Crbn cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crbn
Entrez Gene ID	58799
Full Name	cereblon
Synonyms	2610203G15Rik,2900045O07Rik,AF229032,AW108261,piL
General protein information	Preferred Names cereblon Names protein cereblon novel lethal protein PiL
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6\|6 E1
Summary	This gene encodes a protein with a Lon protease domain, a 'regulators of G protein-signaling' (RGS)-like domain and a leucine zipper. It has been proposed to regulate the assembly and surface expression of large-conductance calcium-activated potassium channels in brain and to bind thalidomide. In humans mutation in this gene causes autosomal recessive nonsyndromic cognitive disability. In mouse deficiency of this gene serves as a model to study the molecular mechanisms governing learning and memory as they relate to intellectual disability. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021449.3	NP_067424.2	protein cereblon isoform 1
NM_175357.3	NP_780566.1	protein cereblon isoform 2

Pathways from BioSystems

Homologies

	CRBN	XP_001100581.2
Bos taurus (cattle)	CRBN	NP_001068995.1
Mus musculus (house mouse)	Crbn	NP_067424.2
Gallus gallus (chicken)	CRBN	XP_004944767.1
Homo sapiens (human)	CRBN	NP_001166953.1
Oryza sativa (rice)	Os05g0521500	NP_001056076.1
Pan troglodytes (chimpanzee)	CRBN	XP_001140433.1
Canis lupus familiaris (dog)	CRBN	XP_533757.2
Danio rerio (zebrafish)	crbn	NP_001003996.1
Arabidopsis thaliana (thale cress)	AT2G25740	NP_850069.1
Oryza sativa (rice)	Os01g0743600	NP_001044220.1
Rattus norvegicus (Norway rat)	Crbn	NP_001015003.1
Xenopus tropicalis (tropical clawed frog)	crbn	NP_001008192.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005299	XP_309090.4

Gene Ontology

The following Crbn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crbn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu05220	NM_175357.3 Latest version!	Mus musculus cereblon (Crbn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu12361	NM_021449.3 Latest version!	Mus musculus cereblon (Crbn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crbn ( NM_175357.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_780566.1 Mus musculus cereblon (Crbn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu05220
Clone ID Related Accession (Same CDS sequence)	NM_175357.3
Accession Version	NM_175357.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	protein cereblon isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ073661.1, BQ749260.1 and BC046967.1. On Jan 17, 2013 this sequence version replaced NM_175357.2. Transcript Variant: This variant (2) uses an alternate in-frame acceptor splice site in the coding region compared to variant 1. It encodes isoform 2 which is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046967.1, SRR1660821.26529.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCG AGGGAGATCA GCAGGACGCT GCGCACAACA TGGGAAACCA CCTGCCGCTT 
CTGCCTGCAG ACAGTGAAGA TGAAGATGAT GAAATTGAAA TGGAAGTTGA AGACCAAGAT 
AGTAAAGAAG CCAGAAAACC GAATATCATA AACTTTGACA CCAGTCTGCC AACCTCACAT 
ACATACCTGG GAGCTGATAT GGAGGAGTTC CACGGGAGAA CTTTGCATGA CGACGACAGC 
TGCCAGGTGA TCCCAGTCCT TCCTGAGGTG CTGATGATCC TGATTCCTGG GCAGACACTC 
CCACTGCAGC TCTCTCACCC ACAGGAAGTC AGCATGGTGC GGAACTTAAT CCAGAAAGAC 
AGGACCTTTG CAGTCCTTGC ATACAGTAAT GTGCAAGAAA GGGAAGCACA GTTTGGGACA 
ACAGCAGAGA TCTATGCCTA TCGAGAAGAG CAGGAGTTTG GAATTGAAGT AGTGAAAGTG 
AAAGCAATTG GAAGGCAGCG GTTCAAGGTC CTCGAACTTC GAACACAGTC AGATGGAATC 
CAGCAAGCTA AAGTGCAGAT TTTGCCAGAG TGTGTGTTGC CGTCAACCAT GTCTGCAGTG 
CAGTTAGAAT CACTCAATAA GTGCCAGGTA TTTCCTTCAA AACCCATCTC CTGGGAAGAC 
CAGTATTCAT GTAAATGGTG GCAGAAATAC CAGAAGAGAA AGTTTCACTG TGCAAATCTA 
ACATCATGGC CTCGCTGGCT GTATTCATTA TATGATGCTG AAACATTAAT GGATAGAATT 
AAGAAACAGC TACGTGAATG GGATGAAAAT CTCAAAGATG ATTCTCTTCC TGAAAATCCA 
ATAGACTTTT CTTACAGAGT AGCTGCTTGT CTTCCTATTG ATGATGTATT GAGAATTCAG 
CTCCTTAAAA TCGGCAGTGC TATTCAACGG CTTCGCTGTG AATTGGACAT CATGAACAAA 
TGTACTTCCC TTTGCTGTAA ACAATGTCAA GAAACAGAAA TAACGACAAA GAATGAAATA 
TTTAGTTTAT CCTTATGTGG TCCAATGGCA GCATATGTGA ATCCTCATGG ATATGTACAT 
GAGACACTGA CTGTGTATAA AGCGTCCAAC CTGAATCTGA TAGGCCGGCC TTCTACAGTG 
CACAGCTGGT TTCCCGGGTA TGCATGGACC ATTGCCCAGT GCAAGATCTG TGCAAGCCAT 
ATTGGATGGA AATTTACAGC CACAAAAAAA GACATGTCAC CTCAAAAATT TTGGGGCTTA 
ACTCGCTCTG CTCTGTTACC CACAATTCCA GAGACTGAAG ATGAAATAAG TCCAGACAAA 
GTAATACTTT GTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_780566.1
CDS	31..1368
Translation	MAGEGDQQDAAHNMGNHLPLLPADSEDEDDEIEMEVEDQDSKEARKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPEVLMILIPGQTLPLQLSHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQEFGIEVVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQVFPSKPISWEDQYSCKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPENPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPETEDEISPDKVILCL

Target ORF information:

RefSeq Version	NM_175357.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cereblon (Crbn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175357.3

ORF Insert Sequence:

ATGGCCGGCG AGGGAGATCA GCAGGACGCT GCGCACAACA TGGGAAACCA CCTGCCGCTT 
CTGCCTGCAG ACAGTGAAGA TGAAGATGAT GAAATTGAAA TGGAAGTTGA AGACCAAGAT 
AGTAAAGAAG CCAGAAAACC GAATATCATA AACTTTGACA CCAGTCTGCC AACCTCACAT 
ACATACCTGG GAGCTGATAT GGAGGAGTTC CACGGGAGAA CTTTGCATGA CGACGACAGC 
TGCCAGGTGA TCCCAGTCCT TCCTGAGGTG CTGATGATCC TGATTCCTGG GCAGACACTC 
CCACTGCAGC TCTCTCACCC ACAGGAAGTC AGCATGGTGC GGAACTTAAT CCAGAAAGAC 
AGGACCTTTG CAGTCCTTGC ATACAGTAAT GTGCAAGAAA GGGAAGCACA GTTTGGGACA 
ACAGCAGAGA TCTATGCCTA TCGAGAAGAG CAGGAGTTTG GAATTGAAGT AGTGAAAGTG 
AAAGCAATTG GAAGGCAGCG GTTCAAGGTC CTCGAACTTC GAACACAGTC AGATGGAATC 
CAGCAAGCTA AAGTGCAGAT TTTGCCAGAG TGTGTGTTGC CGTCAACCAT GTCTGCAGTG 
CAGTTAGAAT CACTCAATAA GTGCCAGGTA TTTCCTTCAA AACCCATCTC CTGGGAAGAC 
CAGTATTCAT GTAAATGGTG GCAGAAATAC CAGAAGAGAA AGTTTCACTG TGCAAATCTA 
ACATCATGGC CTCGCTGGCT GTATTCATTA TATGATGCTG AAACATTAAT GGATAGAATT 
AAGAAACAGC TACGTGAATG GGATGAAAAT CTCAAAGATG ATTCTCTTCC TGAAAATCCA 
ATAGACTTTT CTTACAGAGT AGCTGCTTGT CTTCCTATTG ATGATGTATT GAGAATTCAG 
CTCCTTAAAA TCGGCAGTGC TATTCAACGG CTTCGCTGTG AATTGGACAT CATGAACAAA 
TGTACTTCCC TTTGCTGTAA ACAATGTCAA GAAACAGAAA TAACGACAAA GAATGAAATA 
TTTAGTTTAT CCTTATGTGG TCCAATGGCA GCATATGTGA ATCCTCATGG ATATGTACAT 
GAGACACTGA CTGTGTATAA AGCGTCCAAC CTGAATCTGA TAGGCCGGCC TTCTACAGTG 
CACAGCTGGT TTCCCGGGTA TGCATGGACC ATTGCCCAGT GCAAGATCTG TGCAAGCCAT 
ATTGGATGGA AATTTACAGC CACAAAAAAA GACATGTCAC CTCAAAAATT TTGGGGCTTA 
ACTCGCTCTG CTCTGTTACC CACAATTCCA GAGACTGAAG ATGAAATAAG TCCAGACAAA 
GTAATACTTT GTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crbn ( NM_021449.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067424.2 Mus musculus cereblon (Crbn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12361
Clone ID Related Accession (Same CDS sequence)	NM_021449.3
Accession Version	NM_021449.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-07
Organism	Mus musculus(house mouse)
Product	protein cereblon isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CJ073661.1, AK160219.1 and BC046967.1. On Jan 17, 2013 this sequence version replaced NM_021449.2. Transcript Variant: This variant (1) represents the shorter transcript and encodes the shorter isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK160219.1, BC086488.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGGCG AGGGAGATCA GCAGGACGCT GCGCACAACA TGGGAAACCA CCTGCCGCTT 
CTGCCTGACA GTGAAGATGA AGATGATGAA ATTGAAATGG AAGTTGAAGA CCAAGATAGT 
AAAGAAGCCA GAAAACCGAA TATCATAAAC TTTGACACCA GTCTGCCAAC CTCACATACA 
TACCTGGGAG CTGATATGGA GGAGTTCCAC GGGAGAACTT TGCATGACGA CGACAGCTGC 
CAGGTGATCC CAGTCCTTCC TGAGGTGCTG ATGATCCTGA TTCCTGGGCA GACACTCCCA 
CTGCAGCTCT CTCACCCACA GGAAGTCAGC ATGGTGCGGA ACTTAATCCA GAAAGACAGG 
ACCTTTGCAG TCCTTGCATA CAGTAATGTG CAAGAAAGGG AAGCACAGTT TGGGACAACA 
GCAGAGATCT ATGCCTATCG AGAAGAGCAG GAGTTTGGAA TTGAAGTAGT GAAAGTGAAA 
GCAATTGGAA GGCAGCGGTT CAAGGTCCTC GAACTTCGAA CACAGTCAGA TGGAATCCAG 
CAAGCTAAAG TGCAGATTTT GCCAGAGTGT GTGTTGCCGT CAACCATGTC TGCAGTGCAG 
TTAGAATCAC TCAATAAGTG CCAGGTATTT CCTTCAAAAC CCATCTCCTG GGAAGACCAG 
TATTCATGTA AATGGTGGCA GAAATACCAG AAGAGAAAGT TTCACTGTGC AAATCTAACA 
TCATGGCCTC GCTGGCTGTA TTCATTATAT GATGCTGAAA CATTAATGGA TAGAATTAAG 
AAACAGCTAC GTGAATGGGA TGAAAATCTC AAAGATGATT CTCTTCCTGA AAATCCAATA 
GACTTTTCTT ACAGAGTAGC TGCTTGTCTT CCTATTGATG ATGTATTGAG AATTCAGCTC 
CTTAAAATCG GCAGTGCTAT TCAACGGCTT CGCTGTGAAT TGGACATCAT GAACAAATGT 
ACTTCCCTTT GCTGTAAACA ATGTCAAGAA ACAGAAATAA CGACAAAGAA TGAAATATTT 
AGTTTATCCT TATGTGGTCC AATGGCAGCA TATGTGAATC CTCATGGATA TGTACATGAG 
ACACTGACTG TGTATAAAGC GTCCAACCTG AATCTGATAG GCCGGCCTTC TACAGTGCAC 
AGCTGGTTTC CCGGGTATGC ATGGACCATT GCCCAGTGCA AGATCTGTGC AAGCCATATT 
GGATGGAAAT TTACAGCCAC AAAAAAAGAC ATGTCACCTC AAAAATTTTG GGGCTTAACT 
CGCTCTGCTC TGTTACCCAC AATTCCAGAG ACTGAAGATG AAATAAGTCC AGACAAAGTA 
ATACTTTGTT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067424.2
CDS	31..1365
Translation	MAGEGDQQDAAHNMGNHLPLLPDSEDEDDEIEMEVEDQDSKEARKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPEVLMILIPGQTLPLQLSHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQEFGIEVVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQVFPSKPISWEDQYSCKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPENPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKASNLNLIGRPSTVHSWFPGYAWTIAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPETEDEISPDKVILCL

Target ORF information:

RefSeq Version	NM_021449.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cereblon (Crbn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021449.3

ORF Insert Sequence:

ATGGCCGGCG AGGGAGATCA GCAGGACGCT GCGCACAACA TGGGAAACCA CCTGCCGCTT 
CTGCCTGACA GTGAAGATGA AGATGATGAA ATTGAAATGG AAGTTGAAGA CCAAGATAGT 
AAAGAAGCCA GAAAACCGAA TATCATAAAC TTTGACACCA GTCTGCCAAC CTCACATACA 
TACCTGGGAG CTGATATGGA GGAGTTCCAC GGGAGAACTT TGCATGACGA CGACAGCTGC 
CAGGTGATCC CAGTCCTTCC TGAGGTGCTG ATGATCCTGA TTCCTGGGCA GACACTCCCA 
CTGCAGCTCT CTCACCCACA GGAAGTCAGC ATGGTGCGGA ACTTAATCCA GAAAGACAGG 
ACCTTTGCAG TCCTTGCATA CAGTAATGTG CAAGAAAGGG AAGCACAGTT TGGGACAACA 
GCAGAGATCT ATGCCTATCG AGAAGAGCAG GAGTTTGGAA TTGAAGTAGT GAAAGTGAAA 
GCAATTGGAA GGCAGCGGTT CAAGGTCCTC GAACTTCGAA CACAGTCAGA TGGAATCCAG 
CAAGCTAAAG TGCAGATTTT GCCAGAGTGT GTGTTGCCGT CAACCATGTC TGCAGTGCAG 
TTAGAATCAC TCAATAAGTG CCAGGTATTT CCTTCAAAAC CCATCTCCTG GGAAGACCAG 
TATTCATGTA AATGGTGGCA GAAATACCAG AAGAGAAAGT TTCACTGTGC AAATCTAACA 
TCATGGCCTC GCTGGCTGTA TTCATTATAT GATGCTGAAA CATTAATGGA TAGAATTAAG 
AAACAGCTAC GTGAATGGGA TGAAAATCTC AAAGATGATT CTCTTCCTGA AAATCCAATA 
GACTTTTCTT ACAGAGTAGC TGCTTGTCTT CCTATTGATG ATGTATTGAG AATTCAGCTC 
CTTAAAATCG GCAGTGCTAT TCAACGGCTT CGCTGTGAAT TGGACATCAT GAACAAATGT 
ACTTCCCTTT GCTGTAAACA ATGTCAAGAA ACAGAAATAA CGACAAAGAA TGAAATATTT 
AGTTTATCCT TATGTGGTCC AATGGCAGCA TATGTGAATC CTCATGGATA TGTACATGAG 
ACACTGACTG TGTATAAAGC GTCCAACCTG AATCTGATAG GCCGGCCTTC TACAGTGCAC 
AGCTGGTTTC CCGGGTATGC ATGGACCATT GCCCAGTGCA AGATCTGTGC AAGCCATATT 
GGATGGAAAT TTACAGCCAC AAAAAAAGAC ATGTCACCTC AAAAATTTTG GGGCTTAACT 
CGCTCTGCTC TGTTACCCAC AATTCCAGAG ACTGAAGATG AAATAAGTCC AGACAAAGTA 
ATACTTTGTT TATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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Crbn cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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FAX

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