Ttl cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ttl
Entrez Gene ID	69737
Full Name	tubulin tyrosine ligase
Synonyms	2410003M22Rik,2700049H19Rik,AI848570
General protein information	Preferred Names tubulin tyrosine ligase Names tubulin--tyrosine ligase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_027192.2	NP_081468.1	tubulin--tyrosine ligase

Pathways from BioSystems

Homologies

	TTL	XP_001087668.1
Mus musculus (house mouse)	Ttl	NP_081468.1
Rattus norvegicus (Norway rat)	Ttl	NP_612545.1
Xenopus tropicalis (tropical clawed frog)	ttl	NP_001107149.1
Canis lupus familiaris (dog)	TTL	XP_540180.2
Gallus gallus (chicken)	TTL	NP_001186321.1
Pan troglodytes (chimpanzee)	TTL	XP_525864.3
Homo sapiens (human)	TTL	NP_714923.1
Bos taurus (cattle)	TTL	NP_001106704.1
Danio rerio (zebrafish)	ttl	NP_001070093.1

Gene Ontology
Bibliography

Related articles in PubMed

The GSK3-MAP1B pathway controls neurite branching and microtubule dynamics.
Barnat M, Benassy MN, Vincensini L, Soares S, Fassier C, Propst F, Andrieux A, von Boxberg Y, Nothias F
Molecular and cellular neurosciences729-21(2016 Apr)

Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated ?-Tubulin in Injured Axons Is Required for Retrograde Injury Signaling and Axon Regeneration.
Song W, Cho Y, Watt D, Cavalli V
The Journal of biological chemistry290(23)14765-75(2015 Jun)

Cap-Gly proteins at microtubule plus ends: is EB1 detyrosination involved?
Bosson A, Soleilhac JM, Valiron O, Job D, Andrieux A, Moutin MJ
PloS one7(3)e33490(2012)

Motor-dependent microtubule disassembly driven by tubulin tyrosination.
Peris L, Wagenbach M, Lafanech?re L, Brocard J, Moore AT, Kozielski F, Job D, Wordeman L, Andrieux A
The Journal of cell biology185(7)1159-66(2009 Jun)

Tubulin tyrosination is required for the proper organization and pathfinding of the growth cone.
Marcos S, Moreau J, Backer S, Job D, Andrieux A, Bloch-Gallego E
PloS one4(4)e5405(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tubulin-tyrosine ligase (TTL) is required to increase the levels of tyrosinated alpha-tubulin at the axon injury site and plays an important role in injury signaling.
Title: Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated ?-Tubulin in Injured Axons Is Required for Retrograde Injury Signaling and Axon Regeneration.

Our results show that MAP1B abd tubulin-tyrosine ligase indeed interact and that the interaction is not affected by MAP1B phosphorylation.
Title: Microtubule-associated protein 1B interaction with tubulin tyrosine ligase contributes to the control of microtubule tyrosination.

The following Ttl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ttl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03836	NM_027192.2 Latest version!	Mus musculus tubulin tyrosine ligase (Ttl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ttl ( NM_027192.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081468.1 Mus musculus tubulin tyrosine ligase (Ttl), mRNA.

Price & Availability↑

CloneID	OMu03836
Clone ID Related Accession (Same CDS sequence)	NM_027192.2
Accession Version	NM_027192.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1134bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	tubulin--tyrosine ligase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL833780.6. On Aug 15, 2009 this sequence version replaced NM_027192.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC024414.1, SRR1660815.186830.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACACCT TCGTGGTGCG CGACGAGAAT AGCAGCGTCT ACGCCGAGGT CTCCCGGCTG 
CTGCTGGCCA CCGGCTACTG GAAGAGGCTA CGGCGAGACA ACCCCAGGTT CAACCTGATG 
CTGGGGGAGA GGAACCGACT GCCCTTCGGG AGACTAGGTC ACGAGCCTGG GCTGGCACAG 
CTGGTGAATT ACTACCGGGG GGCGGACAAA CTGTGCCGCA AAGCTTCCTT AGTAAAGCTA 
GTCAAGACCA GTCCGGAACT GTCTGAGTCC TGCTCATGGT TCCCCGAGTC GTATGTGATT 
TATCCAACTA ACCTCAAGAC GCCAGTCGCT CCGGCGCAGA ATGGCATCCA ACTTCCGGTC 
AGTAACTCCA GGACAGATGA GAGAGAATTC TTCTTGGCTT CTTATAACAG AAAGAAAGAG 
GACGGGGAGG GCAACGTTTG GATTGCAAAG TCATCAGCTG GTGCCAAAGG TGAGGGCATC 
CTCATCTCCT CAGAGGCATC GGAGCTTCTG GATTTCATCG ACAGCCAGGG CCAAGTGCAC 
GTGATCCAGA AATACCTCGA GCGCCCTCTG CTCCTCGAGC CCGGCCACCG CAAGTTTGAC 
ATTCGAAGCT GGGTCCTGGT TGACCATCAG TATAACATCT ACCTCTATAG AGAGGGTGTG 
CTTCGAACTG CTTCAGAACC ATATCATGTT GATAATTTCC AAGATAAGAC CTGCCATCTG 
ACCAATCACT GCATTCAGAA AGAGTACTCA AAGAACTATG GGAAATACGA AGAAGGGAAT 
GAAATGTTCT TCGAAGAGTT CAATCAGTAC CTAACCAGCG CTCTGAACAT CACCTTAGAA 
AGTAGCATTT TGCTGCAAAT CAAACACATA ATAAGGAGCT GCCTCATGAG TGTGGAGCCC 
GCCATCAGCA CCAAACACCT CCCTTACCAG AGCTTCCAGC TCCTCGGCTT TGACTTCATG 
GTGGACGAGG AGCTGAAGGT CTGGCTCATT GAAGTCAACG GCGCCCCAGC GTGTGCCCAG 
AAACTCTATG CAGAACTGTG CCAGGGCATC GTGGACATAG CCATATCCAG TGTCTTCCCA 
CCCCCGGACA CGGAGCAGGT GCCGCAGCAG CCGGCTGCGT TCGTCAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081468.1
CDS	201..1334
Translation	MYTFVVRDENSSVYAEVSRLLLATGYWKRLRRDNPRFNLMLGERNRLPFGRLGHEPGLAQLVNYYRGADKLCRKASLVKLVKTSPELSESCSWFPESYVIYPTNLKTPVAPAQNGIQLPVSNSRTDEREFFLASYNRKKEDGEGNVWIAKSSAGAKGEGILISSEASELLDFIDSQGQVHVIQKYLERPLLLEPGHRKFDIRSWVLVDHQYNIYLYREGVLRTASEPYHVDNFQDKTCHLTNHCIQKEYSKNYGKYEEGNEMFFEEFNQYLTSALNITLESSILLQIKHIIRSCLMSVEPAISTKHLPYQSFQLLGFDFMVDEELKVWLIEVNGAPACAQKLYAELCQGIVDIAISSVFPPPDTEQVPQQPAAFVKL

Target ORF information:

RefSeq Version	NM_027192.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus tubulin tyrosine ligase (Ttl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027192.2

ORF Insert Sequence:

ATGTACACCT TCGTGGTGCG CGACGAGAAT AGCAGCGTCT ACGCCGAGGT CTCCCGGCTG 
CTGCTGGCCA CCGGCTACTG GAAGAGGCTA CGGCGAGACA ACCCCAGGTT CAACCTGATG 
CTGGGGGAGA GGAACCGACT GCCCTTCGGG AGACTAGGTC ACGAGCCTGG GCTGGCACAG 
CTGGTGAATT ACTACCGGGG GGCGGACAAA CTGTGCCGCA AAGCTTCCTT AGTAAAGCTA 
GTCAAGACCA GTCCGGAACT GTCTGAGTCC TGCTCATGGT TCCCCGAGTC GTATGTGATT 
TATCCAACTA ACCTCAAGAC GCCAGTCGCT CCGGCGCAGA ATGGCATCCA ACTTCCGGTC 
AGTAACTCCA GGACAGATGA GAGAGAATTC TTCTTGGCTT CTTATAACAG AAAGAAAGAG 
GACGGGGAGG GCAACGTTTG GATTGCAAAG TCATCAGCTG GTGCCAAAGG TGAGGGCATC 
CTCATCTCCT CAGAGGCATC GGAGCTTCTG GATTTCATCG ACAGCCAGGG CCAAGTGCAC 
GTGATCCAGA AATACCTCGA GCGCCCTCTG CTCCTCGAGC CCGGCCACCG CAAGTTTGAC 
ATTCGAAGCT GGGTCCTGGT TGACCATCAG TATAACATCT ACCTCTATAG AGAGGGTGTG 
CTTCGAACTG CTTCAGAACC ATATCATGTT GATAATTTCC AAGATAAGAC CTGCCATCTG 
ACCAATCACT GCATTCAGAA AGAGTACTCA AAGAACTATG GGAAATACGA AGAAGGGAAT 
GAAATGTTCT TCGAAGAGTT CAATCAGTAC CTAACCAGCG CTCTGAACAT CACCTTAGAA 
AGTAGCATTT TGCTGCAAAT CAAACACATA ATAAGGAGCT GCCTCATGAG TGTGGAGCCC 
GCCATCAGCA CCAAACACCT CCCTTACCAG AGCTTCCAGC TCCTCGGCTT TGACTTCATG 
GTGGACGAGG AGCTGAAGGT CTGGCTCATT GAAGTCAACG GCGCCCCAGC GTGTGCCCAG 
AAACTCTATG CAGAACTGTG CCAGGGCATC GTGGACATAG CCATATCCAG TGTCTTCCCA 
CCCCCGGACA CGGAGCAGGT GCCGCAGCAG CCGGCTGCGT TCGTCAAGCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The GSK3-MAP1B pathway controls neurite branching and microtubule dynamics.
Molecular and cellular neurosciences729-21(2016 Apr)
Barnat M,Benassy MN,Vincensini L,Soares S,Fassier C,Propst F,Andrieux A,von Boxberg Y,Nothias F

Tubulin-tyrosine Ligase (TTL)-mediated Increase in Tyrosinated ?-Tubulin in Injured Axons Is Required for Retrograde Injury Signaling and Axon Regeneration.
The Journal of biological chemistry290(23)14765-75(2015 Jun)
Song W,Cho Y,Watt D,Cavalli V

Cap-Gly proteins at microtubule plus ends: is EB1 detyrosination involved?
PloS one7(3)e33490(2012)
Bosson A,Soleilhac JM,Valiron O,Job D,Andrieux A,Moutin MJ

Motor-dependent microtubule disassembly driven by tubulin tyrosination.
The Journal of cell biology185(7)1159-66(2009 Jun)
Peris L,Wagenbach M,Lafanech?re L,Brocard J,Moore AT,Kozielski F,Job D,Wordeman L,Andrieux A

Tubulin tyrosination is required for the proper organization and pathfinding of the growth cone.
PloS one4(4)e5405(2009)
Marcos S,Moreau J,Backer S,Job D,Andrieux A,Bloch-Gallego E