NLRP3 cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus)

Gene
Clones
gRNAs

Summary

Gene Symbol	NLRP3
Entrez Gene ID	101380612
Full Name	NLR family pyrin domain containing 3
Gene Type	protein-coding
Organism	Odobenus rosmarus divergens(Pacific walrus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004407072.2	XP_004407129.1	NACHT, LRR and PYD domains-containing protein 3 isoform X1
XM_012564736.1	XP_012420190.1	NACHT, LRR and PYD domains-containing protein 3 isoform X2

Pathways from BioSystems
Gene Ontology

The following NLRP3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NLRP3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OOd60823	XM_012564736.1 Latest version!	Odobenus rosmarus divergens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OOd60822	XM_004407072.2 Latest version!	Odobenus rosmarus divergens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NLRP3 ( XM_012564736.1 ) cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus) -> XP_012420190.1 Odobenus rosmarus divergens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OOd60823
Clone ID Related Accession (Same CDS sequence)	XM_012564736.1
Accession Version	XM_012564736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Odobenus rosmarus divergens(Pacific walrus)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004450584.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Odobenus rosmarus divergens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATGG CAAGTGTCCG TTGCAAGCTG GCCCGTTACC TGGAGGATCT GGAGGATGCA 
GACTTTAAGA AGTTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG GTGCAGCCCA 
CTCCCTCGGA GTCAGACAGA GAAAGCTGAC CACATGGATC TGGCCACTCT GATGATTGAC 
TTCAACGGTG AGGAGAAGGC ATGGGCCATG GCAGTGTGGA TTTTTGCTGC AATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAACCAGAGT GGGATAATGC ACATGTGGTA 
TGCCGGGAAG AAAGCCTTGA AGAGGAGTGG ATGGGTTTAT TAGGGTACCT GTCCAAGATC 
TCTATTTGTA AAAAAAAGAA AGATTACTGT AAGAAGTACA AAAAACACGT GAGGAGCAGA 
TTCCAGTGTA TTAAAGACAG GAATGCCCGT CTGGGTGAGA GCGTGAACCT CAATAAACGC 
TACACGAGGC TGCGTCTTGT CAAGGAGCAC CAGAGCCAGC AGGAGAGGGA GCATGAGCTC 
CTGGCCATTG GCAGGACTTC AGCCAAGACG CTGGACAGCC CCATGAGTTC CCTGAATATG 
GAATTTCTGT TTGAGCCTGA CGACCAACAC TCGGAGCCTG TTCACACAGT GGTATTCCAG 
GGAGCAGCAG GCATTGGGAA AACCATACTG GCCAGGAAGA TCATGTTGGA CTGGGCATCA 
GAGAAAATTT ACCGGGATAG GTTTGACTAT TTGTTTTATA TCCACTGTCG GGAGGTGAGC 
CTTGGGACAC GGAGGAGCCT GGGGGACCTG ATCGTCAGCT GCTGCCCTGA CCCAAACCCA 
CCCATATATA AGATTGTGAG CAAGCCTTCC AGGATTCTCT TCCTCATGGA TGGCTTCGAT 
GAGCTGCAGG GCGCCTTTGA TGAGCACACG GAGGCTCTCT GCACAGACTG GCGGAAGGTG 
GAGCGAGGAG ACATTCTCCT GAGCAGCCTC ATCAGAAAGA GGCTACTTCC TGAGGCCTCT 
TTACTCATCA CCACAAGACC TGTGGCCCTG GAGAAACTGC AGCATTTGCT GGACCGTCCC 
CGTCATGTGG AGATCCTGGG TTTCTCAGAG GCCAAAAGAA AGGAATATTT CTTCAAGTAT 
TTCTCTGATG AGCAACAAGC CACAGAGGCC TTCAGGCTGA TTCAGGAGAA TGAGATCCTT 
TTTACAATGT GCTTTATCCC CTTGGTCTGC TGGATTGTGT GCACTGGGTT GAAGCAGCAG 
ATGGACAGTG GCAAGAGTCT TGCCCAGACA TCTAAGACCA CCACTGCAGT GTATGTCTTC 
TTCCTCTCCA GTTTGTTGCA ATCCTGTGGA GAGAACCAGA AGCACCAAGT CTCTGCCAAC 
ATCCGGGGTC TCTGCTCATT GGCTGCAGAT GGAATCTGGA ACCAGAAAAT CCTGTTTGAG 
GAGTGTGACC TTAGGAATCA TGGCCTTCAG AGGGCCGATG TGTCTGCTTT CTTGAGGATG 
AACCTATTCC AAAAGGAAGT GGACTGTGAG AAATTCTATA GCTTCATCCA CATGACTTTC 
CAGGAGTTCT TTGCTGCCAT GTACTATCTG CTGGAAGAGG AGGAACAGGG AGGGATGAGG 
AACCTGCCAT GGAGTAGTTC CAAGCTTCCC AACCGAGATG TGACCGTCCT TCTTGAAAAC 
TATGGCAAAT TTGAAAAGGG GTATCTGATT TTTGTTGTAC GTTTCCTCTT TGGCCTTGTA 
AACCAGGAGA GAACCTCCTA CTTGGAGAAG AAACTGAGTT GCAAGATCTC GCAGCAAATC 
AGGCTGGAGC TGCTGAAATG GATTAAAGAG AAAGCCGAGG CCAAGAATCT GCAGATCCAG 
CCCAGCCAGC TGGAGTTGTT CTACTGTTTG TACGAGATGC AAGAGGAGGA CTTTGTGCGA 
AAGGCCATGG GCCATTTCCC CAAAATTGAG ATCAGTCTCT CCACCAGAAT GGACCATGTG 
GTTTCTTCCT TTTGTATTGA GAACTGTCAC AGTGTGGAAT CACTTTCACT GAGGCTGCTT 
CATAACTCCC CGAAGGAAGA GGAGGATGAG GAGGAGGAAG AGGAAGGGCA ACACTCTGAG 
GTGGATCACT GTGTCCCCCT AGATCCTCAT GCTGCCTATA CTCACCGGTT GGTGAATTGT 
CATGTCACTG TCAGCCTTTG GCAGGGTCTC TTCTCAGTCC TGAGCACTAA CTCACTGAAC 
CTCACTGAAC TGAACCTCAG TGACAATGCT CTGGGGGACC CAGGGATAAA GGTGTTATGC 
GAAATGCTCC AGCATCCTGG CTGTAATATT CAGAGACTAT GGTTGGGGCA GTGTTGCCTT 
TCCCATCAGT GCTGCTTCAA CATCTCCTCG GTCCTGAGCA GCAACCAGAA GCTGGTGGAA 
TTGGATCTGA GCCACAACAC CCTGGGAGAC TTTGGAATTA GACTTCTTTG TGTGGGGCTG 
AGACACCTAT TCTGCAATCT GAAGAAGCTT TGGTTGATGA ATTCTGGCCT TACATCAGGT 
TGCTGTCCAG CTCTGTCCTC AGTGCTCAGC ACTAACCAGA AGCTCACACA CCTTTACCTA 
CGGGGCAATG CTCTTGGAGA CACAGGGGTG AAGCTACTCT GTGAGGGCCT CTTGCACCCC 
AACTGCAAGC TTCAGATATT AGAATTAGAT GGCTGTGGCC TCACCTCACA CTGTTGCTGG 
GATCTTTCTA CACTTCTGAC CTCCAATAAG AGCCTGCGGG AGCTGAGCCT GGGTAGTAAT 
GACCTGGATG ATCTAGGGGT CATGCTGCTC TGTGAAGTGC TAAAACAGCA GGGCTGCTGC 
CTAAAAAGCC TGAAGTTGTG TGAAATGTAT TTCAATTATG ATACAAAATG TGCATTAGAA 
ACACTTCAAG AAGAAAAGCC TGAGTTGACC ATTGTCTTTG AGCCTTTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012420190.1
CDS	23..2956
Translation	MSMASVRCKLARYLEDLEDADFKKFKMHLEDYPSQKGCSPLPRSQTEKADHMDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPEWDNAHVVCREESLEEEWMGLLGYLSKISICKKKKDYCKKYKKHVRSRFQCIKDRNARLGESVNLNKRYTRLRLVKEHQSQQEREHELLAIGRTSAKTLDSPMSSLNMEFLFEPDDQHSEPVHTVVFQGAAGIGKTILARKIMLDWASEKIYRDRFDYLFYIHCREVSLGTRRSLGDLIVSCCPDPNPPIYKIVSKPSRILFLMDGFDELQGAFDEHTEALCTDWRKVERGDILLSSLIRKRLLPEASLLITTRPVALEKLQHLLDRPRHVEILGFSEAKRKEYFFKYFSDEQQATEAFRLIQENEILFTMCFIPLVCWIVCTGLKQQMDSGKSLAQTSKTTTAVYVFFLSSLLQSCGENQKHQVSANIRGLCSLAADGIWNQKILFEECDLRNHGLQRADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEEQGGMRNLPWSSSKLPNRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIKEKAEAKNLQIQPSQLELFYCLYEMQEEDFVRKAMGHFPKIEISLSTRMDHVVSSFCIENCHSVESLSLRLLHNSPKEEEDEEEEEEGQHSEVDHCVPLDPHAAYTHRLVNCHVTVSLWQGLFSVLSTNSLNLTELNLSDNALGDPGIKVLCEMLQHPGCNIQRLWLGQCCLSHQCCFNISSVLSSNQKLVELDLSHNTLGDFGIRLLCVGLRHLFCNLKKLWLMNSGLTSGCCPALSSVLSTNQKLTHLYLRGNALGDTGVKLLCEGLLHPNCKLQILELDGCGLTSHCCWDLSTLLTSNKSLRELSLGSNDLDDLGVMLLCEVLKQQGCCLKSLKLCEMYFNYDTKCALETLQEEKPELTIVFEPFW

Target ORF information:

RefSeq Version	XM_012564736.1
Organism	Odobenus rosmarus divergens(Pacific walrus)
Definition	Odobenus rosmarus divergens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012564736.1

ORF Insert Sequence:

ATGAGCATGG CAAGTGTCCG TTGCAAGCTG GCCCGTTACC TGGAGGATCT GGAGGATGCA 
GACTTTAAGA AGTTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG GTGCAGCCCA 
CTCCCTCGGA GTCAGACAGA GAAAGCTGAC CACATGGATC TGGCCACTCT GATGATTGAC 
TTCAACGGTG AGGAGAAGGC ATGGGCCATG GCAGTGTGGA TTTTTGCTGC AATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAACCAGAGT GGGATAATGC ACATGTGGTA 
TGCCGGGAAG AAAGCCTTGA AGAGGAGTGG ATGGGTTTAT TAGGGTACCT GTCCAAGATC 
TCTATTTGTA AAAAAAAGAA AGATTACTGT AAGAAGTACA AAAAACACGT GAGGAGCAGA 
TTCCAGTGTA TTAAAGACAG GAATGCCCGT CTGGGTGAGA GCGTGAACCT CAATAAACGC 
TACACGAGGC TGCGTCTTGT CAAGGAGCAC CAGAGCCAGC AGGAGAGGGA GCATGAGCTC 
CTGGCCATTG GCAGGACTTC AGCCAAGACG CTGGACAGCC CCATGAGTTC CCTGAATATG 
GAATTTCTGT TTGAGCCTGA CGACCAACAC TCGGAGCCTG TTCACACAGT GGTATTCCAG 
GGAGCAGCAG GCATTGGGAA AACCATACTG GCCAGGAAGA TCATGTTGGA CTGGGCATCA 
GAGAAAATTT ACCGGGATAG GTTTGACTAT TTGTTTTATA TCCACTGTCG GGAGGTGAGC 
CTTGGGACAC GGAGGAGCCT GGGGGACCTG ATCGTCAGCT GCTGCCCTGA CCCAAACCCA 
CCCATATATA AGATTGTGAG CAAGCCTTCC AGGATTCTCT TCCTCATGGA TGGCTTCGAT 
GAGCTGCAGG GCGCCTTTGA TGAGCACACG GAGGCTCTCT GCACAGACTG GCGGAAGGTG 
GAGCGAGGAG ACATTCTCCT GAGCAGCCTC ATCAGAAAGA GGCTACTTCC TGAGGCCTCT 
TTACTCATCA CCACAAGACC TGTGGCCCTG GAGAAACTGC AGCATTTGCT GGACCGTCCC 
CGTCATGTGG AGATCCTGGG TTTCTCAGAG GCCAAAAGAA AGGAATATTT CTTCAAGTAT 
TTCTCTGATG AGCAACAAGC CACAGAGGCC TTCAGGCTGA TTCAGGAGAA TGAGATCCTT 
TTTACAATGT GCTTTATCCC CTTGGTCTGC TGGATTGTGT GCACTGGGTT GAAGCAGCAG 
ATGGACAGTG GCAAGAGTCT TGCCCAGACA TCTAAGACCA CCACTGCAGT GTATGTCTTC 
TTCCTCTCCA GTTTGTTGCA ATCCTGTGGA GAGAACCAGA AGCACCAAGT CTCTGCCAAC 
ATCCGGGGTC TCTGCTCATT GGCTGCAGAT GGAATCTGGA ACCAGAAAAT CCTGTTTGAG 
GAGTGTGACC TTAGGAATCA TGGCCTTCAG AGGGCCGATG TGTCTGCTTT CTTGAGGATG 
AACCTATTCC AAAAGGAAGT GGACTGTGAG AAATTCTATA GCTTCATCCA CATGACTTTC 
CAGGAGTTCT TTGCTGCCAT GTACTATCTG CTGGAAGAGG AGGAACAGGG AGGGATGAGG 
AACCTGCCAT GGAGTAGTTC CAAGCTTCCC AACCGAGATG TGACCGTCCT TCTTGAAAAC 
TATGGCAAAT TTGAAAAGGG GTATCTGATT TTTGTTGTAC GTTTCCTCTT TGGCCTTGTA 
AACCAGGAGA GAACCTCCTA CTTGGAGAAG AAACTGAGTT GCAAGATCTC GCAGCAAATC 
AGGCTGGAGC TGCTGAAATG GATTAAAGAG AAAGCCGAGG CCAAGAATCT GCAGATCCAG 
CCCAGCCAGC TGGAGTTGTT CTACTGTTTG TACGAGATGC AAGAGGAGGA CTTTGTGCGA 
AAGGCCATGG GCCATTTCCC CAAAATTGAG ATCAGTCTCT CCACCAGAAT GGACCATGTG 
GTTTCTTCCT TTTGTATTGA GAACTGTCAC AGTGTGGAAT CACTTTCACT GAGGCTGCTT 
CATAACTCCC CGAAGGAAGA GGAGGATGAG GAGGAGGAAG AGGAAGGGCA ACACTCTGAG 
GTGGATCACT GTGTCCCCCT AGATCCTCAT GCTGCCTATA CTCACCGGTT GGTGAATTGT 
CATGTCACTG TCAGCCTTTG GCAGGGTCTC TTCTCAGTCC TGAGCACTAA CTCACTGAAC 
CTCACTGAAC TGAACCTCAG TGACAATGCT CTGGGGGACC CAGGGATAAA GGTGTTATGC 
GAAATGCTCC AGCATCCTGG CTGTAATATT CAGAGACTAT GGTTGGGGCA GTGTTGCCTT 
TCCCATCAGT GCTGCTTCAA CATCTCCTCG GTCCTGAGCA GCAACCAGAA GCTGGTGGAA 
TTGGATCTGA GCCACAACAC CCTGGGAGAC TTTGGAATTA GACTTCTTTG TGTGGGGCTG 
AGACACCTAT TCTGCAATCT GAAGAAGCTT TGGTTGATGA ATTCTGGCCT TACATCAGGT 
TGCTGTCCAG CTCTGTCCTC AGTGCTCAGC ACTAACCAGA AGCTCACACA CCTTTACCTA 
CGGGGCAATG CTCTTGGAGA CACAGGGGTG AAGCTACTCT GTGAGGGCCT CTTGCACCCC 
AACTGCAAGC TTCAGATATT AGAATTAGAT GGCTGTGGCC TCACCTCACA CTGTTGCTGG 
GATCTTTCTA CACTTCTGAC CTCCAATAAG AGCCTGCGGG AGCTGAGCCT GGGTAGTAAT 
GACCTGGATG ATCTAGGGGT CATGCTGCTC TGTGAAGTGC TAAAACAGCA GGGCTGCTGC 
CTAAAAAGCC TGAAGTTGTG TGAAATGTAT TTCAATTATG ATACAAAATG TGCATTAGAA 
ACACTTCAAG AAGAAAAGCC TGAGTTGACC ATTGTCTTTG AGCCTTTTTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NLRP3 ( XM_004407072.2 ) cDNA ORF clone, Odobenus rosmarus divergens(Pacific walrus) -> XP_004407129.1 Odobenus rosmarus divergens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OOd60822
Clone ID Related Accession (Same CDS sequence)	XM_004407072.2
Accession Version	XM_004407072.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-25
Organism	Odobenus rosmarus divergens(Pacific walrus)
Product	NACHT, LRR and PYD domains-containing protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004450584.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 26, 2015 this sequence version replaced XM_004407072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Odobenus rosmarus divergens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCATGG CAAGTGTCCG TTGCAAGCTG GCCCGTTACC TGGAGGATCT GGAGGATGCA 
GACTTTAAGA AGTTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG GTGCAGCCCA 
CTCCCTCGGA GTCAGACAGA GAAAGCTGAC CACATGGATC TGGCCACTCT GATGATTGAC 
TTCAACGGTG AGGAGAAGGC ATGGGCCATG GCAGTGTGGA TTTTTGCTGC AATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAACCAGAGT GGGATAATGC ACATGTGGTA 
TGCCGGGAAG AAAGCCTTGA AGAGGAGTGG ATGGGTTTAT TAGGGTACCT GTCCAAGATC 
TCTATTTGTA AAAAAAAGAA AGATTACTGT AAGAAGTACA AAAAACACGT GAGGAGCAGA 
TTCCAGTGTA TTAAAGACAG GAATGCCCGT CTGGGTGAGA GCGTGAACCT CAATAAACGC 
TACACGAGGC TGCGTCTTGT CAAGGAGCAC CAGAGCCAGC AGGAGAGGGA GCATGAGCTC 
CTGGCCATTG GCAGGACTTC AGCCAAGACG CTGGACAGCC CCATGAGTTC CCTGAATATG 
GAATTTCTGT TTGAGCCTGA CGACCAACAC TCGGAGCCTG TTCACACAGT GGTATTCCAG 
GGAGCAGCAG GCATTGGGAA AACCATACTG GCCAGGAAGA TCATGTTGGA CTGGGCATCA 
GAGAAAATTT ACCGGGATAG GTTTGACTAT TTGTTTTATA TCCACTGTCG GGAGGTGAGC 
CTTGGGACAC GGAGGAGCCT GGGGGACCTG ATCGTCAGCT GCTGCCCTGA CCCAAACCCA 
CCCATATATA AGATTGTGAG CAAGCCTTCC AGGATTCTCT TCCTCATGGA TGGCTTCGAT 
GAGCTGCAGG GCGCCTTTGA TGAGCACACG GAGGCTCTCT GCACAGACTG GCGGAAGGTG 
GAGCGAGGAG ACATTCTCCT GAGCAGCCTC ATCAGAAAGA GGCTACTTCC TGAGGCCTCT 
TTACTCATCA CCACAAGACC TGTGGCCCTG GAGAAACTGC AGCATTTGCT GGACCGTCCC 
CGTCATGTGG AGATCCTGGG TTTCTCAGAG GCCAAAAGAA AGGAATATTT CTTCAAGTAT 
TTCTCTGATG AGCAACAAGC CACAGAGGCC TTCAGGCTGA TTCAGGAGAA TGAGATCCTT 
TTTACAATGT GCTTTATCCC CTTGGTCTGC TGGATTGTGT GCACTGGGTT GAAGCAGCAG 
ATGGACAGTG GCAAGAGTCT TGCCCAGACA TCTAAGACCA CCACTGCAGT GTATGTCTTC 
TTCCTCTCCA GTTTGTTGCA ATCCTGTGGA GAGAACCAGA AGCACCAAGT CTCTGCCAAC 
ATCCGGGGTC TCTGCTCATT GGCTGCAGAT GGAATCTGGA ACCAGAAAAT CCTGTTTGAG 
GAGTGTGACC TTAGGAATCA TGGCCTTCAG AGGGCCGATG TGTCTGCTTT CTTGAGGATG 
AACCTATTCC AAAAGGAAGT GGACTGTGAG AAATTCTATA GCTTCATCCA CATGACTTTC 
CAGGAGTTCT TTGCTGCCAT GTACTATCTG CTGGAAGAGG AGGAACAGGG AGGGATGAGG 
AACCTGCCAT GGAGTAGTTC CAAGCTTCCC AACCGAGATG TGACCGTCCT TCTTGAAAAC 
TATGGCAAAT TTGAAAAGGG GTATCTGATT TTTGTTGTAC GTTTCCTCTT TGGCCTTGTA 
AACCAGGAGA GAACCTCCTA CTTGGAGAAG AAACTGAGTT GCAAGATCTC GCAGCAAATC 
AGGCTGGAGC TGCTGAAATG GATTAAAGAG AAAGCCGAGG CCAAGAATCT GCAGATCCAG 
CCCAGCCAGC TGGAGTTGTT CTACTGTTTG TACGAGATGC AAGAGGAGGA CTTTGTGCGA 
AAGGCCATGG GCCATTTCCC CAAAATTGAG ATCAGTCTCT CCACCAGAAT GGACCATGTG 
GTTTCTTCCT TTTGTATTGA GAACTGTCAC AGTGTGGAAT CACTTTCACT GAGGCTGCTT 
CATAACTCCC CGAAGGAAGA GGAGGATGAG GAGGAGGAAG AGGAAGGGCA ACACTCTGAG 
GTGGATCACT GTGTCCCCCT AGATCCTCAT GCTGCCTATA CTCACCGGTT GGTGAATTGT 
CATGTCACTG TCAGCCTTTG GCAGGGTCTC TTCTCAGTCC TGAGCACTAA CTCACTGAAC 
CTCACTGAAC TGAACCTCAG TGACAATGCT CTGGGGGACC CAGGGATAAA GGTGTTATGC 
GAAATGCTCC AGCATCCTGG CTGTAATATT CAGAGACTAT GGTTGGGGCA GTGTTGCCTT 
TCCCATCAGT GCTGCTTCAA CATCTCCTCG GTCCTGAGCA GCAACCAGAA GCTGGTGGAA 
TTGGATCTGA GCCACAACAC CCTGGGAGAC TTTGGAATTA GACTTCTTTG TGTGGGGCTG 
AGACACCTAT TCTGCAATCT GAAGAAGCTT TGGTTGGTCA ATTGCTGTCT CACATCAGCA 
TGTTGTGAGG ACCTTGCATC CGTCCTGAGC ACCAACCAGT CCCTGACCAG ACTCTACTTG 
GGGGAAAATG CCCTGGGAGA TGCAGGAGTT GGAATTTTAT GTGAAAAAGC AAAGCATCCA 
CAATGTAAGC TGCAGAAACT GGGGTTGATG AATTCTGGCC TTACATCAGG TTGCTGTCCA 
GCTCTGTCCT CAGTGCTCAG CACTAACCAG AAGCTCACAC ACCTTTACCT ACGGGGCAAT 
GCTCTTGGAG ACACAGGGGT GAAGCTACTC TGTGAGGGCC TCTTGCACCC CAACTGCAAG 
CTTCAGATAT TAGAATTAGA TGGCTGTGGC CTCACCTCAC ACTGTTGCTG GGATCTTTCT 
ACACTTCTGA CCTCCAATAA GAGCCTGCGG GAGCTGAGCC TGGGTAGTAA TGACCTGGAT 
GATCTAGGGG TCATGCTGCT CTGTGAAGTG CTAAAACAGC AGGGCTGCTG CCTAAAAAGC 
CTGAAGTTGT GTGAAATGTA TTTCAATTAT GATACAAAAT GTGCATTAGA AACACTTCAA 
GAAGAAAAGC CTGAGTTGAC CATTGTCTTT GAGCCTTTTT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004407129.1
CDS	83..3187
Translation	MSMASVRCKLARYLEDLEDADFKKFKMHLEDYPSQKGCSPLPRSQTEKADHMDLATLMIDFNGEEKAWAMAVWIFAAINRRDLYEKAKRDEPEWDNAHVVCREESLEEEWMGLLGYLSKISICKKKKDYCKKYKKHVRSRFQCIKDRNARLGESVNLNKRYTRLRLVKEHQSQQEREHELLAIGRTSAKTLDSPMSSLNMEFLFEPDDQHSEPVHTVVFQGAAGIGKTILARKIMLDWASEKIYRDRFDYLFYIHCREVSLGTRRSLGDLIVSCCPDPNPPIYKIVSKPSRILFLMDGFDELQGAFDEHTEALCTDWRKVERGDILLSSLIRKRLLPEASLLITTRPVALEKLQHLLDRPRHVEILGFSEAKRKEYFFKYFSDEQQATEAFRLIQENEILFTMCFIPLVCWIVCTGLKQQMDSGKSLAQTSKTTTAVYVFFLSSLLQSCGENQKHQVSANIRGLCSLAADGIWNQKILFEECDLRNHGLQRADVSAFLRMNLFQKEVDCEKFYSFIHMTFQEFFAAMYYLLEEEEQGGMRNLPWSSSKLPNRDVTVLLENYGKFEKGYLIFVVRFLFGLVNQERTSYLEKKLSCKISQQIRLELLKWIKEKAEAKNLQIQPSQLELFYCLYEMQEEDFVRKAMGHFPKIEISLSTRMDHVVSSFCIENCHSVESLSLRLLHNSPKEEEDEEEEEEGQHSEVDHCVPLDPHAAYTHRLVNCHVTVSLWQGLFSVLSTNSLNLTELNLSDNALGDPGIKVLCEMLQHPGCNIQRLWLGQCCLSHQCCFNISSVLSSNQKLVELDLSHNTLGDFGIRLLCVGLRHLFCNLKKLWLVNCCLTSACCEDLASVLSTNQSLTRLYLGENALGDAGVGILCEKAKHPQCKLQKLGLMNSGLTSGCCPALSSVLSTNQKLTHLYLRGNALGDTGVKLLCEGLLHPNCKLQILELDGCGLTSHCCWDLSTLLTSNKSLRELSLGSNDLDDLGVMLLCEVLKQQGCCLKSLKLCEMYFNYDTKCALETLQEEKPELTIVFEPFW

Target ORF information:

RefSeq Version	XM_004407072.2
Organism	Odobenus rosmarus divergens(Pacific walrus)
Definition	Odobenus rosmarus divergens NLR family, pyrin domain containing 3 (NLRP3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004407072.2

ORF Insert Sequence:

ATGAGCATGG CAAGTGTCCG TTGCAAGCTG GCCCGTTACC TGGAGGATCT GGAGGATGCA 
GACTTTAAGA AGTTCAAGAT GCACTTAGAA GACTATCCTT CTCAGAAGGG GTGCAGCCCA 
CTCCCTCGGA GTCAGACAGA GAAAGCTGAC CACATGGATC TGGCCACTCT GATGATTGAC 
TTCAACGGTG AGGAGAAGGC ATGGGCCATG GCAGTGTGGA TTTTTGCTGC AATCAACAGG 
AGAGACCTTT ATGAGAAAGC TAAGAGGGAT GAACCAGAGT GGGATAATGC ACATGTGGTA 
TGCCGGGAAG AAAGCCTTGA AGAGGAGTGG ATGGGTTTAT TAGGGTACCT GTCCAAGATC 
TCTATTTGTA AAAAAAAGAA AGATTACTGT AAGAAGTACA AAAAACACGT GAGGAGCAGA 
TTCCAGTGTA TTAAAGACAG GAATGCCCGT CTGGGTGAGA GCGTGAACCT CAATAAACGC 
TACACGAGGC TGCGTCTTGT CAAGGAGCAC CAGAGCCAGC AGGAGAGGGA GCATGAGCTC 
CTGGCCATTG GCAGGACTTC AGCCAAGACG CTGGACAGCC CCATGAGTTC CCTGAATATG 
GAATTTCTGT TTGAGCCTGA CGACCAACAC TCGGAGCCTG TTCACACAGT GGTATTCCAG 
GGAGCAGCAG GCATTGGGAA AACCATACTG GCCAGGAAGA TCATGTTGGA CTGGGCATCA 
GAGAAAATTT ACCGGGATAG GTTTGACTAT TTGTTTTATA TCCACTGTCG GGAGGTGAGC 
CTTGGGACAC GGAGGAGCCT GGGGGACCTG ATCGTCAGCT GCTGCCCTGA CCCAAACCCA 
CCCATATATA AGATTGTGAG CAAGCCTTCC AGGATTCTCT TCCTCATGGA TGGCTTCGAT 
GAGCTGCAGG GCGCCTTTGA TGAGCACACG GAGGCTCTCT GCACAGACTG GCGGAAGGTG 
GAGCGAGGAG ACATTCTCCT GAGCAGCCTC ATCAGAAAGA GGCTACTTCC TGAGGCCTCT 
TTACTCATCA CCACAAGACC TGTGGCCCTG GAGAAACTGC AGCATTTGCT GGACCGTCCC 
CGTCATGTGG AGATCCTGGG TTTCTCAGAG GCCAAAAGAA AGGAATATTT CTTCAAGTAT 
TTCTCTGATG AGCAACAAGC CACAGAGGCC TTCAGGCTGA TTCAGGAGAA TGAGATCCTT 
TTTACAATGT GCTTTATCCC CTTGGTCTGC TGGATTGTGT GCACTGGGTT GAAGCAGCAG 
ATGGACAGTG GCAAGAGTCT TGCCCAGACA TCTAAGACCA CCACTGCAGT GTATGTCTTC 
TTCCTCTCCA GTTTGTTGCA ATCCTGTGGA GAGAACCAGA AGCACCAAGT CTCTGCCAAC 
ATCCGGGGTC TCTGCTCATT GGCTGCAGAT GGAATCTGGA ACCAGAAAAT CCTGTTTGAG 
GAGTGTGACC TTAGGAATCA TGGCCTTCAG AGGGCCGATG TGTCTGCTTT CTTGAGGATG 
AACCTATTCC AAAAGGAAGT GGACTGTGAG AAATTCTATA GCTTCATCCA CATGACTTTC 
CAGGAGTTCT TTGCTGCCAT GTACTATCTG CTGGAAGAGG AGGAACAGGG AGGGATGAGG 
AACCTGCCAT GGAGTAGTTC CAAGCTTCCC AACCGAGATG TGACCGTCCT TCTTGAAAAC 
TATGGCAAAT TTGAAAAGGG GTATCTGATT TTTGTTGTAC GTTTCCTCTT TGGCCTTGTA 
AACCAGGAGA GAACCTCCTA CTTGGAGAAG AAACTGAGTT GCAAGATCTC GCAGCAAATC 
AGGCTGGAGC TGCTGAAATG GATTAAAGAG AAAGCCGAGG CCAAGAATCT GCAGATCCAG 
CCCAGCCAGC TGGAGTTGTT CTACTGTTTG TACGAGATGC AAGAGGAGGA CTTTGTGCGA 
AAGGCCATGG GCCATTTCCC CAAAATTGAG ATCAGTCTCT CCACCAGAAT GGACCATGTG 
GTTTCTTCCT TTTGTATTGA GAACTGTCAC AGTGTGGAAT CACTTTCACT GAGGCTGCTT 
CATAACTCCC CGAAGGAAGA GGAGGATGAG GAGGAGGAAG AGGAAGGGCA ACACTCTGAG 
GTGGATCACT GTGTCCCCCT AGATCCTCAT GCTGCCTATA CTCACCGGTT GGTGAATTGT 
CATGTCACTG TCAGCCTTTG GCAGGGTCTC TTCTCAGTCC TGAGCACTAA CTCACTGAAC 
CTCACTGAAC TGAACCTCAG TGACAATGCT CTGGGGGACC CAGGGATAAA GGTGTTATGC 
GAAATGCTCC AGCATCCTGG CTGTAATATT CAGAGACTAT GGTTGGGGCA GTGTTGCCTT 
TCCCATCAGT GCTGCTTCAA CATCTCCTCG GTCCTGAGCA GCAACCAGAA GCTGGTGGAA 
TTGGATCTGA GCCACAACAC CCTGGGAGAC TTTGGAATTA GACTTCTTTG TGTGGGGCTG 
AGACACCTAT TCTGCAATCT GAAGAAGCTT TGGTTGGTCA ATTGCTGTCT CACATCAGCA 
TGTTGTGAGG ACCTTGCATC CGTCCTGAGC ACCAACCAGT CCCTGACCAG ACTCTACTTG 
GGGGAAAATG CCCTGGGAGA TGCAGGAGTT GGAATTTTAT GTGAAAAAGC AAAGCATCCA 
CAATGTAAGC TGCAGAAACT GGGGTTGATG AATTCTGGCC TTACATCAGG TTGCTGTCCA 
GCTCTGTCCT CAGTGCTCAG CACTAACCAG AAGCTCACAC ACCTTTACCT ACGGGGCAAT 
GCTCTTGGAG ACACAGGGGT GAAGCTACTC TGTGAGGGCC TCTTGCACCC CAACTGCAAG 
CTTCAGATAT TAGAATTAGA TGGCTGTGGC CTCACCTCAC ACTGTTGCTG GGATCTTTCT 
ACACTTCTGA CCTCCAATAA GAGCCTGCGG GAGCTGAGCC TGGGTAGTAA TGACCTGGAT 
GATCTAGGGG TCATGCTGCT CTGTGAAGTG CTAAAACAGC AGGGCTGCTG CCTAAAAAGC 
CTGAAGTTGT GTGAAATGTA TTTCAATTAT GATACAAAAT GTGCATTAGA AACACTTCAA 
GAAGAAAAGC CTGAGTTGAC CATTGTCTTT GAGCCTTTTT GGTAG