NECAME_11934 cDNA ORF clone, Necator americanus

Gene
Clones
gRNAs

Summary

Gene Symbol	NECAME_11934
Entrez Gene ID	25351962
Full Name	putative septum site-determining protein MinC
Gene Type	protein-coding
Organism	Necator americanus

mRNA and Protein(s)

mRNA	Protein	Name
XM_013442861.1	XP_013298315.1	putative septum site-determining protein MinC

Pathways from BioSystems
Gene Ontology

The following NECAME_11934 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NECAME_11934 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe127139	XM_013442861.1 Latest version!	Necator americanus putative septum site-determining protein MinC mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NECAME_11934 ( XM_013442861.1 ) cDNA ORF clone, Necator americanus -> XP_013298315.1 Necator americanus putative septum site-determining protein MinC mRNA.

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CloneID	ONe127139
Clone ID Related Accession (Same CDS sequence)	XM_013442861.1
Accession Version	XM_013442861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-11
Organism	Necator americanus
Product	putative septum site-determining protein MinC
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_013559683). COMPLETENESS: incomplete on both ends.

ATGGTGCAAG CAGGCGGCCA TCGACGTCCG TGGGCAGAGG CAGCCAGCAG TGCTTTTGCT 
GTTGCGTCAG CGCTTCCCTC CTCGATGAAT AGCGAACAGA TAGCCGACTT ATTCGAGAAG 
ACTGGGGAGA CATTTCTGAT TGCTGCTGCA CGTACCAACA ATGTGCATGC CGTCAAAAAT 
CTTGCAGCGG ATCCAGACTT GCTGGATGAA ACAGACAATG AAGGGTGGTC CGCTTTACTT 
AACGCCTCCC ACAAAGGTCA CGTGGATGTT GTTAGGATTC TTTTGGAAGC TGGAGCTTCG 
GTAGATCTAC CAGATATGAT GGGATGGAGT CCGTTAATGT GGGCTGTATA CAAATGTCAT 
CCAGAAATTG TTGATATGCT TCTCAACTAT GGCGCCCACG TAAATTTAAT AGATGAAGAA 
GATGGATTGA CACCACTCAT TGTTGCGTCC GGTCGAGGTT TCACCTCTGT TGTTGAAAAG 
TTATTAGCAG CAGATGCACA GGTTAATGCA TGCGATAAAT TTGGTAGCAC TGCTTTAATA 
TGGGCGGCTA GGAAAGGTCA TCTTCCAATT GTTCAAATGC TGTTAAACGC TGGTGCTGAA 
GTGGATGCTG TTGGAATGGG TTCTTCAACG GCTCTTATGT TGGCAACCAA AGGTAATTAC 
CTCACAGTTG TTGATTTGCT GCTAACTCGG GAACCAAATG TGAACGTCGT GGATCAGAAT 
GGATTGTCAG CATTAGGAAT CGCTGCACGA GAAGGCTATG CAGACATTTG TGAATCTCTA 
ATTAATTCTG GTGCTTTCGT GAATCAAAGT GATAAATATG GGAATTGGAT CTTAATTAGC 
GCAGTACGAA GTGGAAACGC TAATATAGTT CGACTGCTTC TAGAAAAATT TGCTGATGTA 
AACGCAAGAG ATTCTGAGAA CAGAACTGCT CTACATCTGG CCATCGACAA GTCCTTCATG 
GACATTGTAT ATATTATCCT GGAGAGGAAA CCGAACCTAG AGTTAAAGAA CAGGGATGGC 
GAAACTCCTC TTCTCCGTGC TGTCAAAAGT CGTCATGTGG CGCTTTGTGA TCTCCTTGTG 
AAGTCGGGTG CTAAAATTTC CGCCGCCGAT AATAATGGGG ACAACGCTCT TCATTTGGCG 
CTAAGAGCTA GAAGTCGACG ACTCACACAA ACGTTGTTAG CGAACCCCTC CGATTCCCGC 
CTTCTCTATC GACCTAACCG CTTGGGACAA ACTCCGTATT CCATTGACAA TGCGAATCCA 
CAACCTATTC TACCTCTTAT ATTCGGTCCC ATAGATGCCG AACAACATAT GGATACTATG 
TTAGGATATG ATGTGTACAG CAATGTACTT GCTGACATAG GTTTTCTTTA CTACGGAAGC 
GAAATGCGTG GATGGTCAGC CCCAGGTCGC GTTGCAAACT CTCTTTCGCG ATTCATCTAT 
CGAATCCGTC TTTTATGGAA CGTGGCAACT TTGTATGCAC CTATGCATTC AGAAAATGAC 
ATTGCGAGCA ATCCTGTGAG TTTTTTGTTC GCTGATTATC ATCGTCTTTC TTCTATTGGT 
GGTGAACAAG CGCTGGCAAA AATCGTCGCA ACGTTATTCG AGTCTGCTGA AACACACTTT 
GGAGTGCTGC CCGTTCGACT ATTTTGCTCA CTGAGAACAG CATATCCAGG ACAATACGGT 
TCTTTGCGGA GACACTGTGG AGTCCCAGTT GTGTTGCTTG TCGGAGTTGC TGTGTGCATG 
CTGCTCTCTG CTGAAGTGTT CATGATAATT TGGTTGTTCA GCGATAGAGA TACGAGTTCT 
GGAGCCCTAC TTGTTGCTTC GATTGCGTTC ATGGTAGTGT TCCTTATGCT AACTATCTAT 
CCTATTATTA TTTTGTTGCG ATATGGTTAT ACTAACGTCC CTCGAAAACA TGTTAACGAA 
GCAGCAAGAT CTGTACACAA GCTACGATTT GAAGGATTAA TGCAAAAGCT GCAAACTGAG 
GTGGACATCT TGGCGGACAT GATTAGATCG TTGGATGCAT TCACGAGAAG TCAAACGCGG 
CTCGTAGTTG TTGTGGATGG TCTCGATAAC TGTGAACAGG AGCGTATGGT TCAAACACTG 
GACGCGCTGG AACTTTTGTT TTCTGCACGG AAACATCGCC CGTTCATTAC TATTATCGCA 
GTTGATCCAC ACATTATTGT ATCTGCCATA AATCACAACA TGCATTCTGC GCTCACAGGA 
ACTGAACTTA CTGGTCATGA CTATCTGAAA AACATTGTTA ATATGCCTCT TTATCTTCAC 
AATTCTGCCC TTCGTCAATT GCAGAATAAG TTGAAAGACA AACGAGAATC ATTAGCGGAC 
TGGAAAGAGC GCTATCGCCG TCAAGATACA TTCCACGGTT CTCATTTATC ATTGGCCGAT 
GGACGACAAA GTCGTAAAGC AACAATAGTT GTTGGAACTC GCAGCATCGG TGAATCCTTG 
TTAAGTGACG ACTATTTCTC AAACATGAAT CCACGAGCTA TGAGGAGGAT TGTCAATGCG 
TTGACGCTAA CAGGCAGACT CATGCGAGCA TTTGAAATTG ATTTTTCATG GCTCTCGCTT 
GGCCACTGGG TTTCGTTACT GGAACAATGG CCCAGTCGAA TGTGTTGGCT TATTGATAGG 
GCCGTTGACA TAAATAATAA CGGGTTAACG CTTGCAGAGG TCTACCATCA ACTGAAGGAC 
CACATACCAA AAAAAGATTC ATTAATCGAA TTAGATAGGA ATCCAGACAA TTTTGAGGCA 
TTTCTCGATT CACCAACGAT TCCAAGCGCT GAGCAGCTAA CTGTAGGACA TGTTAAAAAG 
TTCGTGCCAT GCACTAGCAA CCTTGATCCA TACCTAAGGA AATTGATCAG AGAGCGTCGA 
CAAGGACTTG AAGATCCCGT GCAAGAACTT GGTTTCGGCA GTGTGGTTAT TCCTCCAAAT 
GCGAAATATC TGTTTGGGGA TGAAAAAGTG TGGAGTACAG TCTGCACTCC TCTGGTCGAA 
ATGAGGTTAG ATGATGTTTG TGCTCTACTG AAGAAGCTAG ACATTGCTTC GAATCGTTTG 
GACGCGATTG TTCGGAAATT CGAGCAATTG AATCTCGGAG GCTTAGTTCT GGCCTCTTGC 
TCGCTTCAGG ACTTGAAGGA TGCATTAGGG ATACCGCTTG GTGACTGGAC TATGGTGCGT 
CTTTTAGTGG AGACGTTGAA GGCGTTTGGC TCCAATGTTC CGGGAGTGAA GACGGATAAG 
AGAAGAGCTT TAACGCTCCC AGAAGAAGAT GAAGAAGGAT CGGAAGAGAG TGTAGATTTG 
ACGACAAGAC GTGGTACAAT CGTACAATCG CCGTCTGCAA TGATCACAGC TGAGGAGCGT 
AGGAGATCTA TGGCAATGCC TGAGGAAATA AACACTGATC ACAAGTGGTT GATGGAAAGC 
CTGTCTGGAA TGGATCTCAC GCAACCAGAA GGTGACCTGA ACTTGGCACC TTCATCTGCA 
GCTTCAGTAC GATTTGATGA TGCTGCTGAG GATGGGCTGG CTTCGGATGC TGACAGTACA 
GAGTCACGGT TTGGAAGCCG AGAAAACCTC CTTGATCCAG GACCGATATT GAGTCGTCAC 
TCTAGTAGTA CTGGAAGGGG CGATTTGATG AGACAGATCC ACAACGACTC CATTCATAGC 
ACGGAGGGTG TTATAAGTGC ACGATCATCA GTAGTGTCCG ATGACGACCA TCAGGTAACG 
ATAATGCGAC GGGACGGAAG TATCGTATCT GGAGAACGAC CACAATTGAC AAACATTTTT 
GGACGTGAAA ATGGCAATGC TAGACGAATA AAATGTGGGC ACTCTGCCAT GATGCCCATA 
AACGCTGTAG CAACACTTTA CTGGAGCGTA TCGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013298315.1
CDS	1..3879
Translation	MVQAGGHRRPWAEAASSAFAVASALPSSMNSEQIADLFEKTGETFLIAAARTNNVHAVKNLAADPDLLDETDNEGWSALLNASHKGHVDVVRILLEAGASVDLPDMMGWSPLMWAVYKCHPEIVDMLLNYGAHVNLIDEEDGLTPLIVASGRGFTSVVEKLLAADAQVNACDKFGSTALIWAARKGHLPIVQMLLNAGAEVDAVGMGSSTALMLATKGNYLTVVDLLLTREPNVNVVDQNGLSALGIAAREGYADICESLINSGAFVNQSDKYGNWILISAVRSGNANIVRLLLEKFADVNARDSENRTALHLAIDKSFMDIVYIILERKPNLELKNRDGETPLLRAVKSRHVALCDLLVKSGAKISAADNNGDNALHLALRARSRRLTQTLLANPSDSRLLYRPNRLGQTPYSIDNANPQPILPLIFGPIDAEQHMDTMLGYDVYSNVLADIGFLYYGSEMRGWSAPGRVANSLSRFIYRIRLLWNVATLYAPMHSENDIASNPVSFLFADYHRLSSIGGEQALAKIVATLFESAETHFGVLPVRLFCSLRTAYPGQYGSLRRHCGVPVVLLVGVAVCMLLSAEVFMIIWLFSDRDTSSGALLVASIAFMVVFLMLTIYPIIILLRYGYTNVPRKHVNEAARSVHKLRFEGLMQKLQTEVDILADMIRSLDAFTRSQTRLVVVVDGLDNCEQERMVQTLDALELLFSARKHRPFITIIAVDPHIIVSAINHNMHSALTGTELTGHDYLKNIVNMPLYLHNSALRQLQNKLKDKRESLADWKERYRRQDTFHGSHLSLADGRQSRKATIVVGTRSIGESLLSDDYFSNMNPRAMRRIVNALTLTGRLMRAFEIDFSWLSLGHWVSLLEQWPSRMCWLIDRAVDINNNGLTLAEVYHQLKDHIPKKDSLIELDRNPDNFEAFLDSPTIPSAEQLTVGHVKKFVPCTSNLDPYLRKLIRERRQGLEDPVQELGFGSVVIPPNAKYLFGDEKVWSTVCTPLVEMRLDDVCALLKKLDIASNRLDAIVRKFEQLNLGGLVLASCSLQDLKDALGIPLGDWTMVRLLVETLKAFGSNVPGVKTDKRRALTLPEEDEEGSEESVDLTTRRGTIVQSPSAMITAEERRRSMAMPEEINTDHKWLMESLSGMDLTQPEGDLNLAPSSAASVRFDDAAEDGLASDADSTESRFGSRENLLDPGPILSRHSSSTGRGDLMRQIHNDSIHSTEGVISARSSVVSDDDHQVTIMRRDGSIVSGERPQLTNIFGRENGNARRIKCGHSAMMPINAVATLYWSVSE

Target ORF information:

RefSeq Version	XM_013442861.1
Organism	Necator americanus
Definition	Necator americanus putative septum site-determining protein MinC mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013442861.1

ORF Insert Sequence:

ATGGTGCAAG CAGGCGGCCA TCGACGTCCG TGGGCAGAGG CAGCCAGCAG TGCTTTTGCT 
GTTGCGTCAG CGCTTCCCTC CTCGATGAAT AGCGAACAGA TAGCCGACTT ATTCGAGAAG 
ACTGGGGAGA CATTTCTGAT TGCTGCTGCA CGTACCAACA ATGTGCATGC CGTCAAAAAT 
CTTGCAGCGG ATCCAGACTT GCTGGATGAA ACAGACAATG AAGGGTGGTC CGCTTTACTT 
AACGCCTCCC ACAAAGGTCA CGTGGATGTT GTTAGGATTC TTTTGGAAGC TGGAGCTTCG 
GTAGATCTAC CAGATATGAT GGGATGGAGT CCGTTAATGT GGGCTGTATA CAAATGTCAT 
CCAGAAATTG TTGATATGCT TCTCAACTAT GGCGCCCACG TAAATTTAAT AGATGAAGAA 
GATGGATTGA CACCACTCAT TGTTGCGTCC GGTCGAGGTT TCACCTCTGT TGTTGAAAAG 
TTATTAGCAG CAGATGCACA GGTTAATGCA TGCGATAAAT TTGGTAGCAC TGCTTTAATA 
TGGGCGGCTA GGAAAGGTCA TCTTCCAATT GTTCAAATGC TGTTAAACGC TGGTGCTGAA 
GTGGATGCTG TTGGAATGGG TTCTTCAACG GCTCTTATGT TGGCAACCAA AGGTAATTAC 
CTCACAGTTG TTGATTTGCT GCTAACTCGG GAACCAAATG TGAACGTCGT GGATCAGAAT 
GGATTGTCAG CATTAGGAAT CGCTGCACGA GAAGGCTATG CAGACATTTG TGAATCTCTA 
ATTAATTCTG GTGCTTTCGT GAATCAAAGT GATAAATATG GGAATTGGAT CTTAATTAGC 
GCAGTACGAA GTGGAAACGC TAATATAGTT CGACTGCTTC TAGAAAAATT TGCTGATGTA 
AACGCAAGAG ATTCTGAGAA CAGAACTGCT CTACATCTGG CCATCGACAA GTCCTTCATG 
GACATTGTAT ATATTATCCT GGAGAGGAAA CCGAACCTAG AGTTAAAGAA CAGGGATGGC 
GAAACTCCTC TTCTCCGTGC TGTCAAAAGT CGTCATGTGG CGCTTTGTGA TCTCCTTGTG 
AAGTCGGGTG CTAAAATTTC CGCCGCCGAT AATAATGGGG ACAACGCTCT TCATTTGGCG 
CTAAGAGCTA GAAGTCGACG ACTCACACAA ACGTTGTTAG CGAACCCCTC CGATTCCCGC 
CTTCTCTATC GACCTAACCG CTTGGGACAA ACTCCGTATT CCATTGACAA TGCGAATCCA 
CAACCTATTC TACCTCTTAT ATTCGGTCCC ATAGATGCCG AACAACATAT GGATACTATG 
TTAGGATATG ATGTGTACAG CAATGTACTT GCTGACATAG GTTTTCTTTA CTACGGAAGC 
GAAATGCGTG GATGGTCAGC CCCAGGTCGC GTTGCAAACT CTCTTTCGCG ATTCATCTAT 
CGAATCCGTC TTTTATGGAA CGTGGCAACT TTGTATGCAC CTATGCATTC AGAAAATGAC 
ATTGCGAGCA ATCCTGTGAG TTTTTTGTTC GCTGATTATC ATCGTCTTTC TTCTATTGGT 
GGTGAACAAG CGCTGGCAAA AATCGTCGCA ACGTTATTCG AGTCTGCTGA AACACACTTT 
GGAGTGCTGC CCGTTCGACT ATTTTGCTCA CTGAGAACAG CATATCCAGG ACAATACGGT 
TCTTTGCGGA GACACTGTGG AGTCCCAGTT GTGTTGCTTG TCGGAGTTGC TGTGTGCATG 
CTGCTCTCTG CTGAAGTGTT CATGATAATT TGGTTGTTCA GCGATAGAGA TACGAGTTCT 
GGAGCCCTAC TTGTTGCTTC GATTGCGTTC ATGGTAGTGT TCCTTATGCT AACTATCTAT 
CCTATTATTA TTTTGTTGCG ATATGGTTAT ACTAACGTCC CTCGAAAACA TGTTAACGAA 
GCAGCAAGAT CTGTACACAA GCTACGATTT GAAGGATTAA TGCAAAAGCT GCAAACTGAG 
GTGGACATCT TGGCGGACAT GATTAGATCG TTGGATGCAT TCACGAGAAG TCAAACGCGG 
CTCGTAGTTG TTGTGGATGG TCTCGATAAC TGTGAACAGG AGCGTATGGT TCAAACACTG 
GACGCGCTGG AACTTTTGTT TTCTGCACGG AAACATCGCC CGTTCATTAC TATTATCGCA 
GTTGATCCAC ACATTATTGT ATCTGCCATA AATCACAACA TGCATTCTGC GCTCACAGGA 
ACTGAACTTA CTGGTCATGA CTATCTGAAA AACATTGTTA ATATGCCTCT TTATCTTCAC 
AATTCTGCCC TTCGTCAATT GCAGAATAAG TTGAAAGACA AACGAGAATC ATTAGCGGAC 
TGGAAAGAGC GCTATCGCCG TCAAGATACA TTCCACGGTT CTCATTTATC ATTGGCCGAT 
GGACGACAAA GTCGTAAAGC AACAATAGTT GTTGGAACTC GCAGCATCGG TGAATCCTTG 
TTAAGTGACG ACTATTTCTC AAACATGAAT CCACGAGCTA TGAGGAGGAT TGTCAATGCG 
TTGACGCTAA CAGGCAGACT CATGCGAGCA TTTGAAATTG ATTTTTCATG GCTCTCGCTT 
GGCCACTGGG TTTCGTTACT GGAACAATGG CCCAGTCGAA TGTGTTGGCT TATTGATAGG 
GCCGTTGACA TAAATAATAA CGGGTTAACG CTTGCAGAGG TCTACCATCA ACTGAAGGAC 
CACATACCAA AAAAAGATTC ATTAATCGAA TTAGATAGGA ATCCAGACAA TTTTGAGGCA 
TTTCTCGATT CACCAACGAT TCCAAGCGCT GAGCAGCTAA CTGTAGGACA TGTTAAAAAG 
TTCGTGCCAT GCACTAGCAA CCTTGATCCA TACCTAAGGA AATTGATCAG AGAGCGTCGA 
CAAGGACTTG AAGATCCCGT GCAAGAACTT GGTTTCGGCA GTGTGGTTAT TCCTCCAAAT 
GCGAAATATC TGTTTGGGGA TGAAAAAGTG TGGAGTACAG TCTGCACTCC TCTGGTCGAA 
ATGAGGTTAG ATGATGTTTG TGCTCTACTG AAGAAGCTAG ACATTGCTTC GAATCGTTTG 
GACGCGATTG TTCGGAAATT CGAGCAATTG AATCTCGGAG GCTTAGTTCT GGCCTCTTGC 
TCGCTTCAGG ACTTGAAGGA TGCATTAGGG ATACCGCTTG GTGACTGGAC TATGGTGCGT 
CTTTTAGTGG AGACGTTGAA GGCGTTTGGC TCCAATGTTC CGGGAGTGAA GACGGATAAG 
AGAAGAGCTT TAACGCTCCC AGAAGAAGAT GAAGAAGGAT CGGAAGAGAG TGTAGATTTG 
ACGACAAGAC GTGGTACAAT CGTACAATCG CCGTCTGCAA TGATCACAGC TGAGGAGCGT 
AGGAGATCTA TGGCAATGCC TGAGGAAATA AACACTGATC ACAAGTGGTT GATGGAAAGC 
CTGTCTGGAA TGGATCTCAC GCAACCAGAA GGTGACCTGA ACTTGGCACC TTCATCTGCA 
GCTTCAGTAC GATTTGATGA TGCTGCTGAG GATGGGCTGG CTTCGGATGC TGACAGTACA 
GAGTCACGGT TTGGAAGCCG AGAAAACCTC CTTGATCCAG GACCGATATT GAGTCGTCAC 
TCTAGTAGTA CTGGAAGGGG CGATTTGATG AGACAGATCC ACAACGACTC CATTCATAGC 
ACGGAGGGTG TTATAAGTGC ACGATCATCA GTAGTGTCCG ATGACGACCA TCAGGTAACG 
ATAATGCGAC GGGACGGAAG TATCGTATCT GGAGAACGAC CACAATTGAC AAACATTTTT 
GGACGTGAAA ATGGCAATGC TAGACGAATA AAATGTGGGC ACTCTGCCAT GATGCCCATA 
AACGCTGTAG CAACACTTTA CTGGAGCGTA TCGGAGTAA