Usp8 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp8
Entrez Gene ID	84092
Full Name	ubiquitin specific peptidase 8
Synonyms	AI574262,AW557536,Ubpy,mKIAA0055
General protein information	Preferred Names ubiquitin specific peptidase 8 Names ubiquitin carboxyl-terminal hydrolase 8 deubiquitinating enzyme 8 putative deubiquitinating enzyme ubiquitin isopeptidase Y ubiquitin thioesterase 8 ubiquitin thiolesterase 8 ubiquitin-specific-processing protease 8
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_011239857.2	XP_011238159.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
XM_011239857.3	XP_011238159.1	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
NM_001252580.1	NP_001239509.1	ubiquitin carboxyl-terminal hydrolase 8 isoform 1
NM_019729.3	NP_062703.2	ubiquitin carboxyl-terminal hydrolase 8 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	USP8	NP_005145.3
Drosophila melanogaster (fruit fly)	Ubpy	NP_650948.2
Mus musculus (house mouse)	Usp8	NP_001239509.1
Rattus norvegicus (Norway rat)	Usp8	NP_001099972.1
Gallus gallus (chicken)	USP8	XP_003641901.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002900	XP_312004.5
Caenorhabditis elegans (roundworm)	E01B7.1	NP_507513.2
Pan troglodytes (chimpanzee)	USP8	XP_001168786.1
Canis lupus familiaris (dog)	USP8	XP_535474.1
Bos taurus (cattle)	USP8	NP_001069594.1
Danio rerio (zebrafish)	usp8	XP_693811.5
Xenopus tropicalis (tropical clawed frog)	usp8	XP_004912669.1
	USP8	XP_001114516.1

Gene Ontology
Bibliography

Related articles in PubMed

Dynamic of contribution of UBPy-sorted cargo to acrosome biogenesis: effects of its derailment in a mouse model of globozoospermia, the infertile Vps54 (L967Q) mutant.
Gioria M, Pasini ME, Berruti G
Cell and tissue research(2017 Mar)

The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells.
Dufner A, Kisser A, Niendorf S, Basters A, Reissig S, Sch?nle A, Aichem A, Kurz T, Schlosser A, Yablonski D, Groettrup M, Buch T, Waisman A, Schamel WW, Prinz M, Knobeloch KP
Nature immunology16(9)950-60(2015 Sep)

USP8/UBPy-regulated sorting and the development of sperm acrosome: the recruitment of MET.
Berruti G, Paiardi C
Reproduction (Cambridge, England)149(6)633-44(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the membrane receptor MET, described herein for the first time in spermatids, is a USP8/UBPy-target substrate is delivered to the acrosome.
Title: USP8/UBPy-regulated sorting and the development of sperm acrosome: the recruitment of MET.

in neuronal cells USP8 could be involved in endosomal trafficking, retrograde transport and synaptic plasticity. In disorders leading to neurodegeneration USP8 is upregulated and could influence the neuron-oligodendrocyte interactions.
Title: The ESCRT-deubiquitinating enzyme USP8 in the cervical spinal cord of wild-type and Vps54-recessive (wobbler) mutant mice.

a role for the USP8.STAM complex as a protective mechanism regulating early endosomal sorting of EGFR between pathways destined for lysosomal degradation and recycling.
Title: Regulation of epidermal growth factor receptor ubiquitination and trafficking by the USP8?STAM complex.

The spatio-temporal expression of mUBPy suggests that the enzyme may be involved in neuroregulatory processes during embryogenesis.
Title: Expression pattern of mUBPy in the brain and sensory organs of mouse during embryonic development.

USP8 was able to interact with spermatid endosomal-sorting complex required for transport-0 and microtubule structures; USP8 can directly link, via its MIT domain, the labeled vesicles/developing acrosome to microtubules.
Title: USP8, a regulator of endosomal sorting, is involved in mouse acrosome biogenesis through interaction with the spermatid ESCRT-0 complex and microtubules.

The following Usp8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu11564	NM_019729.3 Latest version!	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu71879	XM_011239857.3 Latest version!	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu05291	NM_001252580.1 Latest version!	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OMu71879	XM_011239857.2	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp8 ( XM_011239857.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238159.1 Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu71879
Clone ID Related Accession (Same CDS sequence)	XM_011239857.2 , XM_011239857.3
Accession Version	XM_011239857.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011239857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATATG TGGCAGTTTA TAATCTTATC AAAAAGAGAC CTGATTTCAA GCAACAGCAG 
GATTATTATC TTTCAATACT TGGACCTGCA AACATCAAAA AAGCTATTGA AGAAGCTGAA 
AGACTCTCCG AAAGCCTTAA ACTAAGATAC GAAGAGGCTG AAGTTCGGAA ACAACTTGAA 
GAAAAGGACA GACGGGAGGA AGAGCAGCTG CAGCAACAGA AAAGGCAGGA GATGGGGAGA 
GAGGACAGCG GTGCGGCAGC CAAACGCTCT GTGGAAAATT TACTGGATTC CAAAACCAAA 
ACCCAAAGGA TTAACGGCGA GAAGAGTGAA GGAGCTGCAG CTGCAGAGAG AGGAGCCATC 
ACAGCAAAGG AACTATATAC GATGATGATG GATAAAAACA CAAGCTTAAT TATAATGGAT 
GCTCGAAAAA TACAGGATTA TCAGCATTCC TGTATCTTGG ATTCTCTCAG TGTTCCTGAA 
GAAGCTATCA GTCCAGGAGT CACTGCTAGT TGGATTGAAG CAAACCTCTC AGATGATTCT 
AAAGACACAT GGAAAAAGAG GGGGAGTGTG GACTATGTGG TCCTTCTCGA CTGGTTTAGT 
TCAGCGAAAG ATTTGCTGCT TGGGACCACT CTACGGAGTC TGAAAGATGC ACTTTTCAAG 
TGGGAAAGTA AAACTGTCCT GCGCCATGAG CCTTTGGTGT TGGAGGGCGG CTATGAAAAC 
TGGCTGCTTT GCTACCCGCA GTTTACAACC AATGCTAAGG TCACTCCACC CCCTCGGAGC 
AGAGCTGAAG AGGTGTCTGT CTCATTGGAT TTTACTTATC CCTCATTGGA AGAACCAGTT 
CCTTCCAAAC TTCCTACCCA GATGCCACCT CCTCCTATAG AAACAAATGA AAAGGCACTG 
TTGGTAACTG ATCAAGATGA AAAGCTGAGA CTGTCAACCC AGCCAGCTCT AGCTGGACCT 
GGTGCGGCTC CCAGAGCTGA AGCCTCACCC ATAATTCAGC CAGCGCCTGC TACAAAGAGT 
GTTCCACAGG TTGATCGTAC GAAAAAACCG TCAGTCAAGT TGCCTGAAGA TCATAGAATA 
AAATCTGAAA ATACAGATCA GAGTGGAAGA GTTCTTTCTG ATCGATCCAC CAAGCCAGTA 
TTTCCCTCTC CAACCACCAT GTTAACAGAT GAAGAAAAGG CTCGTATTCA TCAAGAAACT 
GCCCTTCTTA TGGAAAAGAA TAAACAGGAG AAGGAACTTT GGGACAAGCA GCAGAAGGAA 
CAGAAAGAGA AGCTGAGAAG GGAGGAACAA GAGCGCAAAG CTGGAAAGAC ACAGGATGCA 
GATGAACGTG ACTCCACTGA GAATCAGCAC AAAGCAAAGG ATGGACAAGA GAAAAAAGAC 
AGCAAACAGA CCAAGACGGA AGACAGAGAG CTTTCAGCAG ACGGGGCCCA GGAAGCCACA 
GGAACACAAA GACAAAGTAA GAGTGAGCAT GAAGCTTCTG ATGCTAAGGT ACCTGTGGAA 
GGTAAAAGGT GTCCCACGTC AGAGGCGCAG AAGAGGCCAG CAGATGTGTC CCCTGCATCC 
GTGTCAGGAG AGCTGAATGC AGGCAAGGCT CAGCGAGAAC CTTTGACGAG AGCACGAAGT 
GAAGAAATGG GGAGAATTGT GCCGGGACTG CCTTTGGGCT GGGCCAAGTT TCTTGATCCA 
ATCACCGGGA CCTTTCGTTA CTACCATTCC CCCACAAACA CAGTTCATAT GTATCCACCT 
GAAATGGCTC CTTCGTCTGC ACCTCCTTCC ACCCCGCCCA CTCACAAAGT CAAGCCCCAG 
GTCCCTGCCG AGCGGGACAG GGAGCCATCG AAACTGAAGC GCTCCTACTC CTCACCAGAT 
ATCACTCAGG CCCTGCAGGA GGAGGAGAAG AGGAGGCCGG CAGTGACCCC GATGGTCAAC 
CGGGAAAACA AGCCACCATG TTACCCTAAA GCTGAGATTT CGAGGCTTTC TGCTTCTCAG 
ATTCGGAACC TCAATCCTGT ATTTGGAGGA TCAGGACCAG CTCTTACTGG ACTTCGTAAT 
TTGGGAAATA CTTGTTACAT GAACTCAATC TTGCAGTGCC TGTGTAATGC TCCACATCTG 
GCTGATTATT TCAACCGAAA CTGCTACCAG GATGATATCA ACAGGTCAAA TTTGTTGGGG 
CATAAAGGTG AAGTGGCAGA AGAATTTGGT ATAATCATGA AAGCACTGTG GACAGGACAG 
TATAGATACA TCAGTCCAAA GGACTTTAAA GTCACCATTG GTAAGATTAA TGACCAGTTT 
GCAGGCTCCA GCCAACAGGA TTCACAAGAG CTGCTTCTGT TCCTCATGGA TGGCCTGCAT 
GAGGATCTGA ATAAGGCTGA CAATCGGAAG AGGCACAAGG AAGAGAACAA CGAGCACCTG 
GATGACCTGC AGGCGGCCGA GCACGCCTGG CAGAAGCACA AGCAGCTCAA CGAGTCCATC 
ATCGTGGCCC TGTTCCAGGG CCAGTTCAAG TCCACAGTGC AGTGCCTCAC CTGCCGCAGG 
AGGTCGCGCA CCTTCGAGGC CTTCATGTAC TTGTCTTTGC CGCTAGCATC CACAAGTAAA 
TGTACTCTAC AGGACTGCCT TAGATTATTT TCCAAAGAAG AAAAGCTTAC AGATAACAAC 
AGATTTTACT GCAGCCATTG CCGAGCTCGG CGGGATTCTC TAAAGAAAAT AGAAATCTGG 
AAATTACCTC CTGTGCTGTT AGTGCACCTG AAACGATTTT CCTATGACGG CAGGTGGAAG 
CAGAAGCTGC AAACATCCGT GGATTTCCCA TTGGAAAATC TTGACCTGTC ACAGTACGTT 
ATTGGTCCAA AAAACAGCTT GAAGAAATAT AACTTGTTTT CTGTTTCAAA CCACTACGGC 
GGGCTAGACG GAGGCCACTA CACAGCCTAC TGTAAGAACG CGGCAAGGCA GCGCTGGTTT 
AAGTTTGATG ACCATGAAGT TTCTGATATC TCTGTGTCTT CTGTGAGGTC ATCAGCAGCT 
TATATCCTCT TTTATACTTC CCTGGGACCA CGCATAACTG ATGTAGCCAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238159.1
CDS	566..3619
Translation	MKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKTQRINGEKSEGAAAAERGAITAKELYTMMMDKNTSLIIMDARKIQDYQHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDALFKWESKTVLRHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRAEEVSVSLDFTYPSLEEPVPSKLPTQMPPPPIETNEKALLVTDQDEKLRLSTQPALAGPGAAPRAEASPIIQPAPATKSVPQVDRTKKPSVKLPEDHRIKSENTDQSGRVLSDRSTKPVFPSPTTMLTDEEKARIHQETALLMEKNKQEKELWDKQQKEQKEKLRREEQERKAGKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQSKSEHEASDAKVPVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTPMVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHKEENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRRRSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRITDVAT

Target ORF information:

RefSeq Version	XM_011239857.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239857.2

ORF Insert Sequence:

ATGAAATATG TGGCAGTTTA TAATCTTATC AAAAAGAGAC CTGATTTCAA GCAACAGCAG 
GATTATTATC TTTCAATACT TGGACCTGCA AACATCAAAA AAGCTATTGA AGAAGCTGAA 
AGACTCTCCG AAAGCCTTAA ACTAAGATAC GAAGAGGCTG AAGTTCGGAA ACAACTTGAA 
GAAAAGGACA GACGGGAGGA AGAGCAGCTG CAGCAACAGA AAAGGCAGGA GATGGGGAGA 
GAGGACAGCG GTGCGGCAGC CAAACGCTCT GTGGAAAATT TACTGGATTC CAAAACCAAA 
ACCCAAAGGA TTAACGGCGA GAAGAGTGAA GGAGCTGCAG CTGCAGAGAG AGGAGCCATC 
ACAGCAAAGG AACTATATAC GATGATGATG GATAAAAACA CAAGCTTAAT TATAATGGAT 
GCTCGAAAAA TACAGGATTA TCAGCATTCC TGTATCTTGG ATTCTCTCAG TGTTCCTGAA 
GAAGCTATCA GTCCAGGAGT CACTGCTAGT TGGATTGAAG CAAACCTCTC AGATGATTCT 
AAAGACACAT GGAAAAAGAG GGGGAGTGTG GACTATGTGG TCCTTCTCGA CTGGTTTAGT 
TCAGCGAAAG ATTTGCTGCT TGGGACCACT CTACGGAGTC TGAAAGATGC ACTTTTCAAG 
TGGGAAAGTA AAACTGTCCT GCGCCATGAG CCTTTGGTGT TGGAGGGCGG CTATGAAAAC 
TGGCTGCTTT GCTACCCGCA GTTTACAACC AATGCTAAGG TCACTCCACC CCCTCGGAGC 
AGAGCTGAAG AGGTGTCTGT CTCATTGGAT TTTACTTATC CCTCATTGGA AGAACCAGTT 
CCTTCCAAAC TTCCTACCCA GATGCCACCT CCTCCTATAG AAACAAATGA AAAGGCACTG 
TTGGTAACTG ATCAAGATGA AAAGCTGAGA CTGTCAACCC AGCCAGCTCT AGCTGGACCT 
GGTGCGGCTC CCAGAGCTGA AGCCTCACCC ATAATTCAGC CAGCGCCTGC TACAAAGAGT 
GTTCCACAGG TTGATCGTAC GAAAAAACCG TCAGTCAAGT TGCCTGAAGA TCATAGAATA 
AAATCTGAAA ATACAGATCA GAGTGGAAGA GTTCTTTCTG ATCGATCCAC CAAGCCAGTA 
TTTCCCTCTC CAACCACCAT GTTAACAGAT GAAGAAAAGG CTCGTATTCA TCAAGAAACT 
GCCCTTCTTA TGGAAAAGAA TAAACAGGAG AAGGAACTTT GGGACAAGCA GCAGAAGGAA 
CAGAAAGAGA AGCTGAGAAG GGAGGAACAA GAGCGCAAAG CTGGAAAGAC ACAGGATGCA 
GATGAACGTG ACTCCACTGA GAATCAGCAC AAAGCAAAGG ATGGACAAGA GAAAAAAGAC 
AGCAAACAGA CCAAGACGGA AGACAGAGAG CTTTCAGCAG ACGGGGCCCA GGAAGCCACA 
GGAACACAAA GACAAAGTAA GAGTGAGCAT GAAGCTTCTG ATGCTAAGGT ACCTGTGGAA 
GGTAAAAGGT GTCCCACGTC AGAGGCGCAG AAGAGGCCAG CAGATGTGTC CCCTGCATCC 
GTGTCAGGAG AGCTGAATGC AGGCAAGGCT CAGCGAGAAC CTTTGACGAG AGCACGAAGT 
GAAGAAATGG GGAGAATTGT GCCGGGACTG CCTTTGGGCT GGGCCAAGTT TCTTGATCCA 
ATCACCGGGA CCTTTCGTTA CTACCATTCC CCCACAAACA CAGTTCATAT GTATCCACCT 
GAAATGGCTC CTTCGTCTGC ACCTCCTTCC ACCCCGCCCA CTCACAAAGT CAAGCCCCAG 
GTCCCTGCCG AGCGGGACAG GGAGCCATCG AAACTGAAGC GCTCCTACTC CTCACCAGAT 
ATCACTCAGG CCCTGCAGGA GGAGGAGAAG AGGAGGCCGG CAGTGACCCC GATGGTCAAC 
CGGGAAAACA AGCCACCATG TTACCCTAAA GCTGAGATTT CGAGGCTTTC TGCTTCTCAG 
ATTCGGAACC TCAATCCTGT ATTTGGAGGA TCAGGACCAG CTCTTACTGG ACTTCGTAAT 
TTGGGAAATA CTTGTTACAT GAACTCAATC TTGCAGTGCC TGTGTAATGC TCCACATCTG 
GCTGATTATT TCAACCGAAA CTGCTACCAG GATGATATCA ACAGGTCAAA TTTGTTGGGG 
CATAAAGGTG AAGTGGCAGA AGAATTTGGT ATAATCATGA AAGCACTGTG GACAGGACAG 
TATAGATACA TCAGTCCAAA GGACTTTAAA GTCACCATTG GTAAGATTAA TGACCAGTTT 
GCAGGCTCCA GCCAACAGGA TTCACAAGAG CTGCTTCTGT TCCTCATGGA TGGCCTGCAT 
GAGGATCTGA ATAAGGCTGA CAATCGGAAG AGGCACAAGG AAGAGAACAA CGAGCACCTG 
GATGACCTGC AGGCGGCCGA GCACGCCTGG CAGAAGCACA AGCAGCTCAA CGAGTCCATC 
ATCGTGGCCC TGTTCCAGGG CCAGTTCAAG TCCACAGTGC AGTGCCTCAC CTGCCGCAGG 
AGGTCGCGCA CCTTCGAGGC CTTCATGTAC TTGTCTTTGC CGCTAGCATC CACAAGTAAA 
TGTACTCTAC AGGACTGCCT TAGATTATTT TCCAAAGAAG AAAAGCTTAC AGATAACAAC 
AGATTTTACT GCAGCCATTG CCGAGCTCGG CGGGATTCTC TAAAGAAAAT AGAAATCTGG 
AAATTACCTC CTGTGCTGTT AGTGCACCTG AAACGATTTT CCTATGACGG CAGGTGGAAG 
CAGAAGCTGC AAACATCCGT GGATTTCCCA TTGGAAAATC TTGACCTGTC ACAGTACGTT 
ATTGGTCCAA AAAACAGCTT GAAGAAATAT AACTTGTTTT CTGTTTCAAA CCACTACGGC 
GGGCTAGACG GAGGCCACTA CACAGCCTAC TGTAAGAACG CGGCAAGGCA GCGCTGGTTT 
AAGTTTGATG ACCATGAAGT TTCTGATATC TCTGTGTCTT CTGTGAGGTC ATCAGCAGCT 
TATATCCTCT TTTATACTTC CCTGGGACCA CGCATAACTG ATGTAGCCAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp8 ( NM_019729.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062703.2 Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu11564
Clone ID Related Accession (Same CDS sequence)	NM_019729.3
Accession Version	NM_019729.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY147904.1, BC050947.1 and BY639666.1. On Nov 19, 2011 this sequence version replaced NM_019729.2. Transcript Variant: This variant (2) uses an alternate splice site in the 5' coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.46147.1, BC050947.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT AAAAGACCTC 
AATAAGAAGA CCGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCG 
CAGAAGATCT TCAAGACAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCAGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ATTATTATCT TTCAATACTT GGACCTGCAA ACATCAAAAA AGCTATTGAA 
GAAGCTGAAA GACTCTCCGA AAGCCTTAAA CTAAGATACG AAGAGGCTGA AGTTCGGAAA 
CAACTTGAAG AAAAGGACAG ACGGGAGGAA GAGCAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGGAGAG AGGACAGCGG TGCGGCAGCC AAACGCTCTG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA CCCAAAGGAT TAACGGCGAG AAGAGTGAAG GAGCTGCAGC TGCAGAGAGA 
GGAGCCATCA CAGCAAAGGA ACTATATACG ATGATGATGG ATAAAAACAC AAGCTTAATT 
ATAATGGATG CTCGAAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGA TTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCTAGTT GGATTGAAGC AAACCTCTCA 
GATGATTCTA AAGACACATG GAAAAAGAGG GGGAGTGTGG ACTATGTGGT CCTTCTCGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTT GGGACCACTC TACGGAGTCT GAAAGATGCA 
CTTTTCAAGT GGGAAAGTAA AACTGTCCTG CGCCATGAGC CTTTGGTGTT GGAGGGCGGC 
TATGAAAACT GGCTGCTTTG CTACCCGCAG TTTACAACCA ATGCTAAGGT CACTCCACCC 
CCTCGGAGCA GAGCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCATTGGAA 
GAACCAGTTC CTTCCAAACT TCCTACCCAG ATGCCACCTC CTCCTATAGA AACAAATGAA 
AAGGCACTGT TGGTAACTGA TCAAGATGAA AAGCTGAGAC TGTCAACCCA GCCAGCTCTA 
GCTGGACCTG GTGCGGCTCC CAGAGCTGAA GCCTCACCCA TAATTCAGCC AGCGCCTGCT 
ACAAAGAGTG TTCCACAGGT TGATCGTACG AAAAAACCGT CAGTCAAGTT GCCTGAAGAT 
CATAGAATAA AATCTGAAAA TACAGATCAG AGTGGAAGAG TTCTTTCTGA TCGATCCACC 
AAGCCAGTAT TTCCCTCTCC AACCACCATG TTAACAGATG AAGAAAAGGC TCGTATTCAT 
CAAGAAACTG CCCTTCTTAT GGAAAAGAAT AAACAGGAGA AGGAACTTTG GGACAAGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACA 
CAGGATGCAG ATGAACGTGA CTCCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAAAAAGACA GCAAACAGAC CAAGACGGAA GACAGAGAGC TTTCAGCAGA CGGGGCCCAG 
GAAGCCACAG GAACACAAAG ACAAAGTAAG AGTGAGCATG AAGCTTCTGA TGCTAAGGTA 
CCTGTGGAAG GTAAAAGGTG TCCCACGTCA GAGGCGCAGA AGAGGCCAGC AGATGTGTCC 
CCTGCATCCG TGTCAGGAGA GCTGAATGCA GGCAAGGCTC AGCGAGAACC TTTGACGAGA 
GCACGAAGTG AAGAAATGGG GAGAATTGTG CCGGGACTGC CTTTGGGCTG GGCCAAGTTT 
CTTGATCCAA TCACCGGGAC CTTTCGTTAC TACCATTCCC CCACAAACAC AGTTCATATG 
TATCCACCTG AAATGGCTCC TTCGTCTGCA CCTCCTTCCA CCCCGCCCAC TCACAAAGTC 
AAGCCCCAGG TCCCTGCCGA GCGGGACAGG GAGCCATCGA AACTGAAGCG CTCCTACTCC 
TCACCAGATA TCACTCAGGC CCTGCAGGAG GAGGAGAAGA GGAGGCCGGC AGTGACCCCG 
ATGGTCAACC GGGAAAACAA GCCACCATGT TACCCTAAAG CTGAGATTTC GAGGCTTTCT 
GCTTCTCAGA TTCGGAACCT CAATCCTGTA TTTGGAGGAT CAGGACCAGC TCTTACTGGA 
CTTCGTAATT TGGGAAATAC TTGTTACATG AACTCAATCT TGCAGTGCCT GTGTAATGCT 
CCACATCTGG CTGATTATTT CAACCGAAAC TGCTACCAGG ATGATATCAA CAGGTCAAAT 
TTGTTGGGGC ATAAAGGTGA AGTGGCAGAA GAATTTGGTA TAATCATGAA AGCACTGTGG 
ACAGGACAGT ATAGATACAT CAGTCCAAAG GACTTTAAAG TCACCATTGG TAAGATTAAT 
GACCAGTTTG CAGGCTCCAG CCAACAGGAT TCACAAGAGC TGCTTCTGTT CCTCATGGAT 
GGCCTGCATG AGGATCTGAA TAAGGCTGAC AATCGGAAGA GGCACAAGGA AGAGAACAAC 
GAGCACCTGG ATGACCTGCA GGCGGCCGAG CACGCCTGGC AGAAGCACAA GCAGCTCAAC 
GAGTCCATCA TCGTGGCCCT GTTCCAGGGC CAGTTCAAGT CCACAGTGCA GTGCCTCACC 
TGCCGCAGGA GGTCGCGCAC CTTCGAGGCC TTCATGTACT TGTCTTTGCC GCTAGCATCC 
ACAAGTAAAT GTACTCTACA GGACTGCCTT AGATTATTTT CCAAAGAAGA AAAGCTTACA 
GATAACAACA GATTTTACTG CAGCCATTGC CGAGCTCGGC GGGATTCTCT AAAGAAAATA 
GAAATCTGGA AATTACCTCC TGTGCTGTTA GTGCACCTGA AACGATTTTC CTATGACGGC 
AGGTGGAAGC AGAAGCTGCA AACATCCGTG GATTTCCCAT TGGAAAATCT TGACCTGTCA 
CAGTACGTTA TTGGTCCAAA AAACAGCTTG AAGAAATATA ACTTGTTTTC TGTTTCAAAC 
CACTACGGCG GGCTAGACGG AGGCCACTAC ACAGCCTACT GTAAGAACGC GGCAAGGCAG 
CGCTGGTTTA AGTTTGATGA CCATGAAGTT TCTGATATCT CTGTGTCTTC TGTGAGGTCA 
TCAGCAGCTT ATATCCTCTT TTATACTTCC CTGGGACCAC GCATAACTGA TGTAGCCACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062703.2
CDS	227..3469
Translation	MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNYIHSAQKIFKTAEECRLDRDEERAYVLYMKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKTQRINGEKSEGAAAAERGAITAKELYTMMMDKNTSLIIMDARKIQDYQHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDALFKWESKTVLRHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRAEEVSVSLDFTYPSLEEPVPSKLPTQMPPPPIETNEKALLVTDQDEKLRLSTQPALAGPGAAPRAEASPIIQPAPATKSVPQVDRTKKPSVKLPEDHRIKSENTDQSGRVLSDRSTKPVFPSPTTMLTDEEKARIHQETALLMEKNKQEKELWDKQQKEQKEKLRREEQERKAGKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQSKSEHEASDAKVPVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTPMVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHKEENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRRRSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRITDVAT

Target ORF information:

RefSeq Version	NM_019729.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019729.3

ORF Insert Sequence:

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT AAAAGACCTC 
AATAAGAAGA CCGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATTATAT ACACAGCGCG 
CAGAAGATCT TCAAGACAGC AGAAGAATGC AGACTAGATC GTGATGAGGA AAGGGCCTAT 
GTGCTTTATA TGAAATATGT GGCAGTTTAT AATCTTATCA AAAAGAGACC TGATTTCAAG 
CAACAGCAGG ATTATTATCT TTCAATACTT GGACCTGCAA ACATCAAAAA AGCTATTGAA 
GAAGCTGAAA GACTCTCCGA AAGCCTTAAA CTAAGATACG AAGAGGCTGA AGTTCGGAAA 
CAACTTGAAG AAAAGGACAG ACGGGAGGAA GAGCAGCTGC AGCAACAGAA AAGGCAGGAG 
ATGGGGAGAG AGGACAGCGG TGCGGCAGCC AAACGCTCTG TGGAAAATTT ACTGGATTCC 
AAAACCAAAA CCCAAAGGAT TAACGGCGAG AAGAGTGAAG GAGCTGCAGC TGCAGAGAGA 
GGAGCCATCA CAGCAAAGGA ACTATATACG ATGATGATGG ATAAAAACAC AAGCTTAATT 
ATAATGGATG CTCGAAAAAT ACAGGATTAT CAGCATTCCT GTATCTTGGA TTCTCTCAGT 
GTTCCTGAAG AAGCTATCAG TCCAGGAGTC ACTGCTAGTT GGATTGAAGC AAACCTCTCA 
GATGATTCTA AAGACACATG GAAAAAGAGG GGGAGTGTGG ACTATGTGGT CCTTCTCGAC 
TGGTTTAGTT CAGCGAAAGA TTTGCTGCTT GGGACCACTC TACGGAGTCT GAAAGATGCA 
CTTTTCAAGT GGGAAAGTAA AACTGTCCTG CGCCATGAGC CTTTGGTGTT GGAGGGCGGC 
TATGAAAACT GGCTGCTTTG CTACCCGCAG TTTACAACCA ATGCTAAGGT CACTCCACCC 
CCTCGGAGCA GAGCTGAAGA GGTGTCTGTC TCATTGGATT TTACTTATCC CTCATTGGAA 
GAACCAGTTC CTTCCAAACT TCCTACCCAG ATGCCACCTC CTCCTATAGA AACAAATGAA 
AAGGCACTGT TGGTAACTGA TCAAGATGAA AAGCTGAGAC TGTCAACCCA GCCAGCTCTA 
GCTGGACCTG GTGCGGCTCC CAGAGCTGAA GCCTCACCCA TAATTCAGCC AGCGCCTGCT 
ACAAAGAGTG TTCCACAGGT TGATCGTACG AAAAAACCGT CAGTCAAGTT GCCTGAAGAT 
CATAGAATAA AATCTGAAAA TACAGATCAG AGTGGAAGAG TTCTTTCTGA TCGATCCACC 
AAGCCAGTAT TTCCCTCTCC AACCACCATG TTAACAGATG AAGAAAAGGC TCGTATTCAT 
CAAGAAACTG CCCTTCTTAT GGAAAAGAAT AAACAGGAGA AGGAACTTTG GGACAAGCAG 
CAGAAGGAAC AGAAAGAGAA GCTGAGAAGG GAGGAACAAG AGCGCAAAGC TGGAAAGACA 
CAGGATGCAG ATGAACGTGA CTCCACTGAG AATCAGCACA AAGCAAAGGA TGGACAAGAG 
AAAAAAGACA GCAAACAGAC CAAGACGGAA GACAGAGAGC TTTCAGCAGA CGGGGCCCAG 
GAAGCCACAG GAACACAAAG ACAAAGTAAG AGTGAGCATG AAGCTTCTGA TGCTAAGGTA 
CCTGTGGAAG GTAAAAGGTG TCCCACGTCA GAGGCGCAGA AGAGGCCAGC AGATGTGTCC 
CCTGCATCCG TGTCAGGAGA GCTGAATGCA GGCAAGGCTC AGCGAGAACC TTTGACGAGA 
GCACGAAGTG AAGAAATGGG GAGAATTGTG CCGGGACTGC CTTTGGGCTG GGCCAAGTTT 
CTTGATCCAA TCACCGGGAC CTTTCGTTAC TACCATTCCC CCACAAACAC AGTTCATATG 
TATCCACCTG AAATGGCTCC TTCGTCTGCA CCTCCTTCCA CCCCGCCCAC TCACAAAGTC 
AAGCCCCAGG TCCCTGCCGA GCGGGACAGG GAGCCATCGA AACTGAAGCG CTCCTACTCC 
TCACCAGATA TCACTCAGGC CCTGCAGGAG GAGGAGAAGA GGAGGCCGGC AGTGACCCCG 
ATGGTCAACC GGGAAAACAA GCCACCATGT TACCCTAAAG CTGAGATTTC GAGGCTTTCT 
GCTTCTCAGA TTCGGAACCT CAATCCTGTA TTTGGAGGAT CAGGACCAGC TCTTACTGGA 
CTTCGTAATT TGGGAAATAC TTGTTACATG AACTCAATCT TGCAGTGCCT GTGTAATGCT 
CCACATCTGG CTGATTATTT CAACCGAAAC TGCTACCAGG ATGATATCAA CAGGTCAAAT 
TTGTTGGGGC ATAAAGGTGA AGTGGCAGAA GAATTTGGTA TAATCATGAA AGCACTGTGG 
ACAGGACAGT ATAGATACAT CAGTCCAAAG GACTTTAAAG TCACCATTGG TAAGATTAAT 
GACCAGTTTG CAGGCTCCAG CCAACAGGAT TCACAAGAGC TGCTTCTGTT CCTCATGGAT 
GGCCTGCATG AGGATCTGAA TAAGGCTGAC AATCGGAAGA GGCACAAGGA AGAGAACAAC 
GAGCACCTGG ATGACCTGCA GGCGGCCGAG CACGCCTGGC AGAAGCACAA GCAGCTCAAC 
GAGTCCATCA TCGTGGCCCT GTTCCAGGGC CAGTTCAAGT CCACAGTGCA GTGCCTCACC 
TGCCGCAGGA GGTCGCGCAC CTTCGAGGCC TTCATGTACT TGTCTTTGCC GCTAGCATCC 
ACAAGTAAAT GTACTCTACA GGACTGCCTT AGATTATTTT CCAAAGAAGA AAAGCTTACA 
GATAACAACA GATTTTACTG CAGCCATTGC CGAGCTCGGC GGGATTCTCT AAAGAAAATA 
GAAATCTGGA AATTACCTCC TGTGCTGTTA GTGCACCTGA AACGATTTTC CTATGACGGC 
AGGTGGAAGC AGAAGCTGCA AACATCCGTG GATTTCCCAT TGGAAAATCT TGACCTGTCA 
CAGTACGTTA TTGGTCCAAA AAACAGCTTG AAGAAATATA ACTTGTTTTC TGTTTCAAAC 
CACTACGGCG GGCTAGACGG AGGCCACTAC ACAGCCTACT GTAAGAACGC GGCAAGGCAG 
CGCTGGTTTA AGTTTGATGA CCATGAAGTT TCTGATATCT CTGTGTCTTC TGTGAGGTCA 
TCAGCAGCTT ATATCCTCTT TTATACTTCC CTGGGACCAC GCATAACTGA TGTAGCCACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp8 ( XM_011239857.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011238159.1 Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.

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CloneID	OMu71879
Clone ID Related Accession (Same CDS sequence)	XM_011239857.2 , XM_011239857.3
Accession Version	XM_011239857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011239857.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATATG TGGCAGTTTA TAATCTTATC AAAAAGAGAC CTGATTTCAA GCAACAGCAG 
GATTATTATC TTTCAATACT TGGACCTGCA AACATCAAAA AAGCTATTGA AGAAGCTGAA 
AGACTCTCCG AAAGCCTTAA ACTAAGATAC GAAGAGGCTG AAGTTCGGAA ACAACTTGAA 
GAAAAGGACA GACGGGAGGA AGAGCAGCTG CAGCAACAGA AAAGGCAGGA GATGGGGAGA 
GAGGACAGCG GTGCGGCAGC CAAACGCTCT GTGGAAAATT TACTGGATTC CAAAACCAAA 
ACCCAAAGGA TTAACGGCGA GAAGAGTGAA GGAGCTGCAG CTGCAGAGAG AGGAGCCATC 
ACAGCAAAGG AACTATATAC GATGATGATG GATAAAAACA CAAGCTTAAT TATAATGGAT 
GCTCGAAAAA TACAGGATTA TCAGCATTCC TGTATCTTGG ATTCTCTCAG TGTTCCTGAA 
GAAGCTATCA GTCCAGGAGT CACTGCTAGT TGGATTGAAG CAAACCTCTC AGATGATTCT 
AAAGACACAT GGAAAAAGAG GGGGAGTGTG GACTATGTGG TCCTTCTCGA CTGGTTTAGT 
TCAGCGAAAG ATTTGCTGCT TGGGACCACT CTACGGAGTC TGAAAGATGC ACTTTTCAAG 
TGGGAAAGTA AAACTGTCCT GCGCCATGAG CCTTTGGTGT TGGAGGGCGG CTATGAAAAC 
TGGCTGCTTT GCTACCCGCA GTTTACAACC AATGCTAAGG TCACTCCACC CCCTCGGAGC 
AGAGCTGAAG AGGTGTCTGT CTCATTGGAT TTTACTTATC CCTCATTGGA AGAACCAGTT 
CCTTCCAAAC TTCCTACCCA GATGCCACCT CCTCCTATAG AAACAAATGA AAAGGCACTG 
TTGGTAACTG ATCAAGATGA AAAGCTGAGA CTGTCAACCC AGCCAGCTCT AGCTGGACCT 
GGTGCGGCTC CCAGAGCTGA AGCCTCACCC ATAATTCAGC CAGCGCCTGC TACAAAGAGT 
GTTCCACAGG TTGATCGTAC GAAAAAACCG TCAGTCAAGT TGCCTGAAGA TCATAGAATA 
AAATCTGAAA ATACAGATCA GAGTGGAAGA GTTCTTTCTG ATCGATCCAC CAAGCCAGTA 
TTTCCCTCTC CAACCACCAT GTTAACAGAT GAAGAAAAGG CTCGTATTCA TCAAGAAACT 
GCCCTTCTTA TGGAAAAGAA TAAACAGGAG AAGGAACTTT GGGACAAGCA GCAGAAGGAA 
CAGAAAGAGA AGCTGAGAAG GGAGGAACAA GAGCGCAAAG CTGGAAAGAC ACAGGATGCA 
GATGAACGTG ACTCCACTGA GAATCAGCAC AAAGCAAAGG ATGGACAAGA GAAAAAAGAC 
AGCAAACAGA CCAAGACGGA AGACAGAGAG CTTTCAGCAG ACGGGGCCCA GGAAGCCACA 
GGAACACAAA GACAAAGTAA GAGTGAGCAT GAAGCTTCTG ATGCTAAGGT ACCTGTGGAA 
GGTAAAAGGT GTCCCACGTC AGAGGCGCAG AAGAGGCCAG CAGATGTGTC CCCTGCATCC 
GTGTCAGGAG AGCTGAATGC AGGCAAGGCT CAGCGAGAAC CTTTGACGAG AGCACGAAGT 
GAAGAAATGG GGAGAATTGT GCCGGGACTG CCTTTGGGCT GGGCCAAGTT TCTTGATCCA 
ATCACCGGGA CCTTTCGTTA CTACCATTCC CCCACAAACA CAGTTCATAT GTATCCACCT 
GAAATGGCTC CTTCGTCTGC ACCTCCTTCC ACCCCGCCCA CTCACAAAGT CAAGCCCCAG 
GTCCCTGCCG AGCGGGACAG GGAGCCATCG AAACTGAAGC GCTCCTACTC CTCACCAGAT 
ATCACTCAGG CCCTGCAGGA GGAGGAGAAG AGGAGGCCGG CAGTGACCCC GATGGTCAAC 
CGGGAAAACA AGCCACCATG TTACCCTAAA GCTGAGATTT CGAGGCTTTC TGCTTCTCAG 
ATTCGGAACC TCAATCCTGT ATTTGGAGGA TCAGGACCAG CTCTTACTGG ACTTCGTAAT 
TTGGGAAATA CTTGTTACAT GAACTCAATC TTGCAGTGCC TGTGTAATGC TCCACATCTG 
GCTGATTATT TCAACCGAAA CTGCTACCAG GATGATATCA ACAGGTCAAA TTTGTTGGGG 
CATAAAGGTG AAGTGGCAGA AGAATTTGGT ATAATCATGA AAGCACTGTG GACAGGACAG 
TATAGATACA TCAGTCCAAA GGACTTTAAA GTCACCATTG GTAAGATTAA TGACCAGTTT 
GCAGGCTCCA GCCAACAGGA TTCACAAGAG CTGCTTCTGT TCCTCATGGA TGGCCTGCAT 
GAGGATCTGA ATAAGGCTGA CAATCGGAAG AGGCACAAGG AAGAGAACAA CGAGCACCTG 
GATGACCTGC AGGCGGCCGA GCACGCCTGG CAGAAGCACA AGCAGCTCAA CGAGTCCATC 
ATCGTGGCCC TGTTCCAGGG CCAGTTCAAG TCCACAGTGC AGTGCCTCAC CTGCCGCAGG 
AGGTCGCGCA CCTTCGAGGC CTTCATGTAC TTGTCTTTGC CGCTAGCATC CACAAGTAAA 
TGTACTCTAC AGGACTGCCT TAGATTATTT TCCAAAGAAG AAAAGCTTAC AGATAACAAC 
AGATTTTACT GCAGCCATTG CCGAGCTCGG CGGGATTCTC TAAAGAAAAT AGAAATCTGG 
AAATTACCTC CTGTGCTGTT AGTGCACCTG AAACGATTTT CCTATGACGG CAGGTGGAAG 
CAGAAGCTGC AAACATCCGT GGATTTCCCA TTGGAAAATC TTGACCTGTC ACAGTACGTT 
ATTGGTCCAA AAAACAGCTT GAAGAAATAT AACTTGTTTT CTGTTTCAAA CCACTACGGC 
GGGCTAGACG GAGGCCACTA CACAGCCTAC TGTAAGAACG CGGCAAGGCA GCGCTGGTTT 
AAGTTTGATG ACCATGAAGT TTCTGATATC TCTGTGTCTT CTGTGAGGTC ATCAGCAGCT 
TATATCCTCT TTTATACTTC CCTGGGACCA CGCATAACTG ATGTAGCCAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011238159.1
CDS	566..3619
Translation	MKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKTQRINGEKSEGAAAAERGAITAKELYTMMMDKNTSLIIMDARKIQDYQHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDALFKWESKTVLRHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRAEEVSVSLDFTYPSLEEPVPSKLPTQMPPPPIETNEKALLVTDQDEKLRLSTQPALAGPGAAPRAEASPIIQPAPATKSVPQVDRTKKPSVKLPEDHRIKSENTDQSGRVLSDRSTKPVFPSPTTMLTDEEKARIHQETALLMEKNKQEKELWDKQQKEQKEKLRREEQERKAGKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQSKSEHEASDAKVPVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTPMVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHKEENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRRRSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRITDVAT

Target ORF information:

RefSeq Version	XM_011239857.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239857.3

ORF Insert Sequence:

ATGAAATATG TGGCAGTTTA TAATCTTATC AAAAAGAGAC CTGATTTCAA GCAACAGCAG 
GATTATTATC TTTCAATACT TGGACCTGCA AACATCAAAA AAGCTATTGA AGAAGCTGAA 
AGACTCTCCG AAAGCCTTAA ACTAAGATAC GAAGAGGCTG AAGTTCGGAA ACAACTTGAA 
GAAAAGGACA GACGGGAGGA AGAGCAGCTG CAGCAACAGA AAAGGCAGGA GATGGGGAGA 
GAGGACAGCG GTGCGGCAGC CAAACGCTCT GTGGAAAATT TACTGGATTC CAAAACCAAA 
ACCCAAAGGA TTAACGGCGA GAAGAGTGAA GGAGCTGCAG CTGCAGAGAG AGGAGCCATC 
ACAGCAAAGG AACTATATAC GATGATGATG GATAAAAACA CAAGCTTAAT TATAATGGAT 
GCTCGAAAAA TACAGGATTA TCAGCATTCC TGTATCTTGG ATTCTCTCAG TGTTCCTGAA 
GAAGCTATCA GTCCAGGAGT CACTGCTAGT TGGATTGAAG CAAACCTCTC AGATGATTCT 
AAAGACACAT GGAAAAAGAG GGGGAGTGTG GACTATGTGG TCCTTCTCGA CTGGTTTAGT 
TCAGCGAAAG ATTTGCTGCT TGGGACCACT CTACGGAGTC TGAAAGATGC ACTTTTCAAG 
TGGGAAAGTA AAACTGTCCT GCGCCATGAG CCTTTGGTGT TGGAGGGCGG CTATGAAAAC 
TGGCTGCTTT GCTACCCGCA GTTTACAACC AATGCTAAGG TCACTCCACC CCCTCGGAGC 
AGAGCTGAAG AGGTGTCTGT CTCATTGGAT TTTACTTATC CCTCATTGGA AGAACCAGTT 
CCTTCCAAAC TTCCTACCCA GATGCCACCT CCTCCTATAG AAACAAATGA AAAGGCACTG 
TTGGTAACTG ATCAAGATGA AAAGCTGAGA CTGTCAACCC AGCCAGCTCT AGCTGGACCT 
GGTGCGGCTC CCAGAGCTGA AGCCTCACCC ATAATTCAGC CAGCGCCTGC TACAAAGAGT 
GTTCCACAGG TTGATCGTAC GAAAAAACCG TCAGTCAAGT TGCCTGAAGA TCATAGAATA 
AAATCTGAAA ATACAGATCA GAGTGGAAGA GTTCTTTCTG ATCGATCCAC CAAGCCAGTA 
TTTCCCTCTC CAACCACCAT GTTAACAGAT GAAGAAAAGG CTCGTATTCA TCAAGAAACT 
GCCCTTCTTA TGGAAAAGAA TAAACAGGAG AAGGAACTTT GGGACAAGCA GCAGAAGGAA 
CAGAAAGAGA AGCTGAGAAG GGAGGAACAA GAGCGCAAAG CTGGAAAGAC ACAGGATGCA 
GATGAACGTG ACTCCACTGA GAATCAGCAC AAAGCAAAGG ATGGACAAGA GAAAAAAGAC 
AGCAAACAGA CCAAGACGGA AGACAGAGAG CTTTCAGCAG ACGGGGCCCA GGAAGCCACA 
GGAACACAAA GACAAAGTAA GAGTGAGCAT GAAGCTTCTG ATGCTAAGGT ACCTGTGGAA 
GGTAAAAGGT GTCCCACGTC AGAGGCGCAG AAGAGGCCAG CAGATGTGTC CCCTGCATCC 
GTGTCAGGAG AGCTGAATGC AGGCAAGGCT CAGCGAGAAC CTTTGACGAG AGCACGAAGT 
GAAGAAATGG GGAGAATTGT GCCGGGACTG CCTTTGGGCT GGGCCAAGTT TCTTGATCCA 
ATCACCGGGA CCTTTCGTTA CTACCATTCC CCCACAAACA CAGTTCATAT GTATCCACCT 
GAAATGGCTC CTTCGTCTGC ACCTCCTTCC ACCCCGCCCA CTCACAAAGT CAAGCCCCAG 
GTCCCTGCCG AGCGGGACAG GGAGCCATCG AAACTGAAGC GCTCCTACTC CTCACCAGAT 
ATCACTCAGG CCCTGCAGGA GGAGGAGAAG AGGAGGCCGG CAGTGACCCC GATGGTCAAC 
CGGGAAAACA AGCCACCATG TTACCCTAAA GCTGAGATTT CGAGGCTTTC TGCTTCTCAG 
ATTCGGAACC TCAATCCTGT ATTTGGAGGA TCAGGACCAG CTCTTACTGG ACTTCGTAAT 
TTGGGAAATA CTTGTTACAT GAACTCAATC TTGCAGTGCC TGTGTAATGC TCCACATCTG 
GCTGATTATT TCAACCGAAA CTGCTACCAG GATGATATCA ACAGGTCAAA TTTGTTGGGG 
CATAAAGGTG AAGTGGCAGA AGAATTTGGT ATAATCATGA AAGCACTGTG GACAGGACAG 
TATAGATACA TCAGTCCAAA GGACTTTAAA GTCACCATTG GTAAGATTAA TGACCAGTTT 
GCAGGCTCCA GCCAACAGGA TTCACAAGAG CTGCTTCTGT TCCTCATGGA TGGCCTGCAT 
GAGGATCTGA ATAAGGCTGA CAATCGGAAG AGGCACAAGG AAGAGAACAA CGAGCACCTG 
GATGACCTGC AGGCGGCCGA GCACGCCTGG CAGAAGCACA AGCAGCTCAA CGAGTCCATC 
ATCGTGGCCC TGTTCCAGGG CCAGTTCAAG TCCACAGTGC AGTGCCTCAC CTGCCGCAGG 
AGGTCGCGCA CCTTCGAGGC CTTCATGTAC TTGTCTTTGC CGCTAGCATC CACAAGTAAA 
TGTACTCTAC AGGACTGCCT TAGATTATTT TCCAAAGAAG AAAAGCTTAC AGATAACAAC 
AGATTTTACT GCAGCCATTG CCGAGCTCGG CGGGATTCTC TAAAGAAAAT AGAAATCTGG 
AAATTACCTC CTGTGCTGTT AGTGCACCTG AAACGATTTT CCTATGACGG CAGGTGGAAG 
CAGAAGCTGC AAACATCCGT GGATTTCCCA TTGGAAAATC TTGACCTGTC ACAGTACGTT 
ATTGGTCCAA AAAACAGCTT GAAGAAATAT AACTTGTTTT CTGTTTCAAA CCACTACGGC 
GGGCTAGACG GAGGCCACTA CACAGCCTAC TGTAAGAACG CGGCAAGGCA GCGCTGGTTT 
AAGTTTGATG ACCATGAAGT TTCTGATATC TCTGTGTCTT CTGTGAGGTC ATCAGCAGCT 
TATATCCTCT TTTATACTTC CCTGGGACCA CGCATAACTG ATGTAGCCAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp8 ( NM_001252580.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001239509.1 Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant 1, mRNA.

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CloneID	OMu05291
Clone ID Related Accession (Same CDS sequence)	NM_001252580.1
Accession Version	NM_001252580.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	ubiquitin carboxyl-terminal hydrolase 8 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY147904.1, AK129003.1 and BY639666.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129003.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164133, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT AAAAGACCTC 
AATAAGAAGA CCGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATACCAA GTTTTGTAAA 
TTCTCTTCGT TTCTAAGTTA TATACACAGC GCGCAGAAGA TCTTCAAGAC AGCAGAAGAA 
TGCAGACTAG ATCGTGATGA GGAAAGGGCC TATGTGCTTT ATATGAAATA TGTGGCAGTT 
TATAATCTTA TCAAAAAGAG ACCTGATTTC AAGCAACAGC AGGATTATTA TCTTTCAATA 
CTTGGACCTG CAAACATCAA AAAAGCTATT GAAGAAGCTG AAAGACTCTC CGAAAGCCTT 
AAACTAAGAT ACGAAGAGGC TGAAGTTCGG AAACAACTTG AAGAAAAGGA CAGACGGGAG 
GAAGAGCAGC TGCAGCAACA GAAAAGGCAG GAGATGGGGA GAGAGGACAG CGGTGCGGCA 
GCCAAACGCT CTGTGGAAAA TTTACTGGAT TCCAAAACCA AAACCCAAAG GATTAACGGC 
GAGAAGAGTG AAGGAGCTGC AGCTGCAGAG AGAGGAGCCA TCACAGCAAA GGAACTATAT 
ACGATGATGA TGGATAAAAA CACAAGCTTA ATTATAATGG ATGCTCGAAA AATACAGGAT 
TATCAGCATT CCTGTATCTT GGATTCTCTC AGTGTTCCTG AAGAAGCTAT CAGTCCAGGA 
GTCACTGCTA GTTGGATTGA AGCAAACCTC TCAGATGATT CTAAAGACAC ATGGAAAAAG 
AGGGGGAGTG TGGACTATGT GGTCCTTCTC GACTGGTTTA GTTCAGCGAA AGATTTGCTG 
CTTGGGACCA CTCTACGGAG TCTGAAAGAT GCACTTTTCA AGTGGGAAAG TAAAACTGTC 
CTGCGCCATG AGCCTTTGGT GTTGGAGGGC GGCTATGAAA ACTGGCTGCT TTGCTACCCG 
CAGTTTACAA CCAATGCTAA GGTCACTCCA CCCCCTCGGA GCAGAGCTGA AGAGGTGTCT 
GTCTCATTGG ATTTTACTTA TCCCTCATTG GAAGAACCAG TTCCTTCCAA ACTTCCTACC 
CAGATGCCAC CTCCTCCTAT AGAAACAAAT GAAAAGGCAC TGTTGGTAAC TGATCAAGAT 
GAAAAGCTGA GACTGTCAAC CCAGCCAGCT CTAGCTGGAC CTGGTGCGGC TCCCAGAGCT 
GAAGCCTCAC CCATAATTCA GCCAGCGCCT GCTACAAAGA GTGTTCCACA GGTTGATCGT 
ACGAAAAAAC CGTCAGTCAA GTTGCCTGAA GATCATAGAA TAAAATCTGA AAATACAGAT 
CAGAGTGGAA GAGTTCTTTC TGATCGATCC ACCAAGCCAG TATTTCCCTC TCCAACCACC 
ATGTTAACAG ATGAAGAAAA GGCTCGTATT CATCAAGAAA CTGCCCTTCT TATGGAAAAG 
AATAAACAGG AGAAGGAACT TTGGGACAAG CAGCAGAAGG AACAGAAAGA GAAGCTGAGA 
AGGGAGGAAC AAGAGCGCAA AGCTGGAAAG ACACAGGATG CAGATGAACG TGACTCCACT 
GAGAATCAGC ACAAAGCAAA GGATGGACAA GAGAAAAAAG ACAGCAAACA GACCAAGACG 
GAAGACAGAG AGCTTTCAGC AGACGGGGCC CAGGAAGCCA CAGGAACACA AAGACAAAGT 
AAGAGTGAGC ATGAAGCTTC TGATGCTAAG GTACCTGTGG AAGGTAAAAG GTGTCCCACG 
TCAGAGGCGC AGAAGAGGCC AGCAGATGTG TCCCCTGCAT CCGTGTCAGG AGAGCTGAAT 
GCAGGCAAGG CTCAGCGAGA ACCTTTGACG AGAGCACGAA GTGAAGAAAT GGGGAGAATT 
GTGCCGGGAC TGCCTTTGGG CTGGGCCAAG TTTCTTGATC CAATCACCGG GACCTTTCGT 
TACTACCATT CCCCCACAAA CACAGTTCAT ATGTATCCAC CTGAAATGGC TCCTTCGTCT 
GCACCTCCTT CCACCCCGCC CACTCACAAA GTCAAGCCCC AGGTCCCTGC CGAGCGGGAC 
AGGGAGCCAT CGAAACTGAA GCGCTCCTAC TCCTCACCAG ATATCACTCA GGCCCTGCAG 
GAGGAGGAGA AGAGGAGGCC GGCAGTGACC CCGATGGTCA ACCGGGAAAA CAAGCCACCA 
TGTTACCCTA AAGCTGAGAT TTCGAGGCTT TCTGCTTCTC AGATTCGGAA CCTCAATCCT 
GTATTTGGAG GATCAGGACC AGCTCTTACT GGACTTCGTA ATTTGGGAAA TACTTGTTAC 
ATGAACTCAA TCTTGCAGTG CCTGTGTAAT GCTCCACATC TGGCTGATTA TTTCAACCGA 
AACTGCTACC AGGATGATAT CAACAGGTCA AATTTGTTGG GGCATAAAGG TGAAGTGGCA 
GAAGAATTTG GTATAATCAT GAAAGCACTG TGGACAGGAC AGTATAGATA CATCAGTCCA 
AAGGACTTTA AAGTCACCAT TGGTAAGATT AATGACCAGT TTGCAGGCTC CAGCCAACAG 
GATTCACAAG AGCTGCTTCT GTTCCTCATG GATGGCCTGC ATGAGGATCT GAATAAGGCT 
GACAATCGGA AGAGGCACAA GGAAGAGAAC AACGAGCACC TGGATGACCT GCAGGCGGCC 
GAGCACGCCT GGCAGAAGCA CAAGCAGCTC AACGAGTCCA TCATCGTGGC CCTGTTCCAG 
GGCCAGTTCA AGTCCACAGT GCAGTGCCTC ACCTGCCGCA GGAGGTCGCG CACCTTCGAG 
GCCTTCATGT ACTTGTCTTT GCCGCTAGCA TCCACAAGTA AATGTACTCT ACAGGACTGC 
CTTAGATTAT TTTCCAAAGA AGAAAAGCTT ACAGATAACA ACAGATTTTA CTGCAGCCAT 
TGCCGAGCTC GGCGGGATTC TCTAAAGAAA ATAGAAATCT GGAAATTACC TCCTGTGCTG 
TTAGTGCACC TGAAACGATT TTCCTATGAC GGCAGGTGGA AGCAGAAGCT GCAAACATCC 
GTGGATTTCC CATTGGAAAA TCTTGACCTG TCACAGTACG TTATTGGTCC AAAAAACAGC 
TTGAAGAAAT ATAACTTGTT TTCTGTTTCA AACCACTACG GCGGGCTAGA CGGAGGCCAC 
TACACAGCCT ACTGTAAGAA CGCGGCAAGG CAGCGCTGGT TTAAGTTTGA TGACCATGAA 
GTTTCTGATA TCTCTGTGTC TTCTGTGAGG TCATCAGCAG CTTATATCCT CTTTTATACT 
TCCCTGGGAC CACGCATAAC TGATGTAGCC ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001239509.1
CDS	227..3502
Translation	MPAVASVPKELYLSSSLKDLNKKTEVKPEKTSTKNTKFCKFSSFLSYIHSAQKIFKTAEECRLDRDEERAYVLYMKYVAVYNLIKKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLRYEEAEVRKQLEEKDRREEEQLQQQKRQEMGREDSGAAAKRSVENLLDSKTKTQRINGEKSEGAAAAERGAITAKELYTMMMDKNTSLIIMDARKIQDYQHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLGTTLRSLKDALFKWESKTVLRHEPLVLEGGYENWLLCYPQFTTNAKVTPPPRSRAEEVSVSLDFTYPSLEEPVPSKLPTQMPPPPIETNEKALLVTDQDEKLRLSTQPALAGPGAAPRAEASPIIQPAPATKSVPQVDRTKKPSVKLPEDHRIKSENTDQSGRVLSDRSTKPVFPSPTTMLTDEEKARIHQETALLMEKNKQEKELWDKQQKEQKEKLRREEQERKAGKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQSKSEHEASDAKVPVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGLPLGWAKFLDPITGTFRYYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLKRSYSSPDITQALQEEEKRRPAVTPMVNRENKPPCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRKRHKEENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRRRSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNSLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVRSSAAYILFYTSLGPRITDVAT

Target ORF information:

RefSeq Version	NM_001252580.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin specific peptidase 8 (Usp8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001252580.1

ORF Insert Sequence:

ATGCCTGCTG TAGCTTCAGT TCCTAAAGAA CTCTACCTCA GTTCTTCACT AAAAGACCTC 
AATAAGAAGA CCGAAGTTAA ACCTGAGAAA ACCAGCACCA AGAATACCAA GTTTTGTAAA 
TTCTCTTCGT TTCTAAGTTA TATACACAGC GCGCAGAAGA TCTTCAAGAC AGCAGAAGAA 
TGCAGACTAG ATCGTGATGA GGAAAGGGCC TATGTGCTTT ATATGAAATA TGTGGCAGTT 
TATAATCTTA TCAAAAAGAG ACCTGATTTC AAGCAACAGC AGGATTATTA TCTTTCAATA 
CTTGGACCTG CAAACATCAA AAAAGCTATT GAAGAAGCTG AAAGACTCTC CGAAAGCCTT 
AAACTAAGAT ACGAAGAGGC TGAAGTTCGG AAACAACTTG AAGAAAAGGA CAGACGGGAG 
GAAGAGCAGC TGCAGCAACA GAAAAGGCAG GAGATGGGGA GAGAGGACAG CGGTGCGGCA 
GCCAAACGCT CTGTGGAAAA TTTACTGGAT TCCAAAACCA AAACCCAAAG GATTAACGGC 
GAGAAGAGTG AAGGAGCTGC AGCTGCAGAG AGAGGAGCCA TCACAGCAAA GGAACTATAT 
ACGATGATGA TGGATAAAAA CACAAGCTTA ATTATAATGG ATGCTCGAAA AATACAGGAT 
TATCAGCATT CCTGTATCTT GGATTCTCTC AGTGTTCCTG AAGAAGCTAT CAGTCCAGGA 
GTCACTGCTA GTTGGATTGA AGCAAACCTC TCAGATGATT CTAAAGACAC ATGGAAAAAG 
AGGGGGAGTG TGGACTATGT GGTCCTTCTC GACTGGTTTA GTTCAGCGAA AGATTTGCTG 
CTTGGGACCA CTCTACGGAG TCTGAAAGAT GCACTTTTCA AGTGGGAAAG TAAAACTGTC 
CTGCGCCATG AGCCTTTGGT GTTGGAGGGC GGCTATGAAA ACTGGCTGCT TTGCTACCCG 
CAGTTTACAA CCAATGCTAA GGTCACTCCA CCCCCTCGGA GCAGAGCTGA AGAGGTGTCT 
GTCTCATTGG ATTTTACTTA TCCCTCATTG GAAGAACCAG TTCCTTCCAA ACTTCCTACC 
CAGATGCCAC CTCCTCCTAT AGAAACAAAT GAAAAGGCAC TGTTGGTAAC TGATCAAGAT 
GAAAAGCTGA GACTGTCAAC CCAGCCAGCT CTAGCTGGAC CTGGTGCGGC TCCCAGAGCT 
GAAGCCTCAC CCATAATTCA GCCAGCGCCT GCTACAAAGA GTGTTCCACA GGTTGATCGT 
ACGAAAAAAC CGTCAGTCAA GTTGCCTGAA GATCATAGAA TAAAATCTGA AAATACAGAT 
CAGAGTGGAA GAGTTCTTTC TGATCGATCC ACCAAGCCAG TATTTCCCTC TCCAACCACC 
ATGTTAACAG ATGAAGAAAA GGCTCGTATT CATCAAGAAA CTGCCCTTCT TATGGAAAAG 
AATAAACAGG AGAAGGAACT TTGGGACAAG CAGCAGAAGG AACAGAAAGA GAAGCTGAGA 
AGGGAGGAAC AAGAGCGCAA AGCTGGAAAG ACACAGGATG CAGATGAACG TGACTCCACT 
GAGAATCAGC ACAAAGCAAA GGATGGACAA GAGAAAAAAG ACAGCAAACA GACCAAGACG 
GAAGACAGAG AGCTTTCAGC AGACGGGGCC CAGGAAGCCA CAGGAACACA AAGACAAAGT 
AAGAGTGAGC ATGAAGCTTC TGATGCTAAG GTACCTGTGG AAGGTAAAAG GTGTCCCACG 
TCAGAGGCGC AGAAGAGGCC AGCAGATGTG TCCCCTGCAT CCGTGTCAGG AGAGCTGAAT 
GCAGGCAAGG CTCAGCGAGA ACCTTTGACG AGAGCACGAA GTGAAGAAAT GGGGAGAATT 
GTGCCGGGAC TGCCTTTGGG CTGGGCCAAG TTTCTTGATC CAATCACCGG GACCTTTCGT 
TACTACCATT CCCCCACAAA CACAGTTCAT ATGTATCCAC CTGAAATGGC TCCTTCGTCT 
GCACCTCCTT CCACCCCGCC CACTCACAAA GTCAAGCCCC AGGTCCCTGC CGAGCGGGAC 
AGGGAGCCAT CGAAACTGAA GCGCTCCTAC TCCTCACCAG ATATCACTCA GGCCCTGCAG 
GAGGAGGAGA AGAGGAGGCC GGCAGTGACC CCGATGGTCA ACCGGGAAAA CAAGCCACCA 
TGTTACCCTA AAGCTGAGAT TTCGAGGCTT TCTGCTTCTC AGATTCGGAA CCTCAATCCT 
GTATTTGGAG GATCAGGACC AGCTCTTACT GGACTTCGTA ATTTGGGAAA TACTTGTTAC 
ATGAACTCAA TCTTGCAGTG CCTGTGTAAT GCTCCACATC TGGCTGATTA TTTCAACCGA 
AACTGCTACC AGGATGATAT CAACAGGTCA AATTTGTTGG GGCATAAAGG TGAAGTGGCA 
GAAGAATTTG GTATAATCAT GAAAGCACTG TGGACAGGAC AGTATAGATA CATCAGTCCA 
AAGGACTTTA AAGTCACCAT TGGTAAGATT AATGACCAGT TTGCAGGCTC CAGCCAACAG 
GATTCACAAG AGCTGCTTCT GTTCCTCATG GATGGCCTGC ATGAGGATCT GAATAAGGCT 
GACAATCGGA AGAGGCACAA GGAAGAGAAC AACGAGCACC TGGATGACCT GCAGGCGGCC 
GAGCACGCCT GGCAGAAGCA CAAGCAGCTC AACGAGTCCA TCATCGTGGC CCTGTTCCAG 
GGCCAGTTCA AGTCCACAGT GCAGTGCCTC ACCTGCCGCA GGAGGTCGCG CACCTTCGAG 
GCCTTCATGT ACTTGTCTTT GCCGCTAGCA TCCACAAGTA AATGTACTCT ACAGGACTGC 
CTTAGATTAT TTTCCAAAGA AGAAAAGCTT ACAGATAACA ACAGATTTTA CTGCAGCCAT 
TGCCGAGCTC GGCGGGATTC TCTAAAGAAA ATAGAAATCT GGAAATTACC TCCTGTGCTG 
TTAGTGCACC TGAAACGATT TTCCTATGAC GGCAGGTGGA AGCAGAAGCT GCAAACATCC 
GTGGATTTCC CATTGGAAAA TCTTGACCTG TCACAGTACG TTATTGGTCC AAAAAACAGC 
TTGAAGAAAT ATAACTTGTT TTCTGTTTCA AACCACTACG GCGGGCTAGA CGGAGGCCAC 
TACACAGCCT ACTGTAAGAA CGCGGCAAGG CAGCGCTGGT TTAAGTTTGA TGACCATGAA 
GTTTCTGATA TCTCTGTGTC TTCTGTGAGG TCATCAGCAG CTTATATCCT CTTTTATACT 
TCCCTGGGAC CACGCATAAC TGATGTAGCC ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dynamic of contribution of UBPy-sorted cargo to acrosome biogenesis: effects of its derailment in a mouse model of globozoospermia, the infertile Vps54 (L967Q) mutant.
Cell and tissue research(2017 Mar)
Gioria M,Pasini ME,Berruti G

The ubiquitin-specific protease USP8 is critical for the development and homeostasis of T cells.
Nature immunology16(9)950-60(2015 Sep)
Dufner A,Kisser A,Niendorf S,Basters A,Reissig S,Sch?nle A,Aichem A,Kurz T,Schlosser A,Yablonski D,Groettrup M,Buch T,Waisman A,Schamel WW,Prinz M,Knobeloch KP

USP8/UBPy-regulated sorting and the development of sperm acrosome: the recruitment of MET.
Reproduction (Cambridge, England)149(6)633-44(2015 Jun)
Berruti G,Paiardi C

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Usp8 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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