LOC104223843 cDNA ORF clone, Nicotiana sylvestris(wood tobacco)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104223843
Entrez Gene ID	104223843
Full Name	protein POLLEN DEFECTIVE IN GUIDANCE 1
Gene Type	protein-coding
Organism	Nicotiana sylvestris(wood tobacco)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009775345.1	XP_009773647.1	protein POLLEN DEFECTIVE IN GUIDANCE 1

Pathways from BioSystems
Gene Ontology

The following LOC104223843 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104223843 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONi200548	XM_009775345.1 Latest version!	Nicotiana sylvestris protein POLLEN DEFECTIVE IN GUIDANCE 1 (LOC104223843), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104223843 ( XM_009775345.1 ) cDNA ORF clone, Nicotiana sylvestris(wood tobacco) -> XP_009773647.1 Nicotiana sylvestris protein POLLEN DEFECTIVE IN GUIDANCE 1 (LOC104223843), mRNA.

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CloneID	ONi200548
Clone ID Related Accession (Same CDS sequence)	XM_009775345.1
Accession Version	XM_009775345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Nicotiana sylvestris(wood tobacco)
Product	protein POLLEN DEFECTIVE IN GUIDANCE 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009564988.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nicotiana sylvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTGA GATCAACTGG ACGCAAGGTC TCATTTGACG TTCTCTCAAC TTCCATCCTA 
GACGACGATC AGGATTACGA TTCCTCCACC GCGACTCTCC CTCGATCCAA TTCCGATCCT 
CCTCCGCAGC TTCTGCCTAA TGATGACGCC ACAAGCCCAA CCGGAAACCG CAAGAGGAAG 
AAGAAAAAGA AGAAGCACAA GAGAATCGCC GAGCATTCCA CAATTTCCGA ATATTCCGTC 
ACGGACGAGC AGCTAGGCCG CTCATCCTCC GTCGGGGAAT TTAGCGGTTA CTGTTACAGT 
GTTGCTCAGA GCAGCAGTGT TGTGGTATGT GAGGAACCTG TTACACTGCC GAAGCCTGAG 
AGTAATTGTT CAGTGAGTTC AGTTTCTGGG CTTCCGTTTG GGGAGTTGAG GCAAAGAAAT 
GTTATGGTCA ATGGAGTGAC CGAAGAGAGC GTCGGATCAC CACAGATTAG TGAGAGACAA 
AATGAGAGTG TTGTTAAGGA ATTGGAATCG AGGTCAAATT CGAGAGTCGA AGTGAATGTG 
AATATGAATG GGGTTGCAGG GAGGAGTTTG GAGAAGGAAG CGTCTTTAGA TTGGAAGAAG 
TTGATGGCTG AAGATCCCAA TCAAACATTT CCGGTGGATA AGTCACCAAT GAAGTGCTTC 
ATGGAAGAGA TGTATGCGGG TAATTCCTTA AGGAGTACAG TTGCCCGCGG GGATGATAAA 
GAGCGGCAAA GAGTTTATGA TACCATATTT CGCTTACCAT GGCGATGTGA GCTGCTTATT 
AATGTTGGCT TTTTTGTCTG CTTTGATTCA TTCCTGTCAC TGCTAACTGT CATGCCAACG 
AGAATAATCA TGATCTGCTG GAGGTTTCTG AAAACAAGGC AGTTCAAGAA ACTTTCCGCA 
GTTGAGCTTT CTGATATTGG CTGTTGTGTT GCCCTGGCCA GTGGGGCGAT TTTGCTGCAA 
CAAACAGATA TCAGCCTAAT CTATCACATG ATTCGTGGTC AAGGGACAAT CAAGCTTTAT 
GTAGTTTACA ATGTGTTGGA GGTTTTTGAT AGACTATTCC AAAGCTTTAG TGGAGATGTG 
ATGCAGACTC TCTTTAATAC AGCAGAAGGG CTTGCAAATA GCTCAACGGA AAGTATGCAA 
TTGTGGATGA GAAGATTTAT TATGGATGAA GTTGTAGCTG TGGCTTCATC AATTCTTCAT 
TCTTTCATCT TGCTAGCTCA AGCTATCACT TTGTCAACCT GTATAGTTGC CCACAACAAT 
GCATTATTTG CTTTGCTTGT ATCAAACAAT TTTGCGGAAA TAAAGAGCAA TGTATTCAAA 
CGGTACAGCA AGGATAACGT TCACAATCTA GTGTATTATG ATTCAGTGGA GCGGTTCCAT 
ATTTCGGCAT TTCTCTTATT TGTGTTGGCT CAAAACCTTC TGGAGGCAGA TGGACCTTGG 
TTTGAAAGTT TTCTTTGTAA TGCATTTGTC GTCTACGTAA GTGAGATGAC AATTGATATA 
ATCAAGCATT CATTCATCGC AAAATTTAAC AATATCAAGC CGATTGCCTT TTCAGAGTTT 
CTTGAAGATC TCTGTAAGCA GACACTGAAT ATTCAGACGG ACAATATGAA GAATAACCTC 
ACTTTCGTCC CTTTGGCACC TGCTTGTGTG GTAATCCGGG TGCTGCGTCC TGTGTTTGCT 
GCTCATCTTC CCTATAATCC GCTTCCTTGG AGGCTCTTTT GGATTTTTCT TCTGTCTGCA 
ATGACATTTG TCATGCTTGC AAGCCTTAAA GTAATGATTA GCATTGGCCT AAAAAAGCAT 
GCGAGGTGGT ATATAAACAG ATGCCAGAGG AGAAAACTTC ATAGCGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009773647.1
CDS	609..2459
Translation	MALRSTGRKVSFDVLSTSILDDDQDYDSSTATLPRSNSDPPPQLLPNDDATSPTGNRKRKKKKKKHKRIAEHSTISEYSVTDEQLGRSSSVGEFSGYCYSVAQSSSVVVCEEPVTLPKPESNCSVSSVSGLPFGELRQRNVMVNGVTEESVGSPQISERQNESVVKELESRSNSRVEVNVNMNGVAGRSLEKEASLDWKKLMAEDPNQTFPVDKSPMKCFMEEMYAGNSLRSTVARGDDKERQRVYDTIFRLPWRCELLINVGFFVCFDSFLSLLTVMPTRIIMICWRFLKTRQFKKLSAVELSDIGCCVALASGAILLQQTDISLIYHMIRGQGTIKLYVVYNVLEVFDRLFQSFSGDVMQTLFNTAEGLANSSTESMQLWMRRFIMDEVVAVASSILHSFILLAQAITLSTCIVAHNNALFALLVSNNFAEIKSNVFKRYSKDNVHNLVYYDSVERFHISAFLLFVLAQNLLEADGPWFESFLCNAFVVYVSEMTIDIIKHSFIAKFNNIKPIAFSEFLEDLCKQTLNIQTDNMKNNLTFVPLAPACVVIRVLRPVFAAHLPYNPLPWRLFWIFLLSAMTFVMLASLKVMISIGLKKHARWYINRCQRRKLHSD

Target ORF information:

RefSeq Version	XM_009775345.1
Organism	Nicotiana sylvestris(wood tobacco)
Definition	Nicotiana sylvestris protein POLLEN DEFECTIVE IN GUIDANCE 1 (LOC104223843), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009775345.1

ORF Insert Sequence:

ATGGCGTTGA GATCAACTGG ACGCAAGGTC TCATTTGACG TTCTCTCAAC TTCCATCCTA 
GACGACGATC AGGATTACGA TTCCTCCACC GCGACTCTCC CTCGATCCAA TTCCGATCCT 
CCTCCGCAGC TTCTGCCTAA TGATGACGCC ACAAGCCCAA CCGGAAACCG CAAGAGGAAG 
AAGAAAAAGA AGAAGCACAA GAGAATCGCC GAGCATTCCA CAATTTCCGA ATATTCCGTC 
ACGGACGAGC AGCTAGGCCG CTCATCCTCC GTCGGGGAAT TTAGCGGTTA CTGTTACAGT 
GTTGCTCAGA GCAGCAGTGT TGTGGTATGT GAGGAACCTG TTACACTGCC GAAGCCTGAG 
AGTAATTGTT CAGTGAGTTC AGTTTCTGGG CTTCCGTTTG GGGAGTTGAG GCAAAGAAAT 
GTTATGGTCA ATGGAGTGAC CGAAGAGAGC GTCGGATCAC CACAGATTAG TGAGAGACAA 
AATGAGAGTG TTGTTAAGGA ATTGGAATCG AGGTCAAATT CGAGAGTCGA AGTGAATGTG 
AATATGAATG GGGTTGCAGG GAGGAGTTTG GAGAAGGAAG CGTCTTTAGA TTGGAAGAAG 
TTGATGGCTG AAGATCCCAA TCAAACATTT CCGGTGGATA AGTCACCAAT GAAGTGCTTC 
ATGGAAGAGA TGTATGCGGG TAATTCCTTA AGGAGTACAG TTGCCCGCGG GGATGATAAA 
GAGCGGCAAA GAGTTTATGA TACCATATTT CGCTTACCAT GGCGATGTGA GCTGCTTATT 
AATGTTGGCT TTTTTGTCTG CTTTGATTCA TTCCTGTCAC TGCTAACTGT CATGCCAACG 
AGAATAATCA TGATCTGCTG GAGGTTTCTG AAAACAAGGC AGTTCAAGAA ACTTTCCGCA 
GTTGAGCTTT CTGATATTGG CTGTTGTGTT GCCCTGGCCA GTGGGGCGAT TTTGCTGCAA 
CAAACAGATA TCAGCCTAAT CTATCACATG ATTCGTGGTC AAGGGACAAT CAAGCTTTAT 
GTAGTTTACA ATGTGTTGGA GGTTTTTGAT AGACTATTCC AAAGCTTTAG TGGAGATGTG 
ATGCAGACTC TCTTTAATAC AGCAGAAGGG CTTGCAAATA GCTCAACGGA AAGTATGCAA 
TTGTGGATGA GAAGATTTAT TATGGATGAA GTTGTAGCTG TGGCTTCATC AATTCTTCAT 
TCTTTCATCT TGCTAGCTCA AGCTATCACT TTGTCAACCT GTATAGTTGC CCACAACAAT 
GCATTATTTG CTTTGCTTGT ATCAAACAAT TTTGCGGAAA TAAAGAGCAA TGTATTCAAA 
CGGTACAGCA AGGATAACGT TCACAATCTA GTGTATTATG ATTCAGTGGA GCGGTTCCAT 
ATTTCGGCAT TTCTCTTATT TGTGTTGGCT CAAAACCTTC TGGAGGCAGA TGGACCTTGG 
TTTGAAAGTT TTCTTTGTAA TGCATTTGTC GTCTACGTAA GTGAGATGAC AATTGATATA 
ATCAAGCATT CATTCATCGC AAAATTTAAC AATATCAAGC CGATTGCCTT TTCAGAGTTT 
CTTGAAGATC TCTGTAAGCA GACACTGAAT ATTCAGACGG ACAATATGAA GAATAACCTC 
ACTTTCGTCC CTTTGGCACC TGCTTGTGTG GTAATCCGGG TGCTGCGTCC TGTGTTTGCT 
GCTCATCTTC CCTATAATCC GCTTCCTTGG AGGCTCTTTT GGATTTTTCT TCTGTCTGCA 
ATGACATTTG TCATGCTTGC AAGCCTTAAA GTAATGATTA GCATTGGCCT AAAAAAGCAT 
GCGAGGTGGT ATATAAACAG ATGCCAGAGG AGAAAACTTC ATAGCGACTA A