Letm1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Letm1
Entrez Gene ID	56384
Full Name	leucine zipper-EF-hand containing transmembrane protein 1
General protein information	Preferred Names leucine zipper-EF-hand containing transmembrane protein 1 Names mitochondrial proton/calcium exchanger protein LETM1 and EF-hand domain-containing protein 1, mitochondrial
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B2

mRNA and Protein(s)

mRNA	Protein	Name
NM_019694.1	NP_062668.1	mitochondrial proton/calcium exchanger protein precursor
NM_019694.2	NP_062668.1	mitochondrial proton/calcium exchanger protein precursor
XM_006504034.3	XP_006504097.1	LETM1 and EF-hand domain-containing protein 1, mitochondrial isoform X1
XM_006504034.4	XP_006504097.1	mitochondrial proton/calcium exchanger protein isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LETM1	NP_001069082.1
Mus musculus (house mouse)	Letm1	NP_062668.1
Danio rerio (zebrafish)	letm1	NP_001038673.1
Kluyveromyces lactis	KLLA0B11341g	XP_452034.1
Neurospora crassa	NCU03217	XP_965317.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003296	XP_003436436.1
Caenorhabditis elegans (roundworm)	letm-1	NP_506381.1
Saccharomyces cerevisiae (baker's yeast)	YLH47	NP_015450.1
Eremothecium gossypii	AGOS_AAR032W	NP_982573.2
Xenopus tropicalis (tropical clawed frog)	letm1	NP_001072793.1
Canis lupus familiaris (dog)	LETM1	XP_545925.2
Rattus norvegicus (Norway rat)	Letm1	NP_001005884.1
Magnaporthe oryzae (rice blast fungus)	MGG_03684	XP_003716197.1
Homo sapiens (human)	LETM1	NP_036450.1
Gallus gallus (chicken)	LETM1	NP_001006461.2
Pan troglodytes (chimpanzee)	LETM1	XP_003310276.1
	LETM1	XP_002802216.1
Schizosaccharomyces pombe (fission yeast)	mdm28	NP_593648.1

Gene Ontology
Bibliography

Related articles in PubMed

PINK1-mediated phosphorylation of LETM1 regulates mitochondrial calcium transport and protects neurons against mitochondrial stress.
Huang E, Qu D, Huang T, Rizzi N, Boonying W, Krolak D, Ciana P, Woulfe J, Klein C, Slack RS, Figeys D, Park DS
Nature communications8(1)1399(2017 Nov)

Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) forms a Ca
Shao J, Fu Z, Ji Y, Guan X, Guo S, Ding Z, Yang X, Cong Y, Shen Y
Scientific reports634174(2016 Sep)

LETM1-dependent mitochondrial Ca2+ flux modulates cellular bioenergetics and proliferation.
Doonan PJ, Chandramoorthy HC, Hoffman NE, Zhang X, C?rdenas C, Shanmughapriya S, Rajan S, Vallem S, Chen X, Foskett JK, Cheung JY, Houser SR, Madesh M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(11)4936-49(2014 Nov)

Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.
Jiang D, Zhao L, Clish CB, Clapham DE
Proceedings of the National Academy of Sciences of the United States of America110(24)E2249-54(2013 Jun)

Endoplasmic reticulum potassium-hydrogen exchanger and small conductance calcium-activated potassium channel activities are essential for ER calcium uptake in neurons and cardiomyocytes.
Kuum M, Veksler V, Liiv J, Ventura-Clapier R, Kaasik A
Journal of cell science125(Pt 3)625-33(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Reconstitution of LETM1 or antioxidant overexpression rescued mitochondrial Ca(2+) transport and bioenergetics
Title: LETM1-dependent mitochondrial Ca2+ flux modulates cellular bioenergetics and proliferation.

Data indicate that cellular Letm1 knockdown reduced Ca(2+)mito uptake, H(+)mito extrusion and impaired mitochondrial ATP generation capacity.
Title: Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.

The following Letm1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Letm1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu35144	XM_006504034.4 Latest version!	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OMu14659	NM_019694.2 Latest version!	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$433.30 $619.00
View Old Accession Versions >>
OMu14659	NM_019694.1	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$433.30 $619.00
OMu35144	XM_006504034.3	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Letm1 ( XM_006504034.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504097.1 Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35144
Clone ID Related Accession (Same CDS sequence)	XM_006504034.3 , XM_006504034.4
Accession Version	XM_006504034.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	LETM1 and EF-hand domain-containing protein 1, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGGC GCATCCTCAA TGGCCATACG CTGACCCGCC GGGAGCGCAG GCAGTTTCTC 
CGGATTTGTG CGGACCTCTT CCGTCTAGTG CCCTTCCTGG TGTTCGTGGT GGTGCCCTTC 
ATGGAATTCC TGCTGCCTGT AGTTGTGAAG CTCTTCCCCA ACATGCTGCC CTCTACATTT 
GAGACCCAGT CTATCAAGGA GGAAAGGCTG AAGAAGGAGC TGAGGGTGAA GCTGGAGCTG 
GCCAAGTTTC TCCAGGACAC CATAGAGGAA ATGGCCCTGA AGAACAAGGC AGCCAAAGGG 
AATGCCACCA AAGATTTCTC TGCATTTTTC CAGAAGATCC GGGAGACAGG GGAGAGACCC 
AGCAATGAGG AAATCATGCG TTTTTCCAAA CTGTTTGAGG ATGAACTGAC CCTGGATAAC 
CTCACGAGGC CTCAGCTGGT AGCACTGTGC AAGCTGCTGG AGCTTCAGTC CATTGGCACC 
AACAACTTCC TGCGTTTCCA GCTCACCATG CGCCTGAGGT CCATAAAGGC TGATGATAAG 
CTGATTTCTG AGGAAGGGGT GGACAGTCTG ACTGTAAAAG AATTGCAGGC AGCATGTCGA 
GCACGGGGCA TGAGAGCTCT CGGTGTCACA GAAGACCGTC TGAAGGGCCA GCTGAAACAG 
TGGCTGGACT TGCACCTGCA TCACGAGATC CCCACATCAT TGCTCATACT GTCCCGGGCC 
ATGTACCTCC CAGACACCCT CTCACCTGCT GACCAGCTGA AGTCCACCTT GCAGACCCTT 
CCAGAAATTG TGGCAAAGGA GGCCCAGGTG AAAGTGGCCG AGGTGGAGGG CGAAAAGGTA 
GACAACAAGG CGAAGCTGGA GGCCACACTG CAGGAAGAGG CCGCCATCCA GCAAGAACAC 
CTGGAAGAAC TAAAGAGAGC CTCTGAGGCT GTGAAGGACA TCCAGCCAGA AGTAGCGGAA 
GCCACTCTCC CTGGGAGGCC AGGGCCTGAG CCTCAGCCTC CAGTGGATGA TGTGATCCTG 
CCCTCAGAGG TCCTGACGGA TACGGCACCT GTACTGGAAG GTCTGAAGGG TGAAGAGATT 
ACCAAGGAGG AGATTGACAT CCTCAGTGAC GCCTGCTCCA AGCTGCAGGA GCAGAAGAAG 
TCCCTGACCA AGGAGAAGGA GGAGCTGGAG CTGCTCAAAG AGGACGTCCA GGACTACAGT 
GAGGACTTAC AGGAGATCAA GAAGGAACTT TCAAAGACGG GTGAGGAGAA GTACATAGAA 
GAATCTGCAG CGAGCAAGAG GCTGTCAAAG CGAGTGCAGC AGATGATCGG GCAGATCGAT 
GGCCTGATCA CACAGCTGGA GACCACTCAA CAGGATGGCA AGCTGGGCCC CAGCCAGAGC 
ACTCCAACAG GGGAGAGTGT CATCAGCATC ACTGAGCTCA TCAGCGCCAT GAAGCAAATC 
AAGCACATTC CAGAGCACAA GCTCATCAGT TTGACCTCAG CCCTGGATGA TAATAAGGAC 
GGCAATATCA ACATCGATGA CCTTGTCAAG GTAATCGACT TGGTGAACAA AGAAGATGTT 
CAGATCTCTA CCACCCAGGT AGCTGAAATT GTGGCCACAC TAGAGAAAGA AGAAAAGATA 
GAAGAGAAAG AGAAGGCCAA GGAGAAGGCT GAGAAAGAGG CCGCAGAAGT GAAGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504097.1
CDS	729..2408
Translation	MLWRILNGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVVVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSAFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLISEEGVDSLTVKELQAACRARGMRALGVTEDRLKGQLKQWLDLHLHHEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEKVDNKAKLEATLQEEAAIQQEHLEELKRASEAVKDIQPEVAEATLPGRPGPEPQPPVDDVILPSEVLTDTAPVLEGLKGEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYIEESAASKRLSKRVQQMIGQIDGLITQLETTQQDGKLGPSQSTPTGESVISITELISAMKQIKHIPEHKLISLTSALDDNKDGNINIDDLVKVIDLVNKEDVQISTTQVAEIVATLEKEEKIEEKEKAKEKAEKEAAEVKN

Target ORF information:

RefSeq Version	XM_006504034.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504034.3

ORF Insert Sequence:

ATGCTCTGGC GCATCCTCAA TGGCCATACG CTGACCCGCC GGGAGCGCAG GCAGTTTCTC 
CGGATTTGTG CGGACCTCTT CCGTCTAGTG CCCTTCCTGG TGTTCGTGGT GGTGCCCTTC 
ATGGAATTCC TGCTGCCTGT AGTTGTGAAG CTCTTCCCCA ACATGCTGCC CTCTACATTT 
GAGACCCAGT CTATCAAGGA GGAAAGGCTG AAGAAGGAGC TGAGGGTGAA GCTGGAGCTG 
GCCAAGTTTC TCCAGGACAC CATAGAGGAA ATGGCCCTGA AGAACAAGGC AGCCAAAGGG 
AATGCCACCA AAGATTTCTC TGCATTTTTC CAGAAGATCC GGGAGACAGG GGAGAGACCC 
AGCAATGAGG AAATCATGCG TTTTTCCAAA CTGTTTGAGG ATGAACTGAC CCTGGATAAC 
CTCACGAGGC CTCAGCTGGT AGCACTGTGC AAGCTGCTGG AGCTTCAGTC CATTGGCACC 
AACAACTTCC TGCGTTTCCA GCTCACCATG CGCCTGAGGT CCATAAAGGC TGATGATAAG 
CTGATTTCTG AGGAAGGGGT GGACAGTCTG ACTGTAAAAG AATTGCAGGC AGCATGTCGA 
GCACGGGGCA TGAGAGCTCT CGGTGTCACA GAAGACCGTC TGAAGGGCCA GCTGAAACAG 
TGGCTGGACT TGCACCTGCA TCACGAGATC CCCACATCAT TGCTCATACT GTCCCGGGCC 
ATGTACCTCC CAGACACCCT CTCACCTGCT GACCAGCTGA AGTCCACCTT GCAGACCCTT 
CCAGAAATTG TGGCAAAGGA GGCCCAGGTG AAAGTGGCCG AGGTGGAGGG CGAAAAGGTA 
GACAACAAGG CGAAGCTGGA GGCCACACTG CAGGAAGAGG CCGCCATCCA GCAAGAACAC 
CTGGAAGAAC TAAAGAGAGC CTCTGAGGCT GTGAAGGACA TCCAGCCAGA AGTAGCGGAA 
GCCACTCTCC CTGGGAGGCC AGGGCCTGAG CCTCAGCCTC CAGTGGATGA TGTGATCCTG 
CCCTCAGAGG TCCTGACGGA TACGGCACCT GTACTGGAAG GTCTGAAGGG TGAAGAGATT 
ACCAAGGAGG AGATTGACAT CCTCAGTGAC GCCTGCTCCA AGCTGCAGGA GCAGAAGAAG 
TCCCTGACCA AGGAGAAGGA GGAGCTGGAG CTGCTCAAAG AGGACGTCCA GGACTACAGT 
GAGGACTTAC AGGAGATCAA GAAGGAACTT TCAAAGACGG GTGAGGAGAA GTACATAGAA 
GAATCTGCAG CGAGCAAGAG GCTGTCAAAG CGAGTGCAGC AGATGATCGG GCAGATCGAT 
GGCCTGATCA CACAGCTGGA GACCACTCAA CAGGATGGCA AGCTGGGCCC CAGCCAGAGC 
ACTCCAACAG GGGAGAGTGT CATCAGCATC ACTGAGCTCA TCAGCGCCAT GAAGCAAATC 
AAGCACATTC CAGAGCACAA GCTCATCAGT TTGACCTCAG CCCTGGATGA TAATAAGGAC 
GGCAATATCA ACATCGATGA CCTTGTCAAG GTAATCGACT TGGTGAACAA AGAAGATGTT 
CAGATCTCTA CCACCCAGGT AGCTGAAATT GTGGCCACAC TAGAGAAAGA AGAAAAGATA 
GAAGAGAAAG AGAAGGCCAA GGAGAAGGCT GAGAAAGAGG CCGCAGAAGT GAAGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Letm1 ( XM_006504034.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504097.1 Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35144
Clone ID Related Accession (Same CDS sequence)	XM_006504034.3 , XM_006504034.4
Accession Version	XM_006504034.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1680bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitochondrial proton/calcium exchanger protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504034.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCTGGC GCATCCTCAA TGGCCATACG CTGACCCGCC GGGAGCGCAG GCAGTTTCTC 
CGGATTTGTG CGGACCTCTT CCGTCTAGTG CCCTTCCTGG TGTTCGTGGT GGTGCCCTTC 
ATGGAATTCC TGCTGCCTGT AGTTGTGAAG CTCTTCCCCA ACATGCTGCC CTCTACATTT 
GAGACCCAGT CTATCAAGGA GGAAAGGCTG AAGAAGGAGC TGAGGGTGAA GCTGGAGCTG 
GCCAAGTTTC TCCAGGACAC CATAGAGGAA ATGGCCCTGA AGAACAAGGC AGCCAAAGGG 
AATGCCACCA AAGATTTCTC TGCATTTTTC CAGAAGATCC GGGAGACAGG GGAGAGACCC 
AGCAATGAGG AAATCATGCG TTTTTCCAAA CTGTTTGAGG ATGAACTGAC CCTGGATAAC 
CTCACGAGGC CTCAGCTGGT AGCACTGTGC AAGCTGCTGG AGCTTCAGTC CATTGGCACC 
AACAACTTCC TGCGTTTCCA GCTCACCATG CGCCTGAGGT CCATAAAGGC TGATGATAAG 
CTGATTTCTG AGGAAGGGGT GGACAGTCTG ACTGTAAAAG AATTGCAGGC AGCATGTCGA 
GCACGGGGCA TGAGAGCTCT CGGTGTCACA GAAGACCGTC TGAAGGGCCA GCTGAAACAG 
TGGCTGGACT TGCACCTGCA TCACGAGATC CCCACATCAT TGCTCATACT GTCCCGGGCC 
ATGTACCTCC CAGACACCCT CTCACCTGCT GACCAGCTGA AGTCCACCTT GCAGACCCTT 
CCAGAAATTG TGGCAAAGGA GGCCCAGGTG AAAGTGGCCG AGGTGGAGGG CGAAAAGGTA 
GACAACAAGG CGAAGCTGGA GGCCACACTG CAGGAAGAGG CCGCCATCCA GCAAGAACAC 
CTGGAAGAAC TAAAGAGAGC CTCTGAGGCT GTGAAGGACA TCCAGCCAGA AGTAGCGGAA 
GCCACTCTCC CTGGGAGGCC AGGGCCTGAG CCTCAGCCTC CAGTGGATGA TGTGATCCTG 
CCCTCAGAGG TCCTGACGGA TACGGCACCT GTACTGGAAG GTCTGAAGGG TGAAGAGATT 
ACCAAGGAGG AGATTGACAT CCTCAGTGAC GCCTGCTCCA AGCTGCAGGA GCAGAAGAAG 
TCCCTGACCA AGGAGAAGGA GGAGCTGGAG CTGCTCAAAG AGGACGTCCA GGACTACAGT 
GAGGACTTAC AGGAGATCAA GAAGGAACTT TCAAAGACGG GTGAGGAGAA GTACATAGAA 
GAATCTGCAG CGAGCAAGAG GCTGTCAAAG CGAGTGCAGC AGATGATCGG GCAGATCGAT 
GGCCTGATCA CACAGCTGGA GACCACTCAA CAGGATGGCA AGCTGGGCCC CAGCCAGAGC 
ACTCCAACAG GGGAGAGTGT CATCAGCATC ACTGAGCTCA TCAGCGCCAT GAAGCAAATC 
AAGCACATTC CAGAGCACAA GCTCATCAGT TTGACCTCAG CCCTGGATGA TAATAAGGAC 
GGCAATATCA ACATCGATGA CCTTGTCAAG GTAATCGACT TGGTGAACAA AGAAGATGTT 
CAGATCTCTA CCACCCAGGT AGCTGAAATT GTGGCCACAC TAGAGAAAGA AGAAAAGATA 
GAAGAGAAAG AGAAGGCCAA GGAGAAGGCT GAGAAAGAGG CCGCAGAAGT GAAGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504097.1
CDS	728..2407
Translation	MLWRILNGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVVVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSAFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLISEEGVDSLTVKELQAACRARGMRALGVTEDRLKGQLKQWLDLHLHHEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEKVDNKAKLEATLQEEAAIQQEHLEELKRASEAVKDIQPEVAEATLPGRPGPEPQPPVDDVILPSEVLTDTAPVLEGLKGEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYIEESAASKRLSKRVQQMIGQIDGLITQLETTQQDGKLGPSQSTPTGESVISITELISAMKQIKHIPEHKLISLTSALDDNKDGNINIDDLVKVIDLVNKEDVQISTTQVAEIVATLEKEEKIEEKEKAKEKAEKEAAEVKN

Target ORF information:

RefSeq Version	XM_006504034.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504034.4

ORF Insert Sequence:

ATGCTCTGGC GCATCCTCAA TGGCCATACG CTGACCCGCC GGGAGCGCAG GCAGTTTCTC 
CGGATTTGTG CGGACCTCTT CCGTCTAGTG CCCTTCCTGG TGTTCGTGGT GGTGCCCTTC 
ATGGAATTCC TGCTGCCTGT AGTTGTGAAG CTCTTCCCCA ACATGCTGCC CTCTACATTT 
GAGACCCAGT CTATCAAGGA GGAAAGGCTG AAGAAGGAGC TGAGGGTGAA GCTGGAGCTG 
GCCAAGTTTC TCCAGGACAC CATAGAGGAA ATGGCCCTGA AGAACAAGGC AGCCAAAGGG 
AATGCCACCA AAGATTTCTC TGCATTTTTC CAGAAGATCC GGGAGACAGG GGAGAGACCC 
AGCAATGAGG AAATCATGCG TTTTTCCAAA CTGTTTGAGG ATGAACTGAC CCTGGATAAC 
CTCACGAGGC CTCAGCTGGT AGCACTGTGC AAGCTGCTGG AGCTTCAGTC CATTGGCACC 
AACAACTTCC TGCGTTTCCA GCTCACCATG CGCCTGAGGT CCATAAAGGC TGATGATAAG 
CTGATTTCTG AGGAAGGGGT GGACAGTCTG ACTGTAAAAG AATTGCAGGC AGCATGTCGA 
GCACGGGGCA TGAGAGCTCT CGGTGTCACA GAAGACCGTC TGAAGGGCCA GCTGAAACAG 
TGGCTGGACT TGCACCTGCA TCACGAGATC CCCACATCAT TGCTCATACT GTCCCGGGCC 
ATGTACCTCC CAGACACCCT CTCACCTGCT GACCAGCTGA AGTCCACCTT GCAGACCCTT 
CCAGAAATTG TGGCAAAGGA GGCCCAGGTG AAAGTGGCCG AGGTGGAGGG CGAAAAGGTA 
GACAACAAGG CGAAGCTGGA GGCCACACTG CAGGAAGAGG CCGCCATCCA GCAAGAACAC 
CTGGAAGAAC TAAAGAGAGC CTCTGAGGCT GTGAAGGACA TCCAGCCAGA AGTAGCGGAA 
GCCACTCTCC CTGGGAGGCC AGGGCCTGAG CCTCAGCCTC CAGTGGATGA TGTGATCCTG 
CCCTCAGAGG TCCTGACGGA TACGGCACCT GTACTGGAAG GTCTGAAGGG TGAAGAGATT 
ACCAAGGAGG AGATTGACAT CCTCAGTGAC GCCTGCTCCA AGCTGCAGGA GCAGAAGAAG 
TCCCTGACCA AGGAGAAGGA GGAGCTGGAG CTGCTCAAAG AGGACGTCCA GGACTACAGT 
GAGGACTTAC AGGAGATCAA GAAGGAACTT TCAAAGACGG GTGAGGAGAA GTACATAGAA 
GAATCTGCAG CGAGCAAGAG GCTGTCAAAG CGAGTGCAGC AGATGATCGG GCAGATCGAT 
GGCCTGATCA CACAGCTGGA GACCACTCAA CAGGATGGCA AGCTGGGCCC CAGCCAGAGC 
ACTCCAACAG GGGAGAGTGT CATCAGCATC ACTGAGCTCA TCAGCGCCAT GAAGCAAATC 
AAGCACATTC CAGAGCACAA GCTCATCAGT TTGACCTCAG CCCTGGATGA TAATAAGGAC 
GGCAATATCA ACATCGATGA CCTTGTCAAG GTAATCGACT TGGTGAACAA AGAAGATGTT 
CAGATCTCTA CCACCCAGGT AGCTGAAATT GTGGCCACAC TAGAGAAAGA AGAAAAGATA 
GAAGAGAAAG AGAAGGCCAA GGAGAAGGCT GAGAAAGAGG CCGCAGAAGT GAAGAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Letm1 ( NM_019694.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062668.1 Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), mRNA.

Price & Availability↑

CloneID	OMu14659
Clone ID Related Accession (Same CDS sequence)	NM_019694.1 , NM_019694.2
Accession Version	NM_019694.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	mitochondrial proton/calcium exchanger protein precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF061026.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF061026.1, AK133849.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGCGTCCA TCTTGCTCAG GAGCTGCCGG GGCCGGGGGC CTGCCCGCCT CGCGCCACCT 
CGGGCCGCCT CCCCGCGGGG TAGTCTGAGG GATCGAGCTT GTCTCAGCTG TACCAGGACC 
CTGGGGTTGA CGAGCCGTGA GAGTGTTCTG TCTCGTTGCT GTACTCCAGC CCACCCTGTG 
TACCTCTGCT TCAAAGGTGA GCCCCTCAGC TGTTGGACTC AGAGGCCTGA GTGCCAGGGC 
ACCGCAGCAA GAACAACATG GACACCTGCC TCTGCAAGGC TGGTGGTTAC GGGACCTCAG 
TACCTTCCTG TGCGCGGCTG GCACTCATCA TCTCCGCTAG GAGAGGACTC TGTGATAGAG 
AAGTCCCTTA AGTCCTTAAA AGACAAGAAT AAGAAGCTGG AGGAGGGCGG CCCCGTGTAC 
AGCCCGCCCG CGCAGGTGGT GGTGAGGAAA TCCCTCGGGC AGAAGGTACT GGATGAGCTG 
AGGCACTACT ACCATGGCTT CCGCCTGCTC TGGATCGACA CCAAGATCGC TGCCCGGATG 
CTCTGGCGCA TCCTCAATGG CCATACGCTG ACCCGCCGGG AGCGCAGGCA GTTTCTCCGG 
ATTTGTGCGG ACCTCTTCCG TCTAGTGCCC TTCCTGGTGT TCGTGGTGGT GCCCTTCATG 
GAATTCCTGC TGCCTGTAGT TGTGAAGCTC TTCCCCAACA TGCTGCCCTC TACATTTGAG 
ACCCAGTCTA TCAAGGAGGA AAGGCTGAAG AAGGAGCTGA GGGTGAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT AGAGGAAATG GCCCTGAAGA ACAAGGCAGC CAAAGGGAAT 
GCCACCAAAG ATTTCTCTGC ATTTTTCCAG AAGATCCGGG AGACAGGGGA GAGACCCAGC 
AATGAGGAAA TCATGCGTTT TTCCAAACTG TTTGAGGATG AACTGACCCT GGATAACCTC 
ACGAGGCCTC AGCTGGTAGC ACTGTGCAAG CTGCTGGAGC TTCAGTCCAT TGGCACCAAC 
AACTTCCTGC GTTTCCAGCT CACCATGCGC CTGAGGTCCA TAAAGGCTGA TGATAAGCTG 
ATTTCTGAGG AAGGGGTGGA CAGTCTGACT GTAAAAGAAT TGCAGGCAGC ATGTCGAGCA 
CGGGGCATGA GAGCTCTCGG TGTCACAGAA GACCGTCTGA AGGGCCAGCT GAAACAGTGG 
CTGGACTTGC ACCTGCATCA CGAGATCCCC ACATCATTGC TCATACTGTC CCGGGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCTGAC CAGCTGAAGT CCACCTTGCA GACCCTTCCA 
GAAATTGTGG CAAAGGAGGC CCAGGTGAAA GTGGCCGAGG TGGAGGGCGA AAAGGTAGAC 
AACAAGGCGA AGCTGGAGGC CACACTGCAG GAAGAGGCCG CCATCCAGCA AGAACACCTG 
GAAGAACTAA AGAGAGCCTC TGAGGCTGTG AAGGACATCC AGCCAGAAGT AGCGGAAGCC 
ACTCTCCCTG GGAGGCCAGG GCCTGAGCCT CAGCCTCCAG TGGATGATGT GATCCTGCCC 
TCAGAGGTCC TGACGGATAC GGCACCTGTA CTGGAAGGTC TGAAGGGTGA AGAGATTACC 
AAGGAGGAGA TTGACATCCT CAGTGACGCC TGCTCCAAGC TGCAGGAGCA GAAGAAGTCC 
CTGACCAAGG AGAAGGAGGA GCTGGAGCTG CTCAAAGAGG ACGTCCAGGA CTACAGTGAG 
GACTTACAGG AGATCAAGAA GGAACTTTCA AAGACGGGTG AGGAGAAGTA CATAGAAGAA 
TCTGCAGCGA GCAAGAGGCT GTCAAAGCGA GTGCAGCAGA TGATCGGGCA GATCGATGGC 
CTGATCACAC AGCTGGAGAC CACTCAACAG GATGGCAAGC TGGGCCCCAG CCAGAGCACT 
CCAACAGGGG AGAGTGTCAT CAGCATCACT GAGCTCATCA GCGCCATGAA GCAAATCAAG 
CACATTCCAG AGCACAAGCT CATCAGTTTG ACCTCAGCCC TGGATGATAA TAAGGACGGC 
AATATCAACA TCGATGACCT TGTCAAGGTA ATCGACTTGG TGAACAAAGA AGATGTTCAG 
ATCTCTACCA CCCAGGTAGC TGAAATTGTG GCCACACTAG AGAAAGAAGA AAAGATAGAA 
GAGAAAGAGA AGGCCAAGGA GAAGGCTGAG AAAGAGGCCG CAGAAGTGAA GAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062668.1
CDS	153..2369
Translation	MASILLRSCRGRGPARLAPPRAASPRGSLRDRACLSCTRTLGLTSRESVLSRCCTPAHPVYLCFKGEPLSCWTQRPECQGTAARTTWTPASARLVVTGPQYLPVRGWHSSSPLGEDSVIEKSLKSLKDKNKKLEEGGPVYSPPAQVVVRKSLGQKVLDELRHYYHGFRLLWIDTKIAARMLWRILNGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVVVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSAFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLISEEGVDSLTVKELQAACRARGMRALGVTEDRLKGQLKQWLDLHLHHEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEKVDNKAKLEATLQEEAAIQQEHLEELKRASEAVKDIQPEVAEATLPGRPGPEPQPPVDDVILPSEVLTDTAPVLEGLKGEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYIEESAASKRLSKRVQQMIGQIDGLITQLETTQQDGKLGPSQSTPTGESVISITELISAMKQIKHIPEHKLISLTSALDDNKDGNINIDDLVKVIDLVNKEDVQISTTQVAEIVATLEKEEKIEEKEKAKEKAEKEAAEVKN

Target ORF information:

RefSeq Version	NM_019694.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019694.1

ORF Insert Sequence:

ATGGCGTCCA TCTTGCTCAG GAGCTGCCGG GGCCGGGGGC CTGCCCGCCT CGCGCCACCT 
CGGGCCGCCT CCCCGCGGGG TAGTCTGAGG GATCGAGCTT GTCTCAGCTG TACCAGGACC 
CTGGGGTTGA CGAGCCGTGA GAGTGTTCTG TCTCGTTGCT GTACTCCAGC CCACCCTGTG 
TACCTCTGCT TCAAAGGTGA GCCCCTCAGC TGTTGGACTC AGAGGCCTGA GTGCCAGGGC 
ACCGCAGCAA GAACAACATG GACACCTGCC TCTGCAAGGC TGGTGGTTAC GGGACCTCAG 
TACCTTCCTG TGCGCGGCTG GCACTCATCA TCTCCGCTAG GAGAGGACTC TGTGATAGAG 
AAGTCCCTTA AGTCCTTAAA AGACAAGAAT AAGAAGCTGG AGGAGGGCGG CCCCGTGTAC 
AGCCCGCCCG CGCAGGTGGT GGTGAGGAAA TCCCTCGGGC AGAAGGTACT GGATGAGCTG 
AGGCACTACT ACCATGGCTT CCGCCTGCTC TGGATCGACA CCAAGATCGC TGCCCGGATG 
CTCTGGCGCA TCCTCAATGG CCATACGCTG ACCCGCCGGG AGCGCAGGCA GTTTCTCCGG 
ATTTGTGCGG ACCTCTTCCG TCTAGTGCCC TTCCTGGTGT TCGTGGTGGT GCCCTTCATG 
GAATTCCTGC TGCCTGTAGT TGTGAAGCTC TTCCCCAACA TGCTGCCCTC TACATTTGAG 
ACCCAGTCTA TCAAGGAGGA AAGGCTGAAG AAGGAGCTGA GGGTGAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT AGAGGAAATG GCCCTGAAGA ACAAGGCAGC CAAAGGGAAT 
GCCACCAAAG ATTTCTCTGC ATTTTTCCAG AAGATCCGGG AGACAGGGGA GAGACCCAGC 
AATGAGGAAA TCATGCGTTT TTCCAAACTG TTTGAGGATG AACTGACCCT GGATAACCTC 
ACGAGGCCTC AGCTGGTAGC ACTGTGCAAG CTGCTGGAGC TTCAGTCCAT TGGCACCAAC 
AACTTCCTGC GTTTCCAGCT CACCATGCGC CTGAGGTCCA TAAAGGCTGA TGATAAGCTG 
ATTTCTGAGG AAGGGGTGGA CAGTCTGACT GTAAAAGAAT TGCAGGCAGC ATGTCGAGCA 
CGGGGCATGA GAGCTCTCGG TGTCACAGAA GACCGTCTGA AGGGCCAGCT GAAACAGTGG 
CTGGACTTGC ACCTGCATCA CGAGATCCCC ACATCATTGC TCATACTGTC CCGGGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCTGAC CAGCTGAAGT CCACCTTGCA GACCCTTCCA 
GAAATTGTGG CAAAGGAGGC CCAGGTGAAA GTGGCCGAGG TGGAGGGCGA AAAGGTAGAC 
AACAAGGCGA AGCTGGAGGC CACACTGCAG GAAGAGGCCG CCATCCAGCA AGAACACCTG 
GAAGAACTAA AGAGAGCCTC TGAGGCTGTG AAGGACATCC AGCCAGAAGT AGCGGAAGCC 
ACTCTCCCTG GGAGGCCAGG GCCTGAGCCT CAGCCTCCAG TGGATGATGT GATCCTGCCC 
TCAGAGGTCC TGACGGATAC GGCACCTGTA CTGGAAGGTC TGAAGGGTGA AGAGATTACC 
AAGGAGGAGA TTGACATCCT CAGTGACGCC TGCTCCAAGC TGCAGGAGCA GAAGAAGTCC 
CTGACCAAGG AGAAGGAGGA GCTGGAGCTG CTCAAAGAGG ACGTCCAGGA CTACAGTGAG 
GACTTACAGG AGATCAAGAA GGAACTTTCA AAGACGGGTG AGGAGAAGTA CATAGAAGAA 
TCTGCAGCGA GCAAGAGGCT GTCAAAGCGA GTGCAGCAGA TGATCGGGCA GATCGATGGC 
CTGATCACAC AGCTGGAGAC CACTCAACAG GATGGCAAGC TGGGCCCCAG CCAGAGCACT 
CCAACAGGGG AGAGTGTCAT CAGCATCACT GAGCTCATCA GCGCCATGAA GCAAATCAAG 
CACATTCCAG AGCACAAGCT CATCAGTTTG ACCTCAGCCC TGGATGATAA TAAGGACGGC 
AATATCAACA TCGATGACCT TGTCAAGGTA ATCGACTTGG TGAACAAAGA AGATGTTCAG 
ATCTCTACCA CCCAGGTAGC TGAAATTGTG GCCACACTAG AGAAAGAAGA AAAGATAGAA 
GAGAAAGAGA AGGCCAAGGA GAAGGCTGAG AAAGAGGCCG CAGAAGTGAA GAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Letm1 ( NM_019694.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_062668.1 Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), mRNA.

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CloneID	OMu14659
Clone ID Related Accession (Same CDS sequence)	NM_019694.1 , NM_019694.2
Accession Version	NM_019694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Mus musculus(house mouse)
Product	mitochondrial proton/calcium exchanger protein precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY110340.1, AF061026.1, BQ178952.1, AK163499.1 and BY243359.1. On May 17, 2018 this sequence version replaced NM_019694.1. ##Evidence-Data-START## Transcript exon combination :: BC061115.1, BC061690.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164138, SAMN01164142 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCA TCTTGCTCAG GAGCTGCCGG GGCCGGGGGC CTGCCCGCCT CGCGCCACCT 
CGGGCCGCCT CCCCGCGGGG TAGTCTGAGG GATCGAGCTT GTCTCAGCTG TACCAGGACC 
CTGGGGTTGA CGAGCCGTGA GAGTGTTCTG TCTCGTTGCT GTACTCCAGC CCACCCTGTG 
TACCTCTGCT TCAAAGGTGA GCCCCTCAGC TGTTGGACTC AGAGGCCTGA GTGCCAGGGC 
ACCGCAGCAA GAACAACATG GACACCTGCC TCTGCAAGGC TGGTGGTTAC GGGACCTCAG 
TACCTTCCTG TGCGCGGCTG GCACTCATCA TCTCCGCTAG GAGAGGACTC TGTGATAGAG 
AAGTCCCTTA AGTCCTTAAA AGACAAGAAT AAGAAGCTGG AGGAGGGCGG CCCCGTGTAC 
AGCCCGCCCG CGCAGGTGGT GGTGAGGAAA TCCCTCGGGC AGAAGGTACT GGATGAGCTG 
AGGCACTACT ACCATGGCTT CCGCCTGCTC TGGATCGACA CCAAGATCGC TGCCCGGATG 
CTCTGGCGCA TCCTCAATGG CCATACGCTG ACCCGCCGGG AGCGCAGGCA GTTTCTCCGG 
ATTTGTGCGG ACCTCTTCCG TCTAGTGCCC TTCCTGGTGT TCGTGGTGGT GCCCTTCATG 
GAATTCCTGC TGCCTGTAGT TGTGAAGCTC TTCCCCAACA TGCTGCCCTC TACATTTGAG 
ACCCAGTCTA TCAAGGAGGA AAGGCTGAAG AAGGAGCTGA GGGTGAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT AGAGGAAATG GCCCTGAAGA ACAAGGCAGC CAAAGGGAAT 
GCCACCAAAG ATTTCTCTGC ATTTTTCCAG AAGATCCGGG AGACAGGGGA GAGACCCAGC 
AATGAGGAAA TCATGCGTTT TTCCAAACTG TTTGAGGATG AACTGACCCT GGATAACCTC 
ACGAGGCCTC AGCTGGTAGC ACTGTGCAAG CTGCTGGAGC TTCAGTCCAT TGGCACCAAC 
AACTTCCTGC GTTTCCAGCT CACCATGCGC CTGAGGTCCA TAAAGGCTGA TGATAAGCTG 
ATTTCTGAGG AAGGGGTGGA CAGTCTGACT GTAAAAGAAT TGCAGGCAGC ATGTCGAGCA 
CGGGGCATGA GAGCTCTCGG TGTCACAGAA GACCGTCTGA AGGGCCAGCT GAAACAGTGG 
CTGGACTTGC ACCTGCATCA CGAGATCCCC ACATCATTGC TCATACTGTC CCGGGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCTGAC CAGCTGAAGT CCACCTTGCA GACCCTTCCA 
GAAATTGTGG CAAAGGAGGC CCAGGTGAAA GTGGCCGAGG TGGAGGGCGA AAAGGTAGAC 
AACAAGGCGA AGCTGGAGGC CACACTGCAG GAAGAGGCCG CCATCCAGCA AGAACACCTG 
GAAGAACTAA AGAGAGCCTC TGAGGCTGTG AAGGACATCC AGCCAGAAGT AGCGGAAGCC 
ACTCTCCCTG GGAGGCCAGG GCCTGAGCCT CAGCCTCCAG TGGATGATGT GATCCTGCCC 
TCAGAGGTCC TGACGGATAC GGCACCTGTA CTGGAAGGTC TGAAGGGTGA AGAGATTACC 
AAGGAGGAGA TTGACATCCT CAGTGACGCC TGCTCCAAGC TGCAGGAGCA GAAGAAGTCC 
CTGACCAAGG AGAAGGAGGA GCTGGAGCTG CTCAAAGAGG ACGTCCAGGA CTACAGTGAG 
GACTTACAGG AGATCAAGAA GGAACTTTCA AAGACGGGTG AGGAGAAGTA CATAGAAGAA 
TCTGCAGCGA GCAAGAGGCT GTCAAAGCGA GTGCAGCAGA TGATCGGGCA GATCGATGGC 
CTGATCACAC AGCTGGAGAC CACTCAACAG GATGGCAAGC TGGGCCCCAG CCAGAGCACT 
CCAACAGGGG AGAGTGTCAT CAGCATCACT GAGCTCATCA GCGCCATGAA GCAAATCAAG 
CACATTCCAG AGCACAAGCT CATCAGTTTG ACCTCAGCCC TGGATGATAA TAAGGACGGC 
AATATCAACA TCGATGACCT TGTCAAGGTA ATCGACTTGG TGAACAAAGA AGATGTTCAG 
ATCTCTACCA CCCAGGTAGC TGAAATTGTG GCCACACTAG AGAAAGAAGA AAAGATAGAA 
GAGAAAGAGA AGGCCAAGGA GAAGGCTGAG AAAGAGGCCG CAGAAGTGAA GAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062668.1
CDS	166..2382
Translation	MASILLRSCRGRGPARLAPPRAASPRGSLRDRACLSCTRTLGLTSRESVLSRCCTPAHPVYLCFKGEPLSCWTQRPECQGTAARTTWTPASARLVVTGPQYLPVRGWHSSSPLGEDSVIEKSLKSLKDKNKKLEEGGPVYSPPAQVVVRKSLGQKVLDELRHYYHGFRLLWIDTKIAARMLWRILNGHTLTRRERRQFLRICADLFRLVPFLVFVVVPFMEFLLPVVVKLFPNMLPSTFETQSIKEERLKKELRVKLELAKFLQDTIEEMALKNKAAKGNATKDFSAFFQKIRETGERPSNEEIMRFSKLFEDELTLDNLTRPQLVALCKLLELQSIGTNNFLRFQLTMRLRSIKADDKLISEEGVDSLTVKELQAACRARGMRALGVTEDRLKGQLKQWLDLHLHHEIPTSLLILSRAMYLPDTLSPADQLKSTLQTLPEIVAKEAQVKVAEVEGEKVDNKAKLEATLQEEAAIQQEHLEELKRASEAVKDIQPEVAEATLPGRPGPEPQPPVDDVILPSEVLTDTAPVLEGLKGEEITKEEIDILSDACSKLQEQKKSLTKEKEELELLKEDVQDYSEDLQEIKKELSKTGEEKYIEESAASKRLSKRVQQMIGQIDGLITQLETTQQDGKLGPSQSTPTGESVISITELISAMKQIKHIPEHKLISLTSALDDNKDGNINIDDLVKVIDLVNKEDVQISTTQVAEIVATLEKEEKIEEKEKAKEKAEKEAAEVKN

Target ORF information:

RefSeq Version	NM_019694.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus leucine zipper-EF-hand containing transmembrane protein 1 (Letm1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019694.2

ORF Insert Sequence:

ATGGCGTCCA TCTTGCTCAG GAGCTGCCGG GGCCGGGGGC CTGCCCGCCT CGCGCCACCT 
CGGGCCGCCT CCCCGCGGGG TAGTCTGAGG GATCGAGCTT GTCTCAGCTG TACCAGGACC 
CTGGGGTTGA CGAGCCGTGA GAGTGTTCTG TCTCGTTGCT GTACTCCAGC CCACCCTGTG 
TACCTCTGCT TCAAAGGTGA GCCCCTCAGC TGTTGGACTC AGAGGCCTGA GTGCCAGGGC 
ACCGCAGCAA GAACAACATG GACACCTGCC TCTGCAAGGC TGGTGGTTAC GGGACCTCAG 
TACCTTCCTG TGCGCGGCTG GCACTCATCA TCTCCGCTAG GAGAGGACTC TGTGATAGAG 
AAGTCCCTTA AGTCCTTAAA AGACAAGAAT AAGAAGCTGG AGGAGGGCGG CCCCGTGTAC 
AGCCCGCCCG CGCAGGTGGT GGTGAGGAAA TCCCTCGGGC AGAAGGTACT GGATGAGCTG 
AGGCACTACT ACCATGGCTT CCGCCTGCTC TGGATCGACA CCAAGATCGC TGCCCGGATG 
CTCTGGCGCA TCCTCAATGG CCATACGCTG ACCCGCCGGG AGCGCAGGCA GTTTCTCCGG 
ATTTGTGCGG ACCTCTTCCG TCTAGTGCCC TTCCTGGTGT TCGTGGTGGT GCCCTTCATG 
GAATTCCTGC TGCCTGTAGT TGTGAAGCTC TTCCCCAACA TGCTGCCCTC TACATTTGAG 
ACCCAGTCTA TCAAGGAGGA AAGGCTGAAG AAGGAGCTGA GGGTGAAGCT GGAGCTGGCC 
AAGTTTCTCC AGGACACCAT AGAGGAAATG GCCCTGAAGA ACAAGGCAGC CAAAGGGAAT 
GCCACCAAAG ATTTCTCTGC ATTTTTCCAG AAGATCCGGG AGACAGGGGA GAGACCCAGC 
AATGAGGAAA TCATGCGTTT TTCCAAACTG TTTGAGGATG AACTGACCCT GGATAACCTC 
ACGAGGCCTC AGCTGGTAGC ACTGTGCAAG CTGCTGGAGC TTCAGTCCAT TGGCACCAAC 
AACTTCCTGC GTTTCCAGCT CACCATGCGC CTGAGGTCCA TAAAGGCTGA TGATAAGCTG 
ATTTCTGAGG AAGGGGTGGA CAGTCTGACT GTAAAAGAAT TGCAGGCAGC ATGTCGAGCA 
CGGGGCATGA GAGCTCTCGG TGTCACAGAA GACCGTCTGA AGGGCCAGCT GAAACAGTGG 
CTGGACTTGC ACCTGCATCA CGAGATCCCC ACATCATTGC TCATACTGTC CCGGGCCATG 
TACCTCCCAG ACACCCTCTC ACCTGCTGAC CAGCTGAAGT CCACCTTGCA GACCCTTCCA 
GAAATTGTGG CAAAGGAGGC CCAGGTGAAA GTGGCCGAGG TGGAGGGCGA AAAGGTAGAC 
AACAAGGCGA AGCTGGAGGC CACACTGCAG GAAGAGGCCG CCATCCAGCA AGAACACCTG 
GAAGAACTAA AGAGAGCCTC TGAGGCTGTG AAGGACATCC AGCCAGAAGT AGCGGAAGCC 
ACTCTCCCTG GGAGGCCAGG GCCTGAGCCT CAGCCTCCAG TGGATGATGT GATCCTGCCC 
TCAGAGGTCC TGACGGATAC GGCACCTGTA CTGGAAGGTC TGAAGGGTGA AGAGATTACC 
AAGGAGGAGA TTGACATCCT CAGTGACGCC TGCTCCAAGC TGCAGGAGCA GAAGAAGTCC 
CTGACCAAGG AGAAGGAGGA GCTGGAGCTG CTCAAAGAGG ACGTCCAGGA CTACAGTGAG 
GACTTACAGG AGATCAAGAA GGAACTTTCA AAGACGGGTG AGGAGAAGTA CATAGAAGAA 
TCTGCAGCGA GCAAGAGGCT GTCAAAGCGA GTGCAGCAGA TGATCGGGCA GATCGATGGC 
CTGATCACAC AGCTGGAGAC CACTCAACAG GATGGCAAGC TGGGCCCCAG CCAGAGCACT 
CCAACAGGGG AGAGTGTCAT CAGCATCACT GAGCTCATCA GCGCCATGAA GCAAATCAAG 
CACATTCCAG AGCACAAGCT CATCAGTTTG ACCTCAGCCC TGGATGATAA TAAGGACGGC 
AATATCAACA TCGATGACCT TGTCAAGGTA ATCGACTTGG TGAACAAAGA AGATGTTCAG 
ATCTCTACCA CCCAGGTAGC TGAAATTGTG GCCACACTAG AGAAAGAAGA AAAGATAGAA 
GAGAAAGAGA AGGCCAAGGA GAAGGCTGAG AAAGAGGCCG CAGAAGTGAA GAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PINK1-mediated phosphorylation of LETM1 regulates mitochondrial calcium transport and protects neurons against mitochondrial stress.
Nature communications8(1)1399(2017 Nov)
Huang E,Qu D,Huang T,Rizzi N,Boonying W,Krolak D,Ciana P,Woulfe J,Klein C,Slack RS,Figeys D,Park DS

Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) forms a Ca
Scientific reports634174(2016 Sep)
Shao J,Fu Z,Ji Y,Guan X,Guo S,Ding Z,Yang X,Cong Y,Shen Y

LETM1-dependent mitochondrial Ca2+ flux modulates cellular bioenergetics and proliferation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(11)4936-49(2014 Nov)
Doonan PJ,Chandramoorthy HC,Hoffman NE,Zhang X,C?rdenas C,Shanmughapriya S,Rajan S,Vallem S,Chen X,Foskett JK,Cheung JY,Houser SR,Madesh M

Letm1, the mitochondrial Ca2+/H+ antiporter, is essential for normal glucose metabolism and alters brain function in Wolf-Hirschhorn syndrome.
Proceedings of the National Academy of Sciences of the United States of America110(24)E2249-54(2013 Jun)
Jiang D,Zhao L,Clish CB,Clapham DE

Endoplasmic reticulum potassium-hydrogen exchanger and small conductance calcium-activated potassium channel activities are essential for ER calcium uptake in neurons and cardiomyocytes.
Journal of cell science125(Pt 3)625-33(2012 Feb)
Kuum M,Veksler V,Liiv J,Ventura-Clapier R,Kaasik A

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Letm1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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