTTAGAGCA TTTGGCAGTC AGGGAGATAC TTACATTGGA ACATATAAGT 
GGAGGTCCTC CTTGTCCTGG AATGGAGAGC TTTACAGAAT CCATGTTGTC CCTGACAGAG 
CATGATGACA AACAGGTTCA TCAAATTGCA CGAAGTTTTC GAGATAGATG GATCCCTAGA 
CATATCAGAA AGCATAGCTA TATGGATAGG GATGATGGGA GGATGGAGAT TCACAGAGGT 
TCAAACTGCA ATAGGGTTTC AGCTTCTCAT AATCATTGGC ATGATCAGGG TGTAAGACAC 
ACAGAAGCAC TTAATGGTGT TGTGGAATCA AACCTTGCAA TGACTTCAGG GGGCACTGCT 
GTCCATGAGG ACAACTCTGT GAATCGTGTA GGCAGCGGTG CAAGAACTCG CAAGCGTAAA 
ACTAGATGGG ATCAACCCGC TGTGGGAAAT ATAGCTTCCA GCTCTCTTCA GCATATTAAA 
CAGAATGTTA ATTCTGGGTT GGTACAACAA TATGAATCTA ACCCATTACT TGAGTTAAGT 
AAAGAAGTGC CAGTTCATGT GGACAAGGCA GGTAGAGAGT ACAGTTATTG CCCTCATTGT 
GTTCGCAATT ATCGCTGGCA AGATGAATCT AGTAGTGCTG ATGACAGAAA GCAGAATATT 
CATGAGGATG TTCCACCTGG ATTCTCATCT CCGATTAATG CTGCTCTTGC ATCCAATGCT 
TCTTCCACTG TTGCTGACCC CCCTCAACAA AATGTTTTCC ATTTGAAGTT TCCTGTTGGT 
ATGGTTGTCG GTCATCCACA GAAGAAATTC AATTCCCGCT TTCCTGTCTC GTATGGAATT 
CCGTTGTCTG TTATGCAGCA ATTAGGATCA CCCCTGGCTG AAACTGTAGA GAGTTGGGTT 
ATTGCTCCTG GCATGCCTTT CCATCCTTTT CCACCATTAC CACCACTTCC CTCCTGTAAG 
AAAGGGACTC AACCTTCTTG TGCTGTGAGC TCTATGGAGG TTGATGGTGA AGCAGATCGA 
GGGCAACAGG ACAGCCATGA TCCTACCACT TGTCCAAATG AAAGTAGTCC AAGCATGAGT 
GGTGCTAACC AACCAGATGT GAATAGCCCT TGTCCAAATG ACCATCAAAC ATTTAAACGT 
GCGAGGGGAT TTTCATATGA TTTGGGAAGG AGGTACTTTA AGCAACAGAA GTGGAATAAA 
GTGTCTCCTC CATGGGTTCG GAATAGGAAT GGGTGGGGAT GTGTAGGAGA CAACTCAAGA 
GGTGGAATGT GCAGCACTGA CATGGGAAGT CTAACAAATG AACAGAGGAA CTCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC18099600 ( XM_006383530.2 ) cDNA ORF clone, Populus trichocarpa(black cottonwood) -> XP_006383592.2 Populus trichocarpa histone-lysine N-methyltransferase ASHH2 (LOC18099600), mRNA.

Price & Availability↑

CloneID	OPa430214
Clone ID Related Accession (Same CDS sequence)	XM_006383530.2
Accession Version	XM_006383530.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6834bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-08
Organism	Populus trichocarpa(black cottonwood)
Product	histone-lysine N-methyltransferase ASHH2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037289.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 9, 2018 this sequence version replaced XM_006383530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Populus trichocarpa Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGCGT GTGAGAATTC GTTTGAGCCA CACGAGCCCT TGACTCTTGC TGCCACCGAG 
CAGCATTCGT GCTTGGAATT TATCAAGGAT TCTGAGCAGC TGCCAGTCTT GGAGACTGCT 
CACAGTCTAA TTGATACTAA TGTGGAACCC TCTAGTGCGA CTGATGGGTT TGTGGACTTG 
TCTCAGAAAG ATAATGTTGT TTGTGCCAGC CACAGTGATG TTAAGGCTGT GTCAGCTGAT 
ATGGGAATTG GGTTGACGGG TGAGGGGGAG AGTGTGGTTG GTTTGACAGC AGGGAAATTA 
TTGGAGGATG AATCTGGGGT TATTGGGGGT TGTTTGGATG AGAGGCAAAG TGGGATAGAT 
GATTGTAATG GGGAAACGGA TAGATCCCGG GAAGAAAAAG CAGGGCTTGG AGTCAAGAAT 
GGTGGACCAC TAGACTGTGA AAGGTCTTTG GAATTGCTGG AGAGAAATTG TGATCAACAG 
GATGGCCGAG TGGATGATAA GAAAACTGGT GTTCAAGGTG TTACAGAAGA GGAAAGTGAT 
GGTTTAGTTA CAGTAGAGGC TGATACTTCC GATGAAGTAG TGCCTTCAAC CTCATGTGAA 
ACATCTGTAG AATCGATACT TGGGAATGAT ATGACTAGAA ATTGTGATCA ACAAGATGAC 
CAGAATGATC ATGAGAGTGG CAGTGTTCAA GGGGTTATGG AAGAGAAAAA TGATGGTTTA 
GCTGCAATAA AGATTGTTAA TTCTGATGAA ATAGTTCTTT CACTGGGCAG TGAGATGCCA 
ACAGAATTAA TGCAAGCAAA GGATTGGAGC AGAAATGGTG ATCAACAAGA TGACCAGAGG 
GATGATAAGA ATGTCAGTGT TCAAGGGGTC ATGGAACAAC ATAGTAGTGG CTTAGCTCCA 
ATAGAGTTTG ATGATATACA TCATGAAATA GTGCTTTCAT CGGGTCAAGG AATGCCTGCA 
GAACTACTAC CAGAAAAAGG TTTGCCTAGT GATGGCAATG AACAGTACAA GCATGATTGT 
GGTACCTCTC AAGAAGCAGT TATGGAAGAG AAAATTGACA ATTTAACTGG GCTAGAGAAT 
AGAGACCTTC AAGCTGTGAT GGAACAGAAA AGTGATGGTT TAGTGGCAAC AAAGACTGCT 
GATACTTGTG AAAACATATT GCCTTCATTG GGATATGAAA TGCCCGCTGA GCGTTTGCCT 
AGAAATGGTG TTGAGAAGGA CAAGCAAGAC AGGAGCACCT CTATGGTGCT GACCATGGAA 
GAGAGAAACA ATGATTTAGC TGGGACAGAG AGTATTAGTA TGGAAGGGTT CATGGAAGAA 
AAAAGTGATG GTTTAGCTGC AATAGAGACT GCTACTCATA ATGAAATAGG ACCTTTGTCA 
GGCTGTGAAA TCCCTGCTGG ATTAATTTCA GTGAATGGTT ATGGTGTTGA ACTGGACAAG 
CAGTATGATG GTACGTCTCC TACTGTGGTT CCAAAAGAAA GAAGTGTTCT TTTAACTAGG 
TTAGAGACTG ATAATCAAGA CCAAATATTG CCTTCACTTG ACCATGGAAT GCATTTGGAA 
TCAACAACTG TTACATGTCC ACCAAGCAAG TGTCTTCAGC CGGATGACCA GAAGGGTGAT 
CAGATCATTA GCTGTCTCAT TGCAGGAGGG GTTATGGAGG AAACAAGTGA TGCTTTAGAT 
GCGACAGATA CTACTACTTC CAATTGGTTG TTGTCATCAC AGGGCCTCGA GAGGACCTTG 
AAATTAATGC CCATGACTGG TTTGCCAGAA GAAAGTGTTT ATCATGATGA GCAGAAGCTG 
ATACCAAGTG AACTAGATTC TAAGGTTGTC AATGGTCTAG CTATAGAGAG GGTTCCAGAG 
CAGGAAAGCG ATGCCTCAGC CAGGATAGGA GCTGATATAT ATGCCCAGGT ATCACCACAT 
GGTGCTATTG ACTCTAACAG TGCAGGTGAC TGTTCTGGAG AGACGGTCAA TGAAGCAAAG 
AATCATGTCA GCATTGATAG TGTTTCTGAA ACCAAGTGTC ATGACATTGC ATCACCATCT 
TCACAAAGGA GCAATGGAGT ACGCAAATCA AGCCGGAAGG CCCAGACAAA AAGGGCTGCA 
AGGAAATCTA GAAATACAAC CAAAGTCCCA AACCTTCATC TAGGTATTGA GACTGTCTTC 
AAAAGTGTGA CAAGGAAGCG AAGCTGTTTC TCCAAACCAG CTCGGTCTTC TGCTTGGGGA 
TTGTTGGGGA ATATTACACA TGCATTTACA ATGATTGATG GTCCCAGACT TGATGAAATT 
GAGAATAATG GATCACAAAA AGCAAGGGGT GGCAGAGGAA GCAGAAAGCG GAATAATCGT 
GCTGGTGGAA GGTCACAAAG GTCTAGCAAG AAAGGCTGTG CTTCAGCTAG TTGCATTCGT 
TTGAAAGTTA AAGTGGGAAA AGAAGCTTGT CAAACTGAAG CTAATCCGAA GATTATGATC 
CCAGAGGTAA TTGATACAAA AGCATCTGCT GAACTTGTAA GCAATTATGG GGTTGAGTCA 
TATCAGGAAA CCAGTTTTGA AATGTCAAAG TTAGTCCATT ATGCTGAAGA TAATGTGGCT 
GAAGAGGGAG CTGGAAAACA GCTTCAGTCT TTTGATATTA AGTTGAAGGC AGAGTTACAT 
TGCGATCCTT ATGGCATGGA TGTAAATCTT GCAAACAAAG ATATGGAGGG CATGGTGATT 
TTTGAAAAGT CACCTGGAGA TACTGTGGAA GATTATATCG GGGTTCCTCT CCATACAGAG 
GTTGAAGCAC TGGGAGCAAC AACTGAGAAG AGGTATACAG ATCCTGGAAC TTCACCAGAT 
TCAGAAGTTA TCAATTTAGT TGCCGAGGGC CAAGTCGATG CACGATGTCC AGAAGATTTT 
CATGATGCTG TTCTGTCTTC TTCTAAGGCT TTTGCTACAG ATCAAGGGGG TAATGGTAAA 
AGGAGGGGAA AAAAGAAAGA AAGGCTCCCT CAAGCCGCTA ACTGTTCACC TGCTGCGGCA 
AGCTTAAACA AAGTTAAATT GGCAAAGAAA CGTGGGGGCA GGCAGAGAAA GGCTGATGGC 
CTTTCCTCTA CTGAGATTCT TACTTCATCC AGCAGTGTGA ATGGTTTGAT CAACACACCA 
AGCAGTAAAG AATGTTCCGC AGAACAGGTA CCTTTGTCAA GAGAGACTGA ACTTGAAGTC 
TCTGGAGAGG TTTTGACAGA AGAAATCAGT ATGGAAACTA AAATATGTGT TGGTGGACTG 
GATGCTGAGC TTAGGTCATC AGAATCACAG ATTTCAAAGA ATCCTCTTCC TTCAACTAAA 
TCCAGGGGAA ATGGAGTGAA CAAGGGAAGG TCCAAAGTCT CCGACTCTGC AAAAAGCCGG 
AGGGCAAATG GTTGCAAGGA TAGAGGAAAC GATAGGAAGT CAGTTAAAAA GAACAAAGCC 
AAGGGGAAGA GTGTTTGTGA TCATGTTTTT TACAAAGTTG ATGATGATCC AGAAATAGGT 
CAAGAAGCAT TCTGCTCTGA CATGAAGTAT CGCTGTAATC ATACTGCAGA TGAAAATGGA 
AAAATTGATG CTGTTGAGGA TACTGCTGCA GAAGAGGTAG CTGATTTGGA CACGCCGTCA 
AGTGGTGTAA TGGAGCAAAA TTTATCCCCA GATAATGCTT GGGTTCGCTG TGATGATTGT 
CTTAAATGGC GGCGTATTCC AGTTCGACTT GTAGAATCTA TTAGTCAAAC ACATTGCCAA 
TGGATCTGCA AGGACAACAT GAATAAAGCC TTTGCTGATT GCTCCTTCCC CCAAGAGAAG 
TCAAATGCAG AAATAAATGC AGAGTTGGGC ATATCAGATG TTGATGAGGA TGGTTGCGAT 
GCCCCTTCAA ATTATATGGA ATTGGAATGT AGACAGACAT CAGTTTCCAA GGAGTATGAA 
TTTACACGCA TCACTACCAA TCAGTTTCTG CACCGTAGCC GTAAAACTCA AACTATTGAT 
GAGATCATGG TTTGTTACTG CAAAGCACCT GTGGCCGGTC GGTTAGGTTG TGGAGATGAA 
TGCTTGAATC GAATGCTTAA TATTGAGTGT GTTCAAGGAA CCTGTCCATG TGGGGACCAT 
TGCTCAAATC AACAGTTCCA AAAGCGCAAT TATGCCAAAA TGACATGGGA GCGATGTGGG 
AAGAAAGGTT TTGGACTGCG ATTGGATGAG GATATATCCA GAGGGCAATT CCTTATTGAA 
TACGTTGGAG AGGTGCTTGA TGTGCATGCT TATGAAGCAA GGCAAAAAGA TTATGCTTCC 
AAGGGTCACA AACATTTCTA CTTCATGACA TTGGATGGCA GTGAGGTAAT TGACGCATGT 
GCGAAGGGAA ATTTGGGGCG TTTCATTAAT CATAGTTGTG ATCCTAATTG TCGTACTGAA 
AAGTGGGTGG TCAATGGAGA AATCTGTATT GGATTATTTG CGTTGAGGGA CATTAAAATG 
GGCGAAGAGG TGACATTTGA CTATAACTAT GTAAGAGTTG TTGGGGCTGC TGCTAAAAGA 
TGCTATTGTG GTTCGCCTCA ATGTCGAGGC TATATTGGTG GTGATCCAAC TAGCACTGAA 
GTAGTTGATC AAGTTGATTC GGATGAAGAA TTTCCTGAAC CTGTAATGCT TGAAGATGGA 
AGAGTGGGAG GTGGCTTAAA AAATAAAATA TCCAAAACCA ATTTCTTTGG TTTGTCAAAA 
GACAGGGAAA TAGAATTCAA AACAGCTGTT GGGAACTTGG AGGTTGCAAC TGAAATTAAG 
GACTTGACAA GCCAATTAAC CCCTGCCATG TCTCTATCAC CCAGTGCATC AGAAATGAAT 
GGTTTACCTG GAGATTTTTC TTCTTCCAGT CAACAAGTAG AAACCTCCCC AAAGGCTGAA 
GATGTAATGA GCCAACCCAC ACCTGCTGTT CAGCAAGAGA TTTCCATGGA AGAGACTATG 
AACAAATCCT TGTATTCCAG TGAAAAGTTG AGGACCTCTC CAACTTCAAC TCCCACCAAA 
ATATTGCCTG ATGATGTTAT GATTAACAGG AAGTCTAAGT CTGCCGCAGC TGAAAACAAG 
AGGGTTTTTG TGAAATCTCG TTTTATTATT AAAACTCCAC ACCAATCTAG TTTAATCAAG 
AAAGGAAAGT CTGCTGGTAA TCTCATAAAC ATAAACAAGG TTCAGACAAT AGCCAGCAAA 
CCTCAGTTTC CACTTATCAA ACCCAAAAAA TTAATAGAGA GTACCTCGAA TGGCCATTTT 
GAAGCAGTTC AGGAGAAACT TAATGAGTTG TTGGATTCCG AGGGTGGCAT AAGCAAACGG 
AAAGATGCTC CTAAAGGCTA CCTGAAACTT CTCCTCCTTA CTGCAGCTTC TGGTGCTATT 
AGAAGTGGTG AAGCAATTCA GAGTAATCGG GAGCTTTCAA TGATACTCGA TGCACTCTTG 
AAAACTAGAT CACGAGTGGT ACTGATGGAT ATAATTAACA AAAATGGTTT AAGAATGCTT 
CACAACATAA TGAAGCAGTA CAGAAGGGAC TTTAAAAAGA TTCCAATTCT CAGGAAGCTT 
TTAAAGGTTT TAGAGCATTT GGCAGTCAGG GAGATACTTA CATTGGAACA TATAAGTGGA 
GGTCCTCCTT GTCCTGGAAT GGAGAGCTTT ACAGAATCCA TGTTGTCCCT GACAGAGCAT 
GATGACAAAC AGGTTCATCA AATTGCACGA AGTTTTCGAG ATAGATGGAT CCCTAGACAT 
ATCAGAAAGC ATAGCTATAT GGATAGGGAT GATGGGAGGA TGGAGATTCA CAGAGGTTCA 
AACTGCAATA GGGTTTCAGC TTCTCATAAT CATTGGCATG ATCAGGGTGT AAGACACACA 
GAAGCACTTA ATGGTGTTGT GGAATCAAAC CTTGCAATGA CTTCAGGGGG CACTGCTGTC 
CATGAGGACA ACTCTGTGAA TCGTGTAGGC AGCGGTGCAA GAACTCGCAA GCGTAAAACT 
AGATGGGATC AACCCGCTGT GGGAAATATA GCTTCCAGCT CTCTTCAGCA TATTAAACAG 
AATGTTAATT CTGGGTTGGT ACAACAATAT GAATCTAACC CATTACTTGA GTTAAGTAAA 
GAAGTGCCAG TTCATGTGGA CAAGGCAGGT AGAGAGTACA GTTATTGCCC TCATTGTGTT 
CGCAATTATC GCTGGCAAGA TGAATCTAGT AGTGCTGATG ACAGAAAGCA GAATATTCAT 
GAGGATGTTC CACCTGGATT CTCATCTCCG ATTAATGCTG CTCTTGCATC CAATGCTTCT 
TCCACTGTTG CTGACCCCCC TCAACAAAAT GTTTTCCATT TGAAGTTTCC TGTTGGTATG 
GTTGTCGGTC ATCCACAGAA GAAATTCAAT TCCCGCTTTC CTGTCTCGTA TGGAATTCCG 
TTGTCTGTTA TGCAGCAATT AGGATCACCC CTGGCTGAAA CTGTAGAGAG TTGGGTTATT 
GCTCCTGGCA TGCCTTTCCA TCCTTTTCCA CCATTACCAC CACTTCCCTC CTGTAAGAAA 
GGGACTCAAC CTTCTTGTGC TGTGAGCTCT ATGGAGGTTG ATGGTGAAGC AGATCGAGGG 
CAACAGGACA GCCATGATCC TACCACTTGT CCAAATGAAA GTAGTCCAAG CATGAGTGGT 
GCTAACCAAC CAGATGTGAA TAGCCCTTGT CCAAATGACC ATCAAACATT TAAACGTGCG 
AGGGGATTTT CATATGATTT GGGAAGGAGG TACTTTAAGC AACAGAAGTG GAATAAAGTG 
TCTCCTCCAT GGGTTCGGAA TAGGAATGGG TGGGGATGTG TAGGAGACAA CTCAAGAGGT 
GGAATGTGCA GCACTGACAT GGGAAGTCTA ACAAATGAAC AGAGGAACTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006383592.2
CDS	496..7329
Translation	MGACENSFEPHEPLTLAATEQHSCLEFIKDSEQLPVLETAHSLIDTNVEPSSATDGFVDLSQKDNVVCASHSDVKAVSADMGIGLTGEGESVVGLTAGKLLEDESGVIGGCLDERQSGIDDCNGETDRSREEKAGLGVKNGGPLDCERSLELLERNCDQQDGRVDDKKTGVQGVTEEESDGLVTVEADTSDEVVPSTSCETSVESILGNDMTRNCDQQDDQNDHESGSVQGVMEEKNDGLAAIKIVNSDEIVLSLGSEMPTELMQAKDWSRNGDQQDDQRDDKNVSVQGVMEQHSSGLAPIEFDDIHHEIVLSSGQGMPAELLPEKGLPSDGNEQYKHDCGTSQEAVMEEKIDNLTGLENRDLQAVMEQKSDGLVATKTADTCENILPSLGYEMPAERLPRNGVEKDKQDRSTSMVLTMEERNNDLAGTESISMEGFMEEKSDGLAAIETATHNEIGPLSGCEIPAGLISVNGYGVELDKQYDGTSPTVVPKERSVLLTRLETDNQDQILPSLDHGMHLESTTVTCPPSKCLQPDDQKGDQIISCLIAGGVMEETSDALDATDTTTSNWLLSSQGLERTLKLMPMTGLPEESVYHDEQKLIPSELDSKVVNGLAIERVPEQESDASARIGADIYAQVSPHGAIDSNSAGDCSGETVNEAKNHVSIDSVSETKCHDIASPSSQRSNGVRKSSRKAQTKRAARKSRNTTKVPNLHLGIETVFKSVTRKRSCFSKPARSSAWGLLGNITHAFTMIDGPRLDEIENNGSQKARGGRGSRKRNNRAGGRSQRSSKKGCASASCIRLKVKVGKEACQTEANPKIMIPEVIDTKASAELVSNYGVESYQETSFEMSKLVHYAEDNVAEEGAGKQLQSFDIKLKAELHCDPYGMDVNLANKDMEGMVIFEKSPGDTVEDYIGVPLHTEVEALGATTEKRYTDPGTSPDSEVINLVAEGQVDARCPEDFHDAVLSSSKAFATDQGGNGKRRGKKKERLPQAANCSPAAASLNKVKLAKKRGGRQRKADGLSSTEILTSSSSVNGLINTPSSKECSAEQVPLSRETELEVSGEVLTEEISMETKICVGGLDAELRSSESQISKNPLPSTKSRGNGVNKGRSKVSDSAKSRRANGCKDRGNDRKSVKKNKAKGKSVCDHVFYKVDDDPEIGQEAFCSDMKYRCNHTADENGKIDAVEDTAAEEVADLDTPSSGVMEQNLSPDNAWVRCDDCLKWRRIPVRLVESISQTHCQWICKDNMNKAFADCSFPQEKSNAEINAELGISDVDEDGCDAPSNYMELECRQTSVSKEYEFTRITTNQFLHRSRKTQTIDEIMVCYCKAPVAGRLGCGDECLNRMLNIECVQGTCPCGDHCSNQQFQKRNYAKMTWERCGKKGFGLRLDEDISRGQFLIEYVGEVLDVHAYEARQKDYASKGHKHFYFMTLDGSEVIDACAKGNLGRFINHSCDPNCRTEKWVVNGEICIGLFALRDIKMGEEVTFDYNYVRVVGAAAKRCYCGSPQCRGYIGGDPTSTEVVDQVDSDEEFPEPVMLEDGRVGGGLKNKISKTNFFGLSKDREIEFKTAVGNLEVATEIKDLTSQLTPAMSLSPSASEMNGLPGDFSSSSQQVETSPKAEDVMSQPTPAVQQEISMEETMNKSLYSSEKLRTSPTSTPTKILPDDVMINRKSKSAAAENKRVFVKSRFIIKTPHQSSLIKKGKSAGNLININKVQTIASKPQFPLIKPKKLIESTSNGHFEAVQEKLNELLDSEGGISKRKDAPKGYLKLLLLTAASGAIRSGEAIQSNRELSMILDALLKTRSRVVLMDIINKNGLRMLHNIMKQYRRDFKKIPILRKLLKVLEHLAVREILTLEHISGGPPCPGMESFTESMLSLTEHDDKQVHQIARSFRDRWIPRHIRKHSYMDRDDGRMEIHRGSNCNRVSASHNHWHDQGVRHTEALNGVVESNLAMTSGGTAVHEDNSVNRVGSGARTRKRKTRWDQPAVGNIASSSLQHIKQNVNSGLVQQYESNPLLELSKEVPVHVDKAGREYSYCPHCVRNYRWQDESSSADDRKQNIHEDVPPGFSSPINAALASNASSTVADPPQQNVFHLKFPVGMVVGHPQKKFNSRFPVSYGIPLSVMQQLGSPLAETVESWVIAPGMPFHPFPPLPPLPSCKKGTQPSCAVSSMEVDGEADRGQQDSHDPTTCPNESSPSMSGANQPDVNSPCPNDHQTFKRARGFSYDLGRRYFKQQKWNKVSPPWVRNRNGWGCVGDNSRGGMCSTDMGSLTNEQRNS

Target ORF information:

RefSeq Version	XM_006383530.2
Organism	Populus trichocarpa(black cottonwood)
Definition	Populus trichocarpa histone-lysine N-methyltransferase ASHH2 (LOC18099600), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006383530.2

ORF Insert Sequence:

ATGGGGGCGT GTGAGAATTC GTTTGAGCCA CACGAGCCCT TGACTCTTGC TGCCACCGAG 
CAGCATTCGT GCTTGGAATT TATCAAGGAT TCTGAGCAGC TGCCAGTCTT GGAGACTGCT 
CACAGTCTAA TTGATACTAA TGTGGAACCC TCTAGTGCGA CTGATGGGTT TGTGGACTTG 
TCTCAGAAAG ATAATGTTGT TTGTGCCAGC CACAGTGATG TTAAGGCTGT GTCAGCTGAT 
ATGGGAATTG GGTTGACGGG TGAGGGGGAG AGTGTGGTTG GTTTGACAGC AGGGAAATTA 
TTGGAGGATG AATCTGGGGT TATTGGGGGT TGTTTGGATG AGAGGCAAAG TGGGATAGAT 
GATTGTAATG GGGAAACGGA TAGATCCCGG GAAGAAAAAG CAGGGCTTGG AGTCAAGAAT 
GGTGGACCAC TAGACTGTGA AAGGTCTTTG GAATTGCTGG AGAGAAATTG TGATCAACAG 
GATGGCCGAG TGGATGATAA GAAAACTGGT GTTCAAGGTG TTACAGAAGA GGAAAGTGAT 
GGTTTAGTTA CAGTAGAGGC TGATACTTCC GATGAAGTAG TGCCTTCAAC CTCATGTGAA 
ACATCTGTAG AATCGATACT TGGGAATGAT ATGACTAGAA ATTGTGATCA ACAAGATGAC 
CAGAATGATC ATGAGAGTGG CAGTGTTCAA GGGGTTATGG AAGAGAAAAA TGATGGTTTA 
GCTGCAATAA AGATTGTTAA TTCTGATGAA ATAGTTCTTT CACTGGGCAG TGAGATGCCA 
ACAGAATTAA TGCAAGCAAA GGATTGGAGC AGAAATGGTG ATCAACAAGA TGACCAGAGG 
GATGATAAGA ATGTCAGTGT TCAAGGGGTC ATGGAACAAC ATAGTAGTGG CTTAGCTCCA 
ATAGAGTTTG ATGATATACA TCATGAAATA GTGCTTTCAT CGGGTCAAGG AATGCCTGCA 
GAACTACTAC CAGAAAAAGG TTTGCCTAGT GATGGCAATG AACAGTACAA GCATGATTGT 
GGTACCTCTC AAGAAGCAGT TATGGAAGAG AAAATTGACA ATTTAACTGG GCTAGAGAAT 
AGAGACCTTC AAGCTGTGAT GGAACAGAAA AGTGATGGTT TAGTGGCAAC AAAGACTGCT 
GATACTTGTG AAAACATATT GCCTTCATTG GGATATGAAA TGCCCGCTGA GCGTTTGCCT 
AGAAATGGTG TTGAGAAGGA CAAGCAAGAC AGGAGCACCT CTATGGTGCT GACCATGGAA 
GAGAGAAACA ATGATTTAGC TGGGACAGAG AGTATTAGTA TGGAAGGGTT CATGGAAGAA 
AAAAGTGATG GTTTAGCTGC AATAGAGACT GCTACTCATA ATGAAATAGG ACCTTTGTCA 
GGCTGTGAAA TCCCTGCTGG ATTAATTTCA GTGAATGGTT ATGGTGTTGA ACTGGACAAG 
CAGTATGATG GTACGTCTCC TACTGTGGTT CCAAAAGAAA GAAGTGTTCT TTTAACTAGG 
TTAGAGACTG ATAATCAAGA CCAAATATTG CCTTCACTTG ACCATGGAAT GCATTTGGAA 
TCAACAACTG TTACATGTCC ACCAAGCAAG TGTCTTCAGC CGGATGACCA GAAGGGTGAT 
CAGATCATTA GCTGTCTCAT TGCAGGAGGG GTTATGGAGG AAACAAGTGA TGCTTTAGAT 
GCGACAGATA CTACTACTTC CAATTGGTTG TTGTCATCAC AGGGCCTCGA GAGGACCTTG 
AAATTAATGC CCATGACTGG TTTGCCAGAA GAAAGTGTTT ATCATGATGA GCAGAAGCTG 
ATACCAAGTG AACTAGATTC TAAGGTTGTC AATGGTCTAG CTATAGAGAG GGTTCCAGAG 
CAGGAAAGCG ATGCCTCAGC CAGGATAGGA GCTGATATAT ATGCCCAGGT ATCACCACAT 
GGTGCTATTG ACTCTAACAG TGCAGGTGAC TGTTCTGGAG AGACGGTCAA TGAAGCAAAG 
AATCATGTCA GCATTGATAG TGTTTCTGAA ACCAAGTGTC ATGACATTGC ATCACCATCT 
TCACAAAGGA GCAATGGAGT ACGCAAATCA AGCCGGAAGG CCCAGACAAA AAGGGCTGCA 
AGGAAATCTA GAAATACAAC CAAAGTCCCA AACCTTCATC TAGGTATTGA GACTGTCTTC 
AAAAGTGTGA CAAGGAAGCG AAGCTGTTTC TCCAAACCAG CTCGGTCTTC TGCTTGGGGA 
TTGTTGGGGA ATATTACACA TGCATTTACA ATGATTGATG GTCCCAGACT TGATGAAATT 
GAGAATAATG GATCACAAAA AGCAAGGGGT GGCAGAGGAA GCAGAAAGCG GAATAATCGT 
GCTGGTGGAA GGTCACAAAG GTCTAGCAAG AAAGGCTGTG CTTCAGCTAG TTGCATTCGT 
TTGAAAGTTA AAGTGGGAAA AGAAGCTTGT CAAACTGAAG CTAATCCGAA GATTATGATC 
CCAGAGGTAA TTGATACAAA AGCATCTGCT GAACTTGTAA GCAATTATGG GGTTGAGTCA 
TATCAGGAAA CCAGTTTTGA AATGTCAAAG TTAGTCCATT ATGCTGAAGA TAATGTGGCT 
GAAGAGGGAG CTGGAAAACA GCTTCAGTCT TTTGATATTA AGTTGAAGGC AGAGTTACAT 
TGCGATCCTT ATGGCATGGA TGTAAATCTT GCAAACAAAG ATATGGAGGG CATGGTGATT 
TTTGAAAAGT CACCTGGAGA TACTGTGGAA GATTATATCG GGGTTCCTCT CCATACAGAG 
GTTGAAGCAC TGGGAGCAAC AACTGAGAAG AGGTATACAG ATCCTGGAAC TTCACCAGAT 
TCAGAAGTTA TCAATTTAGT TGCCGAGGGC CAAGTCGATG CACGATGTCC AGAAGATTTT 
CATGATGCTG TTCTGTCTTC TTCTAAGGCT TTTGCTACAG ATCAAGGGGG TAATGGTAAA 
AGGAGGGGAA AAAAGAAAGA AAGGCTCCCT CAAGCCGCTA ACTGTTCACC TGCTGCGGCA 
AGCTTAAACA AAGTTAAATT GGCAAAGAAA CGTGGGGGCA GGCAGAGAAA GGCTGATGGC 
CTTTCCTCTA CTGAGATTCT TACTTCATCC AGCAGTGTGA ATGGTTTGAT CAACACACCA 
AGCAGTAAAG AATGTTCCGC AGAACAGGTA CCTTTGTCAA GAGAGACTGA ACTTGAAGTC 
TCTGGAGAGG TTTTGACAGA AGAAATCAGT ATGGAAACTA AAATATGTGT TGGTGGACTG 
GATGCTGAGC TTAGGTCATC AGAATCACAG ATTTCAAAGA ATCCTCTTCC TTCAACTAAA 
TCCAGGGGAA ATGGAGTGAA CAAGGGAAGG TCCAAAGTCT CCGACTCTGC AAAAAGCCGG 
AGGGCAAATG GTTGCAAGGA TAGAGGAAAC GATAGGAAGT CAGTTAAAAA GAACAAAGCC 
AAGGGGAAGA GTGTTTGTGA TCATGTTTTT TACAAAGTTG ATGATGATCC AGAAATAGGT 
CAAGAAGCAT TCTGCTCTGA CATGAAGTAT CGCTGTAATC ATACTGCAGA TGAAAATGGA 
AAAATTGATG CTGTTGAGGA TACTGCTGCA GAAGAGGTAG CTGATTTGGA CACGCCGTCA 
AGTGGTGTAA TGGAGCAAAA TTTATCCCCA GATAATGCTT GGGTTCGCTG TGATGATTGT 
CTTAAATGGC GGCGTATTCC AGTTCGACTT GTAGAATCTA TTAGTCAAAC ACATTGCCAA 
TGGATCTGCA AGGACAACAT GAATAAAGCC TTTGCTGATT GCTCCTTCCC CCAAGAGAAG 
TCAAATGCAG AAATAAATGC AGAGTTGGGC ATATCAGATG TTGATGAGGA TGGTTGCGAT 
GCCCCTTCAA ATTATATGGA ATTGGAATGT AGACAGACAT CAGTTTCCAA GGAGTATGAA 
TTTACACGCA TCACTACCAA TCAGTTTCTG CACCGTAGCC GTAAAACTCA AACTATTGAT 
GAGATCATGG TTTGTTACTG CAAAGCACCT GTGGCCGGTC GGTTAGGTTG TGGAGATGAA 
TGCTTGAATC GAATGCTTAA TATTGAGTGT GTTCAAGGAA CCTGTCCATG TGGGGACCAT 
TGCTCAAATC AACAGTTCCA AAAGCGCAAT TATGCCAAAA TGACATGGGA GCGATGTGGG 
AAGAAAGGTT TTGGACTGCG ATTGGATGAG GATATATCCA GAGGGCAATT CCTTATTGAA 
TACGTTGGAG AGGTGCTTGA TGTGCATGCT TATGAAGCAA GGCAAAAAGA TTATGCTTCC 
AAGGGTCACA AACATTTCTA CTTCATGACA TTGGATGGCA GTGAGGTAAT TGACGCATGT 
GCGAAGGGAA ATTTGGGGCG TTTCATTAAT CATAGTTGTG ATCCTAATTG TCGTACTGAA 
AAGTGGGTGG TCAATGGAGA AATCTGTATT GGATTATTTG CGTTGAGGGA CATTAAAATG 
GGCGAAGAGG TGACATTTGA CTATAACTAT GTAAGAGTTG TTGGGGCTGC TGCTAAAAGA 
TGCTATTGTG GTTCGCCTCA ATGTCGAGGC TATATTGGTG GTGATCCAAC TAGCACTGAA 
GTAGTTGATC AAGTTGATTC GGATGAAGAA TTTCCTGAAC CTGTAATGCT TGAAGATGGA 
AGAGTGGGAG GTGGCTTAAA AAATAAAATA TCCAAAACCA ATTTCTTTGG TTTGTCAAAA 
GACAGGGAAA TAGAATTCAA AACAGCTGTT GGGAACTTGG AGGTTGCAAC TGAAATTAAG 
GACTTGACAA GCCAATTAAC CCCTGCCATG TCTCTATCAC CCAGTGCATC AGAAATGAAT 
GGTTTACCTG GAGATTTTTC TTCTTCCAGT CAACAAGTAG AAACCTCCCC AAAGGCTGAA 
GATGTAATGA GCCAACCCAC ACCTGCTGTT CAGCAAGAGA TTTCCATGGA AGAGACTATG 
AACAAATCCT TGTATTCCAG TGAAAAGTTG AGGACCTCTC CAACTTCAAC TCCCACCAAA 
ATATTGCCTG ATGATGTTAT GATTAACAGG AAGTCTAAGT CTGCCGCAGC TGAAAACAAG 
AGGGTTTTTG TGAAATCTCG TTTTATTATT AAAACTCCAC ACCAATCTAG TTTAATCAAG 
AAAGGAAAGT CTGCTGGTAA TCTCATAAAC ATAAACAAGG TTCAGACAAT AGCCAGCAAA 
CCTCAGTTTC CACTTATCAA ACCCAAAAAA TTAATAGAGA GTACCTCGAA TGGCCATTTT 
GAAGCAGTTC AGGAGAAACT TAATGAGTTG TTGGATTCCG AGGGTGGCAT AAGCAAACGG 
AAAGATGCTC CTAAAGGCTA CCTGAAACTT CTCCTCCTTA CTGCAGCTTC TGGTGCTATT 
AGAAGTGGTG AAGCAATTCA GAGTAATCGG GAGCTTTCAA TGATACTCGA TGCACTCTTG 
AAAACTAGAT CACGAGTGGT ACTGATGGAT ATAATTAACA AAAATGGTTT AAGAATGCTT 
CACAACATAA TGAAGCAGTA CAGAAGGGAC TTTAAAAAGA TTCCAATTCT CAGGAAGCTT 
TTAAAGGTTT TAGAGCATTT GGCAGTCAGG GAGATACTTA CATTGGAACA TATAAGTGGA 
GGTCCTCCTT GTCCTGGAAT GGAGAGCTTT ACAGAATCCA TGTTGTCCCT GACAGAGCAT 
GATGACAAAC AGGTTCATCA AATTGCACGA AGTTTTCGAG ATAGATGGAT CCCTAGACAT 
ATCAGAAAGC ATAGCTATAT GGATAGGGAT GATGGGAGGA TGGAGATTCA CAGAGGTTCA 
AACTGCAATA GGGTTTCAGC TTCTCATAAT CATTGGCATG ATCAGGGTGT AAGACACACA 
GAAGCACTTA ATGGTGTTGT GGAATCAAAC CTTGCAATGA CTTCAGGGGG CACTGCTGTC 
CATGAGGACA ACTCTGTGAA TCGTGTAGGC AGCGGTGCAA GAACTCGCAA GCGTAAAACT 
AGATGGGATC AACCCGCTGT GGGAAATATA GCTTCCAGCT CTCTTCAGCA TATTAAACAG 
AATGTTAATT CTGGGTTGGT ACAACAATAT GAATCTAACC CATTACTTGA GTTAAGTAAA 
GAAGTGCCAG TTCATGTGGA CAAGGCAGGT AGAGAGTACA GTTATTGCCC TCATTGTGTT 
CGCAATTATC GCTGGCAAGA TGAATCTAGT AGTGCTGATG ACAGAAAGCA GAATATTCAT 
GAGGATGTTC CACCTGGATT CTCATCTCCG ATTAATGCTG CTCTTGCATC CAATGCTTCT 
TCCACTGTTG CTGACCCCCC TCAACAAAAT GTTTTCCATT TGAAGTTTCC TGTTGGTATG 
GTTGTCGGTC ATCCACAGAA GAAATTCAAT TCCCGCTTTC CTGTCTCGTA TGGAATTCCG 
TTGTCTGTTA TGCAGCAATT AGGATCACCC CTGGCTGAAA CTGTAGAGAG TTGGGTTATT 
GCTCCTGGCA TGCCTTTCCA TCCTTTTCCA CCATTACCAC CACTTCCCTC CTGTAAGAAA 
GGGACTCAAC CTTCTTGTGC TGTGAGCTCT ATGGAGGTTG ATGGTGAAGC AGATCGAGGG 
CAACAGGACA GCCATGATCC TACCACTTGT CCAAATGAAA GTAGTCCAAG CATGAGTGGT 
GCTAACCAAC CAGATGTGAA TAGCCCTTGT CCAAATGACC ATCAAACATT TAAACGTGCG 
AGGGGATTTT CATATGATTT GGGAAGGAGG TACTTTAAGC AACAGAAGTG GAATAAAGTG 
TCTCCTCCAT GGGTTCGGAA TAGGAATGGG TGGGGATGTG TAGGAGACAA CTCAAGAGGT 
GGAATGTGCA GCACTGACAT GGGAAGTCTA ACAAATGAAC AGAGGAACTC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome of black cottonwood, Populus trichocarpa (Torr. & Gray).
Science (New York, N.Y.)313(5793)1596-604(2006 Sep)
Tuskan GA,Difazio S,Jansson S,Bohlmann J,Grigoriev I,Hellsten U,Putnam N,Ralph S,Rombauts S,Salamov A,Schein J,Sterck L,Aerts A,Bhalerao RR,Bhalerao RP,Blaudez D,Boerjan W,Brun A,Brunner A,Busov V,Campbell M,Carlson J,Chalot M,Chapman J,Chen GL,Cooper D,Coutinho PM,Couturier J,Covert S,Cronk Q,Cunningham R,Davis J,Degroeve S,D?jardin A,Depamphilis C,Detter J,Dirks B,Dubchak I,Duplessis S,Ehlting J,Ellis B,Gendler K,Goodstein D,Gribskov M,Grimwood J,Groover A,Gunter L,Hamberger B,Heinze B,Helariutta Y,Henrissat B,Holligan D,Holt R,Huang W,Islam-Faridi N,Jones S,Jones-Rhoades M,Jorgensen R,Joshi C,Kangasj?rvi J,Karlsson J,Kelleher C,Kirkpatrick R,Kirst M,Kohler A,Kalluri U,Larimer F,Leebens-Mack J,Lepl? JC,Locascio P,Lou Y,Lucas S,Martin F,Montanini B,Napoli C,Nelson DR,Nelson C,Nieminen K,Nilsson O,Pereda V,Peter G,Philippe R,Pilate G,Poliakov A,Razumovskaya J,Richardson P,Rinaldi C,Ritland K,Rouz? P,Ryaboy D,Schmutz J,Schrader J,Segerman B,Shin H,Siddiqui A,Sterky F,Terry A,Tsai CJ,Uberbacher E,Unneberg P,Vahala J,Wall K,Wessler S,Yang G,Yin T,Douglas C,Marra M,Sandberg G,Van de Peer Y,Rokhsar D

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LOC18099600 cDNA ORF clone, Populus trichocarpa(black cottonwood)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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