Sae1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sae1
Entrez Gene ID	56459
Full Name	SUMO1 activating enzyme subunit 1
Synonyms	2400010M20Rik,2610044L12Rik,AL033372,AOS1,AW743391,D7Ertd177e,HSPC140,Sua1,Uble1a
General protein information	Preferred Names SUMO1 activating enzyme subunit 1 Names SUMO-activating enzyme subunit 1 SUMO-1 activating enzyme subunit 1 ubiquitin-like (sentrin) activating enzyme E1A ubiquitin-like 1 (sentrin) activating enzyme E1A ubiquitin-like 1 (sentrin) activating enzyme subunit 1 ubiquitin-like 1-activating enzyme E1A
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 A2\|7 8.88 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001285891.1	NP_001272820.1	SUMO-activating enzyme subunit 1 isoform a
XM_011250653.1	XP_011248955.1	SUMO-activating enzyme subunit 1 isoform X1
NM_001285892.1	NP_001272821.1	SUMO-activating enzyme subunit 1 isoform b
NM_019748.3	NP_062722.1	SUMO-activating enzyme subunit 1 isoform a

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SAE1	NP_001075180.1
Mus musculus (house mouse)	Sae1	NP_001272820.1
Drosophila melanogaster (fruit fly)	Aos1	NP_650198.1
Saccharomyces cerevisiae (baker's yeast)	AOS1	NP_015506.1
Schizosaccharomyces pombe (fission yeast)	rad31	NP_593251.1
Magnaporthe oryzae (rice blast fungus)	MGG_01669	XP_003714655.1
Arabidopsis thaliana (thale cress)	SAE1A	NP_567712.1
Xenopus tropicalis (tropical clawed frog)	sae1	NP_001016870.1
Oryza sativa (rice)	Os11g0497000	NP_001067937.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004459	XP_003436824.1
Kluyveromyces lactis	KLLA0E04863g	XP_002999394.1
Neurospora crassa	NCU09006	XP_959034.1
Arabidopsis thaliana (thale cress)	SAE1B	NP_851162.1
Homo sapiens (human)	SAE1	NP_005491.1
Pan troglodytes (chimpanzee)	SAE1	NP_001233548.1
Canis lupus familiaris (dog)	SAE1	XP_533632.1
Caenorhabditis elegans (roundworm)	aos-1	NP_505604.1
Rattus norvegicus (Norway rat)	Sae1	NP_001012063.1
Danio rerio (zebrafish)	sae1	NP_001002058.1
Arabidopsis thaliana (thale cress)	SAE1B	NP_001032050.1

Gene Ontology
Bibliography

Related articles in PubMed

Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis.
Ban R, Nishida T, Urano T
Genes to cells : devoted to molecular & cellular mechanisms16(6)652-69(2011 Jun)

Three-dimensional visualization of testis cord morphogenesis, a novel tubulogenic mechanism in development.
Combes AN, Lesieur E, Harley VR, Sinclair AH, Little MH, Wilhelm D, Koopman P
Developmental dynamics : an official publication of the American Association of Anatomists238(5)1033-41(2009 May)

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Stryke D, Kawamoto M, Huang CC, Johns SJ, King LA, Harper CA, Meng EC, Lee RE, Yee A, L'Italien L, Chuang PT, Young SG, Skarnes WC, Babbitt PC, Ferrin TE
Nucleic acids research31(1)278-81(2003 Jan)

Molecular features of human ubiquitin-like SUMO genes and their encoded proteins.
Su HL, Li SS
Gene296(1-2)65-73(2002 Aug)

Expression and regulation of the mammalian SUMO-1 E1 enzyme.
Azuma Y, Tan SH, Cavenagh MM, Ainsztein AM, Saitoh H, Dasso M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology15(10)1825-7(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sae1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sae1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16425	NM_001285892.1 Latest version!	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu69656	XM_011250653.1 Latest version!	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu16397	NM_019748.3 Latest version!	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
OMu16397	NM_001285891.1 Latest version!	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sae1 ( NM_001285892.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272821.1 Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu16425
Clone ID Related Accession (Same CDS sequence)	NM_001285892.1
Accession Version	NM_001285892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	SUMO-activating enzyme subunit 1 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB838763.1, AC164880.3, AK011772.1 and AK162789.1. Transcript Variant: This variant (3) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (b) has a shorter and distinct C-terminus compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: AK162789.1, AK011772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGTGCTG CTGAAATTCC GCACAGATAA AGGGAGAGAT 
CCGACTTCTG AGAGCTACAA GGAGGATGCG GAGCTGCTGC TGCAGATACG GAATGACGTG 
TTTGACTCCC TGGGCATCAG TCCTGACCTG CTTCCCGATG ACTTTGTCAG GTACTGCTTC 
TCTGAGATGG CCCCAGTATG TGCTGTGGTT GGAGGGATCT TGGCACAGGA AATTGTGAAG 
GTTTGTCTGG GCACCATGTG TGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272821.1
CDS	147..1133
Translation	MVEKEEAGGGGGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDTEDVEKKPESFFTKFDAVCLTCCSRDVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQVLLKFRTDKGRDPTSESYKEDAELLLQIRNDVFDSLGISPDLLPDDFVRYCFSEMAPVCAVVGGILAQEIVKVCLGTMCV

Target ORF information:

RefSeq Version	NM_001285892.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285892.1

ORF Insert Sequence:

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGTGCTG CTGAAATTCC GCACAGATAA AGGGAGAGAT 
CCGACTTCTG AGAGCTACAA GGAGGATGCG GAGCTGCTGC TGCAGATACG GAATGACGTG 
TTTGACTCCC TGGGCATCAG TCCTGACCTG CTTCCCGATG ACTTTGTCAG GTACTGCTTC 
TCTGAGATGG CCCCAGTATG TGCTGTGGTT GGAGGGATCT TGGCACAGGA AATTGTGAAG 
GTTTGTCTGG GCACCATGTG TGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sae1 ( XM_011250653.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248955.1 Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu69656
Clone ID Related Accession (Same CDS sequence)	XM_011250653.1
Accession Version	XM_011250653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	SUMO-activating enzyme subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGGTACT GCTTCTCTGA GATGGCCCCA GTATGTGCTG 
TGGTTGGAGG GATCTTGGCA CAGGAAATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248955.1
CDS	142..954
Translation	MVEKEEAGGGGGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDTEDVEKKPESFFTKFDAVCLTCCSRDVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQGTASLRWPQYVLWLEGSWHRKL

Target ORF information:

RefSeq Version	XM_011250653.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250653.1

ORF Insert Sequence:

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGGTACT GCTTCTCTGA GATGGCCCCA GTATGTGCTG 
TGGTTGGAGG GATCTTGGCA CAGGAAATTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sae1 ( NM_001285891.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001272820.1 Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16397
Clone ID Related Accession (Same CDS sequence)	NM_001285891.1 , NM_019748.3
Accession Version	NM_001285891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	SUMO-activating enzyme subunit 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB838763.1, AC164880.3, BC068164.1 and AK162789.1. Transcript Variant: This variant (2) differs in the 3' UTR compared to variant 1. Variants 1 and 2 both encode the same isoform (a). ##Evidence-Data-START## Transcript exon combination :: AK154139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END##

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGTGCTG CTGAAATTCC GCACAGATAA AGGGAGAGAT 
CCGACTTCTG AGAGCTACAA GGAGGATGCG GAGCTGCTGC TGCAGATACG GAATGACGTG 
TTTGACTCCC TGGGCATCAG TCCTGACCTG CTTCCCGATG ACTTTGTCAG GTACTGCTTC 
TCTGAGATGG CCCCAGTATG TGCTGTGGTT GGAGGGATCT TGGCACAGGA AATTGTGAAG 
GCCCTGTCTC AAAGGGACCC TCCTCACAAC AACTTCTTCT TCTTCGACGG CATGAAGGGG 
AGTGGAATTG TGGAGTGCCT TGGTCCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272820.1
CDS	147..1199
Translation	MVEKEEAGGGGGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDTEDVEKKPESFFTKFDAVCLTCCSRDVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQVLLKFRTDKGRDPTSESYKEDAELLLQIRNDVFDSLGISPDLLPDDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVECLGPQ

Target ORF information:

RefSeq Version	NM_001285891.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001285891.1

ORF Insert Sequence:

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGTGCTG CTGAAATTCC GCACAGATAA AGGGAGAGAT 
CCGACTTCTG AGAGCTACAA GGAGGATGCG GAGCTGCTGC TGCAGATACG GAATGACGTG 
TTTGACTCCC TGGGCATCAG TCCTGACCTG CTTCCCGATG ACTTTGTCAG GTACTGCTTC 
TCTGAGATGG CCCCAGTATG TGCTGTGGTT GGAGGGATCT TGGCACAGGA AATTGTGAAG 
GCCCTGTCTC AAAGGGACCC TCCTCACAAC AACTTCTTCT TCTTCGACGG CATGAAGGGG 
AGTGGAATTG TGGAGTGCCT TGGTCCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sae1 ( NM_019748.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062722.1 Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16397
Clone ID Related Accession (Same CDS sequence)	NM_001285891.1 , NM_019748.3
Accession Version	NM_019748.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Mus musculus(house mouse)
Product	SUMO-activating enzyme subunit 1 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB838763.1, AC164880.3 and BC068164.1. On Oct 24, 2013 this sequence version replaced NM_019748.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode the same isoform (a). ##Evidence-Data-START## Transcript exon combination :: BC068164.1, SRR1660819.168739.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849382 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGTGCTG CTGAAATTCC GCACAGATAA AGGGAGAGAT 
CCGACTTCTG AGAGCTACAA GGAGGATGCG GAGCTGCTGC TGCAGATACG GAATGACGTG 
TTTGACTCCC TGGGCATCAG TCCTGACCTG CTTCCCGATG ACTTTGTCAG GTACTGCTTC 
TCTGAGATGG CCCCAGTATG TGCTGTGGTT GGAGGGATCT TGGCACAGGA AATTGTGAAG 
GCCCTGTCTC AAAGGGACCC TCCTCACAAC AACTTCTTCT TCTTCGACGG CATGAAGGGG 
AGTGGAATTG TGGAGTGCCT TGGTCCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062722.1
CDS	147..1199
Translation	MVEKEEAGGGGGGGISEEEAAQYDRQIRLWGLEAQKRLRASRVLIVGMKGLGAEIAKNLILAGVKGLTMLDHEQVSPEDPGAQFLIQTGSVGRNRAEASLERAQNLNPMVDVKVDTEDVEKKPESFFTKFDAVCLTCCSRDVIIKVDQICHRNSIKFFTGDVFGYHGYTFANLGEHEFVEEKTKVAKVSQGVEDGPEAKRAKLDSSETTMVKKKVLFCPVKEALEVDWSGEKAKAALKRTAPDYFLLQVLLKFRTDKGRDPTSESYKEDAELLLQIRNDVFDSLGISPDLLPDDFVRYCFSEMAPVCAVVGGILAQEIVKALSQRDPPHNNFFFFDGMKGSGIVECLGPQ

Target ORF information:

RefSeq Version	NM_019748.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus SUMO1 activating enzyme subunit 1 (Sae1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019748.3

ORF Insert Sequence:

ATGGTAGAGA AGGAGGAGGC TGGCGGCGGC GGCGGCGGTG GCATCAGCGA GGAGGAGGCA 
GCGCAGTATG ACCGACAGAT CCGCCTGTGG GGACTGGAGG CCCAGAAGCG GCTTCGAGCT 
TCCCGGGTGC TGATTGTTGG CATGAAAGGA CTTGGAGCTG AAATTGCCAA GAATCTTATC 
CTGGCAGGAG TCAAAGGGCT CACCATGCTG GACCACGAAC AGGTATCTCC AGAAGACCCT 
GGAGCTCAGT TCTTGATTCA GACTGGGTCT GTGGGCCGCA ATAGGGCCGA GGCCTCTTTG 
GAACGAGCCC AGAATCTCAA TCCCATGGTG GATGTAAAGG TGGACACTGA GGATGTAGAG 
AAGAAGCCAG AGTCCTTCTT CACGAAGTTT GATGCGGTGT GCCTGACTTG CTGCTCCAGA 
GATGTCATCA TTAAAGTCGA CCAGATCTGT CACAGAAACA GCATTAAGTT CTTCACAGGA 
GATGTCTTTG GGTACCATGG GTACACCTTT GCGAATCTTG GAGAGCATGA ATTTGTAGAA 
GAGAAAACCA AAGTTGCCAA AGTAAGCCAA GGAGTGGAAG ATGGACCTGA GGCCAAGAGA 
GCAAAGCTTG ATTCATCAGA GACAACCATG GTCAAAAAGA AAGTGCTTTT TTGCCCTGTA 
AAAGAGGCGC TAGAAGTGGA CTGGAGTGGA GAGAAAGCCA AGGCTGCCCT GAAGCGTACT 
GCCCCCGACT ACTTTCTGCT GCAAGTGCTG CTGAAATTCC GCACAGATAA AGGGAGAGAT 
CCGACTTCTG AGAGCTACAA GGAGGATGCG GAGCTGCTGC TGCAGATACG GAATGACGTG 
TTTGACTCCC TGGGCATCAG TCCTGACCTG CTTCCCGATG ACTTTGTCAG GTACTGCTTC 
TCTGAGATGG CCCCAGTATG TGCTGTGGTT GGAGGGATCT TGGCACAGGA AATTGTGAAG 
GCCCTGTCTC AAAGGGACCC TCCTCACAAC AACTTCTTCT TCTTCGACGG CATGAAGGGG 
AGTGGAATTG TGGAGTGCCT TGGTCCCCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mitotic kinase Aurora-B is regulated by SUMO-2/3 conjugation/deconjugation during mitosis.
Genes to cells : devoted to molecular & cellular mechanisms16(6)652-69(2011 Jun)
Ban R,Nishida T,Urano T

Three-dimensional visualization of testis cord morphogenesis, a novel tubulogenic mechanism in development.
Developmental dynamics : an official publication of the American Association of Anatomists238(5)1033-41(2009 May)
Combes AN,Lesieur E,Harley VR,Sinclair AH,Little MH,Wilhelm D,Koopman P

BayGenomics: a resource of insertional mutations in mouse embryonic stem cells.
Nucleic acids research31(1)278-81(2003 Jan)
Stryke D,Kawamoto M,Huang CC,Johns SJ,King LA,Harper CA,Meng EC,Lee RE,Yee A,L'Italien L,Chuang PT,Young SG,Skarnes WC,Babbitt PC,Ferrin TE

Molecular features of human ubiquitin-like SUMO genes and their encoded proteins.
Gene296(1-2)65-73(2002 Aug)
Su HL,Li SS

Expression and regulation of the mammalian SUMO-1 E1 enzyme.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology15(10)1825-7(2001 Aug)
Azuma Y,Tan SH,Cavenagh MM,Ainsztein AM,Saitoh H,Dasso M

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Sae1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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