Nek7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nek7
Entrez Gene ID	59125
Full Name	NIMA (never in mitosis gene a)-related expressed kinase 7
Synonyms	2810460C19Rik,AU020186
General protein information	Preferred Names NIMA (never in mitosis gene a)-related expressed kinase 7 Names serine/threonine-protein kinase Nek7 NIMA-related expressed kinase 7 NIMA-related kinase 7 never in mitosis A-related kinase 7 nimA-related protein kinase 7
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E4\|1 60.82 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001311148.1	NP_001298077.1	serine/threonine-protein kinase Nek7 isoform 2
NM_021605.4	NP_067618.1	serine/threonine-protein kinase Nek7 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NEK7	XP_003587160.1
Rattus norvegicus (Norway rat)	Nek7	NP_001101816.1
Caenorhabditis elegans (roundworm)	nekl-3	NP_510080.2
Xenopus tropicalis (tropical clawed frog)	nek7	NP_001072574.1
Canis lupus familiaris (dog)	NEK7	XP_857029.1
Gallus gallus (chicken)	NEK7	NP_001026435.1
Danio rerio (zebrafish)	nek7	NP_001003617.1
Homo sapiens (human)	NEK7	NP_598001.1
Pan troglodytes (chimpanzee)	NEK7	XP_003949711.1
	NEK7	XP_002802037.1
Mus musculus (house mouse)	Nek7	NP_067618.1

Gene Ontology
Bibliography

Related articles in PubMed

NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component.
Shi H, Wang Y, Li X, Zhan X, Tang M, Fina M, Su L, Pratt D, Bu CH, Hildebrand S, Lyon S, Scott L, Quan J, Sun Q, Russell J, Arnett S, Jurek P, Chen D, Kravchenko VV, Mathison JC, Moresco EM, Monson NL, Ulevitch RJ, Beutler B
Nature immunology17(3)250-8(2016 Mar)

NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux.
He Y, Zeng MY, Yang D, Motro B, N??ez G
Nature530(7590)354-7(2016 Feb)

A Genome-wide CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Screen Identifies NEK7 as an Essential Component of NLRP3 Inflammasome Activation.
Schmid-Burgk JL, Chauhan D, Schmidt T, Ebert TS, Reinhardt J, Endl E, Hornung V
The Journal of biological chemistry291(1)103-9(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NEK7 serves as a cellular switch that enforces mutual exclusivity of the inflammasome response and cell division.
Title: NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component.

NEK7 is an essential protein that acts downstream of potassium efflux to mediate NLRP3 inflammasome assembly and activation
Title: NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux.

murine macrophages deficient in Nek7 displayed a largely blunted Nlrp3 inflammasome response.
Title: A Genome-wide CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Screen Identifies NEK7 as an Essential Component of NLRP3 Inflammasome Activation.

results confirm Nek7 as a regulator of cell division, and reveal it as an essential component for mammalian growth and survival
Title: Nek7 kinase targeting leads to early mortality, cytokinesis disturbance and polyploidy.

During early embryogenesis nek7 is expressed in the site of decidual reaction. Later in embryogenesis, nek7 is highly expressed in the dorsal thalamus.
Title: The related murine kinases, Nek6 and Nek7, display distinct patterns of expression.

The following Nek7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nek7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu69832	NM_001311148.1 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu12496	NM_021605.4 Latest version!	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nek7 ( NM_001311148.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001298077.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69832
Clone ID Related Accession (Same CDS sequence)	NM_001311148.1
Accession Version	NM_001311148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek7 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY170715.1, AK149308.1, AK035502.1, BE626935.1 and BQ174652.1. On Jul 24, 2015 this sequence version replaced XM_006529770.2. Transcript Variant: This variant (2) lacks two consecutive coding exons compared to variant 1, that causes a frameshift. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.98996.1, AK165583.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164140 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAAC AATCACAAGG AATGCAAGGG CCGCCCGTTA CTCAGTTCCA GCCACAGAAG 
GCATTACGGC CAGATATGGG CTATAATACT TTAGCCAACT TCCGAATAGA AAAGAAAATT 
GGTCGTGGAC AATTTAGTGA AGTTTATAGA GCATCCTGTC TCTTGGATGG AGTGCCGGTA 
GCGTTAAAAA AAGTACAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGTATC 
AAAGAAATAG ACCTCCTTAA GCAACTCAAC CATCCAAATG TAATTAAATA CTATGCATCA 
TTCATTGAGG ATAATGAGCT GAACATAGTT TTGGAGTTAG CAGATGCTGG TGACCTCTCC 
AGAATGATAA AGCACTTTAA GAAACAAAAG AGGCTAATCC CTGAGAGAAC CGTTTGGAAA 
TACTTCGTTC AGCTCTGCAG TGCACTGGAC CACATGCATT CTCGAAGAGT CATGCACAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TAGTAAAACT CGGAGACCTT 
GGGCTTGGTC GGTTTTTCAG CTCCAAAACC ACAGCTGCAC ATTCTTTAGC TACGACAGCT 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001298077.1
CDS	233..838
Translation	MDEQSQGMQGPPVTQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRASCLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALDHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLATTAS

Target ORF information:

RefSeq Version	NM_001311148.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001311148.1

ORF Insert Sequence:

ATGGATGAAC AATCACAAGG AATGCAAGGG CCGCCCGTTA CTCAGTTCCA GCCACAGAAG 
GCATTACGGC CAGATATGGG CTATAATACT TTAGCCAACT TCCGAATAGA AAAGAAAATT 
GGTCGTGGAC AATTTAGTGA AGTTTATAGA GCATCCTGTC TCTTGGATGG AGTGCCGGTA 
GCGTTAAAAA AAGTACAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGTATC 
AAAGAAATAG ACCTCCTTAA GCAACTCAAC CATCCAAATG TAATTAAATA CTATGCATCA 
TTCATTGAGG ATAATGAGCT GAACATAGTT TTGGAGTTAG CAGATGCTGG TGACCTCTCC 
AGAATGATAA AGCACTTTAA GAAACAAAAG AGGCTAATCC CTGAGAGAAC CGTTTGGAAA 
TACTTCGTTC AGCTCTGCAG TGCACTGGAC CACATGCATT CTCGAAGAGT CATGCACAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TAGTAAAACT CGGAGACCTT 
GGGCTTGGTC GGTTTTTCAG CTCCAAAACC ACAGCTGCAC ATTCTTTAGC TACGACAGCT 
AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nek7 ( NM_021605.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067618.1 Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12496
Clone ID Related Accession (Same CDS sequence)	NM_021605.4
Accession Version	NM_021605.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	serine/threonine-protein kinase Nek7 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY170715.1, AK149308.1, AK035502.1, BE626935.1 and BQ174652.1. On Jul 24, 2015 this sequence version replaced NM_021605.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660815.250429.1, SRR1660817.41705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAAC AATCACAAGG AATGCAAGGG CCGCCCGTTA CTCAGTTCCA GCCACAGAAG 
GCATTACGGC CAGATATGGG CTATAATACT TTAGCCAACT TCCGAATAGA AAAGAAAATT 
GGTCGTGGAC AATTTAGTGA AGTTTATAGA GCATCCTGTC TCTTGGATGG AGTGCCGGTA 
GCGTTAAAAA AAGTACAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGTATC 
AAAGAAATAG ACCTCCTTAA GCAACTCAAC CATCCAAATG TAATTAAATA CTATGCATCA 
TTCATTGAGG ATAATGAGCT GAACATAGTT TTGGAGTTAG CAGATGCTGG TGACCTCTCC 
AGAATGATAA AGCACTTTAA GAAACAAAAG AGGCTAATCC CTGAGAGAAC CGTTTGGAAA 
TACTTCGTTC AGCTCTGCAG TGCACTGGAC CACATGCATT CTCGAAGAGT CATGCACAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TAGTAAAACT CGGAGACCTT 
GGGCTTGGTC GGTTTTTCAG CTCCAAAACC ACAGCTGCAC ATTCTTTAGT GGGTACACCT 
TACTACATGT CTCCAGAGAG AATACATGAA AATGGATACA ACTTCAAGTC TGACATCTGG 
TCTCTTGGCT GTCTGCTATA TGAGATGGCT GCACTGCAGA GTCCTTTCTA CGGCGACAAG 
ATGAACTTGT ATTCTCTGTG TAAGAAGATA GAGCAGTGTG ACTACCCGCC TCTCCCGTCA 
GATCACTATT CGGAGGAGCT ACGACAGCTA GTTAATATAT GCATCAACCC AGATCCAGAG 
AAGCGACCCG ACATCGCCTA TGTTTATGAT GTGGCAAAGA GGATGCATGC ATGTACCGCA 
AGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067618.1
CDS	233..1141
Translation	MDEQSQGMQGPPVTQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYRASCLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYASFIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALDHMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTPYYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKIEQCDYPPLPSDHYSEELRQLVNICINPDPEKRPDIAYVYDVAKRMHACTAST

Target ORF information:

RefSeq Version	NM_021605.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021605.4

ORF Insert Sequence:

ATGGATGAAC AATCACAAGG AATGCAAGGG CCGCCCGTTA CTCAGTTCCA GCCACAGAAG 
GCATTACGGC CAGATATGGG CTATAATACT TTAGCCAACT TCCGAATAGA AAAGAAAATT 
GGTCGTGGAC AATTTAGTGA AGTTTATAGA GCATCCTGTC TCTTGGATGG AGTGCCGGTA 
GCGTTAAAAA AAGTACAGAT ATTTGATTTA ATGGATGCCA AAGCACGTGC TGATTGTATC 
AAAGAAATAG ACCTCCTTAA GCAACTCAAC CATCCAAATG TAATTAAATA CTATGCATCA 
TTCATTGAGG ATAATGAGCT GAACATAGTT TTGGAGTTAG CAGATGCTGG TGACCTCTCC 
AGAATGATAA AGCACTTTAA GAAACAAAAG AGGCTAATCC CTGAGAGAAC CGTTTGGAAA 
TACTTCGTTC AGCTCTGCAG TGCACTGGAC CACATGCATT CTCGAAGAGT CATGCACAGA 
GATATAAAAC CAGCTAATGT GTTCATTACA GCCACTGGGG TAGTAAAACT CGGAGACCTT 
GGGCTTGGTC GGTTTTTCAG CTCCAAAACC ACAGCTGCAC ATTCTTTAGT GGGTACACCT 
TACTACATGT CTCCAGAGAG AATACATGAA AATGGATACA ACTTCAAGTC TGACATCTGG 
TCTCTTGGCT GTCTGCTATA TGAGATGGCT GCACTGCAGA GTCCTTTCTA CGGCGACAAG 
ATGAACTTGT ATTCTCTGTG TAAGAAGATA GAGCAGTGTG ACTACCCGCC TCTCCCGTCA 
GATCACTATT CGGAGGAGCT ACGACAGCTA GTTAATATAT GCATCAACCC AGATCCAGAG 
AAGCGACCCG ACATCGCCTA TGTTTATGAT GTGGCAAAGA GGATGCATGC ATGTACCGCA 
AGCACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component.
Nature immunology17(3)250-8(2016 Mar)
Shi H,Wang Y,Li X,Zhan X,Tang M,Fina M,Su L,Pratt D,Bu CH,Hildebrand S,Lyon S,Scott L,Quan J,Sun Q,Russell J,Arnett S,Jurek P,Chen D,Kravchenko VV,Mathison JC,Moresco EM,Monson NL,Ulevitch RJ,Beutler B

NEK7 is an essential mediator of NLRP3 activation downstream of potassium efflux.
Nature530(7590)354-7(2016 Feb)
He Y,Zeng MY,Yang D,Motro B,N??ez G

A Genome-wide CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) Screen Identifies NEK7 as an Essential Component of NLRP3 Inflammasome Activation.
The Journal of biological chemistry291(1)103-9(2016 Jan)
Schmid-Burgk JL,Chauhan D,Schmidt T,Ebert TS,Reinhardt J,Endl E,Hornung V