Aacs cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aacs
Entrez Gene ID	78894
Full Name	acetoacetyl-CoA synthetase
Synonyms	2210408B16Rik,SUR5
General protein information	Preferred Names acetoacetyl-CoA synthetase Names acetoacetyl-CoA synthetase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 G1.1

mRNA and Protein(s)

mRNA	Protein	Name
NM_030210.1	NP_084486.1	acetoacetyl-CoA synthetase
NM_030210.2	NP_084486.1	acetoacetyl-CoA synthetase

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Aacs	NP_084486.1
Neurospora crassa	NCU00446	XP_956765.1
Danio rerio (zebrafish)	aacs	NP_957303.1
Caenorhabditis elegans (roundworm)	sur-5	NP_509229.1
Magnaporthe oryzae (rice blast fungus)	MGG_05196	XP_003712739.1
Canis lupus familiaris (dog)	AACS	XP_543365.3
Xenopus tropicalis (tropical clawed frog)	aacs	NP_001039244.1
Homo sapiens (human)	AACS	NP_076417.2
	AACS	XP_001103579.1
Rattus norvegicus (Norway rat)	Aacs	NP_075592.1
Pan troglodytes (chimpanzee)	AACS	XP_001135792.2
Bos taurus (cattle)	AACS	NP_001157401.1
Gallus gallus (chicken)	AACS	NP_001006184.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells.
Hasegawa S, Imai M, Yamasaki M, Takahashi N, Fukui T
Biochemical and biophysical research communications495(1)652-658(2018 01)

Site-specific cleavage of acetoacetyl-CoA synthetase by legumain.
Hasegawa S, Inoue D, Yamasaki M, Li C, Imai M, Takahashi N, Fukui T
FEBS letters590(11)1592-601(2016 Jun)

Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP-2 and affects serum cholesterol levels.
Hasegawa S, Noda K, Maeda A, Matsuoka M, Yamasaki M, Fukui T
Molecular genetics and metabolism107(3)553-60(2012 Nov)

Acetoacetyl-CoA synthetase is essential for normal neuronal development.
Hasegawa S, Kume H, Iinuma S, Yamasaki M, Takahashi N, Fukui T
Biochemical and biophysical research communications427(2)398-403(2012 Oct)

The role of acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, in 3T3-L1 adipocyte differentiation.
Hasegawa S, Ikeda Y, Yamasaki M, Fukui T
Biological & pharmaceutical bulletin35(11)1980-5(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that Sp1 regulates gene expression of AACS in Neuro-2a cells and ketone body utilization affects the balance of histone acetylation.
Title: Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells.

Cleavage of AACS by legumain is critical for the regulation of enzymatic activity and results in gain-of-function changes.
Title: Site-specific cleavage of acetoacetyl-CoA synthetase by legumain.

Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP-2 and affects serum cholesterol levels.
Title: Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP-2 and affects serum cholesterol levels.

These results suggest that AACS is regulated by SREBP-2 and involves in the normal development of neurons.
Title: Acetoacetyl-CoA synthetase is essential for normal neuronal development.

Knockdown of AACS inhibits differentiation of 3T3-L1 adipocytes and suppresses expression of the adipocyte markers, peroxisome proliferator-activated receptor gama and CCAAT/enhancer binding protein alpha.
Title: The role of acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, in 3T3-L1 adipocyte differentiation.

The following Aacs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aacs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08103	NM_030210.2 Latest version!	Mus musculus acetoacetyl-CoA synthetase (Aacs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
View Old Accession Versions >>
OMu08103	NM_030210.1	Mus musculus acetoacetyl-CoA synthetase (Aacs), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aacs ( NM_030210.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084486.1 Mus musculus acetoacetyl-CoA synthetase (Aacs), mRNA.

Price & Availability↑

CloneID	OMu08103
Clone ID Related Accession (Same CDS sequence)	NM_030210.1 , NM_030210.2
Accession Version	NM_030210.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-31
Organism	Mus musculus(house mouse)
Product	acetoacetyl-CoA synthetase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK019063.1. ##Evidence-Data-START## Transcript exon combination :: AK019063.1, AK044017.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAAGC TGGCGCGGCT CGAGCGCGAG GAGATCATGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGGACAC GCAGATGGAC CGCTTCCGGG CGGCCGTGGG TACCGCCTGC 
GGCCTGGCGC TTGGGAATTA CAATGACTTA TACCACTGGT CTGTCCGGTC GTATATGGAC 
TTTTGGGCTG AGTTCTGGAA GTTCAGTGGA ATCGTCTACT CACGCATGTA TGATGAGGTT 
GTGGACACAT CCAAAGGGAT TGCAGATGTC CCCGAGTGGT TCAGAGGCAG CCGCCTCAAC 
TACGCAGAGA ACCTCCTGCG GCACAAGGAG AACGACAGAG TCGCCCTTTA CGTGGCCCGG 
GAAGGCAGAG AGGAGATCGT GAAGGTGACT TTTGAAGAGC TGCGGCAGCA GGTGGCTCTG 
TTCGCAGCTG CCATGAGGAA GATGGGCGTG AAGAAAGGGG ACCGTGTGGT CGGCTATCTA 
CCCAACAGTG CGCATGCGGT GGAGGCCATG CTGGCTGCTG CCAGTATTGG GGCCATTTGG 
AGTTCTACCT CGCCGGACTT TGGTGTAAAT GGTGTCCTGG ACCGCTTTTC TCAAATTCAG 
CCGAAACTTA TCTTCTCGGT GGAGGCTGTT GTCTACAATG GCAAGGAGCA TGGTCACCTG 
GAGAAGCTGC AGCGAGTTGT GAAAGGACTG CCTGACCTGC AGCGAGTGGT GCTGATCCCC 
TATGTCCTCC CAAGGGAGAA GATTGACATT TCCAAGATCC CCAACAGTGT GTTCCTGGAT 
GATTTCCTGG CAAGCGGGAC CGGCGCGCAG GCGCCGCAGC TCGAGTTTGA GCAGCTGCCC 
TTCAGCCACC CTCTGTTCAT CATGTTCTCC TCAGGCACGA CGGGAGCGCC CAAGTGCATG 
GTGCACTCAG CCGGGGGCAC CCTCATCCAG CACCTGAAGG AGCACATGCT ACACGGCAAC 
ATGACAAGCA GTGACATCCT GCTCTACTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCAG CCCTGGCCAC AGGAGCATCC TTGGTCCTGT ATGATGGCTC CCCGCTGGTT 
CCGACCCCCA ACGTGTTGTG GGACCTTGTG GACAGGATAG GGATCACCAT CCTGGGAACG 
GGAGCCAAGT GGCTGTCAGT GCTGGAGGAG AAGGACATGA AGCCAGTGGA AACTCACAAC 
CTCCACACGC TGCACACGAT CCTGTCCACC GGCTCGCCGC TGAAAGCCCA GAGTTACGAG 
TATGTGTACA GATGCATCAA GAGCTCCGTG CTCCTGGGCT CCATCTCAGG AGGCACTGAC 
ATCATCTCCT GTTTCATGGG CCAGAACTCC TCTATTCCTG TGTACAAGGG TGAGATCCAA 
GCCCGGAACC TTGGCATGGC TGTGGAAGCC TGGGACGAGG AAGGGAAGGC CGTCTGGGGA 
GCGAGTGGCG AGCTGGTGTG CACTAAGCCC ATTCCCTGCC AGCCCACGCA CTTCTGGAAC 
GACGAGAACG GCAGCAAGTA CCGGAAGGCT TACTTCTCCA AATTCCCAGG TGTCTGGGCA 
CACGGTGACT ACTGCAGGAT CAACCCCAAA ACAGGAGGCA TTATCATGCT GGGCCGTAGT 
GATGGCACCC TCAACCCCAA TGGCGTCCGC TTTGGCAGCT CGGAGATCTA CAACATCGTG 
GAAGCCTTCG ATGAGGTGGA GGACAGCCTG TGTGTACCCC AGTACAACAG AGATGGCGAG 
GAGCGGGTGG TCCTGTTCCT GAAGATGGCG TCCGGGCACA CTTTCCAGCC TGACCTCGTG 
AAGCGCATCC GAGACGCCAT CCGACTTGGC CTGTCTGCCC GCCATGTGCC CAGCCTCATC 
CTGGAGACCC GAGGCATTCC ATACACACTC AATGGCAAGA AAGTGGAGGT GGCCGTGAAG 
CAGGTGATGG CTGGGAGGAC TGTGGAGCAC CGGGGGGCCT TCTCCAACCC CGAGACCCTC 
GACCTGTACC GGGACATCCC TGAGCTGCAG GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084486.1
CDS	106..2124
Translation	MSKLARLEREEIMECQVMWEPDSKKDTQMDRFRAAVGTACGLALGNYNDLYHWSVRSYMDFWAEFWKFSGIVYSRMYDEVVDTSKGIADVPEWFRGSRLNYAENLLRHKENDRVALYVAREGREEIVKVTFEELRQQVALFAAAMRKMGVKKGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHGHLEKLQRVVKGLPDLQRVVLIPYVLPREKIDISKIPNSVFLDDFLASGTGAQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHMLHGNMTSSDILLYYTTVGWMMWNWMVSALATGASLVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMKPVETHNLHTLHTILSTGSPLKAQSYEYVYRCIKSSVLLGSISGGTDIISCFMGQNSSIPVYKGEIQARNLGMAVEAWDEEGKAVWGASGELVCTKPIPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIIMLGRSDGTLNPNGVRFGSSEIYNIVEAFDEVEDSLCVPQYNRDGEERVVLFLKMASGHTFQPDLVKRIRDAIRLGLSARHVPSLILETRGIPYTLNGKKVEVAVKQVMAGRTVEHRGAFSNPETLDLYRDIPELQDF

Target ORF information:

RefSeq Version	NM_030210.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus acetoacetyl-CoA synthetase (Aacs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030210.1

ORF Insert Sequence:

ATGTCCAAGC TGGCGCGGCT CGAGCGCGAG GAGATCATGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGGACAC GCAGATGGAC CGCTTCCGGG CGGCCGTGGG TACCGCCTGC 
GGCCTGGCGC TTGGGAATTA CAATGACTTA TACCACTGGT CTGTCCGGTC GTATATGGAC 
TTTTGGGCTG AGTTCTGGAA GTTCAGTGGA ATCGTCTACT CACGCATGTA TGATGAGGTT 
GTGGACACAT CCAAAGGGAT TGCAGATGTC CCCGAGTGGT TCAGAGGCAG CCGCCTCAAC 
TACGCAGAGA ACCTCCTGCG GCACAAGGAG AACGACAGAG TCGCCCTTTA CGTGGCCCGG 
GAAGGCAGAG AGGAGATCGT GAAGGTGACT TTTGAAGAGC TGCGGCAGCA GGTGGCTCTG 
TTCGCAGCTG CCATGAGGAA GATGGGCGTG AAGAAAGGGG ACCGTGTGGT CGGCTATCTA 
CCCAACAGTG CGCATGCGGT GGAGGCCATG CTGGCTGCTG CCAGTATTGG GGCCATTTGG 
AGTTCTACCT CGCCGGACTT TGGTGTAAAT GGTGTCCTGG ACCGCTTTTC TCAAATTCAG 
CCGAAACTTA TCTTCTCGGT GGAGGCTGTT GTCTACAATG GCAAGGAGCA TGGTCACCTG 
GAGAAGCTGC AGCGAGTTGT GAAAGGACTG CCTGACCTGC AGCGAGTGGT GCTGATCCCC 
TATGTCCTCC CAAGGGAGAA GATTGACATT TCCAAGATCC CCAACAGTGT GTTCCTGGAT 
GATTTCCTGG CAAGCGGGAC CGGCGCGCAG GCGCCGCAGC TCGAGTTTGA GCAGCTGCCC 
TTCAGCCACC CTCTGTTCAT CATGTTCTCC TCAGGCACGA CGGGAGCGCC CAAGTGCATG 
GTGCACTCAG CCGGGGGCAC CCTCATCCAG CACCTGAAGG AGCACATGCT ACACGGCAAC 
ATGACAAGCA GTGACATCCT GCTCTACTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCAG CCCTGGCCAC AGGAGCATCC TTGGTCCTGT ATGATGGCTC CCCGCTGGTT 
CCGACCCCCA ACGTGTTGTG GGACCTTGTG GACAGGATAG GGATCACCAT CCTGGGAACG 
GGAGCCAAGT GGCTGTCAGT GCTGGAGGAG AAGGACATGA AGCCAGTGGA AACTCACAAC 
CTCCACACGC TGCACACGAT CCTGTCCACC GGCTCGCCGC TGAAAGCCCA GAGTTACGAG 
TATGTGTACA GATGCATCAA GAGCTCCGTG CTCCTGGGCT CCATCTCAGG AGGCACTGAC 
ATCATCTCCT GTTTCATGGG CCAGAACTCC TCTATTCCTG TGTACAAGGG TGAGATCCAA 
GCCCGGAACC TTGGCATGGC TGTGGAAGCC TGGGACGAGG AAGGGAAGGC CGTCTGGGGA 
GCGAGTGGCG AGCTGGTGTG CACTAAGCCC ATTCCCTGCC AGCCCACGCA CTTCTGGAAC 
GACGAGAACG GCAGCAAGTA CCGGAAGGCT TACTTCTCCA AATTCCCAGG TGTCTGGGCA 
CACGGTGACT ACTGCAGGAT CAACCCCAAA ACAGGAGGCA TTATCATGCT GGGCCGTAGT 
GATGGCACCC TCAACCCCAA TGGCGTCCGC TTTGGCAGCT CGGAGATCTA CAACATCGTG 
GAAGCCTTCG ATGAGGTGGA GGACAGCCTG TGTGTACCCC AGTACAACAG AGATGGCGAG 
GAGCGGGTGG TCCTGTTCCT GAAGATGGCG TCCGGGCACA CTTTCCAGCC TGACCTCGTG 
AAGCGCATCC GAGACGCCAT CCGACTTGGC CTGTCTGCCC GCCATGTGCC CAGCCTCATC 
CTGGAGACCC GAGGCATTCC ATACACACTC AATGGCAAGA AAGTGGAGGT GGCCGTGAAG 
CAGGTGATGG CTGGGAGGAC TGTGGAGCAC CGGGGGGCCT TCTCCAACCC CGAGACCCTC 
GACCTGTACC GGGACATCCC TGAGCTGCAG GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aacs ( NM_030210.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084486.1 Mus musculus acetoacetyl-CoA synthetase (Aacs), mRNA.

Price & Availability↑

CloneID	OMu08103
Clone ID Related Accession (Same CDS sequence)	NM_030210.1 , NM_030210.2
Accession Version	NM_030210.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Mus musculus(house mouse)
Product	acetoacetyl-CoA synthetase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119268.12. On Aug 16, 2018 this sequence version replaced NM_030210.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AK044017.1, AK165418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAAGC TGGCGCGGCT CGAGCGCGAG GAGATCATGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGGACAC GCAGATGGAC CGCTTCCGGG CGGCCGTGGG TACCGCCTGC 
GGCCTGGCGC TTGGGAATTA CAATGACTTA TACCACTGGT CTGTCCGGTC GTATATGGAC 
TTTTGGGCTG AGTTCTGGAA GTTCAGTGGA ATCGTCTACT CACGCATGTA TGATGAGGTT 
GTGGACACAT CCAAAGGGAT TGCAGATGTC CCCGAGTGGT TCAGAGGCAG CCGCCTCAAC 
TACGCAGAGA ACCTCCTGCG GCACAAGGAG AACGACAGAG TCGCCCTTTA CGTGGCCCGG 
GAAGGCAGAG AGGAGATCGT GAAGGTGACT TTTGAAGAGC TGCGGCAGCA GGTGGCTCTG 
TTCGCAGCTG CCATGAGGAA GATGGGCGTG AAGAAAGGGG ACCGTGTGGT CGGCTATCTA 
CCCAACAGTG CGCATGCGGT GGAGGCCATG CTGGCTGCTG CCAGTATTGG GGCCATTTGG 
AGTTCTACCT CGCCGGACTT TGGTGTAAAT GGTGTCCTGG ACCGCTTTTC TCAAATTCAG 
CCGAAACTTA TCTTCTCGGT GGAGGCTGTT GTCTACAATG GCAAGGAGCA TGGTCACCTG 
GAGAAGCTGC AGCGAGTTGT GAAAGGACTG CCTGACCTGC AGCGAGTGGT GCTGATCCCC 
TATGTCCTCC CAAGGGAGAA GATTGACATT TCCAAGATCC CCAACAGTGT GTTCCTGGAT 
GATTTCCTGG CAAGCGGGAC CGGCGCGCAG GCGCCGCAGC TCGAGTTTGA GCAGCTGCCC 
TTCAGCCACC CTCTGTTCAT CATGTTCTCC TCAGGCACGA CGGGAGCGCC CAAGTGCATG 
GTGCACTCAG CCGGGGGCAC CCTCATCCAG CACCTGAAGG AGCACATGCT ACACGGCAAC 
ATGACAAGCA GTGACATCCT GCTCTACTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCAG CCCTGGCCAC AGGAGCATCC TTGGTCCTGT ATGATGGCTC CCCGCTGGTT 
CCGACCCCCA ACGTGTTGTG GGACCTTGTG GACAGGATAG GGATCACCAT CCTGGGAACG 
GGAGCCAAGT GGCTGTCAGT GCTGGAGGAG AAGGACATGA AGCCAGTGGA AACTCACAAC 
CTCCACACGC TGCACACGAT CCTGTCCACC GGCTCGCCGC TGAAAGCCCA GAGTTACGAG 
TATGTGTACA GATGCATCAA GAGCTCCGTG CTCCTGGGCT CCATCTCAGG AGGCACTGAC 
ATCATCTCCT GTTTCATGGG CCAGAACTCC TCTATTCCTG TGTACAAGGG TGAGATCCAA 
GCCCGGAACC TTGGCATGGC TGTGGAAGCC TGGGACGAGG AAGGGAAGGC CGTCTGGGGA 
GCGAGTGGCG AGCTGGTGTG CACTAAGCCC ATTCCCTGCC AGCCCACGCA CTTCTGGAAC 
GACGAGAACG GCAGCAAGTA CCGGAAGGCT TACTTCTCCA AATTCCCAGG TGTCTGGGCA 
CACGGTGACT ACTGCAGGAT CAACCCCAAA ACAGGAGGCA TTATCATGCT GGGCCGTAGT 
GATGGCACCC TCAACCCCAA TGGCGTCCGC TTTGGCAGCT CGGAGATCTA CAACATCGTG 
GAAGCCTTCG ATGAGGTGGA GGACAGCCTG TGTGTACCCC AGTACAACAG AGATGGCGAG 
GAGCGGGTGG TCCTGTTCCT GAAGATGGCG TCCGGGCACA CTTTCCAGCC TGACCTCGTG 
AAGCGCATCC GAGACGCCAT CCGACTTGGC CTGTCTGCCC GCCATGTGCC CAGCCTCATC 
CTGGAGACCC GAGGCATTCC ATACACACTC AATGGCAAGA AAGTGGAGGT GGCCGTGAAG 
CAGGTGATGG CTGGGAGGAC TGTGGAGCAC CGGGGGGCCT TCTCCAACCC CGAGACCCTC 
GACCTGTACC GGGACATCCC TGAGCTGCAG GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084486.1
CDS	105..2123
Translation	MSKLARLEREEIMECQVMWEPDSKKDTQMDRFRAAVGTACGLALGNYNDLYHWSVRSYMDFWAEFWKFSGIVYSRMYDEVVDTSKGIADVPEWFRGSRLNYAENLLRHKENDRVALYVAREGREEIVKVTFEELRQQVALFAAAMRKMGVKKGDRVVGYLPNSAHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHGHLEKLQRVVKGLPDLQRVVLIPYVLPREKIDISKIPNSVFLDDFLASGTGAQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHMLHGNMTSSDILLYYTTVGWMMWNWMVSALATGASLVLYDGSPLVPTPNVLWDLVDRIGITILGTGAKWLSVLEEKDMKPVETHNLHTLHTILSTGSPLKAQSYEYVYRCIKSSVLLGSISGGTDIISCFMGQNSSIPVYKGEIQARNLGMAVEAWDEEGKAVWGASGELVCTKPIPCQPTHFWNDENGSKYRKAYFSKFPGVWAHGDYCRINPKTGGIIMLGRSDGTLNPNGVRFGSSEIYNIVEAFDEVEDSLCVPQYNRDGEERVVLFLKMASGHTFQPDLVKRIRDAIRLGLSARHVPSLILETRGIPYTLNGKKVEVAVKQVMAGRTVEHRGAFSNPETLDLYRDIPELQDF

Target ORF information:

RefSeq Version	NM_030210.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus acetoacetyl-CoA synthetase (Aacs), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030210.2

ORF Insert Sequence:

ATGTCCAAGC TGGCGCGGCT CGAGCGCGAG GAGATCATGG AGTGCCAGGT GATGTGGGAG 
CCTGACAGCA AGAAGGACAC GCAGATGGAC CGCTTCCGGG CGGCCGTGGG TACCGCCTGC 
GGCCTGGCGC TTGGGAATTA CAATGACTTA TACCACTGGT CTGTCCGGTC GTATATGGAC 
TTTTGGGCTG AGTTCTGGAA GTTCAGTGGA ATCGTCTACT CACGCATGTA TGATGAGGTT 
GTGGACACAT CCAAAGGGAT TGCAGATGTC CCCGAGTGGT TCAGAGGCAG CCGCCTCAAC 
TACGCAGAGA ACCTCCTGCG GCACAAGGAG AACGACAGAG TCGCCCTTTA CGTGGCCCGG 
GAAGGCAGAG AGGAGATCGT GAAGGTGACT TTTGAAGAGC TGCGGCAGCA GGTGGCTCTG 
TTCGCAGCTG CCATGAGGAA GATGGGCGTG AAGAAAGGGG ACCGTGTGGT CGGCTATCTA 
CCCAACAGTG CGCATGCGGT GGAGGCCATG CTGGCTGCTG CCAGTATTGG GGCCATTTGG 
AGTTCTACCT CGCCGGACTT TGGTGTAAAT GGTGTCCTGG ACCGCTTTTC TCAAATTCAG 
CCGAAACTTA TCTTCTCGGT GGAGGCTGTT GTCTACAATG GCAAGGAGCA TGGTCACCTG 
GAGAAGCTGC AGCGAGTTGT GAAAGGACTG CCTGACCTGC AGCGAGTGGT GCTGATCCCC 
TATGTCCTCC CAAGGGAGAA GATTGACATT TCCAAGATCC CCAACAGTGT GTTCCTGGAT 
GATTTCCTGG CAAGCGGGAC CGGCGCGCAG GCGCCGCAGC TCGAGTTTGA GCAGCTGCCC 
TTCAGCCACC CTCTGTTCAT CATGTTCTCC TCAGGCACGA CGGGAGCGCC CAAGTGCATG 
GTGCACTCAG CCGGGGGCAC CCTCATCCAG CACCTGAAGG AGCACATGCT ACACGGCAAC 
ATGACAAGCA GTGACATCCT GCTCTACTAC ACCACGGTCG GCTGGATGAT GTGGAACTGG 
ATGGTGTCAG CCCTGGCCAC AGGAGCATCC TTGGTCCTGT ATGATGGCTC CCCGCTGGTT 
CCGACCCCCA ACGTGTTGTG GGACCTTGTG GACAGGATAG GGATCACCAT CCTGGGAACG 
GGAGCCAAGT GGCTGTCAGT GCTGGAGGAG AAGGACATGA AGCCAGTGGA AACTCACAAC 
CTCCACACGC TGCACACGAT CCTGTCCACC GGCTCGCCGC TGAAAGCCCA GAGTTACGAG 
TATGTGTACA GATGCATCAA GAGCTCCGTG CTCCTGGGCT CCATCTCAGG AGGCACTGAC 
ATCATCTCCT GTTTCATGGG CCAGAACTCC TCTATTCCTG TGTACAAGGG TGAGATCCAA 
GCCCGGAACC TTGGCATGGC TGTGGAAGCC TGGGACGAGG AAGGGAAGGC CGTCTGGGGA 
GCGAGTGGCG AGCTGGTGTG CACTAAGCCC ATTCCCTGCC AGCCCACGCA CTTCTGGAAC 
GACGAGAACG GCAGCAAGTA CCGGAAGGCT TACTTCTCCA AATTCCCAGG TGTCTGGGCA 
CACGGTGACT ACTGCAGGAT CAACCCCAAA ACAGGAGGCA TTATCATGCT GGGCCGTAGT 
GATGGCACCC TCAACCCCAA TGGCGTCCGC TTTGGCAGCT CGGAGATCTA CAACATCGTG 
GAAGCCTTCG ATGAGGTGGA GGACAGCCTG TGTGTACCCC AGTACAACAG AGATGGCGAG 
GAGCGGGTGG TCCTGTTCCT GAAGATGGCG TCCGGGCACA CTTTCCAGCC TGACCTCGTG 
AAGCGCATCC GAGACGCCAT CCGACTTGGC CTGTCTGCCC GCCATGTGCC CAGCCTCATC 
CTGGAGACCC GAGGCATTCC ATACACACTC AATGGCAAGA AAGTGGAGGT GGCCGTGAAG 
CAGGTGATGG CTGGGAGGAC TGTGGAGCAC CGGGGGGCCT TCTCCAACCC CGAGACCCTC 
GACCTGTACC GGGACATCCC TGAGCTGCAG GACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcriptional regulation of acetoacetyl-CoA synthetase by Sp1 in neuroblastoma cells.
Biochemical and biophysical research communications495(1)652-658(2018 01)
Hasegawa S,Imai M,Yamasaki M,Takahashi N,Fukui T

Site-specific cleavage of acetoacetyl-CoA synthetase by legumain.
FEBS letters590(11)1592-601(2016 Jun)
Hasegawa S,Inoue D,Yamasaki M,Li C,Imai M,Takahashi N,Fukui T

Acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, is controlled by SREBP-2 and affects serum cholesterol levels.
Molecular genetics and metabolism107(3)553-60(2012 Nov)
Hasegawa S,Noda K,Maeda A,Matsuoka M,Yamasaki M,Fukui T

Acetoacetyl-CoA synthetase is essential for normal neuronal development.
Biochemical and biophysical research communications427(2)398-403(2012 Oct)
Hasegawa S,Kume H,Iinuma S,Yamasaki M,Takahashi N,Fukui T

The role of acetoacetyl-CoA synthetase, a ketone body-utilizing enzyme, in 3T3-L1 adipocyte differentiation.
Biological & pharmaceutical bulletin35(11)1980-5(2012)
Hasegawa S,Ikeda Y,Yamasaki M,Fukui T