Rflnb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rflnb
Entrez Gene ID	76566
Full Name	refilin B
Synonyms	1500005K14Rik,Fam101b,RefilinB,cfm
General protein information	Preferred Names refilin B Names refilin-B family with sequence similarity 101, member B filamin-interacting protein FAM101B protein FAM101B regulator of filamin protein B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B5

mRNA and Protein(s)

mRNA	Protein	Name
NM_029658.1	NP_083934.1	refilin-B

Pathways from BioSystems

Homologies

Homo sapiens (human)	FAM101B	NP_874364.1
	LOC699332	XP_001083814.2
Canis lupus familiaris (dog)	FAM101B	XP_853944.1
Mus musculus (house mouse)	Fam101b	NP_083934.1
Rattus norvegicus (Norway rat)	Fam101b	NP_001007612.1
Gallus gallus (chicken)	FAM101B	XP_415867.3
Bos taurus (cattle)	FAM101B	XP_002695757.2
Pan troglodytes (chimpanzee)	FAM101B	XP_511242.3
Danio rerio (zebrafish)	fam101b	XP_002664671.1
Xenopus tropicalis (tropical clawed frog)	fam101b	NP_001008059.1

Gene Ontology
Bibliography

Related articles in PubMed

Filamin-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeletal elements.
Mizuhashi K, Kanamoto T, Moriishi T, Muranishi Y, Miyazaki T, Terada K, Omori Y, Ito M, Komori T, Furukawa T
Human molecular genetics23(11)2953-67(2014 Jun)

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Shalom-Barak T, Zhang X, Chu T, Timothy Schaiff W, Reddy JK, Xu J, Sadovsky Y, Barak Y
Developmental biology372(1)143-55(2012 Dec)

RefilinB (FAM101B) targets filamin A to organize perinuclear actin networks and regulates nuclear shape.
Gay O, Gilquin B, Nakamura F, Jenkins ZA, McCartney R, Krakow D, Deshiere A, Assard N, Hartwig JH, Robertson SP, Baudier J
Proceedings of the National Academy of Sciences of the United States of America108(28)11464-9(2011 Jul)

Abnormal development of the locus coeruleus in Ear2(Nr2f6)-deficient mice impairs the functionality of the forebrain clock and affects nociception.
Warnecke M, Oster H, Revelli JP, Alvarez-Bolado G, Eichele G
Genes & development19(5)614-25(2005 Mar)

cfm is a novel gene uniquely expressed in developing forebrain and midbrain, but its null mutant exhibits no obvious phenotype.
Hirano M, Murata T, Furushima K, Kiyonari H, Nakamura M, Suda Y, Aizawa S
Gene expression patterns : GEP5(3)439-44(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Filamin a, b-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeleton.
Title: Filamin-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeletal elements.

The following Rflnb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rflnb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu19315	NM_029658.1 Latest version!	Mus musculus refilin B (Rflnb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rflnb ( NM_029658.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083934.1 Mus musculus refilin B (Rflnb), mRNA.

Price & Availability↑

CloneID	OMu19315
Clone ID Related Accession (Same CDS sequence)	NM_029658.1
Accession Version	NM_029658.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	refilin-B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK005164.1, AA016881.1, AL645569.21 and W81812.1. On or before Nov 18, 2009 this sequence version replaced XM_983541.2, XM_893392.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK005164.1, SRR6238556.2882095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849376 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGGCC GGCTGAGCCT GCAGGATGTC CCTGAACTCG TGGACACGAA GAAAAAAGGC 
GACGGCGTCC TGGACAGCCC GGACTCGGGG CTGCCCCCAA GCCCTAGCCC CAGCCACTGG 
GGGCTCGCAG CGACGGCGGG CGGCGGTGGG GAGCGCGCTC CGGTCGCAGG GACGCTGGAG 
CCCGATGCGG CGGTGACCCC TATAGTCCCG AACCCAGCAT CTCTGACCCA CTCGCTAGCT 
GCCATCTGCT CACCGAGACT CTGTCCCCTG TCCTTCGGCG AAGGAGTTGA GTTTGACCCC 
TTGCCACCAA AGGAAATAAA GTATACCTCC TCAGTCAAGT ATGACTCTGA GCGGCACTTC 
ATCGATGATG TACAGATGCC CCTGGGTCTG GTGGTGGCTT CCTGCAGCCA GACAGTCACC 
TGTATCCCCA ACTGCACTTG GCGAAACTAT AAGGCGGAAG TGCGCTTCGA GCCACGCCCC 
AAGCCCGCGC GTTTCCTCAG CACCACCATC GTCTACCCCA AGTACCCCAA AACCGTCTAC 
ACCACCACTC TGGATTACAA CTGCCACAAG AAGCTGAGGA GGTTTCTGTC CAGTGTGGAG 
CTCGAGGCCA CGGAGTTCCT GGGCAGCGAT GGGCTAGCCG ATGAATGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083934.1
CDS	146..796
Translation	MVGRLSLQDVPELVDTKKKGDGVLDSPDSGLPPSPSPSHWGLAATAGGGGERAPVAGTLEPDAAVTPIVPNPASLTHSLAAICSPRLCPLSFGEGVEFDPLPPKEIKYTSSVKYDSERHFIDDVQMPLGLVVASCSQTVTCIPNCTWRNYKAEVRFEPRPKPARFLSTTIVYPKYPKTVYTTTLDYNCHKKLRRFLSSVELEATEFLGSDGLADEC

Target ORF information:

RefSeq Version	NM_029658.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus refilin B (Rflnb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029658.1

ORF Insert Sequence:

ATGGTGGGCC GGCTGAGCCT GCAGGATGTC CCTGAACTCG TGGACACGAA GAAAAAAGGC 
GACGGCGTCC TGGACAGCCC GGACTCGGGG CTGCCCCCAA GCCCTAGCCC CAGCCACTGG 
GGGCTCGCAG CGACGGCGGG CGGCGGTGGG GAGCGCGCTC CGGTCGCAGG GACGCTGGAG 
CCCGATGCGG CGGTGACCCC TATAGTCCCG AACCCAGCAT CTCTGACCCA CTCGCTAGCT 
GCCATCTGCT CACCGAGACT CTGTCCCCTG TCCTTCGGCG AAGGAGTTGA GTTTGACCCC 
TTGCCACCAA AGGAAATAAA GTATACCTCC TCAGTCAAGT ATGACTCTGA GCGGCACTTC 
ATCGATGATG TACAGATGCC CCTGGGTCTG GTGGTGGCTT CCTGCAGCCA GACAGTCACC 
TGTATCCCCA ACTGCACTTG GCGAAACTAT AAGGCGGAAG TGCGCTTCGA GCCACGCCCC 
AAGCCCGCGC GTTTCCTCAG CACCACCATC GTCTACCCCA AGTACCCCAA AACCGTCTAC 
ACCACCACTC TGGATTACAA CTGCCACAAG AAGCTGAGGA GGTTTCTGTC CAGTGTGGAG 
CTCGAGGCCA CGGAGTTCCT GGGCAGCGAT GGGCTAGCCG ATGAATGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Filamin-interacting proteins, Cfm1 and Cfm2, are essential for the formation of cartilaginous skeletal elements.
Human molecular genetics23(11)2953-67(2014 Jun)
Mizuhashi K,Kanamoto T,Moriishi T,Muranishi Y,Miyazaki T,Terada K,Omori Y,Ito M,Komori T,Furukawa T

Placental PPAR? regulates spatiotemporally diverse genes and a unique metabolic network.
Developmental biology372(1)143-55(2012 Dec)
Shalom-Barak T,Zhang X,Chu T,Timothy Schaiff W,Reddy JK,Xu J,Sadovsky Y,Barak Y

RefilinB (FAM101B) targets filamin A to organize perinuclear actin networks and regulates nuclear shape.
Proceedings of the National Academy of Sciences of the United States of America108(28)11464-9(2011 Jul)
Gay O,Gilquin B,Nakamura F,Jenkins ZA,McCartney R,Krakow D,Deshiere A,Assard N,Hartwig JH,Robertson SP,Baudier J

Abnormal development of the locus coeruleus in Ear2(Nr2f6)-deficient mice impairs the functionality of the forebrain clock and affects nociception.
Genes & development19(5)614-25(2005 Mar)
Warnecke M,Oster H,Revelli JP,Alvarez-Bolado G,Eichele G

cfm is a novel gene uniquely expressed in developing forebrain and midbrain, but its null mutant exhibits no obvious phenotype.
Gene expression patterns : GEP5(3)439-44(2005 Feb)
Hirano M,Murata T,Furushima K,Kiyonari H,Nakamura M,Suda Y,Aizawa S