Spag11a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spag11a
Entrez Gene ID	78128
Full Name	sperm associated antigen 11A
Synonyms	9230111C08Rik,Bin1b,EP2Q,EP2e,Spag11
General protein information	Preferred Names sperm associated antigen 11A Names sperm-associated antigen 11 antimicrobial peptide Bin1b beta defensin EP2e
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 A1.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_153115.1	NP_694755.1	sperm-associated antigen 11 precursor
XM_006508927.3	XP_006508990.1	sperm-associated antigen 11 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPAG11E	NP_001165016.1
Bos taurus (cattle)	LOC101905775	XP_005225990.1
Rattus norvegicus (Norway rat)	Spag11b	NP_659555.1
Mus musculus (house mouse)	Spag11a	NP_694755.1

Gene Ontology
Bibliography

Related articles in PubMed

Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.
Pujianto DA, Loanda E, Sari P, Midoen YH, Soeharso P
Reproductive biology and endocrinology : RB&E1159(2013 Jul)

mBin1b transgenic mice show enhanced resistance to epididymal infection by bacteria challenge.
Fei Z, Hu S, Xiao L, Zhou J, Diao H, Yu H, Fang S, Wang Y, Wan Y, Wang W, He Y, Wang C, Xu G, Wang Z, Zhang Y, Fei J
Genes and immunity13(6)445-51(2012 Sep)

Antimicrobial responses in the male reproductive tract of lipopolysaccharide challenged rats.
Biswas B, Yenugu S
American journal of reproductive immunology (New York, N.Y. : 1989)65(6)557-68(2011 Jun)

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
Amid C, Rehaume LM, Brown KL, Gilbert JG, Dougan G, Hancock RE, Harrow JL
BMC genomics10606(2009 Dec)

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Patil AA, Cai Y, Sang Y, Blecha F, Zhang G
Physiological genomics23(1)5-17(2005 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Spag11a has a specific role in epididymal sperm maturation.
Title: Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.

Model was developed in which Bin1b was exclusively overexpressed by up to 50-fold in the caput epididymis. The transgenic mice are healthy with normal reproductive activity, but are more resistant to epididymal infection from E coli than normal animals.
Title: mBin1b transgenic mice show enhanced resistance to epididymal infection by bacteria challenge.

increased expression in response to lipopolysaccharide administration
Title: Antimicrobial responses in the male reproductive tract of lipopolysaccharide challenged rats.

The epididymis-specific expression pattern of the beta-defensin isoform EP2e has been characterized.
Title: Identification of multiple novel epididymis-specific beta-defensin isoforms in humans and mice.

The following Spag11a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spag11a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02344	NM_153115.1 Latest version!	Mus musculus sperm associated antigen 11A (Spag11a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu02344	XM_006508927.3 Latest version!	Mus musculus sperm associated antigen 11A (Spag11a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spag11a ( NM_153115.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_694755.1 Mus musculus sperm associated antigen 11A (Spag11a), mRNA.

Price & Availability↑

CloneID	OMu02344
Clone ID Related Accession (Same CDS sequence)	NM_153115.1 , XM_006508927.3
Accession Version	NM_153115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 11 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AB089183.1. ##Evidence-Data-START## Transcript exon combination :: AB089183.1, AK020333.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGAAAGTCT TGTTACTCTT TGCTGTCTTC TTCTGCTTTG TCCAAGGAAA CTCAGGGGAC 
ATCCCACCCG GAATCAGAAA CACCGTGTGC CTCATGCAAC AGGGCCACTG CAGGCTCTTC 
ATGTGCCGTT CTGGTGAGAG AAAAGGGGAT ATCTGCTCCG ACCCCTGGAA CAGATGCTGC 
GTCCCCTATT CTGTTAAAGA CAGAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_694755.1
CDS	59..268
Translation	MKVLLLFAVFFCFVQGNSGDIPPGIRNTVCLMQQGHCRLFMCRSGERKGDICSDPWNRCCVPYSVKDRR

Target ORF information:

RefSeq Version	NM_153115.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 11A (Spag11a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153115.1

ORF Insert Sequence:

ATGAAAGTCT TGTTACTCTT TGCTGTCTTC TTCTGCTTTG TCCAAGGAAA CTCAGGGGAC 
ATCCCACCCG GAATCAGAAA CACCGTGTGC CTCATGCAAC AGGGCCACTG CAGGCTCTTC 
ATGTGCCGTT CTGGTGAGAG AAAAGGGGAT ATCTGCTCCG ACCCCTGGAA CAGATGCTGC 
GTCCCCTATT CTGTTAAAGA CAGAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spag11a ( XM_006508927.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006508990.1 Mus musculus sperm associated antigen 11A (Spag11a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu02344
Clone ID Related Accession (Same CDS sequence)	NM_153115.1 , XM_006508927.3
Accession Version	XM_006508927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	sperm-associated antigen 11 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006508927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTCT TGTTACTCTT TGCTGTCTTC TTCTGCTTTG TCCAAGGAAA CTCAGGGGAC 
ATCCCACCCG GAATCAGAAA CACCGTGTGC CTCATGCAAC AGGGCCACTG CAGGCTCTTC 
ATGTGCCGTT CTGGTGAGAG AAAAGGGGAT ATCTGCTCCG ACCCCTGGAA CAGATGCTGC 
GTCCCCTATT CTGTTAAAGA CAGAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006508990.1
CDS	733..942
Translation	MKVLLLFAVFFCFVQGNSGDIPPGIRNTVCLMQQGHCRLFMCRSGERKGDICSDPWNRCCVPYSVKDRR

Target ORF information:

RefSeq Version	XM_006508927.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus sperm associated antigen 11A (Spag11a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006508927.3

ORF Insert Sequence:

ATGAAAGTCT TGTTACTCTT TGCTGTCTTC TTCTGCTTTG TCCAAGGAAA CTCAGGGGAC 
ATCCCACCCG GAATCAGAAA CACCGTGTGC CTCATGCAAC AGGGCCACTG CAGGCTCTTC 
ATGTGCCGTT CTGGTGAGAG AAAAGGGGAT ATCTGCTCCG ACCCCTGGAA CAGATGCTGC 
GTCCCCTATT CTGTTAAAGA CAGAAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sperm-associated antigen 11A is expressed exclusively in the principal cells of the mouse caput epididymis in an androgen-dependent manner.
Reproductive biology and endocrinology : RB&E1159(2013 Jul)
Pujianto DA,Loanda E,Sari P,Midoen YH,Soeharso P

mBin1b transgenic mice show enhanced resistance to epididymal infection by bacteria challenge.
Genes and immunity13(6)445-51(2012 Sep)
Fei Z,Hu S,Xiao L,Zhou J,Diao H,Yu H,Fang S,Wang Y,Wan Y,Wang W,He Y,Wang C,Xu G,Wang Z,Zhang Y,Fei J

Antimicrobial responses in the male reproductive tract of lipopolysaccharide challenged rats.
American journal of reproductive immunology (New York, N.Y. : 1989)65(6)557-68(2011 Jun)
Biswas B,Yenugu S

Manual annotation and analysis of the defensin gene cluster in the C57BL/6J mouse reference genome.
BMC genomics10606(2009 Dec)
Amid C,Rehaume LM,Brown KL,Gilbert JG,Dougan G,Hancock RE,Harrow JL

Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract.
Physiological genomics23(1)5-17(2005 Sep)
Patil AA,Cai Y,Sang Y,Blecha F,Zhang G