Hadha cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hadha
Entrez Gene ID	97212
Full Name	hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
Synonyms	C77020,Mtpa
General protein information	Preferred Names hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit Names trifunctional enzyme subunit alpha, mitochondrial TP-alpha
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_178878.2	NP_849209.1	trifunctional enzyme subunit alpha, mitochondrial precursor
NM_178878.3	NP_849209.1	trifunctional enzyme subunit alpha, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	HADHA	NP_000173.2
	HADHA	XP_001086472.1
Canis lupus familiaris (dog)	HADHA	XP_003639634.1
Gallus gallus (chicken)	HADHA	NP_990387.1
Danio rerio (zebrafish)	hadhab	NP_001082906.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007784	XP_317731.4
Pan troglodytes (chimpanzee)	HADHA	XP_515339.2
Mus musculus (house mouse)	Hadha	NP_849209.1
Rattus norvegicus (Norway rat)	Hadha	NP_570839.2
Danio rerio (zebrafish)	hadhaa	NP_001098746.1
Caenorhabditis elegans (roundworm)	T08B2.7	NP_491789.1
Caenorhabditis elegans (roundworm)	ech-1	NP_506810.1
Bos taurus (cattle)	HADHA	NP_776760.1
Drosophila melanogaster (fruit fly)	Mtpalpha	NP_609299.1
Xenopus tropicalis (tropical clawed frog)	hadha	NP_989224.1

Gene Ontology
Bibliography

Related articles in PubMed

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
Schatton D, Pla-Martin D, Marx MC, Hansen H, Mourier A, Nemazanyy I, Pessia A, Zentis P, Corona T, Kondylis V, Barth E, Schauss AC, Velagapudi V, Rugarli EI
The Journal of cell biology216(3)675-693(2017 Mar)

HADHA, the alpha subunit of the mitochondrial trifunctional protein, is involved in long-chain fatty acid-induced autophagy in intestinal epithelial cells.
Maeyashiki C, Oshima S, Otsubo K, Kobayashi M, Nibe Y, Matsuzawa Y, Onizawa M, Nemoto Y, Nagaishi T, Okamoto R, Tsuchiya K, Nakamura T, Watanabe M
Biochemical and biophysical research communications484(3)636-641(2017 03)

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
Zhou L, Dai H, Wu J, Zhou M, Yuan H, Du J, Yang L, Wu X, Xu H, Hua Y, Xu J, Zheng L, Shen B
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)

Differential reduction in cardiac and liver monolysocardiolipin acyltransferase-1 and reduction in cardiac and liver tetralinoleoyl-cardiolipin in the ?-subunit of trifunctional protein heterozygous knockout mice.
Mejia EM, Ibdah JA, Sparagna GC, Hatch GM
The Biochemical journal471(1)123-9(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggested that palmitic acid-induced autophagy supports the survival of IECs. Taken together, these results suggested that HADHA is involved in long-chain fatty acid-induced autophagy in IECs, thus providing new insights into the pathology of IBD and revealing novel therapeutic targets of IBD.
Title: HADHA, the alpha subunit of the mitochondrial trifunctional protein, is involved in long-chain fatty acid-induced autophagy in intestinal epithelial cells.

alphaTFP may modulate CL molecular species composition in murine heart and liver. In addition, L4-CL might not be an essential requirement for mitochondrial supercomplex assembly.
Title: Differential reduction in cardiac and liver monolysocardiolipin acyltransferase-1 and reduction in cardiac and liver tetralinoleoyl-cardiolipin in the ?-subunit of trifunctional protein heterozygous knockout mice.

nonstructural protein 5 (NS5) interacted with hydroxyacyl-CoA dehydrogenase alpha and beta subunits, two components of the mitochondrial trifunctional protein (MTP) involved in LCFA beta-oxidation
Title: Japanese encephalitis virus nonstructural protein NS5 interacts with mitochondrial trifunctional protein and impairs fatty acid ?-oxidation.

Heterozygosity for beta-oxidation defects predisposes to fatty live disease and insulin resistance in aging mice
Title: Mice heterozygous for a defect in mitochondrial trifunctional protein develop hepatic steatosis and insulin resistance.

The following Hadha gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hadha cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu14895	NM_178878.3 Latest version!	Mus musculus hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (Hadha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$447.30 $639.00
View Old Accession Versions >>
OMu14895	NM_178878.2	Mus musculus hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$447.30 $639.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hadha ( NM_178878.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_849209.1 Mus musculus hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), mRNA.

Price & Availability↑

CloneID	OMu14895
Clone ID Related Accession (Same CDS sequence)	NM_178878.2 , NM_178878.3
Accession Version	NM_178878.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	trifunctional enzyme subunit alpha, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AK029017.1. On Apr 6, 2007 this sequence version replaced NM_178878.1. ##Evidence-Data-START## Transcript exon combination :: AK029017.1, AK170683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGTGGCGT CCCGGGCGAT TGGCAGTCTC AGTCGCTTCT CTGCCTTCAG GATCCTACGC 
TCCAGAGGCT GCATTTGCCG CAGCTTTACA ACGTCTTCTG CTCTGCTGAC CAGAACCCAT 
ATTAATTATG GCGTCAAAGG GGATGTGGCA GTTATTCGGA TTAACTCACC CAATTCAAAG 
GTAAATACAT TGAATAAAGA AGTACAGTCA GAGTTCATAG AAGTCATGAA CGAAATCTGG 
GCCAACGACC AAATCAGGAG TGCTGTCCTT ATCTCATCAA AGCCTGGCTG CTTTGTCGCA 
GGGGCTGACA TCAACATGCT ATCCTCTTGT ACAACCCCCC AAGAAGCAAC ACGAATATCA 
CAGGAAGGTC AGAGAATGTT TGAGAAACTT GAAAAGTCCC CAAAGCCCGT TGTGGCCGCC 
ATCAGTGGAT CCTGCTTGGG AGGAGGACTT GAGCTTGCCA TAGCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT GTCCCTGAAG TGTTGCTGGG GATCTTACCA 
GGAGCCGGAG GTACCCAGAG ACTGCCCAAA ATGGTGGGTG TGCCTGCTGC ATTTGACATG 
ATGCTGACTG GCAGGAACAT TCGTGCAGAC AGAGCAAAGA AAATGGGACT GGTTGACCAG 
TTGGTGGAAC CGCTGGGACC AGGAATAAAA TCACCAGAAG AAAGGACAAT TGAATACCTA 
GAGGAAGTTG CAGTTAATTT TGCCAAAGGC CTGGCTGATC GGAAAGTCTC TGCAAAGCAG 
AGCAAAGGCC TTGTGGAAAA GCTGACAACG TATGCCATGA CTGTCCCATT TGTCAGACAA 
CAGGTTTACA AAACAGTTGA AGAAAAAGTG AAGAAGCAGA CTAAAGGCCT TTATCCTGCC 
CCTTTGAAAA TAATTGATGC TGTGAAGGCT GGACTTGAGC AAGGCAGTGA CGCTGGTTAT 
CTTGCTGAAT CGCAGAAATT TGGAGAGCTT GCATTGACCA AAGAATCGAA GGCCCTGATG 
GGGCTTTATA ACGGCCAGGT CCTGTGCAAG AAAAATAAAT TTGGAGCACC ACAGAAGAAC 
GTTCAGCAGC TAGCCATCCT TGGCGCAGGG CTGATGGGGG CTGGCATTGC CCAGGTCTCT 
GTGGATAAGG GACTGAAAAC TCTTCTTAAA GACACCACAG TGACGGGGCT GGGCCGGGGA 
CAGCAGCAAG TGTTCAAAGG GCTGAACGAC AAAGTGAAGA AGAAAGCTCT CACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGATT GGGCAGCTGG ATTACAAAGG CTTTGAGAAG 
GCCGACATGG TGATTGAGGC TGTGTTTGAG GACCTCGGTG TAAAGCACAA AGTGTTAAAG 
GAAGTGGAAA GCGTGACTCC AGAACACTGT ATCTTTGCCA GCAACACATC TGCTCTCCCA 
ATCAATCAAA TTGCTGCTGT CAGCAAAAGG CCTGAGAAGG TGATCGGCAT GCACTACTTC 
TCCCCCGTGG ACAAGATGCA GCTCCTAGAA ATCATCACCA CTGACAAAAC CTCCAAGGAC 
ACAACGGCCT CTGCCGTGGC CGTGGGGCTC AGGCAAGGGA AGGTCATCAT TGTGGTCAAG 
GACGGACCCG GCTTCTACAC CACCAGGTGT CTTGCACCCA TGATGTCTGA AGTCATGCGA 
ATCCTCCAGG AAGGAGTTGA CCCTAAGAAG CTGGACGCCT TGACCACAGG TTTCGGCTTC 
CCTGTGGGTG CTGCCACGCT GGCAGACGAA GTGGGTGTGG ATGTAGCACA GCACGTAGCA 
GAAGATCTAG GCAAAGCCTT TGGGGAGCGA TTTGGAGGTG GCAGCGTAGA ACTGCTGAAA 
CAGATGGTCT CCAAGGGCTT CTTAGGTCGC AAGTCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGCTCAA AGAATAAGAG TTTGAATTCT GAAATGGATA ATATCTTGGC AAATCTGAGG 
CTGCCTGCCA AGCCCGAGGT CTCCTCTGAT GAAGACGTCC AATACCGTGT GATAACAAGG 
TTCGTGAACG AGGCAGTCCT GTGCCTACAG GAAGGGATCC TGGCCACACC GGCAGAGGGA 
GACATCGGAG CTGTCTTTGG GCTTGGCTTT CCCCCTTGTC TCGGAGGGCC CTTCCGCTTT 
GTGGATCTGT ATGGTGCTCA GAAGGTAGTG GACCGGCTAC GGAAGTACGA GTCTGCCTAT 
GGAACACAGT TTACCCCATG CCAGCTGCTC CTCGACCACG CTAACAACTC TAGCAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849209.1
CDS	33..2324
Translation	MVASRAIGSLSRFSAFRILRSRGCICRSFTTSSALLTRTHINYGVKGDVAVIRINSPNSKVNTLNKEVQSEFIEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLSSCTTPQEATRISQEGQRMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVEPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLVEKLTTYAMTVPFVRQQVYKTVEEKVKKQTKGLYPAPLKIIDAVKAGLEQGSDAGYLAESQKFGELALTKESKALMGLYNGQVLCKKNKFGAPQKNVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLGVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLRQGKVIIVVKDGPGFYTTRCLAPMMSEVMRILQEGVDPKKLDALTTGFGFPVGAATLADEVGVDVAQHVAEDLGKAFGERFGGGSVELLKQMVSKGFLGRKSGKGFYIYQEGSKNKSLNSEMDNILANLRLPAKPEVSSDEDVQYRVITRFVNEAVLCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLLDHANNSSKKFYQ

Target ORF information:

RefSeq Version	NM_178878.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit (Hadha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178878.2

ORF Insert Sequence:

ATGGTGGCGT CCCGGGCGAT TGGCAGTCTC AGTCGCTTCT CTGCCTTCAG GATCCTACGC 
TCCAGAGGCT GCATTTGCCG CAGCTTTACA ACGTCTTCTG CTCTGCTGAC CAGAACCCAT 
ATTAATTATG GCGTCAAAGG GGATGTGGCA GTTATTCGGA TTAACTCACC CAATTCAAAG 
GTAAATACAT TGAATAAAGA AGTACAGTCA GAGTTCATAG AAGTCATGAA CGAAATCTGG 
GCCAACGACC AAATCAGGAG TGCTGTCCTT ATCTCATCAA AGCCTGGCTG CTTTGTCGCA 
GGGGCTGACA TCAACATGCT ATCCTCTTGT ACAACCCCCC AAGAAGCAAC ACGAATATCA 
CAGGAAGGTC AGAGAATGTT TGAGAAACTT GAAAAGTCCC CAAAGCCCGT TGTGGCCGCC 
ATCAGTGGAT CCTGCTTGGG AGGAGGACTT GAGCTTGCCA TAGCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT GTCCCTGAAG TGTTGCTGGG GATCTTACCA 
GGAGCCGGAG GTACCCAGAG ACTGCCCAAA ATGGTGGGTG TGCCTGCTGC ATTTGACATG 
ATGCTGACTG GCAGGAACAT TCGTGCAGAC AGAGCAAAGA AAATGGGACT GGTTGACCAG 
TTGGTGGAAC CGCTGGGACC AGGAATAAAA TCACCAGAAG AAAGGACAAT TGAATACCTA 
GAGGAAGTTG CAGTTAATTT TGCCAAAGGC CTGGCTGATC GGAAAGTCTC TGCAAAGCAG 
AGCAAAGGCC TTGTGGAAAA GCTGACAACG TATGCCATGA CTGTCCCATT TGTCAGACAA 
CAGGTTTACA AAACAGTTGA AGAAAAAGTG AAGAAGCAGA CTAAAGGCCT TTATCCTGCC 
CCTTTGAAAA TAATTGATGC TGTGAAGGCT GGACTTGAGC AAGGCAGTGA CGCTGGTTAT 
CTTGCTGAAT CGCAGAAATT TGGAGAGCTT GCATTGACCA AAGAATCGAA GGCCCTGATG 
GGGCTTTATA ACGGCCAGGT CCTGTGCAAG AAAAATAAAT TTGGAGCACC ACAGAAGAAC 
GTTCAGCAGC TAGCCATCCT TGGCGCAGGG CTGATGGGGG CTGGCATTGC CCAGGTCTCT 
GTGGATAAGG GACTGAAAAC TCTTCTTAAA GACACCACAG TGACGGGGCT GGGCCGGGGA 
CAGCAGCAAG TGTTCAAAGG GCTGAACGAC AAAGTGAAGA AGAAAGCTCT CACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGATT GGGCAGCTGG ATTACAAAGG CTTTGAGAAG 
GCCGACATGG TGATTGAGGC TGTGTTTGAG GACCTCGGTG TAAAGCACAA AGTGTTAAAG 
GAAGTGGAAA GCGTGACTCC AGAACACTGT ATCTTTGCCA GCAACACATC TGCTCTCCCA 
ATCAATCAAA TTGCTGCTGT CAGCAAAAGG CCTGAGAAGG TGATCGGCAT GCACTACTTC 
TCCCCCGTGG ACAAGATGCA GCTCCTAGAA ATCATCACCA CTGACAAAAC CTCCAAGGAC 
ACAACGGCCT CTGCCGTGGC CGTGGGGCTC AGGCAAGGGA AGGTCATCAT TGTGGTCAAG 
GACGGACCCG GCTTCTACAC CACCAGGTGT CTTGCACCCA TGATGTCTGA AGTCATGCGA 
ATCCTCCAGG AAGGAGTTGA CCCTAAGAAG CTGGACGCCT TGACCACAGG TTTCGGCTTC 
CCTGTGGGTG CTGCCACGCT GGCAGACGAA GTGGGTGTGG ATGTAGCACA GCACGTAGCA 
GAAGATCTAG GCAAAGCCTT TGGGGAGCGA TTTGGAGGTG GCAGCGTAGA ACTGCTGAAA 
CAGATGGTCT CCAAGGGCTT CTTAGGTCGC AAGTCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGCTCAA AGAATAAGAG TTTGAATTCT GAAATGGATA ATATCTTGGC AAATCTGAGG 
CTGCCTGCCA AGCCCGAGGT CTCCTCTGAT GAAGACGTCC AATACCGTGT GATAACAAGG 
TTCGTGAACG AGGCAGTCCT GTGCCTACAG GAAGGGATCC TGGCCACACC GGCAGAGGGA 
GACATCGGAG CTGTCTTTGG GCTTGGCTTT CCCCCTTGTC TCGGAGGGCC CTTCCGCTTT 
GTGGATCTGT ATGGTGCTCA GAAGGTAGTG GACCGGCTAC GGAAGTACGA GTCTGCCTAT 
GGAACACAGT TTACCCCATG CCAGCTGCTC CTCGACCACG CTAACAACTC TAGCAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hadha ( NM_178878.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_849209.1 Mus musculus hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (Hadha), mRNA.

Price & Availability↑

CloneID	OMu14895
Clone ID Related Accession (Same CDS sequence)	NM_178878.2 , NM_178878.3
Accession Version	NM_178878.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-14
Organism	Mus musculus(house mouse)
Product	trifunctional enzyme subunit alpha, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK029017.1. On May 18, 2018 this sequence version replaced NM_178878.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK029017.1, AK170683.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164135 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGGCGT CCCGGGCGAT TGGCAGTCTC AGTCGCTTCT CTGCCTTCAG GATCCTACGC 
TCCAGAGGCT GCATTTGCCG CAGCTTTACA ACGTCTTCTG CTCTGCTGAC CAGAACCCAT 
ATTAATTATG GCGTCAAAGG GGATGTGGCA GTTATTCGGA TTAACTCACC CAATTCAAAG 
GTAAATACAT TGAATAAAGA AGTACAGTCA GAGTTCATAG AAGTCATGAA CGAAATCTGG 
GCCAACGACC AAATCAGGAG TGCTGTCCTT ATCTCATCAA AGCCTGGCTG CTTTGTCGCA 
GGGGCTGACA TCAACATGCT ATCCTCTTGT ACAACCCCCC AAGAAGCAAC ACGAATATCA 
CAGGAAGGTC AGAGAATGTT TGAGAAACTT GAAAAGTCCC CAAAGCCCGT TGTGGCCGCC 
ATCAGTGGAT CCTGCTTGGG AGGAGGACTT GAGCTTGCCA TAGCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT GTCCCTGAAG TGTTGCTGGG GATCTTACCA 
GGAGCCGGAG GTACCCAGAG ACTGCCCAAA ATGGTGGGTG TGCCTGCTGC ATTTGACATG 
ATGCTGACTG GCAGGAACAT TCGTGCAGAC AGAGCAAAGA AAATGGGACT GGTTGACCAG 
TTGGTGGAAC CGCTGGGACC AGGAATAAAA TCACCAGAAG AAAGGACAAT TGAATACCTA 
GAGGAAGTTG CAGTTAATTT TGCCAAAGGC CTGGCTGATC GGAAAGTCTC TGCAAAGCAG 
AGCAAAGGCC TTGTGGAAAA GCTGACAACG TATGCCATGA CTGTCCCATT TGTCAGACAA 
CAGGTTTACA AAACAGTTGA AGAAAAAGTG AAGAAGCAGA CTAAAGGCCT TTATCCTGCC 
CCTTTGAAAA TAATTGATGC TGTGAAGGCT GGACTTGAGC AAGGCAGTGA CGCTGGTTAT 
CTTGCTGAAT CGCAGAAATT TGGAGAGCTT GCATTGACCA AAGAATCGAA GGCCCTGATG 
GGGCTTTATA ACGGCCAGGT CCTGTGCAAG AAAAATAAAT TTGGAGCACC ACAGAAGAAC 
GTTCAGCAGC TAGCCATCCT TGGCGCAGGG CTGATGGGGG CTGGCATTGC CCAGGTCTCT 
GTGGATAAGG GACTGAAAAC TCTTCTTAAA GACACCACAG TGACGGGGCT GGGCCGGGGA 
CAGCAGCAAG TGTTCAAAGG GCTGAACGAC AAAGTGAAGA AGAAAGCTCT CACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGATT GGGCAGCTGG ATTACAAAGG CTTTGAGAAG 
GCCGACATGG TGATTGAGGC TGTGTTTGAG GACCTCGGTG TAAAGCACAA AGTGTTAAAG 
GAAGTGGAAA GCGTGACTCC AGAACACTGT ATCTTTGCCA GCAACACATC TGCTCTCCCA 
ATCAATCAAA TTGCTGCTGT CAGCAAAAGG CCTGAGAAGG TGATCGGCAT GCACTACTTC 
TCCCCCGTGG ACAAGATGCA GCTCCTAGAA ATCATCACCA CTGACAAAAC CTCCAAGGAC 
ACAACGGCCT CTGCCGTGGC CGTGGGGCTC AGGCAAGGGA AGGTCATCAT TGTGGTCAAG 
GACGGACCCG GCTTCTACAC CACCAGGTGT CTTGCACCCA TGATGTCTGA AGTCATGCGA 
ATCCTCCAGG AAGGAGTTGA CCCTAAGAAG CTGGACGCCT TGACCACAGG TTTCGGCTTC 
CCTGTGGGTG CTGCCACGCT GGCAGACGAA GTGGGTGTGG ATGTAGCACA GCACGTAGCA 
GAAGATCTAG GCAAAGCCTT TGGGGAGCGA TTTGGAGGTG GCAGCGTAGA ACTGCTGAAA 
CAGATGGTCT CCAAGGGCTT CTTAGGTCGC AAGTCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGCTCAA AGAATAAGAG TTTGAATTCT GAAATGGATA ATATCTTGGC AAATCTGAGG 
CTGCCTGCCA AGCCCGAGGT CTCCTCTGAT GAAGACGTCC AATACCGTGT GATAACAAGG 
TTCGTGAACG AGGCAGTCCT GTGCCTACAG GAAGGGATCC TGGCCACACC GGCAGAGGGA 
GACATCGGAG CTGTCTTTGG GCTTGGCTTT CCCCCTTGTC TCGGAGGGCC CTTCCGCTTT 
GTGGATCTGT ATGGTGCTCA GAAGGTAGTG GACCGGCTAC GGAAGTACGA GTCTGCCTAT 
GGAACACAGT TTACCCCATG CCAGCTGCTC CTCGACCACG CTAACAACTC TAGCAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_849209.1
CDS	33..2324
Translation	MVASRAIGSLSRFSAFRILRSRGCICRSFTTSSALLTRTHINYGVKGDVAVIRINSPNSKVNTLNKEVQSEFIEVMNEIWANDQIRSAVLISSKPGCFVAGADINMLSSCTTPQEATRISQEGQRMFEKLEKSPKPVVAAISGSCLGGGLELAIACQYRIATKDRKTVLGVPEVLLGILPGAGGTQRLPKMVGVPAAFDMMLTGRNIRADRAKKMGLVDQLVEPLGPGIKSPEERTIEYLEEVAVNFAKGLADRKVSAKQSKGLVEKLTTYAMTVPFVRQQVYKTVEEKVKKQTKGLYPAPLKIIDAVKAGLEQGSDAGYLAESQKFGELALTKESKALMGLYNGQVLCKKNKFGAPQKNVQQLAILGAGLMGAGIAQVSVDKGLKTLLKDTTVTGLGRGQQQVFKGLNDKVKKKALTSFERDSIFSNLIGQLDYKGFEKADMVIEAVFEDLGVKHKVLKEVESVTPEHCIFASNTSALPINQIAAVSKRPEKVIGMHYFSPVDKMQLLEIITTDKTSKDTTASAVAVGLRQGKVIIVVKDGPGFYTTRCLAPMMSEVMRILQEGVDPKKLDALTTGFGFPVGAATLADEVGVDVAQHVAEDLGKAFGERFGGGSVELLKQMVSKGFLGRKSGKGFYIYQEGSKNKSLNSEMDNILANLRLPAKPEVSSDEDVQYRVITRFVNEAVLCLQEGILATPAEGDIGAVFGLGFPPCLGGPFRFVDLYGAQKVVDRLRKYESAYGTQFTPCQLLLDHANNSSKKFYQ

Target ORF information:

RefSeq Version	NM_178878.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha (Hadha), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178878.3

ORF Insert Sequence:

ATGGTGGCGT CCCGGGCGAT TGGCAGTCTC AGTCGCTTCT CTGCCTTCAG GATCCTACGC 
TCCAGAGGCT GCATTTGCCG CAGCTTTACA ACGTCTTCTG CTCTGCTGAC CAGAACCCAT 
ATTAATTATG GCGTCAAAGG GGATGTGGCA GTTATTCGGA TTAACTCACC CAATTCAAAG 
GTAAATACAT TGAATAAAGA AGTACAGTCA GAGTTCATAG AAGTCATGAA CGAAATCTGG 
GCCAACGACC AAATCAGGAG TGCTGTCCTT ATCTCATCAA AGCCTGGCTG CTTTGTCGCA 
GGGGCTGACA TCAACATGCT ATCCTCTTGT ACAACCCCCC AAGAAGCAAC ACGAATATCA 
CAGGAAGGTC AGAGAATGTT TGAGAAACTT GAAAAGTCCC CAAAGCCCGT TGTGGCCGCC 
ATCAGTGGAT CCTGCTTGGG AGGAGGACTT GAGCTTGCCA TAGCATGCCA ATACAGAATA 
GCAACAAAAG ACAGAAAAAC AGTATTAGGT GTCCCTGAAG TGTTGCTGGG GATCTTACCA 
GGAGCCGGAG GTACCCAGAG ACTGCCCAAA ATGGTGGGTG TGCCTGCTGC ATTTGACATG 
ATGCTGACTG GCAGGAACAT TCGTGCAGAC AGAGCAAAGA AAATGGGACT GGTTGACCAG 
TTGGTGGAAC CGCTGGGACC AGGAATAAAA TCACCAGAAG AAAGGACAAT TGAATACCTA 
GAGGAAGTTG CAGTTAATTT TGCCAAAGGC CTGGCTGATC GGAAAGTCTC TGCAAAGCAG 
AGCAAAGGCC TTGTGGAAAA GCTGACAACG TATGCCATGA CTGTCCCATT TGTCAGACAA 
CAGGTTTACA AAACAGTTGA AGAAAAAGTG AAGAAGCAGA CTAAAGGCCT TTATCCTGCC 
CCTTTGAAAA TAATTGATGC TGTGAAGGCT GGACTTGAGC AAGGCAGTGA CGCTGGTTAT 
CTTGCTGAAT CGCAGAAATT TGGAGAGCTT GCATTGACCA AAGAATCGAA GGCCCTGATG 
GGGCTTTATA ACGGCCAGGT CCTGTGCAAG AAAAATAAAT TTGGAGCACC ACAGAAGAAC 
GTTCAGCAGC TAGCCATCCT TGGCGCAGGG CTGATGGGGG CTGGCATTGC CCAGGTCTCT 
GTGGATAAGG GACTGAAAAC TCTTCTTAAA GACACCACAG TGACGGGGCT GGGCCGGGGA 
CAGCAGCAAG TGTTCAAAGG GCTGAACGAC AAAGTGAAGA AGAAAGCTCT CACATCATTT 
GAAAGGGATT CCATCTTCAG CAACTTGATT GGGCAGCTGG ATTACAAAGG CTTTGAGAAG 
GCCGACATGG TGATTGAGGC TGTGTTTGAG GACCTCGGTG TAAAGCACAA AGTGTTAAAG 
GAAGTGGAAA GCGTGACTCC AGAACACTGT ATCTTTGCCA GCAACACATC TGCTCTCCCA 
ATCAATCAAA TTGCTGCTGT CAGCAAAAGG CCTGAGAAGG TGATCGGCAT GCACTACTTC 
TCCCCCGTGG ACAAGATGCA GCTCCTAGAA ATCATCACCA CTGACAAAAC CTCCAAGGAC 
ACAACGGCCT CTGCCGTGGC CGTGGGGCTC AGGCAAGGGA AGGTCATCAT TGTGGTCAAG 
GACGGACCCG GCTTCTACAC CACCAGGTGT CTTGCACCCA TGATGTCTGA AGTCATGCGA 
ATCCTCCAGG AAGGAGTTGA CCCTAAGAAG CTGGACGCCT TGACCACAGG TTTCGGCTTC 
CCTGTGGGTG CTGCCACGCT GGCAGACGAA GTGGGTGTGG ATGTAGCACA GCACGTAGCA 
GAAGATCTAG GCAAAGCCTT TGGGGAGCGA TTTGGAGGTG GCAGCGTAGA ACTGCTGAAA 
CAGATGGTCT CCAAGGGCTT CTTAGGTCGC AAGTCTGGGA AGGGCTTTTA CATCTATCAG 
GAGGGCTCAA AGAATAAGAG TTTGAATTCT GAAATGGATA ATATCTTGGC AAATCTGAGG 
CTGCCTGCCA AGCCCGAGGT CTCCTCTGAT GAAGACGTCC AATACCGTGT GATAACAAGG 
TTCGTGAACG AGGCAGTCCT GTGCCTACAG GAAGGGATCC TGGCCACACC GGCAGAGGGA 
GACATCGGAG CTGTCTTTGG GCTTGGCTTT CCCCCTTGTC TCGGAGGGCC CTTCCGCTTT 
GTGGATCTGT ATGGTGCTCA GAAGGTAGTG GACCGGCTAC GGAAGTACGA GTCTGCCTAT 
GGAACACAGT TTACCCCATG CCAGCTGCTC CTCGACCACG CTAACAACTC TAGCAAGAAG 
TTCTACCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs.
The Journal of cell biology216(3)675-693(2017 Mar)
Schatton D,Pla-Martin D,Marx MC,Hansen H,Mourier A,Nemazanyy I,Pessia A,Zentis P,Corona T,Kondylis V,Barth E,Schauss AC,Velagapudi V,Rugarli EI

HADHA, the alpha subunit of the mitochondrial trifunctional protein, is involved in long-chain fatty acid-induced autophagy in intestinal epithelial cells.
Biochemical and biophysical research communications484(3)636-641(2017 03)
Maeyashiki C,Oshima S,Otsubo K,Kobayashi M,Nibe Y,Matsuzawa Y,Onizawa M,Nemoto Y,Nagaishi T,Okamoto R,Tsuchiya K,Nakamura T,Watanabe M

Role of FEN1 S187 phosphorylation in counteracting oxygen-induced stress and regulating postnatal heart development.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(1)132-147(2017 01)
Zhou L,Dai H,Wu J,Zhou M,Yuan H,Du J,Yang L,Wu X,Xu H,Hua Y,Xu J,Zheng L,Shen B

Differential reduction in cardiac and liver monolysocardiolipin acyltransferase-1 and reduction in cardiac and liver tetralinoleoyl-cardiolipin in the ?-subunit of trifunctional protein heterozygous knockout mice.
The Biochemical journal471(1)123-9(2015 Oct)
Mejia EM,Ibdah JA,Sparagna GC,Hatch GM