TRHDE cDNA ORF clone, Pongo abelii(Sumatran orangutan)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRHDE
Entrez Gene ID	100460431
Full Name	thyrotropin releasing hormone degrading enzyme
Gene Type	protein-coding
Organism	Pongo abelii(Sumatran orangutan)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002823522.3	XP_002823568.2	LOW QUALITY PROTEIN: thyrotropin-releasing hormone-degrading ectoenzyme
XM_002823522.4	XP_002823568.3	thyrotropin-releasing hormone-degrading ectoenzyme

Pathways from BioSystems
Gene Ontology

The following TRHDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRHDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSa168244	XM_002823522.4 Latest version!	Pongo abelii thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OSa35238	XM_002823522.3 Latest version!	Pongo abelii thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRHDE ( XM_002823522.4 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_002823568.3 Pongo abelii thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.

Price & Availability↑

CloneID	OSa168244
Clone ID Related Accession (Same CDS sequence)	XM_002823522.4
Accession Version	XM_002823522.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-02-27
Organism	Pongo abelii(Sumatran orangutan)
Product	thyrotropin-releasing hormone-degrading ectoenzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 28, 2018 this sequence version replaced XM_002823522.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pongo abelii Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCGGCAGGG GGCTCTCAGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGACACA TCGCGGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCCGTGTC CCTCGTGGCA CTGCTCGCGG TCACAATGCT TGCCGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
GCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCCCG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGC AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CTGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTGGCCGG TTTTTTCCTC TACCCGCAAA CTCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCTCTC 
ATCGAGAATG AGCTCCTGGG CTTTTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ACGGATGGGT TACGGATCAT 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CGGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGC 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCAGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA ACATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAAA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTTGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCAGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGGGCT GGCTATTTGC CTCAGAATAT TCCTCTGGAG 
ATTATCAGAT ACCTGTCTGA GGAGAAGGAT TTTCTTCCTT GGCATGCTGC CAGCCGAGCT 
CTTTATCCTC TAGATAAATT ACTGGACCGC ATGGAAAACT ACAACGTTTT CAATGAATAT 
ATTTTAAAGC AAGTTGCAAC AATGTATATC AAGCTTGGGT GGCCGAAAAA TAATTTTAAT 
GGATCTCTTG TTCAAGCATC CTACCAACAT GAAGAACTAC GTAGAGAAGT TATAATGCTG 
GCCTGCAGTT TTGGCAACAA GCACTGTCAC CAACAGGCAT CAACACTTAT TTCAGATTGG 
ATTTCCAGCA ACAGGAACAG AATACCACTA AATGTTAGAG ACATCGTATA CTGTACAGGA 
GTGTCACTAC TGGATGAGGA TGTCTGGGAA TTCATATGGA TGAAATTCCA TTCCACCACA 
GCAGTTTCTG AGAAGAAAAT ATTATTGGAA GCCTTAACTT GCAGTGATGA CAGGAATTTA 
TTAAACAGGC TTCTAAATCT GTCACTGAAT TCTGAGGTGG TGCTGGATCA AGATGCAATT 
GATGTCATAA TCCATGTAGC TCGAAATCCA CATGGCCGAG ACCTTGCCTG GAAGTTTTTC 
AGGGATAAAT GGAAGATATT AAATACCAGG TATGGAGAAG CATTGTTTAT GAATTCCAAA 
CTCATCAGTG GTGTCACAGA ATTTCTTAAT ACTGAAGGTG AACTCAAAGA GCTCAAGAAC 
TTCATGAAGA ACTATGATGG GGTAGCTGCT GCTTCTTTCT CACGAGCTGT GGAAACTGTC 
GAAGCCAATG TGCGCTGGAA AATGCTTTAC CAAGACGAAC TTTTCCAATG GTTAGGAAAA 
GCTCTAAGAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002823568.3
CDS	92..3166
Translation	MGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPAGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWQPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNVFNEYILKQVATMYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

Target ORF information:

RefSeq Version	XM_002823522.4
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002823522.4

ORF Insert Sequence:

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCGGCAGGG GGCTCTCAGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGACACA TCGCGGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCCGTGTC CCTCGTGGCA CTGCTCGCGG TCACAATGCT TGCCGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
GCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCCCG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGC AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CTGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTGGCCGG TTTTTTCCTC TACCCGCAAA CTCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCTCTC 
ATCGAGAATG AGCTCCTGGG CTTTTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ACGGATGGGT TACGGATCAT 
TTTTCACAGA CCCCTCTCAT GTCCACATAT TATTTAGCCT GGGCAATTTG CAACTTCACA 
TACAGAGAAA CTACCACCAA GAGTGGGGTT GTAGTACGAT TATATGCAAG ACCTGATGCT 
ATCAGAAGAG GATCCGGGGA CTATGCTCTC CATATAACAA AGAGATTAAT AGAATTTTAT 
GAAGACTACT TTAAAGTGCC CTATTCCTTG CCAAAACTAG ATCTTTTAGC TGTGCCTAAG 
CATCCGTATG CTGCTATGGA GAACTGGGGA CTAAGTATTT TTGTGGAACA AAGAATACTG 
CTGGATCCCA GTGTTTCATC TATTTCTTAT TTGCTGGATG TCACCATGGT CATTGTTCAT 
GAGATATGTC ACCAGTGGTT TGGTGACCTT GTGACGCCTG TGTGGTGGGA AGACGTGTGG 
CTGAAGGAAG GGTTTGCTCA CTACTTTGAA TTTGTTGGTA CGGACTACCT CTATCCTGGC 
TGGAACATGG AAAAGCAGAG GTTTCTGACC GATGTTCTGC ATGAAGTGAT GCTGCTGGAC 
GGTTTGGCCA GTTCCCATCC AGTATCACAG GAAGTGCTGC AGGCAACAGA TATTGACAGG 
GTGTTTGACT GGATCGCATA TAAAAAGGGT GCTGCTTTAA TAAGAATGCT GGCTAATTTT 
ATGGGCCATT CAGTTTTCCA GAGGGGTTTG CAAGATTATT TAACCATTCA TAAGTATGGC 
AATGCAGCCA GAAATGATCT CTGGAATACA TTATCAGAGG CTTTAAAAAG AAATGGGAAA 
TATGTAAATA TACAAGAAGT AATGGATCAG TGGACACTCC AGATGGGTTA TCCTGTTATC 
ACCATCTTGG GAAACACAAC AGCAGAAAAT AGAATAATAA TTACCCAACA ACATTTTATC 
TATGATATCA GTGCTAAAAC TAAAGCACTT AAACTTCAAA ATAACAGTTA CCTGTGGCAG 
ATTCCATTAA CTATTGTTGT AGGAAATAGA AGCCATGTGT CTTCAGAAGC AATTATTTGG 
GTGTCTAACA AATCAGAGCA CCACAGAATA ACTTATTTGG ACAAAGGAAG CTGGCTGCTG 
GGGAACATCA ATCAAACTGG CTATTTTAGA GTCAACTATG ACCTAAGGAA CTGGAGATTA 
TTAATTGATC AATTAATCCG GAATCATGAG GTTCTTTCTG TCAGTAACCG AGCAGGCTTG 
ATCGATGATG CCTTCAGCCT AGCCAGGGCT GGCTATTTGC CTCAGAATAT TCCTCTGGAG 
ATTATCAGAT ACCTGTCTGA GGAGAAGGAT TTTCTTCCTT GGCATGCTGC CAGCCGAGCT 
CTTTATCCTC TAGATAAATT ACTGGACCGC ATGGAAAACT ACAACGTTTT CAATGAATAT 
ATTTTAAAGC AAGTTGCAAC AATGTATATC AAGCTTGGGT GGCCGAAAAA TAATTTTAAT 
GGATCTCTTG TTCAAGCATC CTACCAACAT GAAGAACTAC GTAGAGAAGT TATAATGCTG 
GCCTGCAGTT TTGGCAACAA GCACTGTCAC CAACAGGCAT CAACACTTAT TTCAGATTGG 
ATTTCCAGCA ACAGGAACAG AATACCACTA AATGTTAGAG ACATCGTATA CTGTACAGGA 
GTGTCACTAC TGGATGAGGA TGTCTGGGAA TTCATATGGA TGAAATTCCA TTCCACCACA 
GCAGTTTCTG AGAAGAAAAT ATTATTGGAA GCCTTAACTT GCAGTGATGA CAGGAATTTA 
TTAAACAGGC TTCTAAATCT GTCACTGAAT TCTGAGGTGG TGCTGGATCA AGATGCAATT 
GATGTCATAA TCCATGTAGC TCGAAATCCA CATGGCCGAG ACCTTGCCTG GAAGTTTTTC 
AGGGATAAAT GGAAGATATT AAATACCAGG TATGGAGAAG CATTGTTTAT GAATTCCAAA 
CTCATCAGTG GTGTCACAGA ATTTCTTAAT ACTGAAGGTG AACTCAAAGA GCTCAAGAAC 
TTCATGAAGA ACTATGATGG GGTAGCTGCT GCTTCTTTCT CACGAGCTGT GGAAACTGTC 
GAAGCCAATG TGCGCTGGAA AATGCTTTAC CAAGACGAAC TTTTCCAATG GTTAGGAAAA 
GCTCTAAGAC ACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_002823522.3 ) cDNA ORF clone, Pongo abelii(Sumatran orangutan) -> XP_002823568.2 Pongo abelii thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA.

Price & Availability↑

CloneID	OSa35238
Clone ID Related Accession (Same CDS sequence)	XM_002823522.3
Accession Version	XM_002823522.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-09-22
Organism	Pongo abelii(Sumatran orangutan)
Product	LOW QUALITY PROTEIN: thyrotropin-releasing hormone-degrading ectoenzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_002885935.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 23, 2014 this sequence version replaced XM_002823522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pongo abelii Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCGGCAGGG GGCTCTCAGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGACACA TCGCGGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCCGTGTC CCTCGTGGCA CTGCTCGCGG TCACAATGCT TGCCGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
GCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCCCG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGC AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CTGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTGGCCGG TTTTTTCCTC TACCCGCAAA CTCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCTCTC 
ATCGAGAATG AGCTCCTGGG CTTTTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ACGGATGGGT TACGGATCAT 
TTTTCACAGA CCCCTCTCAT GTCCATATTA TTNNNNNNNN NNACATATTA TTTAGCCTGG 
GCAATTTGCA ACTTCACATA CAGAGAAACT ACCACCAAGA GTGGGGTTGT AGTACGATTA 
TATGCAAGAC CTGATGCTAT CAGAAGAGGA TCCGGGGACT ATGCTCTCCA TATAACAAAG 
AGATTAATAG AATTTTATGA AGACTACTTT AAAGTGCCCT ATTCCTTGCC AAAACTAGAT 
CTTTTAGCTG TGCCTAAGCA TCCGTATGCT GCTATGGAGA ACTGGGGACT AAGTATTTTT 
GTGGAACACC CCGCCCCGCC GCCCCGCAGT GTTTCATCTA TTTCTTATTT GCTGGATGTC 
ACCATGGTCA TTGTTCATGA GATATGTCAC CAGTGGTTTG GTGACCTTGT GACGCCTGTG 
TGGTGGGAAG ACGTGTGGCT GAAGGAAGGG TTTGCTCACT ACTTTGAATT TGTTGGTACG 
GACTACCTCT ATCCTGGCTG GAACATGGAA AAGCAGAGGT TTCTGACCGA TGTTCTGCAT 
GAAGTGATGC TGCTGGACGG TTTGGCCAGT TCCCATCCAG TATCACAGGA AGTGCTGCAG 
GCAACAGATA TTGACAGGGT GTTTGACTGG ATCGCATATA AAAAGGGTGC TGCTTTAATA 
AGAATGCTGG CTAATTTTAT GGGCCATTCA GTTTTCCAGA GGGGTTTGCA AGATTATTTA 
ACCATTCATA AGTATGGCAA TGCAGCCAGA AATGATCTCT GGAATACATT ATCAGAGGCT 
TTAAAAAGAA ATGGGAAATA TGTAAATATA CAAGAAGTAA TGGATCAGTG GACACTCCAG 
ATGGGTTATC CTGTTATCAC CATCTTGGGA AACACAACAG CAGAAAATAG AATAATAATT 
ACCCAACAAC ATTTTATCTA TGATATCAGT GCTAAAACTA AAGCACTTAA ACTTCAAAAT 
AACAGTTACC TGTGGCAGAT TCCATTAACT ATTGTTGTAG GAAATAGAAG CCATGTGTCT 
TCAGAAGCAA TTATTTGGGT GTCTAACAAA TCAGAGCACC ACAGAATAAC TTATTTGGAC 
AAAGGAAGCT GGCTGCTGGG GAACATCAAT CAAACTGGCT ATTTTAGAGT CAACTATGAC 
CTAAGGAACT GGAGATTATT AATTGATCAA TTAATCCGGA ATCATGAGGT TCTTTCTGTC 
AGTAACCGAG CAGGCTTGAT CGATGATGCC TTCAGCCTAG CCAGGGCTGG CTATTTGCCT 
CAGAATATTC CTCTGGAGAT TATCAAATAC CTGTCTGAGG AGAAGGATTT TCTTCCTTGG 
CATGCTGCCA GCCGAGCTCT TTATCCTCTA GATAAATTAC TGGACCGCAT GGAAAACTAC 
AACGTTTTCA ATGAATATAT TTTAAAGCAA GTTGCAACAA TGTATATCAA GCTTGGGTGG 
CCGAAAAATA ATTTTAATGG ATCTCTTGTT CAAGCATCCT ACCAACATGA AGAACTACGT 
AGAGAAGTTA TAATGCTGGC CTGCAGTTTT GGCAACAAGC ACTGTCACCA ACAGGCATCA 
ACACTTATTT CAGATTGGAT TTCCAGCAAC AGGAACAGAA TACCACTAAA TGTTAGAGAC 
ATCGTATACT GTACAGGAGT GTCACTACTG GATGAGGATG TCTGGGAATT CATATGGATG 
AAATTCCATT CCACCACAGC AGTTTCTGAG AAGAAAATAT TATTGGAAGC CTTAACTTGC 
AGTGATGACA GGAATTTATT AAACAGGCTT CTAAATCTGT CACTGAATTC TGAGGTGGTG 
CTGGATCAAG ATGCAATTGA TGTCATAATC CATGTAGCTC GAAATCCACA TGGCCGAGAC 
CTTGCCTGGA AGTTTTTCAG GGATAAATGG AAGATATTAA ATACCAGGTA TGGAGAAGCA 
TTGTTTATGA ATTCCAAACT CATCAGTGGT GTCACAGAAT TTCTTAATAC TGAAGGTGAA 
CTCAAAGAGC TCAAGAACTT CATGAAGAAC TATGATGGGG TAGCTGCTGC TTCTTTCTCA 
CGAGCTGTGG AAACTGTCGA AGCCAATGTG CGCTGGAAAA TGCTTTACCA AGACGAGCTT 
TTCCAATGGT TAGGAAAAGC TCTAAGACAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002823568.2
CDS	92..3184
Translation	MGEDDAALRAGGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSLVALLAVTMLAVLLSLRFDECGASATPGADGGPAGFPERGGNGSLPGSARRNHHAGGDSWQPEAGGVASPGTPSAQPPSEEEREPWQPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQLAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRNYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSILnullnullnullnullTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEHPAPPPRSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALKRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRIIITQQHFIYDISAKTKALKLQNNSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRITYLDKGSWLLGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAGLIDDAFSLARAGYLPQNIPLEIIKYLSEEKDFLPWHAASRALYPLDKLLDRMENYNVFNEYILKQVATMYIKLGWPKNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKNYDGVAAASFSRAVETVEANVRWKMLYQDELFQWLGKALRH

Target ORF information:

RefSeq Version	XM_002823522.3
Organism	Pongo abelii(Sumatran orangutan)
Definition	Pongo abelii thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002823522.3

ORF Insert Sequence:

ATGGGGGAAG ACGACGCCGC GCTTCGGGCT GGCGGCAGGG GGCTCTCAGA CCCGTGGGCA 
GACTCAGTGG GAGTGCGACC CCGCACCACG GAGCGACACA TCGCGGTACA CAAGCGGCTT 
GTGCTGGCCT TCGCCGTGTC CCTCGTGGCA CTGCTCGCGG TCACAATGCT TGCCGTGCTG 
CTCAGCCTGC GCTTCGACGA GTGCGGGGCG AGTGCCACGC CAGGCGCCGA CGGTGGCCCC 
GCAGGCTTTC CGGAGCGCGG CGGCAACGGG AGCCTCCCTG GATCGGCCCG GCGCAACCAC 
CACGCAGGCG GGGACTCCTG GCAGCCCGAG GCGGGTGGGG TGGCCAGTCC GGGGACCCCG 
TCGGCCCAGC CGCCGTCGGA GGAGGAGCGG GAGCCGTGGC AGCCGTGGAC GCAGCTGCGC 
CTGTCGGGCC ACCTGAAGCC GCTGCACTAC AATCTGATGC TCACCGCCTT CATGGAGAAC 
TTCACCTTCT CTGGGGAGGT CAACGTGGAG ATCGCGTGCC GGAACGCCAC CCGCTACGTA 
GTGCTGCACG CTTCCCGAGT GGCGGTGGAG AAAGTGCAGC TGGCCGAGGA CCGGGCGTTC 
GGGGCTGTCC CTGTGGCCGG TTTTTTCCTC TACCCGCAAA CTCAGGTCTT AGTGGTGGTG 
CTGAATAGGA CACTGGACGC GCAGAGGAAT TACAATCTGA AGATTATCTA CAACGCTCTC 
ATCGAGAATG AGCTCCTGGG CTTTTTCCGC AGCTCCTATG TGCTCCACGG GGAGAGAAGA 
TTCCTTGGTG TTACTCAGTT TTCGCCTACA CATGCCAGAA AGGCATTTCC TTGTTTTGAT 
GAGCCAATCT ACAAGGCTAC TTTCAAAATC AGCATCAAGC ATCAAGCAAC CTATTTATCT 
TTATCTAATA TGCCAGTGGA AACTTCCGTG TTTGAGGAAG ACGGATGGGT TACGGATCAT 
TTTTCACAGA CCCCTCTCAT GTCCATATTA TTNNNNNNNN NNACATATTA TTTAGCCTGG 
GCAATTTGCA ACTTCACATA CAGAGAAACT ACCACCAAGA GTGGGGTTGT AGTACGATTA 
TATGCAAGAC CTGATGCTAT CAGAAGAGGA TCCGGGGACT ATGCTCTCCA TATAACAAAG 
AGATTAATAG AATTTTATGA AGACTACTTT AAAGTGCCCT ATTCCTTGCC AAAACTAGAT 
CTTTTAGCTG TGCCTAAGCA TCCGTATGCT GCTATGGAGA ACTGGGGACT AAGTATTTTT 
GTGGAACACC CCGCCCCGCC GCCCCGCAGT GTTTCATCTA TTTCTTATTT GCTGGATGTC 
ACCATGGTCA TTGTTCATGA GATATGTCAC CAGTGGTTTG GTGACCTTGT GACGCCTGTG 
TGGTGGGAAG ACGTGTGGCT GAAGGAAGGG TTTGCTCACT ACTTTGAATT TGTTGGTACG 
GACTACCTCT ATCCTGGCTG GAACATGGAA AAGCAGAGGT TTCTGACCGA TGTTCTGCAT 
GAAGTGATGC TGCTGGACGG TTTGGCCAGT TCCCATCCAG TATCACAGGA AGTGCTGCAG 
GCAACAGATA TTGACAGGGT GTTTGACTGG ATCGCATATA AAAAGGGTGC TGCTTTAATA 
AGAATGCTGG CTAATTTTAT GGGCCATTCA GTTTTCCAGA GGGGTTTGCA AGATTATTTA 
ACCATTCATA AGTATGGCAA TGCAGCCAGA AATGATCTCT GGAATACATT ATCAGAGGCT 
TTAAAAAGAA ATGGGAAATA TGTAAATATA CAAGAAGTAA TGGATCAGTG GACACTCCAG 
ATGGGTTATC CTGTTATCAC CATCTTGGGA AACACAACAG CAGAAAATAG AATAATAATT 
ACCCAACAAC ATTTTATCTA TGATATCAGT GCTAAAACTA AAGCACTTAA ACTTCAAAAT 
AACAGTTACC TGTGGCAGAT TCCATTAACT ATTGTTGTAG GAAATAGAAG CCATGTGTCT 
TCAGAAGCAA TTATTTGGGT GTCTAACAAA TCAGAGCACC ACAGAATAAC TTATTTGGAC 
AAAGGAAGCT GGCTGCTGGG GAACATCAAT CAAACTGGCT ATTTTAGAGT CAACTATGAC 
CTAAGGAACT GGAGATTATT AATTGATCAA TTAATCCGGA ATCATGAGGT TCTTTCTGTC 
AGTAACCGAG CAGGCTTGAT CGATGATGCC TTCAGCCTAG CCAGGGCTGG CTATTTGCCT 
CAGAATATTC CTCTGGAGAT TATCAAATAC CTGTCTGAGG AGAAGGATTT TCTTCCTTGG 
CATGCTGCCA GCCGAGCTCT TTATCCTCTA GATAAATTAC TGGACCGCAT GGAAAACTAC 
AACGTTTTCA ATGAATATAT TTTAAAGCAA GTTGCAACAA TGTATATCAA GCTTGGGTGG 
CCGAAAAATA ATTTTAATGG ATCTCTTGTT CAAGCATCCT ACCAACATGA AGAACTACGT 
AGAGAAGTTA TAATGCTGGC CTGCAGTTTT GGCAACAAGC ACTGTCACCA ACAGGCATCA 
ACACTTATTT CAGATTGGAT TTCCAGCAAC AGGAACAGAA TACCACTAAA TGTTAGAGAC 
ATCGTATACT GTACAGGAGT GTCACTACTG GATGAGGATG TCTGGGAATT CATATGGATG 
AAATTCCATT CCACCACAGC AGTTTCTGAG AAGAAAATAT TATTGGAAGC CTTAACTTGC 
AGTGATGACA GGAATTTATT AAACAGGCTT CTAAATCTGT CACTGAATTC TGAGGTGGTG 
CTGGATCAAG ATGCAATTGA TGTCATAATC CATGTAGCTC GAAATCCACA TGGCCGAGAC 
CTTGCCTGGA AGTTTTTCAG GGATAAATGG AAGATATTAA ATACCAGGTA TGGAGAAGCA 
TTGTTTATGA ATTCCAAACT CATCAGTGGT GTCACAGAAT TTCTTAATAC TGAAGGTGAA 
CTCAAAGAGC TCAAGAACTT CATGAAGAAC TATGATGGGG TAGCTGCTGC TTCTTTCTCA 
CGAGCTGTGG AAACTGTCGA AGCCAATGTG CGCTGGAAAA TGCTTTACCA AGACGAGCTT 
TTCCAATGGT TAGGAAAAGC TCTAAGACAC TAA