Cers5 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cers5
Entrez Gene ID	71949
Full Name	ceramide synthase 5
Synonyms	2310081H14Rik,AU045339,AW544927,Lass5,Trh4
General protein information	Preferred Names ceramide synthase 5 Names ceramide synthase 5 LAG1 homolog, ceramide synthase 5 LAG1 longevity assurance homolog 5 TRAM homolog 4 longevity assurance homolog 5 translocating chain-associating membrane protein homolog 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_028015.2	NP_082291.1	ceramide synthase 5

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CERS5	XP_005636909.1
Mus musculus (house mouse)	Cers5	NP_082291.1
Pan troglodytes (chimpanzee)	LASS5	XP_003313752.1
Oryza sativa (rice)	Os03g0264000	NP_001049639.1
Bos taurus (cattle)	CERS5	NP_001095602.1
Rattus norvegicus (Norway rat)	Cers5	NP_001102463.1
Gallus gallus (chicken)	CERS5	XP_004949782.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001761	XP_321321.5
Arabidopsis thaliana (thale cress)	LOH2	NP_188557.1
Danio rerio (zebrafish)	cers5	NP_955922.1
Caenorhabditis elegans (roundworm)	hyl-1	NP_501459.1
Xenopus tropicalis (tropical clawed frog)	cers5	XP_002935791.2
Homo sapiens (human)	CERS5	NP_671723.1
	LASS5	XP_001102810.2
Drosophila melanogaster (fruit fly)	schlank	NP_652526.1
Caenorhabditis elegans (roundworm)	hyl-2	NP_508803.3

Gene Ontology
Bibliography

Related articles in PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
Sundberg JP, Dadras SS, Silva KA, Kennedy VE, Garland G, Murray SA, Sundberg BA, Schofield PN, Pratt CH
PloS one12(7)e0180682(2017)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Enzyme Activities of the Ceramide Synthases CERS2-6 Are Regulated by Phosphorylation in the C-terminal Region.
Sassa T, Hirayama T, Kihara A
The Journal of biological chemistry291(14)7477-87(2016 Apr)

Ceramide Synthase 5 Is Essential to Maintain C16:0-Ceramide Pools and Contributes to the Development of Diet-induced Obesity.
Gosejacob D, J?ger PS, Vom Dorp K, Frejno M, Carstensen AC, K?hnke M, Degen J, D?rmann P, Hoch M
The Journal of biological chemistry291(13)6989-7003(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that the phosphorylation of ceramide synthases may be a key regulatory point in the control of the distribution and levels of sphingolipids of various acyl-chain lengths.
Title: Enzyme Activities of the Ceramide Synthases CERS2-6 Are Regulated by Phosphorylation in the C-terminal Region.

Ceramide synthase 5 is essential to maintain C16:0-ceramide pools and contributes to the development of diet-induced obesity.
Title: Ceramide Synthase 5 Is Essential to Maintain C16:0-Ceramide Pools and Contributes to the Development of Diet-induced Obesity.

Transfection of Lass5 cDNA into or pulse of Lass5 peptide onto B7-1 positive RMA-S cells overcomes the requirement of the B7-1/CD28 signal for T effector response.
Title: Limited density of an antigen presented by RMA-S cells requires B7-1/CD28 signaling to enhance T-cell immunity at the effector phase.

Data indicate that oxidized phospholipids (OxPLs)-induced ceramide synthases (CerS1-Cers6) activity in macrophages is responsible for the accumulation of ceramide.
Title: Oxidized phospholipids induce ceramide accumulation in RAW 264.7 macrophages: role of ceramide synthases.

Ceramide synthase 5 mediates lipid-induced autophagy and hypertrophy in cardiomyocytes.
Title: Ceramide synthase 5 mediates lipid-induced autophagy and hypertrophy in cardiomyocytes.

The following Cers5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cers5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18680	NM_028015.2 Latest version!	Mus musculus ceramide synthase 5 (Cers5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cers5 ( NM_028015.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082291.1 Mus musculus ceramide synthase 5 (Cers5), mRNA.

Price & Availability↑

CloneID	OMu18680
Clone ID Related Accession (Same CDS sequence)	NM_028015.2
Accession Version	NM_028015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	ceramide synthase 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC046797.1. On Jun 19, 2003 this sequence version replaced NM_028015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046797.1, AK010241.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCGACTG CAGCAGCGGA AACCCTGGGC CTCCTGTGGG GTTGGCTGTG GAGCGAGAGC 
TTCTGGCTGC CTCAGAACGT GAGCTGGGCG GACCTGGAGG GCCCGGGCGA CGGCTACGGC 
TACCCGCGCG CCCAGCACGT GCTGTCGGTG TTCCCGCTGG CAGTGTGCAT CTTCTCCGTG 
AGGATGCTGT TTGAGCGATT TATTGCCAAG CCCTGTGCAC TCCGTGTTGG CATTAAAGAC 
AGTCCTGTCA ATAAGGTGGA ACCCAATGAC ACCCTTGAGA AGGTGTTCGT ATCTGTTACT 
AAGTATCCTG ATGAGAAAAG GTTGAAGGGC CTATCAAAGC AACTGGACTG GAGTGTTCGA 
AAAATCCAAT GCTGGTTTCG CCATCGGAGG AATCAGGACA AGCCTCCAAC GCTCACGAAA 
TTCTGTGAAA GCATGTGGAG ATTCACTTAT TATTTATGTA TCTTCTGCTA CGGAATCAGA 
TTTCTCTGGT CGATGCCTTG GTTCTGGGAT ACCCGACAGT GCTGGTACAA CTATCCGTAT 
CAGCCACTCT CGAGAGAGCT GTATTACTAT TACATCACAC AACTGGCTTT CTACTGGTCT 
CTTATGTTTT CTCAGTTTAT AGATGTTAAA AGAAAGGACT TCCTGATGAT GTTTATTCAT 
CATATGATTG GCATTATGCT CACCACTTTC TCCTACGTCA ACAACATGGT GCGAGTGGGA 
GCACTCATCT TCTGTCTGCA TGACTTCGCA GACCCCTTAC TGGAAGCTGC CAAGATGGCC 
AATTATGCCA GACGTGAGCG GCTCTGTACC ACCCTTTTTG TGATCTTTGG CGCAGCTTTT 
ATAGTTTCTC GTCTAGCAAT CTTCCCATTG TGGATTCTGA ACACAACCCT CTTTGAAAGC 
TGGGAGATTA TTGGGCCCTA CCCTTCCTGG TGGCTCTTCA ATGCCCTTCT CCTGATCCTG 
CAGGTGCTCC ATGCCATCTG GTCCTACCTA ATTGTCCAGA CTGCTTCCAA AGCCTTGAGC 
CGGGGAAAGG TGTCTAAGGA TGACCGCAGT GATGTAGAGA GCAGCTCAGA GGAAGAAGAT 
GAGACAACCC ACAAAAACAA CCTCTCTGGC AGCAGCTCTA GCAATGGTGC CAACTGCATG 
AACGGCTACA TGGGAGGCAG CCACTTGGCT GAAGAGCAGG GGACTTGTAA AGCCACTGGC 
AACCTACATT TTAGGGCCTC CCCACATCTA CACTCCTGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082291.1
CDS	137..1381
Translation	MATAAAETLGLLWGWLWSESFWLPQNVSWADLEGPGDGYGYPRAQHVLSVFPLAVCIFSVRMLFERFIAKPCALRVGIKDSPVNKVEPNDTLEKVFVSVTKYPDEKRLKGLSKQLDWSVRKIQCWFRHRRNQDKPPTLTKFCESMWRFTYYLCIFCYGIRFLWSMPWFWDTRQCWYNYPYQPLSRELYYYYITQLAFYWSLMFSQFIDVKRKDFLMMFIHHMIGIMLTTFSYVNNMVRVGALIFCLHDFADPLLEAAKMANYARRERLCTTLFVIFGAAFIVSRLAIFPLWILNTTLFESWEIIGPYPSWWLFNALLLILQVLHAIWSYLIVQTASKALSRGKVSKDDRSDVESSSEEEDETTHKNNLSGSSSSNGANCMNGYMGGSHLAEEQGTCKATGNLHFRASPHLHSCD

Target ORF information:

RefSeq Version	NM_028015.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ceramide synthase 5 (Cers5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028015.2

ORF Insert Sequence:

ATGGCGACTG CAGCAGCGGA AACCCTGGGC CTCCTGTGGG GTTGGCTGTG GAGCGAGAGC 
TTCTGGCTGC CTCAGAACGT GAGCTGGGCG GACCTGGAGG GCCCGGGCGA CGGCTACGGC 
TACCCGCGCG CCCAGCACGT GCTGTCGGTG TTCCCGCTGG CAGTGTGCAT CTTCTCCGTG 
AGGATGCTGT TTGAGCGATT TATTGCCAAG CCCTGTGCAC TCCGTGTTGG CATTAAAGAC 
AGTCCTGTCA ATAAGGTGGA ACCCAATGAC ACCCTTGAGA AGGTGTTCGT ATCTGTTACT 
AAGTATCCTG ATGAGAAAAG GTTGAAGGGC CTATCAAAGC AACTGGACTG GAGTGTTCGA 
AAAATCCAAT GCTGGTTTCG CCATCGGAGG AATCAGGACA AGCCTCCAAC GCTCACGAAA 
TTCTGTGAAA GCATGTGGAG ATTCACTTAT TATTTATGTA TCTTCTGCTA CGGAATCAGA 
TTTCTCTGGT CGATGCCTTG GTTCTGGGAT ACCCGACAGT GCTGGTACAA CTATCCGTAT 
CAGCCACTCT CGAGAGAGCT GTATTACTAT TACATCACAC AACTGGCTTT CTACTGGTCT 
CTTATGTTTT CTCAGTTTAT AGATGTTAAA AGAAAGGACT TCCTGATGAT GTTTATTCAT 
CATATGATTG GCATTATGCT CACCACTTTC TCCTACGTCA ACAACATGGT GCGAGTGGGA 
GCACTCATCT TCTGTCTGCA TGACTTCGCA GACCCCTTAC TGGAAGCTGC CAAGATGGCC 
AATTATGCCA GACGTGAGCG GCTCTGTACC ACCCTTTTTG TGATCTTTGG CGCAGCTTTT 
ATAGTTTCTC GTCTAGCAAT CTTCCCATTG TGGATTCTGA ACACAACCCT CTTTGAAAGC 
TGGGAGATTA TTGGGCCCTA CCCTTCCTGG TGGCTCTTCA ATGCCCTTCT CCTGATCCTG 
CAGGTGCTCC ATGCCATCTG GTCCTACCTA ATTGTCCAGA CTGCTTCCAA AGCCTTGAGC 
CGGGGAAAGG TGTCTAAGGA TGACCGCAGT GATGTAGAGA GCAGCTCAGA GGAAGAAGAT 
GAGACAACCC ACAAAAACAA CCTCTCTGGC AGCAGCTCTA GCAATGGTGC CAACTGCATG 
AACGGCTACA TGGGAGGCAG CCACTTGGCT GAAGAGCAGG GGACTTGTAA AGCCACTGGC 
AACCTACATT TTAGGGCCTC CCCACATCTA CACTCCTGTG ACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic screening for skin, hair, and nail abnormalities in a large-scale knockout mouse program.
PloS one12(7)e0180682(2017)
Sundberg JP,Dadras SS,Silva KA,Kennedy VE,Garland G,Murray SA,Sundberg BA,Schofield PN,Pratt CH

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Enzyme Activities of the Ceramide Synthases CERS2-6 Are Regulated by Phosphorylation in the C-terminal Region.
The Journal of biological chemistry291(14)7477-87(2016 Apr)
Sassa T,Hirayama T,Kihara A

Ceramide Synthase 5 Is Essential to Maintain C16:0-Ceramide Pools and Contributes to the Development of Diet-induced Obesity.
The Journal of biological chemistry291(13)6989-7003(2016 Mar)
Gosejacob D,J?ger PS,Vom Dorp K,Frejno M,Carstensen AC,K?hnke M,Degen J,D?rmann P,Hoch M