Lmbrd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lmbrd1
Entrez Gene ID	68421
Full Name	LMBR1 domain containing 1
Synonyms	0910001K20Rik,AV347960
General protein information	Preferred Names LMBR1 domain containing 1 Names probable lysosomal cobalamin transporter LMBR1 domain-containing protein 1 protein N90b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1\|1 A5

mRNA and Protein(s)

mRNA	Protein	Name
NM_001310483.1	NP_001297412.1	probable lysosomal cobalamin transporter isoform 2
NM_026719.2	NP_080995.2	probable lysosomal cobalamin transporter isoform 1
XM_017322292.1	XP_017177781.1	probable lysosomal cobalamin transporter isoform X1
XM_030242602.1	XP_030098462.1	probable lysosomal cobalamin transporter isoform X2
XM_017322292.2	XP_017177781.1	probable lysosomal cobalamin transporter isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LMBRD1	NP_001006413.1
Mus musculus (house mouse)	Lmbrd1	NP_080995.2
Danio rerio (zebrafish)	lmbrd1	NP_001008612.1
Xenopus tropicalis (tropical clawed frog)	lmbrd1	NP_001072184.1
Homo sapiens (human)	LMBRD1	NP_060838.3
Pan troglodytes (chimpanzee)	LMBRD1	XP_001136147.1
	LMBRD1	XP_001111444.1
Canis lupus familiaris (dog)	LMBRD1	XP_003639484.1
Rattus norvegicus (Norway rat)	Lmbrd1	NP_631928.2
Magnaporthe oryzae (rice blast fungus)	MGG_00740	XP_003718260.1
Bos taurus (cattle)	LMBRD1	NP_001030264.1
Neurospora crassa	NCU02100	XP_964094.1

Gene Ontology
Bibliography

Related articles in PubMed

Single allele Lmbrd1 knockout results in cardiac hypertrophy.
Tseng LT, Lin CL, Pan KH, Tzen KY, Su MJ, Tsai CT, Li YH, Li PC, Chiang FT, Chang SC, Chang MF
Journal of the Formosan Medical Association = Taiwan yi zhi(2017 May)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Lmbrd1 expression is essential for the initiation of gastrulation.
Buers I, Pennekamp P, Nitschke Y, Lowe C, Skryabin BV, Rutsch F
Journal of cellular and molecular medicine20(8)1523-33(2016 08)

Regulation of Hematopoiesis and Methionine Homeostasis by mTORC1 Inhibitor NPRL2.
Dutchak PA, Laxman S, Estill SJ, Wang C, Wang Y, Wang Y, Bulut GB, Gao J, Huang LJ, Tu BP
Cell reports12(3)371-9(2015 Jul)

LMBD1 protein serves as a specific adaptor for insulin receptor internalization.
Tseng LT, Lin CL, Tzen KY, Chang SC, Chang MF
The Journal of biological chemistry288(45)32424-32(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the physiological function of LMBRD1 during embryogenesis, is reported.
Title: Lmbrd1 expression is essential for the initiation of gastrulation.

LMBD1 plays an imperative role in mediating and regulating the endocytosis of the IR.
Title: LMBD1 protein serves as a specific adaptor for insulin receptor internalization.

The following Lmbrd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lmbrd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu70271	NM_001310483.1 Latest version!	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu85253	XM_030242602.1 Latest version!	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OMu02883	NM_026719.2 Latest version!	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$321.30 $459.00
OMu77272	XM_017322292.2 Latest version!	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OMu77272	XM_017322292.1	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lmbrd1 ( NM_001310483.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001297412.1 Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu70271
Clone ID Related Accession (Same CDS sequence)	NM_001310483.1
Accession Version	NM_001310483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1404bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Mus musculus(house mouse)
Product	probable lysosomal cobalamin transporter isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116997.3, AK158982.1 and AC153019.8. On Jul 14, 2015 this sequence version replaced XM_006496240.1. Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1, which results in translation from a downstream AUG. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK158982.1, SRR1660811.81605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAAAATC AAAATGGCAC ATTCAAGGAC TGGGCTGACG CCAATGTCAC CGTACAGATT 
GAGAATACCG TTCTGTATGG CTACTATACT CTGTATTCTG TCATTCTCTT CTGTGTGTTC 
TTCTGGATCC CGTTTGTGTA CTTCTACTAC GAAGAGAAGG ATGAGGACGA CGCCAGCAAA 
TGCACCCAAA TTAAAACGGC GCTCAAGTAC ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG 
CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG CATCTGCCCA ACAACAACAA CTCAACAGAG 
TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC CTTGGAACTG GTCAAGGCCT GGCAGCTCTG 
TCCTTTTCCA TCAGCTCTCT GACCTTGATT GGGATGCTGG CGGCCATAAC CTACACAGCG 
TACGGCATGT CGGCATTACC TTTAAACCTA ATCAAAGGCA CTAGAAGCAC TGCCTACGAG 
CGCCTGGAGA ACACGGAGGA CATCGAGGAA GTGGAGCAGC ACATTCAGAC GATTCGATCC 
AAGAGCAAAG ATGGTCGACC TTTGCCAGCG AGGGATAGAC GTGCCTTAAA GCAATGTGAA 
GAAAGACTAA GAACGCTTAG GAAAAGAGAG CGGCACTTGG AATTCATTGA AAACAGCTGG 
TGGACGAAAT TTTGTGGTGC CCTGCGCCCC CTGAAGATCA TTTGGGGAAT ATTTTTCATC 
CTCGTTGCGT TGCTGTTTGT AATTTCTCTC TTCCTGTCAA ATTTGGATAA AGCTCTTCAT 
TCAGCAGGAA TAGATTCGGG TTTCATCATC TTCGGAACTA ACCTGAGCAA TCCACTGAAC 
ATGCTTTTGC CGTTGTTACA AACAGTGTTC CCTCTTGATT ACATTCTTAT AACAATTATT 
ATTATGTACT TTATTTTTAC TTCGATGGCA GGAATTCGAA ACATTGGCAT TTGGTTCTTT 
TGGATTAGGT TATATAAAAT CAGAAGAGGG CGAACCAGGC CTCAGGCACT CTTATTTCTC 
TGCATGATCC TCCTGCTCAT TGTCCTTCAT ACCAGCTACA TGATCTACAG TCTCGCGCCT 
CAGTACGTGA TGTACGGAAG CCAGAATTAC TTAATAGAGA GCAATATAAC TTCTGATGCC 
CATAAAGGCA ATTCAACCCT CGCTGTGCCA AAAAGATGTG ACGCAGATGC CCCTAAAGAT 
CAGTGTACTG TTACCCGAAC ATACATTTTC CTCCACAAGT TCTGGTTCTT CAGTGCTGCA 
TACTATTTTG GTAACTGGGC CTTTCTCGTG GTGTTCTTGA TCGGATTAAT TGTATCCTGC 
TGTAAAGGGA AGAAGTCAGT CATAGAAGGA GTGGATGAAG ATTCGGACTT AAGTGATGAT 
GAGCCGTCTG CCTACTCTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001297412.1
CDS	268..1671
Translation	MKNQNGTFKDWADANVTVQIENTVLYGYYTLYSVILFCVFFWIPFVYFYYEEKDEDDASKCTQIKTALKYTLGFVVICALLLLVGAFVPLHLPNNNNSTEWEKVKLLFEDLGTGQGLAALSFSISSLTLIGMLAAITYTAYGMSALPLNLIKGTRSTAYERLENTEDIEEVEQHIQTIRSKSKDGRPLPARDRRALKQCEERLRTLRKRERHLEFIENSWWTKFCGALRPLKIIWGIFFILVALLFVISLFLSNLDKALHSAGIDSGFIIFGTNLSNPLNMLLPLLQTVFPLDYILITIIIMYFIFTSMAGIRNIGIWFFWIRLYKIRRGRTRPQALLFLCMILLLIVLHTSYMIYSLAPQYVMYGSQNYLIESNITSDAHKGNSTLAVPKRCDADAPKDQCTVTRTYIFLHKFWFFSAAYYFGNWAFLVVFLIGLIVSCCKGKKSVIEGVDEDSDLSDDEPSAYSA

Target ORF information:

RefSeq Version	NM_001310483.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001310483.1

ORF Insert Sequence:

ATGAAAAATC AAAATGGCAC ATTCAAGGAC TGGGCTGACG CCAATGTCAC CGTACAGATT 
GAGAATACCG TTCTGTATGG CTACTATACT CTGTATTCTG TCATTCTCTT CTGTGTGTTC 
TTCTGGATCC CGTTTGTGTA CTTCTACTAC GAAGAGAAGG ATGAGGACGA CGCCAGCAAA 
TGCACCCAAA TTAAAACGGC GCTCAAGTAC ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG 
CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG CATCTGCCCA ACAACAACAA CTCAACAGAG 
TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC CTTGGAACTG GTCAAGGCCT GGCAGCTCTG 
TCCTTTTCCA TCAGCTCTCT GACCTTGATT GGGATGCTGG CGGCCATAAC CTACACAGCG 
TACGGCATGT CGGCATTACC TTTAAACCTA ATCAAAGGCA CTAGAAGCAC TGCCTACGAG 
CGCCTGGAGA ACACGGAGGA CATCGAGGAA GTGGAGCAGC ACATTCAGAC GATTCGATCC 
AAGAGCAAAG ATGGTCGACC TTTGCCAGCG AGGGATAGAC GTGCCTTAAA GCAATGTGAA 
GAAAGACTAA GAACGCTTAG GAAAAGAGAG CGGCACTTGG AATTCATTGA AAACAGCTGG 
TGGACGAAAT TTTGTGGTGC CCTGCGCCCC CTGAAGATCA TTTGGGGAAT ATTTTTCATC 
CTCGTTGCGT TGCTGTTTGT AATTTCTCTC TTCCTGTCAA ATTTGGATAA AGCTCTTCAT 
TCAGCAGGAA TAGATTCGGG TTTCATCATC TTCGGAACTA ACCTGAGCAA TCCACTGAAC 
ATGCTTTTGC CGTTGTTACA AACAGTGTTC CCTCTTGATT ACATTCTTAT AACAATTATT 
ATTATGTACT TTATTTTTAC TTCGATGGCA GGAATTCGAA ACATTGGCAT TTGGTTCTTT 
TGGATTAGGT TATATAAAAT CAGAAGAGGG CGAACCAGGC CTCAGGCACT CTTATTTCTC 
TGCATGATCC TCCTGCTCAT TGTCCTTCAT ACCAGCTACA TGATCTACAG TCTCGCGCCT 
CAGTACGTGA TGTACGGAAG CCAGAATTAC TTAATAGAGA GCAATATAAC TTCTGATGCC 
CATAAAGGCA ATTCAACCCT CGCTGTGCCA AAAAGATGTG ACGCAGATGC CCCTAAAGAT 
CAGTGTACTG TTACCCGAAC ATACATTTTC CTCCACAAGT TCTGGTTCTT CAGTGCTGCA 
TACTATTTTG GTAACTGGGC CTTTCTCGTG GTGTTCTTGA TCGGATTAAT TGTATCCTGC 
TGTAAAGGGA AGAAGTCAGT CATAGAAGGA GTGGATGAAG ATTCGGACTT AAGTGATGAT 
GAGCCGTCTG CCTACTCTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmbrd1 ( XM_030242602.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030098462.1 Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu85253
Clone ID Related Accession (Same CDS sequence)	XM_030242602.1
Accession Version	XM_030242602.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable lysosomal cobalamin transporter isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAATC AAAATGGCAC ATTCAAGGAC TGGGCTGACG CCAATGTCAC CGTACAGATT 
GAGAATACCG TTCTGTATGG CTACTATACT CTGTATTCTG TCATTCTCTT CTGTGTGTTC 
TTCTGGATCC CGTTTGTGTA CTTCTACTAC GAAGAGAAGG ATGAGGACGA CGCCAGCAAA 
TGCACCCAAA TTAAAACGGC GCTCAAGTAC ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG 
CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG CATCTGCCCA ACAACAACAA CTCAACAGAG 
TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC CTTGGAACTG GTCGCCTGGC AGCTCTGTCC 
TTTTCCATCA GCTCTCTGAC CTTGATTGGG ATGCTGGCGG CCATAACCTA CACAGCGTAC 
GGCATGTCGG CATTACCTTT AAACCTAATC AAAGGCACTA GAAGCACTGC CTACGAGCGC 
CTGGAGAACA CGGAGGACAT CGAGGAAGTG GAGCAGCACA TTCAGACGAT TCGATCCAAG 
AGCAAAGATG GTCGACCTTT GCCAGCGAGG GATAGACGTG CCTTAAAGCA ATGTGAAGAA 
AGACTAAGAA CGCTTAGGAA AAGAGAGCGG CACTTGGAAT TCATTGAAAA CAGCTGGTGG 
ACGAAATTTT GTGGTGCCCT GCGCCCCCTG AAGATCATTT GGGGAATATT TTTCATCCTC 
GTTGCGTTGC TGTTTGTAAT TTCTCTCTTC CTGTCAAATT TGGATAAAGC TCTTCATTCA 
GCAGGAATAG ATTCGGGTTT CATCATCTTC GGAACTAACC TGAGCAATCC ACTGAACATG 
CTTTTGCCGT TGTTACAAAC AGTGTTCCCT CTTGATTACA TTCTTATAAC AATTATTATT 
ATGTACTTTA TTTTTACTTC GATGGCAGGA ATTCGAAACA TTGGCATTTG GTTCTTTTGG 
ATTAGGTTAT ATAAAATCAG AAGAGGGCGA ACCAGGCCTC AGGCACTCTT ATTTCTCTGC 
ATGATCCTCC TGCTCATTGT CCTTCATACC AGCTACATGA TCTACAGTCT CGCGCCTCAG 
TACGTGATGT ACGGAAGCCA GAATTACTTA ATAGAGAGCA ATATAACTTC TGATGCCCAT 
AAAGGCAATT CAACCCTCGC TGTGCCAAAA AGATGTGACG CAGATGCCCC TAAAGATCAG 
TGTACTGTTA CCCGAACATA CATTTTCCTC CACAAGTTCT GGTTCTTCAG TGCTGCATAC 
TATTTTGGTA ACTGGGCCTT TCTCGTGGTG TTCTTGATCG GATTAATTGT ATCCTGCTGT 
AAAGGGAAGA AGTCAGTCAT AGAAGGAGTG GATGAAGATT CGGACTTAAG TGATGATGAG 
CCGTCTGCCT ACTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030098462.1
CDS	292..1692
Translation	MKNQNGTFKDWADANVTVQIENTVLYGYYTLYSVILFCVFFWIPFVYFYYEEKDEDDASKCTQIKTALKYTLGFVVICALLLLVGAFVPLHLPNNNNSTEWEKVKLLFEDLGTGRLAALSFSISSLTLIGMLAAITYTAYGMSALPLNLIKGTRSTAYERLENTEDIEEVEQHIQTIRSKSKDGRPLPARDRRALKQCEERLRTLRKRERHLEFIENSWWTKFCGALRPLKIIWGIFFILVALLFVISLFLSNLDKALHSAGIDSGFIIFGTNLSNPLNMLLPLLQTVFPLDYILITIIIMYFIFTSMAGIRNIGIWFFWIRLYKIRRGRTRPQALLFLCMILLLIVLHTSYMIYSLAPQYVMYGSQNYLIESNITSDAHKGNSTLAVPKRCDADAPKDQCTVTRTYIFLHKFWFFSAAYYFGNWAFLVVFLIGLIVSCCKGKKSVIEGVDEDSDLSDDEPSAYSA

Target ORF information:

RefSeq Version	XM_030242602.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030242602.1

ORF Insert Sequence:

ATGAAAAATC AAAATGGCAC ATTCAAGGAC TGGGCTGACG CCAATGTCAC CGTACAGATT 
GAGAATACCG TTCTGTATGG CTACTATACT CTGTATTCTG TCATTCTCTT CTGTGTGTTC 
TTCTGGATCC CGTTTGTGTA CTTCTACTAC GAAGAGAAGG ATGAGGACGA CGCCAGCAAA 
TGCACCCAAA TTAAAACGGC GCTCAAGTAC ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG 
CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG CATCTGCCCA ACAACAACAA CTCAACAGAG 
TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC CTTGGAACTG GTCGCCTGGC AGCTCTGTCC 
TTTTCCATCA GCTCTCTGAC CTTGATTGGG ATGCTGGCGG CCATAACCTA CACAGCGTAC 
GGCATGTCGG CATTACCTTT AAACCTAATC AAAGGCACTA GAAGCACTGC CTACGAGCGC 
CTGGAGAACA CGGAGGACAT CGAGGAAGTG GAGCAGCACA TTCAGACGAT TCGATCCAAG 
AGCAAAGATG GTCGACCTTT GCCAGCGAGG GATAGACGTG CCTTAAAGCA ATGTGAAGAA 
AGACTAAGAA CGCTTAGGAA AAGAGAGCGG CACTTGGAAT TCATTGAAAA CAGCTGGTGG 
ACGAAATTTT GTGGTGCCCT GCGCCCCCTG AAGATCATTT GGGGAATATT TTTCATCCTC 
GTTGCGTTGC TGTTTGTAAT TTCTCTCTTC CTGTCAAATT TGGATAAAGC TCTTCATTCA 
GCAGGAATAG ATTCGGGTTT CATCATCTTC GGAACTAACC TGAGCAATCC ACTGAACATG 
CTTTTGCCGT TGTTACAAAC AGTGTTCCCT CTTGATTACA TTCTTATAAC AATTATTATT 
ATGTACTTTA TTTTTACTTC GATGGCAGGA ATTCGAAACA TTGGCATTTG GTTCTTTTGG 
ATTAGGTTAT ATAAAATCAG AAGAGGGCGA ACCAGGCCTC AGGCACTCTT ATTTCTCTGC 
ATGATCCTCC TGCTCATTGT CCTTCATACC AGCTACATGA TCTACAGTCT CGCGCCTCAG 
TACGTGATGT ACGGAAGCCA GAATTACTTA ATAGAGAGCA ATATAACTTC TGATGCCCAT 
AAAGGCAATT CAACCCTCGC TGTGCCAAAA AGATGTGACG CAGATGCCCC TAAAGATCAG 
TGTACTGTTA CCCGAACATA CATTTTCCTC CACAAGTTCT GGTTCTTCAG TGCTGCATAC 
TATTTTGGTA ACTGGGCCTT TCTCGTGGTG TTCTTGATCG GATTAATTGT ATCCTGCTGT 
AAAGGGAAGA AGTCAGTCAT AGAAGGAGTG GATGAAGATT CGGACTTAAG TGATGATGAG 
CCGTCTGCCT ACTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmbrd1 ( NM_026719.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080995.2 Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02883
Clone ID Related Accession (Same CDS sequence)	NM_026719.2
Accession Version	NM_026719.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	probable lysosomal cobalamin transporter isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY322390.1, BY291845.1, AK167080.1, AK032560.1, AK158982.1 and AI504365.1. On Jan 20, 2007 this sequence version replaced NM_026719.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660821.163000.1, SRR1660811.80724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCGG CGGCGGCCGA GCTGGTGATC GGCTGGTGCA TCTTCGGCCT CTTGCTCCTG 
GCTATTTTGG CCTTTTGCTG GGTCTACGTT CGGAAGTACC AGAGTCAGCG GGAAAGTGAG 
GTCGTCTCCA CTGTGACAGC CATTTTTTCA CTGGCTGTTG CTCTGATCAC ATCAGCACTG 
CTGCCGGTGG ATATATTTTT GGTTTCTTAC ATGAAAAATC AAAATGGCAC ATTCAAGGAC 
TGGGCTGACG CCAATGTCAC CGTACAGATT GAGAATACCG TTCTGTATGG CTACTATACT 
CTGTATTCTG TCATTCTCTT CTGTGTGTTC TTCTGGATCC CGTTTGTGTA CTTCTACTAC 
GAAGAGAAGG ATGAGGACGA CGCCAGCAAA TGCACCCAAA TTAAAACGGC GCTCAAGTAC 
ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG 
CATCTGCCCA ACAACAACAA CTCAACAGAG TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC 
CTTGGAACTG GTCAAGGCCT GGCAGCTCTG TCCTTTTCCA TCAGCTCTCT GACCTTGATT 
GGGATGCTGG CGGCCATAAC CTACACAGCG TACGGCATGT CGGCATTACC TTTAAACCTA 
ATCAAAGGCA CTAGAAGCAC TGCCTACGAG CGCCTGGAGA ACACGGAGGA CATCGAGGAA 
GTGGAGCAGC ACATTCAGAC GATTCGATCC AAGAGCAAAG ATGGTCGACC TTTGCCAGCG 
AGGGATAGAC GTGCCTTAAA GCAATGTGAA GAAAGACTAA GAACGCTTAG GAAAAGAGAG 
CGGCACTTGG AATTCATTGA AAACAGCTGG TGGACGAAAT TTTGTGGTGC CCTGCGCCCC 
CTGAAGATCA TTTGGGGAAT ATTTTTCATC CTCGTTGCGT TGCTGTTTGT AATTTCTCTC 
TTCCTGTCAA ATTTGGATAA AGCTCTTCAT TCAGCAGGAA TAGATTCGGG TTTCATCATC 
TTCGGAACTA ACCTGAGCAA TCCACTGAAC ATGCTTTTGC CGTTGTTACA AACAGTGTTC 
CCTCTTGATT ACATTCTTAT AACAATTATT ATTATGTACT TTATTTTTAC TTCGATGGCA 
GGAATTCGAA ACATTGGCAT TTGGTTCTTT TGGATTAGGT TATATAAAAT CAGAAGAGGG 
CGAACCAGGC CTCAGGCACT CTTATTTCTC TGCATGATCC TCCTGCTCAT TGTCCTTCAT 
ACCAGCTACA TGATCTACAG TCTCGCGCCT CAGTACGTGA TGTACGGAAG CCAGAATTAC 
TTAATAGAGA GCAATATAAC TTCTGATGCC CATAAAGGCA ATTCAACCCT CGCTGTGCCA 
AAAAGATGTG ACGCAGATGC CCCTAAAGAT CAGTGTACTG TTACCCGAAC ATACATTTTC 
CTCCACAAGT TCTGGTTCTT CAGTGCTGCA TACTATTTTG GTAACTGGGC CTTTCTCGTG 
GTGTTCTTGA TCGGATTAAT TGTATCCTGC TGTAAAGGGA AGAAGTCAGT CATAGAAGGA 
GTGGATGAAG ATTCGGACTT AAGTGATGAT GAGCCGTCTG CCTACTCTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080995.2
CDS	214..1827
Translation	MAAAAAELVIGWCIFGLLLLAILAFCWVYVRKYQSQRESEVVSTVTAIFSLAVALITSALLPVDIFLVSYMKNQNGTFKDWADANVTVQIENTVLYGYYTLYSVILFCVFFWIPFVYFYYEEKDEDDASKCTQIKTALKYTLGFVVICALLLLVGAFVPLHLPNNNNSTEWEKVKLLFEDLGTGQGLAALSFSISSLTLIGMLAAITYTAYGMSALPLNLIKGTRSTAYERLENTEDIEEVEQHIQTIRSKSKDGRPLPARDRRALKQCEERLRTLRKRERHLEFIENSWWTKFCGALRPLKIIWGIFFILVALLFVISLFLSNLDKALHSAGIDSGFIIFGTNLSNPLNMLLPLLQTVFPLDYILITIIIMYFIFTSMAGIRNIGIWFFWIRLYKIRRGRTRPQALLFLCMILLLIVLHTSYMIYSLAPQYVMYGSQNYLIESNITSDAHKGNSTLAVPKRCDADAPKDQCTVTRTYIFLHKFWFFSAAYYFGNWAFLVVFLIGLIVSCCKGKKSVIEGVDEDSDLSDDEPSAYSA

Target ORF information:

RefSeq Version	NM_026719.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026719.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCCGA GCTGGTGATC GGCTGGTGCA TCTTCGGCCT CTTGCTCCTG 
GCTATTTTGG CCTTTTGCTG GGTCTACGTT CGGAAGTACC AGAGTCAGCG GGAAAGTGAG 
GTCGTCTCCA CTGTGACAGC CATTTTTTCA CTGGCTGTTG CTCTGATCAC ATCAGCACTG 
CTGCCGGTGG ATATATTTTT GGTTTCTTAC ATGAAAAATC AAAATGGCAC ATTCAAGGAC 
TGGGCTGACG CCAATGTCAC CGTACAGATT GAGAATACCG TTCTGTATGG CTACTATACT 
CTGTATTCTG TCATTCTCTT CTGTGTGTTC TTCTGGATCC CGTTTGTGTA CTTCTACTAC 
GAAGAGAAGG ATGAGGACGA CGCCAGCAAA TGCACCCAAA TTAAAACGGC GCTCAAGTAC 
ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG 
CATCTGCCCA ACAACAACAA CTCAACAGAG TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC 
CTTGGAACTG GTCAAGGCCT GGCAGCTCTG TCCTTTTCCA TCAGCTCTCT GACCTTGATT 
GGGATGCTGG CGGCCATAAC CTACACAGCG TACGGCATGT CGGCATTACC TTTAAACCTA 
ATCAAAGGCA CTAGAAGCAC TGCCTACGAG CGCCTGGAGA ACACGGAGGA CATCGAGGAA 
GTGGAGCAGC ACATTCAGAC GATTCGATCC AAGAGCAAAG ATGGTCGACC TTTGCCAGCG 
AGGGATAGAC GTGCCTTAAA GCAATGTGAA GAAAGACTAA GAACGCTTAG GAAAAGAGAG 
CGGCACTTGG AATTCATTGA AAACAGCTGG TGGACGAAAT TTTGTGGTGC CCTGCGCCCC 
CTGAAGATCA TTTGGGGAAT ATTTTTCATC CTCGTTGCGT TGCTGTTTGT AATTTCTCTC 
TTCCTGTCAA ATTTGGATAA AGCTCTTCAT TCAGCAGGAA TAGATTCGGG TTTCATCATC 
TTCGGAACTA ACCTGAGCAA TCCACTGAAC ATGCTTTTGC CGTTGTTACA AACAGTGTTC 
CCTCTTGATT ACATTCTTAT AACAATTATT ATTATGTACT TTATTTTTAC TTCGATGGCA 
GGAATTCGAA ACATTGGCAT TTGGTTCTTT TGGATTAGGT TATATAAAAT CAGAAGAGGG 
CGAACCAGGC CTCAGGCACT CTTATTTCTC TGCATGATCC TCCTGCTCAT TGTCCTTCAT 
ACCAGCTACA TGATCTACAG TCTCGCGCCT CAGTACGTGA TGTACGGAAG CCAGAATTAC 
TTAATAGAGA GCAATATAAC TTCTGATGCC CATAAAGGCA ATTCAACCCT CGCTGTGCCA 
AAAAGATGTG ACGCAGATGC CCCTAAAGAT CAGTGTACTG TTACCCGAAC ATACATTTTC 
CTCCACAAGT TCTGGTTCTT CAGTGCTGCA TACTATTTTG GTAACTGGGC CTTTCTCGTG 
GTGTTCTTGA TCGGATTAAT TGTATCCTGC TGTAAAGGGA AGAAGTCAGT CATAGAAGGA 
GTGGATGAAG ATTCGGACTT AAGTGATGAT GAGCCGTCTG CCTACTCTGC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmbrd1 ( XM_017322292.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177781.1 Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77272
Clone ID Related Accession (Same CDS sequence)	XM_017322292.1 , XM_017322292.2
Accession Version	XM_017322292.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable lysosomal cobalamin transporter isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCCGA GCTGGTGATC GGCTGGTGCA TCTTCGGCCT CTTGCTCCTG 
GCTATTTTGG CCTTTTGCTG GGTCTACGTT CGGAAGTACC AGAGTCAGCG GGAAAGTGAG 
GTCGTCTCCA CTGTGACAGC CATTTTTTCA CTGGCTGTTG CTCTGATCAC ATCAGCACTG 
CTGCCGGTGG ATATATTTTT GGTTTCTTAC ATGAAAAATC AAAATGGCAC ATTCAAGGAC 
TGGGCTGACG CCAATGTCAC CGTACAGATT GAGAATACCG TTCTGTATGG CTACTATACT 
CTGTATTCTG TCATTCTCTT CTGTGTGTTC TTCTGGATCC CGTTTGTGTA CTTCTACTAC 
GAAGAGAAGG ATGAGGACGA CGCCAGCAAA TGCACCCAAA TTAAAACGGC GCTCAAGTAC 
ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG 
CATCTGCCCA ACAACAACAA CTCAACAGAG TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC 
CTTGGAACTG GTCGCCTGGC AGCTCTGTCC TTTTCCATCA GCTCTCTGAC CTTGATTGGG 
ATGCTGGCGG CCATAACCTA CACAGCGTAC GGCATGTCGG CATTACCTTT AAACCTAATC 
AAAGGCACTA GAAGCACTGC CTACGAGCGC CTGGAGAACA CGGAGGACAT CGAGGAAGTG 
GAGCAGCACA TTCAGACGAT TCGATCCAAG AGCAAAGATG GTCGACCTTT GCCAGCGAGG 
GATAGACGTG CCTTAAAGCA ATGTGAAGAA AGACTAAGAA CGCTTAGGAA AAGAGAGCGG 
CACTTGGAAT TCATTGAAAA CAGCTGGTGG ACGAAATTTT GTGGTGCCCT GCGCCCCCTG 
AAGATCATTT GGGGAATATT TTTCATCCTC GTTGCGTTGC TGTTTGTAAT TTCTCTCTTC 
CTGTCAAATT TGGATAAAGC TCTTCATTCA GCAGGAATAG ATTCGGGTTT CATCATCTTC 
GGAACTAACC TGAGCAATCC ACTGAACATG CTTTTGCCGT TGTTACAAAC AGTGTTCCCT 
CTTGATTACA TTCTTATAAC AATTATTATT ATGTACTTTA TTTTTACTTC GATGGCAGGA 
ATTCGAAACA TTGGCATTTG GTTCTTTTGG ATTAGGTTAT ATAAAATCAG AAGAGGGCGA 
ACCAGGCCTC AGGCACTCTT ATTTCTCTGC ATGATCCTCC TGCTCATTGT CCTTCATACC 
AGCTACATGA TCTACAGTCT CGCGCCTCAG TACGTGATGT ACGGAAGCCA GAATTACTTA 
ATAGAGAGCA ATATAACTTC TGATGCCCAT AAAGGCAATT CAACCCTCGC TGTGCCAAAA 
AGATGTGACG CAGATGCCCC TAAAGATCAG TGTACTGTTA CCCGAACATA CATTTTCCTC 
CACAAGTTCT GGTTCTTCAG TGCTGCATAC TATTTTGGTA ACTGGGCCTT TCTCGTGGTG 
TTCTTGATCG GATTAATTGT ATCCTGCTGT AAAGGGAAGA AGTCAGTCAT AGAAGGAGTG 
GATGAAGATT CGGACTTAAG TGATGATGAG CCGTCTGCCT ACTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177781.1
CDS	716..2326
Translation	MAAAAAELVIGWCIFGLLLLAILAFCWVYVRKYQSQRESEVVSTVTAIFSLAVALITSALLPVDIFLVSYMKNQNGTFKDWADANVTVQIENTVLYGYYTLYSVILFCVFFWIPFVYFYYEEKDEDDASKCTQIKTALKYTLGFVVICALLLLVGAFVPLHLPNNNNSTEWEKVKLLFEDLGTGRLAALSFSISSLTLIGMLAAITYTAYGMSALPLNLIKGTRSTAYERLENTEDIEEVEQHIQTIRSKSKDGRPLPARDRRALKQCEERLRTLRKRERHLEFIENSWWTKFCGALRPLKIIWGIFFILVALLFVISLFLSNLDKALHSAGIDSGFIIFGTNLSNPLNMLLPLLQTVFPLDYILITIIIMYFIFTSMAGIRNIGIWFFWIRLYKIRRGRTRPQALLFLCMILLLIVLHTSYMIYSLAPQYVMYGSQNYLIESNITSDAHKGNSTLAVPKRCDADAPKDQCTVTRTYIFLHKFWFFSAAYYFGNWAFLVVFLIGLIVSCCKGKKSVIEGVDEDSDLSDDEPSAYSA

Target ORF information:

RefSeq Version	XM_017322292.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322292.1

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCCGA GCTGGTGATC GGCTGGTGCA TCTTCGGCCT CTTGCTCCTG 
GCTATTTTGG CCTTTTGCTG GGTCTACGTT CGGAAGTACC AGAGTCAGCG GGAAAGTGAG 
GTCGTCTCCA CTGTGACAGC CATTTTTTCA CTGGCTGTTG CTCTGATCAC ATCAGCACTG 
CTGCCGGTGG ATATATTTTT GGTTTCTTAC ATGAAAAATC AAAATGGCAC ATTCAAGGAC 
TGGGCTGACG CCAATGTCAC CGTACAGATT GAGAATACCG TTCTGTATGG CTACTATACT 
CTGTATTCTG TCATTCTCTT CTGTGTGTTC TTCTGGATCC CGTTTGTGTA CTTCTACTAC 
GAAGAGAAGG ATGAGGACGA CGCCAGCAAA TGCACCCAAA TTAAAACGGC GCTCAAGTAC 
ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG 
CATCTGCCCA ACAACAACAA CTCAACAGAG TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC 
CTTGGAACTG GTCGCCTGGC AGCTCTGTCC TTTTCCATCA GCTCTCTGAC CTTGATTGGG 
ATGCTGGCGG CCATAACCTA CACAGCGTAC GGCATGTCGG CATTACCTTT AAACCTAATC 
AAAGGCACTA GAAGCACTGC CTACGAGCGC CTGGAGAACA CGGAGGACAT CGAGGAAGTG 
GAGCAGCACA TTCAGACGAT TCGATCCAAG AGCAAAGATG GTCGACCTTT GCCAGCGAGG 
GATAGACGTG CCTTAAAGCA ATGTGAAGAA AGACTAAGAA CGCTTAGGAA AAGAGAGCGG 
CACTTGGAAT TCATTGAAAA CAGCTGGTGG ACGAAATTTT GTGGTGCCCT GCGCCCCCTG 
AAGATCATTT GGGGAATATT TTTCATCCTC GTTGCGTTGC TGTTTGTAAT TTCTCTCTTC 
CTGTCAAATT TGGATAAAGC TCTTCATTCA GCAGGAATAG ATTCGGGTTT CATCATCTTC 
GGAACTAACC TGAGCAATCC ACTGAACATG CTTTTGCCGT TGTTACAAAC AGTGTTCCCT 
CTTGATTACA TTCTTATAAC AATTATTATT ATGTACTTTA TTTTTACTTC GATGGCAGGA 
ATTCGAAACA TTGGCATTTG GTTCTTTTGG ATTAGGTTAT ATAAAATCAG AAGAGGGCGA 
ACCAGGCCTC AGGCACTCTT ATTTCTCTGC ATGATCCTCC TGCTCATTGT CCTTCATACC 
AGCTACATGA TCTACAGTCT CGCGCCTCAG TACGTGATGT ACGGAAGCCA GAATTACTTA 
ATAGAGAGCA ATATAACTTC TGATGCCCAT AAAGGCAATT CAACCCTCGC TGTGCCAAAA 
AGATGTGACG CAGATGCCCC TAAAGATCAG TGTACTGTTA CCCGAACATA CATTTTCCTC 
CACAAGTTCT GGTTCTTCAG TGCTGCATAC TATTTTGGTA ACTGGGCCTT TCTCGTGGTG 
TTCTTGATCG GATTAATTGT ATCCTGCTGT AAAGGGAAGA AGTCAGTCAT AGAAGGAGTG 
GATGAAGATT CGGACTTAAG TGATGATGAG CCGTCTGCCT ACTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lmbrd1 ( XM_017322292.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177781.1 Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu77272
Clone ID Related Accession (Same CDS sequence)	XM_017322292.1 , XM_017322292.2
Accession Version	XM_017322292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable lysosomal cobalamin transporter isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017322292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGCGGCCGA GCTGGTGATC GGCTGGTGCA TCTTCGGCCT CTTGCTCCTG 
GCTATTTTGG CCTTTTGCTG GGTCTACGTT CGGAAGTACC AGAGTCAGCG GGAAAGTGAG 
GTCGTCTCCA CTGTGACAGC CATTTTTTCA CTGGCTGTTG CTCTGATCAC ATCAGCACTG 
CTGCCGGTGG ATATATTTTT GGTTTCTTAC ATGAAAAATC AAAATGGCAC ATTCAAGGAC 
TGGGCTGACG CCAATGTCAC CGTACAGATT GAGAATACCG TTCTGTATGG CTACTATACT 
CTGTATTCTG TCATTCTCTT CTGTGTGTTC TTCTGGATCC CGTTTGTGTA CTTCTACTAC 
GAAGAGAAGG ATGAGGACGA CGCCAGCAAA TGCACCCAAA TTAAAACGGC GCTCAAGTAC 
ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG 
CATCTGCCCA ACAACAACAA CTCAACAGAG TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC 
CTTGGAACTG GTCGCCTGGC AGCTCTGTCC TTTTCCATCA GCTCTCTGAC CTTGATTGGG 
ATGCTGGCGG CCATAACCTA CACAGCGTAC GGCATGTCGG CATTACCTTT AAACCTAATC 
AAAGGCACTA GAAGCACTGC CTACGAGCGC CTGGAGAACA CGGAGGACAT CGAGGAAGTG 
GAGCAGCACA TTCAGACGAT TCGATCCAAG AGCAAAGATG GTCGACCTTT GCCAGCGAGG 
GATAGACGTG CCTTAAAGCA ATGTGAAGAA AGACTAAGAA CGCTTAGGAA AAGAGAGCGG 
CACTTGGAAT TCATTGAAAA CAGCTGGTGG ACGAAATTTT GTGGTGCCCT GCGCCCCCTG 
AAGATCATTT GGGGAATATT TTTCATCCTC GTTGCGTTGC TGTTTGTAAT TTCTCTCTTC 
CTGTCAAATT TGGATAAAGC TCTTCATTCA GCAGGAATAG ATTCGGGTTT CATCATCTTC 
GGAACTAACC TGAGCAATCC ACTGAACATG CTTTTGCCGT TGTTACAAAC AGTGTTCCCT 
CTTGATTACA TTCTTATAAC AATTATTATT ATGTACTTTA TTTTTACTTC GATGGCAGGA 
ATTCGAAACA TTGGCATTTG GTTCTTTTGG ATTAGGTTAT ATAAAATCAG AAGAGGGCGA 
ACCAGGCCTC AGGCACTCTT ATTTCTCTGC ATGATCCTCC TGCTCATTGT CCTTCATACC 
AGCTACATGA TCTACAGTCT CGCGCCTCAG TACGTGATGT ACGGAAGCCA GAATTACTTA 
ATAGAGAGCA ATATAACTTC TGATGCCCAT AAAGGCAATT CAACCCTCGC TGTGCCAAAA 
AGATGTGACG CAGATGCCCC TAAAGATCAG TGTACTGTTA CCCGAACATA CATTTTCCTC 
CACAAGTTCT GGTTCTTCAG TGCTGCATAC TATTTTGGTA ACTGGGCCTT TCTCGTGGTG 
TTCTTGATCG GATTAATTGT ATCCTGCTGT AAAGGGAAGA AGTCAGTCAT AGAAGGAGTG 
GATGAAGATT CGGACTTAAG TGATGATGAG CCGTCTGCCT ACTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177781.1
CDS	1766..3376
Translation	MAAAAAELVIGWCIFGLLLLAILAFCWVYVRKYQSQRESEVVSTVTAIFSLAVALITSALLPVDIFLVSYMKNQNGTFKDWADANVTVQIENTVLYGYYTLYSVILFCVFFWIPFVYFYYEEKDEDDASKCTQIKTALKYTLGFVVICALLLLVGAFVPLHLPNNNNSTEWEKVKLLFEDLGTGRLAALSFSISSLTLIGMLAAITYTAYGMSALPLNLIKGTRSTAYERLENTEDIEEVEQHIQTIRSKSKDGRPLPARDRRALKQCEERLRTLRKRERHLEFIENSWWTKFCGALRPLKIIWGIFFILVALLFVISLFLSNLDKALHSAGIDSGFIIFGTNLSNPLNMLLPLLQTVFPLDYILITIIIMYFIFTSMAGIRNIGIWFFWIRLYKIRRGRTRPQALLFLCMILLLIVLHTSYMIYSLAPQYVMYGSQNYLIESNITSDAHKGNSTLAVPKRCDADAPKDQCTVTRTYIFLHKFWFFSAAYYFGNWAFLVVFLIGLIVSCCKGKKSVIEGVDEDSDLSDDEPSAYSA

Target ORF information:

RefSeq Version	XM_017322292.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus LMBR1 domain containing 1 (Lmbrd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017322292.2

ORF Insert Sequence:

ATGGCGGCGG CGGCGGCCGA GCTGGTGATC GGCTGGTGCA TCTTCGGCCT CTTGCTCCTG 
GCTATTTTGG CCTTTTGCTG GGTCTACGTT CGGAAGTACC AGAGTCAGCG GGAAAGTGAG 
GTCGTCTCCA CTGTGACAGC CATTTTTTCA CTGGCTGTTG CTCTGATCAC ATCAGCACTG 
CTGCCGGTGG ATATATTTTT GGTTTCTTAC ATGAAAAATC AAAATGGCAC ATTCAAGGAC 
TGGGCTGACG CCAATGTCAC CGTACAGATT GAGAATACCG TTCTGTATGG CTACTATACT 
CTGTATTCTG TCATTCTCTT CTGTGTGTTC TTCTGGATCC CGTTTGTGTA CTTCTACTAC 
GAAGAGAAGG ATGAGGACGA CGCCAGCAAA TGCACCCAAA TTAAAACGGC GCTCAAGTAC 
ACTTTGGGAT TTGTTGTGAT CTGCGCTCTG CTGCTTCTCG TTGGTGCCTT TGTGCCTCTG 
CATCTGCCCA ACAACAACAA CTCAACAGAG TGGGAAAAGG TGAAGCTCCT GTTTGAAGAC 
CTTGGAACTG GTCGCCTGGC AGCTCTGTCC TTTTCCATCA GCTCTCTGAC CTTGATTGGG 
ATGCTGGCGG CCATAACCTA CACAGCGTAC GGCATGTCGG CATTACCTTT AAACCTAATC 
AAAGGCACTA GAAGCACTGC CTACGAGCGC CTGGAGAACA CGGAGGACAT CGAGGAAGTG 
GAGCAGCACA TTCAGACGAT TCGATCCAAG AGCAAAGATG GTCGACCTTT GCCAGCGAGG 
GATAGACGTG CCTTAAAGCA ATGTGAAGAA AGACTAAGAA CGCTTAGGAA AAGAGAGCGG 
CACTTGGAAT TCATTGAAAA CAGCTGGTGG ACGAAATTTT GTGGTGCCCT GCGCCCCCTG 
AAGATCATTT GGGGAATATT TTTCATCCTC GTTGCGTTGC TGTTTGTAAT TTCTCTCTTC 
CTGTCAAATT TGGATAAAGC TCTTCATTCA GCAGGAATAG ATTCGGGTTT CATCATCTTC 
GGAACTAACC TGAGCAATCC ACTGAACATG CTTTTGCCGT TGTTACAAAC AGTGTTCCCT 
CTTGATTACA TTCTTATAAC AATTATTATT ATGTACTTTA TTTTTACTTC GATGGCAGGA 
ATTCGAAACA TTGGCATTTG GTTCTTTTGG ATTAGGTTAT ATAAAATCAG AAGAGGGCGA 
ACCAGGCCTC AGGCACTCTT ATTTCTCTGC ATGATCCTCC TGCTCATTGT CCTTCATACC 
AGCTACATGA TCTACAGTCT CGCGCCTCAG TACGTGATGT ACGGAAGCCA GAATTACTTA 
ATAGAGAGCA ATATAACTTC TGATGCCCAT AAAGGCAATT CAACCCTCGC TGTGCCAAAA 
AGATGTGACG CAGATGCCCC TAAAGATCAG TGTACTGTTA CCCGAACATA CATTTTCCTC 
CACAAGTTCT GGTTCTTCAG TGCTGCATAC TATTTTGGTA ACTGGGCCTT TCTCGTGGTG 
TTCTTGATCG GATTAATTGT ATCCTGCTGT AAAGGGAAGA AGTCAGTCAT AGAAGGAGTG 
GATGAAGATT CGGACTTAAG TGATGATGAG CCGTCTGCCT ACTCTGCGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Single allele Lmbrd1 knockout results in cardiac hypertrophy.
Journal of the Formosan Medical Association = Taiwan yi zhi(2017 May)
Tseng LT,Lin CL,Pan KH,Tzen KY,Su MJ,Tsai CT,Li YH,Li PC,Chiang FT,Chang SC,Chang MF

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Lmbrd1 expression is essential for the initiation of gastrulation.
Journal of cellular and molecular medicine20(8)1523-33(2016 08)
Buers I,Pennekamp P,Nitschke Y,Lowe C,Skryabin BV,Rutsch F

Regulation of Hematopoiesis and Methionine Homeostasis by mTORC1 Inhibitor NPRL2.
Cell reports12(3)371-9(2015 Jul)
Dutchak PA,Laxman S,Estill SJ,Wang C,Wang Y,Wang Y,Bulut GB,Gao J,Huang LJ,Tu BP

LMBD1 protein serves as a specific adaptor for insulin receptor internalization.
The Journal of biological chemistry288(45)32424-32(2013 Nov)
Tseng LT,Lin CL,Tzen KY,Chang SC,Chang MF

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Lmbrd1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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