gcsh cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	gcsh
Entrez Gene ID	103462489
Full Name	glycine cleavage system protein H
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008405326.2	XP_008403548.1	glycine cleavage system H protein, mitochondrial

Pathways from BioSystems
Gene Ontology

The following gcsh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gcsh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo190906	XM_008405326.2 Latest version!	Poecilia reticulata glycine cleavage system protein H (gcsh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gcsh ( XM_008405326.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008403548.1 Poecilia reticulata glycine cleavage system protein H (gcsh), mRNA.

Price & Availability↑

CloneID	OPo190906
Clone ID Related Accession (Same CDS sequence)	XM_008405326.2
Accession Version	XM_008405326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	glycine cleavage system H protein, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008405326.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGATGA GAGCAGCGCT GCGGTGCCTT TCTGTAAACT TTTCTCCCAG GCTGCCGCAA 
CTCTCGTCCG CAGCGCAAGT TTCAGCGACT CGGCTGCTGT GCCGGAGCGG CGCCCCGCGG 
ACTCTGAGCA CAACATCGGG CCAGTGCACA GCCCTCAAGT TCACCGAGAA GCATGAGTGG 
GTGCTGGTGG AGAGTGGAAT CGGCACTGTG GGAATCAGTA ACTATGCTCA GGAAGCTTTA 
GGTGACGTGG TTTACTGTGG ACTGCCCGAG GTTGGCCAAA AACTTGAACA GCTGGAGGAG 
TTTGGTGCCT TGGAAAGTGT AAAGGCTGCC AGTGAGCTGT ACTCACCACT GACAGGGGAA 
GTGACAGCAA TCAACACGGA GCTGGCCGAA AATCCAGGAC TCGTGAATAA ATCCTGCTAC 
GCAGACGGAT GGCTAATCAA GATGACCATC GAAAAGCCTG AAGAACTCGA TAAGCTCATG 
GACCAACCTG CCTATGATAC ATTTATTAAG TCGCTGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008403548.1
CDS	79..603
Translation	MAMRAALRCLSVNFSPRLPQLSSAAQVSATRLLCRSGAPRTLSTTSGQCTALKFTEKHEWVLVESGIGTVGISNYAQEALGDVVYCGLPEVGQKLEQLEEFGALESVKAASELYSPLTGEVTAINTELAENPGLVNKSCYADGWLIKMTIEKPEELDKLMDQPAYDTFIKSLEE

Target ORF information:

RefSeq Version	XM_008405326.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata glycine cleavage system protein H (gcsh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008405326.2

ORF Insert Sequence:

ATGGCGATGA GAGCAGCGCT GCGGTGCCTT TCTGTAAACT TTTCTCCCAG GCTGCCGCAA 
CTCTCGTCCG CAGCGCAAGT TTCAGCGACT CGGCTGCTGT GCCGGAGCGG CGCCCCGCGG 
ACTCTGAGCA CAACATCGGG CCAGTGCACA GCCCTCAAGT TCACCGAGAA GCATGAGTGG 
GTGCTGGTGG AGAGTGGAAT CGGCACTGTG GGAATCAGTA ACTATGCTCA GGAAGCTTTA 
GGTGACGTGG TTTACTGTGG ACTGCCCGAG GTTGGCCAAA AACTTGAACA GCTGGAGGAG 
TTTGGTGCCT TGGAAAGTGT AAAGGCTGCC AGTGAGCTGT ACTCACCACT GACAGGGGAA 
GTGACAGCAA TCAACACGGA GCTGGCCGAA AATCCAGGAC TCGTGAATAA ATCCTGCTAC 
GCAGACGGAT GGCTAATCAA GATGACCATC GAAAAGCCTG AAGAACTCGA TAAGCTCATG 
GACCAACCTG CCTATGATAC ATTTATTAAG TCGCTGGAGG AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.