Med12 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Med12
Entrez Gene ID	59024
Full Name	mediator complex subunit 12
Synonyms	230kDa,Mopa,OPA-1,Tnrc11,Trap230
General protein information	Preferred Names mediator complex subunit 12 Names mediator of RNA polymerase II transcription subunit 12 OPA-containing protein 1 mediator of RNA polymerase II transcription, subunit 12 homolog thyroid hormone receptor-associated protein complex 230 kDa component trinucleotide repeat containing 11 (THR-associated protein) trinucleotide repeat containing 11 (THR-associated protein, 230 kDa subunit) trinucleotide repeat-containing gene 11 protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X\|X D

mRNA and Protein(s)

mRNA	Protein	Name
NM_021521.2	NP_067496.2	mediator of RNA polymerase II transcription subunit 12

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED12	NP_005111.2
	MED12	XP_001088424.2
Rattus norvegicus (Norway rat)	Med12	NP_001180221.1
Danio rerio (zebrafish)	med12	NP_001034550.1
Pan troglodytes (chimpanzee)	MED12	NP_001009019.1
Bos taurus (cattle)	MED12	NP_001192807.1
Xenopus tropicalis (tropical clawed frog)	med12	XP_002934949.2
Gallus gallus (chicken)	MED12	XP_004940691.1
Mus musculus (house mouse)	Med12	NP_067496.2
Canis lupus familiaris (dog)	MED12	XP_003640267.1

Gene Ontology
Bibliography

Related articles in PubMed

MED12 regulates a transcriptional network of calcium-handling genes in the heart.
Baskin KK, Makarewich CA, DeLeon SM, Ye W, Chen B, Beetz N, Schrewe H, Bassel-Duby R, Olson EN
JCI insight2(14)(2017 Jul)

Med12 regulates ovarian steroidogenesis, uterine development and maternal effects in the mammalian egg.
Wang X, Mittal P, Castro CA, Rajkovic G, Rajkovic A
Biology of reproduction97(6)822-834(2017 Jan)

MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.
Aranda-Orgilles B, Salda?a-Meyer R, Wang E, Trompouki E, Fassl A, Lau S, Mullenders J, Rocha PP, Raviram R, Guillamot M, S?nchez-D?az M, Wang K, Kayembe C, Zhang N, Amoasii L, Choudhuri A, Skok JA, Schober M, Reinberg D, Sicinski P, Schrewe H, Tsirigos A, Zon LI, Aifantis I
Cell stem cell19(6)784-799(2016 Dec)

Dual role of Med12 in PRC1-dependent gene repression and ncRNA-mediated transcriptional activation.
Papadopoulou T, Kaymak A, Sayols S, Richly H
Cell cycle (Georgetown, Tex.)15(11)1479-93(2016 Jun)

Mediator subunit Med12 contributes to the maintenance of neural stem cell identity.
Kim NH, Livi CB, Yew PR, Boyer TG
BMC developmental biology16(1)17(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

alterations in MED12-dependent enhancer regulation may control both physiological and malignant hematopoiesis.
Title: MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.

The data reveal how the interplay between PRC1, ncRNA and Mediator complexes controls pluripotency and cellular differentiation.
Title: Dual role of Med12 in PRC1-dependent gene repression and ncRNA-mediated transcriptional activation.

Study provide evidence that Med12 regulates neural stem cell gene expression programs linked to adhesion and cell cycle progression but suppresses its adhesion by repressing the expression of cell adhesion molecules.
Title: Mediator subunit Med12 contributes to the maintenance of neural stem cell identity.

Med1 and Med12, two subunits of the mediator complex implicated in transcription initiation and long-range enhancer/promoter loop formation, are dynamically recruited to the IgH locus enhancers.
Title: Mediator facilitates transcriptional activation and dynamic long-range contacts at the IgH locus during class switch recombination.

leiomyomatous, Med12 c.131G>A variant-expressing uteri developed chromosomal rearrangements.
Title: Med12 gain-of-function mutation causes leiomyomas and genomic instability.

The following Med12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Med12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14907	NM_021521.2 Latest version!	Mus musculus mediator complex subunit 12 (Med12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1609.30 $2299.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Med12 ( NM_021521.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067496.2 Mus musculus mediator complex subunit 12 (Med12), mRNA.

Price & Availability↑

CloneID	OMu14907
Clone ID Related Accession (Same CDS sequence)	NM_021521.2
Accession Version	NM_021521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6573bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mediator of RNA polymerase II transcription subunit 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC090654.1, BC057119.1 and BC110696.1. On Feb 10, 2007 this sequence version replaced NM_021521.1. Sequence Note: This sequence has been modified as follows: removed 16 bp suspected to be vector contamination from the 5' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC090654.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131, SAMN01164138 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCTT TCGGGATCTT GAGCTATGAA CACCGACCCC TGAAGCGGCT GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCTTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ACGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATCAT CGCAGAGAAG 
TTAAGGTGTA ATACTCTCTC TGACACTGGT CGCAGGAAGT CCCTAATGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC AAGATCTCAG AGTGCTATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AACCACTCAC ACACCTAGCC AAAAAGGTCC CCATTTTCAG TAAAAAGGAA 
GAAGTATTTG GGTATTTGGC CAAATACACG GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACTT GTGCCTACTA TGCAGCAATG TCTGAGACTA AGGTTAAGAA GAAAAATACT 
GCTGACCCCT TCACTGAATG GACTCAAATC ATCACAAAGT ACTTGTGGGA GCAGCTACAG 
AAGATGGCTG AGTACTATCG GCCAGGGCCT GCAGGAAGTG GAGGCTGTGG TTCTACTATA 
GGGCCTTTGC CCCATGATGT AGAGATGGCC ATCAGGCAGT GGGATTACAA TGAGAAGCTC 
GCCTTGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC TTGGGTGCTT 
GAGTGTTTTG AGAAAATACG CCCTGGAGAA GATGAATTGC TTAAATTGCT GCTTCCCCTA 
CTGCTGCGAT ACTCAGGGGA GTTTGTTCAG TCTGCCTATC TGTCCCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGATTGGC TCTCCAGCTG GATGGTGTGA GCAGCCACTC ATCTCATGTC 
ATAGCTGCTC AGTCAACAAG TTCTCTGCCC ACTACCCCAG CACCTCAGCC CCCAACTAGC 
AGTACACCCT CGACTCCCTT TAGTGACCTG CTTATGTGCC CTCAGCATCG TCCCCTGGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTTCTGTGTT GCCCTAGTGC CCTAGTTTGG 
CACTACTCAT TGACTGATAG CCGAATTAAG ACCGGCTCAC CACTTGACCA CCTGCCTATT 
GCTCCTTCCA ACTTGCCCAT GCCAGAGGGT AACAGTGCCT TTACTCAGCA GGTCCGTGCA 
AAATTACGAG AGATCGAACA ACAGATCAAG GAGCGTGGAC AAGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGTTTCACCA TTGGACGGGT GCTCCATACT 
TTAGAAGTGC TGGATAGCCA TAGTTTCGAG CGCTCTGACT TTAGTAACTC TCTTGACTCC 
CTTTGTAATC GAATCTTTGG ATTGGGGCCT AGTAAGGATG GTCATGAGAT TTCCTCTGAT 
GATGATGCTG TGGTATCATT ATTGTGTGAA TGGGCTGTAA GCTGCAAACG CTCAGGTCGG 
CATCGTGCTA TGGTAGTAGC CAAACTCCTA GAGAAGAGAC AAGCAGAAAT TGAGGCTGAG 
CGCTGCGGAG AATCTGAAGC AGCTGATGAG AAGGGCTCCG TCGCCTCTGG TTCCCTTTCC 
GCTCCTAGTG CACCCATTTT CCAGGATGTC CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACTG ATCCCCGAAG TGAAAGTGAG CGAGTAGAAT TCTTTAACTT GGTACTGCTC 
TTCTGTGAAC TGATCCGACA TGATGTCTTC TCCCATAATA TGTACACCTG CACTCTCATC 
TCTCGGGGGG ATCTTGCATT TGGAGCCCCT GGTCCTCGGC CTCCCTCTCC CTTTGATGAT 
CCTACAGATG ATCCAGAGCG CAAGGAGGCT GAAGGCAGCA GCAGCAGCAA GCTAGAGGAC 
CCAGGGCTCT CTGAATCTAT GGACATCGAC CCTAGTTCCA GTGTGCTTTT TGAGGACATG 
GAAAAGCCTG ATTTCTCATT GTTCTCCCCT ACTATGCCTT GTGAGGGGAA GGGAAGCCCA 
TCCCCTGAGA AACCAGATGT CGAAAAGGAA GTAAAACCCC CAGCCAAAGA GAAGATCGAG 
GGGACACTTG GGATTCTGTA TGACCAGCCA CGACATGTGC AGTATGCCAC ACACTTTCCA 
ATCCCACAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGGAAGC AGCGAGATGA TGCCCGCCAT GCCATCAAGA AGATTACCAA GGATATCCTG 
AAGGTTCTGA ATCGCAAGGG GACAGCAGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAAGATGGGC AGAAGCGACG ACGCAACCGG 
CCCGAAGCCT TCCCCACTGC TGAAGATATT TTTGCTAAGT TCCAGCACCT TTCCCATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAACGTTC TGGAGCAGAT CACGAGCTTC 
GCCCTTGGCA TGTCGTACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTGAGCAT CAGTGGCCTC ATCGACTTTG CCATCCAGTT ACTGAATGAG 
CTGAGTGTGG TTGAGGCGGA GCTCCTTCTC AAATCGTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCTTATGTAT CGTGGCTGTC CTTCGACACT ATCATGCCTG CCTCATCCTC 
AATCAGGACC AGATGGCGCA AGTGTTTGAG GGGCTCTGTG GCGTGGTGAA ACATGGAATG 
AACCGTTCAG ATGGCTCCTC TGCAGAGCGC TGTATCCTTG CTTATCTCTA TGATCTGTAT 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGTGACTT TTGCTCAAAA 
GTGAAGAACA CCATCTACTG TAACGTGGAG CCATCGGAAT CCAATATGCG CTGGGCACCG 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCCGCTCACA CTTTCACCTA CACGGGGCTC 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTATAGCT TTGTGTGCAA TGCTCTTATG 
CACGTCTGCG TGGGCCACCA TGATCCTGAT AGGGTAAATG ACATCGCCAT CCTTTGTGCA 
GAGCTGACCG GCTATTGCAA GTCCTTGAGT GCTGAGTGGC TAGGAGTACT TAAGGCCTTG 
TGCTGCTCCT CGAACAATGG CACTTGTGGT TTCAATGATC TCCTGTGCAA CGTAGACGTC 
AGTGATTTGT CTTTTCATGA TTCCTTGGCT ACTTTTGTTG CTATCCTCAT CGCCCGTCAA 
TGTTTGCTCC TAGAAGACCT GATTCGCTGT GCAGCCATCC CTTCACTCCT CAATGCTGCT 
TGTAGTGAGC AGGATTCTGA GCCAGGAGCC AGGCTTACTT GCCGAATCCT CCTCCACCTG 
TTCAAGACGC CACAACTCAA TCCTTGCCAG TCTGATGGAA ACAAACCTAC TGTTGGAATC 
CGGTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGGGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTCGGAGATG CGGAGCTAAA AGGTTCAGGC 
TTTACTGTGC CGGGAGGAAC AGAAGAACTT CCAGAAGAGG AGGGAGGAGG TGGTAGTAGC 
GGTCGGAGAC AGGGTGGCCG CAACATCTCT GTGGAGACAG CAAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGAAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAGTCA 
CTGTGTGAGG ATAGCAATGA TCTACAAGAC CCAGTGTTGA GTAGTGCCCA GGCCCAGCGC 
CTCATGCAAC TTATCTGCTA CCCACATCGA CTGCTGGACA ATGAGGATGG AGAAAACCCC 
CAGCGGCAGC GCATTAAACG TATTCTCAAG AATTTAGACC AATGGACCAT GCGCCAGTCC 
TCTTTGGAGC TACAGTTGAT GATCAAGCAG ACCCCCAACA CTGAGATGAA CTCTCTCTTG 
GAGAACATTG CCAAGGCCAC AATCGAGGTT TTCCAACAGT CAGCAGAGAC GGGGTCATCT 
TCTGGAAGTA CAGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAACCTGT GCTCAGCTCT 
CTAGAGCGAT CTGGTGTATG GTTGGTGGCT CCTCTCATTG CCAAACTGCC CACTTCAGTC 
CAGGGCCATG TATTAAAAGC TGCTGGGGAG GAACTGGAAA AGGGTCAGCA CCTGGGCTCT 
TCTTCCCGTA AAGAACGAGA TCGACAGAAA CAGAAGAGCA TGTCCCTGTT GAGCCAACAG 
CCTTTCTTAT CACTGGTGCT AACATGTCTG AAAGGACAGG ATGAGCAGCG CGAGGGACTC 
CTGGCCTCCC TCCACAGCCA GGTGCACCAG ATTGTGATTA ATTGGCGAGA AAACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC TGTGCAGCGT AGTACCCAGC AGACTACAGA GTGGGCCCAG 
CTTCTCCTTG AGATCATCAT CAGCGGCACT GTGGACATGC AGTCTAACAA TGAGCTCTTC 
ACTACTGTGT TGGACATGCT GAGCGTGCTT ATCAACGGAA CGTTGGCTGC AGACATGTCC 
AGTATCTCGC AAGGCAGCAT GGAGGAAAAC AAGCGTGCAT ATATGAACCT GGTGAAGAAG 
CTTCAGAAGG ACTTGGGGGA GCGCCAATCA GACAGTCTGG AGAAGGTTCA CCAACTGTTG 
CCACTACCCA AGCAGAACCG AGATGTCATA ACCTGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC TGGCTTCGAT TCCATCTTCA AGAAGGAGGG TCTACAAGTT 
TCTACCAAAC AAAAGATCTC TCCCTGGGAG CTTTTTGAGG GCCTGAAGCC ATCAACAGCA 
CCACTGTCAT GGGCCTGGTT TGGCACAGTC CGAGTGGACC GCAGAGTGGC ACGAGGGGAG 
GAGCAGCAGC GGCTGTTGCT CTATCATACC CACCTGAGGC CTCGACCCAG AGCCTATTAC 
CTGGAACCAC TACCTCTGCC CCCAGAAGAT GAGGAGCCAC CAGCCCCTGC CCTACTAGAG 
CCTGAGAAAA AGGCTCCTGA GCCCCCCAAG ACTGACAAAC CAGGGGCTGC TCCTCCGAGC 
ACTGAGGAGC GCAAAAAGAA GTCTACCAAG GGCAAAAAAC GCAGCCAGCC AGCCACCAAG 
AACGAGGACT ATGGCATGGG GCCAGGTCGG AGTGGCCCCT ATGGTGTGAC AGTGCCTCCA 
GACCTTCTAC ACCATGCAAA TCCTGGTTCT ATATCCCACC TTAGCTACAG GCAAAGCTCC 
ATGGGCCTGT ATACCCAAAA CCAGCCACTA CCTGCTGGTG GCCCTCGTGT GGATCCATAC 
CGCCCCGTGC GATTACCAAT GCAAAAGCTG CCAACTCGAC CAACTTATCC CGGTGTGCTG 
CCTACAACTA TGTCTACTGT CATGGGCCTA GAACCCTCTT CTTATAAGAC ATCTGTATAC 
CGGCAGCAGC AACCCACAGT GCCCCAGGGA CAGCGCCTTC GCCAACAGCT CCAGGCAAAG 
ATACAGAGTC AGGGGATGTT GGGACAGTCA TCTGTCCATC AGATGACCCC TAGTTCTTCC 
TATGGTTTGC AGACTTCCCA GGGCTATACA TCCTATGTTT CTCATGTGGG ATTGCAGCAA 
CACACAGGCC CTGCAGGTAC CATGGTGCCC CCCAGCTACT CCAGCCAGCC TTATCAGAGC 
ACCCACCCTT CTACCAATCC TACTCTTGTA GATCCTACCC GCCACCTGCA ACAGCGGCCC 
AGTGGCTATG TGCATCAGCA GGCCCCAACC TATGGGCATG GACTGACTTC CACTCAAAGG 
TTTTCACACC AGACACTGCA GCAGACACCC ATGATGGGTA CCATGACTCC GTTGAGTGCC 
CAGGGTGTCC AGGCAGGCGT CCGTTCAACT TCCATCCTGC CTGAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAA CAGCAGCAGC AGCAACAACA ACAGCAGCAG 
CAGCAGCAGC AACAACAACA GCAGTACCAT ATCCGACAGC AACAGCAGCA GCAGCAGATG 
CTACGGCAAC AGCAGCAACA ACAGCAACAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAGCAGCCA CACCAGCAGC AGCAGCAGGC AGCTCCTCCC 
CAACCCCAGC CCCAGTCCCA GCCCCAGTTC CAGCGCCAGG GCCTGCAGCA GACCCAGCAG 
CAGCAACAGA CAGCAGCTTT GGTCCGGCAA CTTCAACAAC AGCTCTCTAA TACCCAGCCA 
CAGCCCAGCA CCAACATATT TGGACGCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067496.2
CDS	171..6743
Translation	MAAFGILSYEHRPLKRLRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNPAKISSNFSSIIAEKLRCNTLSDTGRRKSLMNQKDNFWLVTARSQSAINTWFTDLAGTKPLTHLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKMTCAYYAAMSETKVKKKNTADPFTEWTQIITKYLWEQLQKMAEYYRPGPAGSGGCGSTIGPLPHDVEMAIRQWDYNEKLALFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLKLLLPLLLRYSGEFVQSAYLSRRLAYFCTRRLALQLDGVSSHSSHVIAAQSTSSLPTTPAPQPPTSSTPSTPFSDLLMCPQHRPLVFGLSCILQTILLCCPSALVWHYSLTDSRIKTGSPLDHLPIAPSNLPMPEGNSAFTQQVRAKLREIEQQIKERGQAVEVRWSFDKCQEATAGFTIGRVLHTLEVLDSHSFERSDFSNSLDSLCNRIFGLGPSKDGHEISSDDDAVVSLLCEWAVSCKRSGRHRAMVVAKLLEKRQAEIEAERCGESEAADEKGSVASGSLSAPSAPIFQDVLLQFLDTQAPMLTDPRSESERVEFFNLVLLFCELIRHDVFSHNMYTCTLISRGDLAFGAPGPRPPSPFDDPTDDPERKEAEGSSSSKLEDPGLSESMDIDPSSSVLFEDMEKPDFSLFSPTMPCEGKGSPSPEKPDVEKEVKPPAKEKIEGTLGILYDQPRHVQYATHFPIPQEESCSHECNQRLVVLFGVGKQRDDARHAIKKITKDILKVLNRKGTAETDQLAPIVPLNPGDLTFLGGEDGQKRRRNRPEAFPTAEDIFAKFQHLSHYDQHQVTAQVSRNVLEQITSFALGMSYHLPLVQHVQFIFDLMEYSLSISGLIDFAIQLLNELSVVEAELLLKSSDLVGSYTTSLCLCIVAVLRHYHACLILNQDQMAQVFEGLCGVVKHGMNRSDGSSAERCILAYLYDLYTSCSHLKSKFGELFSDFCSKVKNTIYCNVEPSESNMRWAPEFMIDTLENPAAHTFTYTGLGKSLSENPANRYSFVCNALMHVCVGHHDPDRVNDIAILCAELTGYCKSLSAEWLGVLKALCCSSNNGTCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLIRCAAIPSLLNAACSEQDSEPGARLTCRILLHLFKTPQLNPCQSDGNKPTVGIRSSCDRHLLAASQNRIVDGAVFAVLKAVFVLGDAELKGSGFTVPGGTEELPEEEGGGGSSGRRQGGRNISVETASLDVYAKYVLRSICQQEWVGERCLKSLCEDSNDLQDPVLSSAQAQRLMQLICYPHRLLDNEDGENPQRQRIKRILKNLDQWTMRQSSLELQLMIKQTPNTEMNSLLENIAKATIEVFQQSAETGSSSGSTASNMPSSSKTKPVLSSLERSGVWLVAPLIAKLPTSVQGHVLKAAGEELEKGQHLGSSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLASLHSQVHQIVINWRENQYLDDCKPKQLMHEALKLRLNLVGGMFDTVQRSTQQTTEWAQLLLEIIISGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLQKDLGERQSDSLEKVHQLLPLPKQNRDVITCEPQGSLIDTKGNKIAGFDSIFKKEGLQVSTKQKISPWELFEGLKPSTAPLSWAWFGTVRVDRRVARGEEQQRLLLYHTHLRPRPRAYYLEPLPLPPEDEEPPAPALLEPEKKAPEPPKTDKPGAAPPSTEERKKKSTKGKKRSQPATKNEDYGMGPGRSGPYGVTVPPDLLHHANPGSISHLSYRQSSMGLYTQNQPLPAGGPRVDPYRPVRLPMQKLPTRPTYPGVLPTTMSTVMGLEPSSYKTSVYRQQQPTVPQGQRLRQQLQAKIQSQGMLGQSSVHQMTPSSSYGLQTSQGYTSYVSHVGLQQHTGPAGTMVPPSYSSQPYQSTHPSTNPTLVDPTRHLQQRPSGYVHQQAPTYGHGLTSTQRFSHQTLQQTPMMGTMTPLSAQGVQAGVRSTSILPEQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQYHIRQQQQQQQMLRQQQQQQQQQQQQQQQQQQQQQQQQQQQPHQQQQQAAPPQPQPQSQPQFQRQGLQQTQQQQQTAALVRQLQQQLSNTQPQPSTNIFGRY

Target ORF information:

RefSeq Version	NM_021521.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mediator complex subunit 12 (Med12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021521.2

ORF Insert Sequence:

ATGGCGGCTT TCGGGATCTT GAGCTATGAA CACCGACCCC TGAAGCGGCT GCGGCTGGGG 
CCTCCCGATG TGTACCCTCA AGATCCCAAA CAGAAGGAGG ATGAACTGAC GGCTTTGAAT 
GTAAAACAAG GTTTCAATAA CCAGCCTGCT GTCTCTGGGG ACGAACATGG CAGTGCCAAG 
AACGTCAACT TCAATCCTGC CAAGATCAGT TCCAACTTCA GCAGCATCAT CGCAGAGAAG 
TTAAGGTGTA ATACTCTCTC TGACACTGGT CGCAGGAAGT CCCTAATGAA CCAGAAGGAC 
AACTTCTGGC TGGTGACTGC AAGATCTCAG AGTGCTATTA ACACCTGGTT CACTGACCTG 
GCTGGCACCA AACCACTCAC ACACCTAGCC AAAAAGGTCC CCATTTTCAG TAAAAAGGAA 
GAAGTATTTG GGTATTTGGC CAAATACACG GTGCCTGTGA TGCGGGCTGC CTGGCTCATT 
AAGATGACTT GTGCCTACTA TGCAGCAATG TCTGAGACTA AGGTTAAGAA GAAAAATACT 
GCTGACCCCT TCACTGAATG GACTCAAATC ATCACAAAGT ACTTGTGGGA GCAGCTACAG 
AAGATGGCTG AGTACTATCG GCCAGGGCCT GCAGGAAGTG GAGGCTGTGG TTCTACTATA 
GGGCCTTTGC CCCATGATGT AGAGATGGCC ATCAGGCAGT GGGATTACAA TGAGAAGCTC 
GCCTTGTTCA TGTTTCAGGA TGGAATGCTG GACAGACATG AGTTCCTGAC TTGGGTGCTT 
GAGTGTTTTG AGAAAATACG CCCTGGAGAA GATGAATTGC TTAAATTGCT GCTTCCCCTA 
CTGCTGCGAT ACTCAGGGGA GTTTGTTCAG TCTGCCTATC TGTCCCGCCG CCTTGCCTAC 
TTCTGTACCC GGAGATTGGC TCTCCAGCTG GATGGTGTGA GCAGCCACTC ATCTCATGTC 
ATAGCTGCTC AGTCAACAAG TTCTCTGCCC ACTACCCCAG CACCTCAGCC CCCAACTAGC 
AGTACACCCT CGACTCCCTT TAGTGACCTG CTTATGTGCC CTCAGCATCG TCCCCTGGTT 
TTTGGCCTCA GCTGTATCCT TCAGACCATC CTTCTGTGTT GCCCTAGTGC CCTAGTTTGG 
CACTACTCAT TGACTGATAG CCGAATTAAG ACCGGCTCAC CACTTGACCA CCTGCCTATT 
GCTCCTTCCA ACTTGCCCAT GCCAGAGGGT AACAGTGCCT TTACTCAGCA GGTCCGTGCA 
AAATTACGAG AGATCGAACA ACAGATCAAG GAGCGTGGAC AAGCAGTTGA GGTTCGCTGG 
TCTTTTGATA AGTGCCAGGA AGCTACTGCA GGTTTCACCA TTGGACGGGT GCTCCATACT 
TTAGAAGTGC TGGATAGCCA TAGTTTCGAG CGCTCTGACT TTAGTAACTC TCTTGACTCC 
CTTTGTAATC GAATCTTTGG ATTGGGGCCT AGTAAGGATG GTCATGAGAT TTCCTCTGAT 
GATGATGCTG TGGTATCATT ATTGTGTGAA TGGGCTGTAA GCTGCAAACG CTCAGGTCGG 
CATCGTGCTA TGGTAGTAGC CAAACTCCTA GAGAAGAGAC AAGCAGAAAT TGAGGCTGAG 
CGCTGCGGAG AATCTGAAGC AGCTGATGAG AAGGGCTCCG TCGCCTCTGG TTCCCTTTCC 
GCTCCTAGTG CACCCATTTT CCAGGATGTC CTCCTGCAGT TTCTGGATAC ACAGGCTCCC 
ATGCTGACTG ATCCCCGAAG TGAAAGTGAG CGAGTAGAAT TCTTTAACTT GGTACTGCTC 
TTCTGTGAAC TGATCCGACA TGATGTCTTC TCCCATAATA TGTACACCTG CACTCTCATC 
TCTCGGGGGG ATCTTGCATT TGGAGCCCCT GGTCCTCGGC CTCCCTCTCC CTTTGATGAT 
CCTACAGATG ATCCAGAGCG CAAGGAGGCT GAAGGCAGCA GCAGCAGCAA GCTAGAGGAC 
CCAGGGCTCT CTGAATCTAT GGACATCGAC CCTAGTTCCA GTGTGCTTTT TGAGGACATG 
GAAAAGCCTG ATTTCTCATT GTTCTCCCCT ACTATGCCTT GTGAGGGGAA GGGAAGCCCA 
TCCCCTGAGA AACCAGATGT CGAAAAGGAA GTAAAACCCC CAGCCAAAGA GAAGATCGAG 
GGGACACTTG GGATTCTGTA TGACCAGCCA CGACATGTGC AGTATGCCAC ACACTTTCCA 
ATCCCACAGG AGGAGTCATG CAGCCATGAG TGCAACCAGC GGTTGGTCGT ACTGTTTGGG 
GTGGGGAAGC AGCGAGATGA TGCCCGCCAT GCCATCAAGA AGATTACCAA GGATATCCTG 
AAGGTTCTGA ATCGCAAGGG GACAGCAGAA ACTGACCAGC TTGCTCCTAT TGTGCCTCTG 
AATCCTGGAG ACCTGACATT CTTAGGTGGG GAAGATGGGC AGAAGCGACG ACGCAACCGG 
CCCGAAGCCT TCCCCACTGC TGAAGATATT TTTGCTAAGT TCCAGCACCT TTCCCATTAT 
GACCAACACC AGGTCACGGC TCAGGTCTCC CGGAACGTTC TGGAGCAGAT CACGAGCTTC 
GCCCTTGGCA TGTCGTACCA CTTGCCTCTG GTGCAGCATG TGCAGTTCAT CTTCGACCTC 
ATGGAATATT CACTGAGCAT CAGTGGCCTC ATCGACTTTG CCATCCAGTT ACTGAATGAG 
CTGAGTGTGG TTGAGGCGGA GCTCCTTCTC AAATCGTCGG ATCTGGTGGG CAGCTACACT 
ACCAGCCTGT GCTTATGTAT CGTGGCTGTC CTTCGACACT ATCATGCCTG CCTCATCCTC 
AATCAGGACC AGATGGCGCA AGTGTTTGAG GGGCTCTGTG GCGTGGTGAA ACATGGAATG 
AACCGTTCAG ATGGCTCCTC TGCAGAGCGC TGTATCCTTG CTTATCTCTA TGATCTGTAT 
ACCTCCTGTA GCCATTTAAA GAGCAAATTT GGGGAGCTCT TCAGTGACTT TTGCTCAAAA 
GTGAAGAACA CCATCTACTG TAACGTGGAG CCATCGGAAT CCAATATGCG CTGGGCACCG 
GAGTTCATGA TTGACACTCT GGAGAACCCT GCCGCTCACA CTTTCACCTA CACGGGGCTC 
GGCAAGAGTC TTAGTGAGAA CCCTGCTAAC CGCTATAGCT TTGTGTGCAA TGCTCTTATG 
CACGTCTGCG TGGGCCACCA TGATCCTGAT AGGGTAAATG ACATCGCCAT CCTTTGTGCA 
GAGCTGACCG GCTATTGCAA GTCCTTGAGT GCTGAGTGGC TAGGAGTACT TAAGGCCTTG 
TGCTGCTCCT CGAACAATGG CACTTGTGGT TTCAATGATC TCCTGTGCAA CGTAGACGTC 
AGTGATTTGT CTTTTCATGA TTCCTTGGCT ACTTTTGTTG CTATCCTCAT CGCCCGTCAA 
TGTTTGCTCC TAGAAGACCT GATTCGCTGT GCAGCCATCC CTTCACTCCT CAATGCTGCT 
TGTAGTGAGC AGGATTCTGA GCCAGGAGCC AGGCTTACTT GCCGAATCCT CCTCCACCTG 
TTCAAGACGC CACAACTCAA TCCTTGCCAG TCTGATGGAA ACAAACCTAC TGTTGGAATC 
CGGTCCTCCT GTGACCGCCA CCTGCTGGCT GCCTCCCAGA ACCGCATCGT GGATGGGGCT 
GTGTTTGCTG TTCTCAAGGC TGTGTTTGTA CTCGGAGATG CGGAGCTAAA AGGTTCAGGC 
TTTACTGTGC CGGGAGGAAC AGAAGAACTT CCAGAAGAGG AGGGAGGAGG TGGTAGTAGC 
GGTCGGAGAC AGGGTGGCCG CAACATCTCT GTGGAGACAG CAAGTCTGGA TGTCTATGCC 
AAGTACGTGC TGCGAAGCAT CTGCCAACAG GAATGGGTAG GAGAACGTTG CCTTAAGTCA 
CTGTGTGAGG ATAGCAATGA TCTACAAGAC CCAGTGTTGA GTAGTGCCCA GGCCCAGCGC 
CTCATGCAAC TTATCTGCTA CCCACATCGA CTGCTGGACA ATGAGGATGG AGAAAACCCC 
CAGCGGCAGC GCATTAAACG TATTCTCAAG AATTTAGACC AATGGACCAT GCGCCAGTCC 
TCTTTGGAGC TACAGTTGAT GATCAAGCAG ACCCCCAACA CTGAGATGAA CTCTCTCTTG 
GAGAACATTG CCAAGGCCAC AATCGAGGTT TTCCAACAGT CAGCAGAGAC GGGGTCATCT 
TCTGGAAGTA CAGCAAGCAA CATGCCCAGC AGCAGCAAGA CCAAACCTGT GCTCAGCTCT 
CTAGAGCGAT CTGGTGTATG GTTGGTGGCT CCTCTCATTG CCAAACTGCC CACTTCAGTC 
CAGGGCCATG TATTAAAAGC TGCTGGGGAG GAACTGGAAA AGGGTCAGCA CCTGGGCTCT 
TCTTCCCGTA AAGAACGAGA TCGACAGAAA CAGAAGAGCA TGTCCCTGTT GAGCCAACAG 
CCTTTCTTAT CACTGGTGCT AACATGTCTG AAAGGACAGG ATGAGCAGCG CGAGGGACTC 
CTGGCCTCCC TCCACAGCCA GGTGCACCAG ATTGTGATTA ATTGGCGAGA AAACCAGTAC 
TTAGATGATT GCAAACCAAA GCAGCTAATG CATGAGGCAC TCAAACTGCG GCTCAACCTG 
GTGGGGGGCA TGTTTGACAC TGTGCAGCGT AGTACCCAGC AGACTACAGA GTGGGCCCAG 
CTTCTCCTTG AGATCATCAT CAGCGGCACT GTGGACATGC AGTCTAACAA TGAGCTCTTC 
ACTACTGTGT TGGACATGCT GAGCGTGCTT ATCAACGGAA CGTTGGCTGC AGACATGTCC 
AGTATCTCGC AAGGCAGCAT GGAGGAAAAC AAGCGTGCAT ATATGAACCT GGTGAAGAAG 
CTTCAGAAGG ACTTGGGGGA GCGCCAATCA GACAGTCTGG AGAAGGTTCA CCAACTGTTG 
CCACTACCCA AGCAGAACCG AGATGTCATA ACCTGTGAGC CACAGGGCTC CCTTATTGAC 
ACCAAGGGCA ACAAGATTGC TGGCTTCGAT TCCATCTTCA AGAAGGAGGG TCTACAAGTT 
TCTACCAAAC AAAAGATCTC TCCCTGGGAG CTTTTTGAGG GCCTGAAGCC ATCAACAGCA 
CCACTGTCAT GGGCCTGGTT TGGCACAGTC CGAGTGGACC GCAGAGTGGC ACGAGGGGAG 
GAGCAGCAGC GGCTGTTGCT CTATCATACC CACCTGAGGC CTCGACCCAG AGCCTATTAC 
CTGGAACCAC TACCTCTGCC CCCAGAAGAT GAGGAGCCAC CAGCCCCTGC CCTACTAGAG 
CCTGAGAAAA AGGCTCCTGA GCCCCCCAAG ACTGACAAAC CAGGGGCTGC TCCTCCGAGC 
ACTGAGGAGC GCAAAAAGAA GTCTACCAAG GGCAAAAAAC GCAGCCAGCC AGCCACCAAG 
AACGAGGACT ATGGCATGGG GCCAGGTCGG AGTGGCCCCT ATGGTGTGAC AGTGCCTCCA 
GACCTTCTAC ACCATGCAAA TCCTGGTTCT ATATCCCACC TTAGCTACAG GCAAAGCTCC 
ATGGGCCTGT ATACCCAAAA CCAGCCACTA CCTGCTGGTG GCCCTCGTGT GGATCCATAC 
CGCCCCGTGC GATTACCAAT GCAAAAGCTG CCAACTCGAC CAACTTATCC CGGTGTGCTG 
CCTACAACTA TGTCTACTGT CATGGGCCTA GAACCCTCTT CTTATAAGAC ATCTGTATAC 
CGGCAGCAGC AACCCACAGT GCCCCAGGGA CAGCGCCTTC GCCAACAGCT CCAGGCAAAG 
ATACAGAGTC AGGGGATGTT GGGACAGTCA TCTGTCCATC AGATGACCCC TAGTTCTTCC 
TATGGTTTGC AGACTTCCCA GGGCTATACA TCCTATGTTT CTCATGTGGG ATTGCAGCAA 
CACACAGGCC CTGCAGGTAC CATGGTGCCC CCCAGCTACT CCAGCCAGCC TTATCAGAGC 
ACCCACCCTT CTACCAATCC TACTCTTGTA GATCCTACCC GCCACCTGCA ACAGCGGCCC 
AGTGGCTATG TGCATCAGCA GGCCCCAACC TATGGGCATG GACTGACTTC CACTCAAAGG 
TTTTCACACC AGACACTGCA GCAGACACCC ATGATGGGTA CCATGACTCC GTTGAGTGCC 
CAGGGTGTCC AGGCAGGCGT CCGTTCAACT TCCATCCTGC CTGAGCAGCA GCAACAACAA 
CAGCAGCAGC AGCAACAGCA GCAGCAGCAA CAGCAGCAGC AGCAACAACA ACAGCAGCAG 
CAGCAGCAGC AACAACAACA GCAGTACCAT ATCCGACAGC AACAGCAGCA GCAGCAGATG 
CTACGGCAAC AGCAGCAACA ACAGCAACAG CAGCAGCAGC AGCAACAGCA GCAGCAGCAA 
CAACAACAGC AACAGCAGCA GCAGCAGCCA CACCAGCAGC AGCAGCAGGC AGCTCCTCCC 
CAACCCCAGC CCCAGTCCCA GCCCCAGTTC CAGCGCCAGG GCCTGCAGCA GACCCAGCAG 
CAGCAACAGA CAGCAGCTTT GGTCCGGCAA CTTCAACAAC AGCTCTCTAA TACCCAGCCA 
CAGCCCAGCA CCAACATATT TGGACGCTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MED12 regulates a transcriptional network of calcium-handling genes in the heart.
JCI insight2(14)(2017 Jul)
Baskin KK,Makarewich CA,DeLeon SM,Ye W,Chen B,Beetz N,Schrewe H,Bassel-Duby R,Olson EN

Med12 regulates ovarian steroidogenesis, uterine development and maternal effects in the mammalian egg.
Biology of reproduction97(6)822-834(2017 Jan)
Wang X,Mittal P,Castro CA,Rajkovic G,Rajkovic A

MED12 Regulates HSC-Specific Enhancers Independently of Mediator Kinase Activity to Control Hematopoiesis.
Cell stem cell19(6)784-799(2016 Dec)
Aranda-Orgilles B,Salda?a-Meyer R,Wang E,Trompouki E,Fassl A,Lau S,Mullenders J,Rocha PP,Raviram R,Guillamot M,S?nchez-D?az M,Wang K,Kayembe C,Zhang N,Amoasii L,Choudhuri A,Skok JA,Schober M,Reinberg D,Sicinski P,Schrewe H,Tsirigos A,Zon LI,Aifantis I

Dual role of Med12 in PRC1-dependent gene repression and ncRNA-mediated transcriptional activation.
Cell cycle (Georgetown, Tex.)15(11)1479-93(2016 Jun)
Papadopoulou T,Kaymak A,Sayols S,Richly H

Mediator subunit Med12 contributes to the maintenance of neural stem cell identity.
BMC developmental biology16(1)17(2016 05)
Kim NH,Livi CB,Yew PR,Boyer TG