IARS2 cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	IARS2
Entrez Gene ID	457749
Full Name	isoleucyl-tRNA synthetase 2, mitochondrial
General protein information	Preferred Names isoleucyl-tRNA synthetase 2, mitochondrial Names isoleucyl-tRNA synthetase, mitochondrial isoleucine--tRNA ligase, mitochondrial mitochondrial isoleucine tRNA synthetase
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_514209.6	XP_514209.2	isoleucine--tRNA ligase, mitochondrial
XM_514209.5	XP_514209.2	isoleucine--tRNA ligase, mitochondrial

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG5414	NP_648837.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003156	XP_312846.5
Eremothecium gossypii	AGOS_AGL247C	NP_986420.1
Neurospora crassa	NCU00920	XP_964746.2
Arabidopsis thaliana (thale cress)	OVA2	NP_199714.2
Canis lupus familiaris (dog)	IARS2	XP_536121.3
Gallus gallus (chicken)	IARS2	NP_001006397.1
Saccharomyces cerevisiae (baker's yeast)	ISM1	NP_015285.1
Schizosaccharomyces pombe (fission yeast)	SPCC18B5.08c	NP_587938.1
Homo sapiens (human)	IARS2	NP_060530.3
Pan troglodytes (chimpanzee)	IARS2	XP_514209.2
	IARS2	XP_001099877.1
Danio rerio (zebrafish)	iars2	NP_001157270.1
Caenorhabditis elegans (roundworm)	iars-2	NP_001251317.1
Kluyveromyces lactis	KLLA0D11858g	XP_453590.1
Xenopus tropicalis (tropical clawed frog)	iars2	NP_001120515.1
Bos taurus (cattle)	IARS2	NP_001193724.1
Mus musculus (house mouse)	Iars2	NP_941055.1
Rattus norvegicus (Norway rat)	Iars2	XP_006250494.1
Magnaporthe oryzae (rice blast fungus)	MGG_01165	XP_003714047.1
Oryza sativa (rice)	Os02g0778200	NP_001048293.1

Gene Ontology

The following IARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPc04187	XM_514209.6 Latest version!	Pan troglodytes isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPc60983	XM_514209.5 Latest version!	Pan troglodytes isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IARS2 ( XM_514209.6 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_514209.2 Pan troglodytes isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Price & Availability↑

CloneID	OPc04187
Clone ID Related Accession (Same CDS sequence)	XM_514209.6
Accession Version	XM_514209.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036879.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_514209.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CTCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAATGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCACTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTC 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACAGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTC CCCATGGATT GTTTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CATATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACAGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGACG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACAAAG 
GATGAATACT TGATCAACAG CCAAACCATT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAGCAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGTG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGCTGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTTAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAGCAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATG GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTC GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCATTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCTCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_514209.2
CDS	111..3149
Translation	MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTIEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPAVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVMVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVIIVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

Target ORF information:

RefSeq Version	XM_514209.6
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_514209.6

ORF Insert Sequence:

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CTCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAATGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCACTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTC 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACAGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTC CCCATGGATT GTTTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CATATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACAGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGACG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACAAAG 
GATGAATACT TGATCAACAG CCAAACCATT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAGCAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGTG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGCTGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTTAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAGCAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATG GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTC GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCATTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCTCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IARS2 ( XM_514209.5 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_514209.2 Pan troglodytes isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Price & Availability↑

CloneID	OPc60983
Clone ID Related Accession (Same CDS sequence)	XM_514209.5
Accession Version	XM_514209.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	isoleucine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006468.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 2, 2016 this sequence version replaced XM_514209.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CTCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAATGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCACTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTC 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACAGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTC CCCATGGATT GTTTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CGTATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACAGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGACG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACAAAG 
GATGAATACT TGATCAACAG CCAAACCATT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAGCAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGTG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGCTGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTTAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAGCAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATG GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTC GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCATTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCTCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_514209.2
CDS	111..3149
Translation	MRWGLRPRGPGAAALATARSLWGTPRLPCSPGWQGATKRLLVRSVSGASNHQPNSNSGRYRDTVLLPQTSFPMKLLGRQQPDTELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFHMMNGSKIHFVPGWDCHGLPIEIKVLSELGREAQNLSAMEIRKKARSFAKAAIEKQKSAFIRWGIMADWNNCYYTFDGKYEAKQLRTFYQMYDKGLVYRSYKPVFWSPSSRTALAEAELEYNPEHVSRSIYVKFPLLKPSPKLASLIDGSSPVSILVWTTQPWTIPANEAVCYMPESKYAVVKCSKSGDLYVLAADKVASVASTLETTFETISTLSGVDLENGTCSHPLIPDKASPLLPANHVTMAKGTGLVHTAPAHGMEDYGVASQHNLPMDCLVDEDGVFTDVAGPELQNKAVLEEGTDVVIKMLQTAKNLLKEEKLVHSYPYDWRTKKPVVIRASKQWFINITDIKTAAKELLKKVKFIPGSALNGMVEMMDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTIEHIVKLVEQHGSDIWWTLPPEQLLPKEVLSEVGGPDALEYVPGQDILDIWFDSGTSWSYVLPGPDQRADLYLEGKDQLGGWFQSSLLTSVAARKRAPYKTVIVHGFTLGEKGEKMSKSLGNVIHPAVVVNGGQDQSKEPPYGADVLRWWVADSNVFTEVAIGPSVLNAARDDISKLRNTLRFLLGNVADFNPETDSIPVNDMYVIDQYMLHLLQDLANKITELYKQYDFGKVVRLLRTFYTRELSNFYFSIIKDRLYCEKENDPKRRSCQTALVEILDVMVRSFAPILPHLAEEVFQHIPYIKEPKSVFRTGWISTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYKVIIVIEPGLLFEIIEMLQSEETSSTSQLNELMMASESTLLAQEPREMTADVIELKGKFLINLEGGDIREESSYKVIVMPTTKEKCPRCWKYTAESSDTLCPRCAEVVSGK

Target ORF information:

RefSeq Version	XM_514209.5
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes isoleucyl-tRNA synthetase 2, mitochondrial (IARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_514209.5

ORF Insert Sequence:

ATGCGTTGGG GGCTGCGCCC TCGCGGGCCG GGCGCGGCCG CCCTGGCCAC TGCCCGAAGT 
TTGTGGGGGA CTCCCCGCCT TCCCTGCAGC CCGGGATGGC AAGGGGCGAC GAAGAGGCTT 
CTGGTGCGGT CGGTCTCCGG GGCCAGTAAC CACCAGCCGA ACTCGAATAG TGGCAGATAC 
CGGGACACGG TGCTGCTGCC GCAGACGAGC TTCCCCATGA AGCTGCTGGG CCGCCAGCAG 
CCGGACACGG AGCTGGAGAT CCAGCAGAAA TGTGGATTTT CAGAACTTTA TTCATGGCAA 
AGAGAAAGAA AAGTAAAGAC AGAATTTTGC CTTCATGATG GACCTCCTTA TGCAAATGGT 
GACCCTCATG TTGGACATGC TTTAAATAAG ATTTTGAAAG ACATAGCCAA TCGATTCCAT 
ATGATGAATG GCTCCAAAAT ACATTTTGTG CCCGGCTGGG ATTGTCATGG GTTGCCCATT 
GAAATAAAAG TATTATCAGA ACTTGGTAGA GAAGCTCAGA ATCTTTCAGC TATGGAAATT 
AGAAAGAAAG CTAGATCATT TGCTAAAGCA GCCATTGAGA AACAGAAATC AGCATTTATT 
CGTTGGGGAA TAATGGCAGA TTGGAATAAT TGCTACTATA CATTTGATGG GAAGTATGAA 
GCCAAACAGT TGAGAACTTT TTACCAAATG TATGATAAGG GCTTGGTTTA TCGATCTTAC 
AAACCTGTGT TTTGGTCTCC GTCATCTAGG ACTGCACTGG CTGAAGCAGA ACTTGAATAT 
AATCCTGAGC ATGTCAGTCG TTCAATATAT GTAAAATTTC CTCTCTTAAA GCCTTCTCCA 
AAATTGGCAT CTCTTATAGA TGGTTCATCT CCTGTTAGTA TTTTGGTCTG GACCACACAA 
CCTTGGACGA TTCCAGCCAA TGAAGCTGTT TGCTATATGC CTGAATCAAA GTATGCTGTC 
GTGAAATGTT CTAAGTCTGG AGACCTCTAC GTACTGGCGG CAGATAAAGT AGCATCTGTT 
GCTTCTACTT TGGAAACAAC ATTTGAGACT ATTTCAACAC TTTCAGGTGT AGATTTGGAA 
AATGGTACTT GCAGTCATCC ATTAATTCCT GATAAAGCCT CTCCTCTTTT ACCTGCAAAT 
CATGTGACCA TGGCAAAAGG AACAGGATTG GTTCACACAG CCCCAGCTCA TGGTATGGAA 
GACTACGGTG TAGCGTCTCA GCACAACCTC CCCATGGATT GTTTAGTGGA CGAAGATGGA 
GTTTTCACAG ATGTTGCAGG TCCTGAACTT CAAAACAAGG CTGTCCTTGA AGAGGGAACT 
GATGTGGTTA TAAAGATGCT TCAGACTGCA AAGAATTTGT TGAAAGAGGA GAAATTGGTG 
CATAGCTATC CGTATGACTG GAGGACCAAG AAACCTGTGG TTATTCGTGC CAGCAAGCAG 
TGGTTTATAA ACATCACAGA TATTAAGACT GCAGCCAAGG AATTGTTAAA AAAGGTGAAA 
TTTATTCCTG GATCAGCACT GAATGGCATG GTTGAAATGA TGGACAGACG GCCATATTGG 
TGTATATCAA GGCAAAGAGT TTGGGGTGTT CCAATTCCTG TGTTTCATCA TAAGACAAAG 
GATGAATACT TGATCAACAG CCAAACCATT GAGCATATTG TTAAACTAGT GGAACAACAC 
GGCAGTGATA TCTGGTGGAC TCTTCCCCCT GAGCAACTTC TTCCAAAAGA AGTCTTATCT 
GAGGTTGGTG GCCCTGATGC CTTGGAATAT GTGCCAGGTC AGGATATTTT GGACATCTGG 
TTTGATAGTG GAACTTCATG GTCTTATGTT CTTCCAGGTC CTGACCAAAG AGCAGATTTG 
TACTTGGAAG GAAAAGACCA GCTCGGGGGT TGGTTTCAGT CATCCTTATT AACAAGTGTG 
GCAGCAAGGA AGAGAGCACC TTATAAGACA GTGATTGTTC ATGGATTTAC CCTTGGAGAA 
AAGGGAGAAA AGATGTCCAA GTCTCTTGGG AATGTCATTC ATCCTGCTGT TGTCGTTAAT 
GGAGGACAAG ATCAAAGCAA AGAGCCTCCG TATGGTGCTG ATGTCCTTCG CTGGTGGGTA 
GCTGATTCCA ATGTCTTCAC CGAAGTTGCA ATTGGCCCAT CCGTGCTCAA TGCTGCCAGA 
GATGATATTA GCAAGCTTAG GAATACACTT CGCTTTCTTT TGGGAAATGT GGCTGATTTC 
AACCCAGAAA CAGATTCCAT CCCTGTTAAC GATATGTATG TCATAGACCA GTACATGCTA 
CACTTACTGC AGGATTTGGC AAACAAGATT ACCGAATTAT ACAAGCAATA TGATTTTGGA 
AAAGTTGTTC GGCTGTTACG GACGTTTTAT ACCAGAGAGC TCTCTAACTT TTATTTCAGT 
ATAATCAAAG ATAGGCTCTA TTGTGAAAAG GAAAATGACC CCAAACGACG CTCTTGTCAG 
ACTGCATTAG TTGAAATTTT GGATGTAATG GTTCGTTCTT TTGCTCCCAT TCTTCCTCAC 
CTGGCTGAAG AGGTGTTCCA GCACATACCT TATATTAAAG AGCCCAAGAG TGTTTTCCGT 
ACTGGGTGGA TTAGTACTAG TTCTATCTGG AAAAAGCCCG GGTTGGAAGA AGCTGTGGAG 
AGTGCGTGTG CAATGCGAGA CTCATTTCTC GGAAGCATCC CTGGCAAAAA TGCAGCTGAG 
TACAAGGTTA TCATTGTGAT AGAACCTGGA CTGCTTTTTG AGATAATAGA GATGCTGCAG 
TCTGAAGAGA CTTCCAGCAC CTCTCAGTTG AATGAATTAA TGATGGCTTC TGAGTCAACT 
TTACTGGCTC AGGAACCACG AGAGATGACT GCAGATGTAA TCGAGCTTAA AGGGAAATTC 
CTCATCAACT TAGAAGGTGG TGATATTCGT GAAGAGTCTT CCTATAAAGT AATTGTCATG 
CCGACTACGA AAGAAAAATG CCCTCGTTGT TGGAAGTATA CAGCGGAGTC TTCAGATACA 
CTGTGTCCTC GATGTGCAGA AGTTGTCAGT GGAAAATAG