Ccdc7a cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ccdc7a
Entrez Gene ID	74703
Full Name	coiled-coil domain containing 7A
Synonyms	4930517G15Rik,4930540C21Rik,Biot2,Ccdc7
General protein information	Preferred Names coiled-coil domain containing 7A Names coiled-coil domain-containing protein 7 coiled-coil domain containing 7 putative cancer/testis antigen testis-specific antigen
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E2

mRNA and Protein(s)

mRNA	Protein	Name
NM_029061.3	NP_083337.1	coiled-coil domain-containing protein 7 isoform 1
NM_001197041.1	NP_001183970.1	coiled-coil domain-containing protein 7 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	LOC498971	NP_001041401.1
Pan troglodytes (chimpanzee)	CCDC7	XP_003312563.1
Mus musculus (house mouse)	Ccdc7	NP_083337.1

Gene Ontology
Bibliography

Related articles in PubMed

Deficiency of Prdm13, a dorsomedial hypothalamus-enriched gene, mimics age-associated changes in sleep quality and adiposity.
Satoh A, Brace CS, Rensing N, Imai S
Aging cell14(2)209-18(2015 Apr)

RNA interference of Biot2 induces G1 phase arrest and apoptosis in mouse colorectal cancer cell line.
Zhou C, Zhang P, Xu GC, Wu DM, Liu RY, Zeng Q, Wang CT
Oncology research22(2)93-103(2015)

Sirt1 extends life span and delays aging in mice through the regulation of Nk2 homeobox 1 in the DMH and LH.
Satoh A, Brace CS, Rensing N, Cliften P, Wozniak DF, Herzog ED, Yamada KA, Imai S
Cell metabolism18(3)416-30(2013 Sep)

SIRT1 promotes the central adaptive response to diet restriction through activation of the dorsomedial and lateral nuclei of the hypothalamus.
Satoh A, Brace CS, Ben-Josef G, West T, Wozniak DF, Holtzman DM, Herzog ED, Imai S
The Journal of neuroscience : the official journal of the Society for Neuroscience30(30)10220-32(2010 Jul)

[Analysis of expression pattern of a novel testis-highly expressed gene Biot2-L and the primary study on its role in testis development].
Wang H, Zhang P, Wang CT
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition40(5)853-6(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

silencing Biot2 in CT26 cells by RNA interference can inhibit cell growth in vitro and in vivo.
Title: RNA interference of Biot2 induces G1 phase arrest and apoptosis in mouse colorectal cancer cell line.

Biot2-L was highly expressed in adult testis, and its expression increased regularly during testis development.
Title: [Analysis of expression pattern of a novel testis-highly expressed gene Biot2-L and the primary study on its role in testis development].

The Biot2 transcript was detected only and strongly in the testis tissues and abundantly in five types of murine cancer cell line.
Title: RNA interference against Biot2, a novel mouse testis-specific gene, inhibits the growth of tumor cells.

The following Ccdc7a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ccdc7a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu17554	NM_029061.3 Latest version!	Mus musculus coiled-coil domain containing 7A (Ccdc7a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu17606	NM_001197041.1 Latest version!	Mus musculus coiled-coil domain containing 7A (Ccdc7a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ccdc7a ( NM_029061.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_083337.1 Mus musculus coiled-coil domain containing 7A (Ccdc7a), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu17554
Clone ID Related Accession (Same CDS sequence)	NM_029061.3
Accession Version	NM_029061.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1116bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	coiled-coil domain-containing protein 7 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK016006.1 and AK015811.1. On Oct 2, 2010 this sequence version replaced NM_029061.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AY854152.1, AK015811.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATGTG CAAAGCATCC ATCAACCATT AGTATGAAAT TGACAAGTGT TCCAGAATTA 
CCTTATAAGA AAGGCTTACT GAATTCATCA CCTAAGCCAA AAGAAAAACA TAATGCCAAG 
TCAAAGTATG GTAAAAATGA ATCAATGGTC CTGAGGTCGC CACCCACAGG AGAGTCAATT 
GTACGCTTTG CCTTGCCAAT ACCATTGAGT AAAACGAAGG ACAAAATATC AGCTGATGAA 
ATGGTCAGGA GGATTACCAC AAATCTGAAG ATGGTTGTTT CTAACTTGGA AGACACCTAT 
GGAGCTTGCT ATGATAATGG AGAAAAAGCA GCAGAAAAAA GTGAAGCAGA AGGCTTGTCT 
ATTGGTGATG ATGTGAGTTC GTTCTTGTTG TGCTGTTCAC AATTTACATC TCAGCTGGAA 
GAAGCAGTTA AGGAAGAATG TGGCGCTTTG GAGTCTCTTT ACAAGTGGTT TCAGCAACAA 
GTCAACCAAA TGGAGGAGAT TAGCAAGGAC CAAAGTAATT TAGAAGAACT TCAATCAGAT 
GGTAAAACGG CCAGTTTGAA CATTGTACAA ATTGCAAAAC TGGCACGTAA ATTCGAAGAT 
TTTAAAAGTC GCCTAAAAAC GCGCAAGGAG GTCATGCAGA CTAAAAATGA GGATAAAGAG 
ATAATGGCAG AAACATTGAA ACATTATGGA CTTATGGAAA AGCAGATTGA AGAGTTCATA 
ACATCCCATT CAGCTTTAGA ATCTCAAACT GAAACTGAAA GTCAGTCAGG AACTCCTTCA 
GTGACTACTC GGATGGCCAG GATGATAAAA ATATTTGAAA ATCAGTCAAC TATGTTAGAG 
AAAGCTTTAA ATGATCAGCA AACAATTGAG TCTAAATATA AACAGTTGGA AACTGATTTT 
CAAATGTTAA TAATGGAGAA AACCCTGCTG GAAGCAGAAA TACGAAGGCT GAGAGAAATA 
GAGAGAGTAA AGTCTGCAGC CAAAGAAGAG CAAACCAAGA AATCTGGAAA ATCAGAAAAG 
AAAAAATTTA AAGAGAAGGA AAAGTACGAC ATGAAGATAT GTTATCTTTT TATTCCTCAA 
CTTGTAAAAA TGTACCTTAA TCACGGTTTA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_083337.1
CDS	395..1510
Translation	MKCAKHPSTISMKLTSVPELPYKKGLLNSSPKPKEKHNAKSKYGKNESMVLRSPPTGESIVRFALPIPLSKTKDKISADEMVRRITTNLKMVVSNLEDTYGACYDNGEKAAEKSEAEGLSIGDDVSSFLLCCSQFTSQLEEAVKEECGALESLYKWFQQQVNQMEEISKDQSNLEELQSDGKTASLNIVQIAKLARKFEDFKSRLKTRKEVMQTKNEDKEIMAETLKHYGLMEKQIEEFITSHSALESQTETESQSGTPSVTTRMARMIKIFENQSTMLEKALNDQQTIESKYKQLETDFQMLIMEKTLLEAEIRRLREIERVKSAAKEEQTKKSGKSEKKKFKEKEKYDMKICYLFIPQLVKMYLNHGLF

Target ORF information:

RefSeq Version	NM_029061.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil domain containing 7A (Ccdc7a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_029061.3

ORF Insert Sequence:

ATGAAATGTG CAAAGCATCC ATCAACCATT AGTATGAAAT TGACAAGTGT TCCAGAATTA 
CCTTATAAGA AAGGCTTACT GAATTCATCA CCTAAGCCAA AAGAAAAACA TAATGCCAAG 
TCAAAGTATG GTAAAAATGA ATCAATGGTC CTGAGGTCGC CACCCACAGG AGAGTCAATT 
GTACGCTTTG CCTTGCCAAT ACCATTGAGT AAAACGAAGG ACAAAATATC AGCTGATGAA 
ATGGTCAGGA GGATTACCAC AAATCTGAAG ATGGTTGTTT CTAACTTGGA AGACACCTAT 
GGAGCTTGCT ATGATAATGG AGAAAAAGCA GCAGAAAAAA GTGAAGCAGA AGGCTTGTCT 
ATTGGTGATG ATGTGAGTTC GTTCTTGTTG TGCTGTTCAC AATTTACATC TCAGCTGGAA 
GAAGCAGTTA AGGAAGAATG TGGCGCTTTG GAGTCTCTTT ACAAGTGGTT TCAGCAACAA 
GTCAACCAAA TGGAGGAGAT TAGCAAGGAC CAAAGTAATT TAGAAGAACT TCAATCAGAT 
GGTAAAACGG CCAGTTTGAA CATTGTACAA ATTGCAAAAC TGGCACGTAA ATTCGAAGAT 
TTTAAAAGTC GCCTAAAAAC GCGCAAGGAG GTCATGCAGA CTAAAAATGA GGATAAAGAG 
ATAATGGCAG AAACATTGAA ACATTATGGA CTTATGGAAA AGCAGATTGA AGAGTTCATA 
ACATCCCATT CAGCTTTAGA ATCTCAAACT GAAACTGAAA GTCAGTCAGG AACTCCTTCA 
GTGACTACTC GGATGGCCAG GATGATAAAA ATATTTGAAA ATCAGTCAAC TATGTTAGAG 
AAAGCTTTAA ATGATCAGCA AACAATTGAG TCTAAATATA AACAGTTGGA AACTGATTTT 
CAAATGTTAA TAATGGAGAA AACCCTGCTG GAAGCAGAAA TACGAAGGCT GAGAGAAATA 
GAGAGAGTAA AGTCTGCAGC CAAAGAAGAG CAAACCAAGA AATCTGGAAA ATCAGAAAAG 
AAAAAATTTA AAGAGAAGGA AAAGTACGAC ATGAAGATAT GTTATCTTTT TATTCCTCAA 
CTTGTAAAAA TGTACCTTAA TCACGGTTTA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ccdc7a ( NM_001197041.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001183970.1 Mus musculus coiled-coil domain containing 7A (Ccdc7a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu17606
Clone ID Related Accession (Same CDS sequence)	NM_001197041.1
Accession Version	NM_001197041.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	coiled-coil domain-containing protein 7 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK016006.1 and AK015811.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK016006.1, EF100607.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAATGTG CAAAGCATCC ATCAACCATT AGTATGAAAT TGACAAGTGT TCCAGAATTA 
CCTTATAAGA AAGGCTTACT GAATTCATCA CCTAAGCCAA AAGAAAAACA TAATGCCAAG 
TCAAAGTATG GTAAAAATGA ATCAATGGTC CTGAGGTCGC CACCCACAGG AGAGTCAATT 
GTACGCTTTG CCTTGCCAAT ACCATTGAGT AAAACGAAGG ACAAAATATC AGCTGATGAA 
ATGGTCAGGA GGATTACCAC AAATCTGAAG ATGGTTGTTT CTAACTTGGA AGACACCTAT 
GGAGCTTGCT ATGATAATGG AGAAAAAGCA GCAGAAAAAA GTGAAGCAGA AGGCTTGTCT 
ATTGGTGATG ATGTGAGTTC GTTCTTGTTG TGCTGTTCAC AATTTACATC TCAGCTGGAA 
GAAGCAGTTA AGGAAGAATG TGGCAAAAAA ACATTTCAAA ACTATTGCCA AAATTTTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001183970.1
CDS	395..877
Translation	MKCAKHPSTISMKLTSVPELPYKKGLLNSSPKPKEKHNAKSKYGKNESMVLRSPPTGESIVRFALPIPLSKTKDKISADEMVRRITTNLKMVVSNLEDTYGACYDNGEKAAEKSEAEGLSIGDDVSSFLLCCSQFTSQLEEAVKEECGKKTFQNYCQNFQ

Target ORF information:

RefSeq Version	NM_001197041.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus coiled-coil domain containing 7A (Ccdc7a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001197041.1

ORF Insert Sequence:

ATGAAATGTG CAAAGCATCC ATCAACCATT AGTATGAAAT TGACAAGTGT TCCAGAATTA 
CCTTATAAGA AAGGCTTACT GAATTCATCA CCTAAGCCAA AAGAAAAACA TAATGCCAAG 
TCAAAGTATG GTAAAAATGA ATCAATGGTC CTGAGGTCGC CACCCACAGG AGAGTCAATT 
GTACGCTTTG CCTTGCCAAT ACCATTGAGT AAAACGAAGG ACAAAATATC AGCTGATGAA 
ATGGTCAGGA GGATTACCAC AAATCTGAAG ATGGTTGTTT CTAACTTGGA AGACACCTAT 
GGAGCTTGCT ATGATAATGG AGAAAAAGCA GCAGAAAAAA GTGAAGCAGA AGGCTTGTCT 
ATTGGTGATG ATGTGAGTTC GTTCTTGTTG TGCTGTTCAC AATTTACATC TCAGCTGGAA 
GAAGCAGTTA AGGAAGAATG TGGCAAAAAA ACATTTCAAA ACTATTGCCA AAATTTTCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deficiency of Prdm13, a dorsomedial hypothalamus-enriched gene, mimics age-associated changes in sleep quality and adiposity.
Aging cell14(2)209-18(2015 Apr)
Satoh A,Brace CS,Rensing N,Imai S

RNA interference of Biot2 induces G1 phase arrest and apoptosis in mouse colorectal cancer cell line.
Oncology research22(2)93-103(2015)
Zhou C,Zhang P,Xu GC,Wu DM,Liu RY,Zeng Q,Wang CT

Sirt1 extends life span and delays aging in mice through the regulation of Nk2 homeobox 1 in the DMH and LH.
Cell metabolism18(3)416-30(2013 Sep)
Satoh A,Brace CS,Rensing N,Cliften P,Wozniak DF,Herzog ED,Yamada KA,Imai S

SIRT1 promotes the central adaptive response to diet restriction through activation of the dorsomedial and lateral nuclei of the hypothalamus.
The Journal of neuroscience : the official journal of the Society for Neuroscience30(30)10220-32(2010 Jul)
Satoh A,Brace CS,Ben-Josef G,West T,Wozniak DF,Holtzman DM,Herzog ED,Imai S

[Analysis of expression pattern of a novel testis-highly expressed gene Biot2-L and the primary study on its role in testis development].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition40(5)853-6(2009 Sep)
Wang H,Zhang P,Wang CT