NIT1 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	NIT1
Entrez Gene ID	745973
Full Name	nitrilase 1
Synonyms	DEDD
General protein information	Preferred Names nitrilase 1 Names nitrilase homolog 1 death effector domain containing
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001152578.4	XP_001152578.1	deaminated glutathione amidase

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nit1	NP_001004638.1
Mus musculus (house mouse)	Nit1	NP_036179.1
Rattus norvegicus (Norway rat)	Nit1	NP_872609.2
Gallus gallus (chicken)	NIT1	XP_003642751.1
Saccharomyces cerevisiae (baker's yeast)	NIT2	NP_012409.1
Homo sapiens (human)	NIT1	NP_005591.1
	NIT1	XP_001115539.1
Schizosaccharomyces pombe (fission yeast)	SPBC651.02	NP_595500.1
Neurospora crassa	NCU05757	XP_959732.2
Oryza sativa (rice)	Os12g0502500	NP_001066830.1
Pan troglodytes (chimpanzee)	NIT1	XP_001152578.1
Canis lupus familiaris (dog)	NIT1	XP_536134.3
Arabidopsis thaliana (thale cress)	AT4G08790	NP_567340.1
Bos taurus (cattle)	NIT1	NP_001033112.1
Kluyveromyces lactis	KLLA0E15247g	XP_454637.1
Xenopus tropicalis (tropical clawed frog)	nit1	XP_004919403.1

Gene Ontology

The following NIT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NIT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPc03662	XM_001152578.4 Latest version!	Pan troglodytes nitrilase 1 (NIT1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NIT1 ( XM_001152578.4 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_001152578.1 Pan troglodytes nitrilase 1 (NIT1), mRNA.

Price & Availability↑

CloneID	OPc03662
Clone ID Related Accession (Same CDS sequence)	XM_001152578.4
Accession Version	XM_001152578.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 984bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	deaminated glutathione amidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_001152578.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCGTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAACGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCGTTACAG GCCCAGCCCA CTGGGAGGTG 
TTGCTGCGGG CCCGTGCTAT CGAAACCCAG TGCTATGTAG TGGCAGCGGC ACAGTGTGGA 
CGCCACCATG AGAAGAGAGC AAGTTATGGC CACAGCATGG TGGTAGACCC CTGGGGAACA 
GTGGTGGCCC GCTGCTCGGA GGGGCCAGGC CTCTGCCTTG CCCGAATAGA CCTCAACTAT 
CTGCGACAGT TGCGCCAACA CCTGCCTGTG TTCCAGCACC GCAGGCCTGA CCTCTATGGC 
AATCTGGGTC ACCCACTGTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001152578.1
CDS	101..1084
Translation	MLGFITRPPHRFLSVLCPGLRIPQLSVLCAQPRPRAMAISSSSCELPLVAVCQVTSTPDKQQNFKTCAELVREAARLGACLAFLPEAFDFIARDPAETLHLSEPLGGKLLEEYTQLARECGLWLSLGGFHERGQDWEQTQKIYNCHVLLNSNGAVVATYRKTHLCDVEIPGQGPMCESNSTMPGPSLESPVSTPAGKIGLAVCYDMRFPELSLALAQAGAEILTYPSAFGSVTGPAHWEVLLRARAIETQCYVVAAAQCGRHHEKRASYGHSMVVDPWGTVVARCSEGPGLCLARIDLNYLRQLRQHLPVFQHRRPDLYGNLGHPLS

Target ORF information:

RefSeq Version	XM_001152578.4
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes nitrilase 1 (NIT1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001152578.4

ORF Insert Sequence:

ATGCTGGGCT TCATCACCAG GCCTCCTCAC AGATTCCTGT CCGTTCTGTG TCCTGGACTC 
CGGATACCTC AACTCTCAGT ACTTTGTGCT CAGCCCAGGC CCAGAGCCAT GGCTATCTCC 
TCTTCCTCCT GCGAACTGCC CCTGGTGGCT GTGTGCCAGG TAACATCGAC GCCAGACAAG 
CAACAGAACT TTAAAACATG TGCTGAGCTG GTTCGAGAGG CTGCCAGACT GGGTGCCTGC 
CTGGCTTTCC TGCCTGAGGC ATTTGACTTC ATTGCACGGG ACCCTGCAGA GACGCTACAC 
CTGTCTGAAC CACTGGGTGG GAAACTTTTG GAAGAATACA CCCAGCTTGC CAGGGAATGT 
GGACTCTGGC TGTCCTTGGG TGGTTTCCAT GAGCGTGGCC AAGACTGGGA GCAGACTCAG 
AAAATCTACA ATTGTCACGT GCTGCTGAAC AGCAACGGGG CAGTAGTGGC CACTTACAGG 
AAGACACATC TGTGTGACGT AGAGATTCCA GGGCAGGGGC CTATGTGTGA AAGCAACTCT 
ACCATGCCTG GGCCCAGTCT TGAGTCACCT GTCAGCACAC CAGCAGGCAA GATTGGTCTA 
GCTGTCTGCT ATGACATGCG GTTCCCTGAA CTCTCTCTGG CATTGGCTCA AGCTGGAGCA 
GAGATACTTA CCTATCCTTC AGCTTTTGGA TCCGTTACAG GCCCAGCCCA CTGGGAGGTG 
TTGCTGCGGG CCCGTGCTAT CGAAACCCAG TGCTATGTAG TGGCAGCGGC ACAGTGTGGA 
CGCCACCATG AGAAGAGAGC AAGTTATGGC CACAGCATGG TGGTAGACCC CTGGGGAACA 
GTGGTGGCCC GCTGCTCGGA GGGGCCAGGC CTCTGCCTTG CCCGAATAGA CCTCAACTAT 
CTGCGACAGT TGCGCCAACA CCTGCCTGTG TTCCAGCACC GCAGGCCTGA CCTCTATGGC 
AATCTGGGTC ACCCACTGTC TTAA