Miox cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Miox
Entrez Gene ID	56727
Full Name	myo-inositol oxygenase
Synonyms	0610009I10Rik,AI314022,Aldrl6,C85427,RSOR
General protein information	Preferred Names myo-inositol oxygenase Names inositol oxygenase MI oxygenase aldehyde reductase (aldose reductase)-like 6 aldehyde reductase 6, renal aldehyde reductase-like 6 renal-specific oxido-reducatse renal-specific oxidoreductase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 E3

mRNA and Protein(s)

mRNA	Protein	Name
NM_019977.2	NP_064361.2	inositol oxygenase

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MIOX	NP_001094535.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003636	XP_313400.3
Caenorhabditis elegans (roundworm)	C54E4.5	NP_500170.3
Oryza sativa (rice)	Os06g0561000	NP_001057871.1
Xenopus tropicalis (tropical clawed frog)	miox	XP_002939418.2
	MIOX	XP_001116334.1
Drosophila melanogaster (fruit fly)	CG6910	NP_648556.3
Danio rerio (zebrafish)	miox	NP_001025437.1
Mus musculus (house mouse)	Miox	NP_064361.2
Eremothecium gossypii	AGOS_AFR482W	NP_986029.1
Magnaporthe oryzae (rice blast fungus)	MGG_09462	XP_003712291.1
Arabidopsis thaliana (thale cress)	MIOX1	NP_172904.2
Homo sapiens (human)	MIOX	NP_060054.4
Canis lupus familiaris (dog)	MIOX	XP_538310.3
Rattus norvegicus (Norway rat)	Miox	NP_665714.2
Kluyveromyces lactis	KLLA0E10737g	XP_454435.1
Neurospora crassa	NCU08856	XP_960986.1

Gene Ontology
Bibliography

Related articles in PubMed

Beneficial Effects of
Dutta RK, Kondeti VK, Sharma I, Chandel NS, Quaggin SE, Kanwar YS
Journal of the American Society of Nephrology : JASN28(5)1421-1436(2017 May)

High Glucose-Induced Hypomethylation Promotes Binding of Sp-1 to Myo-Inositol Oxygenase: Implication in the Pathobiology of Diabetic Tubulopathy.
Sharma I, Dutta RK, Singh NK, Kanwar YS
The American journal of pathology187(4)724-739(2017 Apr)

Transcriptional and Translational Modulation of myo-Inositol Oxygenase (Miox) by Fatty Acids: IMPLICATIONS IN RENAL TUBULAR INJURY INDUCED IN OBESITY AND DIABETES.
Tominaga T, Dutta RK, Joladarashi D, Doi T, Reddy JK, Kanwar YS
The Journal of biological chemistry291(3)1348-67(2016 Jan)

Disruption of renal tubular mitochondrial quality control by Myo-inositol oxygenase in diabetic kidney disease.
Zhan M, Usman IM, Sun L, Kanwar YS
Journal of the American Society of Nephrology : JASN26(6)1304-21(2015 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings highlight the epigenetic regulation of MIOX in the pathogenesis of diabetic tubulopathy.
Title: High Glucose-Induced Hypomethylation Promotes Binding of Sp-1 to Myo-Inositol Oxygenase: Implication in the Pathobiology of Diabetic Tubulopathy.

MIOX under high glucose ambience exacerbates renal injury during the progression of diabetic nephropathy following the generation of excessive reactive oxygen species via an unexplored glucuronate-xylulose pathway.
Title: myo-Inositol Oxygenase Overexpression Accentuates Generation of Reactive Oxygen Species and Exacerbates Cellular Injury following High Glucose Ambience: A NEW MECHANISM RELEVANT TO THE PATHOGENESIS OF DIABETIC NEPHROPATHY.

Data suggest that Miox expression/activity is up-regulated in kidneys tubules during oxidative stress (as in obesity and diabetic nephropathy); up-regulation of Miox activity is related to phosphorylation of serine/threonine residues.
Title: Transcriptional and Translational Modulation of myo-Inositol Oxygenase (Miox) by Fatty Acids: IMPLICATIONS IN RENAL TUBULAR INJURY INDUCED IN OBESITY AND DIABETES.

MIOX expression attenuated tubular damage and improved renal function.
Title: Disruption of renal tubular mitochondrial quality control by Myo-inositol oxygenase in diabetic kidney disease.

KSP-positive cells acquired the characteristics of each segment of renal tubular cells through tubular formation when stimulated with Wnt4.
Title: Kidney specific protein-positive cells derived from embryonic stem cells reproduce tubular structures in vitro and differentiate into renal tubular cells.

The following Miox gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Miox cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu11863	NM_019977.2 Latest version!	Mus musculus myo-inositol oxygenase (Miox), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Miox ( NM_019977.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_064361.2 Mus musculus myo-inositol oxygenase (Miox), mRNA.

Price & Availability↑

CloneID	OMu11863
Clone ID Related Accession (Same CDS sequence)	NM_019977.2
Accession Version	NM_019977.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	inositol oxygenase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC013543.1. On Mar 17, 2004 this sequence version replaced NM_019977.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013543.1, AF197127.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGTCG ATGTGGGCCC AGACCCTTCC CTGGTCTATC GACCCGATGT GGACCCAGAG 
ATGGCCAAAA GCAAGGACAG CTTCCGAAAC TATACTTCAG GCCCGCTGCT GGATCGTGTC 
TTTACCACAT ACAAGCTCAT GCACACTCAC CAGACTGTGG ACTTCGTCAG CAGGAAGCGC 
ATCCAGTATG GAAGCTTCTC TTACAAGAAG ATGACCATCA TGGAGGCTGT GGGCATGCTG 
GATGATCTGG TGGACGAATC TGACCCAGAC GTAGATTTCC CCAACTCCTT CCACGCGTTC 
CAGACCGCGG AGGGCATCCG GAAAGCCCAC CCGGACAAGG ACTGGTTCCA CCTGGTCGGA 
CTTTTGCACG ATCTGGGGAA AATTATGGCT CTGTGGGGGG AACCTCAGTG GGCTGTTGTT 
GGAGACACGT TCCCCGTGGG CTGCCGTCCC CAGGCCTCTG TGGTGTTCTG TGACTCTACT 
TTCCAGGACA ATCCTGACCT CCAGGATCCT CGATACAGCA CAGAACTCGG CATGTACCAG 
CCTCACTGTG GACTAGAGAA CGTCCTTATG TCCTGGGGCC ATGATGAGTA CCTATACCAG 
ATGATGAAGT TCAACAAGTT CTCCCTGCCT TCAGAGGCCT TCTACATGAT CCGATTCCAC 
TCCTTCTATC CGTGGCACAC CGGCGGTGAC TACCGGCAGC TGTGCAGCCA GCAGGACCTG 
GATATGCTGC CCTGGGTGCA AGAGTTCAAC AAGTTTGATC TCTACACGAA GTGCCCTGAC 
CTACCGGATG TGGAGAGCCT GCGGCCCTAC TATCAAGGGC TGATTGACAA GTACTGCCCG 
GGCACCCTGA GCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_064361.2
CDS	31..888
Translation	MKVDVGPDPSLVYRPDVDPEMAKSKDSFRNYTSGPLLDRVFTTYKLMHTHQTVDFVSRKRIQYGSFSYKKMTIMEAVGMLDDLVDESDPDVDFPNSFHAFQTAEGIRKAHPDKDWFHLVGLLHDLGKIMALWGEPQWAVVGDTFPVGCRPQASVVFCDSTFQDNPDLQDPRYSTELGMYQPHCGLENVLMSWGHDEYLYQMMKFNKFSLPSEAFYMIRFHSFYPWHTGGDYRQLCSQQDLDMLPWVQEFNKFDLYTKCPDLPDVESLRPYYQGLIDKYCPGTLSW

Target ORF information:

RefSeq Version	NM_019977.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus myo-inositol oxygenase (Miox), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019977.2

ORF Insert Sequence:

ATGAAGGTCG ATGTGGGCCC AGACCCTTCC CTGGTCTATC GACCCGATGT GGACCCAGAG 
ATGGCCAAAA GCAAGGACAG CTTCCGAAAC TATACTTCAG GCCCGCTGCT GGATCGTGTC 
TTTACCACAT ACAAGCTCAT GCACACTCAC CAGACTGTGG ACTTCGTCAG CAGGAAGCGC 
ATCCAGTATG GAAGCTTCTC TTACAAGAAG ATGACCATCA TGGAGGCTGT GGGCATGCTG 
GATGATCTGG TGGACGAATC TGACCCAGAC GTAGATTTCC CCAACTCCTT CCACGCGTTC 
CAGACCGCGG AGGGCATCCG GAAAGCCCAC CCGGACAAGG ACTGGTTCCA CCTGGTCGGA 
CTTTTGCACG ATCTGGGGAA AATTATGGCT CTGTGGGGGG AACCTCAGTG GGCTGTTGTT 
GGAGACACGT TCCCCGTGGG CTGCCGTCCC CAGGCCTCTG TGGTGTTCTG TGACTCTACT 
TTCCAGGACA ATCCTGACCT CCAGGATCCT CGATACAGCA CAGAACTCGG CATGTACCAG 
CCTCACTGTG GACTAGAGAA CGTCCTTATG TCCTGGGGCC ATGATGAGTA CCTATACCAG 
ATGATGAAGT TCAACAAGTT CTCCCTGCCT TCAGAGGCCT TCTACATGAT CCGATTCCAC 
TCCTTCTATC CGTGGCACAC CGGCGGTGAC TACCGGCAGC TGTGCAGCCA GCAGGACCTG 
GATATGCTGC CCTGGGTGCA AGAGTTCAAC AAGTTTGATC TCTACACGAA GTGCCCTGAC 
CTACCGGATG TGGAGAGCCT GCGGCCCTAC TATCAAGGGC TGATTGACAA GTACTGCCCG 
GGCACCCTGA GCTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Beneficial Effects of
Journal of the American Society of Nephrology : JASN28(5)1421-1436(2017 May)
Dutta RK,Kondeti VK,Sharma I,Chandel NS,Quaggin SE,Kanwar YS

High Glucose-Induced Hypomethylation Promotes Binding of Sp-1 to Myo-Inositol Oxygenase: Implication in the Pathobiology of Diabetic Tubulopathy.
The American journal of pathology187(4)724-739(2017 Apr)
Sharma I,Dutta RK,Singh NK,Kanwar YS

Transcriptional and Translational Modulation of myo-Inositol Oxygenase (Miox) by Fatty Acids: IMPLICATIONS IN RENAL TUBULAR INJURY INDUCED IN OBESITY AND DIABETES.
The Journal of biological chemistry291(3)1348-67(2016 Jan)
Tominaga T,Dutta RK,Joladarashi D,Doi T,Reddy JK,Kanwar YS

Disruption of renal tubular mitochondrial quality control by Myo-inositol oxygenase in diabetic kidney disease.
Journal of the American Society of Nephrology : JASN26(6)1304-21(2015 Jun)
Zhan M,Usman IM,Sun L,Kanwar YS