gck cDNA ORF clone, Poecilia formosa(Amazon molly)

Gene
Clones
gRNAs

Summary

Gene Symbol	gck
Entrez Gene ID	103145883
Full Name	glucokinase
Gene Type	protein-coding
Organism	Poecilia formosa(Amazon molly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007563533.2	XP_007563595.1	glucokinase

Pathways from BioSystems
Gene Ontology

The following gck gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gck cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo146293	XM_007563533.2 Latest version!	Poecilia formosa glucokinase (gck), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gck ( XM_007563533.2 ) cDNA ORF clone, Poecilia formosa(Amazon molly) -> XP_007563595.1 Poecilia formosa glucokinase (gck), mRNA.

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CloneID	OPo146293
Clone ID Related Accession (Same CDS sequence)	XM_007563533.2
Accession Version	XM_007563533.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Poecilia formosa(Amazon molly)
Product	glucokinase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006800087.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 3, 2016 this sequence version replaced XM_007563533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia formosa Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTGCA TCACCAGCCA ACTCGACCAG ATGATGAAGC TCCCTGGTAG CTACAGTGAC 
GTGGTTGAAA AAATATGCAT GGTGGAGCAG ATCCTGTCTG AGTTTAGATT GAACAAAGAG 
GAGCTGGAAG AAGTCATGAA GAGGATGCAG CATGAAATGA ACAGAGGACT GCGTTTAGAG 
ACCCACGAAG AGGCCAGCGT CAAAATGCTT CCGACCTACG TCTGCTCCAC CCCCGAGGGA 
TCAGAAGTGG GAGATTTCTT GGCTCTGGAC CTCGGAGGGA CAAATTTCCG TGTGATGCTT 
GTGAAAGTGG GTGAAGACGA AGAGAGAAGT TTCAAGGTGG AGACGAAGAA CCAAATGTAT 
TCCATCCCTG AAGATGCCAT GACGGGGACT GCTGAAATGC TGTTTGACTA CATAGCAGAA 
TGCATGTCTG ACTTTTTGGA CAAACACCAC ATCAAGCACA AGAAGCTTCC TCTGGGTTTC 
ACCTTCTCCT TCCCTGTACG ACATGAAGAC CTTGATAAGG GAATCTTGCT TAACTGGACC 
AAAGGTTTCA AGGCCTCTGG GGCTGAAGGG AACAATGTTG TTGGTTTGCT CAGAGATGCA 
ATCAAGAGAC GAGGGGACTT TGAGATGGAT GTGGTTGCCA TGGTGAATGA CACAGTTGCA 
ACCATGATCT CCTGCTACTA CGAGGACAGA AGCTGTGAAG TCGGGATGAT TGTCGGTACT 
GGCTGTAATG CCTGTTACAT GGAGGAGATG AGGACTGTGG AGCTGGTGGA GGGGGAGGAG 
GGCCGGATGT GTGTGAACAC TGAGTGGGGA GCATTTGGAG ACTCGGGCGA GCTGGAGGAG 
TTCAGGCTGG AGTACGACAG GGTGGTGGAC GAGACCTCAA TCAACCCTGG ACACCAGCTG 
TATGAGAAGA TTATCAGTGG AAAGTACATG GGGGAACTGG CGAGACTCGT CATGATGAAG 
CTGGTGAATG AGAACCTGCT GTTTAACGGC GAAGCCTCAG AGCAGCTCAA GACACGAGGC 
GGCTTCGAGA CGCGCTTCGT CTCCCAGGTT GAGAGTGACT CTGGCGACAG AAAGCAGATC 
TACAACATCC TCTCCTCTCT GGGTGTCCTT CCCTCTGAGC TGGACTGCGA CATTGTGCGT 
CTGGTCTGCG AGAGCGTCTC CACTCGCTCT GCTCATATGT GTGGCGCAGG CCTCGCTGGG 
GTCATTAACC AGATGCAAGA GCGACGCGGC CAAGATACCA TGAAGATCAC AGTCGGGGTT 
GACGGATCCG TCTACAAGCT GCACCCTTAT TTCCGTGACC GGTTCCACCA GGTCGTCAGG 
GAACTCACCC CTCATTCTGA GATAACCTTC CTACAGTCGG AGGAGGGCAG CGGGCGTGGA 
GCTGCTCTGA TCTCTGCAGT GGCTTGTAAG ATGGCATCAT GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007563595.1
CDS	119..1546
Translation	MPCITSQLDQMMKLPGSYSDVVEKICMVEQILSEFRLNKEELEEVMKRMQHEMNRGLRLETHEEASVKMLPTYVCSTPEGSEVGDFLALDLGGTNFRVMLVKVGEDEERSFKVETKNQMYSIPEDAMTGTAEMLFDYIAECMSDFLDKHHIKHKKLPLGFTFSFPVRHEDLDKGILLNWTKGFKASGAEGNNVVGLLRDAIKRRGDFEMDVVAMVNDTVATMISCYYEDRSCEVGMIVGTGCNACYMEEMRTVELVEGEEGRMCVNTEWGAFGDSGELEEFRLEYDRVVDETSINPGHQLYEKIISGKYMGELARLVMMKLVNENLLFNGEASEQLKTRGGFETRFVSQVESDSGDRKQIYNILSSLGVLPSELDCDIVRLVCESVSTRSAHMCGAGLAGVINQMQERRGQDTMKITVGVDGSVYKLHPYFRDRFHQVVRELTPHSEITFLQSEEGSGRGAALISAVACKMASWV

Target ORF information:

RefSeq Version	XM_007563533.2
Organism	Poecilia formosa(Amazon molly)
Definition	Poecilia formosa glucokinase (gck), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007563533.2

ORF Insert Sequence:

ATGCCGTGCA TCACCAGCCA ACTCGACCAG ATGATGAAGC TCCCTGGTAG CTACAGTGAC 
GTGGTTGAAA AAATATGCAT GGTGGAGCAG ATCCTGTCTG AGTTTAGATT GAACAAAGAG 
GAGCTGGAAG AAGTCATGAA GAGGATGCAG CATGAAATGA ACAGAGGACT GCGTTTAGAG 
ACCCACGAAG AGGCCAGCGT CAAAATGCTT CCGACCTACG TCTGCTCCAC CCCCGAGGGA 
TCAGAAGTGG GAGATTTCTT GGCTCTGGAC CTCGGAGGGA CAAATTTCCG TGTGATGCTT 
GTGAAAGTGG GTGAAGACGA AGAGAGAAGT TTCAAGGTGG AGACGAAGAA CCAAATGTAT 
TCCATCCCTG AAGATGCCAT GACGGGGACT GCTGAAATGC TGTTTGACTA CATAGCAGAA 
TGCATGTCTG ACTTTTTGGA CAAACACCAC ATCAAGCACA AGAAGCTTCC TCTGGGTTTC 
ACCTTCTCCT TCCCTGTACG ACATGAAGAC CTTGATAAGG GAATCTTGCT TAACTGGACC 
AAAGGTTTCA AGGCCTCTGG GGCTGAAGGG AACAATGTTG TTGGTTTGCT CAGAGATGCA 
ATCAAGAGAC GAGGGGACTT TGAGATGGAT GTGGTTGCCA TGGTGAATGA CACAGTTGCA 
ACCATGATCT CCTGCTACTA CGAGGACAGA AGCTGTGAAG TCGGGATGAT TGTCGGTACT 
GGCTGTAATG CCTGTTACAT GGAGGAGATG AGGACTGTGG AGCTGGTGGA GGGGGAGGAG 
GGCCGGATGT GTGTGAACAC TGAGTGGGGA GCATTTGGAG ACTCGGGCGA GCTGGAGGAG 
TTCAGGCTGG AGTACGACAG GGTGGTGGAC GAGACCTCAA TCAACCCTGG ACACCAGCTG 
TATGAGAAGA TTATCAGTGG AAAGTACATG GGGGAACTGG CGAGACTCGT CATGATGAAG 
CTGGTGAATG AGAACCTGCT GTTTAACGGC GAAGCCTCAG AGCAGCTCAA GACACGAGGC 
GGCTTCGAGA CGCGCTTCGT CTCCCAGGTT GAGAGTGACT CTGGCGACAG AAAGCAGATC 
TACAACATCC TCTCCTCTCT GGGTGTCCTT CCCTCTGAGC TGGACTGCGA CATTGTGCGT 
CTGGTCTGCG AGAGCGTCTC CACTCGCTCT GCTCATATGT GTGGCGCAGG CCTCGCTGGG 
GTCATTAACC AGATGCAAGA GCGACGCGGC CAAGATACCA TGAAGATCAC AGTCGGGGTT 
GACGGATCCG TCTACAAGCT GCACCCTTAT TTCCGTGACC GGTTCCACCA GGTCGTCAGG 
GAACTCACCC CTCATTCTGA GATAACCTTC CTACAGTCGG AGGAGGGCAG CGGGCGTGGA 
GCTGCTCTGA TCTCTGCAGT GGCTTGTAAG ATGGCATCAT GGGTCTGA