H2bfm cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	H2bfm
Entrez Gene ID	69389
Full Name	H2B histone family, member M
Synonyms	1700014N06Rik,H2bfw,H2bl2
General protein information	Preferred Names H2B histone family, member M Names histone variant H2bl2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X F1
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the H2B histone family. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_027067.2	NP_081343.1	histone variant H2bl2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	H2bfm	NP_081343.1
Homo sapiens (human)	H2BFM	NP_001157888.1
	LOC698930	XP_001087407.2

Gene Ontology
Bibliography

Related articles in PubMed

Birth-and-death long-term evolution promotes histone H2B variant diversification in the male germinal cell line.
Gonz?lez-Romero R, Rivera-Casas C, Ausi? J, M?ndez J, Eir?n-L?pez JM
Molecular biology and evolution27(8)1802-12(2010 Aug)

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
Govin J, Escoffier E, Rousseaux S, Kuhn L, Ferro M, Th?venon J, Catena R, Davidson I, Garin J, Khochbin S, Caron C
The Journal of cell biology176(3)283-94(2007 Jan)

Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis.
McManus EJ, Sakamoto K, Armit LJ, Ronaldson L, Shpiro N, Marquez R, Alessi DR
The EMBO journal24(8)1571-83(2005 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that in glycogen synthase kinase (GSK)3alpha/GSK3beta homozygous knockin cells, Wnt3a induces normal inactivation of GSK3.
Title: Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis.

The following H2bfm gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H2bfm cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu24699	NM_027067.2 Latest version!	Mus musculus H2B histone family, member M (H2bfm), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H2bfm ( NM_027067.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081343.1 Mus musculus H2B histone family, member M (H2bfm), mRNA.

Price & Availability↑

CloneID	OMu24699
Clone ID Related Accession (Same CDS sequence)	NM_027067.2
Accession Version	NM_027067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-25
Organism	Mus musculus(house mouse)
Product	histone variant H2bl2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK005982.1 and BC117076.1. On Apr 9, 2011 this sequence version replaced NM_027067.1. ##Evidence-Data-START## Transcript is intronless :: AK005982.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 17261847 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCTA CCACGGCTAT GGACGTTCTG GAGGAGCTAT CTTCGGATAG TTCCGAAAAA 
CAGGTACAAC CAAGAAAGCC TGAGAAAGCT AAAAGGGAAA AGGACAAACC AAAGAAAGGG 
GGGCCGGAGA AGAAGGCCAA GAAAGAGAAA CAGGAGAAAG CAAAGCCGGA GAAAAAGCCG 
AAGAAGAAGC CAGAGAAAGA GAAGCCGGAG GGAGAGAAGC TGGAGAAGAA ACCCAAGAAA 
GACAAGCGGG AGAAAGCGAA GCCGAAGAAG AAGCCCGAGC AAGAGAACCG TGAGCAAGAG 
ACTCCTGAGC AGGAGAAGCC TGAGGTGCAG CGGCGTCGCT CACTTCACCA AAGCATCAGG 
GAAGATGAGC GTAGAGCCCG TCTGATCAGA CGCCGTAAGA ACAGCTTCGC TATCTACTTT 
CCGAAGGTGC TGAAAAATAT CCACGTGGGT CTCTCCCTCT CGCAGCGGTC TGTGAACATC 
CTGGATTCAT TCGTGAAGGA TATGTTTGAA AGAATTGCAT CCGAAGCCAG CTTCTTGGCG 
CGTCAAGCCA GAAACTCTAC TATCAACTCC AGAGAGATCC AGACCGCTAT TCGACTTCTG 
CTTCCTGGCG AGCTCTGCCG GCGTGCAGTG GCTGAAGGAA CCATGGCGAT GGTCCGGTAT 
ATCTCCAACA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081343.1
CDS	40..714
Translation	MASTTAMDVLEELSSDSSEKQVQPRKPEKAKREKDKPKKGGPEKKAKKEKQEKAKPEKKPKKKPEKEKPEGEKLEKKPKKDKREKAKPKKKPEQENREQETPEQEKPEVQRRRSLHQSIREDERRARLIRRRKNSFAIYFPKVLKNIHVGLSLSQRSVNILDSFVKDMFERIASEASFLARQARNSTINSREIQTAIRLLLPGELCRRAVAEGTMAMVRYISNK

Target ORF information:

RefSeq Version	NM_027067.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus H2B histone family, member M (H2bfm), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027067.2

ORF Insert Sequence:

ATGGCGTCTA CCACGGCTAT GGACGTTCTG GAGGAGCTAT CTTCGGATAG TTCCGAAAAA 
CAGGTACAAC CAAGAAAGCC TGAGAAAGCT AAAAGGGAAA AGGACAAACC AAAGAAAGGG 
GGGCCGGAGA AGAAGGCCAA GAAAGAGAAA CAGGAGAAAG CAAAGCCGGA GAAAAAGCCG 
AAGAAGAAGC CAGAGAAAGA GAAGCCGGAG GGAGAGAAGC TGGAGAAGAA ACCCAAGAAA 
GACAAGCGGG AGAAAGCGAA GCCGAAGAAG AAGCCCGAGC AAGAGAACCG TGAGCAAGAG 
ACTCCTGAGC AGGAGAAGCC TGAGGTGCAG CGGCGTCGCT CACTTCACCA AAGCATCAGG 
GAAGATGAGC GTAGAGCCCG TCTGATCAGA CGCCGTAAGA ACAGCTTCGC TATCTACTTT 
CCGAAGGTGC TGAAAAATAT CCACGTGGGT CTCTCCCTCT CGCAGCGGTC TGTGAACATC 
CTGGATTCAT TCGTGAAGGA TATGTTTGAA AGAATTGCAT CCGAAGCCAG CTTCTTGGCG 
CGTCAAGCCA GAAACTCTAC TATCAACTCC AGAGAGATCC AGACCGCTAT TCGACTTCTG 
CTTCCTGGCG AGCTCTGCCG GCGTGCAGTG GCTGAAGGAA CCATGGCGAT GGTCCGGTAT 
ATCTCCAACA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Birth-and-death long-term evolution promotes histone H2B variant diversification in the male germinal cell line.
Molecular biology and evolution27(8)1802-12(2010 Aug)
Gonz?lez-Romero R,Rivera-Casas C,Ausi? J,M?ndez J,Eir?n-L?pez JM

Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis.
The Journal of cell biology176(3)283-94(2007 Jan)
Govin J,Escoffier E,Rousseaux S,Kuhn L,Ferro M,Th?venon J,Catena R,Davidson I,Garin J,Khochbin S,Caron C

Role that phosphorylation of GSK3 plays in insulin and Wnt signalling defined by knockin analysis.
The EMBO journal24(8)1571-83(2005 Apr)
McManus EJ,Sakamoto K,Armit LJ,Ronaldson L,Shpiro N,Marquez R,Alessi DR

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H2bfm cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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