Ndfip2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ndfip2
Entrez Gene ID	76273
Full Name	Nedd4 family interacting protein 2
Synonyms	0710001O20Rik,2810436B12Rik,9130207N19Rik,N4wbp5a,mKIAA1165
General protein information	Preferred Names Nedd4 family interacting protein 2 Names NEDD4 family-interacting protein 2 NEDD4 WW domain-binding protein 5A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 E2.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190989.1	NP_001177918.1	NEDD4 family-interacting protein 2
NM_029561.4	NP_083837.2	NEDD4 family-interacting protein 2
NM_029561.5	NP_083837.2	NEDD4 family-interacting protein 2
NM_001190989.2	NP_001177918.1	NEDD4 family-interacting protein 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ndfip2	NP_083837.2
Xenopus tropicalis (tropical clawed frog)	ndfip2	NP_001016994.1
	NDFIP2	XP_001092556.2
Bos taurus (cattle)	NDFIP2	NP_001192649.1
Rattus norvegicus (Norway rat)	Ndfip2	NP_001101860.2
Pan troglodytes (chimpanzee)	NDFIP2	XP_522688.3
Gallus gallus (chicken)	NDFIP2	NP_001025991.1
Canis lupus familiaris (dog)	NDFIP2	XP_534156.2
Danio rerio (zebrafish)	ndfip2	NP_998512.1
Homo sapiens (human)	NDFIP2	NP_061953.2

Gene Ontology
Bibliography

Related articles in PubMed

NDFIP allows NEDD4/NEDD4L-induced AQP2 ubiquitination and degradation.
Trimpert C, Wesche D, de Groot T, Pimentel Rodriguez MM, Wong V, van den Berg DTM, Cheval L, Ariza CA, Doucet A, Stagljar I, Deen PMT
PloS one12(9)e0183774(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells.
O'Leary CE, Riling CR, Spruce LA, Ding H, Kumar S, Deng G, Liu Y, Seeholzer SH, Oliver PM
Nature communications711226(2016 Apr)

Ndfip2 is a potential regulator of the iron transporter DMT1 in the liver.
Foot NJ, Gembus KM, Mackenzie K, Kumar S
Scientific reports624045(2016 Apr)

Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2.
Mund T, Pelham HR
Proceedings of the National Academy of Sciences of the United States of America107(25)11429-34(2010 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate thar Janus kinase 1 (Jak1) degradation is dependent on Nedd4 family interacting proteins Ndfip1/Ndfip2.
Title: Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells.

Ndfip2 controls DMT1 in the liver with female mice showing a greater response to altered dietary iron than the male mice
Title: Ndfip2 is a potential regulator of the iron transporter DMT1 in the liver.

DMT1 interacts with 2 WW domain-interacting proteins, Ndfip1 and Ndfip2. This promotes DMT1 ubiquitination and degradation by WWP2.
Title: Regulation of the divalent metal ion transporter DMT1 and iron homeostasis by a ubiquitin-dependent mechanism involving Ndfips and WWP2.

The following Ndfip2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ndfip2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu01401	NM_029561.5 Latest version!	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu01401	NM_001190989.2 Latest version!	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OMu01401	NM_029561.4	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu01401	NM_001190989.1	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ndfip2 ( NM_001190989.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001177918.1 Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01401
Clone ID Related Accession (Same CDS sequence)	NM_001190989.1 , NM_029561.4 , NM_029561.5 , NM_001190989.2
Accession Version	NM_001190989.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-03
Organism	Mus musculus(house mouse)
Product	NEDD4 family-interacting protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113125.8, BY159111.1, AK132235.1, CX206555.1 and AC141878.4. Transcript Variant: This variant (2) differs in the 3' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.271664.1, BC106125.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177918.1
CDS	1..1017
Translation	MGRRRSQRACASAPSMLSSARGSPELRRRSASDAELSAGAEGATGSEAAPPGDLGGRTRGGGRGSAAAAATTSTREAEGAERRGDTPARKPDPEAGRMDHHQLGTGRYQVLHNEEDNSESSAVEQPSTSSLAAPTVEAAASAPALDPDSPPPYSSITVEAPTTSDTDVYSEFYPVPPPYSVATSLPTYDEAEKAKAAALAAAAADAPQRNQEEDCTPRDDFSDVEQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRYFFLL

Target ORF information:

RefSeq Version	NM_001190989.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190989.1

ORF Insert Sequence:

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndfip2 ( NM_029561.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083837.2 Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01401
Clone ID Related Accession (Same CDS sequence)	NM_001190989.1 , NM_029561.4 , NM_029561.5 , NM_001190989.2
Accession Version	NM_029561.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-03
Organism	Mus musculus(house mouse)
Product	NEDD4 family-interacting protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113125.8, BY159111.1, AK132235.1 and AC141878.4. On Jul 9, 2010 this sequence version replaced NM_029561.3. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments and orthologous data. ##Evidence-Data-START## Transcript exon combination :: BC071237.1, AK132235.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083837.2
CDS	1..1017
Translation	MGRRRSQRACASAPSMLSSARGSPELRRRSASDAELSAGAEGATGSEAAPPGDLGGRTRGGGRGSAAAAATTSTREAEGAERRGDTPARKPDPEAGRMDHHQLGTGRYQVLHNEEDNSESSAVEQPSTSSLAAPTVEAAASAPALDPDSPPPYSSITVEAPTTSDTDVYSEFYPVPPPYSVATSLPTYDEAEKAKAAALAAAAADAPQRNQEEDCTPRDDFSDVEQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRYFFLL

Target ORF information:

RefSeq Version	NM_029561.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029561.4

ORF Insert Sequence:

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndfip2 ( NM_029561.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083837.2 Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu01401
Clone ID Related Accession (Same CDS sequence)	NM_001190989.1 , NM_029561.4 , NM_029561.5 , NM_001190989.2
Accession Version	NM_029561.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Mus musculus(house mouse)
Product	NEDD4 family-interacting protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113125.8 and AC141878.4. On Feb 16, 2019 this sequence version replaced NM_029561.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1001647.1, SRR7345562.2304772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083837.2
CDS	51..1067
Translation	MGRRRSQRACASAPSMLSSARGSPELRRRSASDAELSAGAEGATGSEAAPPGDLGGRTRGGGRGSAAAAATTSTREAEGAERRGDTPARKPDPEAGRMDHHQLGTGRYQVLHNEEDNSESSAVEQPSTSSLAAPTVEAAASAPALDPDSPPPYSSITVEAPTTSDTDVYSEFYPVPPPYSVATSLPTYDEAEKAKAAALAAAAADAPQRNQEEDCTPRDDFSDVEQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRYFFLL

Target ORF information:

RefSeq Version	NM_029561.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029561.5

ORF Insert Sequence:

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ndfip2 ( NM_001190989.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001177918.1 Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu01401
Clone ID Related Accession (Same CDS sequence)	NM_001190989.1 , NM_029561.4 , NM_029561.5 , NM_001190989.2
Accession Version	NM_001190989.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Mus musculus(house mouse)
Product	NEDD4 family-interacting protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC113125.8 and AC141878.4. On Feb 16, 2019 this sequence version replaced NM_001190989.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2187148.1, SRR7345562.162630.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177918.1
CDS	51..1067
Translation	MGRRRSQRACASAPSMLSSARGSPELRRRSASDAELSAGAEGATGSEAAPPGDLGGRTRGGGRGSAAAAATTSTREAEGAERRGDTPARKPDPEAGRMDHHQLGTGRYQVLHNEEDNSESSAVEQPSTSSLAAPTVEAAASAPALDPDSPPPYSSITVEAPTTSDTDVYSEFYPVPPPYSVATSLPTYDEAEKAKAAALAAAAADAPQRNQEEDCTPRDDFSDVEQLRVGNDGIFMLAFFMAFIFNWLGFCLSFCITNTIAGRYGAICGFGLSLIKWILIVRFSDYFTGYFNGQYWLWWIFLVLGLLLFFRGFVNYLKVRNMSESMAAAHRTRYFFLL

Target ORF information:

RefSeq Version	NM_001190989.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Nedd4 family interacting protein 2 (Ndfip2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190989.2

ORF Insert Sequence:

ATGGGACGCC GGCGGAGCCA GAGAGCATGC GCGTCCGCGC CGAGCATGCT CAGTAGCGCG 
CGCGGCTCCC CGGAGCTCCG GCGCCGATCG GCGAGCGACG CGGAGCTCTC CGCTGGAGCC 
GAGGGAGCGA CAGGAAGTGA AGCGGCCCCG CCAGGCGACC TCGGCGGCAG AACCCGAGGC 
GGCGGAAGAG GCTCTGCGGC GGCGGCGGCG ACGACGTCGA CCCGGGAGGC CGAGGGAGCT 
GAGCGCCGAG GGGACACGCC CGCTCGGAAG CCGGACCCCG AGGCGGGCAG GATGGATCAC 
CACCAGCTGG GGACTGGTCG CTACCAGGTG CTTCACAATG AAGAAGATAA CTCAGAATCA 
TCTGCTGTGG AGCAGCCGTC AACTTCTAGC TTAGCAGCGC CGACTGTGGA GGCTGCCGCT 
TCAGCACCAG CACTGGACCC TGACTCTCCT CCTCCTTACA GCAGCATCAC TGTGGAAGCT 
CCTACCACTT CAGATACTGA CGTGTATAGT GAGTTTTACC CTGTGCCACC TCCGTACAGT 
GTTGCTACCT CTCTGCCTAC ATATGACGAA GCTGAAAAGG CTAAAGCTGC TGCCCTGGCA 
GCTGCTGCAG CAGATGCACC TCAGAGAAAC CAAGAGGAAG ACTGTACGCC ACGGGATGAC 
TTCAGTGATG TAGAGCAGCT CAGAGTGGGC AACGACGGCA TTTTCATGCT GGCCTTTTTC 
ATGGCATTCA TATTCAACTG GCTTGGGTTC TGCTTATCCT TCTGTATCAC CAATACCATC 
GCTGGAAGAT ACGGTGCTAT CTGTGGGTTT GGCCTTTCAC TGATCAAATG GATCCTTATT 
GTCAGGTTTT CTGACTATTT TACTGGCTAT TTCAATGGAC AGTATTGGCT TTGGTGGATA 
TTTCTCGTAC TTGGCCTGCT CCTTTTCTTC AGAGGGTTTG TTAATTACCT AAAAGTCAGA 
AACATGTCTG AAAGTATGGC GGCTGCACAT AGGACGAGGT ACTTCTTCCT GCTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NDFIP allows NEDD4/NEDD4L-induced AQP2 ubiquitination and degradation.
PloS one12(9)e0183774(2017)
Trimpert C,Wesche D,de Groot T,Pimentel Rodriguez MM,Wong V,van den Berg DTM,Cheval L,Ariza CA,Doucet A,Stagljar I,Deen PMT

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Ndfip-mediated degradation of Jak1 tunes cytokine signalling to limit expansion of CD4+ effector T cells.
Nature communications711226(2016 Apr)
O'Leary CE,Riling CR,Spruce LA,Ding H,Kumar S,Deng G,Liu Y,Seeholzer SH,Oliver PM

Ndfip2 is a potential regulator of the iron transporter DMT1 in the liver.
Scientific reports624045(2016 Apr)
Foot NJ,Gembus KM,Mackenzie K,Kumar S

Regulation of PTEN/Akt and MAP kinase signaling pathways by the ubiquitin ligase activators Ndfip1 and Ndfip2.
Proceedings of the National Academy of Sciences of the United States of America107(25)11429-34(2010 Jun)
Mund T,Pelham HR

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Ndfip2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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