Hrh3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hrh3
Entrez Gene ID	99296
Full Name	histamine receptor H3
Synonyms	AW049250,H3R,HH3R
General protein information	Preferred Names histamine receptor H3 Names histamine H3 receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 H4

mRNA and Protein(s)

mRNA	Protein	Name
XM_017319357.2	XP_017174846.1	histamine H3 receptor isoform X1
XM_017319357.1	XP_017174846.1	histamine H3 receptor isoform X1
NM_133849.3	NP_598610.1	histamine H3 receptor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HRH3	NP_009163.2
Canis lupus familiaris (dog)	HRH3	XP_005635315.1
Danio rerio (zebrafish)	LOC100334962	XP_003200969.1
Xenopus tropicalis (tropical clawed frog)	hrh3	XP_002939863.2
Mus musculus (house mouse)	Hrh3	NP_598610.1
Rattus norvegicus (Norway rat)	Hrh3	NP_445958.1
Gallus gallus (chicken)	HRH3	XP_004947140.1
	HRH3	NP_001028070.1
Bos taurus (cattle)	HRH3	XP_002692304.2
Danio rerio (zebrafish)	LOC100331077	XP_002667517.1

Gene Ontology
Bibliography

Related articles in PubMed

Histamine H3R receptor activation in the dorsal striatum triggers stereotypies in a mouse model of tic disorders.
Rapanelli M, Frick L, Pogorelov V, Ohtsu H, Bito H, Pittenger C
Translational psychiatry7(1)e1013(2017 01)

The Histamine H3 Receptor Differentially Modulates Mitogen-activated Protein Kinase (MAPK) and Akt Signaling in Striatonigral and Striatopallidal Neurons.
Rapanelli M, Frick LR, Horn KD, Schwarcz RC, Pogorelov V, Nairn AC, Pittenger C
The Journal of biological chemistry291(40)21042-21052(2016 Sep)

Histamine H3 Receptor Regulates Sensorimotor Gating and Dopaminergic Signaling in the Striatum.
Kononoff Vanhanen J, Nuutinen S, Tuominen M, Panula P
The Journal of pharmacology and experimental therapeutics357(2)264-72(2016 May)

On the Role of Histamine Receptors in the Regulation of Circadian Rhythms.
Rozov SV, Porkka-Heiskanen T, Panula P
PloS one10(12)e0144694(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The H3 receptor in the dorsal striatum is a contributor to repetitive behavioral pathology.
Title: Histamine H3R receptor activation in the dorsal striatum triggers stereotypies in a mouse model of tic disorders.

H3R activation with the agonist R-(-)-alpha-methylhistamine to produce a unique time- and cell type-dependent profile of molecular signaling events in the striatum.
Title: The Histamine H3 Receptor Differentially Modulates Mitogen-activated Protein Kinase (MAPK) and Akt Signaling in Striatonigral and Striatopallidal Neurons.

H3R is an important regulator of sensorimotor gating, and the lack of H3R significantly modifies striatal dopaminergic
Title: Histamine H3 Receptor Regulates Sensorimotor Gating and Dopaminergic Signaling in the Striatum.

These results suggest that H3 receptor may participate in the maintenance of the relaxed state and prevention of over-contraction in mature differentiated myotubes.
Title: Activation of histamine H3 receptor decreased cytoplasmic Ca(2+) imaging during electrical stimulation in the skeletal myotubes.

Data show that knockout of histamine receptor H3 (Hrh3 receptor) caused a substantial reduction of free-running activity rhythm amplitude.
Title: On the Role of Histamine Receptors in the Regulation of Circadian Rhythms.

The following Hrh3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hrh3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu13970	NM_133849.3 Latest version!	Mus musculus histamine receptor H3 (Hrh3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$265.30 $379.00
OMu80482	XM_017319357.2 Latest version!	Mus musculus histamine receptor H3 (Hrh3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OMu80482	XM_017319357.1	Mus musculus histamine receptor H3 (Hrh3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hrh3 ( XM_017319357.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174846.1 Mus musculus histamine receptor H3 (Hrh3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80482
Clone ID Related Accession (Same CDS sequence)	XM_017319357.2 , XM_017319357.1
Accession Version	XM_017319357.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	histamine H3 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TGTGGGTGCT GGCCTTCCTG CTGTATGGGC CTGCCATCCT GAGTTGGGAG 
TACCTGTCCG GTGGCAGCTC CATCCCCGAG GGCCACTGCT ATGCTGAGTT CTTCTACAAC 
TGGTACTTTC TCATCACGGC CTCCACCCTC GAGTTCTTCA CACCCTTCCT CAGCGTTACC 
TTCTTCAACC TCAGCATCTA CCTGAACATC CAGAGGCGCA CTCGTCTTCG GCTGGATGGG 
GGCCGAGAGG CTGGTCCAGA ACCCCCACCT GATGCCCAAC CCTCGCCACC TCCAGCTCCC 
CCCAGCTGCT GGGGCTGCTG GCCAAAGGGG CACGGGGAGG CCATGCCATT GCACAGGTAT 
GGGGTGGGTG AGGCAGGCCC TGGTGTTGAG ACTGGGGAGG CTGGCCTCGG GGGTGGCAGC 
GGTGGAGGCG CTGCTGCCTC GCCTACCTCC AGCTCCGGCA GCTCCTCAAG GGGCACTGAG 
AGGCCACGCT CACTCAAAAG GGGCTCCAAG CCATCAGCGT CTTCAGCGTC CTTGGAGAAG 
CGCATGAAGA TGGTATCCCA AAGCATCACC CAGCGCTTTC GGCTGTCGCG GGACAAGAAG 
GTAGCCAAGT CGCTGGCTAT CATCGTGAGC ATCTTTGGGC TCTGCTGGGC CCCGTACACA 
CTCCTCATGA TCATCCGGGC TGCTTGCCAT GGCCACTGCG TCCCCGACTA CTGGTACGAG 
ACGTCCTTCT GGCTTCTGTG GGCCAACTCG GCCGTCAACC CCGTCCTCTA CCCACTGTGC 
CACTACAGCT TCCGTAGAGC CTTCACCAAG CTCCTCTGCC CCCAGAAGCT CAAGGTCCAG 
CCCCATGGCT CCCTGGAGCA GTGCTGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174846.1
CDS	498..1370
Translation	MALVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFFYNWYFLITASTLEFFTPFLSVTFFNLSIYLNIQRRTRLRLDGGREAGPEPPPDAQPSPPPAPPSCWGCWPKGHGEAMPLHRYGVGEAGPGVETGEAGLGGGSGGGAAASPTSSSGSSSRGTERPRSLKRGSKPSASSASLEKRMKMVSQSITQRFRLSRDKKVAKSLAIIVSIFGLCWAPYTLLMIIRAACHGHCVPDYWYETSFWLLWANSAVNPVLYPLCHYSFRRAFTKLLCPQKLKVQPHGSLEQCWK

Target ORF information:

RefSeq Version	XM_017319357.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus histamine receptor H3 (Hrh3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319357.1

ORF Insert Sequence:

ATGGCACTGG TGTGGGTGCT GGCCTTCCTG CTGTATGGGC CTGCCATCCT GAGTTGGGAG 
TACCTGTCCG GTGGCAGCTC CATCCCCGAG GGCCACTGCT ATGCTGAGTT CTTCTACAAC 
TGGTACTTTC TCATCACGGC CTCCACCCTC GAGTTCTTCA CACCCTTCCT CAGCGTTACC 
TTCTTCAACC TCAGCATCTA CCTGAACATC CAGAGGCGCA CTCGTCTTCG GCTGGATGGG 
GGCCGAGAGG CTGGTCCAGA ACCCCCACCT GATGCCCAAC CCTCGCCACC TCCAGCTCCC 
CCCAGCTGCT GGGGCTGCTG GCCAAAGGGG CACGGGGAGG CCATGCCATT GCACAGGTAT 
GGGGTGGGTG AGGCAGGCCC TGGTGTTGAG ACTGGGGAGG CTGGCCTCGG GGGTGGCAGC 
GGTGGAGGCG CTGCTGCCTC GCCTACCTCC AGCTCCGGCA GCTCCTCAAG GGGCACTGAG 
AGGCCACGCT CACTCAAAAG GGGCTCCAAG CCATCAGCGT CTTCAGCGTC CTTGGAGAAG 
CGCATGAAGA TGGTATCCCA AAGCATCACC CAGCGCTTTC GGCTGTCGCG GGACAAGAAG 
GTAGCCAAGT CGCTGGCTAT CATCGTGAGC ATCTTTGGGC TCTGCTGGGC CCCGTACACA 
CTCCTCATGA TCATCCGGGC TGCTTGCCAT GGCCACTGCG TCCCCGACTA CTGGTACGAG 
ACGTCCTTCT GGCTTCTGTG GGCCAACTCG GCCGTCAACC CCGTCCTCTA CCCACTGTGC 
CACTACAGCT TCCGTAGAGC CTTCACCAAG CTCCTCTGCC CCCAGAAGCT CAAGGTCCAG 
CCCCATGGCT CCCTGGAGCA GTGCTGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrh3 ( NM_133849.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598610.1 Mus musculus histamine receptor H3 (Hrh3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13970
Clone ID Related Accession (Same CDS sequence)	NM_133849.3
Accession Version	NM_133849.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Mus musculus(house mouse)
Product	histamine H3 receptor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK140374.1, AY142145.1 and AW049250.1. On Mar 13, 2013 this sequence version replaced NM_133849.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK140374.1, AY044153.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGCG CGCCGCCCGA CGGGCTGATG AACGCGTCGG GCGCTCTGGC CGGAGAGGCG 
GCGGCTGCAG GCGGGGCGCG CGGCTTCTCG GCTGCCTGGA CCGCTGTCCT GGCTGCGCTC 
ATGGCGCTGC TCATCGTGGC CACAGTGCTG GGCAACGCGC TGGTCATGCT CGCCTTCGTG 
GCGGATTCGA GCCTCCGCAC CCAGAACAAC TTCTTTCTGC TCAACCTCGC CATCTCCGAC 
TTCCTCGTGG GTGCCTTCTG CATCCCATTG TATGTACCCT ATGTGCTGAC CGGCCGTTGG 
ACCTTTGGCC GGGGCCTCTG CAAGCTGTGG CTGGTGGTAG ACTACCTACT GTGTGCCTCC 
TCAGTCTTCA ACATCGTGCT GATCAGCTAT GACCGATTCC TGTCAGTCAC TCGAGCTGTC 
TCCTACCGGG CCCAGCAGGG GGACACAAGA CGGGCTGTTC GGAAGATGGC ACTGGTGTGG 
GTGCTGGCCT TCCTGCTGTA TGGGCCTGCC ATCCTGAGTT GGGAGTACCT GTCCGGTGGC 
AGCTCCATCC CCGAGGGCCA CTGCTATGCT GAGTTCTTCT ACAACTGGTA CTTTCTCATC 
ACGGCCTCCA CCCTCGAGTT CTTCACACCC TTCCTCAGCG TTACCTTCTT CAACCTCAGC 
ATCTACCTGA ACATCCAGAG GCGCACTCGT CTTCGGCTGG ATGGGGGCCG AGAGGCTGGT 
CCAGAACCCC CACCTGATGC CCAACCCTCG CCACCTCCAG CTCCCCCCAG CTGCTGGGGC 
TGCTGGCCAA AGGGGCACGG GGAGGCCATG CCATTGCACA GGTATGGGGT GGGTGAGGCA 
GGCCCTGGTG TTGAGACTGG GGAGGCTGGC CTCGGGGGTG GCAGCGGTGG AGGCGCTGCT 
GCCTCGCCTA CCTCCAGCTC CGGCAGCTCC TCAAGGGGCA CTGAGAGGCC ACGCTCACTC 
AAAAGGGGCT CCAAGCCATC AGCGTCTTCA GCGTCCTTGG AGAAGCGCAT GAAGATGGTA 
TCCCAAAGCA TCACCCAGCG CTTTCGGCTG TCGCGGGACA AGAAGGTAGC CAAGTCGCTG 
GCTATCATCG TGAGCATCTT TGGGCTCTGC TGGGCCCCGT ACACACTCCT CATGATCATC 
CGGGCTGCTT GCCATGGCCA CTGCGTCCCC GACTACTGGT ACGAGACGTC CTTCTGGCTT 
CTGTGGGCCA ACTCGGCCGT CAACCCCGTC CTCTACCCAC TGTGCCACTA CAGCTTCCGT 
AGAGCCTTCA CCAAGCTCCT CTGCCCCCAG AAGCTCAAGG TCCAGCCCCA TGGCTCCCTG 
GAGCAGTGCT GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598610.1
CDS	257..1594
Translation	MERAPPDGLMNASGALAGEAAAAGGARGFSAAWTAVLAALMALLIVATVLGNALVMLAFVADSSLRTQNNFFLLNLAISDFLVGAFCIPLYVPYVLTGRWTFGRGLCKLWLVVDYLLCASSVFNIVLISYDRFLSVTRAVSYRAQQGDTRRAVRKMALVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFFYNWYFLITASTLEFFTPFLSVTFFNLSIYLNIQRRTRLRLDGGREAGPEPPPDAQPSPPPAPPSCWGCWPKGHGEAMPLHRYGVGEAGPGVETGEAGLGGGSGGGAAASPTSSSGSSSRGTERPRSLKRGSKPSASSASLEKRMKMVSQSITQRFRLSRDKKVAKSLAIIVSIFGLCWAPYTLLMIIRAACHGHCVPDYWYETSFWLLWANSAVNPVLYPLCHYSFRRAFTKLLCPQKLKVQPHGSLEQCWK

Target ORF information:

RefSeq Version	NM_133849.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus histamine receptor H3 (Hrh3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133849.3

ORF Insert Sequence:

ATGGAGCGCG CGCCGCCCGA CGGGCTGATG AACGCGTCGG GCGCTCTGGC CGGAGAGGCG 
GCGGCTGCAG GCGGGGCGCG CGGCTTCTCG GCTGCCTGGA CCGCTGTCCT GGCTGCGCTC 
ATGGCGCTGC TCATCGTGGC CACAGTGCTG GGCAACGCGC TGGTCATGCT CGCCTTCGTG 
GCGGATTCGA GCCTCCGCAC CCAGAACAAC TTCTTTCTGC TCAACCTCGC CATCTCCGAC 
TTCCTCGTGG GTGCCTTCTG CATCCCATTG TATGTACCCT ATGTGCTGAC CGGCCGTTGG 
ACCTTTGGCC GGGGCCTCTG CAAGCTGTGG CTGGTGGTAG ACTACCTACT GTGTGCCTCC 
TCAGTCTTCA ACATCGTGCT GATCAGCTAT GACCGATTCC TGTCAGTCAC TCGAGCTGTC 
TCCTACCGGG CCCAGCAGGG GGACACAAGA CGGGCTGTTC GGAAGATGGC ACTGGTGTGG 
GTGCTGGCCT TCCTGCTGTA TGGGCCTGCC ATCCTGAGTT GGGAGTACCT GTCCGGTGGC 
AGCTCCATCC CCGAGGGCCA CTGCTATGCT GAGTTCTTCT ACAACTGGTA CTTTCTCATC 
ACGGCCTCCA CCCTCGAGTT CTTCACACCC TTCCTCAGCG TTACCTTCTT CAACCTCAGC 
ATCTACCTGA ACATCCAGAG GCGCACTCGT CTTCGGCTGG ATGGGGGCCG AGAGGCTGGT 
CCAGAACCCC CACCTGATGC CCAACCCTCG CCACCTCCAG CTCCCCCCAG CTGCTGGGGC 
TGCTGGCCAA AGGGGCACGG GGAGGCCATG CCATTGCACA GGTATGGGGT GGGTGAGGCA 
GGCCCTGGTG TTGAGACTGG GGAGGCTGGC CTCGGGGGTG GCAGCGGTGG AGGCGCTGCT 
GCCTCGCCTA CCTCCAGCTC CGGCAGCTCC TCAAGGGGCA CTGAGAGGCC ACGCTCACTC 
AAAAGGGGCT CCAAGCCATC AGCGTCTTCA GCGTCCTTGG AGAAGCGCAT GAAGATGGTA 
TCCCAAAGCA TCACCCAGCG CTTTCGGCTG TCGCGGGACA AGAAGGTAGC CAAGTCGCTG 
GCTATCATCG TGAGCATCTT TGGGCTCTGC TGGGCCCCGT ACACACTCCT CATGATCATC 
CGGGCTGCTT GCCATGGCCA CTGCGTCCCC GACTACTGGT ACGAGACGTC CTTCTGGCTT 
CTGTGGGCCA ACTCGGCCGT CAACCCCGTC CTCTACCCAC TGTGCCACTA CAGCTTCCGT 
AGAGCCTTCA CCAAGCTCCT CTGCCCCCAG AAGCTCAAGG TCCAGCCCCA TGGCTCCCTG 
GAGCAGTGCT GGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hrh3 ( XM_017319357.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017174846.1 Mus musculus histamine receptor H3 (Hrh3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu80482
Clone ID Related Accession (Same CDS sequence)	XM_017319357.2 , XM_017319357.1
Accession Version	XM_017319357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	histamine H3 receptor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017319357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG TGTGGGTGCT GGCCTTCCTG CTGTATGGGC CTGCCATCCT GAGTTGGGAG 
TACCTGTCCG GTGGCAGCTC CATCCCCGAG GGCCACTGCT ATGCTGAGTT CTTCTACAAC 
TGGTACTTTC TCATCACGGC CTCCACCCTC GAGTTCTTCA CACCCTTCCT CAGCGTTACC 
TTCTTCAACC TCAGCATCTA CCTGAACATC CAGAGGCGCA CTCGTCTTCG GCTGGATGGG 
GGCCGAGAGG CTGGTCCAGA ACCCCCACCT GATGCCCAAC CCTCGCCACC TCCAGCTCCC 
CCCAGCTGCT GGGGCTGCTG GCCAAAGGGG CACGGGGAGG CCATGCCATT GCACAGGTAT 
GGGGTGGGTG AGGCAGGCCC TGGTGTTGAG ACTGGGGAGG CTGGCCTCGG GGGTGGCAGC 
GGTGGAGGCG CTGCTGCCTC GCCTACCTCC AGCTCCGGCA GCTCCTCAAG GGGCACTGAG 
AGGCCACGCT CACTCAAAAG GGGCTCCAAG CCATCAGCGT CTTCAGCGTC CTTGGAGAAG 
CGCATGAAGA TGGTATCCCA AAGCATCACC CAGCGCTTTC GGCTGTCGCG GGACAAGAAG 
GTAGCCAAGT CGCTGGCTAT CATCGTGAGC ATCTTTGGGC TCTGCTGGGC CCCGTACACA 
CTCCTCATGA TCATCCGGGC TGCTTGCCAT GGCCACTGCG TCCCCGACTA CTGGTACGAG 
ACGTCCTTCT GGCTTCTGTG GGCCAACTCG GCCGTCAACC CCGTCCTCTA CCCACTGTGC 
CACTACAGCT TCCGTAGAGC CTTCACCAAG CTCCTCTGCC CCCAGAAGCT CAAGGTCCAG 
CCCCATGGCT CCCTGGAGCA GTGCTGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017174846.1
CDS	987..1859
Translation	MALVWVLAFLLYGPAILSWEYLSGGSSIPEGHCYAEFFYNWYFLITASTLEFFTPFLSVTFFNLSIYLNIQRRTRLRLDGGREAGPEPPPDAQPSPPPAPPSCWGCWPKGHGEAMPLHRYGVGEAGPGVETGEAGLGGGSGGGAAASPTSSSGSSSRGTERPRSLKRGSKPSASSASLEKRMKMVSQSITQRFRLSRDKKVAKSLAIIVSIFGLCWAPYTLLMIIRAACHGHCVPDYWYETSFWLLWANSAVNPVLYPLCHYSFRRAFTKLLCPQKLKVQPHGSLEQCWK

Target ORF information:

RefSeq Version	XM_017319357.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus histamine receptor H3 (Hrh3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017319357.2

ORF Insert Sequence:

ATGGCACTGG TGTGGGTGCT GGCCTTCCTG CTGTATGGGC CTGCCATCCT GAGTTGGGAG 
TACCTGTCCG GTGGCAGCTC CATCCCCGAG GGCCACTGCT ATGCTGAGTT CTTCTACAAC 
TGGTACTTTC TCATCACGGC CTCCACCCTC GAGTTCTTCA CACCCTTCCT CAGCGTTACC 
TTCTTCAACC TCAGCATCTA CCTGAACATC CAGAGGCGCA CTCGTCTTCG GCTGGATGGG 
GGCCGAGAGG CTGGTCCAGA ACCCCCACCT GATGCCCAAC CCTCGCCACC TCCAGCTCCC 
CCCAGCTGCT GGGGCTGCTG GCCAAAGGGG CACGGGGAGG CCATGCCATT GCACAGGTAT 
GGGGTGGGTG AGGCAGGCCC TGGTGTTGAG ACTGGGGAGG CTGGCCTCGG GGGTGGCAGC 
GGTGGAGGCG CTGCTGCCTC GCCTACCTCC AGCTCCGGCA GCTCCTCAAG GGGCACTGAG 
AGGCCACGCT CACTCAAAAG GGGCTCCAAG CCATCAGCGT CTTCAGCGTC CTTGGAGAAG 
CGCATGAAGA TGGTATCCCA AAGCATCACC CAGCGCTTTC GGCTGTCGCG GGACAAGAAG 
GTAGCCAAGT CGCTGGCTAT CATCGTGAGC ATCTTTGGGC TCTGCTGGGC CCCGTACACA 
CTCCTCATGA TCATCCGGGC TGCTTGCCAT GGCCACTGCG TCCCCGACTA CTGGTACGAG 
ACGTCCTTCT GGCTTCTGTG GGCCAACTCG GCCGTCAACC CCGTCCTCTA CCCACTGTGC 
CACTACAGCT TCCGTAGAGC CTTCACCAAG CTCCTCTGCC CCCAGAAGCT CAAGGTCCAG 
CCCCATGGCT CCCTGGAGCA GTGCTGGAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histamine H3R receptor activation in the dorsal striatum triggers stereotypies in a mouse model of tic disorders.
Translational psychiatry7(1)e1013(2017 01)
Rapanelli M,Frick L,Pogorelov V,Ohtsu H,Bito H,Pittenger C

The Histamine H3 Receptor Differentially Modulates Mitogen-activated Protein Kinase (MAPK) and Akt Signaling in Striatonigral and Striatopallidal Neurons.
The Journal of biological chemistry291(40)21042-21052(2016 Sep)
Rapanelli M,Frick LR,Horn KD,Schwarcz RC,Pogorelov V,Nairn AC,Pittenger C

Histamine H3 Receptor Regulates Sensorimotor Gating and Dopaminergic Signaling in the Striatum.
The Journal of pharmacology and experimental therapeutics357(2)264-72(2016 May)
Kononoff Vanhanen J,Nuutinen S,Tuominen M,Panula P

On the Role of Histamine Receptors in the Regulation of Circadian Rhythms.
PloS one10(12)e0144694(2015)
Rozov SV,Porkka-Heiskanen T,Panula P