Chac2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chac2
Entrez Gene ID	68044
Full Name	ChaC, cation transport regulator 2
General protein information	Preferred Names ChaC, cation transport regulator 2 Names putative glutathione-specific gamma-glutamylcyclotransferase 2 ChaC, cation transport regulator homolog 2 cation transport regulator-like protein 2 gamma-GCG 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 A4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001290667.1	NP_001277596.1	putative glutathione-specific gamma-glutamylcyclotransferase 2 isoform 2
NM_026527.3	NP_080803.1	putative glutathione-specific gamma-glutamylcyclotransferase 2 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CHAC2	NP_001068996.1
Homo sapiens (human)	CHAC2	NP_001008708.1
	CHAC2	XP_002799271.1
Canis lupus familiaris (dog)	CHAC2	XP_854835.2
Schizosaccharomyces pombe (fission yeast)	SPBC31F10.03	NP_596565.1
Danio rerio (zebrafish)	chac2	NP_001025128.1
Saccharomyces cerevisiae (baker's yeast)	GCG1	NP_011090.3
Magnaporthe oryzae (rice blast fungus)	MGG_04817	XP_003713747.1
Oryza sativa (rice)	Os04g0448600	NP_001052924.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002216	XP_307968.3
Neurospora crassa	NCU02737	XP_965122.1
Xenopus tropicalis (tropical clawed frog)	chac2	NP_001017137.1
Pan troglodytes (chimpanzee)	CHAC2	XP_515472.2
Mus musculus (house mouse)	Chac2	NP_080803.1
Rattus norvegicus (Norway rat)	Chac2	NP_001020187.1
Gallus gallus (chicken)	CHAC2	NP_001026203.1
Drosophila melanogaster (fruit fly)	CG2540	NP_572818.1
Kluyveromyces lactis	KLLA0C11649g	XP_452720.1
Eremothecium gossypii	AGOS_AFR184C	NP_985731.1
Arabidopsis thaliana (thale cress)	AT1G44790	NP_564490.1

Gene Ontology
Bibliography

Related articles in PubMed

ChaC2, an Enzyme for Slow Turnover of Cytosolic Glutathione.
Kaur A, Gautam R, Srivastava R, Chandel A, Kumar A, Karthikeyan S, Bachhawat AK
The Journal of biological chemistry292(2)638-651(2017 Jan)

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Piao Y, Ko NT, Lim MK, Ko MS
Genome research11(9)1553-8(2001 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Human and mouse ChaC2 proteins purified in vitro show 10-20-fold lower catalytic efficiency than ChaC1, although they showed comparable Km values
Title: ChaC2, an Enzyme for Slow Turnover of Cytosolic Glutathione.

The following Chac2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chac2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu13951	NM_026527.3 Latest version!	Mus musculus ChaC, cation transport regulator 2 (Chac2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu16875	NM_001290667.1 Latest version!	Mus musculus ChaC, cation transport regulator 2 (Chac2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chac2 ( NM_026527.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080803.1 Mus musculus ChaC, cation transport regulator 2 (Chac2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu13951
Clone ID Related Accession (Same CDS sequence)	NM_026527.3
Accession Version	NM_026527.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	putative glutathione-specific gamma-glutamylcyclotransferase 2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY333527.1, AK078993.1 and AL662891.9. On Mar 15, 2014 this sequence version replaced NM_026527.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC025100.1, AK010914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGTGT TTGGTTATGG GTCCCTCATC TGGAAGGTGG ACTTCCCCTA CCAGGACAAG 
CTGGTTGGTT ACATCACAAA CTACAGTCGG CGCTTCTGGC AAGGTAGCAC GGACCACCGC 
GGGGTCCCAG GCAAGCCTGG AAGAGTTGTG ACCCTTGTTG AAGATCCTGG GGGCAGTGTA 
TGGGGTGTTG CTTACAAACT ACCAGTAGGA AAAGAAGAGG AAGTAAAAAC ATACCTTGAC 
TTCAGAGAGA AAGGAGGCTA CAGAACTACG ACAGTCATTT TTTATCCAAA AGATTCCACA 
ACAAAACCAT TCAGTGTTTT GCTCTACATT GGAACATGTG ATAATCCTAA CTATCTTGGC 
CCTGCACCTC TGGAAGATAT TGCTGAACAA ATTTTTAATG CAGCTGGTCC AAGTGGAAGA 
AATACAGAAT ATCTTTTTGA ACTTGCAGAT TCTGTTAGGA AACTTGTACC AGAAGATGCA 
GACGAGCATC TCTTCTCTTT GGAAAAATTA GTAAAGGAAC GCTTAGAAGG AAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080803.1
CDS	120..656
Translation	MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKPGRVVTLVEDPGGSVWGVAYKLPVGKEEEVKTYLDFREKGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPEDADEHLFSLEKLVKERLEGK

Target ORF information:

RefSeq Version	NM_026527.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ChaC, cation transport regulator 2 (Chac2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_026527.3

ORF Insert Sequence:

ATGTGGGTGT TTGGTTATGG GTCCCTCATC TGGAAGGTGG ACTTCCCCTA CCAGGACAAG 
CTGGTTGGTT ACATCACAAA CTACAGTCGG CGCTTCTGGC AAGGTAGCAC GGACCACCGC 
GGGGTCCCAG GCAAGCCTGG AAGAGTTGTG ACCCTTGTTG AAGATCCTGG GGGCAGTGTA 
TGGGGTGTTG CTTACAAACT ACCAGTAGGA AAAGAAGAGG AAGTAAAAAC ATACCTTGAC 
TTCAGAGAGA AAGGAGGCTA CAGAACTACG ACAGTCATTT TTTATCCAAA AGATTCCACA 
ACAAAACCAT TCAGTGTTTT GCTCTACATT GGAACATGTG ATAATCCTAA CTATCTTGGC 
CCTGCACCTC TGGAAGATAT TGCTGAACAA ATTTTTAATG CAGCTGGTCC AAGTGGAAGA 
AATACAGAAT ATCTTTTTGA ACTTGCAGAT TCTGTTAGGA AACTTGTACC AGAAGATGCA 
GACGAGCATC TCTTCTCTTT GGAAAAATTA GTAAAGGAAC GCTTAGAAGG AAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chac2 ( NM_001290667.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001277596.1 Mus musculus ChaC, cation transport regulator 2 (Chac2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16875
Clone ID Related Accession (Same CDS sequence)	NM_001290667.1
Accession Version	NM_001290667.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	putative glutathione-specific gamma-glutamylcyclotransferase 2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY333527.1, AK021391.1, AK010914.1 and AL662891.9. On Mar 15, 2014 this sequence version replaced XM_006514799.1. Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK021391.1, BQ445465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGTGT TTGGTTATGG GTCCCTCATC TGGAAGGTGG ACTTCCCCTA CCAGGACAAG 
CTGGTTGGTT ACATCACAAA CTACAGTCGG CGCTTCTGGC AAGGTAGCAC GGACCACCGC 
GGGGTCCCAG GCAAGGGCAG TGTATGGGGT GTTGCTTACA AACTACCAGT AGGAAAAGAA 
GAGGAAGTAA AAACATACCT TGACTTCAGA GAGAAAGGAG GCTACAGAAC TACGACAGTC 
ATTTTTTATC CAAAAGATTC CACAACAAAA CCATTCAGTG TTTTGCTCTA CATTGGAACA 
TGTGATAATC CTAACTATCT TGGCCCTGCA CCTCTGGAAG ATATTGCTGA ACAAATTTTT 
AATGCAGCTG GTCCAAGTGG AAGAAATACA GAATATCTTT TTGAACTTGC AGATTCTGTT 
AGGAAACTTG TACCAGAAGA TGCAGACGAG CATCTCTTCT CTTTGGAAAA ATTAGTAAAG 
GAACGCTTAG AAGGAAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277596.1
CDS	120..620
Translation	MWVFGYGSLIWKVDFPYQDKLVGYITNYSRRFWQGSTDHRGVPGKGSVWGVAYKLPVGKEEEVKTYLDFREKGGYRTTTVIFYPKDSTTKPFSVLLYIGTCDNPNYLGPAPLEDIAEQIFNAAGPSGRNTEYLFELADSVRKLVPEDADEHLFSLEKLVKERLEGK

Target ORF information:

RefSeq Version	NM_001290667.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ChaC, cation transport regulator 2 (Chac2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290667.1

ORF Insert Sequence:

ATGTGGGTGT TTGGTTATGG GTCCCTCATC TGGAAGGTGG ACTTCCCCTA CCAGGACAAG 
CTGGTTGGTT ACATCACAAA CTACAGTCGG CGCTTCTGGC AAGGTAGCAC GGACCACCGC 
GGGGTCCCAG GCAAGGGCAG TGTATGGGGT GTTGCTTACA AACTACCAGT AGGAAAAGAA 
GAGGAAGTAA AAACATACCT TGACTTCAGA GAGAAAGGAG GCTACAGAAC TACGACAGTC 
ATTTTTTATC CAAAAGATTC CACAACAAAA CCATTCAGTG TTTTGCTCTA CATTGGAACA 
TGTGATAATC CTAACTATCT TGGCCCTGCA CCTCTGGAAG ATATTGCTGA ACAAATTTTT 
AATGCAGCTG GTCCAAGTGG AAGAAATACA GAATATCTTT TTGAACTTGC AGATTCTGTT 
AGGAAACTTG TACCAGAAGA TGCAGACGAG CATCTCTTCT CTTTGGAAAA ATTAGTAAAG 
GAACGCTTAG AAGGAAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ChaC2, an Enzyme for Slow Turnover of Cytosolic Glutathione.
The Journal of biological chemistry292(2)638-651(2017 Jan)
Kaur A,Gautam R,Srivastava R,Chandel A,Kumar A,Karthikeyan S,Bachhawat AK

Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.
Genome research11(9)1553-8(2001 Sep)
Piao Y,Ko NT,Lim MK,Ko MS