EED cDNA ORF clone, Nipponia nippon(crested ibis)

Gene
Clones
gRNAs

Summary

Gene Symbol	EED
Entrez Gene ID	104017392
Full Name	embryonic ectoderm development
Gene Type	protein-coding
Organism	Nipponia nippon(crested ibis)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009470418.1	XP_009468693.1	polycomb protein EED

Pathways from BioSystems
Gene Ontology

The following EED gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EED cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONi170724	XM_009470418.1 Latest version!	Nipponia nippon embryonic ectoderm development (EED), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EED ( XM_009470418.1 ) cDNA ORF clone, Nipponia nippon(crested ibis) -> XP_009468693.1 Nipponia nippon embryonic ectoderm development (EED), mRNA.

Price & Availability↑

CloneID	ONi170724
Clone ID Related Accession (Same CDS sequence)	XM_009470418.1
Accession Version	XM_009470418.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-05
Organism	Nipponia nippon(crested ibis)
Product	polycomb protein EED
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009001908.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nipponia nippon Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 20% of CDS bases ##RefSeq-Attributes-END##

ATGCTCAGCC TGACATTCAG CCTGCGGCTG TGCAGCCGCT TTGCACCCAC AAAGCTGGAC 
GCCCGCCTGC CATCCGAGTA TTTACACGAG GCCGTGATTA GGAACGATTC ACTGCATGCC 
CTCAGCTATG AACCCCACTG CGAACTTGTC CCCACACCGC ATGGGCGACA GGGGAACGGC 
ATCGAAGGCG GGAATCCCCC CGACAGCCCC CTTGCTTTAG CAACCACACA GTGGCAGACT 
AGCTTTGCAG CTTCTTACAG AGTTGTTGGG CAACCAAAAT CAGTACATCT CTACCGGCTT 
CTGGCGATAC AGGATGATGC TGTTAGTATT GAAAGCGGTA CTAACACCGA ACGCCCTGAT 
ACACCAACAA ACACTCCTAA TGCACCGGGA AGGAAAAGCT GGGGGAAAGG AAAATGGAAG 
TCAAAGAAGT GCAAATATTC CTTCAAATGT GTAAATAGCC TAAAGGAGGA CCATGGTCAG 
CCTTTGTTTG GAGTCCAGTT TAACTGGCAC AGTAAAGAAG GTGATCCTCT TGTTTTTGCC 
ACTGTAGGCA GCAACAGAGT TACTTTATAT GAATGTCATT CCCAAGGAGA AATCCGTCTG 
TTGCAGTCGT ATGTGGATGC TGATGCAGAT GAAAATTTCT ATACCTGTGC GTGGACATAC 
GATAGCAATA CAAGCCATCC TCTTCTGGCT GTGGCAGGGT CCAGGGGTAT TATTAGGATA 
ATTAATCCTA TTACAATGCA GTGCATAAAG CACTACGTGG GCCATGGAAA TGCAATCAAT 
GAACTGAAAT TCCATCCAAG AGATCCAAAT CTCCTTTTAT CTGTAAGCAA AGATCATGCA 
TTGAGACTGT GGAACATCCA GACAGACACG CTGGTTGCAA TATTTGGAGG AGTAGAAGGG 
CACAGGGATG AGGTGTTAAG TGCAGATTAT GATCTTCTTG GTGAAAAAAT CATGTCCTGT 
GGGATGGATC ACTCTCTCAA GCTCTGGAGA ATTAATTCCA AGAGAATGAT AAACGCCATC 
AAAGAGTCTT ATGAGTACAA CCCAAACAAA ACCAACAGGC CTTTTATTTC CCAGAAAATT 
CACTTTCCCG ACTTTTCTAC AAGAGACATA CATAGAAACT ATGTTGACTG TGTACGATGG 
TTGGGAGATC TAATTCTTTC CAAGTCTTGT GAAAATGCCA TTGTGTGCTG GAAACCTGGC 
AAAATGGAAG ATGACATAGA TAAAATCAAG CCCAGTGAGT CAAACGTGAC CATACTTGGG 
AGATTTGATT ACAGCCAGTG TGATATATGG TACATGAGGT TTTCAATGGA TTTCTGGCAA 
AAGATGCTTG CTCTGGGCAA TCAGGTTGGC AAACTTTATG TTTGGGATTT AGAAATAGAG 
GATCCTCATA AAGCCAAGTG TACGACTCTT ACTCATCCTA AATGTGTTGC TGCCATTCGA 
CAAACCAGTT TTAGCAGGGA TAGTAGTATC CTTATAGCTG TGTGTGATGA TGCCAGTATC 
TGGCGCTGGG ACAGACTACG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009468693.1
CDS	1..1524
Translation	MLSLTFSLRLCSRFAPTKLDARLPSEYLHEAVIRNDSLHALSYEPHCELVPTPHGRQGNGIEGGNPPDSPLALATTQWQTSFAASYRVVGQPKSVHLYRLLAIQDDAVSIESGTNTERPDTPTNTPNAPGRKSWGKGKWKSKKCKYSFKCVNSLKEDHGQPLFGVQFNWHSKEGDPLVFATVGSNRVTLYECHSQGEIRLLQSYVDADADENFYTCAWTYDSNTSHPLLAVAGSRGIIRIINPITMQCIKHYVGHGNAINELKFHPRDPNLLLSVSKDHALRLWNIQTDTLVAIFGGVEGHRDEVLSADYDLLGEKIMSCGMDHSLKLWRINSKRMINAIKESYEYNPNKTNRPFISQKIHFPDFSTRDIHRNYVDCVRWLGDLILSKSCENAIVCWKPGKMEDDIDKIKPSESNVTILGRFDYSQCDIWYMRFSMDFWQKMLALGNQVGKLYVWDLEIEDPHKAKCTTLTHPKCVAAIRQTSFSRDSSILIAVCDDASIWRWDRLR

Target ORF information:

RefSeq Version	XM_009470418.1
Organism	Nipponia nippon(crested ibis)
Definition	Nipponia nippon embryonic ectoderm development (EED), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009470418.1

ORF Insert Sequence:

ATGCTCAGCC TGACATTCAG CCTGCGGCTG TGCAGCCGCT TTGCACCCAC AAAGCTGGAC 
GCCCGCCTGC CATCCGAGTA TTTACACGAG GCCGTGATTA GGAACGATTC ACTGCATGCC 
CTCAGCTATG AACCCCACTG CGAACTTGTC CCCACACCGC ATGGGCGACA GGGGAACGGC 
ATCGAAGGCG GGAATCCCCC CGACAGCCCC CTTGCTTTAG CAACCACACA GTGGCAGACT 
AGCTTTGCAG CTTCTTACAG AGTTGTTGGG CAACCAAAAT CAGTACATCT CTACCGGCTT 
CTGGCGATAC AGGATGATGC TGTTAGTATT GAAAGCGGTA CTAACACCGA ACGCCCTGAT 
ACACCAACAA ACACTCCTAA TGCACCGGGA AGGAAAAGCT GGGGGAAAGG AAAATGGAAG 
TCAAAGAAGT GCAAATATTC CTTCAAATGT GTAAATAGCC TAAAGGAGGA CCATGGTCAG 
CCTTTGTTTG GAGTCCAGTT TAACTGGCAC AGTAAAGAAG GTGATCCTCT TGTTTTTGCC 
ACTGTAGGCA GCAACAGAGT TACTTTATAT GAATGTCATT CCCAAGGAGA AATCCGTCTG 
TTGCAGTCGT ATGTGGATGC TGATGCAGAT GAAAATTTCT ATACCTGTGC GTGGACATAC 
GATAGCAATA CAAGCCATCC TCTTCTGGCT GTGGCAGGGT CCAGGGGTAT TATTAGGATA 
ATTAATCCTA TTACAATGCA GTGCATAAAG CACTACGTGG GCCATGGAAA TGCAATCAAT 
GAACTGAAAT TCCATCCAAG AGATCCAAAT CTCCTTTTAT CTGTAAGCAA AGATCATGCA 
TTGAGACTGT GGAACATCCA GACAGACACG CTGGTTGCAA TATTTGGAGG AGTAGAAGGG 
CACAGGGATG AGGTGTTAAG TGCAGATTAT GATCTTCTTG GTGAAAAAAT CATGTCCTGT 
GGGATGGATC ACTCTCTCAA GCTCTGGAGA ATTAATTCCA AGAGAATGAT AAACGCCATC 
AAAGAGTCTT ATGAGTACAA CCCAAACAAA ACCAACAGGC CTTTTATTTC CCAGAAAATT 
CACTTTCCCG ACTTTTCTAC AAGAGACATA CATAGAAACT ATGTTGACTG TGTACGATGG 
TTGGGAGATC TAATTCTTTC CAAGTCTTGT GAAAATGCCA TTGTGTGCTG GAAACCTGGC 
AAAATGGAAG ATGACATAGA TAAAATCAAG CCCAGTGAGT CAAACGTGAC CATACTTGGG 
AGATTTGATT ACAGCCAGTG TGATATATGG TACATGAGGT TTTCAATGGA TTTCTGGCAA 
AAGATGCTTG CTCTGGGCAA TCAGGTTGGC AAACTTTATG TTTGGGATTT AGAAATAGAG 
GATCCTCATA AAGCCAAGTG TACGACTCTT ACTCATCCTA AATGTGTTGC TGCCATTCGA 
CAAACCAGTT TTAGCAGGGA TAGTAGTATC CTTATAGCTG TGTGTGATGA TGCCAGTATC 
TGGCGCTGGG ACAGACTACG ATAA