Wbp2nl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wbp2nl
Entrez Gene ID	74716
Full Name	WBP2 N-terminal like
Synonyms	4930521I23Rik,PAWP
General protein information	Preferred Names WBP2 N-terminal like Names postacrosomal sheath WW domain-binding protein WW domain-binding protein 2-like
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017316772.2	XP_017172261.1	postacrosomal sheath WW domain-binding protein isoform X1
NM_029066.1	NP_083342.1	postacrosomal sheath WW domain-binding protein
XM_017316772.1	XP_017172261.1	postacrosomal sheath WW domain-binding protein isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wbp2nl	NP_083342.1
Rattus norvegicus (Norway rat)	Wbp2nl	XP_001067725.2
	LOC709297	XP_002798421.1
Canis lupus familiaris (dog)	WBP2NL	XP_005625874.1
Bos taurus (cattle)	WBP2NL	NP_001075910.1
Homo sapiens (human)	WBP2NL	NP_689826.2
Pan troglodytes (chimpanzee)	WBP2NL	XP_003317337.1

Gene Ontology
Bibliography

Related articles in PubMed

Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation.
Satouh Y, Nozawa K, Ikawa M
Biology of reproduction93(4)94(2015 Oct)

Sperm-derived WW domain-binding protein, PAWP, elicits calcium oscillations and oocyte activation in humans and mice.
Aarabi M, Balakier H, Bashar S, Moskovtsev SI, Sutovsky P, Librach CL, Oko R
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(10)4434-40(2014 Oct)

Sperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes.
Nomikos M, Sanders JR, Theodoridou M, Kashir J, Matthews E, Nounesis G, Lai FA, Swann K
Molecular human reproduction20(10)938-47(2014 Oct)

Expression analysis of PAWP during mouse embryonic stem cell-based spermatogenesis in vitro.
Nourashrafeddin S, Aarabi M, Miryounesi M, Ebrahimzadeh-Vesal R, Zarghami N, Modarressi MH, Nouri M
In vitro cellular & developmental biology. Animal50(5)475-81(2014)

The postacrosomal assembly of sperm head protein, PAWP, is independent of acrosome formation and dependent on microtubular manchette transport.
Wu AT, Sutovsky P, Xu W, van der Spoel AC, Platt FM, Oko R
Developmental biology312(2)471-83(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PAWP does not play an essential role in mouse fertilization
Title: Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation.

The data suggest that sperm-derived PAWP is not involved in triggering Ca(2+) oscillations at fertilization in mammalian oocytes.
Title: Sperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes.

These results indicate that PAWP plays a role in spermatogenesis and germ cell development. Moreover, we suggest PAWP as one of the markers that could be looked in ESC studies as a confirmed testis-specific gene
Title: Expression analysis of PAWP during mouse embryonic stem cell-based spermatogenesis in vitro.

The postacrosomal assembly of sperm head protein, PAWP, is independent of acrosome formation and dependent on microtubular manchette transport
Title: The postacrosomal assembly of sperm head protein, PAWP, is independent of acrosome formation and dependent on microtubular manchette transport.

The following Wbp2nl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wbp2nl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06486	NM_029066.1 Latest version!	Mus musculus WBP2 N-terminal like (Wbp2nl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OMu79192	XM_017316772.2 Latest version!	Mus musculus WBP2 N-terminal like (Wbp2nl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu79192	XM_017316772.1	Mus musculus WBP2 N-terminal like (Wbp2nl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wbp2nl ( NM_029066.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083342.1 Mus musculus WBP2 N-terminal like (Wbp2nl), mRNA.

Price & Availability↑

CloneID	OMu06486
Clone ID Related Accession (Same CDS sequence)	NM_029066.1
Accession Version	NM_029066.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	postacrosomal sheath WW domain-binding protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC119520.2. On or before Nov 9, 2006 this sequence version replaced XM_905693.2, XM_987274.1, XM_001001745.1. ##Evidence-Data-START## Transcript exon combination :: BC119520.2, AK015863.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGTGA ACCAGAACCA TACAGTGGAC CGCCGTTGGG CCGCCATCCC TCATGGTGAA 
AGTCTCTTGA AGAAATGTTC AGAAGTGGAC CTCTCCTTCC CCCAGTCACC GCCAGGCTCC 
AACCTCTTTA GTGGTACAAA GAGGGGGGCT CTGTTTCTCA CCTCATACCG GGTGATTTTT 
GTGACTTCGC GTGCAGACAA CGATCCCATG TTTTCTTTTA CGATGCCATT TCATCTAATG 
AATAACTGCA CTGTTGAACA ACCAATCTTT GGTGCAAACT ACATTAAAGG GACAATTCAG 
GCAGCTCCAG ACGGTGGCTG GGAAGGTTCT GCTACTTTTA AAATAGTCTT CCGGAAAGGA 
GGTGCTATCG ACTTTGCCCA GTTGATGGCC AAAGCTGCCT CTGCGGCTGC CCAAGGAGTT 
CCACTACGAG TTGCAAGCTT CTGGATGGGC CCTCTAGGAA TTTATGTCAT CACTGGGGAC 
AGGAACATGT ATGCCCCACA GGCATACCAA GTTGCCTATG GAGCCCCACC TGCGGGATAT 
GGGGCCTCAC CCGTGGGATA CGGAGTCCCA TCTGCAGGGT ATGGAGCCCC ACCTGCGGGG 
TACGGAGCCC CGCCAGTGGG TTATGTAGCC CCATCTCCCG GATACGATGT CCTGCCTCCT 
GGATACGGAG CTGTGAGATA TGGCTCCCCT CCTCCTCTAT ATGTAGCTAC CCCAATGGGC 
TATGGAGTTC CACCTCCTGG ATATGGACCT CCACCTGTGA GATACGGATC CCCACCTCCT 
GGATATGAAG CCCCCACTAT GGAGTATGGA GCCCAACCTC CCAGATACGG AACTACACCT 
ATGGGATCCG GTTCCCCACC TCCCAGATAT GAAGCCCCGC CTATGGGGTA TGGAACTCCT 
CCTTCAGGGC GTGAGTCCAT ACCTCCTGGA TCAAGGGCCA CGTCTGTAGC ACAGGAAGCT 
CCACCTGCTG GGTCTGAAGC AGGCCATCCC ATGTCTGTGG CAGTCCAGAA TCCTGAATTC 
CAGGCATCTT TTCCCTCTAC CTCGTCCTCA CAAGTCCACT CTCCCCGCTC TAAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083342.1
CDS	24..1103
Translation	MAVNQNHTVDRRWAAIPHGESLLKKCSEVDLSFPQSPPGSNLFSGTKRGALFLTSYRVIFVTSRADNDPMFSFTMPFHLMNNCTVEQPIFGANYIKGTIQAAPDGGWEGSATFKIVFRKGGAIDFAQLMAKAASAAAQGVPLRVASFWMGPLGIYVITGDRNMYAPQAYQVAYGAPPAGYGASPVGYGVPSAGYGAPPAGYGAPPVGYVAPSPGYDVLPPGYGAVRYGSPPPLYVATPMGYGVPPPGYGPPPVRYGSPPPGYEAPTMEYGAQPPRYGTTPMGSGSPPPRYEAPPMGYGTPPSGRESIPPGSRATSVAQEAPPAGSEAGHPMSVAVQNPEFQASFPSTSSSQVHSPRSKM

Target ORF information:

RefSeq Version	NM_029066.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WBP2 N-terminal like (Wbp2nl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029066.1

ORF Insert Sequence:

ATGGCAGTGA ACCAGAACCA TACAGTGGAC CGCCGTTGGG CCGCCATCCC TCATGGTGAA 
AGTCTCTTGA AGAAATGTTC AGAAGTGGAC CTCTCCTTCC CCCAGTCACC GCCAGGCTCC 
AACCTCTTTA GTGGTACAAA GAGGGGGGCT CTGTTTCTCA CCTCATACCG GGTGATTTTT 
GTGACTTCGC GTGCAGACAA CGATCCCATG TTTTCTTTTA CGATGCCATT TCATCTAATG 
AATAACTGCA CTGTTGAACA ACCAATCTTT GGTGCAAACT ACATTAAAGG GACAATTCAG 
GCAGCTCCAG ACGGTGGCTG GGAAGGTTCT GCTACTTTTA AAATAGTCTT CCGGAAAGGA 
GGTGCTATCG ACTTTGCCCA GTTGATGGCC AAAGCTGCCT CTGCGGCTGC CCAAGGAGTT 
CCACTACGAG TTGCAAGCTT CTGGATGGGC CCTCTAGGAA TTTATGTCAT CACTGGGGAC 
AGGAACATGT ATGCCCCACA GGCATACCAA GTTGCCTATG GAGCCCCACC TGCGGGATAT 
GGGGCCTCAC CCGTGGGATA CGGAGTCCCA TCTGCAGGGT ATGGAGCCCC ACCTGCGGGG 
TACGGAGCCC CGCCAGTGGG TTATGTAGCC CCATCTCCCG GATACGATGT CCTGCCTCCT 
GGATACGGAG CTGTGAGATA TGGCTCCCCT CCTCCTCTAT ATGTAGCTAC CCCAATGGGC 
TATGGAGTTC CACCTCCTGG ATATGGACCT CCACCTGTGA GATACGGATC CCCACCTCCT 
GGATATGAAG CCCCCACTAT GGAGTATGGA GCCCAACCTC CCAGATACGG AACTACACCT 
ATGGGATCCG GTTCCCCACC TCCCAGATAT GAAGCCCCGC CTATGGGGTA TGGAACTCCT 
CCTTCAGGGC GTGAGTCCAT ACCTCCTGGA TCAAGGGCCA CGTCTGTAGC ACAGGAAGCT 
CCACCTGCTG GGTCTGAAGC AGGCCATCCC ATGTCTGTGG CAGTCCAGAA TCCTGAATTC 
CAGGCATCTT TTCCCTCTAC CTCGTCCTCA CAAGTCCACT CTCCCCGCTC TAAGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wbp2nl ( XM_017316772.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172261.1 Mus musculus WBP2 N-terminal like (Wbp2nl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79192
Clone ID Related Accession (Same CDS sequence)	XM_017316772.2 , XM_017316772.1
Accession Version	XM_017316772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	postacrosomal sheath WW domain-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017316772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGA ACCAGAACCA TACAGTGGAC CGCCGTTGGG CCGCCATCCC TCATGGTGAA 
AGGTGCCTGA GGGGACTTTC AGGCTGCTGT CAAGGAGGGA GGAGATGCTC AGGTCTCTTG 
AAGAAATGTT CAGAAGTGGA CCTCTCCTTC CCCCAGTCAC CGCCAGGCTC CAACCTCTTT 
AGTGGTACAA AGAGGGGGGC TCTGTTTCTC ACCTCATACC GGGTGATTTT TGTGACTTCG 
CGTGCAGACA ACGATCCCAT GTTTTCTTTT ACGATGCCAT TTCATCTAAT GAATAACTGC 
ACTGTTGAAC AACCAATCTT TGGTGCAAAC TACATTAAAG GGACAATTCA GGCAGCTCCA 
GACGGTGGCT GGGAAGGTTC TGCTACTTTT AAAATAGTCT TCCGGAAAGG AGGTGCTATC 
GACTTTGCCC AGTTGATGGC CAAAGCTGCC TCTGCGGCTG CCCAAGGAGT TCCACTACGA 
GTTGCAAGCT TCTGGATGGG CCCTCTAGGA ATTTATGTCA TCACTGGGGA CAGGAACATG 
TATGCCCCAC AGGCATACCA AGTTGCCTAT GGAGCCCCAC CTGCGGGATA TGGGGCCTCA 
CCCGTGGGAT ACGGAGTCCC ATCTGCAGGG TATGGAGCCC CACCTGCGGG GTACGGAGCC 
CCGCCAGTGG GTTATGTAGC CCCATCTCCC GGATACGATG TCCTGCCTCC TGGATACGGA 
GCTGTGAGAT ATGGCTCCCC TCCTCCTCTA TATGTAGCTA CCCCAATGGG CTATGGAGTT 
CCACCTCCTG GATATGGACC TCCACCTGTG AGATACGGAT CCCCACCTCC TGGATATGAA 
GCCCCCACTA TGGAGTATGG AGCCCAACCT CCCAGATACG GAACTACACC TATGGGATCC 
GGTTCCCCAC CTCCCAGATA TGAAGCCCCG CCTATGGGGT ATGGAACTCC TCCTTCAGGG 
CGTGAGTCCA TACCTCCTGG ATCAAGGGCC ACGTCTGTAG CACAGGAAGC TCCACCTGCT 
GGGTCTGAAG CAGGCCATCC CATGTCTGTG GCAGTCCAGA ATCCTGAATT CCAGGCATCT 
TTTCCCTCTA CCTCGTCCTC ACAAGTCCAC TCTCCCCGCT CTAAGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172261.1
CDS	83..1213
Translation	MAVNQNHTVDRRWAAIPHGERCLRGLSGCCQGGRRCSGLLKKCSEVDLSFPQSPPGSNLFSGTKRGALFLTSYRVIFVTSRADNDPMFSFTMPFHLMNNCTVEQPIFGANYIKGTIQAAPDGGWEGSATFKIVFRKGGAIDFAQLMAKAASAAAQGVPLRVASFWMGPLGIYVITGDRNMYAPQAYQVAYGAPPAGYGASPVGYGVPSAGYGAPPAGYGAPPVGYVAPSPGYDVLPPGYGAVRYGSPPPLYVATPMGYGVPPPGYGPPPVRYGSPPPGYEAPTMEYGAQPPRYGTTPMGSGSPPPRYEAPPMGYGTPPSGRESIPPGSRATSVAQEAPPAGSEAGHPMSVAVQNPEFQASFPSTSSSQVHSPRSKM

Target ORF information:

RefSeq Version	XM_017316772.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus WBP2 N-terminal like (Wbp2nl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316772.2

ORF Insert Sequence:

ATGGCAGTGA ACCAGAACCA TACAGTGGAC CGCCGTTGGG CCGCCATCCC TCATGGTGAA 
AGGTGCCTGA GGGGACTTTC AGGCTGCTGT CAAGGAGGGA GGAGATGCTC AGGTCTCTTG 
AAGAAATGTT CAGAAGTGGA CCTCTCCTTC CCCCAGTCAC CGCCAGGCTC CAACCTCTTT 
AGTGGTACAA AGAGGGGGGC TCTGTTTCTC ACCTCATACC GGGTGATTTT TGTGACTTCG 
CGTGCAGACA ACGATCCCAT GTTTTCTTTT ACGATGCCAT TTCATCTAAT GAATAACTGC 
ACTGTTGAAC AACCAATCTT TGGTGCAAAC TACATTAAAG GGACAATTCA GGCAGCTCCA 
GACGGTGGCT GGGAAGGTTC TGCTACTTTT AAAATAGTCT TCCGGAAAGG AGGTGCTATC 
GACTTTGCCC AGTTGATGGC CAAAGCTGCC TCTGCGGCTG CCCAAGGAGT TCCACTACGA 
GTTGCAAGCT TCTGGATGGG CCCTCTAGGA ATTTATGTCA TCACTGGGGA CAGGAACATG 
TATGCCCCAC AGGCATACCA AGTTGCCTAT GGAGCCCCAC CTGCGGGATA TGGGGCCTCA 
CCCGTGGGAT ACGGAGTCCC ATCTGCAGGG TATGGAGCCC CACCTGCGGG GTACGGAGCC 
CCGCCAGTGG GTTATGTAGC CCCATCTCCC GGATACGATG TCCTGCCTCC TGGATACGGA 
GCTGTGAGAT ATGGCTCCCC TCCTCCTCTA TATGTAGCTA CCCCAATGGG CTATGGAGTT 
CCACCTCCTG GATATGGACC TCCACCTGTG AGATACGGAT CCCCACCTCC TGGATATGAA 
GCCCCCACTA TGGAGTATGG AGCCCAACCT CCCAGATACG GAACTACACC TATGGGATCC 
GGTTCCCCAC CTCCCAGATA TGAAGCCCCG CCTATGGGGT ATGGAACTCC TCCTTCAGGG 
CGTGAGTCCA TACCTCCTGG ATCAAGGGCC ACGTCTGTAG CACAGGAAGC TCCACCTGCT 
GGGTCTGAAG CAGGCCATCC CATGTCTGTG GCAGTCCAGA ATCCTGAATT CCAGGCATCT 
TTTCCCTCTA CCTCGTCCTC ACAAGTCCAC TCTCCCCGCT CTAAGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wbp2nl ( XM_017316772.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017172261.1 Mus musculus WBP2 N-terminal like (Wbp2nl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu79192
Clone ID Related Accession (Same CDS sequence)	XM_017316772.2 , XM_017316772.1
Accession Version	XM_017316772.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	postacrosomal sheath WW domain-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000081.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGTGA ACCAGAACCA TACAGTGGAC CGCCGTTGGG CCGCCATCCC TCATGGTGAA 
AGGTGCCTGA GGGGACTTTC AGGCTGCTGT CAAGGAGGGA GGAGATGCTC AGGTCTCTTG 
AAGAAATGTT CAGAAGTGGA CCTCTCCTTC CCCCAGTCAC CGCCAGGCTC CAACCTCTTT 
AGTGGTACAA AGAGGGGGGC TCTGTTTCTC ACCTCATACC GGGTGATTTT TGTGACTTCG 
CGTGCAGACA ACGATCCCAT GTTTTCTTTT ACGATGCCAT TTCATCTAAT GAATAACTGC 
ACTGTTGAAC AACCAATCTT TGGTGCAAAC TACATTAAAG GGACAATTCA GGCAGCTCCA 
GACGGTGGCT GGGAAGGTTC TGCTACTTTT AAAATAGTCT TCCGGAAAGG AGGTGCTATC 
GACTTTGCCC AGTTGATGGC CAAAGCTGCC TCTGCGGCTG CCCAAGGAGT TCCACTACGA 
GTTGCAAGCT TCTGGATGGG CCCTCTAGGA ATTTATGTCA TCACTGGGGA CAGGAACATG 
TATGCCCCAC AGGCATACCA AGTTGCCTAT GGAGCCCCAC CTGCGGGATA TGGGGCCTCA 
CCCGTGGGAT ACGGAGTCCC ATCTGCAGGG TATGGAGCCC CACCTGCGGG GTACGGAGCC 
CCGCCAGTGG GTTATGTAGC CCCATCTCCC GGATACGATG TCCTGCCTCC TGGATACGGA 
GCTGTGAGAT ATGGCTCCCC TCCTCCTCTA TATGTAGCTA CCCCAATGGG CTATGGAGTT 
CCACCTCCTG GATATGGACC TCCACCTGTG AGATACGGAT CCCCACCTCC TGGATATGAA 
GCCCCCACTA TGGAGTATGG AGCCCAACCT CCCAGATACG GAACTACACC TATGGGATCC 
GGTTCCCCAC CTCCCAGATA TGAAGCCCCG CCTATGGGGT ATGGAACTCC TCCTTCAGGG 
CGTGAGTCCA TACCTCCTGG ATCAAGGGCC ACGTCTGTAG CACAGGAAGC TCCACCTGCT 
GGGTCTGAAG CAGGCCATCC CATGTCTGTG GCAGTCCAGA ATCCTGAATT CCAGGCATCT 
TTTCCCTCTA CCTCGTCCTC ACAAGTCCAC TCTCCCCGCT CTAAGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017172261.1
CDS	84..1214
Translation	MAVNQNHTVDRRWAAIPHGERCLRGLSGCCQGGRRCSGLLKKCSEVDLSFPQSPPGSNLFSGTKRGALFLTSYRVIFVTSRADNDPMFSFTMPFHLMNNCTVEQPIFGANYIKGTIQAAPDGGWEGSATFKIVFRKGGAIDFAQLMAKAASAAAQGVPLRVASFWMGPLGIYVITGDRNMYAPQAYQVAYGAPPAGYGASPVGYGVPSAGYGAPPAGYGAPPVGYVAPSPGYDVLPPGYGAVRYGSPPPLYVATPMGYGVPPPGYGPPPVRYGSPPPGYEAPTMEYGAQPPRYGTTPMGSGSPPPRYEAPPMGYGTPPSGRESIPPGSRATSVAQEAPPAGSEAGHPMSVAVQNPEFQASFPSTSSSQVHSPRSKM

Target ORF information:

RefSeq Version	XM_017316772.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus WBP2 N-terminal like (Wbp2nl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017316772.1

ORF Insert Sequence:

ATGGCAGTGA ACCAGAACCA TACAGTGGAC CGCCGTTGGG CCGCCATCCC TCATGGTGAA 
AGGTGCCTGA GGGGACTTTC AGGCTGCTGT CAAGGAGGGA GGAGATGCTC AGGTCTCTTG 
AAGAAATGTT CAGAAGTGGA CCTCTCCTTC CCCCAGTCAC CGCCAGGCTC CAACCTCTTT 
AGTGGTACAA AGAGGGGGGC TCTGTTTCTC ACCTCATACC GGGTGATTTT TGTGACTTCG 
CGTGCAGACA ACGATCCCAT GTTTTCTTTT ACGATGCCAT TTCATCTAAT GAATAACTGC 
ACTGTTGAAC AACCAATCTT TGGTGCAAAC TACATTAAAG GGACAATTCA GGCAGCTCCA 
GACGGTGGCT GGGAAGGTTC TGCTACTTTT AAAATAGTCT TCCGGAAAGG AGGTGCTATC 
GACTTTGCCC AGTTGATGGC CAAAGCTGCC TCTGCGGCTG CCCAAGGAGT TCCACTACGA 
GTTGCAAGCT TCTGGATGGG CCCTCTAGGA ATTTATGTCA TCACTGGGGA CAGGAACATG 
TATGCCCCAC AGGCATACCA AGTTGCCTAT GGAGCCCCAC CTGCGGGATA TGGGGCCTCA 
CCCGTGGGAT ACGGAGTCCC ATCTGCAGGG TATGGAGCCC CACCTGCGGG GTACGGAGCC 
CCGCCAGTGG GTTATGTAGC CCCATCTCCC GGATACGATG TCCTGCCTCC TGGATACGGA 
GCTGTGAGAT ATGGCTCCCC TCCTCCTCTA TATGTAGCTA CCCCAATGGG CTATGGAGTT 
CCACCTCCTG GATATGGACC TCCACCTGTG AGATACGGAT CCCCACCTCC TGGATATGAA 
GCCCCCACTA TGGAGTATGG AGCCCAACCT CCCAGATACG GAACTACACC TATGGGATCC 
GGTTCCCCAC CTCCCAGATA TGAAGCCCCG CCTATGGGGT ATGGAACTCC TCCTTCAGGG 
CGTGAGTCCA TACCTCCTGG ATCAAGGGCC ACGTCTGTAG CACAGGAAGC TCCACCTGCT 
GGGTCTGAAG CAGGCCATCC CATGTCTGTG GCAGTCCAGA ATCCTGAATT CCAGGCATCT 
TTTCCCTCTA CCTCGTCCTC ACAAGTCCAC TCTCCCCGCT CTAAGATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation.
Biology of reproduction93(4)94(2015 Oct)
Satouh Y,Nozawa K,Ikawa M

Sperm-derived WW domain-binding protein, PAWP, elicits calcium oscillations and oocyte activation in humans and mice.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(10)4434-40(2014 Oct)
Aarabi M,Balakier H,Bashar S,Moskovtsev SI,Sutovsky P,Librach CL,Oko R

Sperm-specific post-acrosomal WW-domain binding protein (PAWP) does not cause Ca2+ release in mouse oocytes.
Molecular human reproduction20(10)938-47(2014 Oct)
Nomikos M,Sanders JR,Theodoridou M,Kashir J,Matthews E,Nounesis G,Lai FA,Swann K

Expression analysis of PAWP during mouse embryonic stem cell-based spermatogenesis in vitro.
In vitro cellular & developmental biology. Animal50(5)475-81(2014)
Nourashrafeddin S,Aarabi M,Miryounesi M,Ebrahimzadeh-Vesal R,Zarghami N,Modarressi MH,Nouri M

The postacrosomal assembly of sperm head protein, PAWP, is independent of acrosome formation and dependent on microtubular manchette transport.
Developmental biology312(2)471-83(2007 Dec)
Wu AT,Sutovsky P,Xu W,van der Spoel AC,Platt FM,Oko R

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Wbp2nl cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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