Pdhb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdhb
Entrez Gene ID	68263
Full Name	pyruvate dehydrogenase (lipoamide) beta
Synonyms	2610103L06Rik,AL024199,C81408
General protein information	Preferred Names pyruvate dehydrogenase (lipoamide) beta Names pyruvate dehydrogenase E1 component subunit beta, mitochondrial PDHE1-B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 A1

mRNA and Protein(s)

mRNA	Protein	Name
XM_030247984.1	XP_030103844.1	pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform X1
NM_024221.3	NP_077183.1	pyruvate dehydrogenase E1 component subunit beta, mitochondrial precursor

Pathways from BioSystems

Homologies

Schizosaccharomyces pombe (fission yeast)	pdb1	NP_596272.1
Bos taurus (cattle)	PDHB	NP_001030512.2
Danio rerio (zebrafish)	pdhb	NP_998319.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010421	XP_311527.2
Neurospora crassa	NCU03004	XP_965390.1
Xenopus tropicalis (tropical clawed frog)	pdhb	NP_001016212.1
Mus musculus (house mouse)	Pdhb	NP_077183.1
Rattus norvegicus (Norway rat)	Pdhb	NP_001007621.1
Gallus gallus (chicken)	PDHB	NP_001185549.1
Caenorhabditis elegans (roundworm)	pdhb-1	NP_500340.1
Saccharomyces cerevisiae (baker's yeast)	PDB1	NP_009780.1
Kluyveromyces lactis	KLLA0F09603g	XP_455516.1
Arabidopsis thaliana (thale cress)	MAB1	NP_199898.1
Oryza sativa (rice)	Os08g0536000	NP_001062360.1
Homo sapiens (human)	PDHB	NP_000916.2
Pan troglodytes (chimpanzee)	PDHB	NP_001233347.1
	PDHB	XP_001096445.1
Canis lupus familiaris (dog)	PDHB	XP_533778.2
Magnaporthe oryzae (rice blast fungus)	MGG_10569	XP_003721383.1
Drosophila melanogaster (fruit fly)	CG11876	NP_651668.1
Eremothecium gossypii	AGOS_AAR167C	NP_982710.1
Oryza sativa (rice)	Os09g0509200	NP_001063627.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

Prolyl-hydroxylase PHD3 interacts with pyruvate dehydrogenase (PDH)-E1? and regulates the cellular PDH activity.
Kikuchi D, Minamishima YA, Nakayama K
Biochemical and biophysical research communications451(2)288-94(2014 Aug)

Long-range enhancers regulating Myc expression are required for normal facial morphogenesis.
Uslu VV, Petretich M, Ruf S, Langenfeld K, Fonseca NA, Marioni JC, Spitz F
Nature genetics46(7)753-8(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PHD3 depletion did not affect the expression of the PDH-E1alpha, E1beta, and E2 subunits, or the phosphorylation status of E1alpha, but destabilized the PDH complex (PDC), resulting in less functional PDC.
Title: Prolyl-hydroxylase PHD3 interacts with pyruvate dehydrogenase (PDH)-E1? and regulates the cellular PDH activity.

The following Pdhb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdhb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14751	NM_024221.3 Latest version!	Mus musculus pyruvate dehydrogenase (lipoamide) beta (Pdhb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu14751	XM_030247984.1 Latest version!	Mus musculus pyruvate dehydrogenase (lipoamide) beta (Pdhb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdhb ( NM_024221.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_077183.1 Mus musculus pyruvate dehydrogenase (lipoamide) beta (Pdhb), mRNA.

Price & Availability↑

CloneID	OMu14751
Clone ID Related Accession (Same CDS sequence)	XM_030247984.1 , NM_024221.3
Accession Version	NM_024221.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	pyruvate dehydrogenase E1 component subunit beta, mitochondrial precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ159324.1, AK153058.1 and BY414585.1. On Jul 7, 2006 this sequence version replaced NM_024221.2. ##Evidence-Data-START## Transcript exon combination :: AK166631.1, SRR1660815.321377.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTGG TGGCTGGTCT GGTGCGGGGA CCCTTGCGGC AGGCTTCCGG GCTGCTGAAG 
AGGCGTTTTC ACCGCTCGGC GCCCGCAGCG GTGCAGTTGA CAGTTCGTGA AGCTATTAAT 
CAAGGTATGG ATGAAGAACT AGAAAGAGAT GAAAAAGTTT TTCTGCTTGG GGAAGAAGTT 
GCCCAGTATG ACGGTGCATA CAAGGTTAGC AGAGGCCTGT GGAAGAAATA CGGTGACAAG 
AGGATCATCG ACACCCCCAT CTCAGAGATG GGCTTTGCTG GAATTGCTGT TGGTGCAGCT 
ATGGCTGGGT TGCGGCCCAT CTGTGAATTT ATGACCTTCA ATTTCTCTAT GCAAGCTATT 
GACCAGGTTA TAAACTCAGC TGCCAAGACT TACTATATGT CCGCTGGCCT TCAGCCTGTG 
CCCATAGTAT TCAGGGGGCC CAATGGCGCC TCAGCAGGTG TAGCTGCTCA GCACTCGCAA 
TGCTTTGCTG CATGGTATGG GCACTGCCCA GGTTTAAAAG TGGTCAGCCC CTGGAATTCC 
GAGGATGCAA AAGGACTTAT TAAATCGGCC ATTCGTGATA ATAACCCAGT GGTAATGCTA 
GAAAATGAAC TGATGTATGG AGTTGCATTT GAACTTCCTG CAGAAGCTCA GTCAAAAGAT 
TTCCTGATTC CTATTGGAAA AGCCAAAATT GAAAGGCAAG GGACCCACAT CACTGTAGTT 
GCCCATTCAA GACCAGTGGG CCACTGCCTA GAAGCTGCAG CTGTATTGTC TAAGGAGGGA 
ATTGAATGTG AGGTAATAAA TCTGCGCACC ATCAGACCAA TGGACATCGA AGCCATAGAA 
GCCAGTGTGA TGAAGACAAA TCATCTCGTG ACTGTGGAAG GAGGCTGGCC ACAATTTGGA 
GTAGGAGCTG AGATTTGTGC CAGAATTATG GAAGGCCCTG CATTCAACTT CCTTGATGCC 
CCTGCCGTGC GTGTCACTGG TGCTGATGTC CCTATGCCTT ACGCAAAGGT CCTAGAAGAC 
AACTCCGTAC CTCAGGTCAA AGACATCATA TTTGCAGTAA AGAAGACTCT GAACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077183.1
CDS	61..1140
Translation	MAVVAGLVRGPLRQASGLLKRRFHRSAPAAVQLTVREAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSAGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVMLENELMYGVAFELPAEAQSKDFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKVLEDNSVPQVKDIIFAVKKTLNI

Target ORF information:

RefSeq Version	NM_024221.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate dehydrogenase (lipoamide) beta (Pdhb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024221.3

ORF Insert Sequence:

ATGGCGGTGG TGGCTGGTCT GGTGCGGGGA CCCTTGCGGC AGGCTTCCGG GCTGCTGAAG 
AGGCGTTTTC ACCGCTCGGC GCCCGCAGCG GTGCAGTTGA CAGTTCGTGA AGCTATTAAT 
CAAGGTATGG ATGAAGAACT AGAAAGAGAT GAAAAAGTTT TTCTGCTTGG GGAAGAAGTT 
GCCCAGTATG ACGGTGCATA CAAGGTTAGC AGAGGCCTGT GGAAGAAATA CGGTGACAAG 
AGGATCATCG ACACCCCCAT CTCAGAGATG GGCTTTGCTG GAATTGCTGT TGGTGCAGCT 
ATGGCTGGGT TGCGGCCCAT CTGTGAATTT ATGACCTTCA ATTTCTCTAT GCAAGCTATT 
GACCAGGTTA TAAACTCAGC TGCCAAGACT TACTATATGT CCGCTGGCCT TCAGCCTGTG 
CCCATAGTAT TCAGGGGGCC CAATGGCGCC TCAGCAGGTG TAGCTGCTCA GCACTCGCAA 
TGCTTTGCTG CATGGTATGG GCACTGCCCA GGTTTAAAAG TGGTCAGCCC CTGGAATTCC 
GAGGATGCAA AAGGACTTAT TAAATCGGCC ATTCGTGATA ATAACCCAGT GGTAATGCTA 
GAAAATGAAC TGATGTATGG AGTTGCATTT GAACTTCCTG CAGAAGCTCA GTCAAAAGAT 
TTCCTGATTC CTATTGGAAA AGCCAAAATT GAAAGGCAAG GGACCCACAT CACTGTAGTT 
GCCCATTCAA GACCAGTGGG CCACTGCCTA GAAGCTGCAG CTGTATTGTC TAAGGAGGGA 
ATTGAATGTG AGGTAATAAA TCTGCGCACC ATCAGACCAA TGGACATCGA AGCCATAGAA 
GCCAGTGTGA TGAAGACAAA TCATCTCGTG ACTGTGGAAG GAGGCTGGCC ACAATTTGGA 
GTAGGAGCTG AGATTTGTGC CAGAATTATG GAAGGCCCTG CATTCAACTT CCTTGATGCC 
CCTGCCGTGC GTGTCACTGG TGCTGATGTC CCTATGCCTT ACGCAAAGGT CCTAGAAGAC 
AACTCCGTAC CTCAGGTCAA AGACATCATA TTTGCAGTAA AGAAGACTCT GAACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pdhb ( XM_030247984.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103844.1 Mus musculus pyruvate dehydrogenase (lipoamide) beta (Pdhb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu14751
Clone ID Related Accession (Same CDS sequence)	XM_030247984.1 , NM_024221.3
Accession Version	XM_030247984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	pyruvate dehydrogenase E1 component subunit beta, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGTGG TGGCTGGTCT GGTGCGGGGA CCCTTGCGGC AGGCTTCCGG GCTGCTGAAG 
AGGCGTTTTC ACCGCTCGGC GCCCGCAGCG GTGCAGTTGA CAGTTCGTGA AGCTATTAAT 
CAAGGTATGG ATGAAGAACT AGAAAGAGAT GAAAAAGTTT TTCTGCTTGG GGAAGAAGTT 
GCCCAGTATG ACGGTGCATA CAAGGTTAGC AGAGGCCTGT GGAAGAAATA CGGTGACAAG 
AGGATCATCG ACACCCCCAT CTCAGAGATG GGCTTTGCTG GAATTGCTGT TGGTGCAGCT 
ATGGCTGGGT TGCGGCCCAT CTGTGAATTT ATGACCTTCA ATTTCTCTAT GCAAGCTATT 
GACCAGGTTA TAAACTCAGC TGCCAAGACT TACTATATGT CCGCTGGCCT TCAGCCTGTG 
CCCATAGTAT TCAGGGGGCC CAATGGCGCC TCAGCAGGTG TAGCTGCTCA GCACTCGCAA 
TGCTTTGCTG CATGGTATGG GCACTGCCCA GGTTTAAAAG TGGTCAGCCC CTGGAATTCC 
GAGGATGCAA AAGGACTTAT TAAATCGGCC ATTCGTGATA ATAACCCAGT GGTAATGCTA 
GAAAATGAAC TGATGTATGG AGTTGCATTT GAACTTCCTG CAGAAGCTCA GTCAAAAGAT 
TTCCTGATTC CTATTGGAAA AGCCAAAATT GAAAGGCAAG GGACCCACAT CACTGTAGTT 
GCCCATTCAA GACCAGTGGG CCACTGCCTA GAAGCTGCAG CTGTATTGTC TAAGGAGGGA 
ATTGAATGTG AGGTAATAAA TCTGCGCACC ATCAGACCAA TGGACATCGA AGCCATAGAA 
GCCAGTGTGA TGAAGACAAA TCATCTCGTG ACTGTGGAAG GAGGCTGGCC ACAATTTGGA 
GTAGGAGCTG AGATTTGTGC CAGAATTATG GAAGGCCCTG CATTCAACTT CCTTGATGCC 
CCTGCCGTGC GTGTCACTGG TGCTGATGTC CCTATGCCTT ACGCAAAGGT CCTAGAAGAC 
AACTCCGTAC CTCAGGTCAA AGACATCATA TTTGCAGTAA AGAAGACTCT GAACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103844.1
CDS	198..1277
Translation	MAVVAGLVRGPLRQASGLLKRRFHRSAPAAVQLTVREAINQGMDEELERDEKVFLLGEEVAQYDGAYKVSRGLWKKYGDKRIIDTPISEMGFAGIAVGAAMAGLRPICEFMTFNFSMQAIDQVINSAAKTYYMSAGLQPVPIVFRGPNGASAGVAAQHSQCFAAWYGHCPGLKVVSPWNSEDAKGLIKSAIRDNNPVVMLENELMYGVAFELPAEAQSKDFLIPIGKAKIERQGTHITVVAHSRPVGHCLEAAAVLSKEGIECEVINLRTIRPMDIEAIEASVMKTNHLVTVEGGWPQFGVGAEICARIMEGPAFNFLDAPAVRVTGADVPMPYAKVLEDNSVPQVKDIIFAVKKTLNI

Target ORF information:

RefSeq Version	XM_030247984.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus pyruvate dehydrogenase (lipoamide) beta (Pdhb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247984.1

ORF Insert Sequence:

ATGGCGGTGG TGGCTGGTCT GGTGCGGGGA CCCTTGCGGC AGGCTTCCGG GCTGCTGAAG 
AGGCGTTTTC ACCGCTCGGC GCCCGCAGCG GTGCAGTTGA CAGTTCGTGA AGCTATTAAT 
CAAGGTATGG ATGAAGAACT AGAAAGAGAT GAAAAAGTTT TTCTGCTTGG GGAAGAAGTT 
GCCCAGTATG ACGGTGCATA CAAGGTTAGC AGAGGCCTGT GGAAGAAATA CGGTGACAAG 
AGGATCATCG ACACCCCCAT CTCAGAGATG GGCTTTGCTG GAATTGCTGT TGGTGCAGCT 
ATGGCTGGGT TGCGGCCCAT CTGTGAATTT ATGACCTTCA ATTTCTCTAT GCAAGCTATT 
GACCAGGTTA TAAACTCAGC TGCCAAGACT TACTATATGT CCGCTGGCCT TCAGCCTGTG 
CCCATAGTAT TCAGGGGGCC CAATGGCGCC TCAGCAGGTG TAGCTGCTCA GCACTCGCAA 
TGCTTTGCTG CATGGTATGG GCACTGCCCA GGTTTAAAAG TGGTCAGCCC CTGGAATTCC 
GAGGATGCAA AAGGACTTAT TAAATCGGCC ATTCGTGATA ATAACCCAGT GGTAATGCTA 
GAAAATGAAC TGATGTATGG AGTTGCATTT GAACTTCCTG CAGAAGCTCA GTCAAAAGAT 
TTCCTGATTC CTATTGGAAA AGCCAAAATT GAAAGGCAAG GGACCCACAT CACTGTAGTT 
GCCCATTCAA GACCAGTGGG CCACTGCCTA GAAGCTGCAG CTGTATTGTC TAAGGAGGGA 
ATTGAATGTG AGGTAATAAA TCTGCGCACC ATCAGACCAA TGGACATCGA AGCCATAGAA 
GCCAGTGTGA TGAAGACAAA TCATCTCGTG ACTGTGGAAG GAGGCTGGCC ACAATTTGGA 
GTAGGAGCTG AGATTTGTGC CAGAATTATG GAAGGCCCTG CATTCAACTT CCTTGATGCC 
CCTGCCGTGC GTGTCACTGG TGCTGATGTC CCTATGCCTT ACGCAAAGGT CCTAGAAGAC 
AACTCCGTAC CTCAGGTCAA AGACATCATA TTTGCAGTAA AGAAGACTCT GAACATCTGA