Wfdc1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wfdc1
Entrez Gene ID	67866
Full Name	WAP four-disulfide core domain 1
Synonyms	2310058A03Rik,ps20
General protein information	Preferred Names WAP four-disulfide core domain 1 Names WAP four-disulfide core domain protein 1 prostate stromal protein ps20 ps20 growth inhibitor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_023395.2	NP_075884.1	WAP four-disulfide core domain protein 1 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Wfdc1	NP_075884.1
Canis lupus familiaris (dog)	WFDC1	XP_005620699.1
Bos taurus (cattle)	WFDC1	XP_005218473.1
Pan troglodytes (chimpanzee)	WFDC1	XP_511141.2
Homo sapiens (human)	WFDC1	NP_067020.2
Rattus norvegicus (Norway rat)	Wfdc1	NP_598265.1

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

WFDC1 is a key modulator of inflammatory and wound repair responses.
Ressler SJ, Dang TD, Wu SM, Tse DY, Gilbert BE, Vyakarnam A, Yang F, Schauer IG, Barron DA, Rowley DR
The American journal of pathology184(11)2951-64(2014 Nov)

Notch2 regulates BMP signaling and epithelial morphogenesis in the ciliary body of the mouse eye.
Zhou Y, Tanzie C, Yan Z, Chen S, Duncan M, Gaudenz K, Li H, Seidel C, Lewis B, Moran A, Libby RT, Kiernan AE, Xie T
Proceedings of the National Academy of Sciences of the United States of America110(22)8966-71(2013 May)

WFDC1/ps20: a host factor that influences the neutrophil response to murine hepatitis virus (MHV) 1 infection.
Rogers E, Wang BX, Cui Z, Rowley DR, Ressler SJ, Vyakarnam A, Fish EN
Antiviral research96(2)158-68(2012 Nov)

Increased ?-catenin activity in the anterior neural plate induces ectopic mid-hindbrain characteristics.
Paek H, Antoine MW, Diaz F, H?bert JM
Developmental dynamics : an official publication of the American Association of Anatomists241(2)242-6(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WFDC1/ps20 modulates core host response mechanisms, in part, via regulation of osteopontin and MMP-9 activity. Release from WFDC1 regulation is likely a key component of inflammatory and repair response mechanisms.
Title: WFDC1 is a key modulator of inflammatory and wound repair responses.

These data suggest roles for ps20 in regulating expression of neutrophil-specific chemotactic factors, thereby potentially modulating neutrophil migration, and in modulating neutrophil susceptibility to murine hepatitis virus -1 infection.
Title: WFDC1/ps20: a host factor that influences the neutrophil response to murine hepatitis virus (MHV) 1 infection.

ps20 may be a TGF-beta1-induced regulator of angiogenesis that functions by either promoting endothelial cell migration or by contributing to pericyte stabilization of newly formed vascular structures.
Title: Promotion of angiogenesis by ps20 in the differential reactive stroma prostate cancer xenograft model.

The following Wfdc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wfdc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu02242	NM_023395.2 Latest version!	Mus musculus WAP four-disulfide core domain 1 (Wfdc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wfdc1 ( NM_023395.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_075884.1 Mus musculus WAP four-disulfide core domain 1 (Wfdc1), mRNA.

Price & Availability↑

CloneID	OMu02242
Clone ID Related Accession (Same CDS sequence)	NM_023395.2
Accession Version	NM_023395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Mus musculus(house mouse)
Product	WAP four-disulfide core domain protein 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK132541.1 and AK161606.1. On Dec 22, 2005 this sequence version replaced NM_023395.1. ##Evidence-Data-START## Transcript exon combination :: AK161606.1, AK132541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAACT GCGGCAGAAA AGTCCTTCGG GCTCTGAGTT TCCTGTTGCT ACTGGGCTCC 
AGTTCTGCCC AGGGCACTTG GGAGGCAATG CTGCCGGCCA GGCTGGCCGA GAAGTCCCGA 
GCTGAAGAGG TTGCAGCGAC AGGCTCCCGG CAGCCCCATG CAGACCGCTG CCCACCACCG 
CCAAGGACAC TGCCCCCGGG TGCCTGTCAG GCCACACGCT GCCAGGCTGA CTCTGAGTGC 
CCACGACACA GGCGCTGCTG CTACAATGGC TGTGCCTATG CCTGCCTGGA GGCGGTGCCA 
CCTCCGCCAG TTCTAGACTG GCTGGTGCAG CCCAAACCAC GATGGCTGGG TGGCAACGGC 
TGGCTGCTGG ACGGTCCTGA GGAAGTGTTA CAAGCAGAGA CTTGCAGCAC CACTGAGGAT 
GGGGCAGAGC CACTCCTCTG TCCCTCAGGC TATGAGTGCC ATATCCTGCA GCCAGGGGAC 
GAAGCCCAGG GCATACCCAA CCATGGACAG TGTGTTAAGC AACGCCGGCA AGCAGAAGGG 
CGGGTCCTGC GACAGAGGCT TCACAAGGAA TATCCAGAAG GTGACTCCAA GAATGTGGCA 
GAGCCTGGGA AGGGACAACA GAGGCACTTT CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075884.1
CDS	177..812
Translation	MGNCGRKVLRALSFLLLLGSSSAQGTWEAMLPARLAEKSRAEEVAATGSRQPHADRCPPPPRTLPPGACQATRCQADSECPRHRRCCYNGCAYACLEAVPPPPVLDWLVQPKPRWLGGNGWLLDGPEEVLQAETCSTTEDGAEPLLCPSGYECHILQPGDEAQGIPNHGQCVKQRRQAEGRVLRQRLHKEYPEGDSKNVAEPGKGQQRHFP

Target ORF information:

RefSeq Version	NM_023395.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus WAP four-disulfide core domain 1 (Wfdc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023395.2

ORF Insert Sequence:

ATGGGTAACT GCGGCAGAAA AGTCCTTCGG GCTCTGAGTT TCCTGTTGCT ACTGGGCTCC 
AGTTCTGCCC AGGGCACTTG GGAGGCAATG CTGCCGGCCA GGCTGGCCGA GAAGTCCCGA 
GCTGAAGAGG TTGCAGCGAC AGGCTCCCGG CAGCCCCATG CAGACCGCTG CCCACCACCG 
CCAAGGACAC TGCCCCCGGG TGCCTGTCAG GCCACACGCT GCCAGGCTGA CTCTGAGTGC 
CCACGACACA GGCGCTGCTG CTACAATGGC TGTGCCTATG CCTGCCTGGA GGCGGTGCCA 
CCTCCGCCAG TTCTAGACTG GCTGGTGCAG CCCAAACCAC GATGGCTGGG TGGCAACGGC 
TGGCTGCTGG ACGGTCCTGA GGAAGTGTTA CAAGCAGAGA CTTGCAGCAC CACTGAGGAT 
GGGGCAGAGC CACTCCTCTG TCCCTCAGGC TATGAGTGCC ATATCCTGCA GCCAGGGGAC 
GAAGCCCAGG GCATACCCAA CCATGGACAG TGTGTTAAGC AACGCCGGCA AGCAGAAGGG 
CGGGTCCTGC GACAGAGGCT TCACAAGGAA TATCCAGAAG GTGACTCCAA GAATGTGGCA 
GAGCCTGGGA AGGGACAACA GAGGCACTTT CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

WFDC1 is a key modulator of inflammatory and wound repair responses.
The American journal of pathology184(11)2951-64(2014 Nov)
Ressler SJ,Dang TD,Wu SM,Tse DY,Gilbert BE,Vyakarnam A,Yang F,Schauer IG,Barron DA,Rowley DR

Notch2 regulates BMP signaling and epithelial morphogenesis in the ciliary body of the mouse eye.
Proceedings of the National Academy of Sciences of the United States of America110(22)8966-71(2013 May)
Zhou Y,Tanzie C,Yan Z,Chen S,Duncan M,Gaudenz K,Li H,Seidel C,Lewis B,Moran A,Libby RT,Kiernan AE,Xie T

WFDC1/ps20: a host factor that influences the neutrophil response to murine hepatitis virus (MHV) 1 infection.
Antiviral research96(2)158-68(2012 Nov)
Rogers E,Wang BX,Cui Z,Rowley DR,Ressler SJ,Vyakarnam A,Fish EN

Increased ?-catenin activity in the anterior neural plate induces ectopic mid-hindbrain characteristics.
Developmental dynamics : an official publication of the American Association of Anatomists241(2)242-6(2012 Feb)
Paek H,Antoine MW,Diaz F,H?bert JM