Chia1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chia1
Entrez Gene ID	81600
Full Name	chitinase, acidic 1
Synonyms	2200003E03Rik,AMCase,Chia,YNL
General protein information	Preferred Names chitinase, acidic 1 Names acidic mammalian chitinase acidic chitinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 3 Map Location: 3\|3 F2.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_023186.3	NP_075675.2	acidic mammalian chitinase precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:65788	NP_955897.2
Danio rerio (zebrafish)	chia.4	NP_956740.1
Magnaporthe oryzae (rice blast fungus)	MGG_01247	XP_003714154.1
Bos taurus (cattle)	CHIA	NP_777124.1
Gallus gallus (chicken)	CHIA	NP_989760.1
Danio rerio (zebrafish)	chia.3	NP_998378.1
Homo sapiens (human)	CHIA	NP_970615.2
Xenopus tropicalis (tropical clawed frog)	chia	NP_001186489.1
	CHIA	XP_001104487.1
Canis lupus familiaris (dog)	CHIA	XP_537030.3
Mus musculus (house mouse)	Chia1	NP_075675.2
Rattus norvegicus (Norway rat)	Chia	NP_997469.1
Danio rerio (zebrafish)	zgc:173927	NP_001103511.1
Neurospora crassa	NCU03209	XP_965309.2

Gene Ontology
Bibliography

Related articles in PubMed

Mouse acidic mammalian chitinase exhibits transglycosylation activity at somatic tissue pH.
Wakita S, Kobayashi S, Kimura M, Kashimura A, Honda S, Sakaguchi M, Sugahara Y, Kamaya M, Matoska V, Bauer PO, Oyama F
FEBS letters591(20)3310-3318(2017 Oct)

Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease.
Van Dyken SJ, Liang HE, Naikawadi RP, Woodruff PG, Wolters PJ, Erle DJ, Locksley RM
Cell169(3)497-509.e13(2017 Apr)

Loss and Gain of Human Acidic Mammalian Chitinase Activity by Nonsynonymous SNPs.
Okawa K, Ohno M, Kashimura A, Kimura M, Kobayashi Y, Sakaguchi M, Sugahara Y, Kamaya M, Kino Y, Bauer PO, Oyama F
Molecular biology and evolution33(12)3183-3193(2016 Dec)

Acidic chitinase primes the protective immune response to gastrointestinal nematodes.
Vannella KM, Ramalingam TR, Hart KM, de Queiroz Prado R, Sciurba J, Barron L, Borthwick LA, Smith AD, Mentink-Kane M, White S, Thompson RW, Cheever AW, Bock K, Moore I, Fitz LJ, Urban JF Jr, Wynn TA
Nature immunology17(5)538-44(2016 May)

Breast regression protein-39 is not required for experimental autoimmune encephalomyelitis induction.
Cant? E, Espejo C, Costa C, Montalban X, Comabella M
Clinical immunology (Orlando, Fla.)160(2)133-41(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results indicate that mouse AMCase catalyzes hydrolysis as well as transglycosylation and suggest that this enzyme can play a novel role under physiological conditions in peripheral tissues, such as the lungs.
Title: Mouse acidic mammalian chitinase exhibits transglycosylation activity at somatic tissue pH.

AMCase-deficient mice exhibit premature morbidity and mortality, concomitant with accumulation of environmentally derived chitin polymers in the airways and expression of pro-fibrotic cytokines. Over time, these mice develop spontaneous pulmonary fibrosis.
Title: Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease.

we showed that M at the position 61 is highly conserved in the many mammals other than orangutan and humans. In addition, introduction of M61R and M61I to WT mouse AMCase lead to a significant reduction of its chitinolytic activity. Taken together, our present and previous data by Seibold et al. (2009) indicate that G339T (R61M) in humans is associated with asthma protection in the fashion of gain of function
Title: Loss and Gain of Human Acidic Mammalian Chitinase Activity by Nonsynonymous SNPs.

Data show that acidic mammalian chitinase (AMCase) functions as a critical initiator of protective type 2 responses to intestinal nematodes, but is largely dispensable for allergic responses in the lung.
Title: Acidic chitinase primes the protective immune response to gastrointestinal nematodes.

AMCase expression was significantly decreased in experimental autoimmune encephalitis mice compared to PBS-controls.
Title: Breast regression protein-39 is not required for experimental autoimmune encephalomyelitis induction.

The following Chia1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chia1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00537	NM_023186.3 Latest version!	Mus musculus chitinase, acidic 1 (Chia1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chia1 ( NM_023186.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075675.2 Mus musculus chitinase, acidic 1 (Chia1), mRNA.

Price & Availability↑

CloneID	OMu00537
Clone ID Related Accession (Same CDS sequence)	NM_023186.3
Accession Version	NM_023186.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-18
Organism	Mus musculus(house mouse)
Product	acidic mammalian chitinase precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC034548.1. On or before May 4, 2006 this sequence version replaced XM_986603.1, XM_986563.1, NM_023186.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC034548.1, BC011134.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849380 [ECO:0000348] ##Evidence-Data-END##

ATGGCCAAGC TACTTCTCGT CACAGGTCTG GCTCTTCTGC TGAATGCTCA GCTGGGGTCT 
GCCTACAATC TGATATGCTA TTTCACCAAC TGGGCCCAGT ATCGGCCAGG TCTGGGGAGC 
TTCAAGCCTG ATGACATTAA CCCCTGCCTG TGTACTCACC TGATCTATGC CTTTGCTGGG 
ATGCAGAACA ATGAGATCAC CACCATAGAA TGGAATGATG TTACTCTCTA TAAAGCTTTC 
AATGACTTGA AAAACAGGAA CAGCAAACTG AAAACCCTCC TGGCAATTGG AGGCTGGAAC 
TTTGGAACTG CTCCTTTCAC TACCATGGTT TCCACTTCTC AGAACCGCCA GACCTTCATT 
ACCTCAGTCA TCAAATTTCT GCGTCAGTAT GGGTTTGATG GACTGGACCT GGACTGGGAA 
TACCCAGGCT CACGTGGGAG CCCTCCTCAG GACAAGCATC TCTTCACTGT CCTGGTGAAG 
GAAATGCGTG AAGCTTTTGA GCAGGAGGCT ATTGAGAGCA ACAGGCCCAG ACTGATGGTT 
ACTGCTGCTG TAGCTGGTGG GATTTCCAAC ATCCAGGCTG GCTATGAGAT CCCTGAACTT 
TCTAAGTACC TGGATTTCAT CCATGTCATG ACATATGACC TCCATGGCTC CTGGGAGGGC 
TACACTGGGG AGAATAGTCC TCTTTACAAA TACCCTACTG AGACTGGTAG CAATGCCTAC 
CTCAATGTGG ATTATGTCAT GAACTATTGG AAGAACAATG GAGCCCCAGC TGAGAAGCTC 
ATTGTTGGAT TCCCAGAGTA TGGACACACC TTCATCCTGA GAAACCCCTC TGATAATGGA 
ATTGGTGCCC CTACCTCTGG TGATGGCCCT GCTGGGCCCT ATACCAGACA GGCTGGGTTC 
TGGGCCTACT ATGAGATTTG CACCTTTCTG AGAAGTGGAG CCACTGAGGT CTGGGATGCC 
TCCCAAGAAG TGCCCTATGC CTATAAGGCC AACGAGTGGC TTGGCTATGA CAATATCAAG 
AGCTTCAGTG TTAAGGCTCA GTGGCTTAAG CAGAACAATT TTGGAGGTGC CATGATCTGG 
GCCATTGACC TTGATGACTT CACTGGCTCT TTCTGTGATC AGGGAAAATT TCCTCTGACT 
TCTACTTTGA ACAAAGCCCT TGGCATATCC ACTGAAGGTT GCACAGCTCC TGACGTGCCT 
TCCGAGCCAG TGACTACTCC TCCAGGAAGT GGGAGTGGGG GTGGAAGCTC CGGAGGAAGC 
TCTGGAGGCA GTGGATTCTG TGCCGACAAA GCAGATGGCC TCTACCCTGT GGCAGATGAC 
AGAAATGCTT TTTGGCAGTG CATCAATGGA ATCACATACC AGCAGCATTG TCAAGCAGGG 
CTTGTTTTTG ATACCAGCTG TAATTGCTGC AACTGGCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075675.2
CDS	4..1425
Translation	MAKLLLVTGLALLLNAQLGSAYNLICYFTNWAQYRPGLGSFKPDDINPCLCTHLIYAFAGMQNNEITTIEWNDVTLYKAFNDLKNRNSKLKTLLAIGGWNFGTAPFTTMVSTSQNRQTFITSVIKFLRQYGFDGLDLDWEYPGSRGSPPQDKHLFTVLVKEMREAFEQEAIESNRPRLMVTAAVAGGISNIQAGYEIPELSKYLDFIHVMTYDLHGSWEGYTGENSPLYKYPTETGSNAYLNVDYVMNYWKNNGAPAEKLIVGFPEYGHTFILRNPSDNGIGAPTSGDGPAGPYTRQAGFWAYYEICTFLRSGATEVWDASQEVPYAYKANEWLGYDNIKSFSVKAQWLKQNNFGGAMIWAIDLDDFTGSFCDQGKFPLTSTLNKALGISTEGCTAPDVPSEPVTTPPGSGSGGGSSGGSSGGSGFCADKADGLYPVADDRNAFWQCINGITYQQHCQAGLVFDTSCNCCNWP

Target ORF information:

RefSeq Version	NM_023186.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chitinase, acidic 1 (Chia1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023186.3

ORF Insert Sequence:

ATGGCCAAGC TACTTCTCGT CACAGGTCTG GCTCTTCTGC TGAATGCTCA GCTGGGGTCT 
GCCTACAATC TGATATGCTA TTTCACCAAC TGGGCCCAGT ATCGGCCAGG TCTGGGGAGC 
TTCAAGCCTG ATGACATTAA CCCCTGCCTG TGTACTCACC TGATCTATGC CTTTGCTGGG 
ATGCAGAACA ATGAGATCAC CACCATAGAA TGGAATGATG TTACTCTCTA TAAAGCTTTC 
AATGACTTGA AAAACAGGAA CAGCAAACTG AAAACCCTCC TGGCAATTGG AGGCTGGAAC 
TTTGGAACTG CTCCTTTCAC TACCATGGTT TCCACTTCTC AGAACCGCCA GACCTTCATT 
ACCTCAGTCA TCAAATTTCT GCGTCAGTAT GGGTTTGATG GACTGGACCT GGACTGGGAA 
TACCCAGGCT CACGTGGGAG CCCTCCTCAG GACAAGCATC TCTTCACTGT CCTGGTGAAG 
GAAATGCGTG AAGCTTTTGA GCAGGAGGCT ATTGAGAGCA ACAGGCCCAG ACTGATGGTT 
ACTGCTGCTG TAGCTGGTGG GATTTCCAAC ATCCAGGCTG GCTATGAGAT CCCTGAACTT 
TCTAAGTACC TGGATTTCAT CCATGTCATG ACATATGACC TCCATGGCTC CTGGGAGGGC 
TACACTGGGG AGAATAGTCC TCTTTACAAA TACCCTACTG AGACTGGTAG CAATGCCTAC 
CTCAATGTGG ATTATGTCAT GAACTATTGG AAGAACAATG GAGCCCCAGC TGAGAAGCTC 
ATTGTTGGAT TCCCAGAGTA TGGACACACC TTCATCCTGA GAAACCCCTC TGATAATGGA 
ATTGGTGCCC CTACCTCTGG TGATGGCCCT GCTGGGCCCT ATACCAGACA GGCTGGGTTC 
TGGGCCTACT ATGAGATTTG CACCTTTCTG AGAAGTGGAG CCACTGAGGT CTGGGATGCC 
TCCCAAGAAG TGCCCTATGC CTATAAGGCC AACGAGTGGC TTGGCTATGA CAATATCAAG 
AGCTTCAGTG TTAAGGCTCA GTGGCTTAAG CAGAACAATT TTGGAGGTGC CATGATCTGG 
GCCATTGACC TTGATGACTT CACTGGCTCT TTCTGTGATC AGGGAAAATT TCCTCTGACT 
TCTACTTTGA ACAAAGCCCT TGGCATATCC ACTGAAGGTT GCACAGCTCC TGACGTGCCT 
TCCGAGCCAG TGACTACTCC TCCAGGAAGT GGGAGTGGGG GTGGAAGCTC CGGAGGAAGC 
TCTGGAGGCA GTGGATTCTG TGCCGACAAA GCAGATGGCC TCTACCCTGT GGCAGATGAC 
AGAAATGCTT TTTGGCAGTG CATCAATGGA ATCACATACC AGCAGCATTG TCAAGCAGGG 
CTTGTTTTTG ATACCAGCTG TAATTGCTGC AACTGGCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mouse acidic mammalian chitinase exhibits transglycosylation activity at somatic tissue pH.
FEBS letters591(20)3310-3318(2017 Oct)
Wakita S,Kobayashi S,Kimura M,Kashimura A,Honda S,Sakaguchi M,Sugahara Y,Kamaya M,Matoska V,Bauer PO,Oyama F

Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease.
Cell169(3)497-509.e13(2017 Apr)
Van Dyken SJ,Liang HE,Naikawadi RP,Woodruff PG,Wolters PJ,Erle DJ,Locksley RM

Loss and Gain of Human Acidic Mammalian Chitinase Activity by Nonsynonymous SNPs.
Molecular biology and evolution33(12)3183-3193(2016 Dec)
Okawa K,Ohno M,Kashimura A,Kimura M,Kobayashi Y,Sakaguchi M,Sugahara Y,Kamaya M,Kino Y,Bauer PO,Oyama F

Acidic chitinase primes the protective immune response to gastrointestinal nematodes.
Nature immunology17(5)538-44(2016 May)
Vannella KM,Ramalingam TR,Hart KM,de Queiroz Prado R,Sciurba J,Barron L,Borthwick LA,Smith AD,Mentink-Kane M,White S,Thompson RW,Cheever AW,Bock K,Moore I,Fitz LJ,Urban JF Jr,Wynn TA

Breast regression protein-39 is not required for experimental autoimmune encephalomyelitis induction.
Clinical immunology (Orlando, Fla.)160(2)133-41(2015 Oct)
Cant? E,Espejo C,Costa C,Montalban X,Comabella M

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Chia1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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