Mipep cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mipep
Entrez Gene ID	70478
Full Name	mitochondrial intermediate peptidase
Synonyms	5730405E07Rik,MIP
General protein information	Preferred Names mitochondrial intermediate peptidase Names mitochondrial intermediate peptidase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006519508.2	XP_006519571.1	mitochondrial intermediate peptidase isoform X1
XM_006519508.3	XP_006519571.1	mitochondrial intermediate peptidase isoform X1
NM_027436.3	NP_081712.2	mitochondrial intermediate peptidase precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MIPEP	XP_003954496.1
Danio rerio (zebrafish)	mipep	XP_001344847.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007728	XP_308148.3
Schizosaccharomyces pombe (fission yeast)	oct1	NP_593013.1
Homo sapiens (human)	MIPEP	NP_005923.2
	MIPEP	XP_001090198.1
Canis lupus familiaris (dog)	MIPEP	XP_005635601.1
Rattus norvegicus (Norway rat)	Mipep	NP_112314.1
Drosophila melanogaster (fruit fly)	CG7791	NP_610183.1
Caenorhabditis elegans (roundworm)	CELE_Y67H2A.7	NP_502558.2
Kluyveromyces lactis	KLLA0B12397g	XP_452082.1
Gallus gallus (chicken)	MIPEP	XP_417136.2
Saccharomyces cerevisiae (baker's yeast)	OCT1	NP_012788.2
Eremothecium gossypii	AGOS_AFR198W	NP_985745.1
Oryza sativa (rice)	Os06g0686500	NP_001058401.1
Xenopus tropicalis (tropical clawed frog)	mipep	XP_004912119.1
Mus musculus (house mouse)	Mipep	NP_081712.2
Neurospora crassa	NCU02063	XP_964019.1
Bos taurus (cattle)	MIPEP	XP_002691892.1
Magnaporthe oryzae (rice blast fungus)	MGG_00487	XP_003718575.1
Arabidopsis thaliana (thale cress)	AT5G51540	NP_199967.2

Gene Ontology
Bibliography

Related articles in PubMed

Mitochondrial intermediate peptidase is a novel regulator of sirtuin-3 activation by caloric restriction.
Kobayashi M, Takeda K, Narita T, Nagai K, Okita N, Sudo Y, Miura Y, Tsumoto H, Nakagawa Y, Shimano H, Higami Y
FEBS letters591(24)4067-4073(2017 Dec)

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
G?mez-Del Arco P, Perdiguero E, Yunes-Leites PS, Ac?n-P?rez R, Zeini M, Garcia-Gomez A, Sreenivasan K, Jim?nez-Alc?zar M, Segal?s J, L?pez-Maderuelo D, Orn?s B, Jim?nez-Borreguero LJ, D'Amato G, Enshell-Seijffers D, Morgan B, Georgopoulos K, Islam AB, Braun T, de la Pompa JL, Kim J, Enriquez JA, Ballestar E, Mu?oz-C?noves P, Redondo JM
Cell metabolism23(5)881-92(2016 May)

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Mootha VK, Bunkenborg J, Olsen JV, Hjerrild M, Wisniewski JR, Stahl E, Bolouri MS, Ray HN, Sihag S, Kamal M, Patterson N, Lander ES, Mann M
Cell115(5)629-40(2003 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mipep gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mipep cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu50327	XM_006519508.3 Latest version!	Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu13104	NM_027436.3 Latest version!	Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OMu50327	XM_006519508.2	Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mipep ( XM_006519508.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519571.1 Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50327
Clone ID Related Accession (Same CDS sequence)	XM_006519508.2 , XM_006519508.3
Accession Version	XM_006519508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitochondrial intermediate peptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006519508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG CGGCCGGGGC ACGGTACGCC CGCCGGCTGT GCGGCCGGGG AGCCGCGGCG 
GCTCTGCAGG GACGCACAGG CCGGAGCTGC GCCCGGGACG TCAGTACTAG CTGGTCCCCG 
GTGGGCGCCG CCTTCAACGT CAAGCCCCAG AGCCACCTAT GGAACCTGTT GGGGGAGCGC 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAAGAG 
GAAGCCTTGA AGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC CCCAGGGCCA 
CAGACAGTCC TCATTTTTGA CGAACTCTCG GATTGCCTGT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGACCCT GCATTCAGAG AGGCTGCGCA AGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACAAACG TGGAGCTGTA TCAGAGTCTG 
CAAAGGTTAC TGGGTGATAA GAAGCTTATG GAGTCCCTTG ATGCGGAAAC CAGGCGAGTG 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCAG TGGACCTCAA TGTTAAGATC TTGGACTTGA GCAATGCTTT CCTCATGAGA 
ACCAACTTTC CCAACAAGAT CCGGAAGAGT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCCCGTGACG GAAGCCACTT AATCATTGAC GGCCTGCATG CAGAAGCCTC CGATGACTTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CCGATCAGTT GAAATGTTTA 
GAGGAATTGC TAAGCAGCAG AGACCTTCTG GCAAAATTAG TAGGTTATTC TACATTTTCC 
CACAGGGCCC TCCAAGGAAC CATAGCTCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTC TGAAAGAACT AGAAAAGATT TCAAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTAAA CTAATGCCTT GGGACCCTCC CTACTACAGC 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATACTGCCC GTTTTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC TTATTTAACA AACTGCTGGG CATCACCTTA 
TATGCAGAGC AGACCTTCAA AGGAGAGGTG TGGTGTAATG ACATCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGCT GCTGGGGTAC ATTTACTGTG ATTTTTTCCA GAGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGAGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTTC CAGTGGTCGT TCTCATGCTG AATCTTCCTC ATGCTTCCAG GGATTTCCCT 
ACATTATTAA CTCCAGGAAT GATGGAAAAT CTCTTCCATG AAATGGGCCA TGCCATGCAT 
TCAATGCTTG GACGAACTCG GTACCAACAT GTCACTGGGA GGAAATCTCA GGTGTCATTC 
TTGAATTTGT GCCCATTTAT ACTTTATGAT GACACAAAGA ACTTACACTT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519571.1
CDS	127..1746
Translation	MLLAAGARYARRLCGRGAAAALQGRTGRSCARDVSTSWSPVGAAFNVKPQSHLWNLLGERRGLFGVPELSTPEGFQVAQEEALKKTEWLVERACSTPPGPQTVLIFDELSDCLCRVADLADFVKIGHPDPAFREAAQEACRSIGTMVEKLNTNVELYQSLQRLLGDKKLMESLDAETRRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSNAFLMRTNFPNKIRKSLLPEHIQHHFARDGSHLIIDGLHAEASDDLVREAAYKIFLYPNADQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLSERTRKDFKMMQGMKTKLNPQNSKLMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVLFNKLLGITLYAEQTFKGEVWCNDIRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHVTGRKSQVSFLNLCPFILYDDTKNLHLCV

Target ORF information:

RefSeq Version	XM_006519508.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519508.3

ORF Insert Sequence:

ATGCTGCTGG CGGCCGGGGC ACGGTACGCC CGCCGGCTGT GCGGCCGGGG AGCCGCGGCG 
GCTCTGCAGG GACGCACAGG CCGGAGCTGC GCCCGGGACG TCAGTACTAG CTGGTCCCCG 
GTGGGCGCCG CCTTCAACGT CAAGCCCCAG AGCCACCTAT GGAACCTGTT GGGGGAGCGC 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAAGAG 
GAAGCCTTGA AGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC CCCAGGGCCA 
CAGACAGTCC TCATTTTTGA CGAACTCTCG GATTGCCTGT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGACCCT GCATTCAGAG AGGCTGCGCA AGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACAAACG TGGAGCTGTA TCAGAGTCTG 
CAAAGGTTAC TGGGTGATAA GAAGCTTATG GAGTCCCTTG ATGCGGAAAC CAGGCGAGTG 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCAG TGGACCTCAA TGTTAAGATC TTGGACTTGA GCAATGCTTT CCTCATGAGA 
ACCAACTTTC CCAACAAGAT CCGGAAGAGT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCCCGTGACG GAAGCCACTT AATCATTGAC GGCCTGCATG CAGAAGCCTC CGATGACTTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CCGATCAGTT GAAATGTTTA 
GAGGAATTGC TAAGCAGCAG AGACCTTCTG GCAAAATTAG TAGGTTATTC TACATTTTCC 
CACAGGGCCC TCCAAGGAAC CATAGCTCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTC TGAAAGAACT AGAAAAGATT TCAAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTAAA CTAATGCCTT GGGACCCTCC CTACTACAGC 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATACTGCCC GTTTTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC TTATTTAACA AACTGCTGGG CATCACCTTA 
TATGCAGAGC AGACCTTCAA AGGAGAGGTG TGGTGTAATG ACATCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGCT GCTGGGGTAC ATTTACTGTG ATTTTTTCCA GAGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGAGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTTC CAGTGGTCGT TCTCATGCTG AATCTTCCTC ATGCTTCCAG GGATTTCCCT 
ACATTATTAA CTCCAGGAAT GATGGAAAAT CTCTTCCATG AAATGGGCCA TGCCATGCAT 
TCAATGCTTG GACGAACTCG GTACCAACAT GTCACTGGGA GGAAATCTCA GGTGTCATTC 
TTGAATTTGT GCCCATTTAT ACTTTATGAT GACACAAAGA ACTTACACTT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mipep ( NM_027436.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081712.2 Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13104
Clone ID Related Accession (Same CDS sequence)	NM_027436.3
Accession Version	NM_027436.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	mitochondrial intermediate peptidase precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CA460475.1, AK017491.1, AI606287.1 and BE995975.1. On Nov 15, 2007 this sequence version replaced NM_027436.2. Transcript Variant: This variant (1) encodes the functional protein. ##Evidence-Data-START## Transcript exon combination :: BC110358.1, AK038479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGG CGGCCGGGGC ACGGTACGCC CGCCGGCTGT GCGGCCGGGG AGCCGCGGCG 
GCTCTGCAGG GACGCACAGG CCGGAGCTGC GCCCGGGACG TCAGTACTAG CTGGTCCCCG 
GTGGGCGCCG CCTTCAACGT CAAGCCCCAG AGCCACCTAT GGAACCTGTT GGGGGAGCGC 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAAGAG 
GAAGCCTTGA AGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC CCCAGGGCCA 
CAGACAGTCC TCATTTTTGA CGAACTCTCG GATTGCCTGT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGACCCT GCATTCAGAG AGGCTGCGCA AGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACAAACG TGGAGCTGTA TCAGAGTCTG 
CAAAGGTTAC TGGGTGATAA GAAGCTTATG GAGTCCCTTG ATGCGGAAAC CAGGCGAGTG 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCAG TGGACCTCAA TGTTAAGATC TTGGACTTGA GCAATGCTTT CCTCATGAGA 
ACCAACTTTC CCAACAAGAT CCGGAAGAGT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCCCGTGACG GAAGCCACTT AATCATTGAC GGCCTGCATG CAGAAGCCTC CGATGACTTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CCGATCAGTT GAAATGTTTA 
GAGGAATTGC TAAGCAGCAG AGACCTTCTG GCAAAATTAG TAGGTTATTC TACATTTTCC 
CACAGGGCCC TCCAAGGAAC CATAGCTCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTC TGAAAGAACT AGAAAAGATT TCAAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTAAA CTAATGCCTT GGGACCCTCC CTACTACAGC 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATACTGCCC GTTTTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC TTATTTAACA AACTGCTGGG CATCACCTTA 
TATGCAGAGC AGACCTTCAA AGGAGAGGTG TGGTGTAATG ACATCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGCT GCTGGGGTAC ATTTACTGTG ATTTTTTCCA GAGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGAGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTTC CAGTGGTCGT TCTCATGCTG AATCTTCCTC ATGCTTCCAG GGATTTCCCT 
ACATTATTAA CTCCAGGAAT GATGGAAAAT CTCTTCCATG AAATGGGCCA TGCCATGCAT 
TCAATGCTTG GACGAACTCG GTACCAACAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CATCTATTCT GATGGAGTAC TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTCGCCAAGC ATTATCAGAC TGGTCAGCCA CTCCCGAAAG CTATGGTGTC TCGTCTCTGT 
GAGTCTAAGA AGGTTTGCAC AGCAGCAGAA ATGCAGCTTC AGGTCTTTTA TGCCGCCCTG 
GATCAAATCT ACCATGGCCA GCATCCCCTG AAGAAGTCAA CCACAGATAT CCTCATGGAA 
ACACAAGAGC AGTTCTATGG CTTGCCCTAT GTTCCTGACA CTGCCTGGCA GCTGAGGTTC 
AGCCACCTCG TGGGATATGG TGCCAAGTAT TACTCATACC TGATGTCCAG GGCTGTGGCA 
TCCATGATCT GGAAGGAGTG CTTCCTACAG GATCCTTTTA ACAGGGCCGC TGGGGAACGC 
TACCGCAGAG AGATGTTGGC CCATGGTGGA GGCAAGGAGC CCATGCTTAT GATTCAAGGC 
ATGCTGCAGA AGTGTCCTTC CATCGATGAC TTTGTGGATG CCCTCGTGTC TGACATGAAT 
CTGGACTTTG AAACGTTCTT CCTGGATTCT AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081712.2
CDS	123..2258
Translation	MLLAAGARYARRLCGRGAAAALQGRTGRSCARDVSTSWSPVGAAFNVKPQSHLWNLLGERRGLFGVPELSTPEGFQVAQEEALKKTEWLVERACSTPPGPQTVLIFDELSDCLCRVADLADFVKIGHPDPAFREAAQEACRSIGTMVEKLNTNVELYQSLQRLLGDKKLMESLDAETRRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSNAFLMRTNFPNKIRKSLLPEHIQHHFARDGSHLIIDGLHAEASDDLVREAAYKIFLYPNADQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLSERTRKDFKMMQGMKTKLNPQNSKLMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVLFNKLLGITLYAEQTFKGEVWCNDIRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHVTGTRCPTDFAEVPSILMEYFSNDYRVVSQFAKHYQTGQPLPKAMVSRLCESKKVCTAAEMQLQVFYAALDQIYHGQHPLKKSTTDILMETQEQFYGLPYVPDTAWQLRFSHLVGYGAKYYSYLMSRAVASMIWKECFLQDPFNRAAGERYRREMLAHGGGKEPMLMIQGMLQKCPSIDDFVDALVSDMNLDFETFFLDSK

Target ORF information:

RefSeq Version	NM_027436.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027436.3

ORF Insert Sequence:

ATGCTGCTGG CGGCCGGGGC ACGGTACGCC CGCCGGCTGT GCGGCCGGGG AGCCGCGGCG 
GCTCTGCAGG GACGCACAGG CCGGAGCTGC GCCCGGGACG TCAGTACTAG CTGGTCCCCG 
GTGGGCGCCG CCTTCAACGT CAAGCCCCAG AGCCACCTAT GGAACCTGTT GGGGGAGCGC 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAAGAG 
GAAGCCTTGA AGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC CCCAGGGCCA 
CAGACAGTCC TCATTTTTGA CGAACTCTCG GATTGCCTGT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGACCCT GCATTCAGAG AGGCTGCGCA AGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACAAACG TGGAGCTGTA TCAGAGTCTG 
CAAAGGTTAC TGGGTGATAA GAAGCTTATG GAGTCCCTTG ATGCGGAAAC CAGGCGAGTG 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCAG TGGACCTCAA TGTTAAGATC TTGGACTTGA GCAATGCTTT CCTCATGAGA 
ACCAACTTTC CCAACAAGAT CCGGAAGAGT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCCCGTGACG GAAGCCACTT AATCATTGAC GGCCTGCATG CAGAAGCCTC CGATGACTTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CCGATCAGTT GAAATGTTTA 
GAGGAATTGC TAAGCAGCAG AGACCTTCTG GCAAAATTAG TAGGTTATTC TACATTTTCC 
CACAGGGCCC TCCAAGGAAC CATAGCTCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTC TGAAAGAACT AGAAAAGATT TCAAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTAAA CTAATGCCTT GGGACCCTCC CTACTACAGC 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATACTGCCC GTTTTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC TTATTTAACA AACTGCTGGG CATCACCTTA 
TATGCAGAGC AGACCTTCAA AGGAGAGGTG TGGTGTAATG ACATCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGCT GCTGGGGTAC ATTTACTGTG ATTTTTTCCA GAGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGAGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTTC CAGTGGTCGT TCTCATGCTG AATCTTCCTC ATGCTTCCAG GGATTTCCCT 
ACATTATTAA CTCCAGGAAT GATGGAAAAT CTCTTCCATG AAATGGGCCA TGCCATGCAT 
TCAATGCTTG GACGAACTCG GTACCAACAT GTCACTGGGA CCAGGTGCCC TACTGACTTT 
GCTGAAGTTC CATCTATTCT GATGGAGTAC TTTTCAAATG ATTACCGAGT AGTCAGCCAG 
TTCGCCAAGC ATTATCAGAC TGGTCAGCCA CTCCCGAAAG CTATGGTGTC TCGTCTCTGT 
GAGTCTAAGA AGGTTTGCAC AGCAGCAGAA ATGCAGCTTC AGGTCTTTTA TGCCGCCCTG 
GATCAAATCT ACCATGGCCA GCATCCCCTG AAGAAGTCAA CCACAGATAT CCTCATGGAA 
ACACAAGAGC AGTTCTATGG CTTGCCCTAT GTTCCTGACA CTGCCTGGCA GCTGAGGTTC 
AGCCACCTCG TGGGATATGG TGCCAAGTAT TACTCATACC TGATGTCCAG GGCTGTGGCA 
TCCATGATCT GGAAGGAGTG CTTCCTACAG GATCCTTTTA ACAGGGCCGC TGGGGAACGC 
TACCGCAGAG AGATGTTGGC CCATGGTGGA GGCAAGGAGC CCATGCTTAT GATTCAAGGC 
ATGCTGCAGA AGTGTCCTTC CATCGATGAC TTTGTGGATG CCCTCGTGTC TGACATGAAT 
CTGGACTTTG AAACGTTCTT CCTGGATTCT AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mipep ( XM_006519508.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006519571.1 Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu50327
Clone ID Related Accession (Same CDS sequence)	XM_006519508.2 , XM_006519508.3
Accession Version	XM_006519508.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	mitochondrial intermediate peptidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006519508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGG CGGCCGGGGC ACGGTACGCC CGCCGGCTGT GCGGCCGGGG AGCCGCGGCG 
GCTCTGCAGG GACGCACAGG CCGGAGCTGC GCCCGGGACG TCAGTACTAG CTGGTCCCCG 
GTGGGCGCCG CCTTCAACGT CAAGCCCCAG AGCCACCTAT GGAACCTGTT GGGGGAGCGC 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAAGAG 
GAAGCCTTGA AGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC CCCAGGGCCA 
CAGACAGTCC TCATTTTTGA CGAACTCTCG GATTGCCTGT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGACCCT GCATTCAGAG AGGCTGCGCA AGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACAAACG TGGAGCTGTA TCAGAGTCTG 
CAAAGGTTAC TGGGTGATAA GAAGCTTATG GAGTCCCTTG ATGCGGAAAC CAGGCGAGTG 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCAG TGGACCTCAA TGTTAAGATC TTGGACTTGA GCAATGCTTT CCTCATGAGA 
ACCAACTTTC CCAACAAGAT CCGGAAGAGT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCCCGTGACG GAAGCCACTT AATCATTGAC GGCCTGCATG CAGAAGCCTC CGATGACTTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CCGATCAGTT GAAATGTTTA 
GAGGAATTGC TAAGCAGCAG AGACCTTCTG GCAAAATTAG TAGGTTATTC TACATTTTCC 
CACAGGGCCC TCCAAGGAAC CATAGCTCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTC TGAAAGAACT AGAAAAGATT TCAAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTAAA CTAATGCCTT GGGACCCTCC CTACTACAGC 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATACTGCCC GTTTTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC TTATTTAACA AACTGCTGGG CATCACCTTA 
TATGCAGAGC AGACCTTCAA AGGAGAGGTG TGGTGTAATG ACATCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGCT GCTGGGGTAC ATTTACTGTG ATTTTTTCCA GAGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGAGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTTC CAGTGGTCGT TCTCATGCTG AATCTTCCTC ATGCTTCCAG GGATTTCCCT 
ACATTATTAA CTCCAGGAAT GATGGAAAAT CTCTTCCATG AAATGGGCCA TGCCATGCAT 
TCAATGCTTG GACGAACTCG GTACCAACAT GTCACTGGGA GGAAATCTCA GGTGTCATTC 
TTGAATTTGT GCCCATTTAT ACTTTATGAT GACACAAAGA ACTTACACTT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006519571.1
CDS	127..1746
Translation	MLLAAGARYARRLCGRGAAAALQGRTGRSCARDVSTSWSPVGAAFNVKPQSHLWNLLGERRGLFGVPELSTPEGFQVAQEEALKKTEWLVERACSTPPGPQTVLIFDELSDCLCRVADLADFVKIGHPDPAFREAAQEACRSIGTMVEKLNTNVELYQSLQRLLGDKKLMESLDAETRRVAELFMFDFEISGIHLDEEKRRRAVDLNVKILDLSNAFLMRTNFPNKIRKSLLPEHIQHHFARDGSHLIIDGLHAEASDDLVREAAYKIFLYPNADQLKCLEELLSSRDLLAKLVGYSTFSHRALQGTIAQTPETVMQFLEKLSEKLSERTRKDFKMMQGMKTKLNPQNSKLMPWDPPYYSGVIRAERYNIEPSLYCPFLSLGACMEGLNVLFNKLLGITLYAEQTFKGEVWCNDIRKLAVVHESEGLLGYIYCDFFQRANKPQQDCHFTIRGGRLKEDGSYQLPVVVLMLNLPHASRDFPTLLTPGMMENLFHEMGHAMHSMLGRTRYQHVTGRKSQVSFLNLCPFILYDDTKNLHLCV

Target ORF information:

RefSeq Version	XM_006519508.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mitochondrial intermediate peptidase (Mipep), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006519508.2

ORF Insert Sequence:

ATGCTGCTGG CGGCCGGGGC ACGGTACGCC CGCCGGCTGT GCGGCCGGGG AGCCGCGGCG 
GCTCTGCAGG GACGCACAGG CCGGAGCTGC GCCCGGGACG TCAGTACTAG CTGGTCCCCG 
GTGGGCGCCG CCTTCAACGT CAAGCCCCAG AGCCACCTAT GGAACCTGTT GGGGGAGCGC 
CGGGGTCTTT TTGGAGTTCC TGAGCTCAGC ACCCCTGAAG GATTCCAAGT TGCCCAAGAG 
GAAGCCTTGA AGAAGACGGA GTGGCTGGTA GAGCGAGCAT GTTCCACACC CCCAGGGCCA 
CAGACAGTCC TCATTTTTGA CGAACTCTCG GATTGCCTGT GCAGAGTGGC TGACTTGGCG 
GACTTTGTGA AAATTGGACA CCCAGACCCT GCATTCAGAG AGGCTGCGCA AGAGGCCTGC 
AGAAGTATTG GCACCATGGT GGAAAAGTTG AATACAAACG TGGAGCTGTA TCAGAGTCTG 
CAAAGGTTAC TGGGTGATAA GAAGCTTATG GAGTCCCTTG ATGCGGAAAC CAGGCGAGTG 
GCCGAACTAT TTATGTTTGA TTTTGAAATC AGTGGAATCC ATCTAGATGA AGAAAAGCGC 
AGAAGAGCAG TGGACCTCAA TGTTAAGATC TTGGACTTGA GCAATGCTTT CCTCATGAGA 
ACCAACTTTC CCAACAAGAT CCGGAAGAGT CTCTTACCAG AGCACATTCA GCACCACTTT 
GCCCGTGACG GAAGCCACTT AATCATTGAC GGCCTGCATG CAGAAGCCTC CGATGACTTG 
GTTCGGGAGG CTGCTTATAA AATTTTCCTT TATCCCAATG CCGATCAGTT GAAATGTTTA 
GAGGAATTGC TAAGCAGCAG AGACCTTCTG GCAAAATTAG TAGGTTATTC TACATTTTCC 
CACAGGGCCC TCCAAGGAAC CATAGCTCAG ACTCCAGAGA CTGTCATGCA GTTCCTTGAG 
AAGCTATCTG AAAAGCTCTC TGAAAGAACT AGAAAAGATT TCAAGATGAT GCAAGGAATG 
AAAACAAAAC TAAATCCTCA AAATTCTAAA CTAATGCCTT GGGACCCTCC CTACTACAGC 
GGTGTGATTC GTGCAGAGAG GTATAATATT GAGCCCAGTT TATACTGCCC GTTTTTATCC 
CTTGGAGCTT GCATGGAAGG CCTGAATGTC TTATTTAACA AACTGCTGGG CATCACCTTA 
TATGCAGAGC AGACCTTCAA AGGAGAGGTG TGGTGTAATG ACATCCGGAA GCTGGCTGTT 
GTTCATGAAT CTGAAGGGCT GCTGGGGTAC ATTTACTGTG ATTTTTTCCA GAGAGCAAAC 
AAGCCACAGC AGGATTGCCA CTTCACCATC CGAGGAGGCA GATTAAAGGA AGATGGCAGC 
TATCAGCTTC CAGTGGTCGT TCTCATGCTG AATCTTCCTC ATGCTTCCAG GGATTTCCCT 
ACATTATTAA CTCCAGGAAT GATGGAAAAT CTCTTCCATG AAATGGGCCA TGCCATGCAT 
TCAATGCTTG GACGAACTCG GTACCAACAT GTCACTGGGA GGAAATCTCA GGTGTCATTC 
TTGAATTTGT GCCCATTTAT ACTTTATGAT GACACAAAGA ACTTACACTT GTGTGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitochondrial intermediate peptidase is a novel regulator of sirtuin-3 activation by caloric restriction.
FEBS letters591(24)4067-4073(2017 Dec)
Kobayashi M,Takeda K,Narita T,Nagai K,Okita N,Sudo Y,Miura Y,Tsumoto H,Nakagawa Y,Shimano H,Higami Y

The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis.
Cell metabolism23(5)881-92(2016 May)
G?mez-Del Arco P,Perdiguero E,Yunes-Leites PS,Ac?n-P?rez R,Zeini M,Garcia-Gomez A,Sreenivasan K,Jim?nez-Alc?zar M,Segal?s J,L?pez-Maderuelo D,Orn?s B,Jim?nez-Borreguero LJ,D'Amato G,Enshell-Seijffers D,Morgan B,Georgopoulos K,Islam AB,Braun T,de la Pompa JL,Kim J,Enriquez JA,Ballestar E,Mu?oz-C?noves P,Redondo JM

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria.
Cell115(5)629-40(2003 Nov)
Mootha VK,Bunkenborg J,Olsen JV,Hjerrild M,Wisniewski JR,Stahl E,Bolouri MS,Ray HN,Sihag S,Kamal M,Patterson N,Lander ES,Mann M

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Mipep cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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