spout1 cDNA ORF clone, Oncorhynchus mykiss(rainbow trout)

Gene
Clones
gRNAs

Summary

Gene Symbol	spout1
Entrez Gene ID	110523306
Full Name	SPOUT domain containing methyltransferase 1
Gene Type	protein-coding
Organism	Oncorhynchus mykiss(rainbow trout)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021601895.1	XP_021457570.1	putative methyltransferase C9orf114 homolog isoform X1
XM_021601896.1	XP_021457571.1	putative methyltransferase C9orf114 homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following spout1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spout1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOf44031	XM_021601895.1 Latest version!	Oncorhynchus mykiss SPOUT domain containing methyltransferase 1 (spout1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OOf44032	XM_021601896.1 Latest version!	Oncorhynchus mykiss SPOUT domain containing methyltransferase 1 (spout1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spout1 ( XM_021601895.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021457570.1 Oncorhynchus mykiss SPOUT domain containing methyltransferase 1 (spout1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOf44031
Clone ID Related Accession (Same CDS sequence)	XM_021601895.1
Accession Version	XM_021601895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	putative methyltransferase C9orf114 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACA GCGTTGCCTC GAAAAAGCCC AAATTGTCCT CTTCTCAGGG CGAGGACAGA 
GTAGATTGGA AGAAATGGAA AGCGGAACGA AAAGAGATCA AGAAGCAAAT CAAAGAATCC 
AAGCTGATTC AACAACTTGA CAAGCAGAAG CAAGAGGAGG AGGAGAAGAC TGAGGCAGCA 
CTGCAGCAGA GTCAGAGGGA AAACCGATCA GGACGGTCGT ACACAGTGAG CGTGGCCTTG 
CCTGGTTCTG TCCTGGACAA TGCCCAGTCT ACAGAACTCC GCACATACCT GGCTGGACAG 
ATAGCCAGAG CCTGCGTCGT GTTCTGTGTC GACGAGATCA TCGTATTTGA CGAACAAGAT 
GAGGATGTCA AGACTGTTGA GGGAGAATTC AATGGTGTCG GCAAGAAGGG TCAAGCCTGC 
ATTCAACTGG CTAGAATCCT CCAGTTCTTG GAATGCCCAC AGTACCTGCG CAAATCATTC 
TTCCCAATGC ATAAAGATTT ACAGTATGCC GGCTTGCTCA ACCCTCTGGA CAGCCCTCAC 
CACATGAGGA AGGATGATGA GTGTGAGTAC CGGGAGGGGG TGGTCCTCGA TCGGCCTAGC 
AAACCAGGAA AAGGCTCCTT GGTCAACTGT GGAATGTGGA AGGAGGTGCA GATAGATAAG 
CAGCTACAGT CTGGTCTCAG AGTCACTGTC CACATGAACA AGATCCAGAA TAAAGATGGT 
AGACTACACA AAGGGGTGGT GGTGGCGCCT CACAAGCCGC GAACAGAGGG AGGTCTGTAC 
TGGGGCTACA GTGTTCGCCT GGCCTCAAGT CTAAGTAATG TGTTCACCGA GTGTCCACAT 
AAAGAGGGCT ATGATCTGAC CATTGGAACA TCTGAGAAAG GCAGTGATGT TGACAAAACT 
TCTCTTTCTC CATTCAAGCA CATGTTGGTT GTGTTTGGTG GTCTGCAGGG CTTGGAGGCC 
AGTGTGGATG CTGATCAGAA CCTGGAGGTG GCGGACCCCG GTGTCCTATT TGACCTGTAC 
CTCAACACCT GCCCCAACCA GGGCAGCAGA ACCATCCGCA CAGAGGAGGC CATCCTGATC 
TCCATGTCCA GCCTGAGGCA GAAAATCACA GCCGCATTTC CAGACAAGTC GAACGGTTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021457570.1
CDS	56..1198
Translation	MTDSVASKKPKLSSSQGEDRVDWKKWKAERKEIKKQIKESKLIQQLDKQKQEEEEKTEAALQQSQRENRSGRSYTVSVALPGSVLDNAQSTELRTYLAGQIARACVVFCVDEIIVFDEQDEDVKTVEGEFNGVGKKGQACIQLARILQFLECPQYLRKSFFPMHKDLQYAGLLNPLDSPHHMRKDDECEYREGVVLDRPSKPGKGSLVNCGMWKEVQIDKQLQSGLRVTVHMNKIQNKDGRLHKGVVVAPHKPRTEGGLYWGYSVRLASSLSNVFTECPHKEGYDLTIGTSEKGSDVDKTSLSPFKHMLVVFGGLQGLEASVDADQNLEVADPGVLFDLYLNTCPNQGSRTIRTEEAILISMSSLRQKITAAFPDKSNGS

Target ORF information:

RefSeq Version	XM_021601895.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss SPOUT domain containing methyltransferase 1 (spout1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021601895.1

ORF Insert Sequence:

ATGACGGACA GCGTTGCCTC GAAAAAGCCC AAATTGTCCT CTTCTCAGGG CGAGGACAGA 
GTAGATTGGA AGAAATGGAA AGCGGAACGA AAAGAGATCA AGAAGCAAAT CAAAGAATCC 
AAGCTGATTC AACAACTTGA CAAGCAGAAG CAAGAGGAGG AGGAGAAGAC TGAGGCAGCA 
CTGCAGCAGA GTCAGAGGGA AAACCGATCA GGACGGTCGT ACACAGTGAG CGTGGCCTTG 
CCTGGTTCTG TCCTGGACAA TGCCCAGTCT ACAGAACTCC GCACATACCT GGCTGGACAG 
ATAGCCAGAG CCTGCGTCGT GTTCTGTGTC GACGAGATCA TCGTATTTGA CGAACAAGAT 
GAGGATGTCA AGACTGTTGA GGGAGAATTC AATGGTGTCG GCAAGAAGGG TCAAGCCTGC 
ATTCAACTGG CTAGAATCCT CCAGTTCTTG GAATGCCCAC AGTACCTGCG CAAATCATTC 
TTCCCAATGC ATAAAGATTT ACAGTATGCC GGCTTGCTCA ACCCTCTGGA CAGCCCTCAC 
CACATGAGGA AGGATGATGA GTGTGAGTAC CGGGAGGGGG TGGTCCTCGA TCGGCCTAGC 
AAACCAGGAA AAGGCTCCTT GGTCAACTGT GGAATGTGGA AGGAGGTGCA GATAGATAAG 
CAGCTACAGT CTGGTCTCAG AGTCACTGTC CACATGAACA AGATCCAGAA TAAAGATGGT 
AGACTACACA AAGGGGTGGT GGTGGCGCCT CACAAGCCGC GAACAGAGGG AGGTCTGTAC 
TGGGGCTACA GTGTTCGCCT GGCCTCAAGT CTAAGTAATG TGTTCACCGA GTGTCCACAT 
AAAGAGGGCT ATGATCTGAC CATTGGAACA TCTGAGAAAG GCAGTGATGT TGACAAAACT 
TCTCTTTCTC CATTCAAGCA CATGTTGGTT GTGTTTGGTG GTCTGCAGGG CTTGGAGGCC 
AGTGTGGATG CTGATCAGAA CCTGGAGGTG GCGGACCCCG GTGTCCTATT TGACCTGTAC 
CTCAACACCT GCCCCAACCA GGGCAGCAGA ACCATCCGCA CAGAGGAGGC CATCCTGATC 
TCCATGTCCA GCCTGAGGCA GAAAATCACA GCCGCATTTC CAGACAAGTC GAACGGTTCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spout1 ( XM_021601896.1 ) cDNA ORF clone, Oncorhynchus mykiss(rainbow trout) -> XP_021457571.1 Oncorhynchus mykiss SPOUT domain containing methyltransferase 1 (spout1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOf44032
Clone ID Related Accession (Same CDS sequence)	XM_021601896.1
Accession Version	XM_021601896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1047bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-26
Organism	Oncorhynchus mykiss(rainbow trout)
Product	putative methyltransferase C9orf114 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_035081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Oncorhynchus mykiss Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGACA GCGTTGCCTC GAAAAAGCCC AAATTGTCCT CTTCTCAGGG CGAGGACAGA 
GTAGATTGGA AGAAATGGAA AGCGGAACGA AAAGAGATCA AGAAGCAAAT CAAAGAATCC 
AAGCTGATTC AACAACTTGA CAAGCAGAAG CAAGAGGAGG AGGAGAAGAC TGAGGCAGCA 
CTGCAGCAGA GTCAGAGGGA AAACCGATCA GGACGGTCGT ACACAGTGAG CGTGGCCTTG 
CCTGGTTCTG TCCTGGACAA TGCCCAGTCT ACAGAACTCC GCACATACCT GGCTGGACAG 
ATAGCCAGAG CCTGCGTCGT GTTCTGTGTC GACGAGATCA TCGTATTTGA CGAACAAGAT 
GAGGATGTCA AGACTGTTGA GGGAGAATTC AATGGTGTCG GCAAGAAGGG TCAAGCCTGC 
ATTCAACTGG CTAGAATCCT CCAGTTCTTG GAATGCCCAC AGTACCTGCG CAAATCATTC 
TTCCCAATGC ATAAAGATTT ACAGTATGCC GGCTTGCTCA ACCCTCTGGA CAGCCCTCAC 
CACATGAGGA AGGATGATGA GTGTGAGTAC CGGGAGGGGG TGGTCCTCGA TCGGCCTAGC 
AAACCAGGAA AAGGCTCCTT GGTCAACTGT GGAATGTGGA AGGAGGTGCA GATAGATAAG 
CAGCTACAGT CTGGTCTCAG AGTCACTGTC CACATGAACA AGATCCAGAA TAAAGATGGT 
AGACTACACA AAGGGGTGGT GGTGGCGCCT CACAAGCCGC GAACAGAGGG AGGTCTGTAC 
TGGGGCTACA GTGTTCGCCT GGCCTCAAGT CTAAGTAATG TGTTCACCGA GTGTCCACAT 
AAAGAGGGCT ATGATCTGAC CATTGGAACA TCTGAGAAAG GCAGTGATGT TGACAAAACT 
TCTCTTTCTC CATTCAAGGC TTGGAGGCCA GTGTGGATGC TGATCAGAAC CTGGAGGTGG 
CGGACCCCGG TGTCCTATTT GACCTGTACC TCAACACCTG CCCCAACCAG GGCAGCAGAA 
CCATCCGCAC AGAGGAGGCC ATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021457571.1
CDS	57..1103
Translation	MTDSVASKKPKLSSSQGEDRVDWKKWKAERKEIKKQIKESKLIQQLDKQKQEEEEKTEAALQQSQRENRSGRSYTVSVALPGSVLDNAQSTELRTYLAGQIARACVVFCVDEIIVFDEQDEDVKTVEGEFNGVGKKGQACIQLARILQFLECPQYLRKSFFPMHKDLQYAGLLNPLDSPHHMRKDDECEYREGVVLDRPSKPGKGSLVNCGMWKEVQIDKQLQSGLRVTVHMNKIQNKDGRLHKGVVVAPHKPRTEGGLYWGYSVRLASSLSNVFTECPHKEGYDLTIGTSEKGSDVDKTSLSPFKAWRPVWMLIRTWRWRTPVSYLTCTSTPAPTRAAEPSAQRRPS

Target ORF information:

RefSeq Version	XM_021601896.1
Organism	Oncorhynchus mykiss(rainbow trout)
Definition	Oncorhynchus mykiss SPOUT domain containing methyltransferase 1 (spout1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021601896.1

ORF Insert Sequence:

ATGACGGACA GCGTTGCCTC GAAAAAGCCC AAATTGTCCT CTTCTCAGGG CGAGGACAGA 
GTAGATTGGA AGAAATGGAA AGCGGAACGA AAAGAGATCA AGAAGCAAAT CAAAGAATCC 
AAGCTGATTC AACAACTTGA CAAGCAGAAG CAAGAGGAGG AGGAGAAGAC TGAGGCAGCA 
CTGCAGCAGA GTCAGAGGGA AAACCGATCA GGACGGTCGT ACACAGTGAG CGTGGCCTTG 
CCTGGTTCTG TCCTGGACAA TGCCCAGTCT ACAGAACTCC GCACATACCT GGCTGGACAG 
ATAGCCAGAG CCTGCGTCGT GTTCTGTGTC GACGAGATCA TCGTATTTGA CGAACAAGAT 
GAGGATGTCA AGACTGTTGA GGGAGAATTC AATGGTGTCG GCAAGAAGGG TCAAGCCTGC 
ATTCAACTGG CTAGAATCCT CCAGTTCTTG GAATGCCCAC AGTACCTGCG CAAATCATTC 
TTCCCAATGC ATAAAGATTT ACAGTATGCC GGCTTGCTCA ACCCTCTGGA CAGCCCTCAC 
CACATGAGGA AGGATGATGA GTGTGAGTAC CGGGAGGGGG TGGTCCTCGA TCGGCCTAGC 
AAACCAGGAA AAGGCTCCTT GGTCAACTGT GGAATGTGGA AGGAGGTGCA GATAGATAAG 
CAGCTACAGT CTGGTCTCAG AGTCACTGTC CACATGAACA AGATCCAGAA TAAAGATGGT 
AGACTACACA AAGGGGTGGT GGTGGCGCCT CACAAGCCGC GAACAGAGGG AGGTCTGTAC 
TGGGGCTACA GTGTTCGCCT GGCCTCAAGT CTAAGTAATG TGTTCACCGA GTGTCCACAT 
AAAGAGGGCT ATGATCTGAC CATTGGAACA TCTGAGAAAG GCAGTGATGT TGACAAAACT 
TCTCTTTCTC CATTCAAGGC TTGGAGGCCA GTGTGGATGC TGATCAGAAC CTGGAGGTGG 
CGGACCCCGG TGTCCTATTT GACCTGTACC TCAACACCTG CCCCAACCAG GGCAGCAGAA 
CCATCCGCAC AGAGGAGGCC ATCCTGA