Cul5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cul5
Entrez Gene ID	75717
Full Name	cullin 5
Synonyms	4921514I20Rik,8430423K24Rik,AI852817,C030032G03Rik,C330021I08Rik,VACM-1,VACM1
General protein information	Preferred Names cullin 5 Names cullin-5 CUL-5 vasopressin-activated calcium-mobilizing receptor protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 A5.3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017313680.1	XP_017169169.1	cullin-5 isoform X1
NM_001161618.1	NP_001155090.1	cullin-5 isoform 2
NM_001161618.2	NP_001155090.2	cullin-5 isoform 2
XM_017313680.2	XP_017169169.1	cullin-5 isoform X1
NM_027807.3	NP_082083.2	cullin-5 isoform 1
NM_027807.4	NP_082083.3	cullin-5 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cul5	NP_082083.2
Canis lupus familiaris (dog)	CUL5	XP_005619886.1
Rattus norvegicus (Norway rat)	Cul5	NP_073174.1
Drosophila melanogaster (fruit fly)	Cul-5	NP_651665.2
Xenopus tropicalis (tropical clawed frog)	cul5	NP_001072836.1
Homo sapiens (human)	CUL5	NP_003469.2
	CUL5	XP_001103587.2
Danio rerio (zebrafish)	cul5b	XP_005161301.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002404	XP_312548.5
Caenorhabditis elegans (roundworm)	cul-5	NP_505616.2
Pan troglodytes (chimpanzee)	CUL5	XP_522173.3
Bos taurus (cattle)	CUL5	XP_002693012.1
Gallus gallus (chicken)	CUL5	XP_417163.4

Gene Ontology
Bibliography

Related articles in PubMed

Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury.
Zhu Z, Sun L, Hao R, Jiang H, Qian F, Ye RD
American journal of physiology. Lung cellular and molecular physiology313(1)L104-L114(2017 Jul)

Cutting Edge: A Cullin-5-TRAF6 Interaction Promotes TRAF6 Polyubiquitination and Lipopolysaccharide Signaling.
Zhu Z, Wang L, Hao R, Zhao B, Sun L, Ye RD
Journal of immunology (Baltimore, Md. : 1950)197(1)21-6(2016 07)

Impaired Reelin-Dab1 Signaling Contributes to Neuronal Migration Deficits of Tuberous Sclerosis Complex.
Moon UY, Park JY, Park R, Cho JY, Hughes LJ, McKenna J 3rd, Goetzl L, Cho SH, Crino PB, Gambello MJ, Kim S
Cell reports12(6)965-78(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

neural precursor cell expressed developmentally downregulated protein 8 (Nedd8) modification of Cul-5 is required for its interaction with TRAF6.
Title: Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury.

Our results show that endogenous Cul5 suppresses epithelial cell transformation by several pathways, including inhibition of Src-Cas-induced ruffling through SOCS6
Title: Cullin 5 destabilizes Cas to inhibit Src-dependent cell transformation.

The expression of cullin5 on retinal cells and reduction in the percentage of dead retinal cells induced by L-glutamate suggest that cullin5 has neuroprotective properties in retinal cells.
Title: Cullin5 reduces retinal cell death induced by glutamate toxicity.

The role of Cul5 is to degrade disabled homolog 1 (Dab1) and thus control neuron migration speed and neuron insertion at the top of the cortical plate.
Title: Cullin 5 regulates cortical layering by modulating the speed and duration of Dab1-dependent neuronal migration.

data imply the existence of two distinct subclasses of SOCS proteins with a high affinity for Cullin5, the E3 ligase scaffold, possibly reflecting complete dependence upon ubiquitination for suppression of cytokine signalling
Title: The SOCS box encodes a hierarchy of affinities for Cullin5: implications for ubiquitin ligase formation and cytokine signalling suppression.

The following Cul5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cul5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu84789	NM_027807.4 Latest version!	Mus musculus cullin 5 (Cul5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu84790	NM_001161618.2 Latest version!	Mus musculus cullin 5 (Cul5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu82988	XM_017313680.2 Latest version!	Mus musculus cullin 5 (Cul5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu04630	NM_027807.3 Latest version!	Mus musculus cullin 5 (Cul5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OMu08180	NM_001161618.1 Latest version!	Mus musculus cullin 5 (Cul5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$482.30 $689.00
View Old Accession Versions >>
OMu82988	XM_017313680.1	Mus musculus cullin 5 (Cul5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cul5 ( NM_027807.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082083.3 Mus musculus cullin 5 (Cul5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu84789
Clone ID Related Accession (Same CDS sequence)	NM_027807.4
Accession Version	NM_027807.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Mus musculus(house mouse)
Product	cullin-5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158384.2. On May 20, 2019 this sequence version replaced NM_027807.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK031955.1, SRR7345562.2557259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTCC AGTTTGAAGA CAAGTGGGAC 
TTCATGCATC CAATTGTTTT GAAGCTTTTA CGCCAGGAAT CTGTAACAAA ACAGCAGTGG 
TTTGATCTAT TTTCGGATGT ACATGCTGTC TGTCTCTGGG ATGATAAAGG CTCATCAAAA 
ATTCATCAGG CTTTAAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCTCGTGTA 
CTGAGCCATC AAGATGACAC AGCTTTGCTG AAGGCATATA TTGTTGAATG GCGGAAATTC 
TTCACACAGT GTGATATTTT ACCAAAACCT TTTTGTCAAT TAGAGGTGAC TCTATTGGGT 
AAACAAAGCA GCAATAAAAA ATCAAATATG GAAGACAGTA TTGTTCGAAA GCTCATGCTT 
GATACGTGGA ATGAGTCGAT TTTTTCAAAT ATAAAGAACA GACTCCAGGA CAGTGCAATG 
AAGCTGGTGC ATGCTGAGAG ATTAGGGGAA GCTTTTGATT CCCAGCTGGT CATCGGGGTG 
CGAGAGTCCT ATGTTAATCT TTGCTCCAAC CCCGAGGACA AGCTTCAGAT CTATAGGGAT 
AATTTTGAGA AGGCATACTT GGACTCAACA GAGAGGTTTT ATAGAACACA GGCACCCTCA 
TATTTACAGC AAAATGGTGT GCAGAATTAC ATGAAATATG CAGATGCTAA ATTAAAAGAA 
GAAGAAAAAA GAGCACTCCG ATACTTAGAA ACACGACGAG AGTGTAACTC TGTGGAAGCA 
CTCATGGAAT GCTGTGTAAA TGCGCTGGTG ACCTCCTTTA AAGAGACTAT TTTAGCAGAA 
TGCCAAGGCA TGATCAAGCG AAATGAAACT GAAAAGTTAC ATTTGATGTT TTCCTTGATG 
GACAAAGTTC CTAATGGGAT AGAGCCGATG TTGAAGGACT TGGAGGAGCA TATTATAAGT 
GCGGGCCTAG CAGACATGGT GGCCGCAGCT GAAACCATCA CTACTGACTC TGAGAAGTAT 
GTGGAGCAAT TACTTACACT GTTTAATAGA TTCAGTAAAC TGGTCAAAGA AGCTTTTCAG 
GATGATCCTC GTTTCCTTAC TGCAAGAGAT AAGGCATATA AAGCAGTTGT TAATGATGCT 
ACTATATTTA AACTTGAATT GCCTTTGAAG CAAAAAGGAG TGGGGTTGAA AACTCAGCCT 
GAGTCAAAAT GCCCCGAGTT GCTTGCCAAT TACTGTGACA TGTTGTTAAG GAAAACGCCA 
TTAAGCAAAA AACTAACATC TGAGGAGATT GAAGCAAAGC TTAAAGAAGT GCTCTTGGTA 
CTTAAATATG TACAAAACAA AGATGTTTTT ATGAGGTATC ACAAAGCTCA TCTTACCCGA 
CGTCTCATAT TGGACATCTC TGCTGATAGT GAGATTGAAG AAAACATGGT AGAGTGGCTA 
AGAGAAGTTG GTATGCCAGC AGATTATGTG AACAAGCTTG CTAGAATGTT TCAGGACATA 
AAAGTATCTG AAGACTTGAA CCAAGCTTTT AAGGAAATGC ACAAAAATAA TAAGTTGGCA 
TTACCAGCTG ATTCCGTTAA TATAAAGATT TTGAATGCTG GTGCTTGGTC TAGAAGCTCC 
GAGAAAGTCT TTGTCTCACT TCCTACTGAA CTGGAGGATT TGATACCTGA AGTAGAAGAA 
TTTTACAAAA AAAATCACAG TGGTAGAAAA TTACACTGGC ACCATCTCAT GTCAAATGGA 
ATTATAACAT TTAAAAATGA AGTAGGTCAG TATGATTTGG AAGTAACCAC GTTTCAGTTG 
GCTGTGTTGT TTGCATGGAA CCAAAGGCCT AGAGAGAAAA TCAGCTTTGA AAATCTAAAA 
CTTGCAACGG AACTCCCAGA TGCTGAACTT AGAAGGACTT TATGGTCTTT AGTAGCTTTT 
CCCAAGCTCA AACGGCAAGT TTTGTTGTAT GACCCTCAAG TCAACTCACC CAAAGATTTT 
ACAGAAGGCA CCCTCTTCTC AGTGAACCAG GACTTCAGTC TCATAAAAAA TGCAAAAGTA 
CAGAAAAGGG GGAAAATCAA TTTGATTGGA CGCTTGCAGC TCACTACAGA ACGAATGAGA 
GAAGAAGAAA ATGAAGGGAT AGTCCAACTA AGAATATTAA GAACCCAGGA AGCCATCATA 
CAAATAATGA AAATGAGAAA GAAAATTAGC AATGCCCAGC TGCAGACTGA ATTAGTAGAA 
ATTCTGAAAA ACATGTTCCT GCCTCAGAAG AAGATGATAA AGGAGCAGAT GGAGTGGCTG 
ATTGAACACA GGTACATCCG GAGGGACGAG GCCGACATCA ACACCTTCAT CTACATGGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082083.3
CDS	447..2789
Translation	MATSNLLKNKGSLQFEDKWDFMHPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGSSKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEVTLLGKQSSNKKSNMEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRYIRRDEADINTFIYMA

Target ORF information:

RefSeq Version	NM_027807.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 5 (Cul5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027807.4

ORF Insert Sequence:

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTCC AGTTTGAAGA CAAGTGGGAC 
TTCATGCATC CAATTGTTTT GAAGCTTTTA CGCCAGGAAT CTGTAACAAA ACAGCAGTGG 
TTTGATCTAT TTTCGGATGT ACATGCTGTC TGTCTCTGGG ATGATAAAGG CTCATCAAAA 
ATTCATCAGG CTTTAAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCTCGTGTA 
CTGAGCCATC AAGATGACAC AGCTTTGCTG AAGGCATATA TTGTTGAATG GCGGAAATTC 
TTCACACAGT GTGATATTTT ACCAAAACCT TTTTGTCAAT TAGAGGTGAC TCTATTGGGT 
AAACAAAGCA GCAATAAAAA ATCAAATATG GAAGACAGTA TTGTTCGAAA GCTCATGCTT 
GATACGTGGA ATGAGTCGAT TTTTTCAAAT ATAAAGAACA GACTCCAGGA CAGTGCAATG 
AAGCTGGTGC ATGCTGAGAG ATTAGGGGAA GCTTTTGATT CCCAGCTGGT CATCGGGGTG 
CGAGAGTCCT ATGTTAATCT TTGCTCCAAC CCCGAGGACA AGCTTCAGAT CTATAGGGAT 
AATTTTGAGA AGGCATACTT GGACTCAACA GAGAGGTTTT ATAGAACACA GGCACCCTCA 
TATTTACAGC AAAATGGTGT GCAGAATTAC ATGAAATATG CAGATGCTAA ATTAAAAGAA 
GAAGAAAAAA GAGCACTCCG ATACTTAGAA ACACGACGAG AGTGTAACTC TGTGGAAGCA 
CTCATGGAAT GCTGTGTAAA TGCGCTGGTG ACCTCCTTTA AAGAGACTAT TTTAGCAGAA 
TGCCAAGGCA TGATCAAGCG AAATGAAACT GAAAAGTTAC ATTTGATGTT TTCCTTGATG 
GACAAAGTTC CTAATGGGAT AGAGCCGATG TTGAAGGACT TGGAGGAGCA TATTATAAGT 
GCGGGCCTAG CAGACATGGT GGCCGCAGCT GAAACCATCA CTACTGACTC TGAGAAGTAT 
GTGGAGCAAT TACTTACACT GTTTAATAGA TTCAGTAAAC TGGTCAAAGA AGCTTTTCAG 
GATGATCCTC GTTTCCTTAC TGCAAGAGAT AAGGCATATA AAGCAGTTGT TAATGATGCT 
ACTATATTTA AACTTGAATT GCCTTTGAAG CAAAAAGGAG TGGGGTTGAA AACTCAGCCT 
GAGTCAAAAT GCCCCGAGTT GCTTGCCAAT TACTGTGACA TGTTGTTAAG GAAAACGCCA 
TTAAGCAAAA AACTAACATC TGAGGAGATT GAAGCAAAGC TTAAAGAAGT GCTCTTGGTA 
CTTAAATATG TACAAAACAA AGATGTTTTT ATGAGGTATC ACAAAGCTCA TCTTACCCGA 
CGTCTCATAT TGGACATCTC TGCTGATAGT GAGATTGAAG AAAACATGGT AGAGTGGCTA 
AGAGAAGTTG GTATGCCAGC AGATTATGTG AACAAGCTTG CTAGAATGTT TCAGGACATA 
AAAGTATCTG AAGACTTGAA CCAAGCTTTT AAGGAAATGC ACAAAAATAA TAAGTTGGCA 
TTACCAGCTG ATTCCGTTAA TATAAAGATT TTGAATGCTG GTGCTTGGTC TAGAAGCTCC 
GAGAAAGTCT TTGTCTCACT TCCTACTGAA CTGGAGGATT TGATACCTGA AGTAGAAGAA 
TTTTACAAAA AAAATCACAG TGGTAGAAAA TTACACTGGC ACCATCTCAT GTCAAATGGA 
ATTATAACAT TTAAAAATGA AGTAGGTCAG TATGATTTGG AAGTAACCAC GTTTCAGTTG 
GCTGTGTTGT TTGCATGGAA CCAAAGGCCT AGAGAGAAAA TCAGCTTTGA AAATCTAAAA 
CTTGCAACGG AACTCCCAGA TGCTGAACTT AGAAGGACTT TATGGTCTTT AGTAGCTTTT 
CCCAAGCTCA AACGGCAAGT TTTGTTGTAT GACCCTCAAG TCAACTCACC CAAAGATTTT 
ACAGAAGGCA CCCTCTTCTC AGTGAACCAG GACTTCAGTC TCATAAAAAA TGCAAAAGTA 
CAGAAAAGGG GGAAAATCAA TTTGATTGGA CGCTTGCAGC TCACTACAGA ACGAATGAGA 
GAAGAAGAAA ATGAAGGGAT AGTCCAACTA AGAATATTAA GAACCCAGGA AGCCATCATA 
CAAATAATGA AAATGAGAAA GAAAATTAGC AATGCCCAGC TGCAGACTGA ATTAGTAGAA 
ATTCTGAAAA ACATGTTCCT GCCTCAGAAG AAGATGATAA AGGAGCAGAT GGAGTGGCTG 
ATTGAACACA GGTACATCCG GAGGGACGAG GCCGACATCA ACACCTTCAT CTACATGGCC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul5 ( NM_001161618.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155090.2 Mus musculus cullin 5 (Cul5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu84790
Clone ID Related Accession (Same CDS sequence)	NM_001161618.2
Accession Version	NM_001161618.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Mus musculus(house mouse)
Product	cullin-5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC158384.2. On May 20, 2019 this sequence version replaced NM_001161618.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTCC AGTTTGAAGA CAAGTGGGAC 
TTCATGCATC CAATTGTTTT GAAGCTTTTA CGCCAGGAAT CTGTAACAAA ACAGCAGTGG 
TTTGATCTAT TTTCGGATGT ACATGCTGTC TGTCTCTGGG ATGATAAAGG CTCATCAAAA 
ATTCATCAGG CTTTAAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCTCGTGTA 
CTGAGCCATC AAGATGACAC AGCTTTGCTG AAGGCATATA TTGTTGAATG GCGGAAATTC 
TTCACACAGT GTGATATTTT ACCAAAACCT TTTTGTCAAT TAGAGGTGAC TCTATTGGGT 
AAACAAAGCA GCAATAAAAA ATCAAATATG GAAGACAGTA TTGTTCGAAA GCTCATGCTT 
GATACGTGGA ATGAGTCGAT TTTTTCAAAT ATAAAGAACA GACTCCAGGA CAGTGCAATG 
AAGCTGGTGC ATGCTGAGAG ATTAGGGGAA GCTTTTGATT CCCAGCTGGT CATCGGGGTG 
CGAGAGTCCT ATGTTAATCT TTGCTCCAAC CCCGAGGACA AGCTTCAGAT CTATAGGGAT 
AATTTTGAGA AGGCATACTT GGACTCAACA GAGAGGTTTT ATAGAACACA GGCACCCTCA 
TATTTACAGC AAAATGGTGT GCAGAATTAC ATGAAATATC TCATGGAATG CTGTGTAAAT 
GCGCTGGTGA CCTCCTTTAA AGAGACTATT TTAGCAGAAT GCCAAGGCAT GATCAAGCGA 
AATGAAACTG AAAAGTTACA TTTGATGTTT TCCTTGATGG ACAAAGTTCC TAATGGGATA 
GAGCCGATGT TGAAGGACTT GGAGGAGCAT ATTATAAGTG CGGGCCTAGC AGACATGGTG 
GCCGCAGCTG AAACCATCAC TACTGACTCT GAGAAGTATG TGGAGCAATT ACTTACACTG 
TTTAATAGAT TCAGTAAACT GGTCAAAGAA GCTTTTCAGG ATGATCCTCG TTTCCTTACT 
GCAAGAGATA AGGCATATAA AGCAGTTGTT AATGATGCTA CTATATTTAA ACTTGAATTG 
CCTTTGAAGC AAAAAGGAGT GGGGTTGAAA ACTCAGCCTG AGTCAAAATG CCCCGAGTTG 
CTTGCCAATT ACTGTGACAT GTTGTTAAGG AAAACGCCAT TAAGCAAAAA ACTAACATCT 
GAGGAGATTG AAGCAAAGCT TAAAGAAGTG CTCTTGGTAC TTAAATATGT ACAAAACAAA 
GATGTTTTTA TGAGGTATCA CAAAGCTCAT CTTACCCGAC GTCTCATATT GGACATCTCT 
GCTGATAGTG AGATTGAAGA AAACATGGTA GAGTGGCTAA GAGAAGTTGG TATGCCAGCA 
GATTATGTGA ACAAGCTTGC TAGAATGTTT CAGGACATAA AAGTATCTGA AGACTTGAAC 
CAAGCTTTTA AGGAAATGCA CAAAAATAAT AAGTTGGCAT TACCAGCTGA TTCCGTTAAT 
ATAAAGATTT TGAATGCTGG TGCTTGGTCT AGAAGCTCCG AGAAAGTCTT TGTCTCACTT 
CCTACTGAAC TGGAGGATTT GATACCTGAA GTAGAAGAAT TTTACAAAAA AAATCACAGT 
GGTAGAAAAT TACACTGGCA CCATCTCATG TCAAATGGAA TTATAACATT TAAAAATGAA 
GTAGGTCAGT ATGATTTGGA AGTAACCACG TTTCAGTTGG CTGTGTTGTT TGCATGGAAC 
CAAAGGCCTA GAGAGAAAAT CAGCTTTGAA AATCTAAAAC TTGCAACGGA ACTCCCAGAT 
GCTGAACTTA GAAGGACTTT ATGGTCTTTA GTAGCTTTTC CCAAGCTCAA ACGGCAAGTT 
TTGTTGTATG ACCCTCAAGT CAACTCACCC AAAGATTTTA CAGAAGGCAC CCTCTTCTCA 
GTGAACCAGG ACTTCAGTCT CATAAAAAAT GCAAAAGTAC AGAAAAGGGG GAAAATCAAT 
TTGATTGGAC GCTTGCAGCT CACTACAGAA CGAATGAGAG AAGAAGAAAA TGAAGGGATA 
GTCCAACTAA GAATATTAAG AACCCAGGAA GCCATCATAC AAATAATGAA AATGAGAAAG 
AAAATTAGCA ATGCCCAGCT GCAGACTGAA TTAGTAGAAA TTCTGAAAAA CATGTTCCTG 
CCTCAGAAGA AGATGATAAA GGAGCAGATG GAGTGGCTGA TTGAACACAG GTACATCCGG 
AGGGACGAGG CCGACATCAA CACCTTCATC TACATGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155090.2
CDS	447..2708
Translation	MATSNLLKNKGSLQFEDKWDFMHPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGSSKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEVTLLGKQSSNKKSNMEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYLMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRYIRRDEADINTFIYMA

Target ORF information:

RefSeq Version	NM_001161618.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 5 (Cul5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161618.2

ORF Insert Sequence:

ATGGCGACGT CTAATCTGTT AAAGAATAAA GGTTCTCTCC AGTTTGAAGA CAAGTGGGAC 
TTCATGCATC CAATTGTTTT GAAGCTTTTA CGCCAGGAAT CTGTAACAAA ACAGCAGTGG 
TTTGATCTAT TTTCGGATGT ACATGCTGTC TGTCTCTGGG ATGATAAAGG CTCATCAAAA 
ATTCATCAGG CTTTAAAAGA AGATATTCTT GAGTTTATTA AGCAAGCACA GGCTCGTGTA 
CTGAGCCATC AAGATGACAC AGCTTTGCTG AAGGCATATA TTGTTGAATG GCGGAAATTC 
TTCACACAGT GTGATATTTT ACCAAAACCT TTTTGTCAAT TAGAGGTGAC TCTATTGGGT 
AAACAAAGCA GCAATAAAAA ATCAAATATG GAAGACAGTA TTGTTCGAAA GCTCATGCTT 
GATACGTGGA ATGAGTCGAT TTTTTCAAAT ATAAAGAACA GACTCCAGGA CAGTGCAATG 
AAGCTGGTGC ATGCTGAGAG ATTAGGGGAA GCTTTTGATT CCCAGCTGGT CATCGGGGTG 
CGAGAGTCCT ATGTTAATCT TTGCTCCAAC CCCGAGGACA AGCTTCAGAT CTATAGGGAT 
AATTTTGAGA AGGCATACTT GGACTCAACA GAGAGGTTTT ATAGAACACA GGCACCCTCA 
TATTTACAGC AAAATGGTGT GCAGAATTAC ATGAAATATC TCATGGAATG CTGTGTAAAT 
GCGCTGGTGA CCTCCTTTAA AGAGACTATT TTAGCAGAAT GCCAAGGCAT GATCAAGCGA 
AATGAAACTG AAAAGTTACA TTTGATGTTT TCCTTGATGG ACAAAGTTCC TAATGGGATA 
GAGCCGATGT TGAAGGACTT GGAGGAGCAT ATTATAAGTG CGGGCCTAGC AGACATGGTG 
GCCGCAGCTG AAACCATCAC TACTGACTCT GAGAAGTATG TGGAGCAATT ACTTACACTG 
TTTAATAGAT TCAGTAAACT GGTCAAAGAA GCTTTTCAGG ATGATCCTCG TTTCCTTACT 
GCAAGAGATA AGGCATATAA AGCAGTTGTT AATGATGCTA CTATATTTAA ACTTGAATTG 
CCTTTGAAGC AAAAAGGAGT GGGGTTGAAA ACTCAGCCTG AGTCAAAATG CCCCGAGTTG 
CTTGCCAATT ACTGTGACAT GTTGTTAAGG AAAACGCCAT TAAGCAAAAA ACTAACATCT 
GAGGAGATTG AAGCAAAGCT TAAAGAAGTG CTCTTGGTAC TTAAATATGT ACAAAACAAA 
GATGTTTTTA TGAGGTATCA CAAAGCTCAT CTTACCCGAC GTCTCATATT GGACATCTCT 
GCTGATAGTG AGATTGAAGA AAACATGGTA GAGTGGCTAA GAGAAGTTGG TATGCCAGCA 
GATTATGTGA ACAAGCTTGC TAGAATGTTT CAGGACATAA AAGTATCTGA AGACTTGAAC 
CAAGCTTTTA AGGAAATGCA CAAAAATAAT AAGTTGGCAT TACCAGCTGA TTCCGTTAAT 
ATAAAGATTT TGAATGCTGG TGCTTGGTCT AGAAGCTCCG AGAAAGTCTT TGTCTCACTT 
CCTACTGAAC TGGAGGATTT GATACCTGAA GTAGAAGAAT TTTACAAAAA AAATCACAGT 
GGTAGAAAAT TACACTGGCA CCATCTCATG TCAAATGGAA TTATAACATT TAAAAATGAA 
GTAGGTCAGT ATGATTTGGA AGTAACCACG TTTCAGTTGG CTGTGTTGTT TGCATGGAAC 
CAAAGGCCTA GAGAGAAAAT CAGCTTTGAA AATCTAAAAC TTGCAACGGA ACTCCCAGAT 
GCTGAACTTA GAAGGACTTT ATGGTCTTTA GTAGCTTTTC CCAAGCTCAA ACGGCAAGTT 
TTGTTGTATG ACCCTCAAGT CAACTCACCC AAAGATTTTA CAGAAGGCAC CCTCTTCTCA 
GTGAACCAGG ACTTCAGTCT CATAAAAAAT GCAAAAGTAC AGAAAAGGGG GAAAATCAAT 
TTGATTGGAC GCTTGCAGCT CACTACAGAA CGAATGAGAG AAGAAGAAAA TGAAGGGATA 
GTCCAACTAA GAATATTAAG AACCCAGGAA GCCATCATAC AAATAATGAA AATGAGAAAG 
AAAATTAGCA ATGCCCAGCT GCAGACTGAA TTAGTAGAAA TTCTGAAAAA CATGTTCCTG 
CCTCAGAAGA AGATGATAAA GGAGCAGATG GAGTGGCTGA TTGAACACAG GTACATCCGG 
AGGGACGAGG CCGACATCAA CACCTTCATC TACATGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul5 ( XM_017313680.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169169.1 Mus musculus cullin 5 (Cul5), transcript variant X1, mRNA.

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CloneID	OMu82988
Clone ID Related Accession (Same CDS sequence)	XM_017313680.1 , XM_017313680.2
Accession Version	XM_017313680.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cullin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GTATTGTTCG AAAGCTCATG CTTGATACGT GGAATGAGTC GATTTTTTCA 
AATATAAAGA ACAGACTCCA GGACAGTGCA ATGAAGCTGG TGCATGCTGA GAGATTAGGG 
GAAGCTTTTG ATTCCCAGCT GGTCATCGGG GTGCGAGAGT CCTATGTTAA TCTTTGCTCC 
AACCCCGAGG ACAAGCTTCA GATCTATAGG GATAATTTTG AGAAGGCATA CTTGGACTCA 
ACAGAGAGGT TTTATAGAAC ACAGGCACCC TCATATTTAC AGCAAAATGG TGTGCAGAAT 
TACATGAAAT ATGCAGATGC TAAATTAAAA GAAGAAGAAA AAAGAGCACT CCGATACTTA 
GAAACACGAC GAGAGTGTAA CTCTGTGGAA GCACTCATGG AATGCTGTGT AAATGCGCTG 
GTGACCTCCT TTAAAGAGAC TATTTTAGCA GAATGCCAAG GCATGATCAA GCGAAATGAA 
ACTGAAAAGT TACATTTGAT GTTTTCCTTG ATGGACAAAG TTCCTAATGG GATAGAGCCG 
ATGTTGAAGG ACTTGGAGGA GCATATTATA AGTGCGGGCC TAGCAGACAT GGTGGCCGCA 
GCTGAAACCA TCACTACTGA CTCTGAGAAG TATGTGGAGC AATTACTTAC ACTGTTTAAT 
AGATTCAGTA AACTGGTCAA AGAAGCTTTT CAGGATGATC CTCGTTTCCT TACTGCAAGA 
GATAAGGCAT ATAAAGCAGT TGTTAATGAT GCTACTATAT TTAAACTTGA ATTGCCTTTG 
AAGCAAAAAG GAGTGGGGTT GAAAACTCAG CCTGAGTCAA AATGCCCCGA GTTGCTTGCC 
AATTACTGTG ACATGTTGTT AAGGAAAACG CCATTAAGCA AAAAACTAAC ATCTGAGGAG 
ATTGAAGCAA AGCTTAAAGA AGTGCTCTTG GTACTTAAAT ATGTACAAAA CAAAGATGTT 
TTTATGAGGT ATCACAAAGC TCATCTTACC CGACGTCTCA TATTGGACAT CTCTGCTGAT 
AGTGAGATTG AAGAAAACAT GGTAGAGTGG CTAAGAGAAG TTGGTATGCC AGCAGATTAT 
GTGAACAAGC TTGCTAGAAT GTTTCAGGAC ATAAAAGTAT CTGAAGACTT GAACCAAGCT 
TTTAAGGAAA TGCACAAAAA TAATAAGTTG GCATTACCAG CTGATTCCGT TAATATAAAG 
ATTTTGAATG CTGGTGCTTG GTCTAGAAGC TCCGAGAAAG TCTTTGTCTC ACTTCCTACT 
GAACTGGAGG ATTTGATACC TGAAGTAGAA GAATTTTACA AAAAAAATCA CAGTGGTAGA 
AAATTACACT GGCACCATCT CATGTCAAAT GGAATTATAA CATTTAAAAA TGAAGTAGGT 
CAGTATGATT TGGAAGTAAC CACGTTTCAG TTGGCTGTGT TGTTTGCATG GAACCAAAGG 
CCTAGAGAGA AAATCAGCTT TGAAAATCTA AAACTTGCAA CGGAACTCCC AGATGCTGAA 
CTTAGAAGGA CTTTATGGTC TTTAGTAGCT TTTCCCAAGC TCAAACGGCA AGTTTTGTTG 
TATGACCCTC AAGTCAACTC ACCCAAAGAT TTTACAGAAG GCACCCTCTT CTCAGTGAAC 
CAGGACTTCA GTCTCATAAA AAATGCAAAA GTACAGAAAA GGGGGAAAAT CAATTTGATT 
GGACGCTTGC AGCTCACTAC AGAACGAATG AGAGAAGAAG AAAATGAAGG GATAGTCCAA 
CTAAGAATAT TAAGAACCCA GGAAGCCATC ATACAAATAA TGAAAATGAG AAAGAAAATT 
AGCAATGCCC AGCTGCAGAC TGAATTAGTA GAAATTCTGA AAAACATGTT CCTGCCTCAG 
AAGAAGATGA TAAAGGAGCA GATGGAGTGG CTGATTGAAC ACAGGTACAT CCGGAGGGAC 
GAGGCCGACA TCAACACCTT CATCTACATG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169169.1
CDS	592..2547
Translation	MEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRYIRRDEADINTFIYMA

Target ORF information:

RefSeq Version	XM_017313680.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 5 (Cul5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313680.1

ORF Insert Sequence:

ATGGAAGACA GTATTGTTCG AAAGCTCATG CTTGATACGT GGAATGAGTC GATTTTTTCA 
AATATAAAGA ACAGACTCCA GGACAGTGCA ATGAAGCTGG TGCATGCTGA GAGATTAGGG 
GAAGCTTTTG ATTCCCAGCT GGTCATCGGG GTGCGAGAGT CCTATGTTAA TCTTTGCTCC 
AACCCCGAGG ACAAGCTTCA GATCTATAGG GATAATTTTG AGAAGGCATA CTTGGACTCA 
ACAGAGAGGT TTTATAGAAC ACAGGCACCC TCATATTTAC AGCAAAATGG TGTGCAGAAT 
TACATGAAAT ATGCAGATGC TAAATTAAAA GAAGAAGAAA AAAGAGCACT CCGATACTTA 
GAAACACGAC GAGAGTGTAA CTCTGTGGAA GCACTCATGG AATGCTGTGT AAATGCGCTG 
GTGACCTCCT TTAAAGAGAC TATTTTAGCA GAATGCCAAG GCATGATCAA GCGAAATGAA 
ACTGAAAAGT TACATTTGAT GTTTTCCTTG ATGGACAAAG TTCCTAATGG GATAGAGCCG 
ATGTTGAAGG ACTTGGAGGA GCATATTATA AGTGCGGGCC TAGCAGACAT GGTGGCCGCA 
GCTGAAACCA TCACTACTGA CTCTGAGAAG TATGTGGAGC AATTACTTAC ACTGTTTAAT 
AGATTCAGTA AACTGGTCAA AGAAGCTTTT CAGGATGATC CTCGTTTCCT TACTGCAAGA 
GATAAGGCAT ATAAAGCAGT TGTTAATGAT GCTACTATAT TTAAACTTGA ATTGCCTTTG 
AAGCAAAAAG GAGTGGGGTT GAAAACTCAG CCTGAGTCAA AATGCCCCGA GTTGCTTGCC 
AATTACTGTG ACATGTTGTT AAGGAAAACG CCATTAAGCA AAAAACTAAC ATCTGAGGAG 
ATTGAAGCAA AGCTTAAAGA AGTGCTCTTG GTACTTAAAT ATGTACAAAA CAAAGATGTT 
TTTATGAGGT ATCACAAAGC TCATCTTACC CGACGTCTCA TATTGGACAT CTCTGCTGAT 
AGTGAGATTG AAGAAAACAT GGTAGAGTGG CTAAGAGAAG TTGGTATGCC AGCAGATTAT 
GTGAACAAGC TTGCTAGAAT GTTTCAGGAC ATAAAAGTAT CTGAAGACTT GAACCAAGCT 
TTTAAGGAAA TGCACAAAAA TAATAAGTTG GCATTACCAG CTGATTCCGT TAATATAAAG 
ATTTTGAATG CTGGTGCTTG GTCTAGAAGC TCCGAGAAAG TCTTTGTCTC ACTTCCTACT 
GAACTGGAGG ATTTGATACC TGAAGTAGAA GAATTTTACA AAAAAAATCA CAGTGGTAGA 
AAATTACACT GGCACCATCT CATGTCAAAT GGAATTATAA CATTTAAAAA TGAAGTAGGT 
CAGTATGATT TGGAAGTAAC CACGTTTCAG TTGGCTGTGT TGTTTGCATG GAACCAAAGG 
CCTAGAGAGA AAATCAGCTT TGAAAATCTA AAACTTGCAA CGGAACTCCC AGATGCTGAA 
CTTAGAAGGA CTTTATGGTC TTTAGTAGCT TTTCCCAAGC TCAAACGGCA AGTTTTGTTG 
TATGACCCTC AAGTCAACTC ACCCAAAGAT TTTACAGAAG GCACCCTCTT CTCAGTGAAC 
CAGGACTTCA GTCTCATAAA AAATGCAAAA GTACAGAAAA GGGGGAAAAT CAATTTGATT 
GGACGCTTGC AGCTCACTAC AGAACGAATG AGAGAAGAAG AAAATGAAGG GATAGTCCAA 
CTAAGAATAT TAAGAACCCA GGAAGCCATC ATACAAATAA TGAAAATGAG AAAGAAAATT 
AGCAATGCCC AGCTGCAGAC TGAATTAGTA GAAATTCTGA AAAACATGTT CCTGCCTCAG 
AAGAAGATGA TAAAGGAGCA GATGGAGTGG CTGATTGAAC ACAGGTACAT CCGGAGGGAC 
GAGGCCGACA TCAACACCTT CATCTACATG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul5 ( XM_017313680.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017169169.1 Mus musculus cullin 5 (Cul5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu82988
Clone ID Related Accession (Same CDS sequence)	XM_017313680.1 , XM_017313680.2
Accession Version	XM_017313680.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cullin-5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA GTATTGTTCG AAAGCTCATG CTTGATACGT GGAATGAGTC GATTTTTTCA 
AATATAAAGA ACAGACTCCA GGACAGTGCA ATGAAGCTGG TGCATGCTGA GAGATTAGGG 
GAAGCTTTTG ATTCCCAGCT GGTCATCGGG GTGCGAGAGT CCTATGTTAA TCTTTGCTCC 
AACCCCGAGG ACAAGCTTCA GATCTATAGG GATAATTTTG AGAAGGCATA CTTGGACTCA 
ACAGAGAGGT TTTATAGAAC ACAGGCACCC TCATATTTAC AGCAAAATGG TGTGCAGAAT 
TACATGAAAT ATGCAGATGC TAAATTAAAA GAAGAAGAAA AAAGAGCACT CCGATACTTA 
GAAACACGAC GAGAGTGTAA CTCTGTGGAA GCACTCATGG AATGCTGTGT AAATGCGCTG 
GTGACCTCCT TTAAAGAGAC TATTTTAGCA GAATGCCAAG GCATGATCAA GCGAAATGAA 
ACTGAAAAGT TACATTTGAT GTTTTCCTTG ATGGACAAAG TTCCTAATGG GATAGAGCCG 
ATGTTGAAGG ACTTGGAGGA GCATATTATA AGTGCGGGCC TAGCAGACAT GGTGGCCGCA 
GCTGAAACCA TCACTACTGA CTCTGAGAAG TATGTGGAGC AATTACTTAC ACTGTTTAAT 
AGATTCAGTA AACTGGTCAA AGAAGCTTTT CAGGATGATC CTCGTTTCCT TACTGCAAGA 
GATAAGGCAT ATAAAGCAGT TGTTAATGAT GCTACTATAT TTAAACTTGA ATTGCCTTTG 
AAGCAAAAAG GAGTGGGGTT GAAAACTCAG CCTGAGTCAA AATGCCCCGA GTTGCTTGCC 
AATTACTGTG ACATGTTGTT AAGGAAAACG CCATTAAGCA AAAAACTAAC ATCTGAGGAG 
ATTGAAGCAA AGCTTAAAGA AGTGCTCTTG GTACTTAAAT ATGTACAAAA CAAAGATGTT 
TTTATGAGGT ATCACAAAGC TCATCTTACC CGACGTCTCA TATTGGACAT CTCTGCTGAT 
AGTGAGATTG AAGAAAACAT GGTAGAGTGG CTAAGAGAAG TTGGTATGCC AGCAGATTAT 
GTGAACAAGC TTGCTAGAAT GTTTCAGGAC ATAAAAGTAT CTGAAGACTT GAACCAAGCT 
TTTAAGGAAA TGCACAAAAA TAATAAGTTG GCATTACCAG CTGATTCCGT TAATATAAAG 
ATTTTGAATG CTGGTGCTTG GTCTAGAAGC TCCGAGAAAG TCTTTGTCTC ACTTCCTACT 
GAACTGGAGG ATTTGATACC TGAAGTAGAA GAATTTTACA AAAAAAATCA CAGTGGTAGA 
AAATTACACT GGCACCATCT CATGTCAAAT GGAATTATAA CATTTAAAAA TGAAGTAGGT 
CAGTATGATT TGGAAGTAAC CACGTTTCAG TTGGCTGTGT TGTTTGCATG GAACCAAAGG 
CCTAGAGAGA AAATCAGCTT TGAAAATCTA AAACTTGCAA CGGAACTCCC AGATGCTGAA 
CTTAGAAGGA CTTTATGGTC TTTAGTAGCT TTTCCCAAGC TCAAACGGCA AGTTTTGTTG 
TATGACCCTC AAGTCAACTC ACCCAAAGAT TTTACAGAAG GCACCCTCTT CTCAGTGAAC 
CAGGACTTCA GTCTCATAAA AAATGCAAAA GTACAGAAAA GGGGGAAAAT CAATTTGATT 
GGACGCTTGC AGCTCACTAC AGAACGAATG AGAGAAGAAG AAAATGAAGG GATAGTCCAA 
CTAAGAATAT TAAGAACCCA GGAAGCCATC ATACAAATAA TGAAAATGAG AAAGAAAATT 
AGCAATGCCC AGCTGCAGAC TGAATTAGTA GAAATTCTGA AAAACATGTT CCTGCCTCAG 
AAGAAGATGA TAAAGGAGCA GATGGAGTGG CTGATTGAAC ACAGGTACAT CCGGAGGGAC 
GAGGCCGACA TCAACACCTT CATCTACATG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017169169.1
CDS	682..2637
Translation	MEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRYIRRDEADINTFIYMA

Target ORF information:

RefSeq Version	XM_017313680.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 5 (Cul5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017313680.2

ORF Insert Sequence:

ATGGAAGACA GTATTGTTCG AAAGCTCATG CTTGATACGT GGAATGAGTC GATTTTTTCA 
AATATAAAGA ACAGACTCCA GGACAGTGCA ATGAAGCTGG TGCATGCTGA GAGATTAGGG 
GAAGCTTTTG ATTCCCAGCT GGTCATCGGG GTGCGAGAGT CCTATGTTAA TCTTTGCTCC 
AACCCCGAGG ACAAGCTTCA GATCTATAGG GATAATTTTG AGAAGGCATA CTTGGACTCA 
ACAGAGAGGT TTTATAGAAC ACAGGCACCC TCATATTTAC AGCAAAATGG TGTGCAGAAT 
TACATGAAAT ATGCAGATGC TAAATTAAAA GAAGAAGAAA AAAGAGCACT CCGATACTTA 
GAAACACGAC GAGAGTGTAA CTCTGTGGAA GCACTCATGG AATGCTGTGT AAATGCGCTG 
GTGACCTCCT TTAAAGAGAC TATTTTAGCA GAATGCCAAG GCATGATCAA GCGAAATGAA 
ACTGAAAAGT TACATTTGAT GTTTTCCTTG ATGGACAAAG TTCCTAATGG GATAGAGCCG 
ATGTTGAAGG ACTTGGAGGA GCATATTATA AGTGCGGGCC TAGCAGACAT GGTGGCCGCA 
GCTGAAACCA TCACTACTGA CTCTGAGAAG TATGTGGAGC AATTACTTAC ACTGTTTAAT 
AGATTCAGTA AACTGGTCAA AGAAGCTTTT CAGGATGATC CTCGTTTCCT TACTGCAAGA 
GATAAGGCAT ATAAAGCAGT TGTTAATGAT GCTACTATAT TTAAACTTGA ATTGCCTTTG 
AAGCAAAAAG GAGTGGGGTT GAAAACTCAG CCTGAGTCAA AATGCCCCGA GTTGCTTGCC 
AATTACTGTG ACATGTTGTT AAGGAAAACG CCATTAAGCA AAAAACTAAC ATCTGAGGAG 
ATTGAAGCAA AGCTTAAAGA AGTGCTCTTG GTACTTAAAT ATGTACAAAA CAAAGATGTT 
TTTATGAGGT ATCACAAAGC TCATCTTACC CGACGTCTCA TATTGGACAT CTCTGCTGAT 
AGTGAGATTG AAGAAAACAT GGTAGAGTGG CTAAGAGAAG TTGGTATGCC AGCAGATTAT 
GTGAACAAGC TTGCTAGAAT GTTTCAGGAC ATAAAAGTAT CTGAAGACTT GAACCAAGCT 
TTTAAGGAAA TGCACAAAAA TAATAAGTTG GCATTACCAG CTGATTCCGT TAATATAAAG 
ATTTTGAATG CTGGTGCTTG GTCTAGAAGC TCCGAGAAAG TCTTTGTCTC ACTTCCTACT 
GAACTGGAGG ATTTGATACC TGAAGTAGAA GAATTTTACA AAAAAAATCA CAGTGGTAGA 
AAATTACACT GGCACCATCT CATGTCAAAT GGAATTATAA CATTTAAAAA TGAAGTAGGT 
CAGTATGATT TGGAAGTAAC CACGTTTCAG TTGGCTGTGT TGTTTGCATG GAACCAAAGG 
CCTAGAGAGA AAATCAGCTT TGAAAATCTA AAACTTGCAA CGGAACTCCC AGATGCTGAA 
CTTAGAAGGA CTTTATGGTC TTTAGTAGCT TTTCCCAAGC TCAAACGGCA AGTTTTGTTG 
TATGACCCTC AAGTCAACTC ACCCAAAGAT TTTACAGAAG GCACCCTCTT CTCAGTGAAC 
CAGGACTTCA GTCTCATAAA AAATGCAAAA GTACAGAAAA GGGGGAAAAT CAATTTGATT 
GGACGCTTGC AGCTCACTAC AGAACGAATG AGAGAAGAAG AAAATGAAGG GATAGTCCAA 
CTAAGAATAT TAAGAACCCA GGAAGCCATC ATACAAATAA TGAAAATGAG AAAGAAAATT 
AGCAATGCCC AGCTGCAGAC TGAATTAGTA GAAATTCTGA AAAACATGTT CCTGCCTCAG 
AAGAAGATGA TAAAGGAGCA GATGGAGTGG CTGATTGAAC ACAGGTACAT CCGGAGGGAC 
GAGGCCGACA TCAACACCTT CATCTACATG GCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul5 ( NM_027807.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082083.2 Mus musculus cullin 5 (Cul5), transcript variant 1, mRNA.

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CloneID	OMu04630
Clone ID Related Accession (Same CDS sequence)	NM_027807.3
Accession Version	NM_027807.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	cullin-5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ045576.1, AK046030.1 and AK080305.1. On Jun 6, 2009 this sequence version replaced NM_027807.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.273921.1, AK046030.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCTCTC TCGCGTGGGC AGGCCGGGGT GACCATGTAG CTGGAAAGCC CGAGGAAGCA 
CGGCTGCCCG GGACGAGCTC GGCGCTGACG GCACGCCGCT CCGGCGTCCC CCGCATCCCC 
CGCCGCGGCC TGCGGGGTCC TGCTGGGAAC CCCGGCCTCT CGAGGAGGCC TGGCCCCGAG 
CGCCGCCAAG TCTCGCCCCG TCTCGCGAGA GTCCAAGTTG AGAACATGGC GACGTCTAAT 
CTGTTAAAGA ATAAAGGTTC TCTCCAGTTT GAAGACAAGT GGGACTTCAT GCATCCAATT 
GTTTTGAAGC TTTTACGCCA GGAATCTGTA ACAAAACAGC AGTGGTTTGA TCTATTTTCG 
GATGTACATG CTGTCTGTCT CTGGGATGAT AAAGGCTCAT CAAAAATTCA TCAGGCTTTA 
AAAGAAGATA TTCTTGAGTT TATTAAGCAA GCACAGGCTC GTGTACTGAG CCATCAAGAT 
GACACAGCTT TGCTGAAGGC ATATATTGTT GAATGGCGGA AATTCTTCAC ACAGTGTGAT 
ATTTTACCAA AACCTTTTTG TCAATTAGAG GTGACTCTAT TGGGTAAACA AAGCAGCAAT 
AAAAAATCAA ATATGGAAGA CAGTATTGTT CGAAAGCTCA TGCTTGATAC GTGGAATGAG 
TCGATTTTTT CAAATATAAA GAACAGACTC CAGGACAGTG CAATGAAGCT GGTGCATGCT 
GAGAGATTAG GGGAAGCTTT TGATTCCCAG CTGGTCATCG GGGTGCGAGA GTCCTATGTT 
AATCTTTGCT CCAACCCCGA GGACAAGCTT CAGATCTATA GGGATAATTT TGAGAAGGCA 
TACTTGGACT CAACAGAGAG GTTTTATAGA ACACAGGCAC CCTCATATTT ACAGCAAAAT 
GGTGTGCAGA ATTACATGAA ATATGCAGAT GCTAAATTAA AAGAAGAAGA AAAAAGAGCA 
CTCCGATACT TAGAAACACG ACGAGAGTGT AACTCTGTGG AAGCACTCAT GGAATGCTGT 
GTAAATGCGC TGGTGACCTC CTTTAAAGAG ACTATTTTAG CAGAATGCCA AGGCATGATC 
AAGCGAAATG AAACTGAAAA GTTACATTTG ATGTTTTCCT TGATGGACAA AGTTCCTAAT 
GGGATAGAGC CGATGTTGAA GGACTTGGAG GAGCATATTA TAAGTGCGGG CCTAGCAGAC 
ATGGTGGCCG CAGCTGAAAC CATCACTACT GACTCTGAGA AGTATGTGGA GCAATTACTT 
ACACTGTTTA ATAGATTCAG TAAACTGGTC AAAGAAGCTT TTCAGGATGA TCCTCGTTTC 
CTTACTGCAA GAGATAAGGC ATATAAAGCA GTTGTTAATG ATGCTACTAT ATTTAAACTT 
GAATTGCCTT TGAAGCAAAA AGGAGTGGGG TTGAAAACTC AGCCTGAGTC AAAATGCCCC 
GAGTTGCTTG CCAATTACTG TGACATGTTG TTAAGGAAAA CGCCATTAAG CAAAAAACTA 
ACATCTGAGG AGATTGAAGC AAAGCTTAAA GAAGTGCTCT TGGTACTTAA ATATGTACAA 
AACAAAGATG TTTTTATGAG GTATCACAAA GCTCATCTTA CCCGACGTCT CATATTGGAC 
ATCTCTGCTG ATAGTGAGAT TGAAGAAAAC ATGGTAGAGT GGCTAAGAGA AGTTGGTATG 
CCAGCAGATT ATGTGAACAA GCTTGCTAGA ATGTTTCAGG ACATAAAAGT ATCTGAAGAC 
TTGAACCAAG CTTTTAAGGA AATGCACAAA AATAATAAGT TGGCATTACC AGCTGATTCC 
GTTAATATAA AGATTTTGAA TGCTGGTGCT TGGTCTAGAA GCTCCGAGAA AGTCTTTGTC 
TCACTTCCTA CTGAACTGGA GGATTTGATA CCTGAAGTAG AAGAATTTTA CAAAAAAAAT 
CACAGTGGTA GAAAATTACA CTGGCACCAT CTCATGTCAA ATGGAATTAT AACATTTAAA 
AATGAAGTAG GTCAGTATGA TTTGGAAGTA ACCACGTTTC AGTTGGCTGT GTTGTTTGCA 
TGGAACCAAA GGCCTAGAGA GAAAATCAGC TTTGAAAATC TAAAACTTGC AACGGAACTC 
CCAGATGCTG AACTTAGAAG GACTTTATGG TCTTTAGTAG CTTTTCCCAA GCTCAAACGG 
CAAGTTTTGT TGTATGACCC TCAAGTCAAC TCACCCAAAG ATTTTACAGA AGGCACCCTC 
TTCTCAGTGA ACCAGGACTT CAGTCTCATA AAAAATGCAA AAGTACAGAA AAGGGGGAAA 
ATCAATTTGA TTGGACGCTT GCAGCTCACT ACAGAACGAA TGAGAGAAGA AGAAAATGAA 
GGGATAGTCC AACTAAGAAT ATTAAGAACC CAGGAAGCCA TCATACAAAT AATGAAAATG 
AGAAAGAAAA TTAGCAATGC CCAGCTGCAG ACTGAATTAG TAGAAATTCT GAAAAACATG 
TTCCTGCCTC AGAAGAAGAT GATAAAGGAG CAGATGGAGT GGCTGATTGA ACACAGGTAC 
ATCCGGAGGG ACGAGGCCGA CATCAACACC TTCATCTACA TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082083.2
CDS	222..2789
Translation	MRSLAWAGRGDHVAGKPEEARLPGTSSALTARRSGVPRIPRRGLRGPAGNPGLSRRPGPERRQVSPRLARVQVENMATSNLLKNKGSLQFEDKWDFMHPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGSSKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEVTLLGKQSSNKKSNMEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRYIRRDEADINTFIYMA

Target ORF information:

RefSeq Version	NM_027807.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 5 (Cul5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027807.3

ORF Insert Sequence:

ATGCGCTCTC TCGCGTGGGC AGGCCGGGGT GACCATGTAG CTGGAAAGCC CGAGGAAGCA 
CGGCTGCCCG GGACGAGCTC GGCGCTGACG GCACGCCGCT CCGGCGTCCC CCGCATCCCC 
CGCCGCGGCC TGCGGGGTCC TGCTGGGAAC CCCGGCCTCT CGAGGAGGCC TGGCCCCGAG 
CGCCGCCAAG TCTCGCCCCG TCTCGCGAGA GTCCAAGTTG AGAACATGGC GACGTCTAAT 
CTGTTAAAGA ATAAAGGTTC TCTCCAGTTT GAAGACAAGT GGGACTTCAT GCATCCAATT 
GTTTTGAAGC TTTTACGCCA GGAATCTGTA ACAAAACAGC AGTGGTTTGA TCTATTTTCG 
GATGTACATG CTGTCTGTCT CTGGGATGAT AAAGGCTCAT CAAAAATTCA TCAGGCTTTA 
AAAGAAGATA TTCTTGAGTT TATTAAGCAA GCACAGGCTC GTGTACTGAG CCATCAAGAT 
GACACAGCTT TGCTGAAGGC ATATATTGTT GAATGGCGGA AATTCTTCAC ACAGTGTGAT 
ATTTTACCAA AACCTTTTTG TCAATTAGAG GTGACTCTAT TGGGTAAACA AAGCAGCAAT 
AAAAAATCAA ATATGGAAGA CAGTATTGTT CGAAAGCTCA TGCTTGATAC GTGGAATGAG 
TCGATTTTTT CAAATATAAA GAACAGACTC CAGGACAGTG CAATGAAGCT GGTGCATGCT 
GAGAGATTAG GGGAAGCTTT TGATTCCCAG CTGGTCATCG GGGTGCGAGA GTCCTATGTT 
AATCTTTGCT CCAACCCCGA GGACAAGCTT CAGATCTATA GGGATAATTT TGAGAAGGCA 
TACTTGGACT CAACAGAGAG GTTTTATAGA ACACAGGCAC CCTCATATTT ACAGCAAAAT 
GGTGTGCAGA ATTACATGAA ATATGCAGAT GCTAAATTAA AAGAAGAAGA AAAAAGAGCA 
CTCCGATACT TAGAAACACG ACGAGAGTGT AACTCTGTGG AAGCACTCAT GGAATGCTGT 
GTAAATGCGC TGGTGACCTC CTTTAAAGAG ACTATTTTAG CAGAATGCCA AGGCATGATC 
AAGCGAAATG AAACTGAAAA GTTACATTTG ATGTTTTCCT TGATGGACAA AGTTCCTAAT 
GGGATAGAGC CGATGTTGAA GGACTTGGAG GAGCATATTA TAAGTGCGGG CCTAGCAGAC 
ATGGTGGCCG CAGCTGAAAC CATCACTACT GACTCTGAGA AGTATGTGGA GCAATTACTT 
ACACTGTTTA ATAGATTCAG TAAACTGGTC AAAGAAGCTT TTCAGGATGA TCCTCGTTTC 
CTTACTGCAA GAGATAAGGC ATATAAAGCA GTTGTTAATG ATGCTACTAT ATTTAAACTT 
GAATTGCCTT TGAAGCAAAA AGGAGTGGGG TTGAAAACTC AGCCTGAGTC AAAATGCCCC 
GAGTTGCTTG CCAATTACTG TGACATGTTG TTAAGGAAAA CGCCATTAAG CAAAAAACTA 
ACATCTGAGG AGATTGAAGC AAAGCTTAAA GAAGTGCTCT TGGTACTTAA ATATGTACAA 
AACAAAGATG TTTTTATGAG GTATCACAAA GCTCATCTTA CCCGACGTCT CATATTGGAC 
ATCTCTGCTG ATAGTGAGAT TGAAGAAAAC ATGGTAGAGT GGCTAAGAGA AGTTGGTATG 
CCAGCAGATT ATGTGAACAA GCTTGCTAGA ATGTTTCAGG ACATAAAAGT ATCTGAAGAC 
TTGAACCAAG CTTTTAAGGA AATGCACAAA AATAATAAGT TGGCATTACC AGCTGATTCC 
GTTAATATAA AGATTTTGAA TGCTGGTGCT TGGTCTAGAA GCTCCGAGAA AGTCTTTGTC 
TCACTTCCTA CTGAACTGGA GGATTTGATA CCTGAAGTAG AAGAATTTTA CAAAAAAAAT 
CACAGTGGTA GAAAATTACA CTGGCACCAT CTCATGTCAA ATGGAATTAT AACATTTAAA 
AATGAAGTAG GTCAGTATGA TTTGGAAGTA ACCACGTTTC AGTTGGCTGT GTTGTTTGCA 
TGGAACCAAA GGCCTAGAGA GAAAATCAGC TTTGAAAATC TAAAACTTGC AACGGAACTC 
CCAGATGCTG AACTTAGAAG GACTTTATGG TCTTTAGTAG CTTTTCCCAA GCTCAAACGG 
CAAGTTTTGT TGTATGACCC TCAAGTCAAC TCACCCAAAG ATTTTACAGA AGGCACCCTC 
TTCTCAGTGA ACCAGGACTT CAGTCTCATA AAAAATGCAA AAGTACAGAA AAGGGGGAAA 
ATCAATTTGA TTGGACGCTT GCAGCTCACT ACAGAACGAA TGAGAGAAGA AGAAAATGAA 
GGGATAGTCC AACTAAGAAT ATTAAGAACC CAGGAAGCCA TCATACAAAT AATGAAAATG 
AGAAAGAAAA TTAGCAATGC CCAGCTGCAG ACTGAATTAG TAGAAATTCT GAAAAACATG 
TTCCTGCCTC AGAAGAAGAT GATAAAGGAG CAGATGGAGT GGCTGATTGA ACACAGGTAC 
ATCCGGAGGG ACGAGGCCGA CATCAACACC TTCATCTACA TGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cul5 ( NM_001161618.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001155090.1 Mus musculus cullin 5 (Cul5), transcript variant 2, mRNA.

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CloneID	OMu08180
Clone ID Related Accession (Same CDS sequence)	NM_001161618.1
Accession Version	NM_001161618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	cullin-5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ045576.1, AK046030.1, CV559336.1 and AK080305.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGCTCTC TCGCGTGGGC AGGCCGGGGT GACCATGTAG CTGGAAAGCC CGAGGAAGCA 
CGGCTGCCCG GGACGAGCTC GGCGCTGACG GCACGCCGCT CCGGCGTCCC CCGCATCCCC 
CGCCGCGGCC TGCGGGGTCC TGCTGGGAAC CCCGGCCTCT CGAGGAGGCC TGGCCCCGAG 
CGCCGCCAAG TCTCGCCCCG TCTCGCGAGA GTCCAAGTTG AGAACATGGC GACGTCTAAT 
CTGTTAAAGA ATAAAGGTTC TCTCCAGTTT GAAGACAAGT GGGACTTCAT GCATCCAATT 
GTTTTGAAGC TTTTACGCCA GGAATCTGTA ACAAAACAGC AGTGGTTTGA TCTATTTTCG 
GATGTACATG CTGTCTGTCT CTGGGATGAT AAAGGCTCAT CAAAAATTCA TCAGGCTTTA 
AAAGAAGATA TTCTTGAGTT TATTAAGCAA GCACAGGCTC GTGTACTGAG CCATCAAGAT 
GACACAGCTT TGCTGAAGGC ATATATTGTT GAATGGCGGA AATTCTTCAC ACAGTGTGAT 
ATTTTACCAA AACCTTTTTG TCAATTAGAG GTGACTCTAT TGGGTAAACA AAGCAGCAAT 
AAAAAATCAA ATATGGAAGA CAGTATTGTT CGAAAGCTCA TGCTTGATAC GTGGAATGAG 
TCGATTTTTT CAAATATAAA GAACAGACTC CAGGACAGTG CAATGAAGCT GGTGCATGCT 
GAGAGATTAG GGGAAGCTTT TGATTCCCAG CTGGTCATCG GGGTGCGAGA GTCCTATGTT 
AATCTTTGCT CCAACCCCGA GGACAAGCTT CAGATCTATA GGGATAATTT TGAGAAGGCA 
TACTTGGACT CAACAGAGAG GTTTTATAGA ACACAGGCAC CCTCATATTT ACAGCAAAAT 
GGTGTGCAGA ATTACATGAA ATATCTCATG GAATGCTGTG TAAATGCGCT GGTGACCTCC 
TTTAAAGAGA CTATTTTAGC AGAATGCCAA GGCATGATCA AGCGAAATGA AACTGAAAAG 
TTACATTTGA TGTTTTCCTT GATGGACAAA GTTCCTAATG GGATAGAGCC GATGTTGAAG 
GACTTGGAGG AGCATATTAT AAGTGCGGGC CTAGCAGACA TGGTGGCCGC AGCTGAAACC 
ATCACTACTG ACTCTGAGAA GTATGTGGAG CAATTACTTA CACTGTTTAA TAGATTCAGT 
AAACTGGTCA AAGAAGCTTT TCAGGATGAT CCTCGTTTCC TTACTGCAAG AGATAAGGCA 
TATAAAGCAG TTGTTAATGA TGCTACTATA TTTAAACTTG AATTGCCTTT GAAGCAAAAA 
GGAGTGGGGT TGAAAACTCA GCCTGAGTCA AAATGCCCCG AGTTGCTTGC CAATTACTGT 
GACATGTTGT TAAGGAAAAC GCCATTAAGC AAAAAACTAA CATCTGAGGA GATTGAAGCA 
AAGCTTAAAG AAGTGCTCTT GGTACTTAAA TATGTACAAA ACAAAGATGT TTTTATGAGG 
TATCACAAAG CTCATCTTAC CCGACGTCTC ATATTGGACA TCTCTGCTGA TAGTGAGATT 
GAAGAAAACA TGGTAGAGTG GCTAAGAGAA GTTGGTATGC CAGCAGATTA TGTGAACAAG 
CTTGCTAGAA TGTTTCAGGA CATAAAAGTA TCTGAAGACT TGAACCAAGC TTTTAAGGAA 
ATGCACAAAA ATAATAAGTT GGCATTACCA GCTGATTCCG TTAATATAAA GATTTTGAAT 
GCTGGTGCTT GGTCTAGAAG CTCCGAGAAA GTCTTTGTCT CACTTCCTAC TGAACTGGAG 
GATTTGATAC CTGAAGTAGA AGAATTTTAC AAAAAAAATC ACAGTGGTAG AAAATTACAC 
TGGCACCATC TCATGTCAAA TGGAATTATA ACATTTAAAA ATGAAGTAGG TCAGTATGAT 
TTGGAAGTAA CCACGTTTCA GTTGGCTGTG TTGTTTGCAT GGAACCAAAG GCCTAGAGAG 
AAAATCAGCT TTGAAAATCT AAAACTTGCA ACGGAACTCC CAGATGCTGA ACTTAGAAGG 
ACTTTATGGT CTTTAGTAGC TTTTCCCAAG CTCAAACGGC AAGTTTTGTT GTATGACCCT 
CAAGTCAACT CACCCAAAGA TTTTACAGAA GGCACCCTCT TCTCAGTGAA CCAGGACTTC 
AGTCTCATAA AAAATGCAAA AGTACAGAAA AGGGGGAAAA TCAATTTGAT TGGACGCTTG 
CAGCTCACTA CAGAACGAAT GAGAGAAGAA GAAAATGAAG GGATAGTCCA ACTAAGAATA 
TTAAGAACCC AGGAAGCCAT CATACAAATA ATGAAAATGA GAAAGAAAAT TAGCAATGCC 
CAGCTGCAGA CTGAATTAGT AGAAATTCTG AAAAACATGT TCCTGCCTCA GAAGAAGATG 
ATAAAGGAGC AGATGGAGTG GCTGATTGAA CACAGGTACA TCCGGAGGGA CGAGGCCGAC 
ATCAACACCT TCATCTACAT GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155090.1
CDS	222..2708
Translation	MRSLAWAGRGDHVAGKPEEARLPGTSSALTARRSGVPRIPRRGLRGPAGNPGLSRRPGPERRQVSPRLARVQVENMATSNLLKNKGSLQFEDKWDFMHPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGSSKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEVTLLGKQSSNKKSNMEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYLMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYDPQVNSPKDFTEGTLFSVNQDFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQMEWLIEHRYIRRDEADINTFIYMA

Target ORF information:

RefSeq Version	NM_001161618.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cullin 5 (Cul5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161618.1

ORF Insert Sequence:

ATGCGCTCTC TCGCGTGGGC AGGCCGGGGT GACCATGTAG CTGGAAAGCC CGAGGAAGCA 
CGGCTGCCCG GGACGAGCTC GGCGCTGACG GCACGCCGCT CCGGCGTCCC CCGCATCCCC 
CGCCGCGGCC TGCGGGGTCC TGCTGGGAAC CCCGGCCTCT CGAGGAGGCC TGGCCCCGAG 
CGCCGCCAAG TCTCGCCCCG TCTCGCGAGA GTCCAAGTTG AGAACATGGC GACGTCTAAT 
CTGTTAAAGA ATAAAGGTTC TCTCCAGTTT GAAGACAAGT GGGACTTCAT GCATCCAATT 
GTTTTGAAGC TTTTACGCCA GGAATCTGTA ACAAAACAGC AGTGGTTTGA TCTATTTTCG 
GATGTACATG CTGTCTGTCT CTGGGATGAT AAAGGCTCAT CAAAAATTCA TCAGGCTTTA 
AAAGAAGATA TTCTTGAGTT TATTAAGCAA GCACAGGCTC GTGTACTGAG CCATCAAGAT 
GACACAGCTT TGCTGAAGGC ATATATTGTT GAATGGCGGA AATTCTTCAC ACAGTGTGAT 
ATTTTACCAA AACCTTTTTG TCAATTAGAG GTGACTCTAT TGGGTAAACA AAGCAGCAAT 
AAAAAATCAA ATATGGAAGA CAGTATTGTT CGAAAGCTCA TGCTTGATAC GTGGAATGAG 
TCGATTTTTT CAAATATAAA GAACAGACTC CAGGACAGTG CAATGAAGCT GGTGCATGCT 
GAGAGATTAG GGGAAGCTTT TGATTCCCAG CTGGTCATCG GGGTGCGAGA GTCCTATGTT 
AATCTTTGCT CCAACCCCGA GGACAAGCTT CAGATCTATA GGGATAATTT TGAGAAGGCA 
TACTTGGACT CAACAGAGAG GTTTTATAGA ACACAGGCAC CCTCATATTT ACAGCAAAAT 
GGTGTGCAGA ATTACATGAA ATATCTCATG GAATGCTGTG TAAATGCGCT GGTGACCTCC 
TTTAAAGAGA CTATTTTAGC AGAATGCCAA GGCATGATCA AGCGAAATGA AACTGAAAAG 
TTACATTTGA TGTTTTCCTT GATGGACAAA GTTCCTAATG GGATAGAGCC GATGTTGAAG 
GACTTGGAGG AGCATATTAT AAGTGCGGGC CTAGCAGACA TGGTGGCCGC AGCTGAAACC 
ATCACTACTG ACTCTGAGAA GTATGTGGAG CAATTACTTA CACTGTTTAA TAGATTCAGT 
AAACTGGTCA AAGAAGCTTT TCAGGATGAT CCTCGTTTCC TTACTGCAAG AGATAAGGCA 
TATAAAGCAG TTGTTAATGA TGCTACTATA TTTAAACTTG AATTGCCTTT GAAGCAAAAA 
GGAGTGGGGT TGAAAACTCA GCCTGAGTCA AAATGCCCCG AGTTGCTTGC CAATTACTGT 
GACATGTTGT TAAGGAAAAC GCCATTAAGC AAAAAACTAA CATCTGAGGA GATTGAAGCA 
AAGCTTAAAG AAGTGCTCTT GGTACTTAAA TATGTACAAA ACAAAGATGT TTTTATGAGG 
TATCACAAAG CTCATCTTAC CCGACGTCTC ATATTGGACA TCTCTGCTGA TAGTGAGATT 
GAAGAAAACA TGGTAGAGTG GCTAAGAGAA GTTGGTATGC CAGCAGATTA TGTGAACAAG 
CTTGCTAGAA TGTTTCAGGA CATAAAAGTA TCTGAAGACT TGAACCAAGC TTTTAAGGAA 
ATGCACAAAA ATAATAAGTT GGCATTACCA GCTGATTCCG TTAATATAAA GATTTTGAAT 
GCTGGTGCTT GGTCTAGAAG CTCCGAGAAA GTCTTTGTCT CACTTCCTAC TGAACTGGAG 
GATTTGATAC CTGAAGTAGA AGAATTTTAC AAAAAAAATC ACAGTGGTAG AAAATTACAC 
TGGCACCATC TCATGTCAAA TGGAATTATA ACATTTAAAA ATGAAGTAGG TCAGTATGAT 
TTGGAAGTAA CCACGTTTCA GTTGGCTGTG TTGTTTGCAT GGAACCAAAG GCCTAGAGAG 
AAAATCAGCT TTGAAAATCT AAAACTTGCA ACGGAACTCC CAGATGCTGA ACTTAGAAGG 
ACTTTATGGT CTTTAGTAGC TTTTCCCAAG CTCAAACGGC AAGTTTTGTT GTATGACCCT 
CAAGTCAACT CACCCAAAGA TTTTACAGAA GGCACCCTCT TCTCAGTGAA CCAGGACTTC 
AGTCTCATAA AAAATGCAAA AGTACAGAAA AGGGGGAAAA TCAATTTGAT TGGACGCTTG 
CAGCTCACTA CAGAACGAAT GAGAGAAGAA GAAAATGAAG GGATAGTCCA ACTAAGAATA 
TTAAGAACCC AGGAAGCCAT CATACAAATA ATGAAAATGA GAAAGAAAAT TAGCAATGCC 
CAGCTGCAGA CTGAATTAGT AGAAATTCTG AAAAACATGT TCCTGCCTCA GAAGAAGATG 
ATAAAGGAGC AGATGGAGTG GCTGATTGAA CACAGGTACA TCCGGAGGGA CGAGGCCGAC 
ATCAACACCT TCATCTACAT GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nedd8 modification of Cullin-5 regulates lipopolysaccharide-induced acute lung injury.
American journal of physiology. Lung cellular and molecular physiology313(1)L104-L114(2017 Jul)
Zhu Z,Sun L,Hao R,Jiang H,Qian F,Ye RD

Cutting Edge: A Cullin-5-TRAF6 Interaction Promotes TRAF6 Polyubiquitination and Lipopolysaccharide Signaling.
Journal of immunology (Baltimore, Md. : 1950)197(1)21-6(2016 07)
Zhu Z,Wang L,Hao R,Zhao B,Sun L,Ye RD

Impaired Reelin-Dab1 Signaling Contributes to Neuronal Migration Deficits of Tuberous Sclerosis Complex.
Cell reports12(6)965-78(2015 Aug)
Moon UY,Park JY,Park R,Cho JY,Hughes LJ,McKenna J 3rd,Goetzl L,Cho SH,Crino PB,Gambello MJ,Kim S

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Cul5 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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