Med23 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med23
Entrez Gene ID	70208
Full Name	mediator complex subunit 23
Synonyms	130kDa,3000002A17Rik,Crsp3,ESTM7,Sur2,X83317,mKIAA1216
General protein information	Preferred Names mediator complex subunit 23 Names mediator of RNA polymerase II transcription subunit 23 CRSP complex subunit 3 cofactor required for Sp1 transcriptional activation subunit 3 cofactor required for Sp1 transcriptional activation, subunit 3 (130kD) mSur-2 protein sur-2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 A4\|10 12.26 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001166416.1	NP_001159888.1	mediator of RNA polymerase II transcription subunit 23 isoform 1
NM_027347.3	NP_081623.3	mediator of RNA polymerase II transcription subunit 23 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Med23	NP_001159888.1
Gallus gallus (chicken)	MED23	XP_424537.3
Caenorhabditis elegans (roundworm)	sur-2	NP_493575.2
Bos taurus (cattle)	MED23	NP_001192691.1
Homo sapiens (human)	MED23	NP_004821.2
Rattus norvegicus (Norway rat)	Med23	NP_001263983.1
Pan troglodytes (chimpanzee)	MED23	XP_527503.3
Canis lupus familiaris (dog)	MED23	XP_005627145.1
	MED23	XP_002803940.1
Danio rerio (zebrafish)	med23	NP_001003990.1
Drosophila melanogaster (fruit fly)	MED23	NP_001137741.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006046	XP_316095.4
Xenopus tropicalis (tropical clawed frog)	med23	XP_002936353.2

Gene Ontology
Bibliography

Related articles in PubMed

MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.
Aranda-Orgilles B, Salda?a-Meyer R, Wang E, Trompouki E, Fassl A, Lau S, Mullenders J, Rocha PP, Raviram R, Guillamot M, S?nchez-D?az M, Wang K, Kayembe C, Zhang N, Amoasii L, Choudhuri A, Skok JA, Schober M, Reinberg D, Sicinski P, Schrewe H, Tsirigos A, Zon LI, Aifantis I
Cell stem cell19(6)784-799(2016 Dec)

Mediator MED23 cooperates with RUNX2 to drive osteoblast differentiation and bone development.
Liu Z, Yao X, Yan G, Xu Y, Yan J, Zou W, Wang G
Nature communications711149(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MED23 constitutes a molecular node in the regulatory network of anabolic bone formation and related diseases.
Title: Mediator MED23 cooperates with RUNX2 to drive osteoblast differentiation and bone development.

Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling.
Title: Mediator Med23 deficiency enhances neural differentiation of murine embryonic stem cells through modulating BMP signaling.

The hepatic Med23 deletion impaired the Mediator and RNAPII recruitment and attenuated the expression of FOXO1 target genes.
Title: Liver Med23 ablation improves glucose and lipid metabolism through modulating FOXO1 activity.

Med23 contributes to controlling T-cell activation at the transcriptional level and prevents the development of autoimmunity.
Title: The mediator subunit Med23 contributes to controlling T-cell activation and prevents autoimmunity.

Data show that Kruppel-like transcription factors KLF2 expression is decreased in mediator complex Med23 null thymocytes.
Title: T-cells null for the MED23 subunit of mediator express decreased levels of KLF2 and inefficiently populate the peripheral lymphoid organs.

The following Med23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu04072	NM_027347.3 Latest version!	Mus musculus mediator complex subunit 23 (Med23), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OMu10419	NM_001166416.1 Latest version!	Mus musculus mediator complex subunit 23 (Med23), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med23 ( NM_027347.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081623.3 Mus musculus mediator complex subunit 23 (Med23), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu04072
Clone ID Related Accession (Same CDS sequence)	NM_027347.3
Accession Version	NM_027347.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 23 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ100894.1, AK129312.1 and BQ885176.1. On Oct 23, 2009 this sequence version replaced NM_027347.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050916.1, AK129312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGTACCGA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT 
ATAGAAGAGG CCTTCCCAGG AATGTTTATG GATACCCCCG AGGATGAAAA AACAAAACTA 
ATTAGCTGCT TGGCGGCCTT CCGGCAGTTT TGGAGTGGAC TTTCTCAGGA GTCTCATGAA 
CAGTGTGTTC AGTGGATTGT TAAATTTATT CATGGCCAAC ACAGCCCCAA AAGAATTTCT 
TTCCTCTATG ACTGCTTAGC AATGGCAGTT GAGACTGGTC TCCTTCCACC CAGGATGGTT 
TGTGAATCTT TGATAAACTC TGACTCTCTT GAATGGGAAA GAACACAGCT TTGGGCCTTA 
ACATTTAAGC TGGTTCGGAA AATCATTGGA GGAGTGGATT ACAAGGGTGT TCGAGACCTT 
CTCAAAGCGA TTTTGGAGAA AATCTTGACA ATCCCAAATA CAGTGAGCTC TGCTGTTGTC 
CAGCAGCTTC TGGCAGCAAG AGAGGTTATA GCTTATATCT TAGAACGAAA TGCCTGCCTA 
TTGCCGGCCT ACTTTGCAGT CACAGAGATC AGAAAACTAT ATCCTGAGGG AAAACTTCCA 
CACTGGTTAC TAGGAAACTT GGTATCAGAC TTTGTGGATA CCTTCAGGCC CACAGCAAGG 
ATTAACTCCA TTTGTGGCCG ATGCAGTCTT CTGCCAGTTG TGAACAACTC GGGAGCCATC 
TGTAACTCAT GGAAACTGGA CCCTGCAACT CTGCGATTTC CTTTGAAAGG CCTTTTGCCA 
TATGATAAGG ATCTGTTTGA ACCGCAGACT GCTTTGTTGA GATATGTACT GGAGCAACCT 
TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGCACAA GCAGCGCTGC 
CCTGTGCTGG AGGACCAGTT GGTGGACCTG GTGGTGTACG CCATGGAGAG GTCAGAGACC 
GAGGAGAAGT TTGACGATGG GGGAACCAGC CAGCTCCTGT GGCAGCACCT TTCCAGCCAG 
CTCATTTTCT TTGTGCTTTT CCAGTTTGCC AGTTTCCCAC ATATGGTCCT CTCTCTCCAC 
CAGAAGTTAG CAGGGCGAGG ACTGATTAAA GGGCGAGACC ATCTGATGTG GGTGCTGCTG 
CAGTTCATTT CTGGAAGTAT TCAGAAAAAT GCCCTCGCTG ACTTCCTCCC TGTCATGAAG 
CTCTTTGACC TGCTGTACCC GGAGAAGGAG TGTATCCCAG TTCCTGATAT TAACAAACCC 
CAGTCAACGC ACGCCTTTGC AATGACTTGT ATTTGGATTC ATCTTAATAG AAAAGCTCAA 
AATGGCGACT CCACGCTCCA GATCCCAATA CCCCATTCCC TGAAGCTGCA CCATGAGTTC 
CTCCAGCAGA GTCTGAGAAA TAAAAGCTTA CAGATGAATG ACTATAAGAT TGCCCTGCTA 
TGTAACGCGT ATTCCACAAA CTCAGAGTGT TTTACGTTAC CTATGGGAGC TCTGGTAGAA 
ACTATTTATG GAAATGGAAT TATGAGAGTG CCTCTCCCTG GAACGAGCTG TTTGGCCTCA 
GCATCAGTGA CTCCCTTACC GATGAACCTC CTGGATTCAC TGACGGTGCA TGCCAAGATG 
AGCCTTATTC ACAGCATTGC AACCAGAGTG ATAAAACTGG CTCATACGAA ATCCAGTGTT 
GCGCTGGCTC CAGCCCTAGT GGAAACTTAC AGCCGTTTAC TGGTCTACAT GGAAATTGAG 
TCTTTGGGCA TCAAAGGATT TATCAGTCAG CTCCTGCCCA CTGTCTTCAA GTCCCACGCC 
TGGGGCATCC TGCACACACT GCTGGAGATG TTCAGCCACC GAATGCACCA CATTCAGCCC 
CACTACCGAG TTCAGCTCCT GAGCCATCTC CACACACTGG CTGCAGTCGC ACAGACCAAC 
CAGAACCAGC TCCATCTGTG TGTGGAGAGC ACTGCACTGA GGCTCATCAC AGCCCTGGGA 
AGCTCAGAGG TCCAGCCGCA GTTCACGCGT TTCCTCAATG ATCCCAAAAC AGTGCTGTCC 
GCCGAATCTG AAGAGCTGAA CCGAGCCTTG ATCCTGACCT TAGCCAGAGC AACCCACGTC 
ACAGATTTTT TTACAGGATC TGATTCAATA CAGGGAACTT GGTGTAAAGA TATCCTTCAG 
ACCATCATGA ACTTTACTCC TCATAACTGG GCTTCACATA CCCTTAGCTG TTTTCCAGCC 
CCTCTGCAGG CTTTCTTCAA GCAAAATAAC GTGCCCCAGG AAAGCCGTTT TAATCTGAAG 
AAGAATGTGG AAGAAGAGTA CAGGAAGTGG AAGTCAATGA CTGATGAAAA CGAGATCATC 
ACCCAGTTCT CTGTGCAGGG CTTCCCTCCA CTCTTCCTCT GTCTCCTCTG GAAGATGCTC 
CTGGAGACGG ATCATATTAG TCAGATCGGC TACAAGGTCC TGGAGAGGAT TGGGGCCAGG 
GCCCTGGTGG CCCATGTGAG AACATTTGCA GATTTCCTGG TGTATGAGTT CTCTACGTCA 
GCAGGAGGTC AGCAACTCAA TAAGTGCATT GAAATTCTCA ACGACATGGT GTGGAAGTAC 
AACATTGTCA CGCTGGACAG ACTCATTCTC TGCCTGGCTA TGCGTAGTCA TGAAGGAAAT 
GAAGCCCAGG TTTGTTATTT CATAATTCAG TTGCTGTTGC TCAAACCAAA TGACTTCAGA 
AACCGAGTAA GTGACTTTGT GAAGGAGAAT TCCCCAGAGC ACTGGCTCCA GAGTGACTGG 
CACACCAAGC ACATGAGCTA CCACAAGAAA TATCCCGAGA AGCTGTATTT TGAAGGCCTG 
GCAGAGCAGG TTGACCCTCC TGTACCAATC CAGTCCCCTT ATCTGCCCAT CTACTTTGGA 
AATGTGTGTC TCCGGTTCCT TCCAGTATTT GATATAGTAA TCCACAGGTT TTTAGAGTTG 
CTTCCAGTGT CCAAATCACT AGAGACTCTC CTGGATCACC TAGGGGGCTT ATATAAATTC 
CATGACCGGC CAGTGACTTA TCTGTATAAT ACTTTGCACT ATTATGAAAT GTGCCTGAGA 
AACCGTGACC ATCTCAAACG AAAACTTGTC CATGCAATCA TTGGCTCCCT CAAAGATAAC 
CGGCCACAGG GCTGGTGTCT CAGTGATACC TACCTGAAGC ATGCTATGAA TGCACGGGAG 
GACAACCCCT GGGTTCCGGA GGACTCCTAC TACTGCAAGC TGATTGGCCG ACTGGTAGAC 
ACCATGGCGG GCAAGTCTCC AGGACCCTTC CCAAACTGTG ACTGGAGATT CAATGAGTTT 
CCCAACCCCG CTGCCCACGC TCTGCATGTG ACGTGTGTGG AACTCATGGC CTTGGCGGTT 
CCAGGCAAAG ATGTGGGGAA TGCCCTTCTC AATGTCGTCC TGAAAAGCCA GCCCTTAGTG 
CCAAGGGAGA ACATTACAGC GTGGATGAAT GCAATTGGTC TGATCATCAC GGCCCTCCCA 
GAGCCGTACT GGATTGTGCT TCATGACCGA ATTGTGAATG TGATCAGCAG TTCTAGCCTG 
ACGTCAGAGA CAGAGTGGGT CGGTTACCCG TTCCGCCTCT TCGACTTCAC CGCTTGTCAC 
CAGTCCTACT CGGAGATGAG CTGCAGCTAT ACGTTAGCTC TAGCCCATGC CGTGTGGCAC 
CACTCCAGCA TCGGGCAGCT CTCGCTTATC CCAAAGTTTC TCACTGAGGC GCTTCTCCCG 
GTGGTGAAGA CTGAGTTCCA GTTGCTCTAC GTGTACCATC TTGTGGGGCC ATTTCTACAG 
AGGTTCCAAC AGGAGAGAAC CCGGTGCATG ATTGAGATTG GCGTGGCATT TTATGACATG 
CTGTTGAATG TAGACCAGTG CAGCACGCAC TTAAATTACA TGGACCCCAT CTGCGACTTC 
CTGTATCATA TGAAGTATAT GTTTACTGGT GACAGTGTGA AAGAGCAAGT AGAGAAGATT 
ATCTGTAATT TAAAGCCGGC TTTAAAGCTT CGTCTTCGAT TCATCACGCA CATTAGCAAG 
ATGGAGCCGG CAGTGCCACC CCAGGCCCTC AACAGCGGGT CTCCAGCACC TCAGTCTAAC 
CAGGTGCCAG CATCTCTGCC GGTGACTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081623.3
CDS	114..4226
Translation	MVPMETQLQSIFEEVVKTEIIEEAFPGMFMDTPEDEKTKLISCLAAFRQFWSGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNGDSTLQIPIPHSLKLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRVPLPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSHRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTDENEIITQFSVQGFPPLFLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQSDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLKDNRPQGWCLSDTYLKHAMNAREDNPWVPEDSYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVNVISSSSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAVPPQALNSGSPAPQSNQVPASLPVTQ

Target ORF information:

RefSeq Version	NM_027347.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 23 (Med23), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027347.3

ORF Insert Sequence:

ATGGTACCGA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT 
ATAGAAGAGG CCTTCCCAGG AATGTTTATG GATACCCCCG AGGATGAAAA AACAAAACTA 
ATTAGCTGCT TGGCGGCCTT CCGGCAGTTT TGGAGTGGAC TTTCTCAGGA GTCTCATGAA 
CAGTGTGTTC AGTGGATTGT TAAATTTATT CATGGCCAAC ACAGCCCCAA AAGAATTTCT 
TTCCTCTATG ACTGCTTAGC AATGGCAGTT GAGACTGGTC TCCTTCCACC CAGGATGGTT 
TGTGAATCTT TGATAAACTC TGACTCTCTT GAATGGGAAA GAACACAGCT TTGGGCCTTA 
ACATTTAAGC TGGTTCGGAA AATCATTGGA GGAGTGGATT ACAAGGGTGT TCGAGACCTT 
CTCAAAGCGA TTTTGGAGAA AATCTTGACA ATCCCAAATA CAGTGAGCTC TGCTGTTGTC 
CAGCAGCTTC TGGCAGCAAG AGAGGTTATA GCTTATATCT TAGAACGAAA TGCCTGCCTA 
TTGCCGGCCT ACTTTGCAGT CACAGAGATC AGAAAACTAT ATCCTGAGGG AAAACTTCCA 
CACTGGTTAC TAGGAAACTT GGTATCAGAC TTTGTGGATA CCTTCAGGCC CACAGCAAGG 
ATTAACTCCA TTTGTGGCCG ATGCAGTCTT CTGCCAGTTG TGAACAACTC GGGAGCCATC 
TGTAACTCAT GGAAACTGGA CCCTGCAACT CTGCGATTTC CTTTGAAAGG CCTTTTGCCA 
TATGATAAGG ATCTGTTTGA ACCGCAGACT GCTTTGTTGA GATATGTACT GGAGCAACCT 
TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGCACAA GCAGCGCTGC 
CCTGTGCTGG AGGACCAGTT GGTGGACCTG GTGGTGTACG CCATGGAGAG GTCAGAGACC 
GAGGAGAAGT TTGACGATGG GGGAACCAGC CAGCTCCTGT GGCAGCACCT TTCCAGCCAG 
CTCATTTTCT TTGTGCTTTT CCAGTTTGCC AGTTTCCCAC ATATGGTCCT CTCTCTCCAC 
CAGAAGTTAG CAGGGCGAGG ACTGATTAAA GGGCGAGACC ATCTGATGTG GGTGCTGCTG 
CAGTTCATTT CTGGAAGTAT TCAGAAAAAT GCCCTCGCTG ACTTCCTCCC TGTCATGAAG 
CTCTTTGACC TGCTGTACCC GGAGAAGGAG TGTATCCCAG TTCCTGATAT TAACAAACCC 
CAGTCAACGC ACGCCTTTGC AATGACTTGT ATTTGGATTC ATCTTAATAG AAAAGCTCAA 
AATGGCGACT CCACGCTCCA GATCCCAATA CCCCATTCCC TGAAGCTGCA CCATGAGTTC 
CTCCAGCAGA GTCTGAGAAA TAAAAGCTTA CAGATGAATG ACTATAAGAT TGCCCTGCTA 
TGTAACGCGT ATTCCACAAA CTCAGAGTGT TTTACGTTAC CTATGGGAGC TCTGGTAGAA 
ACTATTTATG GAAATGGAAT TATGAGAGTG CCTCTCCCTG GAACGAGCTG TTTGGCCTCA 
GCATCAGTGA CTCCCTTACC GATGAACCTC CTGGATTCAC TGACGGTGCA TGCCAAGATG 
AGCCTTATTC ACAGCATTGC AACCAGAGTG ATAAAACTGG CTCATACGAA ATCCAGTGTT 
GCGCTGGCTC CAGCCCTAGT GGAAACTTAC AGCCGTTTAC TGGTCTACAT GGAAATTGAG 
TCTTTGGGCA TCAAAGGATT TATCAGTCAG CTCCTGCCCA CTGTCTTCAA GTCCCACGCC 
TGGGGCATCC TGCACACACT GCTGGAGATG TTCAGCCACC GAATGCACCA CATTCAGCCC 
CACTACCGAG TTCAGCTCCT GAGCCATCTC CACACACTGG CTGCAGTCGC ACAGACCAAC 
CAGAACCAGC TCCATCTGTG TGTGGAGAGC ACTGCACTGA GGCTCATCAC AGCCCTGGGA 
AGCTCAGAGG TCCAGCCGCA GTTCACGCGT TTCCTCAATG ATCCCAAAAC AGTGCTGTCC 
GCCGAATCTG AAGAGCTGAA CCGAGCCTTG ATCCTGACCT TAGCCAGAGC AACCCACGTC 
ACAGATTTTT TTACAGGATC TGATTCAATA CAGGGAACTT GGTGTAAAGA TATCCTTCAG 
ACCATCATGA ACTTTACTCC TCATAACTGG GCTTCACATA CCCTTAGCTG TTTTCCAGCC 
CCTCTGCAGG CTTTCTTCAA GCAAAATAAC GTGCCCCAGG AAAGCCGTTT TAATCTGAAG 
AAGAATGTGG AAGAAGAGTA CAGGAAGTGG AAGTCAATGA CTGATGAAAA CGAGATCATC 
ACCCAGTTCT CTGTGCAGGG CTTCCCTCCA CTCTTCCTCT GTCTCCTCTG GAAGATGCTC 
CTGGAGACGG ATCATATTAG TCAGATCGGC TACAAGGTCC TGGAGAGGAT TGGGGCCAGG 
GCCCTGGTGG CCCATGTGAG AACATTTGCA GATTTCCTGG TGTATGAGTT CTCTACGTCA 
GCAGGAGGTC AGCAACTCAA TAAGTGCATT GAAATTCTCA ACGACATGGT GTGGAAGTAC 
AACATTGTCA CGCTGGACAG ACTCATTCTC TGCCTGGCTA TGCGTAGTCA TGAAGGAAAT 
GAAGCCCAGG TTTGTTATTT CATAATTCAG TTGCTGTTGC TCAAACCAAA TGACTTCAGA 
AACCGAGTAA GTGACTTTGT GAAGGAGAAT TCCCCAGAGC ACTGGCTCCA GAGTGACTGG 
CACACCAAGC ACATGAGCTA CCACAAGAAA TATCCCGAGA AGCTGTATTT TGAAGGCCTG 
GCAGAGCAGG TTGACCCTCC TGTACCAATC CAGTCCCCTT ATCTGCCCAT CTACTTTGGA 
AATGTGTGTC TCCGGTTCCT TCCAGTATTT GATATAGTAA TCCACAGGTT TTTAGAGTTG 
CTTCCAGTGT CCAAATCACT AGAGACTCTC CTGGATCACC TAGGGGGCTT ATATAAATTC 
CATGACCGGC CAGTGACTTA TCTGTATAAT ACTTTGCACT ATTATGAAAT GTGCCTGAGA 
AACCGTGACC ATCTCAAACG AAAACTTGTC CATGCAATCA TTGGCTCCCT CAAAGATAAC 
CGGCCACAGG GCTGGTGTCT CAGTGATACC TACCTGAAGC ATGCTATGAA TGCACGGGAG 
GACAACCCCT GGGTTCCGGA GGACTCCTAC TACTGCAAGC TGATTGGCCG ACTGGTAGAC 
ACCATGGCGG GCAAGTCTCC AGGACCCTTC CCAAACTGTG ACTGGAGATT CAATGAGTTT 
CCCAACCCCG CTGCCCACGC TCTGCATGTG ACGTGTGTGG AACTCATGGC CTTGGCGGTT 
CCAGGCAAAG ATGTGGGGAA TGCCCTTCTC AATGTCGTCC TGAAAAGCCA GCCCTTAGTG 
CCAAGGGAGA ACATTACAGC GTGGATGAAT GCAATTGGTC TGATCATCAC GGCCCTCCCA 
GAGCCGTACT GGATTGTGCT TCATGACCGA ATTGTGAATG TGATCAGCAG TTCTAGCCTG 
ACGTCAGAGA CAGAGTGGGT CGGTTACCCG TTCCGCCTCT TCGACTTCAC CGCTTGTCAC 
CAGTCCTACT CGGAGATGAG CTGCAGCTAT ACGTTAGCTC TAGCCCATGC CGTGTGGCAC 
CACTCCAGCA TCGGGCAGCT CTCGCTTATC CCAAAGTTTC TCACTGAGGC GCTTCTCCCG 
GTGGTGAAGA CTGAGTTCCA GTTGCTCTAC GTGTACCATC TTGTGGGGCC ATTTCTACAG 
AGGTTCCAAC AGGAGAGAAC CCGGTGCATG ATTGAGATTG GCGTGGCATT TTATGACATG 
CTGTTGAATG TAGACCAGTG CAGCACGCAC TTAAATTACA TGGACCCCAT CTGCGACTTC 
CTGTATCATA TGAAGTATAT GTTTACTGGT GACAGTGTGA AAGAGCAAGT AGAGAAGATT 
ATCTGTAATT TAAAGCCGGC TTTAAAGCTT CGTCTTCGAT TCATCACGCA CATTAGCAAG 
ATGGAGCCGG CAGTGCCACC CCAGGCCCTC AACAGCGGGT CTCCAGCACC TCAGTCTAAC 
CAGGTGCCAG CATCTCTGCC GGTGACTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Med23 ( NM_001166416.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001159888.1 Mus musculus mediator complex subunit 23 (Med23), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu10419
Clone ID Related Accession (Same CDS sequence)	NM_001166416.1
Accession Version	NM_001166416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 23 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ100894.1, AK129312.1, AC159502.12 and BQ885176.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGTACCGA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT 
ATAGAAGAGG CCTTCCCAGG AATGTTTATG GATACCCCCG AGGATGAAAA AACAAAACTA 
ATTAGCTGCT TGGCGGCCTT CCGGCAGTTT TGGAGTGGAC TTTCTCAGGA GTCTCATGAA 
CAGTGTGTTC AGTGGATTGT TAAATTTATT CATGGCCAAC ACAGCCCCAA AAGAATTTCT 
TTCCTCTATG ACTGCTTAGC AATGGCAGTT GAGACTGGTC TCCTTCCACC CAGGATGGTT 
TGTGAATCTT TGATAAACTC TGACTCTCTT GAATGGGAAA GAACACAGCT TTGGGCCTTA 
ACATTTAAGC TGGTTCGGAA AATCATTGGA GGAGTGGATT ACAAGGGTGT TCGAGACCTT 
CTCAAAGCGA TTTTGGAGAA AATCTTGACA ATCCCAAATA CAGTGAGCTC TGCTGTTGTC 
CAGCAGCTTC TGGCAGCAAG AGAGGTTATA GCTTATATCT TAGAACGAAA TGCCTGCCTA 
TTGCCGGCCT ACTTTGCAGT CACAGAGATC AGAAAACTAT ATCCTGAGGG AAAACTTCCA 
CACTGGTTAC TAGGAAACTT GGTATCAGAC TTTGTGGATA CCTTCAGGCC CACAGCAAGG 
ATTAACTCCA TTTGTGGCCG ATGCAGTCTT CTGCCAGTTG TGAACAACTC GGGAGCCATC 
TGTAACTCAT GGAAACTGGA CCCTGCAACT CTGCGATTTC CTTTGAAAGG CCTTTTGCCA 
TATGATAAGG ATCTGTTTGA ACCGCAGACT GCTTTGTTGA GATATGTACT GGAGCAACCT 
TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGACCTT GAACATTGCT 
CAGCACAAGC AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGACCTGGT GGTGTACGCC 
ATGGAGAGGT CAGAGACCGA GGAGAAGTTT GACGATGGGG GAACCAGCCA GCTCCTGTGG 
CAGCACCTTT CCAGCCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCCAG TTTCCCACAT 
ATGGTCCTCT CTCTCCACCA GAAGTTAGCA GGGCGAGGAC TGATTAAAGG GCGAGACCAT 
CTGATGTGGG TGCTGCTGCA GTTCATTTCT GGAAGTATTC AGAAAAATGC CCTCGCTGAC 
TTCCTCCCTG TCATGAAGCT CTTTGACCTG CTGTACCCGG AGAAGGAGTG TATCCCAGTT 
CCTGATATTA ACAAACCCCA GTCAACGCAC GCCTTTGCAA TGACTTGTAT TTGGATTCAT 
CTTAATAGAA AAGCTCAAAA TGGCGACTCC ACGCTCCAGA TCCCAATACC CCATTCCCTG 
AAGCTGCACC ATGAGTTCCT CCAGCAGAGT CTGAGAAATA AAAGCTTACA GATGAATGAC 
TATAAGATTG CCCTGCTATG TAACGCGTAT TCCACAAACT CAGAGTGTTT TACGTTACCT 
ATGGGAGCTC TGGTAGAAAC TATTTATGGA AATGGAATTA TGAGAGTGCC TCTCCCTGGA 
ACGAGCTGTT TGGCCTCAGC ATCAGTGACT CCCTTACCGA TGAACCTCCT GGATTCACTG 
ACGGTGCATG CCAAGATGAG CCTTATTCAC AGCATTGCAA CCAGAGTGAT AAAACTGGCT 
CATACGAAAT CCAGTGTTGC GCTGGCTCCA GCCCTAGTGG AAACTTACAG CCGTTTACTG 
GTCTACATGG AAATTGAGTC TTTGGGCATC AAAGGATTTA TCAGTCAGCT CCTGCCCACT 
GTCTTCAAGT CCCACGCCTG GGGCATCCTG CACACACTGC TGGAGATGTT CAGCCACCGA 
ATGCACCACA TTCAGCCCCA CTACCGAGTT CAGCTCCTGA GCCATCTCCA CACACTGGCT 
GCAGTCGCAC AGACCAACCA GAACCAGCTC CATCTGTGTG TGGAGAGCAC TGCACTGAGG 
CTCATCACAG CCCTGGGAAG CTCAGAGGTC CAGCCGCAGT TCACGCGTTT CCTCAATGAT 
CCCAAAACAG TGCTGTCCGC CGAATCTGAA GAGCTGAACC GAGCCTTGAT CCTGACCTTA 
GCCAGAGCAA CCCACGTCAC AGATTTTTTT ACAGGATCTG ATTCAATACA GGGAACTTGG 
TGTAAAGATA TCCTTCAGAC CATCATGAAC TTTACTCCTC ATAACTGGGC TTCACATACC 
CTTAGCTGTT TTCCAGCCCC TCTGCAGGCT TTCTTCAAGC AAAATAACGT GCCCCAGGAA 
AGCCGTTTTA ATCTGAAGAA GAATGTGGAA GAAGAGTACA GGAAGTGGAA GTCAATGACT 
GATGAAAACG AGATCATCAC CCAGTTCTCT GTGCAGGGCT TCCCTCCACT CTTCCTCTGT 
CTCCTCTGGA AGATGCTCCT GGAGACGGAT CATATTAGTC AGATCGGCTA CAAGGTCCTG 
GAGAGGATTG GGGCCAGGGC CCTGGTGGCC CATGTGAGAA CATTTGCAGA TTTCCTGGTG 
TATGAGTTCT CTACGTCAGC AGGAGGTCAG CAACTCAATA AGTGCATTGA AATTCTCAAC 
GACATGGTGT GGAAGTACAA CATTGTCACG CTGGACAGAC TCATTCTCTG CCTGGCTATG 
CGTAGTCATG AAGGAAATGA AGCCCAGGTT TGTTATTTCA TAATTCAGTT GCTGTTGCTC 
AAACCAAATG ACTTCAGAAA CCGAGTAAGT GACTTTGTGA AGGAGAATTC CCCAGAGCAC 
TGGCTCCAGA GTGACTGGCA CACCAAGCAC ATGAGCTACC ACAAGAAATA TCCCGAGAAG 
CTGTATTTTG AAGGCCTGGC AGAGCAGGTT GACCCTCCTG TACCAATCCA GTCCCCTTAT 
CTGCCCATCT ACTTTGGAAA TGTGTGTCTC CGGTTCCTTC CAGTATTTGA TATAGTAATC 
CACAGGTTTT TAGAGTTGCT TCCAGTGTCC AAATCACTAG AGACTCTCCT GGATCACCTA 
GGGGGCTTAT ATAAATTCCA TGACCGGCCA GTGACTTATC TGTATAATAC TTTGCACTAT 
TATGAAATGT GCCTGAGAAA CCGTGACCAT CTCAAACGAA AACTTGTCCA TGCAATCATT 
GGCTCCCTCA AAGATAACCG GCCACAGGGC TGGTGTCTCA GTGATACCTA CCTGAAGCAT 
GCTATGAATG CACGGGAGGA CAACCCCTGG GTTCCGGAGG ACTCCTACTA CTGCAAGCTG 
ATTGGCCGAC TGGTAGACAC CATGGCGGGC AAGTCTCCAG GACCCTTCCC AAACTGTGAC 
TGGAGATTCA ATGAGTTTCC CAACCCCGCT GCCCACGCTC TGCATGTGAC GTGTGTGGAA 
CTCATGGCCT TGGCGGTTCC AGGCAAAGAT GTGGGGAATG CCCTTCTCAA TGTCGTCCTG 
AAAAGCCAGC CCTTAGTGCC AAGGGAGAAC ATTACAGCGT GGATGAATGC AATTGGTCTG 
ATCATCACGG CCCTCCCAGA GCCGTACTGG ATTGTGCTTC ATGACCGAAT TGTGAATGTG 
ATCAGCAGTT CTAGCCTGAC GTCAGAGACA GAGTGGGTCG GTTACCCGTT CCGCCTCTTC 
GACTTCACCG CTTGTCACCA GTCCTACTCG GAGATGAGCT GCAGCTATAC GTTAGCTCTA 
GCCCATGCCG TGTGGCACCA CTCCAGCATC GGGCAGCTCT CGCTTATCCC AAAGTTTCTC 
ACTGAGGCGC TTCTCCCGGT GGTGAAGACT GAGTTCCAGT TGCTCTACGT GTACCATCTT 
GTGGGGCCAT TTCTACAGAG GTTCCAACAG GAGAGAACCC GGTGCATGAT TGAGATTGGC 
GTGGCATTTT ATGACATGCT GTTGAATGTA GACCAGTGCA GCACGCACTT AAATTACATG 
GACCCCATCT GCGACTTCCT GTATCATATG AAGTATATGT TTACTGGTGA CAGTGTGAAA 
GAGCAAGTAG AGAAGATTAT CTGTAATTTA AAGCCGGCTT TAAAGCTTCG TCTTCGATTC 
ATCACGCACA TTAGCAAGAT GGAGCCGGCA GTGCCACCCC AGGCCCTCAA CAGCGGGTCT 
CCAGCACCTC AGTCTAACCA GGTGCCAGCA TCTCTGCCGG TGACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159888.1
CDS	114..4244
Translation	MVPMETQLQSIFEEVVKTEIIEEAFPGMFMDTPEDEKTKLISCLAAFRQFWSGLSQESHEQCVQWIVKFIHGQHSPKRISFLYDCLAMAVETGLLPPRMVCESLINSDSLEWERTQLWALTFKLVRKIIGGVDYKGVRDLLKAILEKILTIPNTVSSAVVQQLLAAREVIAYILERNACLLPAYFAVTEIRKLYPEGKLPHWLLGNLVSDFVDTFRPTARINSICGRCSLLPVVNNSGAICNSWKLDPATLRFPLKGLLPYDKDLFEPQTALLRYVLEQPYSRDMVCNMLGLNKQTLNIAQHKQRCPVLEDQLVDLVVYAMERSETEEKFDDGGTSQLLWQHLSSQLIFFVLFQFASFPHMVLSLHQKLAGRGLIKGRDHLMWVLLQFISGSIQKNALADFLPVMKLFDLLYPEKECIPVPDINKPQSTHAFAMTCIWIHLNRKAQNGDSTLQIPIPHSLKLHHEFLQQSLRNKSLQMNDYKIALLCNAYSTNSECFTLPMGALVETIYGNGIMRVPLPGTSCLASASVTPLPMNLLDSLTVHAKMSLIHSIATRVIKLAHTKSSVALAPALVETYSRLLVYMEIESLGIKGFISQLLPTVFKSHAWGILHTLLEMFSHRMHHIQPHYRVQLLSHLHTLAAVAQTNQNQLHLCVESTALRLITALGSSEVQPQFTRFLNDPKTVLSAESEELNRALILTLARATHVTDFFTGSDSIQGTWCKDILQTIMNFTPHNWASHTLSCFPAPLQAFFKQNNVPQESRFNLKKNVEEEYRKWKSMTDENEIITQFSVQGFPPLFLCLLWKMLLETDHISQIGYKVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQSDWHTKHMSYHKKYPEKLYFEGLAEQVDPPVPIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMCLRNRDHLKRKLVHAIIGSLKDNRPQGWCLSDTYLKHAMNAREDNPWVPEDSYYCKLIGRLVDTMAGKSPGPFPNCDWRFNEFPNPAAHALHVTCVELMALAVPGKDVGNALLNVVLKSQPLVPRENITAWMNAIGLIITALPEPYWIVLHDRIVNVISSSSLTSETEWVGYPFRLFDFTACHQSYSEMSCSYTLALAHAVWHHSSIGQLSLIPKFLTEALLPVVKTEFQLLYVYHLVGPFLQRFQQERTRCMIEIGVAFYDMLLNVDQCSTHLNYMDPICDFLYHMKYMFTGDSVKEQVEKIICNLKPALKLRLRFITHISKMEPAVPPQALNSGSPAPQSNQVPASLPVTQ

Target ORF information:

RefSeq Version	NM_001166416.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 23 (Med23), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166416.1

ORF Insert Sequence:

ATGGTACCGA TGGAGACGCA ACTGCAGAGC ATTTTCGAGG AGGTGGTGAA AACAGAAATT 
ATAGAAGAGG CCTTCCCAGG AATGTTTATG GATACCCCCG AGGATGAAAA AACAAAACTA 
ATTAGCTGCT TGGCGGCCTT CCGGCAGTTT TGGAGTGGAC TTTCTCAGGA GTCTCATGAA 
CAGTGTGTTC AGTGGATTGT TAAATTTATT CATGGCCAAC ACAGCCCCAA AAGAATTTCT 
TTCCTCTATG ACTGCTTAGC AATGGCAGTT GAGACTGGTC TCCTTCCACC CAGGATGGTT 
TGTGAATCTT TGATAAACTC TGACTCTCTT GAATGGGAAA GAACACAGCT TTGGGCCTTA 
ACATTTAAGC TGGTTCGGAA AATCATTGGA GGAGTGGATT ACAAGGGTGT TCGAGACCTT 
CTCAAAGCGA TTTTGGAGAA AATCTTGACA ATCCCAAATA CAGTGAGCTC TGCTGTTGTC 
CAGCAGCTTC TGGCAGCAAG AGAGGTTATA GCTTATATCT TAGAACGAAA TGCCTGCCTA 
TTGCCGGCCT ACTTTGCAGT CACAGAGATC AGAAAACTAT ATCCTGAGGG AAAACTTCCA 
CACTGGTTAC TAGGAAACTT GGTATCAGAC TTTGTGGATA CCTTCAGGCC CACAGCAAGG 
ATTAACTCCA TTTGTGGCCG ATGCAGTCTT CTGCCAGTTG TGAACAACTC GGGAGCCATC 
TGTAACTCAT GGAAACTGGA CCCTGCAACT CTGCGATTTC CTTTGAAAGG CCTTTTGCCA 
TATGATAAGG ATCTGTTTGA ACCGCAGACT GCTTTGTTGA GATATGTACT GGAGCAACCT 
TATTCCAGGG ATATGGTCTG CAATATGCTA GGTTTAAATA AGCAGACCTT GAACATTGCT 
CAGCACAAGC AGCGCTGCCC TGTGCTGGAG GACCAGTTGG TGGACCTGGT GGTGTACGCC 
ATGGAGAGGT CAGAGACCGA GGAGAAGTTT GACGATGGGG GAACCAGCCA GCTCCTGTGG 
CAGCACCTTT CCAGCCAGCT CATTTTCTTT GTGCTTTTCC AGTTTGCCAG TTTCCCACAT 
ATGGTCCTCT CTCTCCACCA GAAGTTAGCA GGGCGAGGAC TGATTAAAGG GCGAGACCAT 
CTGATGTGGG TGCTGCTGCA GTTCATTTCT GGAAGTATTC AGAAAAATGC CCTCGCTGAC 
TTCCTCCCTG TCATGAAGCT CTTTGACCTG CTGTACCCGG AGAAGGAGTG TATCCCAGTT 
CCTGATATTA ACAAACCCCA GTCAACGCAC GCCTTTGCAA TGACTTGTAT TTGGATTCAT 
CTTAATAGAA AAGCTCAAAA TGGCGACTCC ACGCTCCAGA TCCCAATACC CCATTCCCTG 
AAGCTGCACC ATGAGTTCCT CCAGCAGAGT CTGAGAAATA AAAGCTTACA GATGAATGAC 
TATAAGATTG CCCTGCTATG TAACGCGTAT TCCACAAACT CAGAGTGTTT TACGTTACCT 
ATGGGAGCTC TGGTAGAAAC TATTTATGGA AATGGAATTA TGAGAGTGCC TCTCCCTGGA 
ACGAGCTGTT TGGCCTCAGC ATCAGTGACT CCCTTACCGA TGAACCTCCT GGATTCACTG 
ACGGTGCATG CCAAGATGAG CCTTATTCAC AGCATTGCAA CCAGAGTGAT AAAACTGGCT 
CATACGAAAT CCAGTGTTGC GCTGGCTCCA GCCCTAGTGG AAACTTACAG CCGTTTACTG 
GTCTACATGG AAATTGAGTC TTTGGGCATC AAAGGATTTA TCAGTCAGCT CCTGCCCACT 
GTCTTCAAGT CCCACGCCTG GGGCATCCTG CACACACTGC TGGAGATGTT CAGCCACCGA 
ATGCACCACA TTCAGCCCCA CTACCGAGTT CAGCTCCTGA GCCATCTCCA CACACTGGCT 
GCAGTCGCAC AGACCAACCA GAACCAGCTC CATCTGTGTG TGGAGAGCAC TGCACTGAGG 
CTCATCACAG CCCTGGGAAG CTCAGAGGTC CAGCCGCAGT TCACGCGTTT CCTCAATGAT 
CCCAAAACAG TGCTGTCCGC CGAATCTGAA GAGCTGAACC GAGCCTTGAT CCTGACCTTA 
GCCAGAGCAA CCCACGTCAC AGATTTTTTT ACAGGATCTG ATTCAATACA GGGAACTTGG 
TGTAAAGATA TCCTTCAGAC CATCATGAAC TTTACTCCTC ATAACTGGGC TTCACATACC 
CTTAGCTGTT TTCCAGCCCC TCTGCAGGCT TTCTTCAAGC AAAATAACGT GCCCCAGGAA 
AGCCGTTTTA ATCTGAAGAA GAATGTGGAA GAAGAGTACA GGAAGTGGAA GTCAATGACT 
GATGAAAACG AGATCATCAC CCAGTTCTCT GTGCAGGGCT TCCCTCCACT CTTCCTCTGT 
CTCCTCTGGA AGATGCTCCT GGAGACGGAT CATATTAGTC AGATCGGCTA CAAGGTCCTG 
GAGAGGATTG GGGCCAGGGC CCTGGTGGCC CATGTGAGAA CATTTGCAGA TTTCCTGGTG 
TATGAGTTCT CTACGTCAGC AGGAGGTCAG CAACTCAATA AGTGCATTGA AATTCTCAAC 
GACATGGTGT GGAAGTACAA CATTGTCACG CTGGACAGAC TCATTCTCTG CCTGGCTATG 
CGTAGTCATG AAGGAAATGA AGCCCAGGTT TGTTATTTCA TAATTCAGTT GCTGTTGCTC 
AAACCAAATG ACTTCAGAAA CCGAGTAAGT GACTTTGTGA AGGAGAATTC CCCAGAGCAC 
TGGCTCCAGA GTGACTGGCA CACCAAGCAC ATGAGCTACC ACAAGAAATA TCCCGAGAAG 
CTGTATTTTG AAGGCCTGGC AGAGCAGGTT GACCCTCCTG TACCAATCCA GTCCCCTTAT 
CTGCCCATCT ACTTTGGAAA TGTGTGTCTC CGGTTCCTTC CAGTATTTGA TATAGTAATC 
CACAGGTTTT TAGAGTTGCT TCCAGTGTCC AAATCACTAG AGACTCTCCT GGATCACCTA 
GGGGGCTTAT ATAAATTCCA TGACCGGCCA GTGACTTATC TGTATAATAC TTTGCACTAT 
TATGAAATGT GCCTGAGAAA CCGTGACCAT CTCAAACGAA AACTTGTCCA TGCAATCATT 
GGCTCCCTCA AAGATAACCG GCCACAGGGC TGGTGTCTCA GTGATACCTA CCTGAAGCAT 
GCTATGAATG CACGGGAGGA CAACCCCTGG GTTCCGGAGG ACTCCTACTA CTGCAAGCTG 
ATTGGCCGAC TGGTAGACAC CATGGCGGGC AAGTCTCCAG GACCCTTCCC AAACTGTGAC 
TGGAGATTCA ATGAGTTTCC CAACCCCGCT GCCCACGCTC TGCATGTGAC GTGTGTGGAA 
CTCATGGCCT TGGCGGTTCC AGGCAAAGAT GTGGGGAATG CCCTTCTCAA TGTCGTCCTG 
AAAAGCCAGC CCTTAGTGCC AAGGGAGAAC ATTACAGCGT GGATGAATGC AATTGGTCTG 
ATCATCACGG CCCTCCCAGA GCCGTACTGG ATTGTGCTTC ATGACCGAAT TGTGAATGTG 
ATCAGCAGTT CTAGCCTGAC GTCAGAGACA GAGTGGGTCG GTTACCCGTT CCGCCTCTTC 
GACTTCACCG CTTGTCACCA GTCCTACTCG GAGATGAGCT GCAGCTATAC GTTAGCTCTA 
GCCCATGCCG TGTGGCACCA CTCCAGCATC GGGCAGCTCT CGCTTATCCC AAAGTTTCTC 
ACTGAGGCGC TTCTCCCGGT GGTGAAGACT GAGTTCCAGT TGCTCTACGT GTACCATCTT 
GTGGGGCCAT TTCTACAGAG GTTCCAACAG GAGAGAACCC GGTGCATGAT TGAGATTGGC 
GTGGCATTTT ATGACATGCT GTTGAATGTA GACCAGTGCA GCACGCACTT AAATTACATG 
GACCCCATCT GCGACTTCCT GTATCATATG AAGTATATGT TTACTGGTGA CAGTGTGAAA 
GAGCAAGTAG AGAAGATTAT CTGTAATTTA AAGCCGGCTT TAAAGCTTCG TCTTCGATTC 
ATCACGCACA TTAGCAAGAT GGAGCCGGCA GTGCCACCCC AGGCCCTCAA CAGCGGGTCT 
CCAGCACCTC AGTCTAACCA GGTGCCAGCA TCTCTGCCGG TGACTCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.
Cell stem cell19(6)784-799(2016 Dec)
Aranda-Orgilles B,Salda?a-Meyer R,Wang E,Trompouki E,Fassl A,Lau S,Mullenders J,Rocha PP,Raviram R,Guillamot M,S?nchez-D?az M,Wang K,Kayembe C,Zhang N,Amoasii L,Choudhuri A,Skok JA,Schober M,Reinberg D,Sicinski P,Schrewe H,Tsirigos A,Zon LI,Aifantis I

Mediator MED23 cooperates with RUNX2 to drive osteoblast differentiation and bone development.
Nature communications711149(2016 Apr)
Liu Z,Yao X,Yan G,Xu Y,Yan J,Zou W,Wang G