EDEM1 cDNA ORF clone, Numida meleagris(helmeted guineafowl)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDEM1
Entrez Gene ID	110404705
Full Name	ER degradation enhancing alpha-mannosidase like protein 1
Gene Type	protein-coding
Organism	Numida meleagris(helmeted guineafowl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021409266.1	XP_021264941.1	ER degradation-enhancing alpha-mannosidase-like protein 1

Pathways from BioSystems
Gene Ontology

The following EDEM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDEM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONu06586	XM_021409266.1 Latest version!	Numida meleagris ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDEM1 ( XM_021409266.1 ) cDNA ORF clone, Numida meleagris(helmeted guineafowl) -> XP_021264941.1 Numida meleagris ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X1, mRNA.

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CloneID	ONu06586
Clone ID Related Accession (Same CDS sequence)	XM_021409266.1
Accession Version	XM_021409266.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-06
Organism	Numida meleagris(helmeted guineafowl)
Product	ER degradation-enhancing alpha-mannosidase-like protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_034419.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Numida meleagris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAATGGC GCTCGCTGGT GCTGGGGCTG CTGCTGCTGA GGCTGGGGCT GCACGCGCTG 
CTGGCGCTGC TCCCCGCGCT GGCTCCGGGC CTCTGCCTGC GCCTCCGCCT CCCGTTCCCC 
TTCCCTCCGC GCTCCGCCGC CCTCGGTCCT CCCGGCGCCG CGCGGCCGCT GCCCGGCGGG 
GGCTCCTCGC ACTGGGGTTC GCTGCGGGGC GGGGGCGGCG CGGCGGGGCG CGATGAGTAC 
GAGACGCGCT ACAGCGGCGC GTTCCCCCCG CAGCTGCGGG CCCGGCTGCG CGACGCCGCC 
CGCGGCATGT TCGCCTTCGG CTACGACAGC TACATGCAGC ACGCCTTCCC CAGCGACGAG 
CTCGACCCCC TGCACTGCTG CGGCCGCGGG CCCGACCGCG ACGACCCTTC CAATCTGAAC 
ATCAACGATG TCTTAGGGAA CTACTCGCTA ACGCTGATAG ATGCACTGGA TACCCTAGCG 
GTAATGGGCA ATTCCTCCGA ATTCCAGAAA GCGGTGAAGT TGGTGATTGA CACAGTTTCA 
TTTGACAAAG ACTCAACAGT CCAAGTGTTT GAGGCAACCA TCAGGGTTTT GGGAAGCCTG 
CTTTCTGCCC ACATCATAAT TACGGATGCC AAACAGCCTT TTGGGGATAT GACCATTAAG 
GATTATGACA ACGAACTGCT GCACATGGCT CATGACCTAG CAGTGAGACT ACTCCCAGCA 
TTTGAGAACA CCAAAACTGG AATTCCGTAT CCTCGGGTCA ACCTGAAGAA GGGGGTTCCT 
CCAAACAGCA ATAATGAAAC TTGCACTGCA GGAGCCGGTT CCTTGTTGGT GGAGTTTGGT 
ATTCTCAGCA GGCTGCTTGG CGATTCCACA TTTGAATGGG TGGCCAGGAG AGCTGTCAAA 
GCACTCTGGA GTCTCAGGAG CAATAACACT GGACTGCTAG GAAATGTTGT GAATATTCAA 
ACTGGCCATT GGGTTGGAAA GCAAAGTGGC TTGGGAGCAG GATCGGATTC GTTTTATGAA 
TACCTCCTGA AGTCTTACAT CCTCTTTGGA GAGAAGGAAG ACTTGGAGAT GTTCATTGAT 
GCTTATCAGA ACATTCAGAA CCACTTACGA AGGGGTCGAG AAGCTTGCAA TGAAGGTGAA 
GGTGACCCGC CTTTGTATGT TAATGTGAAC ATGTTCACTG GACAGCTGAT GAACACGTGG 
ATTGACTCTT TGCAAGCCTT TTTTCCTGGA CTACAGGTGT TGATAGGAGA CGTAGAAGAT 
GCTATTTGTC TCCATGCTTT TTATTATGCC ATATGGAAAC GTTATGGAGC TCTCCCAGAA 
AGGTACAACT GGCAATTGCA AGCACCGGAT GTTCCCTTTT ATCCACTGAG GCCCGAGTTA 
GTGGAATCCA CGTATCTTCT GTACCAGGCC ACAAAGAACC CATTTTACCT TCATGTGGGA 
ATGGATATTC TGCAGAGTTT GGAAAAATAC ACAAAAGCGA AGTGTGGCTA TGCTACGTTA 
CACCATGTTG TGGAGAAGAC AAAAGAAGAT CGAATGGAGA GCTTTTTTCT CAGCGAAACA 
TGTAAATATT TGTACCTGTT GTTTGATGAA GACAATCCAG TGCATAAGTC TGGAAATAAA 
TATATGTTTA CTACTGAGGG ACATATTGTG TCTGTGGATG AACGTTTTCG CAACTCGCTG 
TGGCAGGACA TCCTCCCCGG AGAAGAGGAC AGCACAGAAA AAGTTAAACC TAACGAATTA 
AAGGCTGCCA ACTTCAGTTC TAATTGTAAC AGAGTTCCTG ATGAGAGGAG ATACCTGCTG 
CCTTTGAAGA GCAACTACAT GAGACAGATC GATCGGATGG TGGGCCTGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021264941.1
CDS	70..1923
Translation	MQWRSLVLGLLLLRLGLHALLALLPALAPGLCLRLRLPFPFPPRSAALGPPGAARPLPGGGSSHWGSLRGGGGAAGRDEYETRYSGAFPPQLRARLRDAARGMFAFGYDSYMQHAFPSDELDPLHCCGRGPDRDDPSNLNINDVLGNYSLTLIDALDTLAVMGNSSEFQKAVKLVIDTVSFDKDSTVQVFEATIRVLGSLLSAHIIITDAKQPFGDMTIKDYDNELLHMAHDLAVRLLPAFENTKTGIPYPRVNLKKGVPPNSNNETCTAGAGSLLVEFGILSRLLGDSTFEWVARRAVKALWSLRSNNTGLLGNVVNIQTGHWVGKQSGLGAGSDSFYEYLLKSYILFGEKEDLEMFIDAYQNIQNHLRRGREACNEGEGDPPLYVNVNMFTGQLMNTWIDSLQAFFPGLQVLIGDVEDAICLHAFYYAIWKRYGALPERYNWQLQAPDVPFYPLRPELVESTYLLYQATKNPFYLHVGMDILQSLEKYTKAKCGYATLHHVVEKTKEDRMESFFLSETCKYLYLLFDEDNPVHKSGNKYMFTTEGHIVSVDERFRNSLWQDILPGEEDSTEKVKPNELKAANFSSNCNRVPDERRYLLPLKSNYMRQIDRMVGLI

Target ORF information:

RefSeq Version	XM_021409266.1
Organism	Numida meleagris(helmeted guineafowl)
Definition	Numida meleagris ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021409266.1

ORF Insert Sequence:

ATGCAATGGC GCTCGCTGGT GCTGGGGCTG CTGCTGCTGA GGCTGGGGCT GCACGCGCTG 
CTGGCGCTGC TCCCCGCGCT GGCTCCGGGC CTCTGCCTGC GCCTCCGCCT CCCGTTCCCC 
TTCCCTCCGC GCTCCGCCGC CCTCGGTCCT CCCGGCGCCG CGCGGCCGCT GCCCGGCGGG 
GGCTCCTCGC ACTGGGGTTC GCTGCGGGGC GGGGGCGGCG CGGCGGGGCG CGATGAGTAC 
GAGACGCGCT ACAGCGGCGC GTTCCCCCCG CAGCTGCGGG CCCGGCTGCG CGACGCCGCC 
CGCGGCATGT TCGCCTTCGG CTACGACAGC TACATGCAGC ACGCCTTCCC CAGCGACGAG 
CTCGACCCCC TGCACTGCTG CGGCCGCGGG CCCGACCGCG ACGACCCTTC CAATCTGAAC 
ATCAACGATG TCTTAGGGAA CTACTCGCTA ACGCTGATAG ATGCACTGGA TACCCTAGCG 
GTAATGGGCA ATTCCTCCGA ATTCCAGAAA GCGGTGAAGT TGGTGATTGA CACAGTTTCA 
TTTGACAAAG ACTCAACAGT CCAAGTGTTT GAGGCAACCA TCAGGGTTTT GGGAAGCCTG 
CTTTCTGCCC ACATCATAAT TACGGATGCC AAACAGCCTT TTGGGGATAT GACCATTAAG 
GATTATGACA ACGAACTGCT GCACATGGCT CATGACCTAG CAGTGAGACT ACTCCCAGCA 
TTTGAGAACA CCAAAACTGG AATTCCGTAT CCTCGGGTCA ACCTGAAGAA GGGGGTTCCT 
CCAAACAGCA ATAATGAAAC TTGCACTGCA GGAGCCGGTT CCTTGTTGGT GGAGTTTGGT 
ATTCTCAGCA GGCTGCTTGG CGATTCCACA TTTGAATGGG TGGCCAGGAG AGCTGTCAAA 
GCACTCTGGA GTCTCAGGAG CAATAACACT GGACTGCTAG GAAATGTTGT GAATATTCAA 
ACTGGCCATT GGGTTGGAAA GCAAAGTGGC TTGGGAGCAG GATCGGATTC GTTTTATGAA 
TACCTCCTGA AGTCTTACAT CCTCTTTGGA GAGAAGGAAG ACTTGGAGAT GTTCATTGAT 
GCTTATCAGA ACATTCAGAA CCACTTACGA AGGGGTCGAG AAGCTTGCAA TGAAGGTGAA 
GGTGACCCGC CTTTGTATGT TAATGTGAAC ATGTTCACTG GACAGCTGAT GAACACGTGG 
ATTGACTCTT TGCAAGCCTT TTTTCCTGGA CTACAGGTGT TGATAGGAGA CGTAGAAGAT 
GCTATTTGTC TCCATGCTTT TTATTATGCC ATATGGAAAC GTTATGGAGC TCTCCCAGAA 
AGGTACAACT GGCAATTGCA AGCACCGGAT GTTCCCTTTT ATCCACTGAG GCCCGAGTTA 
GTGGAATCCA CGTATCTTCT GTACCAGGCC ACAAAGAACC CATTTTACCT TCATGTGGGA 
ATGGATATTC TGCAGAGTTT GGAAAAATAC ACAAAAGCGA AGTGTGGCTA TGCTACGTTA 
CACCATGTTG TGGAGAAGAC AAAAGAAGAT CGAATGGAGA GCTTTTTTCT CAGCGAAACA 
TGTAAATATT TGTACCTGTT GTTTGATGAA GACAATCCAG TGCATAAGTC TGGAAATAAA 
TATATGTTTA CTACTGAGGG ACATATTGTG TCTGTGGATG AACGTTTTCG CAACTCGCTG 
TGGCAGGACA TCCTCCCCGG AGAAGAGGAC AGCACAGAAA AAGTTAAACC TAACGAATTA 
AAGGCTGCCA ACTTCAGTTC TAATTGTAAC AGAGTTCCTG ATGAGAGGAG ATACCTGCTG 
CCTTTGAAGA GCAACTACAT GAGACAGATC GATCGGATGG TGGGCCTGAT CTGA