RASGRF1 cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASGRF1
Entrez Gene ID	100597935
Full Name	Ras protein specific guanine nucleotide releasing factor 1
Gene Type	protein-coding
Organism	Nomascus leucogenys(northern white-cheeked gibbon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003275431.3	XP_003275479.1	LOW QUALITY PROTEIN: ras-specific guanine nucleotide-releasing factor 1
XM_030814881.1	XP_030670741.1	ras-specific guanine nucleotide-releasing factor 1 isoform X2
XM_030814883.1	XP_030670743.1	ras-specific guanine nucleotide-releasing factor 1 isoform X4
XM_030814880.1	XP_030670740.1	ras-specific guanine nucleotide-releasing factor 1 isoform X1
XM_030814884.1	XP_030670744.1	ras-specific guanine nucleotide-releasing factor 1 isoform X5
XM_030814885.1	XP_030670745.1	ras-specific guanine nucleotide-releasing factor 1 isoform X6
XM_030814882.1	XP_030670742.1	ras-specific guanine nucleotide-releasing factor 1 isoform X3

Pathways from BioSystems
Gene Ontology

The following RASGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ONo176982	XM_030814880.1 Latest version!	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONo176983	XM_030814881.1 Latest version!	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONo176984	XM_030814882.1 Latest version!	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONo176985	XM_030814883.1 Latest version!	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONo176986	XM_030814884.1 Latest version!	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONo176987	XM_030814885.1 Latest version!	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ONo65853	XM_003275431.3 Latest version!	Nomascus leucogenys Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASGRF1 ( XM_030814880.1 ) cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon) -> XP_030670740.1 Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ONo176982
Clone ID Related Accession (Same CDS sequence)	XM_030814880.1
Accession Version	XM_030814880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nomascus leucogenys Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAAACA TTTCAAAAAC CAGAAAAAAC AAAACACAGG 
CTCCAGGACC ACTGCGTTTG TTTGAACAAT CCCCAGAGAC AAGAGGGATT TAGCAGGTGT 
CCTACTAAAC GCAGCTCTCC AAGCCCTGGG AGGACCAGGG AGGGGACCAG GGAAGCAGAA 
ATGAGCAGGT CTGACGCCTC CTTATATTGT GATGATGTTG ACATTCGCTT CAGCAAAACC 
ATGAACTCCT GCAAAGTGCT GCAGATCCGC TACGCCAGTG TGGAGCGGCT GCTGGAGAGG 
CTGACGGACC TGCGCTTCCT GAGCATCGAC TTCCTCAACA CCTTCCTGCA CTCCTACCGC 
GTCTTCACCA CCGCCATCGT GGTCCTGGAC AAGCTCATTA CCATCTACAA GAAGCCTATC 
AGTGCAATCC CTGCCAGGTG GCTGAGGTCG CTGGAGCTCC TGTTTGCCAG TGGCCAGAAC 
AATAAGCTTC TGTATGGTGA ACCCCCCAAG TCCCCGCGCG CCACCCGCAA GTTCTCCTCG 
CCGCCACCCC TGTCCATCAC CAAGACATCG TCGCCGAGCC GCCGGCGGAA GCTCTCCCTG 
AACATCCCCA TCATCACCGG CGGCAAGGCC CTGGACCTGG CCGCCCTCAG CTGCAACTCC 
AATGGCTACA CCAGCATGTA CTCGGCCATG TCGCCCTTCA GCAAAGCCAC GCTGGACACC 
AGCAAGCTCT ATGTGTCCAG CAGCTTCACC AACAAGATTC CAGATGAGGG CGATACGACC 
CCTGAGAAGC CCGAAGACCC TTCATCGCTC AGCAAGCAGA GCTCAGAAGT CTCCGTGAGA 
GAGGAGTCAG ATACTGATCA AAACCAGAGT GATGATGGTG ATACTGAAAC ATCACCAACT 
AAATCTCCAA CAACACCCAA ATCAGTCAAA AGCAAAAATT CTTCAGAGTT CCCGCTCTTT 
TCCTATAACA ATGGAGTCGT CATGACCTCC TGTCGCGAGC TGGACAATAA CCGCAGTGCC 
TTGTCGGCCG CCTCCGCCTT TGCCATAGCA ACTGCGGGGG CCAACGAGGG CACCCCAAAC 
AAGGAGAAGT ACCGGAGGAT GTCCTTAGCC AGTGCAGGGT TTCCCCCAGA CCAGAGGAAT 
GGAGACAAGG AGTTTGTGAT CCGCAGAGCA GCCACCAATC GTGTCTTGAA TGTGCTCCGC 
CACTGGGTGT CCAAGCACTC TCAGGACTTT GAGACCAACG ATGAGCTCAA ATGCAAGGTG 
ATCGGCTTCC TGGAAGAAGT CATGCACGAT CCGGAGCTCC TGACCCAGGA GCGGAAGGCT 
GCAGCCAACA TCATCAGGAC TCTGACCCAG GAGGACCCAG GTGACAACCA GATCACACTG 
GAGGAGATCA CGCAGATGGC TGAAGGCGTG AAGGCTGAGC CCTTTGAAAA CCACTCGGCC 
CTGGAGATCG CGGAGCAGCT GACCCTGCTA GACCACCTCG TCTTCAAGAA GATTCCTTAT 
GAGGAATTCT TCGGACAAGG ATGGATGAAA CTGGAAAAGA ATGAAAGGAC CCCTTATATC 
ATGAAAACCA CTAAGCACTT CAATGACATC AGTAACTTGA TTGCTTCAGA AATCATCCGC 
AATGAGGACA TCAACGCCAG GGTGAGCACC ATCGAGAAGT GGGTGGCCGT GGCTGACATA 
TGCCGCTGCC TCCACAACTA CAATGCCGTG CTGGAGATCA CCTCGTCCAT GAACCGCAGT 
GCAATCTTCC GGCTCAAAAA GACGTGGCTC AAAGTCTCTA AGCAGACTAA AGCTTTGATT 
GATAAGCTCC AAAAGCTTGT GTCATCTGAG GGCAGATTTA AGAATCTCAG AGAAGCTCTG 
AAAAATTGTG ACCCACCCTG TGTCCCTTAC CTGGGGATGT ACCTCACCGA CCTGGCCTTC 
ATCGAGGAGG GGACGCCCAA TTACACGGAA GACGGCCTGG TCAACTTCTC CAAGATGAGG 
ATGATATCCC ATATTATCCG AGAGATTCGC CAGTTTCAAC AAACTGCCTA CAAAATAGAG 
CACCAAGCAA AGGTAACGCA ATATTTACTG GACCAATCTT TTGTAATGGA TGAAGAAAGC 
CTCTACGAGT CTTCTCTCCG AATAGAACCA AAACTCCCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030670740.1
CDS	440..4384
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPSDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKTFQKPEKTKHRLQDHCVCLNNPQRQEGFSRCPTKRSSPSPGRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSSLSKQSSEVSVREESDTDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_030814880.1
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Definition	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030814880.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAAACA TTTCAAAAAC CAGAAAAAAC AAAACACAGG 
CTCCAGGACC ACTGCGTTTG TTTGAACAAT CCCCAGAGAC AAGAGGGATT TAGCAGGTGT 
CCTACTAAAC GCAGCTCTCC AAGCCCTGGG AGGACCAGGG AGGGGACCAG GGAAGCAGAA 
ATGAGCAGGT CTGACGCCTC CTTATATTGT GATGATGTTG ACATTCGCTT CAGCAAAACC 
ATGAACTCCT GCAAAGTGCT GCAGATCCGC TACGCCAGTG TGGAGCGGCT GCTGGAGAGG 
CTGACGGACC TGCGCTTCCT GAGCATCGAC TTCCTCAACA CCTTCCTGCA CTCCTACCGC 
GTCTTCACCA CCGCCATCGT GGTCCTGGAC AAGCTCATTA CCATCTACAA GAAGCCTATC 
AGTGCAATCC CTGCCAGGTG GCTGAGGTCG CTGGAGCTCC TGTTTGCCAG TGGCCAGAAC 
AATAAGCTTC TGTATGGTGA ACCCCCCAAG TCCCCGCGCG CCACCCGCAA GTTCTCCTCG 
CCGCCACCCC TGTCCATCAC CAAGACATCG TCGCCGAGCC GCCGGCGGAA GCTCTCCCTG 
AACATCCCCA TCATCACCGG CGGCAAGGCC CTGGACCTGG CCGCCCTCAG CTGCAACTCC 
AATGGCTACA CCAGCATGTA CTCGGCCATG TCGCCCTTCA GCAAAGCCAC GCTGGACACC 
AGCAAGCTCT ATGTGTCCAG CAGCTTCACC AACAAGATTC CAGATGAGGG CGATACGACC 
CCTGAGAAGC CCGAAGACCC TTCATCGCTC AGCAAGCAGA GCTCAGAAGT CTCCGTGAGA 
GAGGAGTCAG ATACTGATCA AAACCAGAGT GATGATGGTG ATACTGAAAC ATCACCAACT 
AAATCTCCAA CAACACCCAA ATCAGTCAAA AGCAAAAATT CTTCAGAGTT CCCGCTCTTT 
TCCTATAACA ATGGAGTCGT CATGACCTCC TGTCGCGAGC TGGACAATAA CCGCAGTGCC 
TTGTCGGCCG CCTCCGCCTT TGCCATAGCA ACTGCGGGGG CCAACGAGGG CACCCCAAAC 
AAGGAGAAGT ACCGGAGGAT GTCCTTAGCC AGTGCAGGGT TTCCCCCAGA CCAGAGGAAT 
GGAGACAAGG AGTTTGTGAT CCGCAGAGCA GCCACCAATC GTGTCTTGAA TGTGCTCCGC 
CACTGGGTGT CCAAGCACTC TCAGGACTTT GAGACCAACG ATGAGCTCAA ATGCAAGGTG 
ATCGGCTTCC TGGAAGAAGT CATGCACGAT CCGGAGCTCC TGACCCAGGA GCGGAAGGCT 
GCAGCCAACA TCATCAGGAC TCTGACCCAG GAGGACCCAG GTGACAACCA GATCACACTG 
GAGGAGATCA CGCAGATGGC TGAAGGCGTG AAGGCTGAGC CCTTTGAAAA CCACTCGGCC 
CTGGAGATCG CGGAGCAGCT GACCCTGCTA GACCACCTCG TCTTCAAGAA GATTCCTTAT 
GAGGAATTCT TCGGACAAGG ATGGATGAAA CTGGAAAAGA ATGAAAGGAC CCCTTATATC 
ATGAAAACCA CTAAGCACTT CAATGACATC AGTAACTTGA TTGCTTCAGA AATCATCCGC 
AATGAGGACA TCAACGCCAG GGTGAGCACC ATCGAGAAGT GGGTGGCCGT GGCTGACATA 
TGCCGCTGCC TCCACAACTA CAATGCCGTG CTGGAGATCA CCTCGTCCAT GAACCGCAGT 
GCAATCTTCC GGCTCAAAAA GACGTGGCTC AAAGTCTCTA AGCAGACTAA AGCTTTGATT 
GATAAGCTCC AAAAGCTTGT GTCATCTGAG GGCAGATTTA AGAATCTCAG AGAAGCTCTG 
AAAAATTGTG ACCCACCCTG TGTCCCTTAC CTGGGGATGT ACCTCACCGA CCTGGCCTTC 
ATCGAGGAGG GGACGCCCAA TTACACGGAA GACGGCCTGG TCAACTTCTC CAAGATGAGG 
ATGATATCCC ATATTATCCG AGAGATTCGC CAGTTTCAAC AAACTGCCTA CAAAATAGAG 
CACCAAGCAA AGGTAACGCA ATATTTACTG GACCAATCTT TTGTAATGGA TGAAGAAAGC 
CTCTACGAGT CTTCTCTCCG AATAGAACCA AAACTCCCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_030814881.1 ) cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon) -> XP_030670741.1 Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ONo176983
Clone ID Related Accession (Same CDS sequence)	XM_030814881.1
Accession Version	XM_030814881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nomascus leucogenys Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAAACA TTTCAAAAAC CAGAAAAAAC AAAACACAGG 
CTCCAGGACC ACTGCGTTTG TTTGAACAAT CCCCAGAGAC AAGAGGGATT TAGCAGGTGT 
CCTACTAAAC GCAGCTCTCC AAGCCCTGGG AGGACCAGGG AGGGGACCAG GGAAGCAGAA 
ATGAGCAGGT CTGACGCCTC CTTATATTGT GATGATGTTG ACATTCGCTT CAGCAAAACC 
ATGAACTCCT GCAAAGTGCT GCAGATCCGC TACGCCAGTG TGGAGCGGCT GCTGGAGAGG 
CTGACGGACC TGCGCTTCCT GAGCATCGAC TTCCTCAACA CCTTCCTGCA CTCCTACCGC 
GTCTTCACCA CCGCCATCGT GGTCCTGGAC AAGCTCATTA CCATCTACAA GAAGCCTATC 
AGTGCAATCC CTGCCAGGTC GCTGGAGCTC CTGTTTGCCA GTGGCCAGAA CAATAAGCTT 
CTGTATGGTG AACCCCCCAA GTCCCCGCGC GCCACCCGCA AGTTCTCCTC GCCGCCACCC 
CTGTCCATCA CCAAGACATC GTCGCCGAGC CGCCGGCGGA AGCTCTCCCT GAACATCCCC 
ATCATCACCG GCGGCAAGGC CCTGGACCTG GCCGCCCTCA GCTGCAACTC CAATGGCTAC 
ACCAGCATGT ACTCGGCCAT GTCGCCCTTC AGCAAAGCCA CGCTGGACAC CAGCAAGCTC 
TATGTGTCCA GCAGCTTCAC CAACAAGATT CCAGATGAGG GCGATACGAC CCCTGAGAAG 
CCCGAAGACC CTTCATCGCT CAGCAAGCAG AGCTCAGAAG TCTCCGTGAG AGAGGAGTCA 
GATACTGATC AAAACCAGAG TGATGATGGT GATACTGAAA CATCACCAAC TAAATCTCCA 
ACAACACCCA AATCAGTCAA AAGCAAAAAT TCTTCAGAGT TCCCGCTCTT TTCCTATAAC 
AATGGAGTCG TCATGACCTC CTGTCGCGAG CTGGACAATA ACCGCAGTGC CTTGTCGGCC 
GCCTCCGCCT TTGCCATAGC AACTGCGGGG GCCAACGAGG GCACCCCAAA CAAGGAGAAG 
TACCGGAGGA TGTCCTTAGC CAGTGCAGGG TTTCCCCCAG ACCAGAGGAA TGGAGACAAG 
GAGTTTGTGA TCCGCAGAGC AGCCACCAAT CGTGTCTTGA ATGTGCTCCG CCACTGGGTG 
TCCAAGCACT CTCAGGACTT TGAGACCAAC GATGAGCTCA AATGCAAGGT GATCGGCTTC 
CTGGAAGAAG TCATGCACGA TCCGGAGCTC CTGACCCAGG AGCGGAAGGC TGCAGCCAAC 
ATCATCAGGA CTCTGACCCA GGAGGACCCA GGTGACAACC AGATCACACT GGAGGAGATC 
ACGCAGATGG CTGAAGGCGT GAAGGCTGAG CCCTTTGAAA ACCACTCGGC CCTGGAGATC 
GCGGAGCAGC TGACCCTGCT AGACCACCTC GTCTTCAAGA AGATTCCTTA TGAGGAATTC 
TTCGGACAAG GATGGATGAA ACTGGAAAAG AATGAAAGGA CCCCTTATAT CATGAAAACC 
ACTAAGCACT TCAATGACAT CAGTAACTTG ATTGCTTCAG AAATCATCCG CAATGAGGAC 
ATCAACGCCA GGGTGAGCAC CATCGAGAAG TGGGTGGCCG TGGCTGACAT ATGCCGCTGC 
CTCCACAACT ACAATGCCGT GCTGGAGATC ACCTCGTCCA TGAACCGCAG TGCAATCTTC 
CGGCTCAAAA AGACGTGGCT CAAAGTCTCT AAGCAGACTA AAGCTTTGAT TGATAAGCTC 
CAAAAGCTTG TGTCATCTGA GGGCAGATTT AAGAATCTCA GAGAAGCTCT GAAAAATTGT 
GACCCACCCT GTGTCCCTTA CCTGGGGATG TACCTCACCG ACCTGGCCTT CATCGAGGAG 
GGGACGCCCA ATTACACGGA AGACGGCCTG GTCAACTTCT CCAAGATGAG GATGATATCC 
CATATTATCC GAGAGATTCG CCAGTTTCAA CAAACTGCCT ACAAAATAGA GCACCAAGCA 
AAGGTAACGC AATATTTACT GGACCAATCT TTTGTAATGG ATGAAGAAAG CCTCTACGAG 
TCTTCTCTCC GAATAGAACC AAAACTCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030670741.1
CDS	440..4375
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPSDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKTFQKPEKTKHRLQDHCVCLNNPQRQEGFSRCPTKRSSPSPGRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSSLSKQSSEVSVREESDTDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_030814881.1
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Definition	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030814881.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAAACA TTTCAAAAAC CAGAAAAAAC AAAACACAGG 
CTCCAGGACC ACTGCGTTTG TTTGAACAAT CCCCAGAGAC AAGAGGGATT TAGCAGGTGT 
CCTACTAAAC GCAGCTCTCC AAGCCCTGGG AGGACCAGGG AGGGGACCAG GGAAGCAGAA 
ATGAGCAGGT CTGACGCCTC CTTATATTGT GATGATGTTG ACATTCGCTT CAGCAAAACC 
ATGAACTCCT GCAAAGTGCT GCAGATCCGC TACGCCAGTG TGGAGCGGCT GCTGGAGAGG 
CTGACGGACC TGCGCTTCCT GAGCATCGAC TTCCTCAACA CCTTCCTGCA CTCCTACCGC 
GTCTTCACCA CCGCCATCGT GGTCCTGGAC AAGCTCATTA CCATCTACAA GAAGCCTATC 
AGTGCAATCC CTGCCAGGTC GCTGGAGCTC CTGTTTGCCA GTGGCCAGAA CAATAAGCTT 
CTGTATGGTG AACCCCCCAA GTCCCCGCGC GCCACCCGCA AGTTCTCCTC GCCGCCACCC 
CTGTCCATCA CCAAGACATC GTCGCCGAGC CGCCGGCGGA AGCTCTCCCT GAACATCCCC 
ATCATCACCG GCGGCAAGGC CCTGGACCTG GCCGCCCTCA GCTGCAACTC CAATGGCTAC 
ACCAGCATGT ACTCGGCCAT GTCGCCCTTC AGCAAAGCCA CGCTGGACAC CAGCAAGCTC 
TATGTGTCCA GCAGCTTCAC CAACAAGATT CCAGATGAGG GCGATACGAC CCCTGAGAAG 
CCCGAAGACC CTTCATCGCT CAGCAAGCAG AGCTCAGAAG TCTCCGTGAG AGAGGAGTCA 
GATACTGATC AAAACCAGAG TGATGATGGT GATACTGAAA CATCACCAAC TAAATCTCCA 
ACAACACCCA AATCAGTCAA AAGCAAAAAT TCTTCAGAGT TCCCGCTCTT TTCCTATAAC 
AATGGAGTCG TCATGACCTC CTGTCGCGAG CTGGACAATA ACCGCAGTGC CTTGTCGGCC 
GCCTCCGCCT TTGCCATAGC AACTGCGGGG GCCAACGAGG GCACCCCAAA CAAGGAGAAG 
TACCGGAGGA TGTCCTTAGC CAGTGCAGGG TTTCCCCCAG ACCAGAGGAA TGGAGACAAG 
GAGTTTGTGA TCCGCAGAGC AGCCACCAAT CGTGTCTTGA ATGTGCTCCG CCACTGGGTG 
TCCAAGCACT CTCAGGACTT TGAGACCAAC GATGAGCTCA AATGCAAGGT GATCGGCTTC 
CTGGAAGAAG TCATGCACGA TCCGGAGCTC CTGACCCAGG AGCGGAAGGC TGCAGCCAAC 
ATCATCAGGA CTCTGACCCA GGAGGACCCA GGTGACAACC AGATCACACT GGAGGAGATC 
ACGCAGATGG CTGAAGGCGT GAAGGCTGAG CCCTTTGAAA ACCACTCGGC CCTGGAGATC 
GCGGAGCAGC TGACCCTGCT AGACCACCTC GTCTTCAAGA AGATTCCTTA TGAGGAATTC 
TTCGGACAAG GATGGATGAA ACTGGAAAAG AATGAAAGGA CCCCTTATAT CATGAAAACC 
ACTAAGCACT TCAATGACAT CAGTAACTTG ATTGCTTCAG AAATCATCCG CAATGAGGAC 
ATCAACGCCA GGGTGAGCAC CATCGAGAAG TGGGTGGCCG TGGCTGACAT ATGCCGCTGC 
CTCCACAACT ACAATGCCGT GCTGGAGATC ACCTCGTCCA TGAACCGCAG TGCAATCTTC 
CGGCTCAAAA AGACGTGGCT CAAAGTCTCT AAGCAGACTA AAGCTTTGAT TGATAAGCTC 
CAAAAGCTTG TGTCATCTGA GGGCAGATTT AAGAATCTCA GAGAAGCTCT GAAAAATTGT 
GACCCACCCT GTGTCCCTTA CCTGGGGATG TACCTCACCG ACCTGGCCTT CATCGAGGAG 
GGGACGCCCA ATTACACGGA AGACGGCCTG GTCAACTTCT CCAAGATGAG GATGATATCC 
CATATTATCC GAGAGATTCG CCAGTTTCAA CAAACTGCCT ACAAAATAGA GCACCAAGCA 
AAGGTAACGC AATATTTACT GGACCAATCT TTTGTAATGG ATGAAGAAAG CCTCTACGAG 
TCTTCTCTCC GAATAGAACC AAAACTCCCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_030814882.1 ) cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon) -> XP_030670742.1 Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

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CloneID	ONo176984
Clone ID Related Accession (Same CDS sequence)	XM_030814882.1
Accession Version	XM_030814882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3906bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nomascus leucogenys Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAAACA TTTCAAAAAC CAGAAAAAAC AAAACACAGG 
CTCCAGGACC ACTGCGTTTG TTTGAACAAT CCCCAGAGAC AAGAGGGATT TAGCAGGTGT 
CCTACTAAAC GCAGCTCTCC AAGCCCTGGG TCTGACGCCT CCTTATATTG TGATGATGTT 
GACATTCGCT TCAGCAAAAC CATGAACTCC TGCAAAGTGC TGCAGATCCG CTACGCCAGT 
GTGGAGCGGC TGCTGGAGAG GCTGACGGAC CTGCGCTTCC TGAGCATCGA CTTCCTCAAC 
ACCTTCCTGC ACTCCTACCG CGTCTTCACC ACCGCCATCG TGGTCCTGGA CAAGCTCATT 
ACCATCTACA AGAAGCCTAT CAGTGCAATC CCTGCCAGGT GGCTGAGGTC GCTGGAGCTC 
CTGTTTGCCA GTGGCCAGAA CAATAAGCTT CTGTATGGTG AACCCCCCAA GTCCCCGCGC 
GCCACCCGCA AGTTCTCCTC GCCGCCACCC CTGTCCATCA CCAAGACATC GTCGCCGAGC 
CGCCGGCGGA AGCTCTCCCT GAACATCCCC ATCATCACCG GCGGCAAGGC CCTGGACCTG 
GCCGCCCTCA GCTGCAACTC CAATGGCTAC ACCAGCATGT ACTCGGCCAT GTCGCCCTTC 
AGCAAAGCCA CGCTGGACAC CAGCAAGCTC TATGTGTCCA GCAGCTTCAC CAACAAGATT 
CCAGATGAGG GCGATACGAC CCCTGAGAAG CCCGAAGACC CTTCATCGCT CAGCAAGCAG 
AGCTCAGAAG TCTCCGTGAG AGAGGAGTCA GATACTGATC AAAACCAGAG TGATGATGGT 
GATACTGAAA CATCACCAAC TAAATCTCCA ACAACACCCA AATCAGTCAA AAGCAAAAAT 
TCTTCAGAGT TCCCGCTCTT TTCCTATAAC AATGGAGTCG TCATGACCTC CTGTCGCGAG 
CTGGACAATA ACCGCAGTGC CTTGTCGGCC GCCTCCGCCT TTGCCATAGC AACTGCGGGG 
GCCAACGAGG GCACCCCAAA CAAGGAGAAG TACCGGAGGA TGTCCTTAGC CAGTGCAGGG 
TTTCCCCCAG ACCAGAGGAA TGGAGACAAG GAGTTTGTGA TCCGCAGAGC AGCCACCAAT 
CGTGTCTTGA ATGTGCTCCG CCACTGGGTG TCCAAGCACT CTCAGGACTT TGAGACCAAC 
GATGAGCTCA AATGCAAGGT GATCGGCTTC CTGGAAGAAG TCATGCACGA TCCGGAGCTC 
CTGACCCAGG AGCGGAAGGC TGCAGCCAAC ATCATCAGGA CTCTGACCCA GGAGGACCCA 
GGTGACAACC AGATCACACT GGAGGAGATC ACGCAGATGG CTGAAGGCGT GAAGGCTGAG 
CCCTTTGAAA ACCACTCGGC CCTGGAGATC GCGGAGCAGC TGACCCTGCT AGACCACCTC 
GTCTTCAAGA AGATTCCTTA TGAGGAATTC TTCGGACAAG GATGGATGAA ACTGGAAAAG 
AATGAAAGGA CCCCTTATAT CATGAAAACC ACTAAGCACT TCAATGACAT CAGTAACTTG 
ATTGCTTCAG AAATCATCCG CAATGAGGAC ATCAACGCCA GGGTGAGCAC CATCGAGAAG 
TGGGTGGCCG TGGCTGACAT ATGCCGCTGC CTCCACAACT ACAATGCCGT GCTGGAGATC 
ACCTCGTCCA TGAACCGCAG TGCAATCTTC CGGCTCAAAA AGACGTGGCT CAAAGTCTCT 
AAGCAGACTA AAGCTTTGAT TGATAAGCTC CAAAAGCTTG TGTCATCTGA GGGCAGATTT 
AAGAATCTCA GAGAAGCTCT GAAAAATTGT GACCCACCCT GTGTCCCTTA CCTGGGGATG 
TACCTCACCG ACCTGGCCTT CATCGAGGAG GGGACGCCCA ATTACACGGA AGACGGCCTG 
GTCAACTTCT CCAAGATGAG GATGATATCC CATATTATCC GAGAGATTCG CCAGTTTCAA 
CAAACTGCCT ACAAAATAGA GCACCAAGCA AAGGTAACGC AATATTTACT GGACCAATCT 
TTTGTAATGG ATGAAGAAAG CCTCTACGAG TCTTCTCTCC GAATAGAACC AAAACTCCCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030670742.1
CDS	440..4345
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPSDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKTFQKPEKTKHRLQDHCVCLNNPQRQEGFSRCPTKRSSPSPGSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSSLSKQSSEVSVREESDTDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_030814882.1
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Definition	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030814882.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAAACA TTTCAAAAAC CAGAAAAAAC AAAACACAGG 
CTCCAGGACC ACTGCGTTTG TTTGAACAAT CCCCAGAGAC AAGAGGGATT TAGCAGGTGT 
CCTACTAAAC GCAGCTCTCC AAGCCCTGGG TCTGACGCCT CCTTATATTG TGATGATGTT 
GACATTCGCT TCAGCAAAAC CATGAACTCC TGCAAAGTGC TGCAGATCCG CTACGCCAGT 
GTGGAGCGGC TGCTGGAGAG GCTGACGGAC CTGCGCTTCC TGAGCATCGA CTTCCTCAAC 
ACCTTCCTGC ACTCCTACCG CGTCTTCACC ACCGCCATCG TGGTCCTGGA CAAGCTCATT 
ACCATCTACA AGAAGCCTAT CAGTGCAATC CCTGCCAGGT GGCTGAGGTC GCTGGAGCTC 
CTGTTTGCCA GTGGCCAGAA CAATAAGCTT CTGTATGGTG AACCCCCCAA GTCCCCGCGC 
GCCACCCGCA AGTTCTCCTC GCCGCCACCC CTGTCCATCA CCAAGACATC GTCGCCGAGC 
CGCCGGCGGA AGCTCTCCCT GAACATCCCC ATCATCACCG GCGGCAAGGC CCTGGACCTG 
GCCGCCCTCA GCTGCAACTC CAATGGCTAC ACCAGCATGT ACTCGGCCAT GTCGCCCTTC 
AGCAAAGCCA CGCTGGACAC CAGCAAGCTC TATGTGTCCA GCAGCTTCAC CAACAAGATT 
CCAGATGAGG GCGATACGAC CCCTGAGAAG CCCGAAGACC CTTCATCGCT CAGCAAGCAG 
AGCTCAGAAG TCTCCGTGAG AGAGGAGTCA GATACTGATC AAAACCAGAG TGATGATGGT 
GATACTGAAA CATCACCAAC TAAATCTCCA ACAACACCCA AATCAGTCAA AAGCAAAAAT 
TCTTCAGAGT TCCCGCTCTT TTCCTATAAC AATGGAGTCG TCATGACCTC CTGTCGCGAG 
CTGGACAATA ACCGCAGTGC CTTGTCGGCC GCCTCCGCCT TTGCCATAGC AACTGCGGGG 
GCCAACGAGG GCACCCCAAA CAAGGAGAAG TACCGGAGGA TGTCCTTAGC CAGTGCAGGG 
TTTCCCCCAG ACCAGAGGAA TGGAGACAAG GAGTTTGTGA TCCGCAGAGC AGCCACCAAT 
CGTGTCTTGA ATGTGCTCCG CCACTGGGTG TCCAAGCACT CTCAGGACTT TGAGACCAAC 
GATGAGCTCA AATGCAAGGT GATCGGCTTC CTGGAAGAAG TCATGCACGA TCCGGAGCTC 
CTGACCCAGG AGCGGAAGGC TGCAGCCAAC ATCATCAGGA CTCTGACCCA GGAGGACCCA 
GGTGACAACC AGATCACACT GGAGGAGATC ACGCAGATGG CTGAAGGCGT GAAGGCTGAG 
CCCTTTGAAA ACCACTCGGC CCTGGAGATC GCGGAGCAGC TGACCCTGCT AGACCACCTC 
GTCTTCAAGA AGATTCCTTA TGAGGAATTC TTCGGACAAG GATGGATGAA ACTGGAAAAG 
AATGAAAGGA CCCCTTATAT CATGAAAACC ACTAAGCACT TCAATGACAT CAGTAACTTG 
ATTGCTTCAG AAATCATCCG CAATGAGGAC ATCAACGCCA GGGTGAGCAC CATCGAGAAG 
TGGGTGGCCG TGGCTGACAT ATGCCGCTGC CTCCACAACT ACAATGCCGT GCTGGAGATC 
ACCTCGTCCA TGAACCGCAG TGCAATCTTC CGGCTCAAAA AGACGTGGCT CAAAGTCTCT 
AAGCAGACTA AAGCTTTGAT TGATAAGCTC CAAAAGCTTG TGTCATCTGA GGGCAGATTT 
AAGAATCTCA GAGAAGCTCT GAAAAATTGT GACCCACCCT GTGTCCCTTA CCTGGGGATG 
TACCTCACCG ACCTGGCCTT CATCGAGGAG GGGACGCCCA ATTACACGGA AGACGGCCTG 
GTCAACTTCT CCAAGATGAG GATGATATCC CATATTATCC GAGAGATTCG CCAGTTTCAA 
CAAACTGCCT ACAAAATAGA GCACCAAGCA AAGGTAACGC AATATTTACT GGACCAATCT 
TTTGTAATGG ATGAAGAAAG CCTCTACGAG TCTTCTCTCC GAATAGAACC AAAACTCCCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_030814883.1 ) cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon) -> XP_030670743.1 Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ONo176985
Clone ID Related Accession (Same CDS sequence)	XM_030814883.1
Accession Version	XM_030814883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nomascus leucogenys Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCTG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCAATCCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT 
AAGCTTCTGT ATGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG 
CCACCCCTGT CCATCACCAA GACATCGTCG CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC 
ATCCCCATCA TCACCGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT 
GGCTACACCA GCATGTACTC GGCCATGTCG CCCTTCAGCA AAGCCACGCT GGACACCAGC 
AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT 
GAGAAGCCCG AAGACCCTTC ATCGCTCAGC AAGCAGAGCT CAGAAGTCTC CGTGAGAGAG 
GAGTCAGATA CTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA 
TCTCCAACAA CACCCAAATC AGTCAAAAGC AAAAATTCTT CAGAGTTCCC GCTCTTTTCC 
TATAACAATG GAGTCGTCAT GACCTCCTGT CGCGAGCTGG ACAATAACCG CAGTGCCTTG 
TCGGCCGCCT CCGCCTTTGC CATAGCAACT GCGGGGGCCA ACGAGGGCAC CCCAAACAAG 
GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA 
GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAATGT GCTCCGCCAC 
TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC 
GGCTTCCTGG AAGAAGTCAT GCACGATCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA 
GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACACTGGAG 
GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCGGCCCTG 
GAGATCGCGG AGCAGCTGAC CCTGCTAGAC CACCTCGTCT TCAAGAAGAT TCCTTATGAG 
GAATTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG 
AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT 
GAGGACATCA ACGCCAGGGT GAGCACCATC GAGAAGTGGG TGGCCGTGGC TGACATATGC 
CGCTGCCTCC ACAACTACAA TGCCGTGCTG GAGATCACCT CGTCCATGAA CCGCAGTGCA 
ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT 
AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA 
AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC 
GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG 
ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC 
CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC 
TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030670743.1
CDS	440..4261
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPSDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSSLSKQSSEVSVREESDTDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_030814883.1
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Definition	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030814883.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCTG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCAATCCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT 
AAGCTTCTGT ATGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG 
CCACCCCTGT CCATCACCAA GACATCGTCG CCGAGCCGCC GGCGGAAGCT CTCCCTGAAC 
ATCCCCATCA TCACCGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT 
GGCTACACCA GCATGTACTC GGCCATGTCG CCCTTCAGCA AAGCCACGCT GGACACCAGC 
AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT 
GAGAAGCCCG AAGACCCTTC ATCGCTCAGC AAGCAGAGCT CAGAAGTCTC CGTGAGAGAG 
GAGTCAGATA CTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA 
TCTCCAACAA CACCCAAATC AGTCAAAAGC AAAAATTCTT CAGAGTTCCC GCTCTTTTCC 
TATAACAATG GAGTCGTCAT GACCTCCTGT CGCGAGCTGG ACAATAACCG CAGTGCCTTG 
TCGGCCGCCT CCGCCTTTGC CATAGCAACT GCGGGGGCCA ACGAGGGCAC CCCAAACAAG 
GAGAAGTACC GGAGGATGTC CTTAGCCAGT GCAGGGTTTC CCCCAGACCA GAGGAATGGA 
GACAAGGAGT TTGTGATCCG CAGAGCAGCC ACCAATCGTG TCTTGAATGT GCTCCGCCAC 
TGGGTGTCCA AGCACTCTCA GGACTTTGAG ACCAACGATG AGCTCAAATG CAAGGTGATC 
GGCTTCCTGG AAGAAGTCAT GCACGATCCG GAGCTCCTGA CCCAGGAGCG GAAGGCTGCA 
GCCAACATCA TCAGGACTCT GACCCAGGAG GACCCAGGTG ACAACCAGAT CACACTGGAG 
GAGATCACGC AGATGGCTGA AGGCGTGAAG GCTGAGCCCT TTGAAAACCA CTCGGCCCTG 
GAGATCGCGG AGCAGCTGAC CCTGCTAGAC CACCTCGTCT TCAAGAAGAT TCCTTATGAG 
GAATTCTTCG GACAAGGATG GATGAAACTG GAAAAGAATG AAAGGACCCC TTATATCATG 
AAAACCACTA AGCACTTCAA TGACATCAGT AACTTGATTG CTTCAGAAAT CATCCGCAAT 
GAGGACATCA ACGCCAGGGT GAGCACCATC GAGAAGTGGG TGGCCGTGGC TGACATATGC 
CGCTGCCTCC ACAACTACAA TGCCGTGCTG GAGATCACCT CGTCCATGAA CCGCAGTGCA 
ATCTTCCGGC TCAAAAAGAC GTGGCTCAAA GTCTCTAAGC AGACTAAAGC TTTGATTGAT 
AAGCTCCAAA AGCTTGTGTC ATCTGAGGGC AGATTTAAGA ATCTCAGAGA AGCTCTGAAA 
AATTGTGACC CACCCTGTGT CCCTTACCTG GGGATGTACC TCACCGACCT GGCCTTCATC 
GAGGAGGGGA CGCCCAATTA CACGGAAGAC GGCCTGGTCA ACTTCTCCAA GATGAGGATG 
ATATCCCATA TTATCCGAGA GATTCGCCAG TTTCAACAAA CTGCCTACAA AATAGAGCAC 
CAAGCAAAGG TAACGCAATA TTTACTGGAC CAATCTTTTG TAATGGATGA AGAAAGCCTC 
TACGAGTCTT CTCTCCGAAT AGAACCAAAA CTCCCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_030814884.1 ) cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon) -> XP_030670744.1 Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ONo176986
Clone ID Related Accession (Same CDS sequence)	XM_030814884.1
Accession Version	XM_030814884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nomascus leucogenys Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGTCT GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCAATCCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG 
TTTGCCAGTG GCCAGAACAA TAAGCTTCTG TATGGTGAAC CCCCCAAGTC CCCGCGCGCC 
ACCCGCAAGT TCTCCTCGCC GCCACCCCTG TCCATCACCA AGACATCGTC GCCGAGCCGC 
CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACCGGCG GCAAGGCCCT GGACCTGGCC 
GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC GCCCTTCAGC 
AAAGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA 
GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CATCGCTCAG CAAGCAGAGC 
TCAGAAGTCT CCGTGAGAGA GGAGTCAGAT ACTGATCAAA ACCAGAGTGA TGATGGTGAT 
ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAG CAAAAATTCT 
TCAGAGTTCC CGCTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGCGAGCTG 
GACAATAACC GCAGTGCCTT GTCGGCCGCC TCCGCCTTTG CCATAGCAAC TGCGGGGGCC 
AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT 
CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT 
GTCTTGAATG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT 
GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGATCC GGAGCTCCTG 
ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT 
GACAACCAGA TCACACTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC 
TTTGAAAACC ACTCGGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA CCACCTCGTC 
TTCAAGAAGA TTCCTTATGA GGAATTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT 
GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT 
GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCACCAT CGAGAAGTGG 
GTGGCCGTGG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTGCT GGAGATCACC 
TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG 
CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG 
AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC 
CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC 
AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA 
ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT 
GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030670744.1
CDS	440..4222
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPSDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSSLSKQSSEVSVREESDTDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_030814884.1
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Definition	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030814884.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGTCT GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCAATCCCT GCCAGGTGGC TGAGGTCGCT GGAGCTCCTG 
TTTGCCAGTG GCCAGAACAA TAAGCTTCTG TATGGTGAAC CCCCCAAGTC CCCGCGCGCC 
ACCCGCAAGT TCTCCTCGCC GCCACCCCTG TCCATCACCA AGACATCGTC GCCGAGCCGC 
CGGCGGAAGC TCTCCCTGAA CATCCCCATC ATCACCGGCG GCAAGGCCCT GGACCTGGCC 
GCCCTCAGCT GCAACTCCAA TGGCTACACC AGCATGTACT CGGCCATGTC GCCCTTCAGC 
AAAGCCACGC TGGACACCAG CAAGCTCTAT GTGTCCAGCA GCTTCACCAA CAAGATTCCA 
GATGAGGGCG ATACGACCCC TGAGAAGCCC GAAGACCCTT CATCGCTCAG CAAGCAGAGC 
TCAGAAGTCT CCGTGAGAGA GGAGTCAGAT ACTGATCAAA ACCAGAGTGA TGATGGTGAT 
ACTGAAACAT CACCAACTAA ATCTCCAACA ACACCCAAAT CAGTCAAAAG CAAAAATTCT 
TCAGAGTTCC CGCTCTTTTC CTATAACAAT GGAGTCGTCA TGACCTCCTG TCGCGAGCTG 
GACAATAACC GCAGTGCCTT GTCGGCCGCC TCCGCCTTTG CCATAGCAAC TGCGGGGGCC 
AACGAGGGCA CCCCAAACAA GGAGAAGTAC CGGAGGATGT CCTTAGCCAG TGCAGGGTTT 
CCCCCAGACC AGAGGAATGG AGACAAGGAG TTTGTGATCC GCAGAGCAGC CACCAATCGT 
GTCTTGAATG TGCTCCGCCA CTGGGTGTCC AAGCACTCTC AGGACTTTGA GACCAACGAT 
GAGCTCAAAT GCAAGGTGAT CGGCTTCCTG GAAGAAGTCA TGCACGATCC GGAGCTCCTG 
ACCCAGGAGC GGAAGGCTGC AGCCAACATC ATCAGGACTC TGACCCAGGA GGACCCAGGT 
GACAACCAGA TCACACTGGA GGAGATCACG CAGATGGCTG AAGGCGTGAA GGCTGAGCCC 
TTTGAAAACC ACTCGGCCCT GGAGATCGCG GAGCAGCTGA CCCTGCTAGA CCACCTCGTC 
TTCAAGAAGA TTCCTTATGA GGAATTCTTC GGACAAGGAT GGATGAAACT GGAAAAGAAT 
GAAAGGACCC CTTATATCAT GAAAACCACT AAGCACTTCA ATGACATCAG TAACTTGATT 
GCTTCAGAAA TCATCCGCAA TGAGGACATC AACGCCAGGG TGAGCACCAT CGAGAAGTGG 
GTGGCCGTGG CTGACATATG CCGCTGCCTC CACAACTACA ATGCCGTGCT GGAGATCACC 
TCGTCCATGA ACCGCAGTGC AATCTTCCGG CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG 
CAGACTAAAG CTTTGATTGA TAAGCTCCAA AAGCTTGTGT CATCTGAGGG CAGATTTAAG 
AATCTCAGAG AAGCTCTGAA AAATTGTGAC CCACCCTGTG TCCCTTACCT GGGGATGTAC 
CTCACCGACC TGGCCTTCAT CGAGGAGGGG ACGCCCAATT ACACGGAAGA CGGCCTGGTC 
AACTTCTCCA AGATGAGGAT GATATCCCAT ATTATCCGAG AGATTCGCCA GTTTCAACAA 
ACTGCCTACA AAATAGAGCA CCAAGCAAAG GTAACGCAAT ATTTACTGGA CCAATCTTTT 
GTAATGGATG AAGAAAGCCT CTACGAGTCT TCTCTCCGAA TAGAACCAAA ACTCCCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_030814885.1 ) cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon) -> XP_030670745.1 Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X6, mRNA.

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CloneID	ONo176987
Clone ID Related Accession (Same CDS sequence)	XM_030814885.1
Accession Version	XM_030814885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-16
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Product	ras-specific guanine nucleotide-releasing factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_044386.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Nomascus leucogenys Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGTCT GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCAATCCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT 
GGCCAGAACA ATAAGCTTCT GTATGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACATCGT CGCCGAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACCGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTCAG CAAAGCCACG 
CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC 
GATACGACCC CTGAGAAGCC CGAAGACCCT TCATCGCTCA GCAAGCAGAG CTCAGAAGTC 
TCCGTGAGAG AGGAGTCAGA TACTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA 
TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA GCAAAAATTC TTCAGAGTTC 
CCGCTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGCGAGCT GGACAATAAC 
CGCAGTGCCT TGTCGGCCGC CTCCGCCTTT GCCATAGCAA CTGCGGGGGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC 
CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAT 
GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA 
TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGATC CGGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG 
ATCACACTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC 
CACTCGGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ACCACCTCGT CTTCAAGAAG 
ATTCCTTATG AGGAATTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC 
CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA 
ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCACCA TCGAGAAGTG GGTGGCCGTG 
GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTGC TGGAGATCAC CTCGTCCATG 
AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA 
GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC 
AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030670745.1
CDS	440..4213
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPSDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMHDEVSETENIRKNLAIERMIIEGCEILLDTSQTFVRQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSSLSKQSSEVSVREESDTDQNQSDDGDTETSPTKSPTTPKSVKSKNSSEFPLFSYNNGVVMTSCRELDNNRSALSAASAFAIATAGANEGTPNKEKYRRMSLASAGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_030814885.1
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Definition	Nomascus leucogenys Ras protein specific guanine nucleotide releasing factor 1 (RASGRF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030814885.1

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC ACGATGAAGT AAGTGAGACG GAGAACATCC GGAAAAACCT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTC CTGGACACCA GCCAGACCTT TGTGAGACAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGTCT GACGCCTCCT TATATTGTGA TGATGTTGAC 
ATTCGCTTCA GCAAAACCAT GAACTCCTGC AAAGTGCTGC AGATCCGCTA CGCCAGTGTG 
GAGCGGCTGC TGGAGAGGCT GACGGACCTG CGCTTCCTGA GCATCGACTT CCTCAACACC 
TTCCTGCACT CCTACCGCGT CTTCACCACC GCCATCGTGG TCCTGGACAA GCTCATTACC 
ATCTACAAGA AGCCTATCAG TGCAATCCCT GCCAGGTCGC TGGAGCTCCT GTTTGCCAGT 
GGCCAGAACA ATAAGCTTCT GTATGGTGAA CCCCCCAAGT CCCCGCGCGC CACCCGCAAG 
TTCTCCTCGC CGCCACCCCT GTCCATCACC AAGACATCGT CGCCGAGCCG CCGGCGGAAG 
CTCTCCCTGA ACATCCCCAT CATCACCGGC GGCAAGGCCC TGGACCTGGC CGCCCTCAGC 
TGCAACTCCA ATGGCTACAC CAGCATGTAC TCGGCCATGT CGCCCTTCAG CAAAGCCACG 
CTGGACACCA GCAAGCTCTA TGTGTCCAGC AGCTTCACCA ACAAGATTCC AGATGAGGGC 
GATACGACCC CTGAGAAGCC CGAAGACCCT TCATCGCTCA GCAAGCAGAG CTCAGAAGTC 
TCCGTGAGAG AGGAGTCAGA TACTGATCAA AACCAGAGTG ATGATGGTGA TACTGAAACA 
TCACCAACTA AATCTCCAAC AACACCCAAA TCAGTCAAAA GCAAAAATTC TTCAGAGTTC 
CCGCTCTTTT CCTATAACAA TGGAGTCGTC ATGACCTCCT GTCGCGAGCT GGACAATAAC 
CGCAGTGCCT TGTCGGCCGC CTCCGCCTTT GCCATAGCAA CTGCGGGGGC CAACGAGGGC 
ACCCCAAACA AGGAGAAGTA CCGGAGGATG TCCTTAGCCA GTGCAGGGTT TCCCCCAGAC 
CAGAGGAATG GAGACAAGGA GTTTGTGATC CGCAGAGCAG CCACCAATCG TGTCTTGAAT 
GTGCTCCGCC ACTGGGTGTC CAAGCACTCT CAGGACTTTG AGACCAACGA TGAGCTCAAA 
TGCAAGGTGA TCGGCTTCCT GGAAGAAGTC ATGCACGATC CGGAGCTCCT GACCCAGGAG 
CGGAAGGCTG CAGCCAACAT CATCAGGACT CTGACCCAGG AGGACCCAGG TGACAACCAG 
ATCACACTGG AGGAGATCAC GCAGATGGCT GAAGGCGTGA AGGCTGAGCC CTTTGAAAAC 
CACTCGGCCC TGGAGATCGC GGAGCAGCTG ACCCTGCTAG ACCACCTCGT CTTCAAGAAG 
ATTCCTTATG AGGAATTCTT CGGACAAGGA TGGATGAAAC TGGAAAAGAA TGAAAGGACC 
CCTTATATCA TGAAAACCAC TAAGCACTTC AATGACATCA GTAACTTGAT TGCTTCAGAA 
ATCATCCGCA ATGAGGACAT CAACGCCAGG GTGAGCACCA TCGAGAAGTG GGTGGCCGTG 
GCTGACATAT GCCGCTGCCT CCACAACTAC AATGCCGTGC TGGAGATCAC CTCGTCCATG 
AACCGCAGTG CAATCTTCCG GCTCAAAAAG ACGTGGCTCA AAGTCTCTAA GCAGACTAAA 
GCTTTGATTG ATAAGCTCCA AAAGCTTGTG TCATCTGAGG GCAGATTTAA GAATCTCAGA 
GAAGCTCTGA AAAATTGTGA CCCACCCTGT GTCCCTTACC TGGGGATGTA CCTCACCGAC 
CTGGCCTTCA TCGAGGAGGG GACGCCCAAT TACACGGAAG ACGGCCTGGT CAACTTCTCC 
AAGATGAGGA TGATATCCCA TATTATCCGA GAGATTCGCC AGTTTCAACA AACTGCCTAC 
AAAATAGAGC ACCAAGCAAA GGTAACGCAA TATTTACTGG ACCAATCTTT TGTAATGGAT 
GAAGAAAGCC TCTACGAGTC TTCTCTCCGA ATAGAACCAA AACTCCCCAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASGRF1 ( XM_003275431.3 ) cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon) -> XP_003275479.1 Nomascus leucogenys Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), mRNA.

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CloneID	ONo65853
Clone ID Related Accession (Same CDS sequence)	XM_003275431.3
Accession Version	XM_003275431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-12
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Product	LOW QUALITY PROTEIN: ras-specific guanine nucleotide-releasing factor 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004087887.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2015 this sequence version replaced XM_003275431.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nomascus leucogenys Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC AAGATGAAGT AAGTGAGAAG GAGAACATCT GGAAAAAGAT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTG AGGGACACCA GCCAGACGTT TGTGAGTCAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCTG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCAATCCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT 
AAGCTTCTGT ATGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG 
CCACCCCTGT CCATCACCAA GACATCGTCG CCCAGCCGCC GGCGGAAGCT CTCCCTGAAC 
ATCCCCATCA TCACCGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT 
GGCTACACCA GCATGTACTC GGCCATGTCG CCCTTCAGCA AAGCCACGCT GGACACCAGC 
AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT 
GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CGTGAGAGAG 
GAGTCAGATA CTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA 
TCTCCAACAA CACCCAAATC AGTCAAAAGC AAAAATTCTT CAGGGGCTAT GAGATACCCA 
ATGGTGAGAG AGAGCTCCCA GGGGACCCAC CTCCCGGGCA GCCCCCTCCT CACACAGCCT 
TCTCCAATGC CCCTGCTCTC ACATGCAAAG GGCCGCCTGC AGCCTGAACT CGCCACTATC 
CAGACCTTCA AAAGAAACCC AATAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG 
TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAATG TGCTCCGCCA CTGGGTGTCC 
AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCTTG 
GAAGAAGTCA TGCACGATCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC 
ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACACTGGA GGAGATCACG 
CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCGGCCCT GGAGATCGCG 
GAGCAGCTGA CCCTGCTAGA CCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAATTCTTC 
GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT 
AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA TGAGGACATC 
AACGCCAGGG TGAGCACCAT CGAGAAGTGG GTGGCCGTGG CTGACATATG CCGCTGCCTC 
CACAACTACA ATGCCGTGCT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG 
CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA 
AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC 
CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG 
ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT 
ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG 
GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT 
TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003275479.1
CDS	440..4252
Translation	MQKGIRLNDGHVASLGLLARKDGTRKGYLSKRSSDNTKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEGCVCDRAPSPKPALSAKEPLEKQHYFTVNFSHENQKALELRTEDAKDCDEWVAAIAHASYRTLATEHEALMQKYLHLLQIVETEKTVAKQLRQQIEDGEIEIERLKAEIASLLKDNERIQSTQTVAPSDEDSDIKKIKKVQSFLRGWLCRRKWKTIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPPITHDDVSSIFLNSETIMFLHQIFYQGLKARISSWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHCKQNRDFDKLLKHYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELSRIMQDEVSEKENIWKKMAIERMIIEGCEILRDTSQTFVSQGSLIQVPMSEKGKITRGRLGSLSLKKEGERQCFLFSKHLIICTRGSGGKLHLTKNGVISLIDCTLLEEPESTEEEAKGSGQDIDHLDFKIGVEPKDSPPFTVILVASSRQEKAAWTSDISQCVDNIRCNGLMMNAFEENSKVTVPQMIKRTREGTREAEMSRSDASLYCDDVDIRFSKTMNSCKVLQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTTAIVVLDKLITIYKKPISAIPARWLRSLELLFASGQNNKLLYGEPPKSPRATRKFSSPPPLSITKTSSPSRRRKLSLNIPIITGGKALDLAALSCNSNGYTSMYSAMSPFSKATLDTSKLYVSSSFTNKIPDEGDTTPEKPEDPSALSKQSSEVSVREESDTDQNQSDDGDTETSPTKSPTTPKSVKSKNSSGAMRYPMVRESSQGTHLPGSPLLTQPSPMPLLSHAKGRLQPELATIQTFKRNPIGFPPDQRNGDKEFVIRRAATNRVLNVLRHWVSKHSQDFETNDELKCKVIGFLEEVMHDPELLTQERKAAANIIRTLTQEDPGDNQITLEEITQMAEGVKAEPFENHSALEIAEQLTLLDHLVFKKIPYEEFFGQGWMKLEKNERTPYIMKTTKHFNDISNLIASEIIRNEDINARVSTIEKWVAVADICRCLHNYNAVLEITSSMNRSAIFRLKKTWLKVSKQTKALIDKLQKLVSSEGRFKNLREALKNCDPPCVPYLGMYLTDLAFIEEGTPNYTEDGLVNFSKMRMISHIIREIRQFQQTAYKIEHQAKVTQYLLDQSFVMDEESLYESSLRIEPKLPT

Target ORF information:

RefSeq Version	XM_003275431.3
Organism	Nomascus leucogenys(northern white-cheeked gibbon)
Definition	Nomascus leucogenys Ras protein-specific guanine nucleotide-releasing factor 1 (RASGRF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003275431.3

ORF Insert Sequence:

ATGCAGAAGG GGATCCGGCT GAATGATGGC CACGTCGCGT CCCTGGGACT GCTGGCGCGC 
AAGGACGGCA CGCGCAAAGG CTACCTGAGC AAGCGGAGTT CGGACAACAC AAAATGGCAA 
ACCAAGTGGT TCGCGCTGCT GCAGAACCTG CTCTTCTACT TCGAGAGCGA CTCGAGCTCG 
CGGCCCTCGG GGCTTTACCT GCTGGAGGGC TGCGTCTGCG ACCGCGCGCC CTCCCCCAAG 
CCGGCGCTGT CGGCCAAGGA GCCGCTGGAG AAACAGCATT ACTTCACGGT GAACTTCAGC 
CATGAGAACC AGAAAGCTTT GGAGCTGAGG ACAGAGGACG CAAAAGATTG TGACGAATGG 
GTGGCAGCCA TTGCACATGC CAGCTACAGG ACCCTGGCCA CAGAGCATGA GGCGTTAATG 
CAGAAGTACC TGCACCTGCT GCAGATCGTG GAGACGGAGA AGACCGTGGC CAAGCAGCTT 
CGGCAGCAGA TCGAGGATGG GGAGATTGAG ATCGAGCGGC TGAAGGCAGA GATCGCATCC 
CTGCTCAAGG ACAATGAGCG CATCCAGTCC ACCCAGACTG TCGCCCCCAG TGATGAAGAC 
AGCGATATCA AGAAAATTAA GAAGGTGCAG AGCTTCCTGC GGGGCTGGCT GTGCCGGCGG 
AAATGGAAGA CCATCATCCA GGACTACATC CGGTCACCCC ATGCCGACAG CATGCGCAAG 
AGGAACCAGG TGGTGTTCAG CATGCTGGAG GCTGAGGCTG AGTACGTGCA GCAGCTGCAC 
ATCCTCGTCA ACAATTTTCT GCGCCCGCTG CGGATGGCCG CCAGCTCCAA GAAGCCTCCC 
ATCACGCACG ACGACGTCAG CAGCATCTTC CTGAACAGCG AAACCATCAT GTTTTTACAT 
CAGATCTTTT ACCAAGGCCT GAAGGCCCGC ATCTCCAGCT GGCCCACGCT GGTCCTGGCT 
GACCTATTTG ACATCCTGCT GCCTATGCTC AACATCTACC AAGAGTTCGT CCGCAACCAC 
CAGTACAGCC TGCAGATCCT GGCCCACTGC AAGCAGAACC GTGACTTTGA CAAGCTGCTG 
AAGCACTACG AGGCCAAGCC TGACTGCGAG GAGAGGACGC TGGAGACCTT CCTCACCTAC 
CCCATGTTCC AGATCCCCAG GTACATCCTG ACCCTCCATG AGCTCCTGGC CCACACGCCT 
CATGAGCACG TTGAGCGCAA CAGCCTGGAC TACGCCAAGT CCAAACTGGA GGAGCTGTCC 
AGAATAATGC AAGATGAAGT AAGTGAGAAG GAGAACATCT GGAAAAAGAT GGCCATCGAG 
CGCATGATCA TCGAAGGCTG TGAGATCCTG AGGGACACCA GCCAGACGTT TGTGAGTCAA 
GGTTCCCTCA TCCAGGTGCC CATGTCTGAA AAGGGCAAGA TCACCAGGGG GCGCCTGGGG 
TCTCTCTCCC TAAAGAAAGA GGGCGAACGA CAGTGCTTTC TGTTTTCTAA GCATCTGATT 
ATCTGTACCA GAGGCTCTGG AGGGAAGCTT CACCTGACCA AGAATGGAGT CATATCCCTC 
ATTGACTGCA CTTTATTGGA GGAGCCAGAA AGCACGGAGG AGGAAGCCAA AGGATCCGGC 
CAAGACATAG ATCACTTGGA TTTTAAAATC GGGGTGGAGC CAAAGGATTC CCCGCCCTTT 
ACAGTCATCC TAGTGGCCTC GTCCAGACAG GAGAAGGCAG CGTGGACCAG TGACATCAGC 
CAGTGTGTGG ATAACATCCG ATGCAATGGG CTCATGATGA ACGCATTTGA AGAAAATTCC 
AAGGTCACTG TGCCACAGAT GATCAAGAGG ACCAGGGAGG GGACCAGGGA AGCAGAAATG 
AGCAGGTCTG ACGCCTCCTT ATATTGTGAT GATGTTGACA TTCGCTTCAG CAAAACCATG 
AACTCCTGCA AAGTGCTGCA GATCCGCTAC GCCAGTGTGG AGCGGCTGCT GGAGAGGCTG 
ACGGACCTGC GCTTCCTGAG CATCGACTTC CTCAACACCT TCCTGCACTC CTACCGCGTC 
TTCACCACCG CCATCGTGGT CCTGGACAAG CTCATTACCA TCTACAAGAA GCCTATCAGT 
GCAATCCCTG CCAGGTGGCT GAGGTCGCTG GAGCTCCTGT TTGCCAGTGG CCAGAACAAT 
AAGCTTCTGT ATGGTGAACC CCCCAAGTCC CCGCGCGCCA CCCGCAAGTT CTCCTCGCCG 
CCACCCCTGT CCATCACCAA GACATCGTCG CCCAGCCGCC GGCGGAAGCT CTCCCTGAAC 
ATCCCCATCA TCACCGGCGG CAAGGCCCTG GACCTGGCCG CCCTCAGCTG CAACTCCAAT 
GGCTACACCA GCATGTACTC GGCCATGTCG CCCTTCAGCA AAGCCACGCT GGACACCAGC 
AAGCTCTATG TGTCCAGCAG CTTCACCAAC AAGATTCCAG ATGAGGGCGA TACGACCCCT 
GAGAAGCCCG AAGACCCTTC AGCGCTCAGC AAGCAGAGCT CAGAAGTCTC CGTGAGAGAG 
GAGTCAGATA CTGATCAAAA CCAGAGTGAT GATGGTGATA CTGAAACATC ACCAACTAAA 
TCTCCAACAA CACCCAAATC AGTCAAAAGC AAAAATTCTT CAGGGGCTAT GAGATACCCA 
ATGGTGAGAG AGAGCTCCCA GGGGACCCAC CTCCCGGGCA GCCCCCTCCT CACACAGCCT 
TCTCCAATGC CCCTGCTCTC ACATGCAAAG GGCCGCCTGC AGCCTGAACT CGCCACTATC 
CAGACCTTCA AAAGAAACCC AATAGGGTTT CCCCCAGACC AGAGGAATGG AGACAAGGAG 
TTTGTGATCC GCAGAGCAGC CACCAATCGT GTCTTGAATG TGCTCCGCCA CTGGGTGTCC 
AAGCACTCTC AGGACTTTGA GACCAACGAT GAGCTCAAAT GCAAGGTGAT CGGCTTCTTG 
GAAGAAGTCA TGCACGATCC GGAGCTCCTG ACCCAGGAGC GGAAGGCTGC AGCCAACATC 
ATCAGGACTC TGACCCAGGA GGACCCAGGT GACAACCAGA TCACACTGGA GGAGATCACG 
CAGATGGCTG AAGGCGTGAA GGCTGAGCCC TTTGAAAACC ACTCGGCCCT GGAGATCGCG 
GAGCAGCTGA CCCTGCTAGA CCACCTCGTC TTCAAGAAGA TTCCTTATGA GGAATTCTTC 
GGACAAGGAT GGATGAAACT GGAAAAGAAT GAAAGGACCC CTTATATCAT GAAAACCACT 
AAGCACTTCA ATGACATCAG TAACTTGATT GCTTCAGAAA TCATCCGCAA TGAGGACATC 
AACGCCAGGG TGAGCACCAT CGAGAAGTGG GTGGCCGTGG CTGACATATG CCGCTGCCTC 
CACAACTACA ATGCCGTGCT GGAGATCACC TCGTCCATGA ACCGCAGTGC AATCTTCCGG 
CTCAAAAAGA CGTGGCTCAA AGTCTCTAAG CAGACTAAAG CTTTGATTGA TAAGCTCCAA 
AAGCTTGTGT CATCTGAGGG CAGATTTAAG AATCTCAGAG AAGCTCTGAA AAATTGTGAC 
CCACCCTGTG TCCCTTACCT GGGGATGTAC CTCACCGACC TGGCCTTCAT CGAGGAGGGG 
ACGCCCAATT ACACGGAAGA CGGCCTGGTC AACTTCTCCA AGATGAGGAT GATATCCCAT 
ATTATCCGAG AGATTCGCCA GTTTCAACAA ACTGCCTACA AAATAGAGCA CCAAGCAAAG 
GTAACGCAAT ATTTACTGGA CCAATCTTTT GTAATGGATG AAGAAAGCCT CTACGAGTCT 
TCTCTCCGAA TAGAACCAAA ACTCCCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

RASGRF1 cDNA ORF clone, Nomascus leucogenys(northern white-cheeked gibbon)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム