Bub1b cDNA ORF clone, Peromyscus maniculatus bairdii(prairie deer mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bub1b
Entrez Gene ID	102921947
Full Name	BUB1 mitotic checkpoint serine/threonine kinase B
Gene Type	protein-coding
Organism	Peromyscus maniculatus bairdii(prairie deer mouse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006979228.2	XP_006979290.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X2
XM_015997903.1	XP_015853389.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1

Pathways from BioSystems
Gene Ontology

The following Bub1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bub1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPe65294	XM_015997903.1 Latest version!	Peromyscus maniculatus bairdii BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPe65295	XM_006979228.2 Latest version!	Peromyscus maniculatus bairdii BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bub1b ( XM_015997903.1 ) cDNA ORF clone, Peromyscus maniculatus bairdii(prairie deer mouse) -> XP_015853389.1 Peromyscus maniculatus bairdii BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPe65294
Clone ID Related Accession (Same CDS sequence)	XM_015997903.1
Accession Version	XM_015997903.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Peromyscus maniculatus bairdii(prairie deer mouse)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006501151.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Peromyscus maniculatus bairdii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTGT CTAGGAGCAC GGGGAGACGG TCTCCGCAAC TCCAGGGCCA GCTGCAGGTT 
GAGGAAGGAA GCTCGGACAG TCGGCACCCG GAGGAGAAAG GTCTGCGGAC GGGCGAGAGC 
AAGAATCGGG AATGCGGGAT GGCGGCGGCG AGGAAGGAAG GGCTTGCAGC GAGTGAAGCC 
ATGTCCCTGG AGGGAGATGA GTGGGAGCTG AGTAAAGAGA ACGTGCAACC CTTACGGCAT 
GGCCGGATCA TGTCCACGCT CCAGGGAGCT CTGGCACAGC AGGAATCAGC CTGCAACACT 
ACCCTTCAGC AACAGAAGCG GGCATTTGAA TCTGAAATCC GCTTTTACTC TGGAAATGAC 
CCTCTGGATG TTTGGGACAG GTATATTACC TGGACCGAGC AGAGCTACCC TCAAGGCGGG 
AAAGAGAGTA ACATGTCGAC GTTACTAGAA AGAGCCGTAG AAGCTCTACA AGGAGAGACG 
CGCTACTACA GTGACCCCCG GTTTCTCAGT CTCTGGCTCA AATTGGGGCA CCTGTGCAAC 
GAGCCCTTGG ATTTGTACAG CTATTTACAC AGCCAGGGGA TTGGCGTCTC TCTTGCCCAG 
TTCTACATCT CATGGGCAGA AGAATACGAA GCTAGAGAAA ACTTCAAGAG AGCGGACGCC 
GTGTTCCAGG AAGGGATTGA ACGCAAGGCT GAGCCCCTGG AAAGACTGCA GTCCCAGCAC 
AGACAATTCC AGGCTCGAGT GTCTCGACAA GCTCTCTTGG CACTTGAGAA GGAGGAGGAG 
GAGGAGGTCC TGGGCTCTTC TGTACCACAG AGAAGCACGC TTGCTGAGCT GAAGAGCCGA 
GGGAAGAAGA CAGCCAAGGC GCCCATCAGC CGTGTGGGAG GCGCTCTGAA GGCTCCGGGT 
CAGAGCAGAG GATTCCAAAA TATAGTTCCT CAGCAGATGT GCAGTAACCG CAGGATCACT 
GTTTTTGATG AAAACGCTGA TAACTCTTCG AAGGCAGAGT TACCTAAGCC TGTCGTCCAA 
CCATGGATGG CGCCCCCTAT CTCTAAGGCC AAAGAGAATG AGCTACAGCC AGGCCCGTGG 
AACGCAGACA GACCCTTGGA TTATAGGCCT CATGGCAGTT CGGCCTCCGT AGTAGCTGCG 
CCCCCTTCGC TTCCCAGCTT TACGCCCTAT GTGGAAGAGA GCGCTCAGCA GACAGTCATG 
ACACCATGTA AAATTGAACC TAGTATCAAC CATGTTCTCA GCACCAGAAA GCCTGGGAAG 
GAAGAAGGAG ACCCCTTGCA AAGGGTTCAG AGTCATCAGC AAGGGTCTGA GGAGAGGAAG 
GAGAAGATGA TGTACTGTAA GGACAAGATT TATGCTGGAG TCGGGGAGTT CTCCTTCGAG 
GAGATCCGAG CTGAAGTGTT CCGGAAGAAA CTGAAGGAGC GAAGGGAAGC TGAACTGCTG 
ACCAGTGCAG AAAAGCGGGC AGAAATGCAG AAACAGATTG AGGAGATGGA GAGAAAGCTG 
AAGGAAATCC AGACTATTCA ACAAGAAAGA GCTGGTGACC AGCAGCAAGA AGAGAAGGTG 
CCTACAGAGG AGACCACCAG ACTGCAGATT CCTTCGGAGT CCCAGGAAAG GCTGGCAACA 
TCTCTGTCCC GTTCTGTCTG TCCACCAAGC TCTCACCCCA GGGAAACTTC ACCAGCTAAA 
AACATTTTGC AAGAACAGCC TGACTCTAAA GGTCCCAGCA TGCCTTTCTC CATTTTCGAT 
GAGTTTCTTT CGGACGACAA GGACAAAAGT CTGGCTACAG ATCTTCCACA GGTTCCCAAA 
GCCCAGCGAA GACCCCTTGC TGTTCTCAAA ACCACGGAAA TCATTGCCTC AAATGAGGAG 
ATGTCTCCTG ATGTTTGTGA TGAACTTACA GGAATTGAAC CTTTGAGTGA AGACGCCATC 
ATCACAGGTT TCAGTAACGC GACCATCTGT CCCAACCCCG AGGACACTTG TGACTTTGTT 
AGAGCAGCTC GTTTTGCGTC TACTCCTTTC CCTGAGATAC TGTCCTTGAA GGGGGTCCCT 
TCCGGTCCTG AGAGACTGTT GCAGGAAGAG GATCTTGATG GCAAGACCAC CGAGGGCTGT 
CTCACCGCAC GGACCACTCT CTATAACCAG ACCCTCAGCG TCAAGAAGCT GAGCCCGATT 
ATTGAAGACA GTCGGGAAGC CACACACTCC TCCGGCTTCT CTGGATCCTC CGCTTCAGTT 
ACGAGTACCT CCTCCATCAA AGGCCTTCAG CTTCCCGAGA AGCTGGAGCT GACTAATGAC 
ACCACAGAAA ATGCTATTCA GTCACCCTGG TGCTCACAAT ATCGGCTACA ACTACTGAAG 
TCCCTACCAG AGTCAAGTGA TTCTGCAGAG TTTTCTCTGG AAGATAGACC AATGCCGACC 
CTGGAGATAG GGGAGGAGAT TGAGTTAGGT GACGAAGACT ACTGCATTAA ACAGGAATAC 
CTAGTATGTG AAGAGTACAA GTTATTCTGG GCGAAATCTA GAAACTCTGC AGAGTTAACC 
ATGATAAAGG CATCTTTTCA ACCTATCCCA TGGGATTTTT ATGTCAACCT CAAGTTAAAG 
GAACGTCTAA ACGAGGACTC TGATCAGCTG TGCAGCTACT ATCAGTACCA AGACGGCCAT 
ATCGTTTCCT ACCAATATGT CAAGTGTTGC ACCCTTCAGG ATCTTCTCCA GCACAGTGAG 
TCTGTGTCTC ATGAAGTAAC CGTCTTGATT ATTTATAACC TCTTGACAAT AGTGGAGAAA 
CTCCACAAAG CTGAAATAGT TCATGGAGAC CTGAGTCCAA GGAGTCTGAT CCTAAGAAAC 
AGAATCCATG ACCCCTATGA TTGCAATAAG AAGGATCATG CTGTGAAGAT CGTGGACTTC 
TCCTACAGTG TTGACCTGAG GGTGCAGCTA GATGCTTTTT CCTTCAGTGG CTTTCGGACT 
GTACAGACCC TGGAAGGACA AAAGATCCTG GAGAACTGTT CTTCACCCTA CCAGGTAGAT 
CTGTTGGGCA TAGCAGACCT AGCACACTTA CTGTTGTTCA AGGAACACCT GCATGTCTTC 
TGGGATGGGC TTCTCTGGAG ACTCAGCCAG ACTACTTCTG AGCTAAAGAA TGGTGAATTA 
TGGAATAAAT TCTTTGTGCG GATTCTGAAT GCCAGCGATG AGCCCACAGT ATTGGTTCTG 
AGGGAACTTG CAGCGGAAGT GAACGGGGTT TTTGACACCA CATTCCACAG TCACCTGGAC 
AAAGCCTTGT GGAAGAAGGA AGTGAGGACT GCTGAGGGGC TAGAAGACGG TTTGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015853389.1
CDS	88..3387
Translation	MLLSRSTGRRSPQLQGQLQVEEGSSDSRHPEEKGLRTGESKNRECGMAAARKEGLAASEAMSLEGDEWELSKENVQPLRHGRIMSTLQGALAQQESACNTTLQQQKRAFESEIRFYSGNDPLDVWDRYITWTEQSYPQGGKESNMSTLLERAVEALQGETRYYSDPRFLSLWLKLGHLCNEPLDLYSYLHSQGIGVSLAQFYISWAEEYEARENFKRADAVFQEGIERKAEPLERLQSQHRQFQARVSRQALLALEKEEEEEVLGSSVPQRSTLAELKSRGKKTAKAPISRVGGALKAPGQSRGFQNIVPQQMCSNRRITVFDENADNSSKAELPKPVVQPWMAPPISKAKENELQPGPWNADRPLDYRPHGSSASVVAAPPSLPSFTPYVEESAQQTVMTPCKIEPSINHVLSTRKPGKEEGDPLQRVQSHQQGSEERKEKMMYCKDKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAEKRAEMQKQIEEMERKLKEIQTIQQERAGDQQQEEKVPTEETTRLQIPSESQERLATSLSRSVCPPSSHPRETSPAKNILQEQPDSKGPSMPFSIFDEFLSDDKDKSLATDLPQVPKAQRRPLAVLKTTEIIASNEEMSPDVCDELTGIEPLSEDAIITGFSNATICPNPEDTCDFVRAARFASTPFPEILSLKGVPSGPERLLQEEDLDGKTTEGCLTARTTLYNQTLSVKKLSPIIEDSREATHSSGFSGSSASVTSTSSIKGLQLPEKLELTNDTTENAIQSPWCSQYRLQLLKSLPESSDSAEFSLEDRPMPTLEIGEEIELGDEDYCIKQEYLVCEEYKLFWAKSRNSAELTMIKASFQPIPWDFYVNLKLKERLNEDSDQLCSYYQYQDGHIVSYQYVKCCTLQDLLQHSESVSHEVTVLIIYNLLTIVEKLHKAEIVHGDLSPRSLILRNRIHDPYDCNKKDHAVKIVDFSYSVDLRVQLDAFSFSGFRTVQTLEGQKILENCSSPYQVDLLGIADLAHLLLFKEHLHVFWDGLLWRLSQTTSELKNGELWNKFFVRILNASDEPTVLVLRELAAEVNGVFDTTFHSHLDKALWKKEVRTAEGLEDGLA

Target ORF information:

RefSeq Version	XM_015997903.1
Organism	Peromyscus maniculatus bairdii(prairie deer mouse)
Definition	Peromyscus maniculatus bairdii BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015997903.1

ORF Insert Sequence:

ATGCTTTTGT CTAGGAGCAC GGGGAGACGG TCTCCGCAAC TCCAGGGCCA GCTGCAGGTT 
GAGGAAGGAA GCTCGGACAG TCGGCACCCG GAGGAGAAAG GTCTGCGGAC GGGCGAGAGC 
AAGAATCGGG AATGCGGGAT GGCGGCGGCG AGGAAGGAAG GGCTTGCAGC GAGTGAAGCC 
ATGTCCCTGG AGGGAGATGA GTGGGAGCTG AGTAAAGAGA ACGTGCAACC CTTACGGCAT 
GGCCGGATCA TGTCCACGCT CCAGGGAGCT CTGGCACAGC AGGAATCAGC CTGCAACACT 
ACCCTTCAGC AACAGAAGCG GGCATTTGAA TCTGAAATCC GCTTTTACTC TGGAAATGAC 
CCTCTGGATG TTTGGGACAG GTATATTACC TGGACCGAGC AGAGCTACCC TCAAGGCGGG 
AAAGAGAGTA ACATGTCGAC GTTACTAGAA AGAGCCGTAG AAGCTCTACA AGGAGAGACG 
CGCTACTACA GTGACCCCCG GTTTCTCAGT CTCTGGCTCA AATTGGGGCA CCTGTGCAAC 
GAGCCCTTGG ATTTGTACAG CTATTTACAC AGCCAGGGGA TTGGCGTCTC TCTTGCCCAG 
TTCTACATCT CATGGGCAGA AGAATACGAA GCTAGAGAAA ACTTCAAGAG AGCGGACGCC 
GTGTTCCAGG AAGGGATTGA ACGCAAGGCT GAGCCCCTGG AAAGACTGCA GTCCCAGCAC 
AGACAATTCC AGGCTCGAGT GTCTCGACAA GCTCTCTTGG CACTTGAGAA GGAGGAGGAG 
GAGGAGGTCC TGGGCTCTTC TGTACCACAG AGAAGCACGC TTGCTGAGCT GAAGAGCCGA 
GGGAAGAAGA CAGCCAAGGC GCCCATCAGC CGTGTGGGAG GCGCTCTGAA GGCTCCGGGT 
CAGAGCAGAG GATTCCAAAA TATAGTTCCT CAGCAGATGT GCAGTAACCG CAGGATCACT 
GTTTTTGATG AAAACGCTGA TAACTCTTCG AAGGCAGAGT TACCTAAGCC TGTCGTCCAA 
CCATGGATGG CGCCCCCTAT CTCTAAGGCC AAAGAGAATG AGCTACAGCC AGGCCCGTGG 
AACGCAGACA GACCCTTGGA TTATAGGCCT CATGGCAGTT CGGCCTCCGT AGTAGCTGCG 
CCCCCTTCGC TTCCCAGCTT TACGCCCTAT GTGGAAGAGA GCGCTCAGCA GACAGTCATG 
ACACCATGTA AAATTGAACC TAGTATCAAC CATGTTCTCA GCACCAGAAA GCCTGGGAAG 
GAAGAAGGAG ACCCCTTGCA AAGGGTTCAG AGTCATCAGC AAGGGTCTGA GGAGAGGAAG 
GAGAAGATGA TGTACTGTAA GGACAAGATT TATGCTGGAG TCGGGGAGTT CTCCTTCGAG 
GAGATCCGAG CTGAAGTGTT CCGGAAGAAA CTGAAGGAGC GAAGGGAAGC TGAACTGCTG 
ACCAGTGCAG AAAAGCGGGC AGAAATGCAG AAACAGATTG AGGAGATGGA GAGAAAGCTG 
AAGGAAATCC AGACTATTCA ACAAGAAAGA GCTGGTGACC AGCAGCAAGA AGAGAAGGTG 
CCTACAGAGG AGACCACCAG ACTGCAGATT CCTTCGGAGT CCCAGGAAAG GCTGGCAACA 
TCTCTGTCCC GTTCTGTCTG TCCACCAAGC TCTCACCCCA GGGAAACTTC ACCAGCTAAA 
AACATTTTGC AAGAACAGCC TGACTCTAAA GGTCCCAGCA TGCCTTTCTC CATTTTCGAT 
GAGTTTCTTT CGGACGACAA GGACAAAAGT CTGGCTACAG ATCTTCCACA GGTTCCCAAA 
GCCCAGCGAA GACCCCTTGC TGTTCTCAAA ACCACGGAAA TCATTGCCTC AAATGAGGAG 
ATGTCTCCTG ATGTTTGTGA TGAACTTACA GGAATTGAAC CTTTGAGTGA AGACGCCATC 
ATCACAGGTT TCAGTAACGC GACCATCTGT CCCAACCCCG AGGACACTTG TGACTTTGTT 
AGAGCAGCTC GTTTTGCGTC TACTCCTTTC CCTGAGATAC TGTCCTTGAA GGGGGTCCCT 
TCCGGTCCTG AGAGACTGTT GCAGGAAGAG GATCTTGATG GCAAGACCAC CGAGGGCTGT 
CTCACCGCAC GGACCACTCT CTATAACCAG ACCCTCAGCG TCAAGAAGCT GAGCCCGATT 
ATTGAAGACA GTCGGGAAGC CACACACTCC TCCGGCTTCT CTGGATCCTC CGCTTCAGTT 
ACGAGTACCT CCTCCATCAA AGGCCTTCAG CTTCCCGAGA AGCTGGAGCT GACTAATGAC 
ACCACAGAAA ATGCTATTCA GTCACCCTGG TGCTCACAAT ATCGGCTACA ACTACTGAAG 
TCCCTACCAG AGTCAAGTGA TTCTGCAGAG TTTTCTCTGG AAGATAGACC AATGCCGACC 
CTGGAGATAG GGGAGGAGAT TGAGTTAGGT GACGAAGACT ACTGCATTAA ACAGGAATAC 
CTAGTATGTG AAGAGTACAA GTTATTCTGG GCGAAATCTA GAAACTCTGC AGAGTTAACC 
ATGATAAAGG CATCTTTTCA ACCTATCCCA TGGGATTTTT ATGTCAACCT CAAGTTAAAG 
GAACGTCTAA ACGAGGACTC TGATCAGCTG TGCAGCTACT ATCAGTACCA AGACGGCCAT 
ATCGTTTCCT ACCAATATGT CAAGTGTTGC ACCCTTCAGG ATCTTCTCCA GCACAGTGAG 
TCTGTGTCTC ATGAAGTAAC CGTCTTGATT ATTTATAACC TCTTGACAAT AGTGGAGAAA 
CTCCACAAAG CTGAAATAGT TCATGGAGAC CTGAGTCCAA GGAGTCTGAT CCTAAGAAAC 
AGAATCCATG ACCCCTATGA TTGCAATAAG AAGGATCATG CTGTGAAGAT CGTGGACTTC 
TCCTACAGTG TTGACCTGAG GGTGCAGCTA GATGCTTTTT CCTTCAGTGG CTTTCGGACT 
GTACAGACCC TGGAAGGACA AAAGATCCTG GAGAACTGTT CTTCACCCTA CCAGGTAGAT 
CTGTTGGGCA TAGCAGACCT AGCACACTTA CTGTTGTTCA AGGAACACCT GCATGTCTTC 
TGGGATGGGC TTCTCTGGAG ACTCAGCCAG ACTACTTCTG AGCTAAAGAA TGGTGAATTA 
TGGAATAAAT TCTTTGTGCG GATTCTGAAT GCCAGCGATG AGCCCACAGT ATTGGTTCTG 
AGGGAACTTG CAGCGGAAGT GAACGGGGTT TTTGACACCA CATTCCACAG TCACCTGGAC 
AAAGCCTTGT GGAAGAAGGA AGTGAGGACT GCTGAGGGGC TAGAAGACGG TTTGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bub1b ( XM_006979228.2 ) cDNA ORF clone, Peromyscus maniculatus bairdii(prairie deer mouse) -> XP_006979290.1 Peromyscus maniculatus bairdii BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPe65295
Clone ID Related Accession (Same CDS sequence)	XM_006979228.2
Accession Version	XM_006979228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-03-20
Organism	Peromyscus maniculatus bairdii(prairie deer mouse)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006501151.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 21, 2016 this sequence version replaced XM_006979228.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Peromyscus maniculatus bairdii Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTGT CTAGGAGCAC GGGGAGACGG TCTCCGCAAC TCCAGGGCCA GCTGCAGGTT 
GAGGAAGGAA GCTCGGACAG TCGGCACCCG GAGGAGAAAG GTCTGCGGAC GGGCGAGAGC 
AAGAATCGGG AATGCGGGAT GGCGGCGGCG AGGAAGGAAG GGCTTGCAGC GAGTGAAGCC 
ATGTCCCTGG AGGGAGATGA GTGGGAGCTG AGTAAAGAGA ACGTGCAACC CTTACGGCAT 
GGCCGGATCA TGTCCACGCT CCAGGGAGCT CTGGCACAGC AGGAATCAGC CTGCAACACT 
ACCCTTCAGC AACAGAAGCG GGCATTTGAA TCTGAAATCC GCTTTTACTC TGGAAATGAC 
CCTCTGGATG TTTGGGACAG GTATATTACC TGGACCGAGC AGAGCTACCC TCAAGGCGGG 
AAAGAGAGTA ACATGTCGAC GTTACTAGAA AGAGCCGTAG AAGCTCTACA AGGAGAGACG 
CGCTACTACA GTGACCCCCG GTTTCTCAGT CTCTGGCTCA AATTGGGGCA CCTGTGCAAC 
GAGCCCTTGG ATTTGTACAG CTATTTACAC AGCCAGGGGA TTGGCGTCTC TCTTGCCCAG 
TTCTACATCT CATGGGCAGA AGAATACGAA GCTAGAGAAA ACTTCAAGAG AGCGGACGCC 
GTGTTCCAGG AAGGGATTGA ACGCAAGGCT GAGCCCCTGG AAAGACTGCA GTCCCAGCAC 
AGACAATTCC AGGCTCGAGT GTCTCGACAA GCTCTCTTGG CACTTGAGAA GGAGGAGGAG 
GAGGAGGTCC TGGGCTCTTC TGTACCACAG AGAAGCACGC TTGCTGAGCT GAAGAGCCGA 
GGGAAGAAGA CAGCCAAGGC GCCCATCAGC CGTGTGGGAG GCGCTCTGAA GGCTCCGGGT 
CAGAGCAGAG GATTCCAAAA TATAGTTCCT CAGCAGATGT GCAGTAACCG CAGGATCACT 
GTTTTTGATG AAAACGCTGA TAACTCTTCG AAGGCAGAGT TACCTAAGCC TGTCGTCCAA 
CCATGGATGG CGCCCCCTAT CTCTAAGGCC AAAGAGAATG AGCTACAGCC AGGCCCGTGG 
AACGCAGACA GACCCTTGGA TTATAGGCCT CATGGCAGTT CGGCCTCCGT AGTAGCTGCG 
CCCCCTTCGC TTCCCAGCTT TACGCCCTAT GTGGAAGAGA GCGCTCAGCA GACAGTCATG 
ACACCATGTA AAATTGAACC TAGTATCAAC CATGTTCTCA GCACCAGAAA GCCTGGGAAG 
GAAGAAGGAG ACCCCTTGCA AAGGGTTCAG AGTCATCAGC AAGGGTCTGA GGAGAGGAAG 
GAGAAGATGA TGTACTGTAA GGACAAGATT TATGCTGGAG TCGGGGAGTT CTCCTTCGAG 
GAGATCCGAG CTGAAGTGTT CCGGAAGAAA CTGAAGGAGC GAAGGGAAGC TGAACTGCTG 
ACCAGTGCAG AAAAGCGGGC AGAAATGCAG AAACAGATTG AGGAGATGGA GAGAAAGCTG 
AAGGAAATCC AGACTATTCA ACAAGAAAGA GCTGGTGACC AGCAAGAAGA GAAGGTGCCT 
ACAGAGGAGA CCACCAGACT GCAGATTCCT TCGGAGTCCC AGGAAAGGCT GGCAACATCT 
CTGTCCCGTT CTGTCTGTCC ACCAAGCTCT CACCCCAGGG AAACTTCACC AGCTAAAAAC 
ATTTTGCAAG AACAGCCTGA CTCTAAAGGT CCCAGCATGC CTTTCTCCAT TTTCGATGAG 
TTTCTTTCGG ACGACAAGGA CAAAAGTCTG GCTACAGATC TTCCACAGGT TCCCAAAGCC 
CAGCGAAGAC CCCTTGCTGT TCTCAAAACC ACGGAAATCA TTGCCTCAAA TGAGGAGATG 
TCTCCTGATG TTTGTGATGA ACTTACAGGA ATTGAACCTT TGAGTGAAGA CGCCATCATC 
ACAGGTTTCA GTAACGCGAC CATCTGTCCC AACCCCGAGG ACACTTGTGA CTTTGTTAGA 
GCAGCTCGTT TTGCGTCTAC TCCTTTCCCT GAGATACTGT CCTTGAAGGG GGTCCCTTCC 
GGTCCTGAGA GACTGTTGCA GGAAGAGGAT CTTGATGGCA AGACCACCGA GGGCTGTCTC 
ACCGCACGGA CCACTCTCTA TAACCAGACC CTCAGCGTCA AGAAGCTGAG CCCGATTATT 
GAAGACAGTC GGGAAGCCAC ACACTCCTCC GGCTTCTCTG GATCCTCCGC TTCAGTTACG 
AGTACCTCCT CCATCAAAGG CCTTCAGCTT CCCGAGAAGC TGGAGCTGAC TAATGACACC 
ACAGAAAATG CTATTCAGTC ACCCTGGTGC TCACAATATC GGCTACAACT ACTGAAGTCC 
CTACCAGAGT CAAGTGATTC TGCAGAGTTT TCTCTGGAAG ATAGACCAAT GCCGACCCTG 
GAGATAGGGG AGGAGATTGA GTTAGGTGAC GAAGACTACT GCATTAAACA GGAATACCTA 
GTATGTGAAG AGTACAAGTT ATTCTGGGCG AAATCTAGAA ACTCTGCAGA GTTAACCATG 
ATAAAGGCAT CTTTTCAACC TATCCCATGG GATTTTTATG TCAACCTCAA GTTAAAGGAA 
CGTCTAAACG AGGACTCTGA TCAGCTGTGC AGCTACTATC AGTACCAAGA CGGCCATATC 
GTTTCCTACC AATATGTCAA GTGTTGCACC CTTCAGGATC TTCTCCAGCA CAGTGAGTCT 
GTGTCTCATG AAGTAACCGT CTTGATTATT TATAACCTCT TGACAATAGT GGAGAAACTC 
CACAAAGCTG AAATAGTTCA TGGAGACCTG AGTCCAAGGA GTCTGATCCT AAGAAACAGA 
ATCCATGACC CCTATGATTG CAATAAGAAG GATCATGCTG TGAAGATCGT GGACTTCTCC 
TACAGTGTTG ACCTGAGGGT GCAGCTAGAT GCTTTTTCCT TCAGTGGCTT TCGGACTGTA 
CAGACCCTGG AAGGACAAAA GATCCTGGAG AACTGTTCTT CACCCTACCA GGTAGATCTG 
TTGGGCATAG CAGACCTAGC ACACTTACTG TTGTTCAAGG AACACCTGCA TGTCTTCTGG 
GATGGGCTTC TCTGGAGACT CAGCCAGACT ACTTCTGAGC TAAAGAATGG TGAATTATGG 
AATAAATTCT TTGTGCGGAT TCTGAATGCC AGCGATGAGC CCACAGTATT GGTTCTGAGG 
GAACTTGCAG CGGAAGTGAA CGGGGTTTTT GACACCACAT TCCACAGTCA CCTGGACAAA 
GCCTTGTGGA AGAAGGAAGT GAGGACTGCT GAGGGGCTAG AAGACGGTTT GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006979290.1
CDS	88..3384
Translation	MLLSRSTGRRSPQLQGQLQVEEGSSDSRHPEEKGLRTGESKNRECGMAAARKEGLAASEAMSLEGDEWELSKENVQPLRHGRIMSTLQGALAQQESACNTTLQQQKRAFESEIRFYSGNDPLDVWDRYITWTEQSYPQGGKESNMSTLLERAVEALQGETRYYSDPRFLSLWLKLGHLCNEPLDLYSYLHSQGIGVSLAQFYISWAEEYEARENFKRADAVFQEGIERKAEPLERLQSQHRQFQARVSRQALLALEKEEEEEVLGSSVPQRSTLAELKSRGKKTAKAPISRVGGALKAPGQSRGFQNIVPQQMCSNRRITVFDENADNSSKAELPKPVVQPWMAPPISKAKENELQPGPWNADRPLDYRPHGSSASVVAAPPSLPSFTPYVEESAQQTVMTPCKIEPSINHVLSTRKPGKEEGDPLQRVQSHQQGSEERKEKMMYCKDKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAEKRAEMQKQIEEMERKLKEIQTIQQERAGDQQEEKVPTEETTRLQIPSESQERLATSLSRSVCPPSSHPRETSPAKNILQEQPDSKGPSMPFSIFDEFLSDDKDKSLATDLPQVPKAQRRPLAVLKTTEIIASNEEMSPDVCDELTGIEPLSEDAIITGFSNATICPNPEDTCDFVRAARFASTPFPEILSLKGVPSGPERLLQEEDLDGKTTEGCLTARTTLYNQTLSVKKLSPIIEDSREATHSSGFSGSSASVTSTSSIKGLQLPEKLELTNDTTENAIQSPWCSQYRLQLLKSLPESSDSAEFSLEDRPMPTLEIGEEIELGDEDYCIKQEYLVCEEYKLFWAKSRNSAELTMIKASFQPIPWDFYVNLKLKERLNEDSDQLCSYYQYQDGHIVSYQYVKCCTLQDLLQHSESVSHEVTVLIIYNLLTIVEKLHKAEIVHGDLSPRSLILRNRIHDPYDCNKKDHAVKIVDFSYSVDLRVQLDAFSFSGFRTVQTLEGQKILENCSSPYQVDLLGIADLAHLLLFKEHLHVFWDGLLWRLSQTTSELKNGELWNKFFVRILNASDEPTVLVLRELAAEVNGVFDTTFHSHLDKALWKKEVRTAEGLEDGLA

Target ORF information:

RefSeq Version	XM_006979228.2
Organism	Peromyscus maniculatus bairdii(prairie deer mouse)
Definition	Peromyscus maniculatus bairdii BUB1 mitotic checkpoint serine/threonine kinase B (Bub1b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006979228.2

ORF Insert Sequence:

ATGCTTTTGT CTAGGAGCAC GGGGAGACGG TCTCCGCAAC TCCAGGGCCA GCTGCAGGTT 
GAGGAAGGAA GCTCGGACAG TCGGCACCCG GAGGAGAAAG GTCTGCGGAC GGGCGAGAGC 
AAGAATCGGG AATGCGGGAT GGCGGCGGCG AGGAAGGAAG GGCTTGCAGC GAGTGAAGCC 
ATGTCCCTGG AGGGAGATGA GTGGGAGCTG AGTAAAGAGA ACGTGCAACC CTTACGGCAT 
GGCCGGATCA TGTCCACGCT CCAGGGAGCT CTGGCACAGC AGGAATCAGC CTGCAACACT 
ACCCTTCAGC AACAGAAGCG GGCATTTGAA TCTGAAATCC GCTTTTACTC TGGAAATGAC 
CCTCTGGATG TTTGGGACAG GTATATTACC TGGACCGAGC AGAGCTACCC TCAAGGCGGG 
AAAGAGAGTA ACATGTCGAC GTTACTAGAA AGAGCCGTAG AAGCTCTACA AGGAGAGACG 
CGCTACTACA GTGACCCCCG GTTTCTCAGT CTCTGGCTCA AATTGGGGCA CCTGTGCAAC 
GAGCCCTTGG ATTTGTACAG CTATTTACAC AGCCAGGGGA TTGGCGTCTC TCTTGCCCAG 
TTCTACATCT CATGGGCAGA AGAATACGAA GCTAGAGAAA ACTTCAAGAG AGCGGACGCC 
GTGTTCCAGG AAGGGATTGA ACGCAAGGCT GAGCCCCTGG AAAGACTGCA GTCCCAGCAC 
AGACAATTCC AGGCTCGAGT GTCTCGACAA GCTCTCTTGG CACTTGAGAA GGAGGAGGAG 
GAGGAGGTCC TGGGCTCTTC TGTACCACAG AGAAGCACGC TTGCTGAGCT GAAGAGCCGA 
GGGAAGAAGA CAGCCAAGGC GCCCATCAGC CGTGTGGGAG GCGCTCTGAA GGCTCCGGGT 
CAGAGCAGAG GATTCCAAAA TATAGTTCCT CAGCAGATGT GCAGTAACCG CAGGATCACT 
GTTTTTGATG AAAACGCTGA TAACTCTTCG AAGGCAGAGT TACCTAAGCC TGTCGTCCAA 
CCATGGATGG CGCCCCCTAT CTCTAAGGCC AAAGAGAATG AGCTACAGCC AGGCCCGTGG 
AACGCAGACA GACCCTTGGA TTATAGGCCT CATGGCAGTT CGGCCTCCGT AGTAGCTGCG 
CCCCCTTCGC TTCCCAGCTT TACGCCCTAT GTGGAAGAGA GCGCTCAGCA GACAGTCATG 
ACACCATGTA AAATTGAACC TAGTATCAAC CATGTTCTCA GCACCAGAAA GCCTGGGAAG 
GAAGAAGGAG ACCCCTTGCA AAGGGTTCAG AGTCATCAGC AAGGGTCTGA GGAGAGGAAG 
GAGAAGATGA TGTACTGTAA GGACAAGATT TATGCTGGAG TCGGGGAGTT CTCCTTCGAG 
GAGATCCGAG CTGAAGTGTT CCGGAAGAAA CTGAAGGAGC GAAGGGAAGC TGAACTGCTG 
ACCAGTGCAG AAAAGCGGGC AGAAATGCAG AAACAGATTG AGGAGATGGA GAGAAAGCTG 
AAGGAAATCC AGACTATTCA ACAAGAAAGA GCTGGTGACC AGCAAGAAGA GAAGGTGCCT 
ACAGAGGAGA CCACCAGACT GCAGATTCCT TCGGAGTCCC AGGAAAGGCT GGCAACATCT 
CTGTCCCGTT CTGTCTGTCC ACCAAGCTCT CACCCCAGGG AAACTTCACC AGCTAAAAAC 
ATTTTGCAAG AACAGCCTGA CTCTAAAGGT CCCAGCATGC CTTTCTCCAT TTTCGATGAG 
TTTCTTTCGG ACGACAAGGA CAAAAGTCTG GCTACAGATC TTCCACAGGT TCCCAAAGCC 
CAGCGAAGAC CCCTTGCTGT TCTCAAAACC ACGGAAATCA TTGCCTCAAA TGAGGAGATG 
TCTCCTGATG TTTGTGATGA ACTTACAGGA ATTGAACCTT TGAGTGAAGA CGCCATCATC 
ACAGGTTTCA GTAACGCGAC CATCTGTCCC AACCCCGAGG ACACTTGTGA CTTTGTTAGA 
GCAGCTCGTT TTGCGTCTAC TCCTTTCCCT GAGATACTGT CCTTGAAGGG GGTCCCTTCC 
GGTCCTGAGA GACTGTTGCA GGAAGAGGAT CTTGATGGCA AGACCACCGA GGGCTGTCTC 
ACCGCACGGA CCACTCTCTA TAACCAGACC CTCAGCGTCA AGAAGCTGAG CCCGATTATT 
GAAGACAGTC GGGAAGCCAC ACACTCCTCC GGCTTCTCTG GATCCTCCGC TTCAGTTACG 
AGTACCTCCT CCATCAAAGG CCTTCAGCTT CCCGAGAAGC TGGAGCTGAC TAATGACACC 
ACAGAAAATG CTATTCAGTC ACCCTGGTGC TCACAATATC GGCTACAACT ACTGAAGTCC 
CTACCAGAGT CAAGTGATTC TGCAGAGTTT TCTCTGGAAG ATAGACCAAT GCCGACCCTG 
GAGATAGGGG AGGAGATTGA GTTAGGTGAC GAAGACTACT GCATTAAACA GGAATACCTA 
GTATGTGAAG AGTACAAGTT ATTCTGGGCG AAATCTAGAA ACTCTGCAGA GTTAACCATG 
ATAAAGGCAT CTTTTCAACC TATCCCATGG GATTTTTATG TCAACCTCAA GTTAAAGGAA 
CGTCTAAACG AGGACTCTGA TCAGCTGTGC AGCTACTATC AGTACCAAGA CGGCCATATC 
GTTTCCTACC AATATGTCAA GTGTTGCACC CTTCAGGATC TTCTCCAGCA CAGTGAGTCT 
GTGTCTCATG AAGTAACCGT CTTGATTATT TATAACCTCT TGACAATAGT GGAGAAACTC 
CACAAAGCTG AAATAGTTCA TGGAGACCTG AGTCCAAGGA GTCTGATCCT AAGAAACAGA 
ATCCATGACC CCTATGATTG CAATAAGAAG GATCATGCTG TGAAGATCGT GGACTTCTCC 
TACAGTGTTG ACCTGAGGGT GCAGCTAGAT GCTTTTTCCT TCAGTGGCTT TCGGACTGTA 
CAGACCCTGG AAGGACAAAA GATCCTGGAG AACTGTTCTT CACCCTACCA GGTAGATCTG 
TTGGGCATAG CAGACCTAGC ACACTTACTG TTGTTCAAGG AACACCTGCA TGTCTTCTGG 
GATGGGCTTC TCTGGAGACT CAGCCAGACT ACTTCTGAGC TAAAGAATGG TGAATTATGG 
AATAAATTCT TTGTGCGGAT TCTGAATGCC AGCGATGAGC CCACAGTATT GGTTCTGAGG 
GAACTTGCAG CGGAAGTGAA CGGGGTTTTT GACACCACAT TCCACAGTCA CCTGGACAAA 
GCCTTGTGGA AGAAGGAAGT GAGGACTGCT GAGGGGCTAG AAGACGGTTT GGCTTGA