LOC103146077 cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC103146077
Entrez Gene ID	103146077
Full Name	rho guanine nucleotide exchange factor 18-like
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007563811.2	XP_007563873.1	rho guanine nucleotide exchange factor 18-like isoform X1
XM_016677253.1	XP_016532739.1	rho guanine nucleotide exchange factor 18-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC103146077 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC103146077 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo146467	XM_007563811.2 Latest version!	Poecilia formosa rho guanine nucleotide exchange factor 18-like (LOC103146077), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPo146468	XM_016677253.1 Latest version!	Poecilia formosa rho guanine nucleotide exchange factor 18-like (LOC103146077), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC103146077 ( XM_007563811.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007563873.1 Poecilia formosa rho guanine nucleotide exchange factor 18-like (LOC103146077), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo146467
Clone ID Related Accession (Same CDS sequence)	XM_007563811.2
Accession Version	XM_007563811.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	rho guanine nucleotide exchange factor 18-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800090.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007563811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGA CTGAAGGCCA GCGGGTGAAG CCTTTCGCTG ATGAATCTGC GTTATTGGCT 
GAGCCAATCA ACCCAGAAGA CTCCCATTAT GCTGAGCTGC GAGCTGATCT GGAGAGCGAC 
TCCCGCGGTC TGGAGGCGGA GTCCTGGAGC TCGGCTGTGG ACCAGAACTA CCTGATGTCT 
CTGAACAAAG AGGCTGTCAA AAGGCAGGAC GTGATCTACG AGTTGATCCA GACTGAGATG 
AACCATGTGC GAACCTTGAA AATCCTCCTC CACATCTACA TGTACGAGCT GAAGAAGTTC 
CAATCCATCA TCGACCTGGA GAAGCTCTTC CCCAGCCTGG AGGTTCTGCT CAGCCTCCAC 
TATGTCTTCC TCAAGTCCCT CAAATCCCGG AGAGATGAGA GTCGGGATGA GGACAATCCA 
AACAACTACT ACATCACAGA GCTGGCGGAT GTCCTCATCT ATCAGTTTTC AGGTAATCTG 
GGAATACAGA TGGCGACGGG TTACGGCGAC TTTTGCAGCC ATCACAGCGA AGCTGTCAGC 
TACTACAAGG AGCAAATGCA GAGCAACAAG ACGCTGCAAA TTTTGATTAG AAAAATCGAT 
CAGCTGCCTC TGGCGCGAAG ATTAACGATT CCTGAATGTT TCTTGCTGGT GACTCAACGC 
ATCACAAAAT ATCCGGTTCT GTTGGAGCGA ATCATCCAGA ACACAGACGC TGACACACAT 
GAGCACAGGC AGCTGGAGAA AGCTCTGGAG CTGATCAAGC ACACCATCTC CCAGGTGAAC 
ACCCAGGTGA GTGAATTTGG GAAGACCGCT CGCCTCAGAG AGATCATTAA TCGACTGGAG 
CAAAAGACTC AGGCCAAGCT GAAAGATGGC CGCGGGTTCT GGAGAGACGA CCTGAGGCAC 
GGCAACAGGA CGCTGGTGCA TGAAGGACCC CTGACCTGGA AGTTGTCCGG TAAACAAAAA 
GAAATTCATG CTGTGCTGCT GTCAGATATG CTGCTTCTGC TACAGGAAAA AGATCAGAAG 
CTTGTATTAG CTGCCATGGA TAGCAAACCG CCAGTCATCT ACCTGCAGGG AGTGATTGTG 
AGGGTAGTGG CCATGGAGGA AAGAGCCATG TACGTCATCT GTGCGTGTCC CTCCGGCTCG 
CCAGAAATGT ACGAGCTCCA CACGAGCTCC AAAGAGGAGT GCAAAACCTG GATGGACCTC 
ATACACAACA TGGCAGAAAG TTGCAGAGAC AATGAAGTGC ACAAAGAAAT GGTTACTAAA 
CTGCAGCAGT ATCAAGATAA GTTGCAGAAA AGAGACGAGC AGATAAAGAA AAGTCTGGAA 
AAGAAACAGG AAATTTTTGC TGAACTTTAT CATGACGTGT TGGGTCAGAA AAGCCTTTAC 
AACGGGCTGC TGCTGCGAGG CGATGCTTCT GACCTCCAGG AGGGGGAGAC CTTGTTGAAT 
GGGGCCATCG AAGAGGTGGA AAAGTTACAG ACCCTGCTCT TTATGATGAT TAAACATCCA 
GCCACTCCTG AGGGGGAAAT TGAGACACAG CAAAGTGTCA GGAGGGCACA GACCTTCAAA 
GGCTCTCTGG ATAATCTGAT AGCTAAAAGC ATGAAATACG AAGATGCAGC TGAGAAGGTG 
CAACGCAGAT ACAGCAGCTG TGACCTTCTG CTCAAGAACA TGTCGATCTC TGAAGACCTG 
GGGAAGTCTA CTGATGATGA TGATGATGAT GATGATGATG ATGAAAGTGG ATCCCACCCA 
AAGCTGCTCT GTGGTTCAGC CTTCGGTGGA TTCCCTGGGA CAGCGGTGTT TGATAGCGTT 
CAGTCGCTGG CGCAGAAGCT CTGCAGCTTA CAGGCCGTCA TCGCCCAGCA GGACAGCAGG 
ATCGAACTGC AGAATCTCTT CTGGTCAAAG AGCAAGCTGC CCGTCCGCAG CAACGCCAAC 
GCGCTGCTTG AGAAGGAGAA AGAGCGCAAC TTGGAGAAGC AGAGAGCAGA GCTGGCTAAG 
CTGCACAAGC AGCAGAACCA GCATCGAGCG GAGCAGCAGC GCTGGGAGAA AGAGAGACAG 
AAGCAGCAGT TGCTGATGGA AAGTCAGAAC GCCAAGATGC AGCAGCGGGA GGAGGAGTGC 
AAGATGATGG AGGAGAAACT AAAAGAAAAA GAGCTGAACC TGAAGCAGAG TCAGGAGACG 
TACCAACAGG ACCTGGAGAA TCTGAGGGAG ACCACACAGA AACTGGACAG GGAGTGGAAG 
ATGATTGAAC GGGAAAAGGA ACGTTTGGAA AAGATGAAGG CAAAGCTTGT CAACCATTAT 
GACGATCCGA CATGGTCTCC GGCGCTCTCC ACTGCCCCAT CATTCAGGAG CAGCATAGTA 
AACGGAGCAG GGACTCTGAC CCCAGTTCCC AATCCACTGG TCTTTACTAG TAACTCTCCT 
GACCCGGCAG AATCTCCTCC GCGGGTTCCT CCCCGGAGCA CCAGCATCAG AATGAGCAGG 
CAGATGCTGC CGGTTCAGTT GATGAGCACC ACCAACCAGG TGCAGACCCC TGTGGAGGTG 
CAGCAGAAGA TTCCCAGGAA GCTGGCGGCC GAACCCAAAA AGAAGGAGAA AACCAAGTCA 
AAGCGCTCCC ACAAGAGAGC CCACAGTGCA GCCAATATAG ATGTAACCCA GGTGATCCCG 
ATCCGGGTGA TGGGGAAAGA CAGTGGAAGT CTTAAAGCCC AAATGAGCAC AAGTCCAAAA 
AGAGTCTTAA GTGCAGACAC CTTCAGGACA CCAGGATCTG GTGACCTGAA GCCCAGATCC 
TGGTCACCAC CTTCAGGATC TGGTCGGCTT CCCAGAACGG CTTCCCAGAA CGTGAAGCCG 
TCTCAGTCGC TCATCGCACC GAAGTGGAAC AACGTTGGTT CTCTTCCACC GCGGCCTCCT 
CCCTTCCCGA CAGACATAAA TACGACAGAA CAAGAAAAGG TTATCGTTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007563873.1
CDS	523..3456
Translation	MDETEGQRVKPFADESALLAEPINPEDSHYAELRADLESDSRGLEAESWSSAVDQNYLMSLNKEAVKRQDVIYELIQTEMNHVRTLKILLHIYMYELKKFQSIIDLEKLFPSLEVLLSLHYVFLKSLKSRRDESRDEDNPNNYYITELADVLIYQFSGNLGIQMATGYGDFCSHHSEAVSYYKEQMQSNKTLQILIRKIDQLPLARRLTIPECFLLVTQRITKYPVLLERIIQNTDADTHEHRQLEKALELIKHTISQVNTQVSEFGKTARLREIINRLEQKTQAKLKDGRGFWRDDLRHGNRTLVHEGPLTWKLSGKQKEIHAVLLSDMLLLLQEKDQKLVLAAMDSKPPVIYLQGVIVRVVAMEERAMYVICACPSGSPEMYELHTSSKEECKTWMDLIHNMAESCRDNEVHKEMVTKLQQYQDKLQKRDEQIKKSLEKKQEIFAELYHDVLGQKSLYNGLLLRGDASDLQEGETLLNGAIEEVEKLQTLLFMMIKHPATPEGEIETQQSVRRAQTFKGSLDNLIAKSMKYEDAAEKVQRRYSSCDLLLKNMSISEDLGKSTDDDDDDDDDDESGSHPKLLCGSAFGGFPGTAVFDSVQSLAQKLCSLQAVIAQQDSRIELQNLFWSKSKLPVRSNANALLEKEKERNLEKQRAELAKLHKQQNQHRAEQQRWEKERQKQQLLMESQNAKMQQREEECKMMEEKLKEKELNLKQSQETYQQDLENLRETTQKLDREWKMIEREKERLEKMKAKLVNHYDDPTWSPALSTAPSFRSSIVNGAGTLTPVPNPLVFTSNSPDPAESPPRVPPRSTSIRMSRQMLPVQLMSTTNQVQTPVEVQQKIPRKLAAEPKKKEKTKSKRSHKRAHSAANIDVTQVIPIRVMGKDSGSLKAQMSTSPKRVLSADTFRTPGSGDLKPRSWSPPSGSGRLPRTASQNVKPSQSLIAPKWNNVGSLPPRPPPFPTDINTTEQEKVIVV

Target ORF information:

RefSeq Version	XM_007563811.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa rho guanine nucleotide exchange factor 18-like (LOC103146077), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007563811.2

ORF Insert Sequence:

ATGGATGAGA CTGAAGGCCA GCGGGTGAAG CCTTTCGCTG ATGAATCTGC GTTATTGGCT 
GAGCCAATCA ACCCAGAAGA CTCCCATTAT GCTGAGCTGC GAGCTGATCT GGAGAGCGAC 
TCCCGCGGTC TGGAGGCGGA GTCCTGGAGC TCGGCTGTGG ACCAGAACTA CCTGATGTCT 
CTGAACAAAG AGGCTGTCAA AAGGCAGGAC GTGATCTACG AGTTGATCCA GACTGAGATG 
AACCATGTGC GAACCTTGAA AATCCTCCTC CACATCTACA TGTACGAGCT GAAGAAGTTC 
CAATCCATCA TCGACCTGGA GAAGCTCTTC CCCAGCCTGG AGGTTCTGCT CAGCCTCCAC 
TATGTCTTCC TCAAGTCCCT CAAATCCCGG AGAGATGAGA GTCGGGATGA GGACAATCCA 
AACAACTACT ACATCACAGA GCTGGCGGAT GTCCTCATCT ATCAGTTTTC AGGTAATCTG 
GGAATACAGA TGGCGACGGG TTACGGCGAC TTTTGCAGCC ATCACAGCGA AGCTGTCAGC 
TACTACAAGG AGCAAATGCA GAGCAACAAG ACGCTGCAAA TTTTGATTAG AAAAATCGAT 
CAGCTGCCTC TGGCGCGAAG ATTAACGATT CCTGAATGTT TCTTGCTGGT GACTCAACGC 
ATCACAAAAT ATCCGGTTCT GTTGGAGCGA ATCATCCAGA ACACAGACGC TGACACACAT 
GAGCACAGGC AGCTGGAGAA AGCTCTGGAG CTGATCAAGC ACACCATCTC CCAGGTGAAC 
ACCCAGGTGA GTGAATTTGG GAAGACCGCT CGCCTCAGAG AGATCATTAA TCGACTGGAG 
CAAAAGACTC AGGCCAAGCT GAAAGATGGC CGCGGGTTCT GGAGAGACGA CCTGAGGCAC 
GGCAACAGGA CGCTGGTGCA TGAAGGACCC CTGACCTGGA AGTTGTCCGG TAAACAAAAA 
GAAATTCATG CTGTGCTGCT GTCAGATATG CTGCTTCTGC TACAGGAAAA AGATCAGAAG 
CTTGTATTAG CTGCCATGGA TAGCAAACCG CCAGTCATCT ACCTGCAGGG AGTGATTGTG 
AGGGTAGTGG CCATGGAGGA AAGAGCCATG TACGTCATCT GTGCGTGTCC CTCCGGCTCG 
CCAGAAATGT ACGAGCTCCA CACGAGCTCC AAAGAGGAGT GCAAAACCTG GATGGACCTC 
ATACACAACA TGGCAGAAAG TTGCAGAGAC AATGAAGTGC ACAAAGAAAT GGTTACTAAA 
CTGCAGCAGT ATCAAGATAA GTTGCAGAAA AGAGACGAGC AGATAAAGAA AAGTCTGGAA 
AAGAAACAGG AAATTTTTGC TGAACTTTAT CATGACGTGT TGGGTCAGAA AAGCCTTTAC 
AACGGGCTGC TGCTGCGAGG CGATGCTTCT GACCTCCAGG AGGGGGAGAC CTTGTTGAAT 
GGGGCCATCG AAGAGGTGGA AAAGTTACAG ACCCTGCTCT TTATGATGAT TAAACATCCA 
GCCACTCCTG AGGGGGAAAT TGAGACACAG CAAAGTGTCA GGAGGGCACA GACCTTCAAA 
GGCTCTCTGG ATAATCTGAT AGCTAAAAGC ATGAAATACG AAGATGCAGC TGAGAAGGTG 
CAACGCAGAT ACAGCAGCTG TGACCTTCTG CTCAAGAACA TGTCGATCTC TGAAGACCTG 
GGGAAGTCTA CTGATGATGA TGATGATGAT GATGATGATG ATGAAAGTGG ATCCCACCCA 
AAGCTGCTCT GTGGTTCAGC CTTCGGTGGA TTCCCTGGGA CAGCGGTGTT TGATAGCGTT 
CAGTCGCTGG CGCAGAAGCT CTGCAGCTTA CAGGCCGTCA TCGCCCAGCA GGACAGCAGG 
ATCGAACTGC AGAATCTCTT CTGGTCAAAG AGCAAGCTGC CCGTCCGCAG CAACGCCAAC 
GCGCTGCTTG AGAAGGAGAA AGAGCGCAAC TTGGAGAAGC AGAGAGCAGA GCTGGCTAAG 
CTGCACAAGC AGCAGAACCA GCATCGAGCG GAGCAGCAGC GCTGGGAGAA AGAGAGACAG 
AAGCAGCAGT TGCTGATGGA AAGTCAGAAC GCCAAGATGC AGCAGCGGGA GGAGGAGTGC 
AAGATGATGG AGGAGAAACT AAAAGAAAAA GAGCTGAACC TGAAGCAGAG TCAGGAGACG 
TACCAACAGG ACCTGGAGAA TCTGAGGGAG ACCACACAGA AACTGGACAG GGAGTGGAAG 
ATGATTGAAC GGGAAAAGGA ACGTTTGGAA AAGATGAAGG CAAAGCTTGT CAACCATTAT 
GACGATCCGA CATGGTCTCC GGCGCTCTCC ACTGCCCCAT CATTCAGGAG CAGCATAGTA 
AACGGAGCAG GGACTCTGAC CCCAGTTCCC AATCCACTGG TCTTTACTAG TAACTCTCCT 
GACCCGGCAG AATCTCCTCC GCGGGTTCCT CCCCGGAGCA CCAGCATCAG AATGAGCAGG 
CAGATGCTGC CGGTTCAGTT GATGAGCACC ACCAACCAGG TGCAGACCCC TGTGGAGGTG 
CAGCAGAAGA TTCCCAGGAA GCTGGCGGCC GAACCCAAAA AGAAGGAGAA AACCAAGTCA 
AAGCGCTCCC ACAAGAGAGC CCACAGTGCA GCCAATATAG ATGTAACCCA GGTGATCCCG 
ATCCGGGTGA TGGGGAAAGA CAGTGGAAGT CTTAAAGCCC AAATGAGCAC AAGTCCAAAA 
AGAGTCTTAA GTGCAGACAC CTTCAGGACA CCAGGATCTG GTGACCTGAA GCCCAGATCC 
TGGTCACCAC CTTCAGGATC TGGTCGGCTT CCCAGAACGG CTTCCCAGAA CGTGAAGCCG 
TCTCAGTCGC TCATCGCACC GAAGTGGAAC AACGTTGGTT CTCTTCCACC GCGGCCTCCT 
CCCTTCCCGA CAGACATAAA TACGACAGAA CAAGAAAAGG TTATCGTTGT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103146077 ( XM_016677253.1 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_016532739.1 Poecilia formosa rho guanine nucleotide exchange factor 18-like (LOC103146077), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo146468
Clone ID Related Accession (Same CDS sequence)	XM_016677253.1
Accession Version	XM_016677253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	rho guanine nucleotide exchange factor 18-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800090.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGA CTGAAGGCCA GCGGGTGAAG CCTTTCGCTG ATGAATCTGC GTTATTGGCT 
GAGCCAATCA ACCCAGAAGA CTCCCATTAT GCTGAGCTGC GAGCTGATCT GGAGAGCGAC 
TCCCGCGGTC TGGAGGCGGA GTCCTGGAGC TCGGCTGTGG ACCAGAACTA CCTGATGTCT 
CTGAACAAAG AGGCTGTCAA AAGGCAGGAC GTGATCTACG AGTTGATCCA GACTGAGATG 
AACCATGTGC GAACCTTGAA AATCCTCCTC CACATCTACA TGTACGAGCT GAAGAAGTTC 
CAATCCATCA TCGACCTGGA GAAGCTCTTC CCCAGCCTGG AGGTTCTGCT CAGCCTCCAC 
TATGTCTTCC TCAAGTCCCT CAAATCCCGG AGAGATGAGA GTCGGGATGA GGACAATCCA 
AACAACTACT ACATCACAGA GCTGGCGGAT GTCCTCATCT ATCAGTTTTC AGGTAATCTG 
GGAATACAGA TGGCGACGGG TTACGGCGAC TTTTGCAGCC ATCACAGCGA AGCTGTCAGC 
TACTACAAGG AGCAAATGCA GAGCAACAAG ACGCTGCAAA TTTTGATTAG AAAAATCGAT 
CAGCTGCCTC TGGCGCGAAG ATTAACGATT CCTGAATGTT TCTTGCTGGT GACTCAACGC 
ATCACAAAAT ATCCGGTTCT GTTGGAGCGA ATCATCCAGA ACACAGACGC TGACACACAT 
GAGCACAGGC AGCTGGAGAA AGCTCTGGAG CTGATCAAGC ACACCATCTC CCAGGTGAAC 
ACCCAGGTGA GTGAATTTGG GAAGACCGCT CGCCTCAGAG AGATCATTAA TCGACTGGAG 
CAAAAGACTC AGGCCAAGCT GAAAGATGGC CGCGGGTTCT GGAGAGACGA CCTGAGGCAC 
GGCAACAGGA CGCTGGTGCA TGAAGGACCC CTGACCTGGA AGTTGTCCGG TAAACAAAAA 
GAAATTCATG CTGTGCTGCT GTCAGATATG CTGCTTCTGC TACAGGAAAA AGATCAGAAG 
CTTGTATTAG CTGCCATGGA TAGCAAACCG CCAGTCATCT ACCTGCAGGG AGTGATTGTG 
AGGGTAGTGG CCATGGAGGA AAGAGCCATG TACGTCATCT GTGCGTGTCC CTCCGGCTCG 
CCAGAAATGT ACGAGCTCCA CACGAGCTCC AAAGAGGAGT GCAAAACCTG GATGGACCTC 
ATACACAACA TGGCAGAAAG TTGCAGAGAC AATGAAGTGC ACAAAGAAAT GGTTACTAAA 
CTGCAGCAGT ATCAAGATAA GTTGCAGAAA AGAGACGAGC AGATAAAGAA AAGTCTGGAA 
AAGAAACAGG AAATTTTTGC TGAACTTTAT CATGACGTGT TGGGTCAGAA AAGCCTTTAC 
AACGGGCTGC TGCTGCGAGG CGATGCTTCT GACCTCCAGG AGGGGGAGAC CTTGTTGAAT 
GGGGCCATCG AAGAGGTGGA AAAGTTACAG ACCCTGCTCT TTATGATGAT TAAACATCCA 
GCCACTCCTG AGGGGGAAAT TGAGACACAG CAAAGTGTCA GGAGGGCACA GACCTTCAAA 
GGCTCTCTGG ATAATCTGAT AGCTAAAAGC ATGAAATACG AAGATGCAGC TGAGAAGGTG 
CAACGCAGAT ACAGCAGCTG TGACCTTCTG CTCAAGAACA TGTCGATCTC TGAAGACCTG 
GGGAAGTCTA CTGATGATGA TGATGATGAT GATGATGATG ATGAAAGTGG ATCCCACCCA 
AAGCTGCTCT GTGGTTCAGC CTTCGGTGGA TTCCCTGGGA CAGCGGTGTT TGATAGCGTT 
CAGTCGCTGG CGCAGAAGCT CTGCAGCTTA CAGGCCGTCA TCGCCCAGCA GGACAGCAGG 
ATCGAACTGC AGAATCTCTT CTGGTCAAAG AGCAAGCTGC CCGTCCGCAG CAACGCCAAC 
GCGCTGCTTG AGAAGGAGAA AGAGCGCAAC TTGGAGAAGC AGAGAGCAGA GCTGGCTAAG 
CTGCACAAGC AGCAGAACCA GCATCGAGCG GAGCAGCAGC GCTGGGAGAA AGAGAGACAG 
AAGCAGCAGT TGCTGATGGA AAGTCAGAAC GCCAAGATGC AGCAGCGGGA GGAGGAGTGC 
AAGATGATGG AGGAGAAACT AAAAGAAAAA GAGCTGAACC TGAAGCAGAG TCAGGAGACG 
TACCAACAGG ACCTGGAGAA TCTGAGGGAG ACCACACAGA AACTGGACAG GGAGTGGAAG 
ATGATTGAAC GGGAAAAGGA ACGTTTGGAA AAGATGAAGG CAAAGCTTGT CAACCATTAT 
GACGATCCGA CATGGTCTCC GGCGCTCTCC ACTGCCCCAT CATTCAGGAG CAGCATAGTA 
AACGGAGCAG GGACTCTGAC CCCAGTTCCC AATCCACTGG TCTTTACTAG TAACTCTCCT 
GACCCGGCAG AATCTCCTCC GCGGGTTCCT CCCCGGAGCA CCAGCATCAG AATGAGCAGG 
CAGATGCTGC CGGTTCAGTT GATGAGCACC ACCAACCAGG TGCAGACCCC TGTGGAGGTG 
CAGCAGAAGA TTCCCAGGAA GCTGGCGGCC GAACCCAAAA AGAAGGAGAA AACCAAGTCA 
AAGCGCTCCC ACAAGAGAGC CCACAGTGCA GCCAATATAG ATGTAACCCA GGTGATCCCG 
ATCCGGGTGA TGGGGAAAGA CAGTGGAAGT CTTAAAGCCC AAATGAGCAC AAGTCCAAAA 
AGAGTCTTAA GTGCAGACAC CTTCAGGACA CCAGGATCTG CCCAGAACGT GAAGCCGTCT 
CAGTCGCTCA TCGCACCGAA GTGGAACAAC GTTGGTTCTC TTCCACCGCG GCCTCCTCCC 
TTCCCGACAG ACATAAATAC GACAGAACAA GAAAAGGTTA TCGTTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016532739.1
CDS	524..3394
Translation	MDETEGQRVKPFADESALLAEPINPEDSHYAELRADLESDSRGLEAESWSSAVDQNYLMSLNKEAVKRQDVIYELIQTEMNHVRTLKILLHIYMYELKKFQSIIDLEKLFPSLEVLLSLHYVFLKSLKSRRDESRDEDNPNNYYITELADVLIYQFSGNLGIQMATGYGDFCSHHSEAVSYYKEQMQSNKTLQILIRKIDQLPLARRLTIPECFLLVTQRITKYPVLLERIIQNTDADTHEHRQLEKALELIKHTISQVNTQVSEFGKTARLREIINRLEQKTQAKLKDGRGFWRDDLRHGNRTLVHEGPLTWKLSGKQKEIHAVLLSDMLLLLQEKDQKLVLAAMDSKPPVIYLQGVIVRVVAMEERAMYVICACPSGSPEMYELHTSSKEECKTWMDLIHNMAESCRDNEVHKEMVTKLQQYQDKLQKRDEQIKKSLEKKQEIFAELYHDVLGQKSLYNGLLLRGDASDLQEGETLLNGAIEEVEKLQTLLFMMIKHPATPEGEIETQQSVRRAQTFKGSLDNLIAKSMKYEDAAEKVQRRYSSCDLLLKNMSISEDLGKSTDDDDDDDDDDESGSHPKLLCGSAFGGFPGTAVFDSVQSLAQKLCSLQAVIAQQDSRIELQNLFWSKSKLPVRSNANALLEKEKERNLEKQRAELAKLHKQQNQHRAEQQRWEKERQKQQLLMESQNAKMQQREEECKMMEEKLKEKELNLKQSQETYQQDLENLRETTQKLDREWKMIEREKERLEKMKAKLVNHYDDPTWSPALSTAPSFRSSIVNGAGTLTPVPNPLVFTSNSPDPAESPPRVPPRSTSIRMSRQMLPVQLMSTTNQVQTPVEVQQKIPRKLAAEPKKKEKTKSKRSHKRAHSAANIDVTQVIPIRVMGKDSGSLKAQMSTSPKRVLSADTFRTPGSAQNVKPSQSLIAPKWNNVGSLPPRPPPFPTDINTTEQEKVIVV

Target ORF information:

RefSeq Version	XM_016677253.1
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa rho guanine nucleotide exchange factor 18-like (LOC103146077), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016677253.1

ORF Insert Sequence:

ATGGATGAGA CTGAAGGCCA GCGGGTGAAG CCTTTCGCTG ATGAATCTGC GTTATTGGCT 
GAGCCAATCA ACCCAGAAGA CTCCCATTAT GCTGAGCTGC GAGCTGATCT GGAGAGCGAC 
TCCCGCGGTC TGGAGGCGGA GTCCTGGAGC TCGGCTGTGG ACCAGAACTA CCTGATGTCT 
CTGAACAAAG AGGCTGTCAA AAGGCAGGAC GTGATCTACG AGTTGATCCA GACTGAGATG 
AACCATGTGC GAACCTTGAA AATCCTCCTC CACATCTACA TGTACGAGCT GAAGAAGTTC 
CAATCCATCA TCGACCTGGA GAAGCTCTTC CCCAGCCTGG AGGTTCTGCT CAGCCTCCAC 
TATGTCTTCC TCAAGTCCCT CAAATCCCGG AGAGATGAGA GTCGGGATGA GGACAATCCA 
AACAACTACT ACATCACAGA GCTGGCGGAT GTCCTCATCT ATCAGTTTTC AGGTAATCTG 
GGAATACAGA TGGCGACGGG TTACGGCGAC TTTTGCAGCC ATCACAGCGA AGCTGTCAGC 
TACTACAAGG AGCAAATGCA GAGCAACAAG ACGCTGCAAA TTTTGATTAG AAAAATCGAT 
CAGCTGCCTC TGGCGCGAAG ATTAACGATT CCTGAATGTT TCTTGCTGGT GACTCAACGC 
ATCACAAAAT ATCCGGTTCT GTTGGAGCGA ATCATCCAGA ACACAGACGC TGACACACAT 
GAGCACAGGC AGCTGGAGAA AGCTCTGGAG CTGATCAAGC ACACCATCTC CCAGGTGAAC 
ACCCAGGTGA GTGAATTTGG GAAGACCGCT CGCCTCAGAG AGATCATTAA TCGACTGGAG 
CAAAAGACTC AGGCCAAGCT GAAAGATGGC CGCGGGTTCT GGAGAGACGA CCTGAGGCAC 
GGCAACAGGA CGCTGGTGCA TGAAGGACCC CTGACCTGGA AGTTGTCCGG TAAACAAAAA 
GAAATTCATG CTGTGCTGCT GTCAGATATG CTGCTTCTGC TACAGGAAAA AGATCAGAAG 
CTTGTATTAG CTGCCATGGA TAGCAAACCG CCAGTCATCT ACCTGCAGGG AGTGATTGTG 
AGGGTAGTGG CCATGGAGGA AAGAGCCATG TACGTCATCT GTGCGTGTCC CTCCGGCTCG 
CCAGAAATGT ACGAGCTCCA CACGAGCTCC AAAGAGGAGT GCAAAACCTG GATGGACCTC 
ATACACAACA TGGCAGAAAG TTGCAGAGAC AATGAAGTGC ACAAAGAAAT GGTTACTAAA 
CTGCAGCAGT ATCAAGATAA GTTGCAGAAA AGAGACGAGC AGATAAAGAA AAGTCTGGAA 
AAGAAACAGG AAATTTTTGC TGAACTTTAT CATGACGTGT TGGGTCAGAA AAGCCTTTAC 
AACGGGCTGC TGCTGCGAGG CGATGCTTCT GACCTCCAGG AGGGGGAGAC CTTGTTGAAT 
GGGGCCATCG AAGAGGTGGA AAAGTTACAG ACCCTGCTCT TTATGATGAT TAAACATCCA 
GCCACTCCTG AGGGGGAAAT TGAGACACAG CAAAGTGTCA GGAGGGCACA GACCTTCAAA 
GGCTCTCTGG ATAATCTGAT AGCTAAAAGC ATGAAATACG AAGATGCAGC TGAGAAGGTG 
CAACGCAGAT ACAGCAGCTG TGACCTTCTG CTCAAGAACA TGTCGATCTC TGAAGACCTG 
GGGAAGTCTA CTGATGATGA TGATGATGAT GATGATGATG ATGAAAGTGG ATCCCACCCA 
AAGCTGCTCT GTGGTTCAGC CTTCGGTGGA TTCCCTGGGA CAGCGGTGTT TGATAGCGTT 
CAGTCGCTGG CGCAGAAGCT CTGCAGCTTA CAGGCCGTCA TCGCCCAGCA GGACAGCAGG 
ATCGAACTGC AGAATCTCTT CTGGTCAAAG AGCAAGCTGC CCGTCCGCAG CAACGCCAAC 
GCGCTGCTTG AGAAGGAGAA AGAGCGCAAC TTGGAGAAGC AGAGAGCAGA GCTGGCTAAG 
CTGCACAAGC AGCAGAACCA GCATCGAGCG GAGCAGCAGC GCTGGGAGAA AGAGAGACAG 
AAGCAGCAGT TGCTGATGGA AAGTCAGAAC GCCAAGATGC AGCAGCGGGA GGAGGAGTGC 
AAGATGATGG AGGAGAAACT AAAAGAAAAA GAGCTGAACC TGAAGCAGAG TCAGGAGACG 
TACCAACAGG ACCTGGAGAA TCTGAGGGAG ACCACACAGA AACTGGACAG GGAGTGGAAG 
ATGATTGAAC GGGAAAAGGA ACGTTTGGAA AAGATGAAGG CAAAGCTTGT CAACCATTAT 
GACGATCCGA CATGGTCTCC GGCGCTCTCC ACTGCCCCAT CATTCAGGAG CAGCATAGTA 
AACGGAGCAG GGACTCTGAC CCCAGTTCCC AATCCACTGG TCTTTACTAG TAACTCTCCT 
GACCCGGCAG AATCTCCTCC GCGGGTTCCT CCCCGGAGCA CCAGCATCAG AATGAGCAGG 
CAGATGCTGC CGGTTCAGTT GATGAGCACC ACCAACCAGG TGCAGACCCC TGTGGAGGTG 
CAGCAGAAGA TTCCCAGGAA GCTGGCGGCC GAACCCAAAA AGAAGGAGAA AACCAAGTCA 
AAGCGCTCCC ACAAGAGAGC CCACAGTGCA GCCAATATAG ATGTAACCCA GGTGATCCCG 
ATCCGGGTGA TGGGGAAAGA CAGTGGAAGT CTTAAAGCCC AAATGAGCAC AAGTCCAAAA 
AGAGTCTTAA GTGCAGACAC CTTCAGGACA CCAGGATCTG CCCAGAACGT GAAGCCGTCT 
CAGTCGCTCA TCGCACCGAA GTGGAACAAC GTTGGTTCTC TTCCACCGCG GCCTCCTCCC 
TTCCCGACAG ACATAAATAC GACAGAACAA GAAAAGGTTA TCGTTGTTTA A