Vsig1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vsig1
Entrez Gene ID	78789
Full Name	V-set and immunoglobulin domain containing 1
Synonyms	1700062D20Rik,4930405J24Rik
General protein information	Preferred Names V-set and immunoglobulin domain containing 1 Names V-set and immunoglobulin domain-containing protein 1 cell surface A33 antigen glycoprotein A34
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X F1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006528630.3	XP_006528693.1	V-set and immunoglobulin domain-containing protein 1 isoform X1
NM_001359143.1	NP_001346072.1	V-set and immunoglobulin domain-containing protein 1 isoform 3 precursor
NM_026103.1	NP_080379.1	V-set and immunoglobulin domain-containing protein 1 isoform 2
XM_006528631.3	XP_006528694.1	V-set and immunoglobulin domain-containing protein 1 isoform X2
XM_006528631.2	XP_006528694.1	V-set and immunoglobulin domain-containing protein 1 isoform X1
NM_030181.3	NP_084457.2	V-set and immunoglobulin domain-containing protein 1 isoform 1 precursor
XM_006528631.4	XP_006528694.1	V-set and immunoglobulin domain-containing protein 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	VSIG1	XP_005641711.1
Bos taurus (cattle)	VSIG1	NP_001039395.1
Mus musculus (house mouse)	Vsig1	NP_084457.2
Rattus norvegicus (Norway rat)	Vsig1	NP_001032873.1
Homo sapiens (human)	VSIG1	NP_001164024.1
Pan troglodytes (chimpanzee)	VSIG1	XP_521214.3
Gallus gallus (chicken)	VSIG1	NP_001001745.1
Xenopus tropicalis (tropical clawed frog)	vsig1	NP_001106380.1
	VSIG1	XP_001092052.2

Gene Ontology
Bibliography

Related articles in PubMed

The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis.
Herriges MJ, Tischfield DJ, Cui Z, Morley MP, Han Y, Babu A, Li S, Lu M, Cendan I, Garcia BA, Anderson SA, Morrisey EE
Genes & development31(9)889-903(2017 May)

Female-to-male sex reversal in mice caused by transgenic overexpression of Dmrt1.
Zhao L, Svingen T, Ng ET, Koopman P
Development (Cambridge, England)142(6)1083-8(2015 Mar)

Impairments to the GH-IGF-I axis in hSOD1G93A mice give insight into possible mechanisms of GH dysregulation in patients with amyotrophic lateral sclerosis.
Steyn FJ, Ngo ST, Lee JD, Leong JW, Buckley AJ, Veldhuis JD, McCombe PA, Chen C, Bellingham MC
Endocrinology153(8)3735-46(2012 Aug)

Adhesion protein VSIG1 is required for the proper differentiation of glandular gastric epithelia.
Oidovsambuu O, Nyamsuren G, Liu S, G?ring W, Engel W, Adham IM
PloS one6(10)e25908(2011)

Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1.
Kim E, Lee Y, Kim JS, Song BS, Kim SU, Huh JW, Lee SR, Kim SH, Hong Y, Chang KT
Molecules and cells30(5)443-8(2010 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VSIG1 is required for the establishment of glandular versus squamous epithelia in the stomach.
Title: Adhesion protein VSIG1 is required for the proper differentiation of glandular gastric epithelia.

mouse VSIG1 interacts with Sertoli cells by heterophilic adhesion via its first Ig-like domain
Title: Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1.

The following Vsig1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vsig1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01573	NM_026103.1 Latest version!	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu01573	XM_006528631.4 Latest version!	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu08035	NM_030181.3 Latest version!	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00
OMu56555	NM_001359143.1 Latest version!	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu56555	XM_006528630.3 Latest version!	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OMu01573	XM_006528631.2	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OMu01573	XM_006528631.3	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vsig1 ( NM_026103.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080379.1 Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01573
Clone ID Related Accession (Same CDS sequence)	NM_026103.1 , XM_006528631.3 , XM_006528631.2 , XM_006528631.4
Accession Version	NM_026103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	V-set and immunoglobulin domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK006862.1 and AK132956.1. Transcript Variant: This variant (2) contains a different segment for its 5' UTR and lacks part of the 5' coding region with the use of a downstream start codon, compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus when it is compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK006862.1, BC048589.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080379.1
CDS	164..1066
Translation	MQPADSGIYICDVNNPPDFVGKNQGLLDVTVLVKPSKPFCTIQGRPEAGHPISLSCLSAFGTPSPLYYWYKIEGNTIVPVKESFNTATGVLVIGNLTNFEQGYYQCTAINSLGNSSCEIDLTSSHPEVGIIIGALVGALIGAAVIICVVYFARNKVKSKQQKNLNSSTELEPMTKVHHPQQSEAISADGVQLEGTLPSSIHAGHNTEPTTTAVLEPEYEPNPPLETTTQPDPEPEGSVPVLAPEAEIQPHPELDPETETEPEPEPEPKPEPEPEPELEPDPQSGVIIEPLSKAGEDTVKA

Target ORF information:

RefSeq Version	NM_026103.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026103.1

ORF Insert Sequence:

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vsig1 ( XM_006528631.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528694.1 Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01573
Clone ID Related Accession (Same CDS sequence)	NM_026103.1 , XM_006528631.3 , XM_006528631.2 , XM_006528631.4
Accession Version	XM_006528631.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	V-set and immunoglobulin domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528631.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528694.1
CDS	301..1203
Translation	MQPADSGIYICDVNNPPDFVGKNQGLLDVTVLVKPSKPFCTIQGRPEAGHPISLSCLSAFGTPSPLYYWYKIEGNTIVPVKESFNTATGVLVIGNLTNFEQGYYQCTAINSLGNSSCEIDLTSSHPEVGIIIGALVGALIGAAVIICVVYFARNKVKSKQQKNLNSSTELEPMTKVHHPQQSEAISADGVQLEGTLPSSIHAGHNTEPTTTAVLEPEYEPNPPLETTTQPDPEPEGSVPVLAPEAEIQPHPELDPETETEPEPEPEPKPEPEPEPELEPDPQSGVIIEPLSKAGEDTVKA

Target ORF information:

RefSeq Version	XM_006528631.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528631.3

ORF Insert Sequence:

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vsig1 ( XM_006528631.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528694.1 Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01573
Clone ID Related Accession (Same CDS sequence)	NM_026103.1 , XM_006528631.3 , XM_006528631.2 , XM_006528631.4
Accession Version	XM_006528631.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	V-set and immunoglobulin domain-containing protein 1 isoform X1
Comment

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528694.1
Translation	MQPADSGIYICDVNNPPDFVGKNQGLLDVTVLVKPSKPFCTIQGRPEAGHPISLSCLSAFGTPSPLYYWYKIEGNTIVPVKESFNTATGVLVIGNLTNFEQGYYQCTAINSLGNSSCEIDLTSSHPEVGIIIGALVGALIGAAVIICVVYFARNKVKSKQQKNLNSSTELEPMTKVHHPQQSEAISADGVQLEGTLPSSIHAGHNTEPTTTAVLEPEYEPNPPLETTTQPDPEPEGSVPVLAPEAEIQPHPELDPETETEPEPEPEPKPEPEPEPELEPDPQSGVIIEPLSKAGEDTVKA

Target ORF information:

RefSeq Version	XM_006528631.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528631.2

ORF Insert Sequence:

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vsig1 ( XM_006528631.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528694.1 Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu01573
Clone ID Related Accession (Same CDS sequence)	NM_026103.1 , XM_006528631.3 , XM_006528631.2 , XM_006528631.4
Accession Version	XM_006528631.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	V-set and immunoglobulin domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528631.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528694.1
CDS	302..1204
Translation	MQPADSGIYICDVNNPPDFVGKNQGLLDVTVLVKPSKPFCTIQGRPEAGHPISLSCLSAFGTPSPLYYWYKIEGNTIVPVKESFNTATGVLVIGNLTNFEQGYYQCTAINSLGNSSCEIDLTSSHPEVGIIIGALVGALIGAAVIICVVYFARNKVKSKQQKNLNSSTELEPMTKVHHPQQSEAISADGVQLEGTLPSSIHAGHNTEPTTTAVLEPEYEPNPPLETTTQPDPEPEGSVPVLAPEAEIQPHPELDPETETEPEPEPEPKPEPEPEPELEPDPQSGVIIEPLSKAGEDTVKA

Target ORF information:

RefSeq Version	XM_006528631.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528631.4

ORF Insert Sequence:

ATGCAGCCAG CAGACAGTGG AATTTACATC TGTGATGTCA ACAATCCTCC AGATTTTGTG 
GGCAAAAACC AAGGCCTCCT TGACGTCACT GTCTTAGTCA AACCTTCCAA GCCCTTTTGT 
ACCATCCAAG GAAGACCAGA AGCAGGCCAT CCTATTTCCT TGTCTTGCCT TTCTGCCTTT 
GGAACACCGT CTCCTTTGTA TTACTGGTAT AAGATTGAGG GGAACACCAT TGTGCCAGTA 
AAAGAAAGTT TCAACACGGC CACTGGAGTT TTAGTTATTG GAAATCTAAC AAATTTTGAA 
CAAGGTTATT ACCAGTGTAC TGCCATCAAC AGTCTTGGCA ATAGTTCCTG TGAAATTGAC 
TTAACGTCTT CACATCCAGA AGTTGGGATC ATCATCGGAG CTTTGGTCGG TGCCCTGATA 
GGAGCTGCTG TTATCATCTG TGTGGTGTAC TTTGCCAGGA ACAAAGTTAA ATCCAAGCAG 
CAGAAGAATT TAAATTCCAG CACAGAACTT GAGCCAATGA CAAAGGTACA CCATCCCCAA 
CAAAGTGAGG CAATTTCAGC TGATGGCGTC CAGCTAGAAG GAACTCTGCC ATCTTCCATC 
CATGCTGGCC ACAACACCGA ACCTACTACT ACAGCAGTCT TGGAGCCAGA ATATGAGCCT 
AATCCTCCAC TGGAAACTAC CACACAGCCT GACCCTGAAC CAGAGGGTTC AGTGCCAGTG 
CTAGCACCTG AGGCAGAGAT TCAACCACAC CCAGAGCTGG ACCCGGAGAC TGAGACAGAG 
CCTGAGCCTG AGCCTGAGCC TAAGCCTGAG CCTGAGCCGG AACCTGAGCT TGAGCCTGAT 
CCCCAGTCTG GAGTAATAAT TGAGCCTCTG AGCAAGGCGG GAGAGGATAC AGTTAAGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vsig1 ( NM_030181.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_084457.2 Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu08035
Clone ID Related Accession (Same CDS sequence)	NM_030181.3
Accession Version	NM_030181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	V-set and immunoglobulin domain-containing protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132956.1. On Jan 7, 2010 this sequence version replaced NM_030181.2. Transcript Variant: This variant (1) represents the longer transcript. It encodes the longer protein (isoform 1). ##Evidence-Data-START## Transcript exon combination :: AK132956.1, AK136562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGTGT TTGCATTTTG GAAGGTCTTT CTGATCTTAA ACTGCCTTGC AGGTCAGGTC 
AGTATGGTGC AAGTGACCAT CCCAGACACT TTCGTGAATG TGACTGTTGG ATCTAATGTT 
ACTCTTCTCT GCCTCTATAC CACCACCGAG AAGTCCCTGG AAAAGCTTTC AATTCAATGG 
TCTTTCTTCC ACAATAAGGA AATGGAGGAG CCAATATCTA TCTACTACTC TGAAGGTGGA 
CAGGCTTCAG CTATTGGGCA ATTCAAAGAC CGAATAATTG GGGCTACTAA TCCCGGTAAT 
GCATCTATCA CCATATTGCA TATGCAGCCA GCAGACAGTG GAATTTACAT CTGTGATGTC 
AACAATCCTC CAGATTTTGT GGGCAAAAAC CAAGGCCTCC TTGACGTCAC TGTCTTAGTC 
AAACCTTCCA AGCCCTTTTG TACCATCCAA GGAAGACCAG AAGCAGGCCA TCCTATTTCC 
TTGTCTTGCC TTTCTGCCTT TGGAACACCG TCTCCTTTGT ATTACTGGTA TAAGATTGAG 
GGGAACACCA TTGTGCCAGT AAAAGAAAGT TTCAACACGG CCACTGGAGT TTTAGTTATT 
GGAAATCTAA CAAATTTTGA ACAAGGTTAT TACCAGTGTA CTGCCATCAA CAGTCTTGGC 
AATAGTTCCT GTGAAATTGA CTTAACGTCT TCACATCCAG AAGTTGGGAT CATCATCGGA 
GCTTTGGTCG GTGCCCTGAT AGGAGCTGCT GTTATCATCT GTGTGGTGTA CTTTGCCAGG 
AACAAAGTTA AATCCAAGCA GCAGAAGAAT TTAAATTCCA GCACAGAACT TGAGCCAATG 
ACAAAGGTAC ACCATCCCCA ACAAAGTGAG GCAATTTCAG CTGATGGCGT CCAGCTAGAA 
GGAACTCTGC CATCTTCCAT CCATGCTGGC CACAACACCG AACCTACTAC TACAGCAGTC 
TTGGAGCCAG AATATGAGCC TAATCCTCCA CTGGAAACTA CCACACAGCC TGACCCTGAA 
CCAGAGGGTT CAGTGCCAGT GCTAGCACCT GAGGCAGAGA TTCAACCACA CCCAGAGCTG 
GACCCGGAGA CTGAGACAGA GCCTGAGCCT GAGCCTGAGC CTAAGCCTGA GCCTGAGCCG 
GAACCTGAGC TTGAGCCTGA TCCCCAGTCT GGAGTAATAA TTGAGCCTCT GAGCAAGGCG 
GGAGAGGATA CAGTTAAGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_084457.2
CDS	68..1291
Translation	MMVFAFWKVFLILNCLAGQVSMVQVTIPDTFVNVTVGSNVTLLCLYTTTEKSLEKLSIQWSFFHNKEMEEPISIYYSEGGQASAIGQFKDRIIGATNPGNASITILHMQPADSGIYICDVNNPPDFVGKNQGLLDVTVLVKPSKPFCTIQGRPEAGHPISLSCLSAFGTPSPLYYWYKIEGNTIVPVKESFNTATGVLVIGNLTNFEQGYYQCTAINSLGNSSCEIDLTSSHPEVGIIIGALVGALIGAAVIICVVYFARNKVKSKQQKNLNSSTELEPMTKVHHPQQSEAISADGVQLEGTLPSSIHAGHNTEPTTTAVLEPEYEPNPPLETTTQPDPEPEGSVPVLAPEAEIQPHPELDPETETEPEPEPEPKPEPEPEPELEPDPQSGVIIEPLSKAGEDTVKA

Target ORF information:

RefSeq Version	NM_030181.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030181.3

ORF Insert Sequence:

ATGATGGTGT TTGCATTTTG GAAGGTCTTT CTGATCTTAA ACTGCCTTGC AGGTCAGGTC 
AGTATGGTGC AAGTGACCAT CCCAGACACT TTCGTGAATG TGACTGTTGG ATCTAATGTT 
ACTCTTCTCT GCCTCTATAC CACCACCGAG AAGTCCCTGG AAAAGCTTTC AATTCAATGG 
TCTTTCTTCC ACAATAAGGA AATGGAGGAG CCAATATCTA TCTACTACTC TGAAGGTGGA 
CAGGCTTCAG CTATTGGGCA ATTCAAAGAC CGAATAATTG GGGCTACTAA TCCCGGTAAT 
GCATCTATCA CCATATTGCA TATGCAGCCA GCAGACAGTG GAATTTACAT CTGTGATGTC 
AACAATCCTC CAGATTTTGT GGGCAAAAAC CAAGGCCTCC TTGACGTCAC TGTCTTAGTC 
AAACCTTCCA AGCCCTTTTG TACCATCCAA GGAAGACCAG AAGCAGGCCA TCCTATTTCC 
TTGTCTTGCC TTTCTGCCTT TGGAACACCG TCTCCTTTGT ATTACTGGTA TAAGATTGAG 
GGGAACACCA TTGTGCCAGT AAAAGAAAGT TTCAACACGG CCACTGGAGT TTTAGTTATT 
GGAAATCTAA CAAATTTTGA ACAAGGTTAT TACCAGTGTA CTGCCATCAA CAGTCTTGGC 
AATAGTTCCT GTGAAATTGA CTTAACGTCT TCACATCCAG AAGTTGGGAT CATCATCGGA 
GCTTTGGTCG GTGCCCTGAT AGGAGCTGCT GTTATCATCT GTGTGGTGTA CTTTGCCAGG 
AACAAAGTTA AATCCAAGCA GCAGAAGAAT TTAAATTCCA GCACAGAACT TGAGCCAATG 
ACAAAGGTAC ACCATCCCCA ACAAAGTGAG GCAATTTCAG CTGATGGCGT CCAGCTAGAA 
GGAACTCTGC CATCTTCCAT CCATGCTGGC CACAACACCG AACCTACTAC TACAGCAGTC 
TTGGAGCCAG AATATGAGCC TAATCCTCCA CTGGAAACTA CCACACAGCC TGACCCTGAA 
CCAGAGGGTT CAGTGCCAGT GCTAGCACCT GAGGCAGAGA TTCAACCACA CCCAGAGCTG 
GACCCGGAGA CTGAGACAGA GCCTGAGCCT GAGCCTGAGC CTAAGCCTGA GCCTGAGCCG 
GAACCTGAGC TTGAGCCTGA TCCCCAGTCT GGAGTAATAA TTGAGCCTCT GAGCAAGGCG 
GGAGAGGATA CAGTTAAGGC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vsig1 ( NM_001359143.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001346072.1 Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 3, mRNA.

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CloneID	OMu56555
Clone ID Related Accession (Same CDS sequence)	XM_006528630.3 , NM_001359143.1
Accession Version	NM_001359143.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Mus musculus(house mouse)
Product	V-set and immunoglobulin domain-containing protein 1 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132956.1 and BC145050.1. On Dec 22, 2017 this sequence version replaced XM_006528630.3. ##Evidence-Data-START## Transcript exon combination :: BC145050.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGTGT TTGCATTTTG GAAGGTCTTT CTGATCTTAA ACTGCCTTGC AGGTCAGGTC 
AGTATGGTGC AAGTGACCAT CCCAGACACT TTCGTGAATG TGACTGTTGG ATCTAATGTT 
ACTCTTCTCT GCCTCTATAC CACCACCGAG AAGTCCCTGG AAAAGCTTTC AATTCAATGG 
TCTTTCTTCC ACAATAAGGA AATGGAGGAG CCAATATCTC ATGTCTCGCA CCCCAAAACT 
GAGGGTATGG AGGAAAAGGC AGTCAGTCAG TGTCTAAAAA TGATGCATGC AAGAGACGCT 
CGGGGAAGAT GTAGCTGGAC CTCTCAGATC TACTACTCTG AAGGTGGACA GGCTTCAGCT 
ATTGGGCAAT TCAAAGACCG AATAATTGGG GCTACTAATC CCGGTAATGC ATCTATCACC 
ATATTGCATA TGCAGCCAGC AGACAGTGGA ATTTACATCT GTGATGTCAA CAATCCTCCA 
GATTTTGTGG GCAAAAACCA AGGCCTCCTT GACGTCACTG TCTTAGTCAA ACCTTCCAAG 
CCCTTTTGTA CCATCCAAGG AAGACCAGAA GCAGGCCATC CTATTTCCTT GTCTTGCCTT 
TCTGCCTTTG GAACACCGTC TCCTTTGTAT TACTGGTATA AGATTGAGGG GAACACCATT 
GTGCCAGTAA AAGAAAGTTT CAACACGGCC ACTGGAGTTT TAGTTATTGG AAATCTAACA 
AATTTTGAAC AAGGTTATTA CCAGTGTACT GCCATCAACA GTCTTGGCAA TAGTTCCTGT 
GAAATTGACT TAACGTCTTC ACATCCAGAA GTTGGGATCA TCATCGGAGC TTTGGTCGGT 
GCCCTGATAG GAGCTGCTGT TATCATCTGT GTGGTGTACT TTGCCAGGAA CAAAGTTAAA 
TCCAAGCAGC AGAAGAATTT AAATTCCAGC ACAGAACTTG AGCCAATGAC AAAGGTACAC 
CATCCCCAAC AAAGTGAGGC AATTTCAGCT GATGGCGTCC AGCTAGAAGG AACTCTGCCA 
TCTTCCATCC ATGCTGGCCA CAACACCGAA CCTACTACTA CAGCAGTCTT GGAGCCAGAA 
TATGAGCCTA ATCCTCCACT GGAAACTACC ACACAGCCTG ACCCTGAACC AGAGGGTTCA 
GTGCCAGTGC TAGCACCTGA GGCAGAGATT CAACCACACC CAGAGCTGGA CCCGGAGACT 
GAGACAGAGC CTGAGCCTGA GCCTGAGCCT AAGCCTGAGC CTGAGCCGGA ACCTGAGCTT 
GAGCCTGATC CCCAGTCTGG AGTAATAATT GAGCCTCTGA GCAAGGCGGG AGAGGATACA 
GTTAAGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001346072.1
CDS	68..1399
Translation	MMVFAFWKVFLILNCLAGQVSMVQVTIPDTFVNVTVGSNVTLLCLYTTTEKSLEKLSIQWSFFHNKEMEEPISHVSHPKTEGMEEKAVSQCLKMMHARDARGRCSWTSQIYYSEGGQASAIGQFKDRIIGATNPGNASITILHMQPADSGIYICDVNNPPDFVGKNQGLLDVTVLVKPSKPFCTIQGRPEAGHPISLSCLSAFGTPSPLYYWYKIEGNTIVPVKESFNTATGVLVIGNLTNFEQGYYQCTAINSLGNSSCEIDLTSSHPEVGIIIGALVGALIGAAVIICVVYFARNKVKSKQQKNLNSSTELEPMTKVHHPQQSEAISADGVQLEGTLPSSIHAGHNTEPTTTAVLEPEYEPNPPLETTTQPDPEPEGSVPVLAPEAEIQPHPELDPETETEPEPEPEPKPEPEPEPELEPDPQSGVIIEPLSKAGEDTVKA

Target ORF information:

RefSeq Version	NM_001359143.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001359143.1

ORF Insert Sequence:

ATGATGGTGT TTGCATTTTG GAAGGTCTTT CTGATCTTAA ACTGCCTTGC AGGTCAGGTC 
AGTATGGTGC AAGTGACCAT CCCAGACACT TTCGTGAATG TGACTGTTGG ATCTAATGTT 
ACTCTTCTCT GCCTCTATAC CACCACCGAG AAGTCCCTGG AAAAGCTTTC AATTCAATGG 
TCTTTCTTCC ACAATAAGGA AATGGAGGAG CCAATATCTC ATGTCTCGCA CCCCAAAACT 
GAGGGTATGG AGGAAAAGGC AGTCAGTCAG TGTCTAAAAA TGATGCATGC AAGAGACGCT 
CGGGGAAGAT GTAGCTGGAC CTCTCAGATC TACTACTCTG AAGGTGGACA GGCTTCAGCT 
ATTGGGCAAT TCAAAGACCG AATAATTGGG GCTACTAATC CCGGTAATGC ATCTATCACC 
ATATTGCATA TGCAGCCAGC AGACAGTGGA ATTTACATCT GTGATGTCAA CAATCCTCCA 
GATTTTGTGG GCAAAAACCA AGGCCTCCTT GACGTCACTG TCTTAGTCAA ACCTTCCAAG 
CCCTTTTGTA CCATCCAAGG AAGACCAGAA GCAGGCCATC CTATTTCCTT GTCTTGCCTT 
TCTGCCTTTG GAACACCGTC TCCTTTGTAT TACTGGTATA AGATTGAGGG GAACACCATT 
GTGCCAGTAA AAGAAAGTTT CAACACGGCC ACTGGAGTTT TAGTTATTGG AAATCTAACA 
AATTTTGAAC AAGGTTATTA CCAGTGTACT GCCATCAACA GTCTTGGCAA TAGTTCCTGT 
GAAATTGACT TAACGTCTTC ACATCCAGAA GTTGGGATCA TCATCGGAGC TTTGGTCGGT 
GCCCTGATAG GAGCTGCTGT TATCATCTGT GTGGTGTACT TTGCCAGGAA CAAAGTTAAA 
TCCAAGCAGC AGAAGAATTT AAATTCCAGC ACAGAACTTG AGCCAATGAC AAAGGTACAC 
CATCCCCAAC AAAGTGAGGC AATTTCAGCT GATGGCGTCC AGCTAGAAGG AACTCTGCCA 
TCTTCCATCC ATGCTGGCCA CAACACCGAA CCTACTACTA CAGCAGTCTT GGAGCCAGAA 
TATGAGCCTA ATCCTCCACT GGAAACTACC ACACAGCCTG ACCCTGAACC AGAGGGTTCA 
GTGCCAGTGC TAGCACCTGA GGCAGAGATT CAACCACACC CAGAGCTGGA CCCGGAGACT 
GAGACAGAGC CTGAGCCTGA GCCTGAGCCT AAGCCTGAGC CTGAGCCGGA ACCTGAGCTT 
GAGCCTGATC CCCAGTCTGG AGTAATAATT GAGCCTCTGA GCAAGGCGGG AGAGGATACA 
GTTAAGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vsig1 ( XM_006528630.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528693.1 Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56555
Clone ID Related Accession (Same CDS sequence)	XM_006528630.3 , NM_001359143.1
Accession Version	XM_006528630.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	V-set and immunoglobulin domain-containing protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528630.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGTGT TTGCATTTTG GAAGGTCTTT CTGATCTTAA ACTGCCTTGC AGGTCAGGTC 
AGTATGGTGC AAGTGACCAT CCCAGACACT TTCGTGAATG TGACTGTTGG ATCTAATGTT 
ACTCTTCTCT GCCTCTATAC CACCACCGAG AAGTCCCTGG AAAAGCTTTC AATTCAATGG 
TCTTTCTTCC ACAATAAGGA AATGGAGGAG CCAATATCTC ATGTCTCGCA CCCCAAAACT 
GAGGGTATGG AGGAAAAGGC AGTCAGTCAG TGTCTAAAAA TGATGCATGC AAGAGACGCT 
CGGGGAAGAT GTAGCTGGAC CTCTCAGATC TACTACTCTG AAGGTGGACA GGCTTCAGCT 
ATTGGGCAAT TCAAAGACCG AATAATTGGG GCTACTAATC CCGGTAATGC ATCTATCACC 
ATATTGCATA TGCAGCCAGC AGACAGTGGA ATTTACATCT GTGATGTCAA CAATCCTCCA 
GATTTTGTGG GCAAAAACCA AGGCCTCCTT GACGTCACTG TCTTAGTCAA ACCTTCCAAG 
CCCTTTTGTA CCATCCAAGG AAGACCAGAA GCAGGCCATC CTATTTCCTT GTCTTGCCTT 
TCTGCCTTTG GAACACCGTC TCCTTTGTAT TACTGGTATA AGATTGAGGG GAACACCATT 
GTGCCAGTAA AAGAAAGTTT CAACACGGCC ACTGGAGTTT TAGTTATTGG AAATCTAACA 
AATTTTGAAC AAGGTTATTA CCAGTGTACT GCCATCAACA GTCTTGGCAA TAGTTCCTGT 
GAAATTGACT TAACGTCTTC ACATCCAGAA GTTGGGATCA TCATCGGAGC TTTGGTCGGT 
GCCCTGATAG GAGCTGCTGT TATCATCTGT GTGGTGTACT TTGCCAGGAA CAAAGTTAAA 
TCCAAGCAGC AGAAGAATTT AAATTCCAGC ACAGAACTTG AGCCAATGAC AAAGGTACAC 
CATCCCCAAC AAAGTGAGGC AATTTCAGCT GATGGCGTCC AGCTAGAAGG AACTCTGCCA 
TCTTCCATCC ATGCTGGCCA CAACACCGAA CCTACTACTA CAGCAGTCTT GGAGCCAGAA 
TATGAGCCTA ATCCTCCACT GGAAACTACC ACACAGCCTG ACCCTGAACC AGAGGGTTCA 
GTGCCAGTGC TAGCACCTGA GGCAGAGATT CAACCACACC CAGAGCTGGA CCCGGAGACT 
GAGACAGAGC CTGAGCCTGA GCCTGAGCCT AAGCCTGAGC CTGAGCCGGA ACCTGAGCTT 
GAGCCTGATC CCCAGTCTGG AGTAATAATT GAGCCTCTGA GCAAGGCGGG AGAGGATACA 
GTTAAGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528693.1
CDS	161..1492
Translation	MMVFAFWKVFLILNCLAGQVSMVQVTIPDTFVNVTVGSNVTLLCLYTTTEKSLEKLSIQWSFFHNKEMEEPISHVSHPKTEGMEEKAVSQCLKMMHARDARGRCSWTSQIYYSEGGQASAIGQFKDRIIGATNPGNASITILHMQPADSGIYICDVNNPPDFVGKNQGLLDVTVLVKPSKPFCTIQGRPEAGHPISLSCLSAFGTPSPLYYWYKIEGNTIVPVKESFNTATGVLVIGNLTNFEQGYYQCTAINSLGNSSCEIDLTSSHPEVGIIIGALVGALIGAAVIICVVYFARNKVKSKQQKNLNSSTELEPMTKVHHPQQSEAISADGVQLEGTLPSSIHAGHNTEPTTTAVLEPEYEPNPPLETTTQPDPEPEGSVPVLAPEAEIQPHPELDPETETEPEPEPEPKPEPEPEPELEPDPQSGVIIEPLSKAGEDTVKA

Target ORF information:

RefSeq Version	XM_006528630.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus V-set and immunoglobulin domain containing 1 (Vsig1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528630.3

ORF Insert Sequence:

ATGATGGTGT TTGCATTTTG GAAGGTCTTT CTGATCTTAA ACTGCCTTGC AGGTCAGGTC 
AGTATGGTGC AAGTGACCAT CCCAGACACT TTCGTGAATG TGACTGTTGG ATCTAATGTT 
ACTCTTCTCT GCCTCTATAC CACCACCGAG AAGTCCCTGG AAAAGCTTTC AATTCAATGG 
TCTTTCTTCC ACAATAAGGA AATGGAGGAG CCAATATCTC ATGTCTCGCA CCCCAAAACT 
GAGGGTATGG AGGAAAAGGC AGTCAGTCAG TGTCTAAAAA TGATGCATGC AAGAGACGCT 
CGGGGAAGAT GTAGCTGGAC CTCTCAGATC TACTACTCTG AAGGTGGACA GGCTTCAGCT 
ATTGGGCAAT TCAAAGACCG AATAATTGGG GCTACTAATC CCGGTAATGC ATCTATCACC 
ATATTGCATA TGCAGCCAGC AGACAGTGGA ATTTACATCT GTGATGTCAA CAATCCTCCA 
GATTTTGTGG GCAAAAACCA AGGCCTCCTT GACGTCACTG TCTTAGTCAA ACCTTCCAAG 
CCCTTTTGTA CCATCCAAGG AAGACCAGAA GCAGGCCATC CTATTTCCTT GTCTTGCCTT 
TCTGCCTTTG GAACACCGTC TCCTTTGTAT TACTGGTATA AGATTGAGGG GAACACCATT 
GTGCCAGTAA AAGAAAGTTT CAACACGGCC ACTGGAGTTT TAGTTATTGG AAATCTAACA 
AATTTTGAAC AAGGTTATTA CCAGTGTACT GCCATCAACA GTCTTGGCAA TAGTTCCTGT 
GAAATTGACT TAACGTCTTC ACATCCAGAA GTTGGGATCA TCATCGGAGC TTTGGTCGGT 
GCCCTGATAG GAGCTGCTGT TATCATCTGT GTGGTGTACT TTGCCAGGAA CAAAGTTAAA 
TCCAAGCAGC AGAAGAATTT AAATTCCAGC ACAGAACTTG AGCCAATGAC AAAGGTACAC 
CATCCCCAAC AAAGTGAGGC AATTTCAGCT GATGGCGTCC AGCTAGAAGG AACTCTGCCA 
TCTTCCATCC ATGCTGGCCA CAACACCGAA CCTACTACTA CAGCAGTCTT GGAGCCAGAA 
TATGAGCCTA ATCCTCCACT GGAAACTACC ACACAGCCTG ACCCTGAACC AGAGGGTTCA 
GTGCCAGTGC TAGCACCTGA GGCAGAGATT CAACCACACC CAGAGCTGGA CCCGGAGACT 
GAGACAGAGC CTGAGCCTGA GCCTGAGCCT AAGCCTGAGC CTGAGCCGGA ACCTGAGCTT 
GAGCCTGATC CCCAGTCTGG AGTAATAATT GAGCCTCTGA GCAAGGCGGG AGAGGATACA 
GTTAAGGCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The NANCI-Nkx2.1 gene duplex buffers Nkx2.1 expression to maintain lung development and homeostasis.
Genes & development31(9)889-903(2017 May)
Herriges MJ,Tischfield DJ,Cui Z,Morley MP,Han Y,Babu A,Li S,Lu M,Cendan I,Garcia BA,Anderson SA,Morrisey EE

Female-to-male sex reversal in mice caused by transgenic overexpression of Dmrt1.
Development (Cambridge, England)142(6)1083-8(2015 Mar)
Zhao L,Svingen T,Ng ET,Koopman P

Impairments to the GH-IGF-I axis in hSOD1G93A mice give insight into possible mechanisms of GH dysregulation in patients with amyotrophic lateral sclerosis.
Endocrinology153(8)3735-46(2012 Aug)
Steyn FJ,Ngo ST,Lee JD,Leong JW,Buckley AJ,Veldhuis JD,McCombe PA,Chen C,Bellingham MC

Adhesion protein VSIG1 is required for the proper differentiation of glandular gastric epithelia.
PloS one6(10)e25908(2011)
Oidovsambuu O,Nyamsuren G,Liu S,G?ring W,Engel W,Adham IM

Extracellular domain of V-set and immunoglobulin domain containing 1 (VSIG1) interacts with sertoli cell membrane protein, while its PDZ-binding motif forms a complex with ZO-1.
Molecules and cells30(5)443-8(2010 Nov)
Kim E,Lee Y,Kim JS,Song BS,Kim SU,Huh JW,Lee SR,Kim SH,Hong Y,Chang KT

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Vsig1 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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