Cd3eap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cd3eap
Entrez Gene ID	70333
Full Name	CD3E antigen, epsilon polypeptide associated protein
Synonyms	1190028F09,2610103M17Rik,ASE-1,Ase1,CAST,PAF49
General protein information	Preferred Names CD3E antigen, epsilon polypeptide associated protein Names DNA-directed RNA polymerase I subunit RPA34 A34.5 CD3 epsilon associated protein; antisense to Ercc1 CD3-epsilon-associated protein CD3E-associated protein RNA polymerase I-associated factor PAF49 anti-sense to ERCC-1 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_145822.2	NP_665821.1	DNA-directed RNA polymerase I subunit RPA34

Pathways from BioSystems

Homologies

	CD3EAP	XP_001110546.2
Bos taurus (cattle)	CD3EAP	NP_001069953.1
Homo sapiens (human)	CD3EAP	NP_036231.1
Mus musculus (house mouse)	Cd3eap	NP_665821.1
Rattus norvegicus (Norway rat)	Cd3eap	NP_001102886.1
Pan troglodytes (chimpanzee)	CD3EAP	XP_001164895.2
Canis lupus familiaris (dog)	CD3EAP	XP_005616930.1

Gene Ontology
Bibliography

Related articles in PubMed

The tissue-specific transcriptomic landscape of the mid-gestational mouse embryo.
Werber M, Wittler L, Timmermann B, Grote P, Herrmann BG
Development (Cambridge, England)141(11)2325-30(2014 Jun)

Characterization of the interactions of mammalian RNA polymerase I associated proteins PAF53 and PAF49.
Penrod Y, Rothblum K, Rothblum LI
Biochemistry51(33)6519-26(2012 Aug)

Nucleolar targeting of proteins by the tandem array of basic amino acid stretches identified in the RNA polymerase I-associated factor PAF49.
Ushijima R, Matsuyama T, Nagata I, Yamamoto K
Biochemical and biophysical research communications369(4)1017-21(2008 May)

Phosphoproteomic analysis of the developing mouse brain.
Ballif BA, Vill?n J, Beausoleil SA, Schwartz D, Gygi SP
Molecular & cellular proteomics : MCP3(11)1093-101(2004 Nov)

Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.
Yamamoto K, Yamamoto M, Hanada K, Nogi Y, Matsuyama T, Muramatsu M
Molecular and cellular biology24(14)6338-49(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

yeast A34.5/A49 and mammalian PAF49/PAF53 heterodimeric complexes interact through species-specific sequence elements that appear to assume similar structural motifs.
Title: Characterization of the interactions of mammalian RNA polymerase I associated proteins PAF53 and PAF49.

Serial deletion analysis revealed that amino acids 199-338 were necessary for the nucleolar localization of PAF49.
Title: Nucleolar targeting of proteins by the tandem array of basic amino acid stretches identified in the RNA polymerase I-associated factor PAF49.

These results strongly suggest that RNA polymerase I (Pol I)-associated factor PAF49/ASE-1 plays an important role in rRNA transcription.
Title: Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.

The following Cd3eap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cd3eap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11969	NM_145822.2 Latest version!	Mus musculus CD3E antigen, epsilon polypeptide associated protein (Cd3eap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cd3eap ( NM_145822.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_665821.1 Mus musculus CD3E antigen, epsilon polypeptide associated protein (Cd3eap), mRNA.

Price & Availability↑

CloneID	OMu11969
Clone ID Related Accession (Same CDS sequence)	NM_145822.2
Accession Version	NM_145822.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	DNA-directed RNA polymerase I subunit RPA34
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC148988.5. On Feb 2, 2010 this sequence version replaced NM_145822.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC071199.1, AB091121.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164134 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGTA CCCAGGCCTG CAGTGCCACT CGGTTCTCCT GCCCTCCCCA CTTCACGGAG 
ATGTCGCCAG ACTCGGAACC CTCCCGTTTC TCCTTGGAGG CGCTTACAGG TCCAGATACA 
GAACTGTGGC TTATCCAAGC CCCTGCAGAC TTCGCCCCAC AATGTCTCAA TGGGCGGCGT 
GTACCTCTTT CTGGCTCCAA GACTGTTAAG GGCAAACTGG ATGGCAAGAA GCACCGATAC 
CGGGTATTTA CCAGCAGTCC CCAGGCCCGA GAAGCCACCT TGCTCGCATC TTCATCAGAG 
GCAGGTGGCA GACTCACCTG CGCCCCGGCC CCCAGTGGGA GCTTAAGGAT CATGGAGGGT 
CCTCAAGAAT ATCTTCTCTC TCGGGTCCCC CTGCAGCTGA TCCCTACAAG TCTGCCACCT 
CAGATCCCTG CTGGCCTGCG GCCTCGCTTC TCCGCCTTTG GCGGCAGCCC TCCCGTCACG 
GGGCCAGGTT CAGCCTCAGC TCTGAGGTCA CCAACTTCAG GGAAGAGGAA AAGTACAAGA 
AAGGGTACAG ATGCCTCCTC AGACACTCAG GAGGCTGTGA ACAGGCACGG GGCCATGGAA 
GTAAAAACAG CCTTGGGTAA CCTAGGAGTG AGTGTGAAGA AGAGAAAGAG GTATTTCATG 
CAGGAGGAGA TGGAAGCCAA GACAATGGAG CCCGTGGCAG AGCTGCCAGT CCCATCTGCC 
ACCAGCAGCA AGAAGAGGAA GAAGTCCAAG GGGACAGAGA CATCCCAGGT GGAGCACACA 
GAACCTGTGG CTCAAACAGA GCCTCCTGAG GGGACCTTCC TGTTCCCCAC CAAAAAGAGG 
AAGAGGCAGA AGGAAGCTGA TGGGACAGAG GAAGTGGATG GTATAGTGGC TGACTCTCAG 
CCACAGGTCA TTGTGGAAGC CCAGGAGGAA ACCATCCTCC TGTCCCCCAC AAAGAAGAGG 
AGAAAGGAAA AGAGACAGAA TTTGGGGATG GAGGCAGAGA TGGGGCCCCC TGGAGTACTC 
ATGGTGACCG AACATTCAGA ACATGGGCTC CAGGCTGAAG TAGCTCTTGT GTCCCCCAAG 
AAGACAAAGA AGAAAAAGGG AAAGCGGGTG GGTGAGACAG AGGCTGCACT TCCAGATGAC 
TTTGAGCCCC AGGGGGCTCT AGCACCCAGC AAGAAAAAAG AGAGAGGCGC ACGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665821.1
CDS	75..1274
Translation	MAGTQACSATRFSCPPHFTEMSPDSEPSRFSLEALTGPDTELWLIQAPADFAPQCLNGRRVPLSGSKTVKGKLDGKKHRYRVFTSSPQAREATLLASSSEAGGRLTCAPAPSGSLRIMEGPQEYLLSRVPLQLIPTSLPPQIPAGLRPRFSAFGGSPPVTGPGSASALRSPTSGKRKSTRKGTDASSDTQEAVNRHGAMEVKTALGNLGVSVKKRKRYFMQEEMEAKTMEPVAELPVPSATSSKKRKKSKGTETSQVEHTEPVAQTEPPEGTFLFPTKKRKRQKEADGTEEVDGIVADSQPQVIVEAQEETILLSPTKKRRKEKRQNLGMEAEMGPPGVLMVTEHSEHGLQAEVALVSPKKTKKKKGKRVGETEAALPDDFEPQGALAPSKKKERGARL

Target ORF information:

RefSeq Version	NM_145822.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus CD3E antigen, epsilon polypeptide associated protein (Cd3eap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145822.2

ORF Insert Sequence:

ATGGCGGGTA CCCAGGCCTG CAGTGCCACT CGGTTCTCCT GCCCTCCCCA CTTCACGGAG 
ATGTCGCCAG ACTCGGAACC CTCCCGTTTC TCCTTGGAGG CGCTTACAGG TCCAGATACA 
GAACTGTGGC TTATCCAAGC CCCTGCAGAC TTCGCCCCAC AATGTCTCAA TGGGCGGCGT 
GTACCTCTTT CTGGCTCCAA GACTGTTAAG GGCAAACTGG ATGGCAAGAA GCACCGATAC 
CGGGTATTTA CCAGCAGTCC CCAGGCCCGA GAAGCCACCT TGCTCGCATC TTCATCAGAG 
GCAGGTGGCA GACTCACCTG CGCCCCGGCC CCCAGTGGGA GCTTAAGGAT CATGGAGGGT 
CCTCAAGAAT ATCTTCTCTC TCGGGTCCCC CTGCAGCTGA TCCCTACAAG TCTGCCACCT 
CAGATCCCTG CTGGCCTGCG GCCTCGCTTC TCCGCCTTTG GCGGCAGCCC TCCCGTCACG 
GGGCCAGGTT CAGCCTCAGC TCTGAGGTCA CCAACTTCAG GGAAGAGGAA AAGTACAAGA 
AAGGGTACAG ATGCCTCCTC AGACACTCAG GAGGCTGTGA ACAGGCACGG GGCCATGGAA 
GTAAAAACAG CCTTGGGTAA CCTAGGAGTG AGTGTGAAGA AGAGAAAGAG GTATTTCATG 
CAGGAGGAGA TGGAAGCCAA GACAATGGAG CCCGTGGCAG AGCTGCCAGT CCCATCTGCC 
ACCAGCAGCA AGAAGAGGAA GAAGTCCAAG GGGACAGAGA CATCCCAGGT GGAGCACACA 
GAACCTGTGG CTCAAACAGA GCCTCCTGAG GGGACCTTCC TGTTCCCCAC CAAAAAGAGG 
AAGAGGCAGA AGGAAGCTGA TGGGACAGAG GAAGTGGATG GTATAGTGGC TGACTCTCAG 
CCACAGGTCA TTGTGGAAGC CCAGGAGGAA ACCATCCTCC TGTCCCCCAC AAAGAAGAGG 
AGAAAGGAAA AGAGACAGAA TTTGGGGATG GAGGCAGAGA TGGGGCCCCC TGGAGTACTC 
ATGGTGACCG AACATTCAGA ACATGGGCTC CAGGCTGAAG TAGCTCTTGT GTCCCCCAAG 
AAGACAAAGA AGAAAAAGGG AAAGCGGGTG GGTGAGACAG AGGCTGCACT TCCAGATGAC 
TTTGAGCCCC AGGGGGCTCT AGCACCCAGC AAGAAAAAAG AGAGAGGCGC ACGCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The tissue-specific transcriptomic landscape of the mid-gestational mouse embryo.
Development (Cambridge, England)141(11)2325-30(2014 Jun)
Werber M,Wittler L,Timmermann B,Grote P,Herrmann BG

Characterization of the interactions of mammalian RNA polymerase I associated proteins PAF53 and PAF49.
Biochemistry51(33)6519-26(2012 Aug)
Penrod Y,Rothblum K,Rothblum LI

Nucleolar targeting of proteins by the tandem array of basic amino acid stretches identified in the RNA polymerase I-associated factor PAF49.
Biochemical and biophysical research communications369(4)1017-21(2008 May)
Ushijima R,Matsuyama T,Nagata I,Yamamoto K

Phosphoproteomic analysis of the developing mouse brain.
Molecular & cellular proteomics : MCP3(11)1093-101(2004 Nov)
Ballif BA,Vill?n J,Beausoleil SA,Schwartz D,Gygi SP

Multiple protein-protein interactions by RNA polymerase I-associated factor PAF49 and role of PAF49 in rRNA transcription.
Molecular and cellular biology24(14)6338-49(2004 Jul)
Yamamoto K,Yamamoto M,Hanada K,Nogi Y,Matsuyama T,Muramatsu M