Ube2g1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2g1
Entrez Gene ID	67128
Full Name	ubiquitin-conjugating enzyme E2G 1
Synonyms	2700059C12Rik,AI256795,AU014992,AW552068,D130023C12Rik
General protein information	Preferred Names ubiquitin-conjugating enzyme E2G 1 Names ubiquitin-conjugating enzyme E2 G1 E2 ubiquitin-conjugating enzyme G1 E217K UBC7 ubiquitin carrier protein G1 ubiquitin-conjugating enzyme E2G 1 (UBC7 homolog, C. elegans) ubiquitin-protein ligase G1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 B4

mRNA and Protein(s)

mRNA	Protein	Name
NM_025985.4	NP_080261.2	ubiquitin-conjugating enzyme E2 G1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2G1	XP_001174528.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002251	XP_307933.4
Bos taurus (cattle)	UBE2G1	NP_001075927.1
Rattus norvegicus (Norway rat)	Ube2g1	NP_073181.1
Homo sapiens (human)	UBE2G1	NP_003333.1
Canis lupus familiaris (dog)	UBE2G1	XP_005620370.1
Arabidopsis thaliana (thale cress)	UBC13	NP_566884.1
Oryza sativa (rice)	Os01g0839700	NP_001044755.1
Xenopus tropicalis (tropical clawed frog)	ube2g1	NP_989051.1
	LOC721528	XP_001117702.2
Gallus gallus (chicken)	UBE2G1	NP_001074352.1
Danio rerio (zebrafish)	ube2g1a	NP_956157.1
Drosophila melanogaster (fruit fly)	CG40045	NP_001036640.1
Schizosaccharomyces pombe (fission yeast)	ubc15	NP_596465.1
Arabidopsis thaliana (thale cress)	UBC7	NP_568902.2
Mus musculus (house mouse)	Ube2g1	NP_080261.2
Oryza sativa (rice)	Os05g0460200	NP_001055755.1

Gene Ontology
Bibliography

Related articles in PubMed

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Steinmetz CC, Tatavarty V, Sugino K, Shima Y, Joseph A, Lin H, Rutlin M, Lambo M, Hempel CM, Okaty BW, Paradis S, Nelson SB, Turrigiano GG
Cell reports16(10)2711-2722(2016 09)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Evsikov AV, Graber JH, Brockman JM, Hampl A, Holbrook AE, Singh P, Eppig JJ, Solter D, Knowles BB
Genes & development20(19)2713-27(2006 Oct)

Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains.
Ye Y, Meyer HH, Rapoport TA
The Journal of cell biology162(1)71-84(2003 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube2g1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2g1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu01363	NM_025985.4 Latest version!	Mus musculus ubiquitin-conjugating enzyme E2G 1 (Ube2g1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2g1 ( NM_025985.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_080261.2 Mus musculus ubiquitin-conjugating enzyme E2G 1 (Ube2g1), mRNA.

Price & Availability↑

CloneID	OMu01363
Clone ID Related Accession (Same CDS sequence)	NM_025985.4
Accession Version	NM_025985.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	ubiquitin-conjugating enzyme E2 G1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV657669.1, AK167472.1 and AL808023.3. On Sep 22, 2006 this sequence version replaced NM_025985.3. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.48353.1, SRR1660817.281194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGAGC TGCAGTCGGC GCTGCTACTG CGAAGACAGC TGGCAGAACT CAACAAAAAT 
CCAGTGGAAG GCTTTTCAGC AGGTTTAATA GACGACAATG ATCTCTATCG TTGGGAAGTC 
CTTATTATTG GTCCTCCAGA TACACTTTAT GAAGGTGGTG TTTTTAAGGC TCATCTCACT 
TTCCCAAAAG ATTATCCCCT CCGGCCTCCT AAAATGAAAT TCATTACAGA GATTTGGCAC 
CCAAATGTTG ATAAAAATGG TGATGTTTGC ATTTCTATTC TTCATGAGCC TGGGGAGGAT 
AAATATGGTT ATGAGAAGCC AGAGGAACGC TGGTTACCTA TCCATACTGT GGAAACCATC 
ATGATTAGTG TCATTTCTAT GCTGGCAGAT CCTAATGGAG ACTCACCTGC AAATGTAGAT 
GCTGCGAAAG AATGGAGGGA AGACAGAAAC GGAGAATTTA AAAGGAAAGT TGCCCGCTGT 
GTAAGAAAAA GCCAAGAAAC TGCTTTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_080261.2
CDS	330..842
Translation	MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKSQETAFE

Target ORF information:

RefSeq Version	NM_025985.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ubiquitin-conjugating enzyme E2G 1 (Ube2g1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025985.4

ORF Insert Sequence:

ATGACGGAGC TGCAGTCGGC GCTGCTACTG CGAAGACAGC TGGCAGAACT CAACAAAAAT 
CCAGTGGAAG GCTTTTCAGC AGGTTTAATA GACGACAATG ATCTCTATCG TTGGGAAGTC 
CTTATTATTG GTCCTCCAGA TACACTTTAT GAAGGTGGTG TTTTTAAGGC TCATCTCACT 
TTCCCAAAAG ATTATCCCCT CCGGCCTCCT AAAATGAAAT TCATTACAGA GATTTGGCAC 
CCAAATGTTG ATAAAAATGG TGATGTTTGC ATTTCTATTC TTCATGAGCC TGGGGAGGAT 
AAATATGGTT ATGAGAAGCC AGAGGAACGC TGGTTACCTA TCCATACTGT GGAAACCATC 
ATGATTAGTG TCATTTCTAT GCTGGCAGAT CCTAATGGAG ACTCACCTGC AAATGTAGAT 
GCTGCGAAAG AATGGAGGGA AGACAGAAAC GGAGAATTTA AAAGGAAAGT TGCCCGCTGT 
GTAAGAAAAA GCCAAGAAAC TGCTTTTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Upregulation of ?3A Drives Homeostatic Plasticity by Rerouting AMPAR into the Recycling Endosomal Pathway.
Cell reports16(10)2711-2722(2016 09)
Steinmetz CC,Tatavarty V,Sugino K,Shima Y,Joseph A,Lin H,Rutlin M,Lambo M,Hempel CM,Okaty BW,Paradis S,Nelson SB,Turrigiano GG

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

Cracking the egg: molecular dynamics and evolutionary aspects of the transition from the fully grown oocyte to embryo.
Genes & development20(19)2713-27(2006 Oct)
Evsikov AV,Graber JH,Brockman JM,Hampl A,Holbrook AE,Singh P,Eppig JJ,Solter D,Knowles BB

Function of the p97-Ufd1-Npl4 complex in retrotranslocation from the ER to the cytosol: dual recognition of nonubiquitinated polypeptide segments and polyubiquitin chains.
The Journal of cell biology162(1)71-84(2003 Jul)
Ye Y,Meyer HH,Rapoport TA