Gtf2a1l cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gtf2a1l
Entrez Gene ID	71828
Full Name	general transcription factor IIA, 1-like
Synonyms	1700011N16Rik,Alf,Gtf2a1lf
General protein information	Preferred Names general transcription factor IIA, 1-like Names TFIIA-alpha and beta-like factor TfIIa-alpha/beta-like factor general transcription factor II A, 1-like factor general transcription factor IIA, 1-like factor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17\|17 E4

mRNA and Protein(s)

mRNA	Protein	Name
NM_023630.2	NP_076119.2	TFIIA-alpha and beta-like factor
XM_011246628.2	XP_011244930.1	TFIIA-alpha and beta-like factor isoform X1
XM_011246628.3	XP_011244930.1	TFIIA-alpha and beta-like factor isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	gtf2a1l	NP_001070039.1
Xenopus tropicalis (tropical clawed frog)	gtf2a1l	NP_001039213.1
Rattus norvegicus (Norway rat)	Gtf2a1l	NP_001012136.1
Canis lupus familiaris (dog)	GTF2A1L	NP_001191772.1
Mus musculus (house mouse)	Gtf2a1l	NP_076119.2
Gallus gallus (chicken)	GTF2A1L	NP_001191795.1
Homo sapiens (human)	GTF2A1L	NP_006863.2
Pan troglodytes (chimpanzee)	GTF2A1L	NP_001191755.1

Gene Ontology
Bibliography

Related articles in PubMed

Rfx2 is required for spermatogenesis in the mouse.
Shawlot W, Vazquez-Chantada M, Wallingford JB, Finnell RH
Genesis (New York, N.Y. : 2000)(2015 Aug)

Cleavage of TFIIA by Taspase1 activates TRF2-specified mammalian male germ cell programs.
Oyama T, Sasagawa S, Takeda S, Hess RA, Lieberman PM, Cheng EH, Hsieh JJ
Developmental cell27(2)188-200(2013 Oct)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

Developmental and cell type-specific regulation of core promoter transcription factors in germ cells of frogs and mice.
Xiao L, Kim M, DeJong J
Gene expression patterns : GEP6(4)409-19(2006 Apr)

Proteolytic cleavage of ALF into alpha- and beta-subunits that form homologous and heterologous complexes with somatic TFIIA and TRF2 in male germ cells.
Catena R, Argentini M, Martianov I, Parello C, Brancorsini S, Parvinen M, Sassone-Corsi P, Davidson I
FEBS letters579(16)3401-10(2005 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taspase1 cleaves a ubiquitously expressed GTF (TFIIA) to enable tissue-specific (testis) transcription, meeting the demand for sophisticated regulation of distinct subsets of genes in higher organisms.
Title: Cleavage of TFIIA by Taspase1 activates TRF2-specified mammalian male germ cell programs.

Endogenous ALF is proteolytically cleaved to give alpha- and beta-subunits and its cleavage site was mapped.
Title: Proteolytic cleavage of ALF into alpha- and beta-subunits that form homologous and heterologous complexes with somatic TFIIA and TRF2 in male germ cells.

unique core promoter that is active in both male and female reproductive tissue suggesting ALF may have a regulatory role in male and female gametogenic gene expression programs.
Title: A short core promoter drives expression of the ALF transcription factor in reproductive tissues of male and female mice.

a role for ALF in the assembly and stabilization of initiation complexes in germ cells is likely to be similar or identical to the role of TFIIA in somatic cells.
Title: The germ cell-specific transcription factor ALF. Structural properties and stabilization of the TATA-binding protein (TBP)-DNA complex.

The following Gtf2a1l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gtf2a1l cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18967	NM_023630.2 Latest version!	Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu70763	XM_011246628.3 Latest version!	Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu70763	XM_011246628.2	Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gtf2a1l ( NM_023630.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076119.2 Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), mRNA.

Price & Availability↑

CloneID	OMu18967
Clone ID Related Accession (Same CDS sequence)	NM_023630.2
Accession Version	NM_023630.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	TFIIA-alpha and beta-like factor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY092414.1 and AK005881.1. On Jul 30, 2009 this sequence version replaced NM_023630.1. ##Evidence-Data-START## Transcript exon combination :: AK005881.1, BC050756.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849384, SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTCA TCAACCTGGT GCCCAAACTC TACCAGTCTG TAATTGAAGA TGTCATCGAG 
GGCGTGCGGG ACCTGTTTGC TGAGGAAGGC ATCGAGGAGC AGGTGTTGAA AGACCTGAAG 
AAGCTCTGGG AAACCAAAGT GTTACAATCT AAAGCCACAG AGGACTTCTT CAGAAACAGC 
ACGCAGGTGC CTCTTCTCAC TCTCCAGCTG CCTCATGCCT TACCACCAGC CCTGCAGCCC 
GAAGCATCGC TGCTGATCCC AGCTGGTAGA ACTCTGCCGA GTTTTACGCC GGAAGACCTG 
AACACCGCCA ACTGTGGTGC AAACTTTGCC TTTGCTGGCT ATCCGATCCA CGTCCCAGCA 
GGCATGGCCT TCCAGACGGC ATCTGGTCAC CTTTACAAAG TCAATGTACC AGTCATGGTG 
ACACAGACTT CTGGGAGAAC AGAAATTCTC CAGCATCCAT TTCAGCAAGT CCTTCAGCAG 
CTCGGGCAGC CTTTAGTAAT ACAGACCACT GTTCCAACAT TGCACCCATG TTCTCTTCAA 
GCAGCAACTG AGAAATCCCT CAGAATGGAA GCTGTGCTGC AGCCACCTCC CATTCTGCAT 
CCTCCTCCAG TGGACAGGAC ACATGTAGAA AATGCTGCGA GCGACAGGCG CCTTCTCCCG 
GGAAATGAGC TGAGGCCGCA GGAAAGCTCT CCATACCTCA GCCTTCCCGG TGTGGGCTTT 
CCTCCTCAGG CCGCTCTGAC AGAGTCTAGC CTGGAGCCAG TGCTTGGTGT CTCAGCGAGC 
CTGACTCAGA ATCTGCACAG TGACCCTTTC TCACAGGGCC CCCCAGGCCC TCTCCACCAC 
CACTTGCTAG AGTCACAGCT TCAAAGCCTT AAAGACAGTA TATATGGATG CGACTCCACA 
AAGCAACTGA GAAAAGCAGA GGAGCCCAGC AGCCTCCGTG TGTCAGAGAA GAATTGTACT 
TCAGAGAGGG ATCTGAATAT TCGGGTAACC GATGATGATA TTAATGAAAT AATCCAAATA 
GATGGAACCG GCGATAACTC TTCTACTGAA GAGATGGGAA GTATAAGGGA TGCAGATGAG 
AATGAATTCC CAGGGATCAT TGATGCCGGC GACCTCAATG TGCTTGAAGA AGTGGACAGC 
GTATCGAATG AAGATTCAAC TGCAAATAGC AGCGACAACG AGGACCATCA AATAAATGCC 
CCAGAAGAGG ATCCCCTAAA TTCTGGCGAT GATGTCAGTG AGCAGGATGT GCCAGACCTG 
TTTGATACAG AGAATGTAAT TGTCTGTCAG TATGATAAGA TCCACCGGAG CAAGAACAGA 
TGGAAATTCT ACTTGAAAGA TGGTGTCATG TGCTTTGGAG GGAGAGACTA CGTATTTGCC 
AAAGCCATTG GTGAAGCTGA GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076119.2
CDS	52..1458
Translation	MAFINLVPKLYQSVIEDVIEGVRDLFAEEGIEEQVLKDLKKLWETKVLQSKATEDFFRNSTQVPLLTLQLPHALPPALQPEASLLIPAGRTLPSFTPEDLNTANCGANFAFAGYPIHVPAGMAFQTASGHLYKVNVPVMVTQTSGRTEILQHPFQQVLQQLGQPLVIQTTVPTLHPCSLQAATEKSLRMEAVLQPPPILHPPPVDRTHVENAASDRRLLPGNELRPQESSPYLSLPGVGFPPQAALTESSLEPVLGVSASLTQNLHSDPFSQGPPGPLHHHLLESQLQSLKDSIYGCDSTKQLRKAEEPSSLRVSEKNCTSERDLNIRVTDDDINEIIQIDGTGDNSSTEEMGSIRDADENEFPGIIDAGDLNVLEEVDSVSNEDSTANSSDNEDHQINAPEEDPLNSGDDVSEQDVPDLFDTENVIVCQYDKIHRSKNRWKFYLKDGVMCFGGRDYVFAKAIGEAEW

Target ORF information:

RefSeq Version	NM_023630.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023630.2

ORF Insert Sequence:

ATGGCCTTCA TCAACCTGGT GCCCAAACTC TACCAGTCTG TAATTGAAGA TGTCATCGAG 
GGCGTGCGGG ACCTGTTTGC TGAGGAAGGC ATCGAGGAGC AGGTGTTGAA AGACCTGAAG 
AAGCTCTGGG AAACCAAAGT GTTACAATCT AAAGCCACAG AGGACTTCTT CAGAAACAGC 
ACGCAGGTGC CTCTTCTCAC TCTCCAGCTG CCTCATGCCT TACCACCAGC CCTGCAGCCC 
GAAGCATCGC TGCTGATCCC AGCTGGTAGA ACTCTGCCGA GTTTTACGCC GGAAGACCTG 
AACACCGCCA ACTGTGGTGC AAACTTTGCC TTTGCTGGCT ATCCGATCCA CGTCCCAGCA 
GGCATGGCCT TCCAGACGGC ATCTGGTCAC CTTTACAAAG TCAATGTACC AGTCATGGTG 
ACACAGACTT CTGGGAGAAC AGAAATTCTC CAGCATCCAT TTCAGCAAGT CCTTCAGCAG 
CTCGGGCAGC CTTTAGTAAT ACAGACCACT GTTCCAACAT TGCACCCATG TTCTCTTCAA 
GCAGCAACTG AGAAATCCCT CAGAATGGAA GCTGTGCTGC AGCCACCTCC CATTCTGCAT 
CCTCCTCCAG TGGACAGGAC ACATGTAGAA AATGCTGCGA GCGACAGGCG CCTTCTCCCG 
GGAAATGAGC TGAGGCCGCA GGAAAGCTCT CCATACCTCA GCCTTCCCGG TGTGGGCTTT 
CCTCCTCAGG CCGCTCTGAC AGAGTCTAGC CTGGAGCCAG TGCTTGGTGT CTCAGCGAGC 
CTGACTCAGA ATCTGCACAG TGACCCTTTC TCACAGGGCC CCCCAGGCCC TCTCCACCAC 
CACTTGCTAG AGTCACAGCT TCAAAGCCTT AAAGACAGTA TATATGGATG CGACTCCACA 
AAGCAACTGA GAAAAGCAGA GGAGCCCAGC AGCCTCCGTG TGTCAGAGAA GAATTGTACT 
TCAGAGAGGG ATCTGAATAT TCGGGTAACC GATGATGATA TTAATGAAAT AATCCAAATA 
GATGGAACCG GCGATAACTC TTCTACTGAA GAGATGGGAA GTATAAGGGA TGCAGATGAG 
AATGAATTCC CAGGGATCAT TGATGCCGGC GACCTCAATG TGCTTGAAGA AGTGGACAGC 
GTATCGAATG AAGATTCAAC TGCAAATAGC AGCGACAACG AGGACCATCA AATAAATGCC 
CCAGAAGAGG ATCCCCTAAA TTCTGGCGAT GATGTCAGTG AGCAGGATGT GCCAGACCTG 
TTTGATACAG AGAATGTAAT TGTCTGTCAG TATGATAAGA TCCACCGGAG CAAGAACAGA 
TGGAAATTCT ACTTGAAAGA TGGTGTCATG TGCTTTGGAG GGAGAGACTA CGTATTTGCC 
AAAGCCATTG GTGAAGCTGA GTGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtf2a1l ( XM_011246628.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244930.1 Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70763
Clone ID Related Accession (Same CDS sequence)	XM_011246628.2 , XM_011246628.3
Accession Version	XM_011246628.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	TFIIA-alpha and beta-like factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011246628.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCC AGACGGCATC TGGTCACCTT TACAAAGTCA ATGTACCAGT CATGGTGACA 
CAGACTTCTG GGAGAACAGA AATTCTCCAG CATCCATTTC AGCAAGTCCT TCAGCAGCTC 
GGGCAGCCTT TAGTAATACA GACCACTGTT CCAACATTGC ACCCATGTTC TCTTCAAGCA 
GCAACTGAGA AATCCCTCAG AATGGAAGCT GTGCTGCAGC CACCTCCCAT TCTGCATCCT 
CCTCCAGTGG ACAGGACACA TGTAGAAAAT GCTGCGAGCG ACAGGCGCCT TCTCCCGGGA 
AATGAGCTGA GGCCGCAGGA AAGCTCTCCA TACCTCAGCC TTCCCGGTGT GGGCTTTCCT 
CCTCAGGCCG CTCTGACAGA GTCTAGCCTG GAGCCAGTGC TTGGTGTCTC AGCGAGCCTG 
ACTCAGAATC TGCACAGTGA CCCTTTCTCA CAGGGCCCCC CAGGCCCTCT CCACCACCAC 
TTGCTAGAGT CACAGCTTCA AAGCCTTAAA GACAGTATAT ATGGATGCGA CTCCACAAAG 
CAACTGAGAA AAGCAGAGGA GCCCAGCAGC CTCCGTGTGT CAGAGAAGAA TTGTACTTCA 
GAGAGGGATC TGAATATTCG GGTAACCGAT GATGATATTA ATGAAATAAT CCAAATAGAT 
GGAACCGGCG ATAACTCTTC TACTGAAGAG ATGGGAAGTA TAAGGGATGC AGATGAGAAT 
GAATTCCCAG GGATCATTGA TGCCGGCGAC CTCAATGTGC TTGAAGAAGT GGACAGCGTA 
TCGAATGAAG ATTCAACTGC AAATAGCAGC GACAACGAGG ACCATCAAAT AAATGCCCCA 
GAAGAGGATC CCCTAAATTC TGGCGATGAT GTCAGTGAGC AGGATGTGCC AGACCTGTTT 
GATACAGAGA ATGTAATTGT CTGTCAGTAT GATAAGATCC ACCGGAGCAA GAACAGATGG 
AAATTCTACT TGAAAGATGG TGTCATGTGC TTTGGAGGGA GAGACTACGT ATTTGCCAAA 
GCCATTGGTG AAGCTGAGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244930.1
CDS	421..1464
Translation	MAFQTASGHLYKVNVPVMVTQTSGRTEILQHPFQQVLQQLGQPLVIQTTVPTLHPCSLQAATEKSLRMEAVLQPPPILHPPPVDRTHVENAASDRRLLPGNELRPQESSPYLSLPGVGFPPQAALTESSLEPVLGVSASLTQNLHSDPFSQGPPGPLHHHLLESQLQSLKDSIYGCDSTKQLRKAEEPSSLRVSEKNCTSERDLNIRVTDDDINEIIQIDGTGDNSSTEEMGSIRDADENEFPGIIDAGDLNVLEEVDSVSNEDSTANSSDNEDHQINAPEEDPLNSGDDVSEQDVPDLFDTENVIVCQYDKIHRSKNRWKFYLKDGVMCFGGRDYVFAKAIGEAEW

Target ORF information:

RefSeq Version	XM_011246628.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246628.2

ORF Insert Sequence:

ATGGCCTTCC AGACGGCATC TGGTCACCTT TACAAAGTCA ATGTACCAGT CATGGTGACA 
CAGACTTCTG GGAGAACAGA AATTCTCCAG CATCCATTTC AGCAAGTCCT TCAGCAGCTC 
GGGCAGCCTT TAGTAATACA GACCACTGTT CCAACATTGC ACCCATGTTC TCTTCAAGCA 
GCAACTGAGA AATCCCTCAG AATGGAAGCT GTGCTGCAGC CACCTCCCAT TCTGCATCCT 
CCTCCAGTGG ACAGGACACA TGTAGAAAAT GCTGCGAGCG ACAGGCGCCT TCTCCCGGGA 
AATGAGCTGA GGCCGCAGGA AAGCTCTCCA TACCTCAGCC TTCCCGGTGT GGGCTTTCCT 
CCTCAGGCCG CTCTGACAGA GTCTAGCCTG GAGCCAGTGC TTGGTGTCTC AGCGAGCCTG 
ACTCAGAATC TGCACAGTGA CCCTTTCTCA CAGGGCCCCC CAGGCCCTCT CCACCACCAC 
TTGCTAGAGT CACAGCTTCA AAGCCTTAAA GACAGTATAT ATGGATGCGA CTCCACAAAG 
CAACTGAGAA AAGCAGAGGA GCCCAGCAGC CTCCGTGTGT CAGAGAAGAA TTGTACTTCA 
GAGAGGGATC TGAATATTCG GGTAACCGAT GATGATATTA ATGAAATAAT CCAAATAGAT 
GGAACCGGCG ATAACTCTTC TACTGAAGAG ATGGGAAGTA TAAGGGATGC AGATGAGAAT 
GAATTCCCAG GGATCATTGA TGCCGGCGAC CTCAATGTGC TTGAAGAAGT GGACAGCGTA 
TCGAATGAAG ATTCAACTGC AAATAGCAGC GACAACGAGG ACCATCAAAT AAATGCCCCA 
GAAGAGGATC CCCTAAATTC TGGCGATGAT GTCAGTGAGC AGGATGTGCC AGACCTGTTT 
GATACAGAGA ATGTAATTGT CTGTCAGTAT GATAAGATCC ACCGGAGCAA GAACAGATGG 
AAATTCTACT TGAAAGATGG TGTCATGTGC TTTGGAGGGA GAGACTACGT ATTTGCCAAA 
GCCATTGGTG AAGCTGAGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gtf2a1l ( XM_011246628.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011244930.1 Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu70763
Clone ID Related Accession (Same CDS sequence)	XM_011246628.2 , XM_011246628.3
Accession Version	XM_011246628.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1044bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	TFIIA-alpha and beta-like factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000083.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011246628.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTCC AGACGGCATC TGGTCACCTT TACAAAGTCA ATGTACCAGT CATGGTGACA 
CAGACTTCTG GGAGAACAGA AATTCTCCAG CATCCATTTC AGCAAGTCCT TCAGCAGCTC 
GGGCAGCCTT TAGTAATACA GACCACTGTT CCAACATTGC ACCCATGTTC TCTTCAAGCA 
GCAACTGAGA AATCCCTCAG AATGGAAGCT GTGCTGCAGC CACCTCCCAT TCTGCATCCT 
CCTCCAGTGG ACAGGACACA TGTAGAAAAT GCTGCGAGCG ACAGGCGCCT TCTCCCGGGA 
AATGAGCTGA GGCCGCAGGA AAGCTCTCCA TACCTCAGCC TTCCCGGTGT GGGCTTTCCT 
CCTCAGGCCG CTCTGACAGA GTCTAGCCTG GAGCCAGTGC TTGGTGTCTC AGCGAGCCTG 
ACTCAGAATC TGCACAGTGA CCCTTTCTCA CAGGGCCCCC CAGGCCCTCT CCACCACCAC 
TTGCTAGAGT CACAGCTTCA AAGCCTTAAA GACAGTATAT ATGGATGCGA CTCCACAAAG 
CAACTGAGAA AAGCAGAGGA GCCCAGCAGC CTCCGTGTGT CAGAGAAGAA TTGTACTTCA 
GAGAGGGATC TGAATATTCG GGTAACCGAT GATGATATTA ATGAAATAAT CCAAATAGAT 
GGAACCGGCG ATAACTCTTC TACTGAAGAG ATGGGAAGTA TAAGGGATGC AGATGAGAAT 
GAATTCCCAG GGATCATTGA TGCCGGCGAC CTCAATGTGC TTGAAGAAGT GGACAGCGTA 
TCGAATGAAG ATTCAACTGC AAATAGCAGC GACAACGAGG ACCATCAAAT AAATGCCCCA 
GAAGAGGATC CCCTAAATTC TGGCGATGAT GTCAGTGAGC AGGATGTGCC AGACCTGTTT 
GATACAGAGA ATGTAATTGT CTGTCAGTAT GATAAGATCC ACCGGAGCAA GAACAGATGG 
AAATTCTACT TGAAAGATGG TGTCATGTGC TTTGGAGGGA GAGACTACGT ATTTGCCAAA 
GCCATTGGTG AAGCTGAGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011244930.1
CDS	422..1465
Translation	MAFQTASGHLYKVNVPVMVTQTSGRTEILQHPFQQVLQQLGQPLVIQTTVPTLHPCSLQAATEKSLRMEAVLQPPPILHPPPVDRTHVENAASDRRLLPGNELRPQESSPYLSLPGVGFPPQAALTESSLEPVLGVSASLTQNLHSDPFSQGPPGPLHHHLLESQLQSLKDSIYGCDSTKQLRKAEEPSSLRVSEKNCTSERDLNIRVTDDDINEIIQIDGTGDNSSTEEMGSIRDADENEFPGIIDAGDLNVLEEVDSVSNEDSTANSSDNEDHQINAPEEDPLNSGDDVSEQDVPDLFDTENVIVCQYDKIHRSKNRWKFYLKDGVMCFGGRDYVFAKAIGEAEW

Target ORF information:

RefSeq Version	XM_011246628.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus general transcription factor IIA, 1-like (Gtf2a1l), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011246628.3

ORF Insert Sequence:

ATGGCCTTCC AGACGGCATC TGGTCACCTT TACAAAGTCA ATGTACCAGT CATGGTGACA 
CAGACTTCTG GGAGAACAGA AATTCTCCAG CATCCATTTC AGCAAGTCCT TCAGCAGCTC 
GGGCAGCCTT TAGTAATACA GACCACTGTT CCAACATTGC ACCCATGTTC TCTTCAAGCA 
GCAACTGAGA AATCCCTCAG AATGGAAGCT GTGCTGCAGC CACCTCCCAT TCTGCATCCT 
CCTCCAGTGG ACAGGACACA TGTAGAAAAT GCTGCGAGCG ACAGGCGCCT TCTCCCGGGA 
AATGAGCTGA GGCCGCAGGA AAGCTCTCCA TACCTCAGCC TTCCCGGTGT GGGCTTTCCT 
CCTCAGGCCG CTCTGACAGA GTCTAGCCTG GAGCCAGTGC TTGGTGTCTC AGCGAGCCTG 
ACTCAGAATC TGCACAGTGA CCCTTTCTCA CAGGGCCCCC CAGGCCCTCT CCACCACCAC 
TTGCTAGAGT CACAGCTTCA AAGCCTTAAA GACAGTATAT ATGGATGCGA CTCCACAAAG 
CAACTGAGAA AAGCAGAGGA GCCCAGCAGC CTCCGTGTGT CAGAGAAGAA TTGTACTTCA 
GAGAGGGATC TGAATATTCG GGTAACCGAT GATGATATTA ATGAAATAAT CCAAATAGAT 
GGAACCGGCG ATAACTCTTC TACTGAAGAG ATGGGAAGTA TAAGGGATGC AGATGAGAAT 
GAATTCCCAG GGATCATTGA TGCCGGCGAC CTCAATGTGC TTGAAGAAGT GGACAGCGTA 
TCGAATGAAG ATTCAACTGC AAATAGCAGC GACAACGAGG ACCATCAAAT AAATGCCCCA 
GAAGAGGATC CCCTAAATTC TGGCGATGAT GTCAGTGAGC AGGATGTGCC AGACCTGTTT 
GATACAGAGA ATGTAATTGT CTGTCAGTAT GATAAGATCC ACCGGAGCAA GAACAGATGG 
AAATTCTACT TGAAAGATGG TGTCATGTGC TTTGGAGGGA GAGACTACGT ATTTGCCAAA 
GCCATTGGTG AAGCTGAGTG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rfx2 is required for spermatogenesis in the mouse.
Genesis (New York, N.Y. : 2000)(2015 Aug)
Shawlot W,Vazquez-Chantada M,Wallingford JB,Finnell RH

Cleavage of TFIIA by Taspase1 activates TRF2-specified mammalian male germ cell programs.
Developmental cell27(2)188-200(2013 Oct)
Oyama T,Sasagawa S,Takeda S,Hess RA,Lieberman PM,Cheng EH,Hsieh JJ

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