Afap1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Afap1
Entrez Gene ID	70292
Full Name	actin filament associated protein 1
Synonyms	2600003E23Rik,9630044L16Rik,AI848729,Afap,mKIAA3018
General protein information	Preferred Names actin filament associated protein 1 Names actin filament-associated protein 1 110 kDa actin filament-associated protein AFAP-110
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B3

mRNA and Protein(s)

mRNA	Protein	Name
NM_027373.2	NP_081649.1	actin filament-associated protein 1
XM_006504098.2	XP_006504161.1	actin filament-associated protein 1 isoform X1
XM_006504098.3	XP_006504161.1	actin filament-associated protein 1 isoform X1
XM_030254799.1	XP_030110659.1	actin filament-associated protein 1 isoform X2
XM_006504100.3	XP_006504163.1	actin filament-associated protein 1 isoform X2
XM_030254800.1	XP_030110660.1	actin filament-associated protein 1 isoform X3

Pathways from BioSystems

Homologies

	LOC709419	XP_002804046.1
Xenopus tropicalis (tropical clawed frog)	afap1	XP_004911301.1
Mus musculus (house mouse)	Afap1	NP_081649.1
Gallus gallus (chicken)	AFAP1	NP_989536.1
Bos taurus (cattle)	AFAP1	NP_001192973.1
Rattus norvegicus (Norway rat)	Afap1	NP_543176.1
Danio rerio (zebrafish)	afap1	XP_005156284.1
Homo sapiens (human)	AFAP1	NP_001128119.1
Pan troglodytes (chimpanzee)	AFAP1	XP_003310294.1
Canis lupus familiaris (dog)	AFAP1	XP_005618516.1

Gene Ontology
Bibliography

Related articles in PubMed

Actin filament-associated protein 1 is required for cSrc activity and secretory activation in the lactating mammary gland.
Cunnick JM, Kim S, Hadsell J, Collins S, Cerra C, Reiser P, Flynn DC, Cho Y
Oncogene34(20)2640-9(2015 May)

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Miller JA, Nathanson J, Franjic D, Shim S, Dalley RA, Shapouri S, Smith KA, Sunkin SM, Bernard A, Bennett JL, Lee CK, Hawrylycz MJ, Jones AR, Amaral DG, ?estan N, Gage FH, Lein ES
Development (Cambridge, England)140(22)4633-44(2013 Nov)

The protomap is propagated to cortical plate neurons through an Eomes-dependent intermediate map.
Elsen GE, Hodge RD, Bedogni F, Daza RA, Nelson BR, Shiba N, Reiner SL, Hevner RF
Proceedings of the National Academy of Sciences of the United States of America110(10)4081-6(2013 Mar)

AFAP120 regulates actin organization during neuronal differentiation.
Xu X, Harder J, Flynn DC, Lanier LM
Differentiation; research in biological diversity77(1)38-47(2009 Jan)

Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis.
Kawaguchi A, Ikawa T, Kasukawa T, Ueda HR, Kurimoto K, Saitou M, Matsuzaki F
Development (Cambridge, England)135(18)3113-24(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data define, for the first time, the requirement of AFAP1 for the spatial and temporal regulation of cSrc activity in the normal breast, specifically for milk production.
Title: Actin filament-associated protein 1 is required for cSrc activity and secretory activation in the lactating mammary gland.

These results indicate that AFAP120 plays a role in organizing dynamic actin structures during neuronal differentiation and suggest that AFAP120 may help regulate the transition from motile precursor to morphologically differentiated neurons.
Title: AFAP120 regulates actin organization during neuronal differentiation.

AFAP120 may coordinate Src signaling with the dynamic changes in the actin cytoskeleton that drive growth cone motility and axon elongation.
Title: The actin cross-linking protein AFAP120 regulates axon elongation in a tyrosine phosphorylation-dependent manner.

Actin filament associated protein-110 (AFAP) colocalizes with cSrc to promote cSrc activation and cell migration.
Title: PI3K activation is required for PMA-directed activation of cSrc by AFAP-110.

Data suggest that one major function of AFAP-110 is to relay signals from protein kinase C alpha that direct the activation of c-Src and the formation of podosomes.
Title: Protein kinase Calpha activates c-Src and induces podosome formation via AFAP-110.

The following Afap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Afap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu04097	NM_027373.2 Latest version!	Mus musculus actin filament associated protein 1 (Afap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OMu35188	XM_006504100.3 Latest version!	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu35186	XM_006504098.3 Latest version!	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu35188	XM_030254799.1 Latest version!	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu89013	XM_030254800.1 Latest version!	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu35186	XM_006504098.2	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Afap1 ( NM_027373.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081649.1 Mus musculus actin filament associated protein 1 (Afap1), mRNA.

Price & Availability↑

CloneID	OMu04097
Clone ID Related Accession (Same CDS sequence)	NM_027373.2
Accession Version	NM_027373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	actin filament-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC050814.1 and AC127275.4. On Nov 1, 2005 this sequence version replaced NM_027373.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK147706.1, AK129483.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA 
CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT 
CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC 
CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG 
CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG 
CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC 
AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG 
AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA 
CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC 
AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC 
AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG 
GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT 
GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG 
ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC 
ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA 
CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC GCCAGCGTCA 
TCTAATGGGG TTCCTGTAAA GGGGAAGGCT CCCAGCAGTC AGCAAAAAAA GGTGGAAACT 
GCAGGTGGTG TGAAACGGAC AGCATCAAAT GCTGAGCAGT ACAAATATGG GAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTGCTGAA GAGGAAAGAG 
GCCCTACGGA ACAGGCTGGC ACAGCTGCGG AAAGAGAGGA AGGACCTGAG GGCCGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGCA GCTCTGGAGG ACAAGTTGAA GAGGCTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCGGAGCGT GTCAGCCTGG AGCTGGAGCT GACCGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCGGGT GGCGTCACCC TAGGACTGGC CATCGAGCCC 
AGGTCAGGGA CATCCAGTCC ACAGTCTCCT GTGTTTCGGC ATCGGACTCT GGAAAATTCT 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCAGGC AGCTCCCCTT GCCGTGGCCA CGTGCTGCAG 
AAGGCCAAGG AGTGGGAGCT GAAGAATGGG ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081649.1
CDS	225..2420
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRLQSSKGFEVKDHAQKAEANNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSERPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSGSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	NM_027373.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin filament associated protein 1 (Afap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027373.2

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA 
CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT 
CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC 
CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG 
CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG 
CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC 
AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG 
AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA 
CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC 
AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC 
AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG 
GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT 
GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG 
ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC 
ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA 
CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTTGAAGA ACAAAAAACC GCCAGCGTCA 
TCTAATGGGG TTCCTGTAAA GGGGAAGGCT CCCAGCAGTC AGCAAAAAAA GGTGGAAACT 
GCAGGTGGTG TGAAACGGAC AGCATCAAAT GCTGAGCAGT ACAAATATGG GAAGAACCGG 
GTAGAAGCAG ATGCCAAGCG GTTACAGTCC AAAGAGGAAG AGCTGCTGAA GAGGAAAGAG 
GCCCTACGGA ACAGGCTGGC ACAGCTGCGG AAAGAGAGGA AGGACCTGAG GGCCGCCATC 
GAAGTGAATG CAGGCAGGAA GACCCAGGCA GCTCTGGAGG ACAAGTTGAA GAGGCTGGAG 
GAAGAGTGCA AGCAGAGGGA GGCGGAGCGT GTCAGCCTGG AGCTGGAGCT GACCGAGGTC 
AAGGAGAGCC TGAAGAAGGC CTTGGCGGGT GGCGTCACCC TAGGACTGGC CATCGAGCCC 
AGGTCAGGGA CATCCAGTCC ACAGTCTCCT GTGTTTCGGC ATCGGACTCT GGAAAATTCT 
CCCATCTCCA GCTGTGATAC AAGCGATGCT GAGGGGCCTT TGCCTGTGAA CAGTGCAGCC 
GTCCTGAAGA AGAGCCAGCC GTCCTCAGGC AGCTCCCCTT GCCGTGGCCA CGTGCTGCAG 
AAGGCCAAGG AGTGGGAGCT GAAGAATGGG ACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_006504098.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504161.1 Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35186
Clone ID Related Accession (Same CDS sequence)	XM_006504098.2 , XM_006504098.3
Accession Version	XM_006504098.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504098.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA 
CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT 
CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC 
CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG 
CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG 
CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC 
AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG 
AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA 
CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC 
AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC 
AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG 
GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT 
GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG 
ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC 
ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA 
CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC 
AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT 
GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA 
GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT 
CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC 
AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC 
GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG 
TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC 
TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504161.1
CDS	226..2688
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRLQSSKGFEVKDHAQKAEANNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSERPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSWDMDGGFPGCVSRGPVEEVLYDNAGLYDNLPSPTIFARSSPADRRASRPPTDRLSSNHYKSPTSCSPPCSPSVTNTSSVGRASLGLHSQLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSGSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_006504098.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504098.2

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA 
CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT 
CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC 
CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG 
CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG 
CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC 
AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG 
AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA 
CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC 
AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC 
AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG 
GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT 
GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG 
ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC 
ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA 
CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC 
AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT 
GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA 
GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT 
CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC 
AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC 
GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG 
TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC 
TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_006504100.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504163.1 Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu35188
Clone ID Related Accession (Same CDS sequence)	XM_030254799.1 , XM_006504100.3
Accession Version	XM_006504100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	actin filament-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC 
CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG 
ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC 
CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC 
AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA 
GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT 
GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG 
AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT 
GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT 
TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC 
ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT 
GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC 
AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC 
GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG 
ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA 
GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA 
GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA 
CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC 
TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC 
AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA 
GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT 
CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC 
CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT 
TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG 
TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA 
ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC 
CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA 
GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC 
ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG 
GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG 
GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG 
CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT 
TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA 
GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG 
CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504163.1
CDS	86..2224
Translation	MADYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSERPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSWDMDGGFPGCVSRGPVEEVLYDNAGLYDNLPSPTIFARSSPADRRASRPPTDRLSSNHYKSPTSCSPPCSPSVTNTSSVGRASLGLHSQLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSGSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_006504100.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504100.3

ORF Insert Sequence:

ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC 
CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG 
ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC 
CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC 
AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA 
GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT 
GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG 
AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT 
GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT 
TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC 
ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT 
GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC 
AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC 
GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG 
ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA 
GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA 
GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA 
CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC 
TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC 
AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA 
GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT 
CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC 
CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT 
TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG 
TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA 
ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC 
CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA 
GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC 
ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG 
GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG 
GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG 
CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT 
TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA 
GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG 
CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_006504098.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504161.1 Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35186
Clone ID Related Accession (Same CDS sequence)	XM_006504098.2 , XM_006504098.3
Accession Version	XM_006504098.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	actin filament-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504098.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA 
CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT 
CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC 
CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG 
CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG 
CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC 
AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG 
AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA 
CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC 
AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC 
AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG 
GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT 
GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG 
ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC 
ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA 
CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC 
AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT 
GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA 
GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT 
CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC 
AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC 
GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG 
TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC 
TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504161.1
CDS	221..2683
Translation	MEELIVELRLFLELLDHEYLTSTVREKKAVLTNILLRLQSSKGFEVKDHAQKAEANNLPAPPQMPLPEIPQPWLPPDSGPPPLPTSSLPEGYYEEAVPLSPGKAPEYITSNYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSERPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSWDMDGGFPGCVSRGPVEEVLYDNAGLYDNLPSPTIFARSSPADRRASRPPTDRLSSNHYKSPTSCSPPCSPSVTNTSSVGRASLGLHSQLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSGSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_006504098.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504098.3

ORF Insert Sequence:

ATGGAAGAGT TAATAGTTGA ACTCCGCCTC TTTCTGGAGC TCCTGGACCA CGAGTACCTC 
ACATCAACTG TCAGAGAGAA AAAGGCTGTG CTCACCAACA TCCTGCTGCG CCTGCAGTCA 
TCCAAGGGCT TTGAAGTGAA GGACCATGCT CAGAAGGCAG AGGCCAACAA CCTGCCGGCA 
CCACCCCAGA TGCCTCTGCC GGAGATCCCT CAGCCATGGC TGCCTCCTGA CAGCGGGCCT 
CCGCCTTTAC CAACGTCCTC TCTCCCAGAG GGTTACTATG AGGAAGCCGT GCCGCTGAGC 
CCTGGGAAAG CTCCAGAGTA CATCACCTCA AATTATGACT CTGATGCCAT GAGCAGTTCC 
TATGAGTCAT ATGATGAAGA GGAAGAGGAC GGGAAGGGGA AGAAAACTCG GCATCAGTGG 
CCGTCAGAGG AGGCCTCTAT GGACCTGGTG AAAGACGCCA AGATCTGTGC CTTCCTGCTG 
CGCAAGAAAC GCTTTGGCCA GTGGACCAAG CTGCTCTGCG TCATCAAAGA CACCAAGCTG 
CTGTGCTATA AAAGTTCCAA GGACCAGCAG CCTCAGATGG AGCTGCCGCT GCAGGGCTGC 
AGTATCACAT ACATCCCGAG AGACAGCAAG AAGAAGAAAC ACGAGCTGAA AATCACCCAG 
CAAGGCACAG ACCCTCTGGT TCTAGCTGTT CAGAGCAAGG AACAGGCTGA GCAGTGGTTG 
AAGGTAATCA AAGAAGCCTA CAGTGGCTGT AGCGGGCCCG TGGATCCGGA ATGTTCTCCA 
CCACCCAGCA CCAGCGCCCC CGTGAACAAG GCAGAACTGG AGAAGAAATT GTCTTCAGAG 
AGGCCCAGCT CTGATGGAGA AGGTGGTGTG GAAAACGGAG TCACCACGTG TAATGGAAAA 
GAACAAGCAA AGAGGAAGAA ACCTTCCAAG TCAGAGGCCA AGGGGACTGT GTCCAAGGTC 
ACTGGGAAGA AAATTACCAA GATCATCGGT CTGGGAAAGA AGAAGCCATC CACAGATGAG 
CAGACATCAT CAGCAGAGGA GGATGTGCCC ACTTGTGGCT ACCTGAATGT GCTCTCTAAC 
AGCCGCTGGC GGGAGCGCTG GTGCAGAGTG AAGGACAGCA AACTCATTCT CCACAAGGAC 
AGGGCTGACC TGAAGACCCA CCTCGTCTCC ATCCCCCTCC GTGGCTGCGA GGTGATCCCG 
GGCCTGGATT CTAAGCACCC GTTGACCTTC CGGCTGCTTC GAAACGGACA GGAGGTGGCT 
GTTCTAGAGG CATCATCGTC TGAAGACATG GGCAGGTGGA TTGGCATCTT GCTGGCTGAG 
ACGGGGTCCT CCACGGACCC AGGAGCGCTG CACTACGACT ACATCGATGT GGAGATGTCA 
GCAAATGTCA TCCAGACGGC GAAACAGACC TTCTGCTTTA TGAACAGGCG GGCAGTGTCC 
ACTAGCCCAT ACCTGGGGAG CCTCTCCAAC GGCTACGCCC ATCCCAGTGG GACAGCACTA 
CACTATGATG ACGTCCCCTG TGTCAACGGC TCGTGGGACA TGGATGGCGG CTTTCCTGGC 
TGTGTGAGCA GAGGCCCAGT GGAAGAGGTG CTTTATGATA ACGCGGGCCT CTACGATAAC 
TTGCCATCGC CGACAATCTT TGCTCGCTCC TCTCCTGCTG ACCGGAGGGC ATCTCGGCCA 
CCCACTGACA GGCTGTCCTC TAACCACTAC AAATCCCCCA CCTCCTGCAG CCCACCCTGC 
TCCCCATCTG TCACTAACAC CTCTTCTGTG GGGAGGGCAT CTCTCGGGCT GCACTCCCAG 
TTGAAGAACA AAAAACCGCC AGCGTCATCT AATGGGGTTC CTGTAAAGGG GAAGGCTCCC 
AGCAGTCAGC AAAAAAAGGT GGAAACTGCA GGTGGTGTGA AACGGACAGC ATCAAATGCT 
GAGCAGTACA AATATGGGAA GAACCGGGTA GAAGCAGATG CCAAGCGGTT ACAGTCCAAA 
GAGGAAGAGC TGCTGAAGAG GAAAGAGGCC CTACGGAACA GGCTGGCACA GCTGCGGAAA 
GAGAGGAAGG ACCTGAGGGC CGCCATCGAA GTGAATGCAG GCAGGAAGAC CCAGGCAGCT 
CTGGAGGACA AGTTGAAGAG GCTGGAGGAA GAGTGCAAGC AGAGGGAGGC GGAGCGTGTC 
AGCCTGGAGC TGGAGCTGAC CGAGGTCAAG GAGAGCCTGA AGAAGGCCTT GGCGGGTGGC 
GTCACCCTAG GACTGGCCAT CGAGCCCAGG TCAGGGACAT CCAGTCCACA GTCTCCTGTG 
TTTCGGCATC GGACTCTGGA AAATTCTCCC ATCTCCAGCT GTGATACAAG CGATGCTGAG 
GGGCCTTTGC CTGTGAACAG TGCAGCCGTC CTGAAGAAGA GCCAGCCGTC CTCAGGCAGC 
TCCCCTTGCC GTGGCCACGT GCTGCAGAAG GCCAAGGAGT GGGAGCTGAA GAATGGGACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_030254799.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110659.1 Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu35188
Clone ID Related Accession (Same CDS sequence)	XM_030254799.1 , XM_006504100.3
Accession Version	XM_030254799.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	actin filament-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC 
CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG 
ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC 
CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC 
AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA 
GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT 
GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG 
AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT 
GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT 
TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC 
ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT 
GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC 
AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC 
GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG 
ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA 
GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA 
GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA 
CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC 
TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC 
AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA 
GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT 
CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC 
CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT 
TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG 
TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA 
ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC 
CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA 
GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC 
ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG 
GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG 
GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG 
CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT 
TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA 
GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG 
CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110659.1
CDS	437..2575
Translation	MADYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSERPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSWDMDGGFPGCVSRGPVEEVLYDNAGLYDNLPSPTIFARSSPADRRASRPPTDRLSSNHYKSPTSCSPPCSPSVTNTSSVGRASLGLHSQLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSGSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_030254799.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254799.1

ORF Insert Sequence:

ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC 
CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG 
ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC 
CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC 
AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA 
GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT 
GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG 
AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT 
GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT 
TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC 
ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT 
GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC 
AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC 
GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG 
ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA 
GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA 
GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA 
CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC 
TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC 
AACGGCTCGT GGGACATGGA TGGCGGCTTT CCTGGCTGTG TGAGCAGAGG CCCAGTGGAA 
GAGGTGCTTT ATGATAACGC GGGCCTCTAC GATAACTTGC CATCGCCGAC AATCTTTGCT 
CGCTCCTCTC CTGCTGACCG GAGGGCATCT CGGCCACCCA CTGACAGGCT GTCCTCTAAC 
CACTACAAAT CCCCCACCTC CTGCAGCCCA CCCTGCTCCC CATCTGTCAC TAACACCTCT 
TCTGTGGGGA GGGCATCTCT CGGGCTGCAC TCCCAGTTGA AGAACAAAAA ACCGCCAGCG 
TCATCTAATG GGGTTCCTGT AAAGGGGAAG GCTCCCAGCA GTCAGCAAAA AAAGGTGGAA 
ACTGCAGGTG GTGTGAAACG GACAGCATCA AATGCTGAGC AGTACAAATA TGGGAAGAAC 
CGGGTAGAAG CAGATGCCAA GCGGTTACAG TCCAAAGAGG AAGAGCTGCT GAAGAGGAAA 
GAGGCCCTAC GGAACAGGCT GGCACAGCTG CGGAAAGAGA GGAAGGACCT GAGGGCCGCC 
ATCGAAGTGA ATGCAGGCAG GAAGACCCAG GCAGCTCTGG AGGACAAGTT GAAGAGGCTG 
GAGGAAGAGT GCAAGCAGAG GGAGGCGGAG CGTGTCAGCC TGGAGCTGGA GCTGACCGAG 
GTCAAGGAGA GCCTGAAGAA GGCCTTGGCG GGTGGCGTCA CCCTAGGACT GGCCATCGAG 
CCCAGGTCAG GGACATCCAG TCCACAGTCT CCTGTGTTTC GGCATCGGAC TCTGGAAAAT 
TCTCCCATCT CCAGCTGTGA TACAAGCGAT GCTGAGGGGC CTTTGCCTGT GAACAGTGCA 
GCCGTCCTGA AGAAGAGCCA GCCGTCCTCA GGCAGCTCCC CTTGCCGTGG CCACGTGCTG 
CAGAAGGCCA AGGAGTGGGA GCTGAAGAAT GGGACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Afap1 ( XM_030254800.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030110660.1 Mus musculus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA.

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CloneID	OMu89013
Clone ID Related Accession (Same CDS sequence)	XM_030254800.1
Accession Version	XM_030254800.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	actin filament-associated protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC 
CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG 
ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC 
CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC 
AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA 
GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT 
GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG 
AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT 
GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT 
TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC 
ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT 
GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC 
AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC 
GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG 
ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA 
GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA 
GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA 
CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC 
TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC 
AACGGCTCGT TGAAGAACAA AAAACCGCCA GCGTCATCTA ATGGGGTTCC TGTAAAGGGG 
AAGGCTCCCA GCAGTCAGCA AAAAAAGGTG GAAACTGCAG GTGGTGTGAA ACGGACAGCA 
TCAAATGCTG AGCAGTACAA ATATGGGAAG AACCGGGTAG AAGCAGATGC CAAGCGGTTA 
CAGTCCAAAG AGGAAGAGCT GCTGAAGAGG AAAGAGGCCC TACGGAACAG GCTGGCACAG 
CTGCGGAAAG AGAGGAAGGA CCTGAGGGCC GCCATCGAAG TGAATGCAGG CAGGAAGACC 
CAGGCAGCTC TGGAGGACAA GTTGAAGAGG CTGGAGGAAG AGTGCAAGCA GAGGGAGGCG 
GAGCGTGTCA GCCTGGAGCT GGAGCTGACC GAGGTCAAGG AGAGCCTGAA GAAGGCCTTG 
GCGGGTGGCG TCACCCTAGG ACTGGCCATC GAGCCCAGGT CAGGGACATC CAGTCCACAG 
TCTCCTGTGT TTCGGCATCG GACTCTGGAA AATTCTCCCA TCTCCAGCTG TGATACAAGC 
GATGCTGAGG GGCCTTTGCC TGTGAACAGT GCAGCCGTCC TGAAGAAGAG CCAGCCGTCC 
TCAGGCAGCT CCCCTTGCCG TGGCCACGTG CTGCAGAAGG CCAAGGAGTG GGAGCTGAAG 
AATGGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030110660.1
CDS	436..2307
Translation	MADYDSDAMSSSYESYDEEEEDGKGKKTRHQWPSEEASMDLVKDAKICAFLLRKKRFGQWTKLLCVIKDTKLLCYKSSKDQQPQMELPLQGCSITYIPRDSKKKKHELKITQQGTDPLVLAVQSKEQAEQWLKVIKEAYSGCSGPVDPECSPPPSTSAPVNKAELEKKLSSERPSSDGEGGVENGVTTCNGKEQAKRKKPSKSEAKGTVSKVTGKKITKIIGLGKKKPSTDEQTSSAEEDVPTCGYLNVLSNSRWRERWCRVKDSKLILHKDRADLKTHLVSIPLRGCEVIPGLDSKHPLTFRLLRNGQEVAVLEASSSEDMGRWIGILLAETGSSTDPGALHYDYIDVEMSANVIQTAKQTFCFMNRRAVSTSPYLGSLSNGYAHPSGTALHYDDVPCVNGSLKNKKPPASSNGVPVKGKAPSSQQKKVETAGGVKRTASNAEQYKYGKNRVEADAKRLQSKEEELLKRKEALRNRLAQLRKERKDLRAAIEVNAGRKTQAALEDKLKRLEEECKQREAERVSLELELTEVKESLKKALAGGVTLGLAIEPRSGTSSPQSPVFRHRTLENSPISSCDTSDAEGPLPVNSAAVLKKSQPSSGSSPCRGHVLQKAKEWELKNGT

Target ORF information:

RefSeq Version	XM_030254800.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin filament associated protein 1 (Afap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030254800.1

ORF Insert Sequence:

ATGGCTGATT ATGACTCTGA TGCCATGAGC AGTTCCTATG AGTCATATGA TGAAGAGGAA 
GAGGACGGGA AGGGGAAGAA AACTCGGCAT CAGTGGCCGT CAGAGGAGGC CTCTATGGAC 
CTGGTGAAAG ACGCCAAGAT CTGTGCCTTC CTGCTGCGCA AGAAACGCTT TGGCCAGTGG 
ACCAAGCTGC TCTGCGTCAT CAAAGACACC AAGCTGCTGT GCTATAAAAG TTCCAAGGAC 
CAGCAGCCTC AGATGGAGCT GCCGCTGCAG GGCTGCAGTA TCACATACAT CCCGAGAGAC 
AGCAAGAAGA AGAAACACGA GCTGAAAATC ACCCAGCAAG GCACAGACCC TCTGGTTCTA 
GCTGTTCAGA GCAAGGAACA GGCTGAGCAG TGGTTGAAGG TAATCAAAGA AGCCTACAGT 
GGCTGTAGCG GGCCCGTGGA TCCGGAATGT TCTCCACCAC CCAGCACCAG CGCCCCCGTG 
AACAAGGCAG AACTGGAGAA GAAATTGTCT TCAGAGAGGC CCAGCTCTGA TGGAGAAGGT 
GGTGTGGAAA ACGGAGTCAC CACGTGTAAT GGAAAAGAAC AAGCAAAGAG GAAGAAACCT 
TCCAAGTCAG AGGCCAAGGG GACTGTGTCC AAGGTCACTG GGAAGAAAAT TACCAAGATC 
ATCGGTCTGG GAAAGAAGAA GCCATCCACA GATGAGCAGA CATCATCAGC AGAGGAGGAT 
GTGCCCACTT GTGGCTACCT GAATGTGCTC TCTAACAGCC GCTGGCGGGA GCGCTGGTGC 
AGAGTGAAGG ACAGCAAACT CATTCTCCAC AAGGACAGGG CTGACCTGAA GACCCACCTC 
GTCTCCATCC CCCTCCGTGG CTGCGAGGTG ATCCCGGGCC TGGATTCTAA GCACCCGTTG 
ACCTTCCGGC TGCTTCGAAA CGGACAGGAG GTGGCTGTTC TAGAGGCATC ATCGTCTGAA 
GACATGGGCA GGTGGATTGG CATCTTGCTG GCTGAGACGG GGTCCTCCAC GGACCCAGGA 
GCGCTGCACT ACGACTACAT CGATGTGGAG ATGTCAGCAA ATGTCATCCA GACGGCGAAA 
CAGACCTTCT GCTTTATGAA CAGGCGGGCA GTGTCCACTA GCCCATACCT GGGGAGCCTC 
TCCAACGGCT ACGCCCATCC CAGTGGGACA GCACTACACT ATGATGACGT CCCCTGTGTC 
AACGGCTCGT TGAAGAACAA AAAACCGCCA GCGTCATCTA ATGGGGTTCC TGTAAAGGGG 
AAGGCTCCCA GCAGTCAGCA AAAAAAGGTG GAAACTGCAG GTGGTGTGAA ACGGACAGCA 
TCAAATGCTG AGCAGTACAA ATATGGGAAG AACCGGGTAG AAGCAGATGC CAAGCGGTTA 
CAGTCCAAAG AGGAAGAGCT GCTGAAGAGG AAAGAGGCCC TACGGAACAG GCTGGCACAG 
CTGCGGAAAG AGAGGAAGGA CCTGAGGGCC GCCATCGAAG TGAATGCAGG CAGGAAGACC 
CAGGCAGCTC TGGAGGACAA GTTGAAGAGG CTGGAGGAAG AGTGCAAGCA GAGGGAGGCG 
GAGCGTGTCA GCCTGGAGCT GGAGCTGACC GAGGTCAAGG AGAGCCTGAA GAAGGCCTTG 
GCGGGTGGCG TCACCCTAGG ACTGGCCATC GAGCCCAGGT CAGGGACATC CAGTCCACAG 
TCTCCTGTGT TTCGGCATCG GACTCTGGAA AATTCTCCCA TCTCCAGCTG TGATACAAGC 
GATGCTGAGG GGCCTTTGCC TGTGAACAGT GCAGCCGTCC TGAAGAAGAG CCAGCCGTCC 
TCAGGCAGCT CCCCTTGCCG TGGCCACGTG CTGCAGAAGG CCAAGGAGTG GGAGCTGAAG 
AATGGGACAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Actin filament-associated protein 1 is required for cSrc activity and secretory activation in the lactating mammary gland.
Oncogene34(20)2640-9(2015 May)
Cunnick JM,Kim S,Hadsell J,Collins S,Cerra C,Reiser P,Flynn DC,Cho Y

Conserved molecular signatures of neurogenesis in the hippocampal subgranular zone of rodents and primates.
Development (Cambridge, England)140(22)4633-44(2013 Nov)
Miller JA,Nathanson J,Franjic D,Shim S,Dalley RA,Shapouri S,Smith KA,Sunkin SM,Bernard A,Bennett JL,Lee CK,Hawrylycz MJ,Jones AR,Amaral DG,?estan N,Gage FH,Lein ES

The protomap is propagated to cortical plate neurons through an Eomes-dependent intermediate map.
Proceedings of the National Academy of Sciences of the United States of America110(10)4081-6(2013 Mar)
Elsen GE,Hodge RD,Bedogni F,Daza RA,Nelson BR,Shiba N,Reiner SL,Hevner RF

AFAP120 regulates actin organization during neuronal differentiation.
Differentiation; research in biological diversity77(1)38-47(2009 Jan)
Xu X,Harder J,Flynn DC,Lanier LM

Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis.
Development (Cambridge, England)135(18)3113-24(2008 Sep)
Kawaguchi A,Ikawa T,Kasukawa T,Ueda HR,Kurimoto K,Saitou M,Matsuzaki F

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Afap1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

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FAX

オンラインフォーム